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Pesquisa : B03.440.400.425.293.150 [Categoria DeCS]
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[PMID]:28431244
[Au] Autor:García-González AP; Ritter AD; Shrestha S; Andersen EC; Yilmaz LS; Walhout AJM
[Ad] Endereço:Program in Systems Biology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
[Ti] Título:Bacterial Metabolism Affects the C. elegans Response to Cancer Chemotherapeutics.
[So] Source:Cell;169(3):431-441.e8, 2017 Apr 20.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The human microbiota greatly affects physiology and disease; however, the contribution of bacteria to the response to chemotherapeutic drugs remains poorly understood. Caenorhabditis elegans and its bacterial diet provide a powerful system to study host-bacteria interactions. Here, we use this system to study how bacteria affect the C. elegans response to chemotherapeutics. We find that different bacterial species can increase the response to one drug yet decrease the effect of another. We perform genetic screens in two bacterial species using three chemotherapeutic drugs: 5-fluorouracil (5-FU), 5-fluoro-2'-deoxyuridine (FUDR), and camptothecin (CPT). We find numerous bacterial nucleotide metabolism genes that affect drug efficacy in C. elegans. Surprisingly, we find that 5-FU and FUDR act through bacterial ribonucleotide metabolism to elicit their cytotoxic effects in C. elegans rather than by thymineless death or DNA damage. Our study provides a blueprint for characterizing the role of bacteria in the host response to chemotherapeutics.
[Mh] Termos MeSH primário: Antineoplásicos/metabolismo
Caenorhabditis elegans/microbiologia
Comamonas/metabolismo
Escherichia coli/metabolismo
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacologia
Camptotecina/metabolismo
Camptotecina/farmacologia
Neoplasias Colorretais/tratamento farmacológico
Comamonas/genética
Desoxiuridina/análogos & derivados
Desoxiuridina/metabolismo
Desoxiuridina/farmacologia
Dieta
Escherichia coli/genética
Fluoruracila/metabolismo
Fluoruracila/farmacologia
Seres Humanos
Modelos Animais
Nucleosídeos de Pirimidina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-fluoro-2'-deoxyuridine); 0 (Antineoplastic Agents); 0 (Pyrimidine Nucleosides); U3P01618RT (Fluorouracil); W78I7AY22C (Deoxyuridine); XT3Z54Z28A (Camptothecin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE


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[PMID]:28281889
[Au] Autor:Li CH; Ye C; Hou XP; Chen MH; Zheng XY; Cai XY
[Ad] Endereço:a State Key Laboratory of Environmental Criteria and Risk Assessment , Chinese Research Academy of Environmental Sciences , Beijing , China.
[Ti] Título:Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria with tolerance to hypoxic environments.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;52(7):581-589, 2017 Jun 07.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hypoxic conditions are considerably different from aerobic and anaerobic conditions, and they are widely distributed in natural environments. Many pollutants, including polycyclic aromatic hydrocarbons (PAHs), tend to accumulate in hypoxic environments. However, PAH biodegradation under hypoxic conditions is poorly understood compared with that under obligate aerobic and obligate anaerobic conditions. In the present study, PAH-degrading bacteria were enriched, and their biodegradation rates were tested using a hypoxic station with an 8% oxygen concentration. PAH-degrading bacteria collected from sediments in low-oxygen environments were enriched using phenanthrene (Phe) or pyrene (Pyr) as the sole carbon and energy source. Individual bacterial colonies showing the ability to degrade Phe or Pyr were isolated and identified by 16S rDNA gene sequencing. Morphological and physiological characterizations of the isolated bacterial colonies were performed. The isolated bacteria were observed by scanning electron microscopy (SEM) and were identified as Pseudomonas sp., Klebsiella sp., Bacillus sp., and Comamonas sp. Phylogenetic tree of the isolated PAH-degrading bacteria was also constructed. The biodegradation ability of these bacteria was tested at an initial Phe or Pyr concentration of 50 mg L . The biodegradation kinetics were best fit by a first-order rate model and presented regression coefficients (r ) that varied from 0.7728 to 0.9725 (P < 0.05). The half-lives of the PAHs varied from 2.99 to 3.65 d for Phe and increased to 60.3-82.5 d for Pyr. These half-lives were much shorter than those observed under anaerobic conditions but were similar to those observed under aerobic conditions.
