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Pesquisa : B03.440.400.425.297 [Categoria DeCS]
Referências encontradas : 29 [refinar]
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  1 / 29 MEDLINE  
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[PMID]:27514019
[Au] Autor:Leclair M; Polin S; Jousseaume T; Simon JC; Sugio A; Morlière S; Fukatsu T; Tsuchida T; Outreman Y
[Ad] Endereço:IGEPP, Agrocampus Ouest, INRA, Université de Rennes 1, Université Bretagne-Loire, Rennes, France.
[Ti] Título:Consequences of coinfection with protective symbionts on the host phenotype and symbiont titres in the pea aphid system.
[So] Source:Insect Sci;24(5):798-808, 2017 Oct.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Symbiotic associations between microbes and insects are widespread, and it is frequent that several symbionts share the same host individual. Hence, interactions can occur between these symbionts, influencing their respective abundance within the host with consequences on its phenotype. Here, we investigate the effects of multiple infections in the pea aphid, Acyrthosiphon pisum, which is the host of an obligatory and several facultative symbionts. In particular, we study the influence of a coinfection with 2 protective symbionts: Hamiltonella defensa, which confers protection against parasitoids, and Rickettsiella viridis, which provides protection against fungal pathogens and predators. The effects of Hamiltonella-Rickettsiella coinfection on the respective abundance of the symbionts, host fitness and efficacy of enemy protection were studied. Asymmetrical interactions between the 2 protective symbionts have been found: when they coinfect the same aphid individuals, the Rickettsiella infection affected Hamiltonella abundance within hosts but not the Hamiltonella-mediated protective phenotype while the Hamiltonella infection negatively influences the Rickettsiella-mediated protective phenotype but not its abundance. Harboring the 2 protective symbionts also reduced the survival and fecundity of host individuals. Overall, this work highlights the effects of multiple infections on symbiont abundances and host traits that are likely to impact the maintenance of the symbiotic associations in natural habitats.
[Mh] Termos MeSH primário: Afídeos/microbiologia
Coxiellaceae/fisiologia
Enterobacteriaceae/fisiologia
Interações Hospedeiro-Parasita
Simbiose
Vespas/fisiologia
[Mh] Termos MeSH secundário: Animais
Afídeos/genética
Afídeos/parasitologia
Coinfecção
Feminino
Masculino
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12380


  2 / 29 MEDLINE  
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[PMID]:26573831
[Au] Autor:Duron O; Cremaschi J; McCoy KD
[Ad] Endereço:Laboratoire MIVEGEC (Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle), Centre National de la Recherche Scientifique (UMR5290)-Université de Montpellier-Institut pour la Recherche et le Développement (UR 224), Montpellier, France. olivier.duron@ird.fr.
[Ti] Título:The High Diversity and Global Distribution of the Intracellular Bacterium Rickettsiella in the Polar Seabird Tick Ixodes uriae.
[So] Source:Microb Ecol;71(3):761-70, 2016 Apr.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Obligate intracellular bacteria of the Rickettsiella genus are emerging as both widespread and biologically diverse in arthropods. Some Rickettsiella strains are highly virulent entomopathogenic agents, whereas others are maternally inherited endosymbionts exerting very subtle manipulations on host phenotype to promote their own spread. Recently, a variety of Rickettsiella strains have been reported from ticks, but their biology is entirely unknown. In the present study, we examined the incidence and diversity of Rickettsiella in 11 geographically distinct populations of the polar seabird tick Ixodes uriae. We found Rickettsiella in most tick populations with a prevalence ranging from 3 to 24 %. 16S ribosomal RNA (rRNA) and GroEL gene sequences revealed an unexpected diversity of Rickettsiella, with 12 genetically distinct Rickettsiella strains present in populations of I. uriae. Phylogenetic investigations further revealed that these Rickettsiella strains do not cluster within a tick-specific clade but rather exhibit distinct evolutionary origins demonstrating frequent horizontal transfers between distantly related arthropod species. Tick rearing further showed that Rickettsiella are present in eggs laid by infected females with no evidence of abortive development. Using this data set, we discuss the potential biological significance of Rickettsiella in seabird ticks. Most notably, we suggest that these organisms may not be pathogenic forms but rather use more subtle adaptive strategies to persist within tick populations.
