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[PMID]:28629496
[Au] Autor:Carro L; Mulas R; Pastor-Bueis R; Blanco D; Terrón A; González-Andrés F; Peix A; Velázquez E
[Ad] Endereço:†â€‹Present address: School of Biology, Newcastle University, Newcastle upon Tyne, UK. 1​Departamento de Microbiología y Genética and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Salamanca, Spain.
[Ti] Título:Delftia rhizosphaerae sp. nov. isolated from the rhizosphere of Cistus ladanifer.
[So] Source:Int J Syst Evol Microbiol;67(6):1957-1960, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain, designated RA6T, was isolated from the rhizosphere of Cistus ladanifer. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate into the genus Delftia within a cluster encompassing the type strains of Delftia lacustris, Delftia tsuruhatensis, Delftia acidovorans and Delftia litopenaei, which presented greater than 97 % sequence similarity with respect to strain RA6T. DNA-DNA hybridization studies showed average relatedness ranging from of 11 to 18 % between these species of the genus Delftia and strain RA6T. Catalase and oxidase were positive. Casein was hydrolysed but gelatin and starch were not. Ubiquinone 8 was the major respiratory quinone detected in strain RA6T together with low amounts of ubiquinones 7 and 9. The major fatty acids were those from summed feature 3 (C16 : 1ω7c/C16 : 1 ω6c) and C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RA6T should be considered as a representative of a novel species of genus Delftia, for which the name Delftia rhizosphaerae sp. nov. is proposed. The type strain is RA6T (=LMG 29737T= CECT 9171T).
[Mh] Termos MeSH primário: Cistus/microbiologia
Delftia/classificação
Filogenia
Rizosfera
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Delftia/genética
Delftia/isolamento & purificação
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espanha
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8); RRK47DEG6Q (ubiquinone 7)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001892


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[PMID]:26800648
[Au] Autor:Razaif-Mazinah MRM; Anis SNS; Harun HI; Rashid KA; Annuar MSM
[Ad] Endereço:Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
[Ti] Título:Unusual poly(3-hydroxyalkanoate) (PHA) biosynthesis behavior of Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002 isolated from palm oil mill effluent.
[So] Source:Biotechnol Appl Biochem;64(2):259-269, 2017 Mar.
[Is] ISSN:1470-8744
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002, isolated from palm oil mill effluent, accumulated poly(3-hydroxyalkanoates) (PHAs) when grown on aliphatic fatty acids, sugars, and glycerol. The substrates were supplied at 20:1 C/N mole ratio. Among C-even n-alkanoic acids, myristic acid gave the highest PHA content 26 and 28 wt% in P. putida and D. tsuruhatensis, respectively. Among C-odd n-alkanoic acids, undecanoic gave the highest PHA content at 40 wt% in P. putida and 46 wt% in D. tsuruhatensis on pentadecanoic acid. Sugar and glycerol gave <10 wt% of PHA content for both bacteria. Interestingly, D. tsuruhatensis accumulated both short- and medium-chain length PHA when supplied with n-alkanoic acids ranging from octanoic to lauric, sucrose, and glycerol with 3-hydroxybutyrate as the major monomer unit. In P. putida, the major hydroxyalkanoates unit was 3-hydroxyoctanoate and 3-hydroxydecanoate when grown on C-even acids. Conversely, 3-hydroxyheptanoate, 3-hydrxoynonanoate, and 3-hydroxyundecanoate were accumulated with C-odd acids. Weight-averaged molecular weight (M ) was in the range of 53-81 kDa and 107-415 kDa for P. putida and D. tsuruhatensis, respectively. Calorimetric analyses indicated that both bacteria synthesized semicrystalline polymer with good thermal stability with degradation temperature (T ) ranging from 178 to 282 °C.
[Mh] Termos MeSH primário: Delftia/metabolismo
Óleos Vegetais/química
Poli-Hidroxialcanoatos/biossíntese
Pseudomonas putida/metabolismo
[Mh] Termos MeSH secundário: Caprilatos/química
Carbono
Delftia/química
Ácidos Graxos/química
Glicerol/química
Peso Molecular
Óleo de Palmeira
Poli-Hidroxialcanoatos/química
Pseudomonas putida/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caprylates); 0 (Fatty Acids); 0 (Plant Oils); 0 (Polyhydroxyalkanoates); 5QUO05548Z (Palm Oil); 7440-44-0 (Carbon); 8M44B02CSJ (3-hydroxyoctanoic acid); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160124
[St] Status:MEDLINE
[do] DOI:10.1002/bab.1482