[Mh] Termos MeSH primário: Sedimentos Geológicos/análise
Sedimentos Geológicos/microbiologia
Hidrocarbonetos Aromáticos Policíclicos/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Anaerobiose
Bacillus/isolamento & purificação
Bacillus/fisiologia
Biodegradação Ambiental
China
Comamonas/isolamento & purificação
Comamonas/fisiologia
DNA Ribossômico/genética
Klebsiella/isolamento & purificação
Klebsiella/fisiologia
Oxigênio/análise
Filogenia
Pseudomonas/isolamento & purificação
Pseudomonas/fisiologia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (Polycyclic Aromatic Hydrocarbons); 0 (RNA, Ribosomal, 16S); 0 (Water Pollutants, Chemical); S88TT14065 (Oxygen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2017.1293991


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[PMID]:27900438
[Au] Autor:Liu T; Mao YJ; Shi YP; Quan X
[Ad] Endereço:Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, People's Republic of China. taoliu@dlut.edu.cn.
[Ti] Título:Start-up and bacterial community compositions of partial nitrification in moving bed biofilm reactor.
[So] Source:Appl Microbiol Biotechnol;101(6):2563-2574, 2017 Mar.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Partial nitrification (PN) has been considered as one of the promising processes for pretreatment of ammonium-rich wastewater. In this study, a kind of novel carriers with enhanced hydrophilicity and electrophilicity was implemented in a moving bed biofilm reactor (MBBR) to start up PN process. Results indicated that biofilm formation rate was higher on modified carriers. In comparison with the reactor filled with traditional carriers (start-up period of 21 days), it took only 14 days to start up PN successfully with ammonia removal efficiency and nitrite accumulation rate of 90 and 91%, respectively, in the reactor filled with modified carriers. Evident changes of spatial distributions and community structures had been detected during the start-up. Free-floating cells existed in planktonic sludge, while these microorganisms trended to form flocs in the biofilm. High-throughput pyrosequencing results indicated that Nitrosomonas was the predominant ammonia-oxidizing bacterium (AOB) in the PN system, while Comamonas might also play a vital role for nitrogen oxidation. Additionally, some other bacteria such as Ferruginibacter, Ottowia, Saprospiraceae, and Rhizobacter were selected to establish stable footholds. This study would be potentially significant for better understanding the microbial features and developing efficient strategies accordingly for MBBR-based PN operation.
[Mh] Termos MeSH primário: Amônia/metabolismo
Bactérias/genética
Biofilmes/crescimento & desenvolvimento
Filogenia
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Bactérias/classificação
Bactérias/metabolismo
Reatores Biológicos
Comamonas/crescimento & desenvolvimento
Comamonas/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Consórcios Microbianos/fisiologia
Nitrificação
Nitrosomonas/crescimento & desenvolvimento
Nitrosomonas/metabolismo
Oxirredução
Eliminação de Resíduos Líquidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); 7664-41-7 (Ammonia)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-8003-9


  4 / 173 MEDLINE  
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[PMID]:27363842
[Au] Autor:Coon KL; Brown MR; Strand MR
[Ad] Endereço:Department of Entomology, The University of Georgia, 120 Cedar Street, 420 Biological Sciences, Athens, GA, 30602, USA.
[Ti] Título:Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae).