[Mh] Termos MeSH primário: Biodiversidade
Doenças das Aves/microbiologia
Doenças das Aves/parasitologia
Coxiellaceae/isolamento & purificação
Ixodes/microbiologia
[Mh] Termos MeSH secundário: Animais
Aves/microbiologia
Aves/parasitologia
Coxiellaceae/classificação
Coxiellaceae/genética
Feminino
Masculino
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151118
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-015-0702-8


  3 / 29 MEDLINE  
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[PMID]:26269380
[Au] Autor:Duron O
[Ad] Endereço:Laboratoire MIVEGEC (Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle), Centre National de la Recherche Scientifique (UMR5290) - Université de Montpellier - Institut pour la Recherche et le Développement (UR224), F-34394 Montpellier, France olivier.duron@ird.fr.
[Ti] Título:The IS1111 insertion sequence used for detection of Coxiella burnetii is widespread in Coxiella-like endosymbionts of ticks.
[So] Source:FEMS Microbiol Lett;362(17):fnv132, 2015 Sep.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Coxiella is a genus of obligate intracellular bacteria engaged in a variety of interactions with eukaryotes. The type species, Coxiella burnetii, infects several vertebrate species, including humans, and is the causative agent of Q fever. Multiple copies of a specific transposable element, the insertion sequence IS1111, are present in the genome of C. burnetii and are routinely used for confirmation of Q fever cases. Recently, many Coxiella-like bacteria that are closely related but genetically distinct to C. burnetii have been found in ticks. These Coxiella-like bacteria are maternally inherited endosymbionts, present at high prevalence in tick populations and engaged in mutualistic interactions with their arthropod hosts. In this study, the presence of IS1111 was examined in the Coxiella-like endosymbionts and in bacteria of the Coxiella sister-genus, Rickettsiella. This screening reveals that a wide range of IS1111 copies were present in the Coxiella-like endosymbionts of ticks. DNA sequencing further identified genetically divergent IS1111 copies, including degraded copies that constitute an important genomic fossil record of past IS1111 expansions. These results show that IS1111 is not specific to C. burnetii, suggesting that Q fever detection assays based only on this element may lead to misidentification with Coxiella-like endosymbionts.
[Mh] Termos MeSH primário: Argasidae/microbiologia
Coxiella burnetii/genética
Coxiella burnetii/isolamento & purificação
Coxiella/genética
Elementos de DNA Transponíveis
Ixodidae/microbiologia
Simbiose
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Coxiellaceae/genética
DNA Bacteriano/isolamento & purificação
Genoma Bacteriano
Seres Humanos
Febre Q/diagnóstico
Febre Q/microbiologia
Alinhamento de Sequência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150828
[Lr] Data última revisão:
150828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150814
[St] Status:MEDLINE


  4 / 29 MEDLINE  
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[PMID]:25978383
[Au] Autor:Duron O; Noël V; McCoy KD; Bonazzi M; Sidi-Boumedine K; Morel O; Vavre F; Zenner L; Jourdain E; Durand P; Arnathau C; Renaud F; Trape JF; Biguezoton AS; Cremaschi J; Dietrich M; Léger E; Appelgren A; Dupraz M; Gómez-Díaz E; Diatta G; Dayo GK; Adakal H; Zoungrana S; Vial L; Chevillon C
[Ad] Endereço:Laboratoire MIVEGEC (Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle), Centre National de la Recherche Scientifique (UMR5290)-Université de Montpellier-Institut pour la Recherche et le Développement (UR 224), Montpellier, France.
[Ti] Título:The Recent Evolution of a Maternally-Inherited Endosymbiont of Ticks Led to the Emergence of the Q Fever Pathogen, Coxiella burnetii.