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[PMID]:27847812
[Au] Autor:Guo H; Yang Y; Liu K; Xu W; Gao J; Duan H; Du B; Ding Y; Wang C
[Ad] Endereço:College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
[Ti] Título:Comparative Genomic Analysis of MTQ3 and the Identification of Functional NRPS Genes for Siderophore Production.
[So] Source:Biomed Res Int;2016:3687619, 2016.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant growth-promoting rhizobacteria (PGPR) are a group of rhizosphere bacteria that promote plant growth. MTQ3 is a member of PGPR that produces siderophores. The draft genome sequence of MTQ3 has been reported. Here, we analyzed the genome sequence of MTQ3 and performed a comparative genome analysis of four sequenced strains, revealing genetic relationships among these strains. In addition, genes responsible for bacteriocin and nonribosomal peptide synthesis were detected in the genomes of each strain. To reveal the functions of NRPS genes in siderophore production in MTQ3, three NRPS genes were knocked out to obtain the three mutants MTQ3-Δ1941, MTQ3-Δ1945, and MTQ3-Δ1946, which were compared with the wild-type strain. In qualitative and quantitative analyses using CAS assay, the mutants failed to produce siderophores. Accordingly, the NRPS genes in MTQ3 were functionally related to siderophore production. These results clarify one mechanism by which plant growth is promoted in MTQ3 and have important applications in agricultural production.
[Mh] Termos MeSH primário: Delftia/genética
Delftia/metabolismo
Genoma Bacteriano/genética
Peptídeos/metabolismo
Sideróforos/metabolismo
[Mh] Termos MeSH secundário: Técnicas de Inativação de Genes
Peptídeos/genética
Filogenia
Sideróforos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptides); 0 (Siderophores)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE


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[PMID]:27098381
[Au] Autor:Rea MA; Zammit CM; Reith F
[Ad] Endereço:School of Biological Sciences, The Sprigg Geobiology Centre, The University of Adelaide, Adelaide, South Australia 5005, Australia CSIRO Land and Water, Environmental Contaminant Mitigation and Technologies, PMB2, Glen Osmond, South Australia 5064, Australia.
[Ti] Título:Bacterial biofilms on gold grains-implications for geomicrobial transformations of gold.
[So] Source:FEMS Microbiol Ecol;92(6):fiw082, 2016 Jun.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The biogeochemical cycling of gold (Au), i.e. its solubilization, transport and re-precipitation, leading to the (trans)formation of Au grains and nuggets has been demonstrated under a range of environmental conditions. Biogenic (trans)formations of Au grains are driven by (geo)biochemical processes mediated by distinct biofilm consortia living on these grains. This review summarizes the current knowledge concerning the composition and functional capabilities of Au-grain communities, and identifies contributions of key-species involved in Au-cycling. To date, community data are available from grains collected at 10 sites in Australia, New Zealand and South America. The majority of detected operational taxonomic units detected belong to the α-, ß- and γ-Proteobacteria and the Actinobacteria. A range of organisms appears to contribute predominantly to biofilm establishment and nutrient cycling, some affect the mobilization of Au via excretion of Au-complexing ligands, e.g. organic acids, thiosulfate and cyanide, while a range of resident Proteobacteria, especially Cupriavidus metallidurans and Delftia acidovorans, have developed Au-specific biochemical responses to deal with Au-toxicity and reductively precipitate mobile Au-complexes. This leads to the biomineralization of secondary Au and drives the environmental cycle of Au.
[Mh] Termos MeSH primário: Actinobacteria/metabolismo
Alphaproteobacteria/metabolismo
Biofilmes/crescimento & desenvolvimento
Cupriavidus/metabolismo
Delftia/metabolismo
Gammaproteobacteria/metabolismo
Ouro/metabolismo
[Mh] Termos MeSH secundário: Actinobacteria/crescimento & desenvolvimento
Alphaproteobacteria/crescimento & desenvolvimento
Austrália
Cupriavidus/crescimento & desenvolvimento
Delftia/crescimento & desenvolvimento
Gammaproteobacteria/crescimento & desenvolvimento
Nova Zelândia
América do Sul
Tiossulfatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Thiosulfates); 7440-57-5 (Gold)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE


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[PMID]:26992798
[Au] Autor:Wu ZM; Zheng RC; Zheng YG
[Ad] Endereço:Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China.
[Ti] Título:Exploitation and characterization of three versatile amidase super family members from Delftia tsuruhatensis ZJB-05174.
[So] Source:Enzyme Microb Technol;86:93-102, 2016 May.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amidases can be assigned into two families according to their amino acid sequences. Three amidases (Dt-Amis) were mined and identified from genome of Delftia tsuruhatensis. Homology analysis demonstrated that Dt-Ami 2 and Dt-Ami 6 belonged to amidase signature (AS) family, while Dt-Ami 7 belonged to nitrilase superfamily. AS amidases were shown to hydrolyze a wide spectrum of amides. Kinetic analysis demonstrated that the extension of chain length of aliphatic amides considerably decreased the Km values, and the turnover numbers (kcat) were high with linear aliphatic amides as substrates. Dt-Ami 2 showed maximum activity near a quite alkaline pH (11.0) and exhibited opposite enantioselectivity to Dt-Ami 6. Furthermore, a novel bioprocess for hydrolysis of 1-cyanocyclohexaneacetamide was developed using Dt-Ami 6 as biocatalyst, resulting in >99% conversion within 1.5h at a substrate loading of 100g/L by 0.5g/L of Escherichia coli cells. On the other hand, nitrilase superfamily amidase only hydrolyzed aliphatic amides. The Km values of Dt-Ami 7 were considerably increased with the extension of chain length of aliphatic amides. The characterized enzymes from different families showed distinct biochemical characteristics and catalytic properties, leading to a better understanding of the two super amidase family members.
[Mh] Termos MeSH primário: Amidoidrolases/metabolismo
Proteínas de Bactérias/metabolismo
Delftia/enzimologia
[Mh] Termos MeSH secundário: Amidas/química
Amidas/metabolismo
Amidoidrolases/classificação
Amidoidrolases/genética
Proteínas de Bactérias/classificação
Proteínas de Bactérias/genética
Clonagem Molecular
Delftia/genética
Estabilidade Enzimática
Genes Bacterianos
Cinética
Proteínas Recombinantes/classificação
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
Estereoisomerismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amides); 0 (Bacterial Proteins); 0 (Recombinant Proteins); EC 3.5.- (Amidohydrolases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160320
[St] Status:MEDLINE


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[PMID]:26901701
[Au] Autor:Azevedo AS; Almeida C; Pereira B; Melo LF; Azevedo NF
[Ad] Endereço:a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.
[Ti] Título:Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents.
[So] Source:Biofouling;32(3):227-41, 2016.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as Delftia tsuruhatensis and Achromobacter xylosoxidans, might interact with conventional pathogenic agents such as Escherichia coli. Here, single- and dual-species biofilms of these microorganisms were characterized in terms of microbial composition over time, the average fitness of E. coli, the spatial organization and the biofilm antimicrobial profile. The results revealed a positive impact of these species on the fitness of E. coli and a greater tolerance to the antibiotic agents. In dual-species biofilms exposed to antibiotics, E. coli was able to dominate the microbial consortia in spite of being the most sensitive strain. This is the first study demonstrating the protective effect of less common species over E. coli under adverse conditions imposed by the use of antibiotic agents.
[Mh] Termos MeSH primário: Achromobacter denitrificans
Antibacterianos/farmacologia
Biofilmes
Delftia
Escherichia coli
Infecções Urinárias
[Mh] Termos MeSH secundário: Achromobacter denitrificans/efeitos dos fármacos
Achromobacter denitrificans/fisiologia
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Infecções Relacionadas a Cateter/tratamento farmacológico
Infecções Relacionadas a Cateter/microbiologia
Delftia/efeitos dos fármacos
Delftia/fisiologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/fisiologia
Seres Humanos
Interações Microbianas/efeitos dos fármacos
Interações Microbianas/fisiologia
Cateteres Urinários/efeitos adversos
Cateteres Urinários/microbiologia
Infecções Urinárias/tratamento farmacológico
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160223
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2015.1124096