[So] Source:Parasit Vectors;9(1):375, 2016 06 30.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Aedes aegypti and A. atropalpus are related mosquitoes that differ reproductively. Aedes aegypti must blood-feed to produce eggs (anautogenous) while A. atropalpus always produces a first clutch of eggs without blood-feeding (facultatively autogenous). We recently characterized the gut microbiota of A. aegypti and A. atropalpus that were reared identically in the laboratory. Here, we assessed the effects of specific members of the gut microbiota in A. aegypti and A. atropalpus on female fitness including egg production. METHODS: Gnotobiotic A. aegypti and A. atropalpus larvae were colonized by specific members of the gut microbiota. Survival, development time, size and egg production for each treatment was then compared to axenic and conventionally reared larvae. RESULTS: Most species of bacteria we tested supported normal development and egg production by A. aegypti but only one betaproteobacterium, a Comamonas, supported development and egg production by A. atropalpus to equivalent levels as conventionally reared females. Aedes atropalpus females colonized by Comamonas contained similar stores of glycogen and protein as conventionally reared females, whereas females colonized by Aquitalea did not. Small differences in bacterial loads were detected between gnotobiotic and conventionally reared A. aegypti and A. atropalpus, but this variation did not correlate with the beneficial effects of Comamonas in A. atropalpus. CONCLUSIONS: Specific members of the gut microbiota more strongly affected survival, size and egg production by A. atropalpus than A. aegypti.
[Mh] Termos MeSH primário: Aedes/microbiologia
Comamonas/fisiologia
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Aedes/fisiologia
Animais
Feminino
Oviposição/fisiologia
Óvulo/fisiologia
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170303
[Lr] Data última revisão:
170303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1660-9


  5 / 173 MEDLINE  
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[PMID]:27289060
[Au] Autor:Jiang B; Tan L; Ning S; Shi S
[Ad] Endereço:School of Life Science, Liaoning Normal University, Dalian 116081, China; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
[Ti] Título:A novel integration system of magnetically immobilized cells and a pair of graphite plate-stainless iron mesh electrodes for the bioremediation of coking wastewater.
[So] Source:Bioresour Technol;216:684-90, 2016 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Magnetically immobilized cells of Comamonas sp. JB coupling with electrode reaction was developed to enhance the treatment efficiency of coking wastewater containing phenol, carbazole (CA), dibenzofuran (DBF), and dibenzothiophene (DBT). The pair of graphite plate-stainless iron mesh electrodes was chosen as the most suitable electrodes. Magnetically immobilized cells coupling with graphite plate-stainless iron mesh electrodes (coupling system) exhibited high degradation activity for all the compounds, which were significantly higher than the sum by single magnetically immobilized cells and electrode reaction at the optimal voltage. Recycling experiments demonstrated that the degradation activity of coupling system increased gradually during eight recycles, indicating that there was a coupling effect between the biodegradation and electrode reaction. Phenol hydroxylase and qPCR assays confirmed that appropriate electrical stimulation could improve phenol hydroxylase activity and promote cells growth. Toxicity assessment suggested the treatment of the coking wastewater by coupling system led to less toxicity than untreated wastewater.
[Mh] Termos MeSH primário: Coque
Comamonas/química
Eliminação de Resíduos Líquidos/métodos
Águas Residuais/química
[Mh] Termos MeSH secundário: Benzofuranos/química
Benzofuranos/metabolismo
Biodegradação Ambiental
Carbazóis/química
Carbazóis/metabolismo
Células Imobilizadas/química
Comamonas/metabolismo
Eletrodos
Grafite
Ferro
Magnetismo
Oxigenases de Função Mista/química
Oxigenases de Função Mista/metabolismo
Fenol/química
Fenol/metabolismo
Tiofenos/química
Tiofenos/metabolismo
Eliminação de Resíduos Líquidos/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzofurans); 0 (Carbazoles); 0 (Coke); 0 (Thiophenes); 0 (Waste Water); 0P2197HHHN (carbazole); 339NCG44TV (Phenol); 7782-42-5 (Graphite); 8U54U639VI (dibenzofuran); E1UOL152H7 (Iron); EC 1.- (Mixed Function Oxygenases); EC 1.14.13.7 (phenol 2-monooxygenase); Z3D4AJ1R48 (dibenzothiophene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE


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[PMID]:27120651
[Au] Autor:Dlangamandla C; Dyantyi SA; Mpentshu YP; Ntwampe SK; Basitere M
[Ad] Endereço:Faculty of Applied Sciences, Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, PO Box 652, Cape Town 8000, South Africa E-mail: NtwampeS@cput.ac.za.