[So] Source:PLoS Pathog;11(5):e1004892, 2015 May.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Q fever is a highly infectious disease with a worldwide distribution. Its causative agent, the intracellular bacterium Coxiella burnetii, infects a variety of vertebrate species, including humans. Its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. A few tick species were recently found to harbor maternally-inherited Coxiella-like organisms engaged in symbiotic interactions, but their relationships to the Q fever pathogen remain unclear. Here, we extensively sampled ticks, identifying new and atypical Coxiella strains from 40 of 58 examined species, and used this data to infer the evolutionary processes leading to the emergence of C. burnetii. Phylogenetic analyses of multi-locus typing and whole-genome sequencing data revealed that Coxiella-like organisms represent an ancient and monophyletic group allied to ticks. Remarkably, all known C. burnetii strains originate within this group and are the descendants of a Coxiella-like progenitor hosted by ticks. Using both colony-reared and field-collected gravid females, we further establish the presence of highly efficient maternal transmission of these Coxiella-like organisms in four examined tick species, a pattern coherent with an endosymbiotic lifestyle. Our laboratory culture assays also showed that these Coxiella-like organisms were not amenable to culture in the vertebrate cell environment, suggesting different metabolic requirements compared to C. burnetii. Altogether, this corpus of data demonstrates that C. burnetii recently evolved from an inherited symbiont of ticks which succeeded in infecting vertebrate cells, likely by the acquisition of novel virulence factors.
[Mh] Termos MeSH primário: Evolução Biológica
Doenças Transmissíveis Emergentes/transmissão
Coxiella burnetii/fisiologia
Saúde Global
Febre Q/transmissão
Simbiose
Carrapatos/microbiologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Comportamento Animal
Linhagem Celular
Doenças Transmissíveis Emergentes/epidemiologia
Doenças Transmissíveis Emergentes/microbiologia
Doenças Transmissíveis Emergentes/veterinária
Coxiella burnetii/classificação
Coxiella burnetii/crescimento & desenvolvimento
Coxiella burnetii/isolamento & purificação
Coxiellaceae/classificação
Coxiellaceae/crescimento & desenvolvimento
Coxiellaceae/isolamento & purificação
Coxiellaceae/fisiologia
Feminino
Genoma Bacteriano
Seres Humanos
Masculino
Troca Materno-Fetal
Viabilidade Microbiana
Dados de Sequência Molecular
Filogenia
Gravidez
Prevalência
Febre Q/epidemiologia
Febre Q/microbiologia
Febre Q/veterinária
Carrapatos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150528
[Lr] Data última revisão:
150528
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1004892


  5 / 29 MEDLINE  
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[PMID]:24880712
[Au] Autor:Kleespies RG; Federici BA; Leclerque A
[Ad] Endereço:Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstraße 243, 64287 Darmstadt, Germany.
[Ti] Título:Ultrastructural characterization and multilocus sequence analysis (MLSA) of 'Candidatus Rickettsiella isopodorum', a new lineage of intracellular bacteria infecting woodlice (Crustacea: Isopoda).
[So] Source:Syst Appl Microbiol;37(5):351-9, 2014 Jul.
[Is] ISSN:1618-0984
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The taxonomic genus Rickettsiella (Gammaproteobacteria; Legionellales) comprises intracellular bacteria associated with a wide range of arthropods including insects, arachnids and crustaceans. The present study provides ultrastructural together with genetic evidence for a Rickettsiella bacterium in the common rough woodlouse, Porcellio scaber (Isopoda, Porcellionidae), occurring in Germany, and shows that this bacterium is very closely related to one of the same genus occurring in California that infects the pill bug, Armadillidium vulgare (Isopoda, Armadillidiidae). Both bacterial isolates displayed the ultrastructural features described previously for crustacean-associated bacteria of the genus Rickettsiella, including the absence of well-defined associated protein crystals; occurrence of the latter is a typical characteristic of infection by this type of bacteria in insects, but has not been reported in crustaceans. A molecular systematic approach combining multilocus sequence analysis (MLSA) with likelihood-based significance testing demonstrated that despite their distant geographic origins, both bacteria form a tight sub-clade within the genus Rickettsiella. In the 16S rRNA gene trees, this sub-clade includes other bacterial sequences from woodlice. Moreover, the bacterial specimens from P. scaber and A. vulgare are found genetically or morphologically different from each of the four currently recognized Rickettsiella species. Therefore, the designation 'Candidatus Rickettsiella isopodorum' is introduced for this new lineage of isopod-associated Rickettsiella bacteria.