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[PMID]:26607323
[Au] Autor:Herzog B; Overy DP; Haltli B; Kerr RG
[Ad] Endereço:Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI C1A 4P3, Canada; Nautilus Biosciences Canada, Duffy Research Center (NRC-INH) , 550 University Avenue, Charlottetown, PEI C1A 4P3, Canada.
[Ti] Título:Discovery of keratinases using bacteria isolated from marine environments.
[So] Source:Syst Appl Microbiol;39(1):49-57, 2016 Feb.
[Is] ISSN:1618-0984
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Bacteria are important for the biodegradation of keratin. Thus, a workflow to isolate keratin-degrading bacteria utilizing an optimized azo-keratin assay was established. Deteriorated feather samples, collected in marine shoreline environments from the intertidal zone, yielded 50 unique bacterial isolates exhibiting keratin degradation when feather meal was supplied as keratin substrate. The majority of isolates, identified by 16S sequencing, belonged to genera previously reported to produce keratinases: Bacillus spp. (42%) and Stenotrophomonas spp. (40%). The remaining 18% represented the genera Alcaligenes, Chryseobacterium, Salinivibrio, Delftia, Stappia, and Microbacterium, genera not previously been associated with keratinase production. The workflow, also applied to 21 Bacilli from our in-house culture collection, additionally revealed four Bacilli with remarkable feather degradation potential. The industrial applicability of their associated keratinases was evaluated and the most active keratinase expressed in E. coli to confirm keratinase expression. Enriched keratinase fractions demonstrated activity up to 75°C and retained viability when stored lyophilized at 20°C for up to 200d.
[Mh] Termos MeSH primário: Alcaligenes/metabolismo
Bacillus/metabolismo
Chryseobacterium/metabolismo
Delftia/metabolismo
Plumas/microbiologia
Peptídeo Hidrolases/metabolismo
Stenotrophomonas/metabolismo
[Mh] Termos MeSH secundário: Alcaligenes/isolamento & purificação
Animais
Organismos Aquáticos/isolamento & purificação
Organismos Aquáticos/metabolismo
Bacillus/isolamento & purificação
Biodegradação Ambiental
Chryseobacterium/isolamento & purificação
Delftia/isolamento & purificação
Plumas/metabolismo
Queratinas/metabolismo
Stenotrophomonas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
68238-35-7 (Keratins); EC 3.4.- (Peptide Hydrolases); EC 3.4.- (keratinase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151127
[St] Status:MEDLINE


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[PMID]:26589947
[Au] Autor:Wu W; Huang H; Ling Z; Yu Z; Jiang Y; Liu P; Li X
[Ad] Endereço:MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, People's Republic of China.
[Ti] Título:Genome sequencing reveals mechanisms for heavy metal resistance and polycyclic aromatic hydrocarbon degradation in Delftia lacustris strain LZ-C.
[So] Source:Ecotoxicology;25(1):234-47, 2016 Jan.
[Is] ISSN:1573-3017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Strain LZ-C, isolated from a petrochemical wastewater discharge site, was found to be resistant to heavy metals and to degrade various aromatic compounds, including naphenol, naphthalene, 2-methylnaphthalene and toluene. Data obtained from 16S rRNA gene sequencing showed that this strain was closely related to Delftia lacustris. The 5,889,360 bp genome of strain LZ-C was assembled into 239 contigs and 197 scaffolds containing 5855 predicted open reading frames (ORFs). Among these predicted ORFs, 464 were different from the type strain of Delftia. The minimal inhibitory concentrations were 4 mM, 30 µM, 2 mM and 1 mM for Cr(VI), Hg(II), Cd(II) and Pb(II), respectively. Both genome sequencing and quantitative real-time PCR data revealed that genes related to Chr, Czc and Mer family genes play important roles in heavy metal resistance in strain LZ-C. In addition, the Na(+)/H(+) antiporter NhaA is important for adaptation to high salinity resistance (2.5 M NaCl). The complete pathways of benzene and benzoate degradation were identified through KEGG analysis. Interestingly, strain LZ-C also degrades naphthalene but lacks the key naphthalene degradation gene NahA. Thus, we propose that strain LZ-C exhibits a novel protein with a function similar to NahA. This study is the first to reveal the mechanisms of heavy metal resistance and salinity tolerance in D. lacustris and to identify a potential 2-methylnaphthalene degradation protein in this strain. Through whole-genome sequencing analysis, strain LZ-C might be a good candidate for the bioremediation of heavy metals and polycyclic aromatic hydrocarbons.
[Mh] Termos MeSH primário: Delftia/genética
Delftia/metabolismo
Genoma Bacteriano
Metais Pesados/metabolismo
Hidrocarbonetos Aromáticos Policíclicos/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: China
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Soil Pollutants)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151122
[St] Status:MEDLINE
[do] DOI:10.1007/s10646-015-1583-9