[Ti] Título:Optimisation of bioflocculant production by a biofilm forming microorganism from poultry slaughterhouse wastewater for use in poultry wastewater treatment.
[So] Source:Water Sci Technol;73(8):1963-8, 2016.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Poultry slaughterhouse wastewater contains nutrients that are sufficient for microbial growth; moreover, the wastewater has microorganisms which can be harnessed to perform specific functions. Additionally, these microorganisms can grow either in planktonic (free floating) mode or sessile (attached) mode. This study focused on the optimisation of bioflocculant production by quantifying flocculation activity, determined using kaolin clay (4 g/L), by isolates prevalent in poultry slaughterhouse wastewater. Subsequent to their identification and characterisation, six bacterial strains were initially isolated from the poultry wastewater. Although all the isolated microorganisms produced bioflocculants under different conditions, i.e. pH and temperature, the strain that produced bioflocculants with a higher flocculation activity was isolate BF-3, a Comamonas sp., achieving a flocculation activity of 93.8% at 32.9 °C and pH 6.5. Fourier transform infrared spectroscopy (FTIR) analysis of the bioflocculant of the isolate, showed the presence of hydroxyl, carboxyl, alkane and amine functional groups, an indication that the bioflocculant was a protein constituent.
[Mh] Termos MeSH primário: Biofilmes
Comamonas/fisiologia
Aves Domésticas
Águas Residuais/química
[Mh] Termos MeSH secundário: Matadouros
Animais
Floculação
Resíduos Industriais
Caulim/química
Espectroscopia de Infravermelho com Transformada de Fourier
Temperatura Ambiente
Eliminação de Resíduos Líquidos/métodos
Poluentes Químicos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Industrial Waste); 0 (Waste Water); 0 (Water Pollutants, Chemical); 24H4NWX5CO (Kaolin)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160428
[Lr] Data última revisão:
160428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160428
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2016.047


  7 / 173 MEDLINE  
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[PMID]:26949053
[Au] Autor:Sindhu R; Kuttiraja M; Prabisha TP; Binod P; Sukumaran RK; Pandey A
[Ad] Endereço:Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India. Electronic address: sindhurgcb@gmail.com.
[Ti] Título:Development of a combined pretreatment and hydrolysis strategy of rice straw for the production of bioethanol and biopolymer.
[So] Source:Bioresour Technol;215:110-116, 2016 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study highlights the development of a combined pretreatment and hydrolysis strategy of rice straw for the production of bioethanol and biopolymer (poly-3-hydroxybutyrate). Maximum reducing sugar yield was 0.374g/g. The hydrolyzate is devoid of major fermentation inhibitors like furfural and organic acids and can be used for fermentation without any detoxification. Fermentation of the non-detoxified hydrolyzate with Saccharomyces cerevisiae yielded 1.48% of ethanol with a fermentation efficiency of 61.25% and with Comamonas sp. yielded 35.86% of poly-3-hydroxybutyrate without any nutrient supplementation. Characterization of native, control as well as the residue left out after combined pretreatment and hydrolysis of RS by scanning electron microscopy and X-ray diffraction showed difference. Compositional analysis revealed that the residue contains lignin and hemicellulose as the major component indicating that major portion of cellulose were hydrolyzed in this strategy.