[Mh] Termos MeSH primário: Coxiellaceae/classificação
Coxiellaceae/ultraestrutura
Isópodes/microbiologia
Microscopia Eletrônica de Transmissão
Tipagem de Sequências Multilocus
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Coxiellaceae/genética
Coxiellaceae/isolamento & purificação
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Alemanha
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:140705
[Lr] Data última revisão:
140705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140602
[St] Status:MEDLINE


  6 / 29 MEDLINE  
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[PMID]:24334664
[Au] Autor:Anstead CA; Chilton NB
[Ad] Endereço:Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
[Ti] Título:Discovery of novel Rickettsiella spp. in ixodid ticks from Western Canada.
[So] Source:Appl Environ Microbiol;80(4):1403-10, 2014 Feb.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genomic DNA from four species of ixodid ticks in western Canada was tested for the presence of Rickettsiella by PCR analyses targeting the 16S rRNA gene. Eighty-eight percent of the Ixodes angustus (n = 270), 43% of the I. sculptus (n = 61), and 4% of the I. kingi (n = 93) individuals examined were PCR positive for Rickettsiella, whereas there was no evidence for the presence of Rickettsiella in Dermacentor andersoni (n = 45). Three different single-strand conformation polymorphism profiles of the 16S rRNA gene were detected among amplicons derived from Rickettsiella-positive ticks, each corresponding to a different sequence type. Furthermore, each sequence type was associated with a different tick species. Phylogenetic analyses of sequence data of the 16S rRNA gene and three other genes (rpsA, gidA, and sucB) revealed that all three sequence types were placed in a clade that contained species and pathotypes of the genus Rickettsiella. The bacterium in I. kingi represented the sister taxon to the Rickettsiella in I. sculptus, and both formed a clade with Rickettsiella grylli from crickets (Gryllus bimaculatus) and "R. ixodidis" from I. woodi. In contrast, the Rickettsiella in I. angustus was not a member of this clade but was placed external to the clade comprising the pathotypes of R. popilliae. The results indicate the existence of at least two new species of Rickettsiella: one in I. angustus and another in I. kingi and I. sculptus. However, the Rickettsiella strains in I. kingi and I. sculptus may also represent different species because each had unique sequences for all four genes.
[Mh] Termos MeSH primário: Coxiellaceae/classificação
Coxiellaceae/isolamento & purificação
Ixodes/microbiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Canadá
Análise por Conglomerados
Coxiellaceae/genética
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Dermacentor/microbiologia
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo Conformacional de Fita Simples
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131217
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.03564-13


  7 / 29 MEDLINE  
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[PMID]:24212575
[Au] Autor:Tsuchida T; Koga R; Fujiwara A; Fukatsu T
[Ad] Endereço:Frontier Research Core for Life Sciences, University of Toyama, Gofuku, Toyama, Japan.
[Ti] Título:Phenotypic effect of "Candidatus Rickettsiella viridis," a facultative symbiont of the pea aphid (Acyrthosiphon pisum), and its interaction with a coexisting symbiont.
[So] Source:Appl Environ Microbiol;80(2):525-33, 2014 Jan.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A gammaproteobacterial facultative symbiont of the genus Rickettsiella was recently identified in the pea aphid, Acyrthosiphon pisum. Infection with this symbiont altered the color of the aphid body from red to green, potentially affecting the host's ecological characteristics, such as attractiveness to different natural enemies. In European populations of A. pisum, the majority of Rickettsiella-infected aphids also harbor another facultative symbiont, of the genus Hamiltonella. We investigated this Rickettsiella symbiont for its interactions with the coinfecting Hamiltonella symbiont, its phenotypic effects on A. pisum with and without Hamiltonella coinfection, and its infection prevalence in A. pisum populations. Histological analyses revealed that coinfecting Rickettsiella and Hamiltonella exhibited overlapping localizations in secondary bacteriocytes, sheath cells, and hemolymph, while Rickettsiella-specific localization was found in oenocytes. Rickettsiella infections consistently altered hosts' body color from red to green, where the greenish hue was affected by both host and symbiont genotypes. Rickettsiella-Hamiltonella coinfections also changed red aphids to green; this greenish hue tended to be enhanced by Hamiltonella coinfection. With different host genotypes, Rickettsiella infection exhibited either weakly beneficial or nearly neutral effects on host fitness, whereas Hamiltonella infection and Rickettsiella-Hamiltonella coinfection had negative effects. Despite considerable frequencies of Rickettsiella infection in European and North American A. pisum populations, no Rickettsiella infection was detected among 1,093 insects collected from 14 sites in Japan. On the basis of these results, we discuss possible mechanisms for the interaction of Rickettsiella with other facultative symbionts, their effects on their hosts' phenotypes, and their persistence in natural host populations. We propose the designation "Candidatus Rickettsiella viridis" for the symbiont.