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[PMID]:26585451
[Au] Autor:Icgen B; Yilmaz F
[Ad] Endereço:Department of Environmental Engineering, Middle East Technical University, 06800, Ankara, Turkey. bicgen@metu.edu.tr.
[Ti] Título:Design a cadA-targeted DNA probe for screening of potential bacterial cadmium biosorbents.
[So] Source:Environ Sci Pollut Res Int;23(6):5743-52, 2016 Mar.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Due to their metal removal ability, bacterial biosorbents can be effectively used for the treatment of wastewaters containing heavy metals. Searching for bacterial biosorbents for hazardous heavy metals like cadmium is a pivotal for remediation efforts. The gene cadA, that mediates resistance to cadmium over an ATP-dependent efflux mechanism, provides a good target for the selection of potential cadmium biosorbents. For this reason, in this study, a 36-mer-oligonucleotide DNA probe based on the entire 3.5-kb BglII-XbaI fragment of cadA operon from staphylococcal plasmid pI258 was prepared by using Vector NTI Express software. Under the hybridization conditions of 46 °C, 50 % formamide, and 0.028 M NaCl, the designed cadA probe appeared to be highly specific to the cadA-positive Staphylococcus warneri and Delftia acidovorans isolates tested. The results indicated that the newly designed cadA-targeted DNA probe has potential as a specific, sensitive, and quantitative tool in selecting and in situ screening of potential cadmium biosorbents.
[Mh] Termos MeSH primário: Cádmio/análise
Sondas de DNA
Genes Bacterianos
Águas Residuais/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Sondas de DNA/genética
Delftia/genética
Delftia/crescimento & desenvolvimento
Fluoresceína-5-Isotiocianato/química
Plasmídeos
Staphylococcus/genética
Staphylococcus/crescimento & desenvolvimento
Águas Residuais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Probes); 0 (Waste Water); 0 (Water Pollutants, Chemical); 00BH33GNGH (Cadmium); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151121
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-015-5810-y


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[PMID]:26386060
[Au] Autor:Dong W; Chen Q; Hou Y; Li S; Zhuang K; Huang F; Zhou J; Li Z; Wang J; Fu L; Zhang Z; Huang Y; Wang F; Cui Z
[Ad] Endereço:Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, People's Republic of China.
[Ti] Título:Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and cloning of the novel flavin-dependent monooxygenase system meaBA.
[So] Source:Appl Environ Microbiol;81(24):8254-64, 2015 Dec.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant strain MEA3-1Mut, which converts MEA only to 2-methyl-6-ethyl-hydroquinone (MEHQ) and 2-methyl-6-ethyl-benzoquinone (MEBQ). MEA may be oxidized by the P450 monooxygenase system to 4-hydroxy-2-methyl-6-ethylaniline (4-OH-MEA), which can be hydrolytically spontaneously deaminated to MEBQ or MEHQ. The MEA microbial metabolic pathway was reconstituted based on the substrate spectra and identification of the intermediate metabolites in both the wild-type and mutant strains. Plasmidome sequencing indicated that both strains harbored 7 plasmids with sizes ranging from 6,108 bp to 287,745 bp. Among the 7 plasmids, 6 were identical, and pMEA02' in strain MEA3-1Mut lost a 37,000-bp fragment compared to pMEA02 in strain MEA3-1. Two-dimensional electrophoresis (2-DE) and protein mass fingerprinting (PMF) showed that MEA3-1Mut lost the two-component flavin-dependent monooxygenase (TC-FDM) MeaBA, which was encoded by a gene in the lost fragment of pMEA02. MeaA shared 22% to 25% amino acid sequence identity with oxygenase components of some TC-FDMs, whereas MeaB showed no sequence identity with the reductase components of those TC-FDMs. Complementation with meaBA in MEA3-1Mut and heterologous expression in Pseudomonas putida strain KT2440 resulted in the production of an active MEHQ monooxygenase.
[Mh] Termos MeSH primário: Delftia/metabolismo
Oxigenases/genética
Sphingomonadaceae/metabolismo
Toluidinas/metabolismo
[Mh] Termos MeSH secundário: Acetamidas/metabolismo
Sequência de Aminoácidos
Sequência de Bases
Biodegradação Ambiental
DNA Bacteriano/genética
Delftia/enzimologia
Delftia/genética
Eletroforese em Gel Bidimensional
Redes e Vias Metabólicas/genética
Dados de Sequência Molecular
Oxigenases/metabolismo
Mapeamento de Peptídeos
Pseudomonas putida/metabolismo
Análise de Sequência de DNA
Sphingomonadaceae/enzimologia
Sphingomonadaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-methyl-6-ethylaniline); 0 (Acetamides); 0 (DNA, Bacterial); 0 (Toluidines); 78194-29-3 (2-chloro-N-(ethoxymethyl)-N-(2-methyl-6-(trifluoromethyl)phenyl)acetamide); EC 1.13.- (Oxygenases); EC 1.14.13.8 (dimethylaniline monooxygenase (N-oxide forming))
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150920
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01883-15



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