[Mh] Termos MeSH primário: Biocombustíveis
Etanol/análise
Hidroxibutiratos/análise
Poliésteres/análise
[Mh] Termos MeSH secundário: Celulose/metabolismo
Comamonas/metabolismo
Etanol/metabolismo
Fermentação
Hidrólise
Hidroxibutiratos/metabolismo
Lignina/análise
Lignina/metabolismo
Oryza/química
Componentes Aéreos da Planta/química
Poliésteres/metabolismo
Saccharomyces cerevisiae/metabolismo
Resíduos
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Hydroxybutyrates); 0 (Polyesters); 0 (Waste Products); 26063-00-3 (poly-beta-hydroxybutyrate); 3K9958V90M (Ethanol); 9004-34-6 (Cellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE


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[PMID]:26918381
[Au] Autor:Nojiri M; Yoshida S; Kanamaru H; Yasohara Y
[Ad] Endereço:Biotechnology Development Laboratories, Kaneka Corporation, Takasago, Hyogo, Japan.
[Ti] Título:Improved efficiency of asymmetric hydrolysis of 3-substituted glutaric acid diamides with an engineered amidase.
[So] Source:J Appl Microbiol;120(6):1542-51, 2016 Jun.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To improve the efficiency of asymmetric hydrolysis of 3-(4-chlorophenyl) glutaric acid diamide (CGD) using a recombinant Comamonas sp. KNK3-7 amidase (CoAM) produced in Escherichia coli. METHODS AND RESULTS: The CoAM gene was cloned, sequenced and found to comprise 1512 bp, encoding a polypeptide of 54 054 Da. CoAM-transformed E. coli were able to perform R-selective hydrolysis of CGD; however, complete conversion of 166·2 mmol l(-1) CGD in 28 h could not be obtained. We attempted to optimize the reactivity of CoAM by mutating single amino acids in the substrate-binding domain. Notably, the methionine-substituted L146M mutant enzyme showed increased reactivity, completing the conversion of 166·2 mmol l(-1) CGD in just 4 h. The Km value for L146M was lower than that of CoAM. CONCLUSIONS: We succeeded in creating the L146M mutant of CoAM with increased substrate affinity and found that this was the best mutant for the hydrolysis of CGD. SIGNIFICANCE AND IMPACT OF THE STUDY: Increasing the efficiency of hydrolysis of 3-substituted glutaric acid diamides is useful to improve the synthesis of optically active 3-substituted gamma-aminobutyric acid. This is the first report of efficient hydrolysis of CGD using amidase mutant-producing E. coli cells.
[Mh] Termos MeSH primário: Amidoidrolases/genética
Comamonas/enzimologia
Comamonas/genética
Diamida/química
Glutaratos/química
Engenharia de Proteínas
[Mh] Termos MeSH secundário: Amidoidrolases/química
Amidoidrolases/isolamento & purificação
Sítios de Ligação
Clonagem Molecular
Comamonas/metabolismo
Escherichia coli/genética
Hidrólise
Reação em Cadeia da Polimerase
Rhodococcus/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-(4-chlorophenyl) glutaric acid diamide); 0 (Glutarates); 10465-78-8 (Diamide); EC 3.5.- (Amidohydrolases); EC 3.5.1.4 (amidase); H849F7N00B (glutaric acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13112


  9 / 173 MEDLINE  
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[PMID]:26816093
[Au] Autor:Ma J; Wang Z; Li H; Park HD; Wu Z
[Ad] Endereço:State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, 200092, People's Republic of China.
[Ti] Título:Metagenomes reveal microbial structures, functional potentials, and biofouling-related genes in a membrane bioreactor.