[Mh] Termos MeSH primário: Afídeos/microbiologia
Coxiellaceae/fisiologia
Simbiose
[Mh] Termos MeSH secundário: Animais
Afídeos/fisiologia
Coxiellaceae/genética
Japão
Dados de Sequência Molecular
Fenótipo
Pigmentação
Proteobactérias/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1410
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131112
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.03049-13


  8 / 29 MEDLINE  
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[PMID]:23645190
[Au] Autor:Iasur-Kruh L; Weintraub PG; Mozes-Daube N; Robinson WE; Perlman SJ; Zchori-Fein E
[Ad] Endereço:Department of Entomology, Newe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay, Israel.
[Ti] Título:Novel Rickettsiella bacterium in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae).
[So] Source:Appl Environ Microbiol;79(14):4246-52, 2013 Jul.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria in the genus Rickettsiella (Coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. The current report characterizes a novel Rickettsiella found in the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae), a major vector of phytoplasma diseases in Europe and Asia. Denaturing gradient gel electrophoresis (DGGE) and pyrosequencing were used to survey the main symbionts of O. albicinctus, revealing the obligate symbionts Sulcia and Nasuia, and the facultative symbionts Arsenophonus and Wolbachia, in addition to Rickettsiella. The leafhopper Rickettsiella is allied with bacteria found in ticks. Screening O. albicinctus from the field showed that Rickettsiella is highly prevalent, with over 60% of individuals infected. A stable Rickettsiella infection was maintained in a leafhopper laboratory colony for at least 10 generations, and fluorescence microscopy localized bacteria to accessory glands of the female reproductive tract, suggesting that the bacterium is vertically transmitted. Future studies will be needed to examine how Rickettsiella affects host fitess and its ability to vector phytopathogens.
[Mh] Termos MeSH primário: Coxiellaceae/classificação
Coxiellaceae/isolamento & purificação
Hemípteros/microbiologia
Hemípteros/fisiologia
[Mh] Termos MeSH secundário: Animais
Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/fisiologia
Coxiellaceae/genética
Coxiellaceae/fisiologia
DNA Bacteriano/genética
Eletroforese em Gel de Gradiente Desnaturante
Enterobacteriaceae/classificação
Enterobacteriaceae/genética
Enterobacteriaceae/fisiologia
Feminino
Hibridização in Situ Fluorescente
Israel
Masculino
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
Alinhamento de Sequência
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130507
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.00721-13


  9 / 29 MEDLINE  
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[PMID]:23007524
[Au] Autor:Schuster C; Kleespies RG; Ritter C; Feiertag S; Leclerque A
[Ad] Endereço:Institut für Biologischen Pflanzenschutz, Julius Kühn-Institut, Darmstadt, Germany.
[Ti] Título:Multilocus sequence analysis (MLSA) of 'Rickettsiella agriotidis', an intracellular bacterial pathogen of Agriotes wireworms.
[So] Source:Curr Microbiol;66(1):1-9, 2013 Jan.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wireworms, the polyphagous larvae of click beetles belonging to the genus Agriotes (Coleoptera: Elateridae) are severe and widespread agricultural pests that affect numerous crops globally. A new bacterial specimen identified in diseased wireworms had previously been shown by microscopy and 16S ribosomal RNA (rRNA) gene-based phylogenetic reconstruction to belong to the taxonomic genus Rickettsiella (Gammaproteobacteria) that comprises intracellular bacteria associated with and typically pathogenic for a wide range of arthropods. Going beyond these earlier results obtained from rRNA phylogenies, multilocus sequence analysis (MLSA) using a four marker scheme has been employed in the molecular taxonomic characterization of the new Rickettsiella pathotype, referred to as 'Rickettsiella agriotidis'. In combination with likelihood-based significance testing, the MLSA approach demonstrated the close phylogenetic relationship of 'R. agriotidis' to the pathotypes 'Rickettsiella melolonthae' and 'Rickettsiella tipulae', i.e., subjective synonyms of the nomenclatural type species, Rickettsiella popilliae. 'R. agriotidis' forms, therefore, part of a Rickettsiella pathotype complex that most likely represents the species R. popilliae. As there are currently no genetic data available from the R. popilliae type strain, the respective assignment cannot be corroborated directly. However, an alternative taxonomic assignment to the species Rickettsiella grylli has been positively ruled out by significance testing. MLSA has been shown to provide a more powerful tool for taxonomic delineation within the genus Rickettsiella as compared to 16S rRNA phylogenetics. However, the limitations of the present MLSA scheme for the sub-species level classification of 'R. agriotidis' and further R. popilliae synonyms has been critically evaluated.
[Mh] Termos MeSH primário: Coleópteros/microbiologia
Coxiellaceae/classificação
Coxiellaceae/genética
Tipagem de Sequências Multilocus
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Coxiellaceae/isolamento & purificação
DNA Bacteriano/genética
Genótipo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120926
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-012-0219-z


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[PMID]:22856625
[Au] Autor:Leclerque A; Kleespies RG; Schuster C; Richards NK; Marshall SD; Jackson TA
[Ad] Endereço:Julius Kühn-Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biological Control, Darmstadt, Germany. leclerque@hotmail.com
[Ti] Título:Multilocus sequence analysis (MLSA) of 'Rickettsiella costelytrae' and 'Rickettsiella pyronotae', intracellular bacterial entomopathogens from New Zealand.
[So] Source:J Appl Microbiol;113(5):1228-37, 2012 Nov.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Larvae of scarab beetles live in the soil and are frequently hosts for microbial pathogens. In New Zealand, larvae of the grass grub, Costelytrae zealandica (Coleoptera: Scarabaeidae), and manuka beetles, Pyronota spp. (Coleoptera: Scarabaeidae), have been collected from field populations showing loss of vigour and a whitened appearance. Diagnosis indicated an intracellular infection of fat body tissues by Rickettsiella-like micro-organisms. Rickettsiella bacteria are under evaluation as a possible new source of insect bio-control agents for important agricultural pests as, e.g. scarabaeid and elaterid larvae. The present study aimed at the unequivocal molecular taxonomic identification and comparison of the bacteria associated with Costelytra and Pyronota. METHODS AND RESULTS: Electron microscopy and phylogenetic reconstruction using a multilocus sequence analysis approach based on the 16S ribosomal RNA gene together with four protein-encoding markers (ftsY, gidA, rpsA, and sucB) demonstrated that both bacteria from New Zealand are phylogenetically closely related, but not identical, and belong to the taxonomic genus Rickettsiella. CONCLUSIONS: The bacteria under study should be referred to as pathotypes 'Rickettsiella costelytrae' and 'Rickettsiella pyronotae', respectively. Moreover, on the basis of the currently accepted systematic organization of the genus Rickettsiella, both pathotypes should be considered synonyms of the nomenclatural type species, Rickettsiella popilliae. SIGNIFICANCE AND IMPACT OF THE STUDY: The study demonstrates that Rickettsiella bacteria are geographically widespread pathogens of scarabaeid larvae. Implications of the phylogenetic findings presented for the stability of host adaptation by Rickettsiella bacteria are critically discussed.
[Mh] Termos MeSH primário: Coleópteros/microbiologia
Coxiellaceae/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Coxiellaceae/genética
DNA Bacteriano/genética
Genes Bacterianos
Larva/microbiologia
Funções Verossimilhança
Tipagem de Sequências Multilocus
Nova Zelândia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120804
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2672.2012.05419.x



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