[So] Source:Appl Microbiol Biotechnol;100(11):5109-21, 2016 Jun.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Metagenomic sequencing was used to investigate the microbial structures, functional potentials, and biofouling-related genes in a membrane bioreactor (MBR). The results showed that the microbial community in the MBR was highly diverse. Notably, function analysis of the dominant genera indicated that common genes from different phylotypes were identified for important functional potentials with the observation of variation of abundances of genes in a certain taxon (e.g., Dechloromonas). Despite maintaining similar metabolic functional potentials with a parallel full-scale conventional activated sludge (CAS) system due to treating the identical wastewater, the MBR had more abundant nitrification-related bacteria and coding genes of ammonia monooxygenase, which could well explain its excellent ammonia removal in the low-temperature period. Furthermore, according to quantification of the genes involved in exopolysaccharide and extracellular polymeric substance (EPS) protein metabolism, the MBR did not show a much different potential in producing EPS compared to the CAS system, and bacteria from the membrane biofilm had lower abundances of genes associated with EPS biosynthesis and transport compared to the activated sludge in the MBR.
[Mh] Termos MeSH primário: Incrustação Biológica
Reatores Biológicos/microbiologia
Metagenoma
[Mh] Termos MeSH secundário: Bactérias/classificação
Bactérias/genética
Biofilmes
Comamonas/classificação
Comamonas/genética
DNA Bacteriano/genética
DNA Bacteriano/isolamento & purificação
Flavobacterium/classificação
Flavobacterium/genética
Biblioteca Gênica
Nitrificação
Polímeros/química
Pseudomonas/classificação
Pseudomonas/genética
Análise de Sequência de DNA
Esgotos/microbiologia
Thauera/classificação
Thauera/genética
Águas Residuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Polymers); 0 (Sewage); 0 (Waste Water)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160128
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7312-3


  10 / 173 MEDLINE  
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[PMID]:26587972
[Au] Autor:Shahzad A; Saddiqui S; Bano A
[Ad] Endereço:a Quaid-i-Azam University , Islamabad , Pakistan.
[Ti] Título:The response of maize (Zea mays L.) plant assisted with bacterial consortium and fertilizer under oily sludge.
[So] Source:Int J Phytoremediation;18(5):521-6, 2016.
[Is] ISSN:1549-7879
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to evaluate the role of PGPR consortium and fertilizer alone and in combination on the physiology of maize grown under oily sludge stress environment as well on the soil nutrient status. Consortium was prepared from Bacillus cereus (Acc KR232400), Bacillus altitudinis (Acc KF859970), Comamonas (Delftia) belonging to family Comamonadacea (Acc KF859971) and Stenotrophomonasmaltophilia (Acc KF859973). The experiment was conducted in pots with complete randomized design with four replicates and kept in field. Oily sludge was mixed in ml and Ammonium nitrate and Diammonium phosphate (DAP) were added at 70 ug/g and 7 ug/g at sowing. The plant was harvested at 21 d for estimation of protein, proline and antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD). To study the degradation, total petroleum hydrocarbon was extracted by soxhelt extraction and extract was analyzed by GC-FID at different period after incubation. Combined application of consortium and fertilizer enhanced the germination %, protein and, proline content by 90,130 and 99% higher than untreated maize plants. Bioavailability of macro and micro nutrient was also enhanced with consortium and fertilizer in oily sludge. The consortium and fertilizer in combined treatment decreased the superoxide dismutase (SOD), peroxidase dismutase (POD) of the maize leaves grown in oily sludge. Degradation of total petroleum hydrocarbon (TPHs) was 59% higher in combined application of consortium and fertilizer than untreated maize at 3 d. The bacterial consortium can enhanced the maize tolerance to oily sludge and enhanced degradation of total petroleum hydrocarbon (TPHs). The maize can be considered as tolerant plant species to remediate oily sludge contaminated soils.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Fertilizantes
Petróleo/metabolismo
Esgotos
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Bacillus/metabolismo
Biodegradação Ambiental
Comamonas/metabolismo
Solo
Stenotrophomonas maltophilia/metabolismo
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Petroleum); 0 (Sewage); 0 (Soil)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151121
[St] Status:MEDLINE
[do] DOI:10.1080/15226514.2015.1115964



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