Base de dados : MEDLINE
Pesquisa : B03.440.400.425.297.150 [Categoria DeCS]
Referências encontradas : 841 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 85 ir para página                         

  1 / 841 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28328183
[Au] Autor:Messinger CJ; Gurzau ES; Breitschwerdt EB; Tomuleasa CI; Trufan SJ; Flonta MM; Maggi RG; Berindan-Neagoe I; Rabinowitz PM
[Ad] Endereço:Yale University, New Haven, CT, USA.
[Ti] Título:Seroprevalence of Bartonella species, Coxiella burnetii and Toxoplasma gondii among patients with hematological malignancies: A pilot study in Romania.
[So] Source:Zoonoses Public Health;64(6):485-490, 2017 Sep.
[Is] ISSN:1863-2378
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Patients receiving immunosuppressive cancer treatments in settings where there is a high degree of human-animal interaction may be at increased risk for opportunistic zoonotic infections or reactivation of latent infections. We sought to determine the seroprevalence of selected zoonotic pathogens among patients diagnosed with haematologic malignancies and undergoing chemotherapeutic treatments in Romania, where much of the general population lives and/or works in contact with livestock. A convenience sample of 51 patients with haematologic cancer undergoing chemotherapy at a referral clinic in Cluj-Napoca, Romania, was surveyed regarding animal exposures. Blood samples were obtained and tested for evidence of infection with Bartonella species, Coxiella burnetii and Toxoplasma gondii, which are important opportunistic zoonotic agents in immunocompromised individuals. 58.8% of participants reported living or working on a farm, and living or working on a farm was associated with contact with livestock and other animals. 37.5% of participants were IgG seroreactive against one or more of five Bartonella antigens, and seroreactivity was statistically associated with living on farms. Farm dwellers were 3.6 times more likely to test IgG seroreactive to Bartonella antibodies than non-farm dwellers. 47.1% of the participants tested T. gondii IgG positive and 13.7% tested C. burnetii IgG positive, indicating past or latent infection. C. burnetii IgM antibodies were detected in four participants (7.8%), indicating possible recent infection. These results indicate that a large proportion of patients with haematologic cancer in Romania may be at risk for zoonotic infections or for reactivation of latent zoonotic infections, particularly with respect to Bartonella species. Special attention should be paid to cancer patients' exposure to livestock and companion animals in areas where much of the population lives in rural settings.
[Mh] Termos MeSH primário: Infecções por Bartonella/complicações
Leucemia/complicações
Febre Q/complicações
Estudos Soroepidemiológicos
Toxoplasmose/complicações
[Mh] Termos MeSH secundário: Adulto
Animais
Bartonella/isolamento & purificação
Infecções por Bartonella/epidemiologia
Coxiella/isolamento & purificação
Feminino
Seres Humanos
Leucemia/epidemiologia
Masculino
Meia-Idade
Febre Q/epidemiologia
Fatores de Risco
Romênia/epidemiologia
Toxoplasma/isolamento & purificação
Toxoplasmose/epidemiologia
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1111/zph.12353


  2 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28284601
[Au] Autor:Oskam CL; Gofton AW; Greay TL; Yang R; Doggett S; Ryan UM; Irwin PJ
[Ad] Endereço:Vector and Water-Borne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, 90 South Street, Murdoch, Perth, Western Australia, 6150, Australia. Electronic address: c.oskam@murdoch.edu.au.
[Ti] Título:Molecular investigation into the presence of a Coxiella sp. in Rhipicephalus sanguineus ticks in Australia.
[So] Source:Vet Microbiol;201:141-145, 2017 Mar.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Q fever is an infectious disease with a global distribution caused by the intracellular bacterium, Coxiella burnetii, which has been detected in a large number of tick species worldwide, including the brown dog tick, Rhipicephalus sanguineus. Recent reports of a high seroprevalance of C. burnetii in Australian dogs, along with the identification of additional Coxiella species within R. sanguineus ticks, has prompted an investigation into the presence and identification of Coxiella species in R. sanguineus ticks in Australia. Using a combination of C. burnetii species-specific IS1111a transposase gene and Coxiella genus-specific 16S rRNA PCR assays, a Coxiella sp. was identified in 100% (n=199) of R. sanguineus ticks analysed, and C. burnetii was not detected in any R. sanguineus ticks studied. Phylogenetic analysis of the 16S rRNA gene revealed the Coxiella sequences were closely related to Coxiella sp. identified previously in R. sanguineus and R. turanicus ticks overseas. This study illustrates the value of using genus specific PCR assays to detect previously unreported bacterial species. Furthermore, the presence of an additional Coxiella sp. in Australia requires further investigation into its potential for contributing to serological cross-reactions during Q fever testing.
[Mh] Termos MeSH primário: Vetores Aracnídeos/microbiologia
Coxiella/isolamento & purificação
Doenças do Cão/epidemiologia
Infecções por Bactérias Gram-Negativas/veterinária
Rhipicephalus sanguineus/microbiologia
[Mh] Termos MeSH secundário: Animais
Austrália/epidemiologia
Coxiella/genética
Coxiella burnetii/genética
Coxiella burnetii/isolamento & purificação
DNA Ribossômico/química
DNA Ribossômico/genética
Doenças do Cão/microbiologia
Cães
Feminino
Infecções por Bactérias Gram-Negativas/epidemiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Seres Humanos
Masculino
Filogenia
Febre Q/epidemiologia
Febre Q/microbiologia
Febre Q/veterinária
Análise de Sequência de DNA/veterinária
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  3 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28193163
[Au] Autor:Faruque LI; Zaman RU; Gurley ES; Massung RF; Alamgir AS; Galloway RL; Powers AM; Bai Y; Kosoy M; Nicholson WL; Rahman M; Luby SP
[Ad] Endereço:International Centre for Diarrheal Diseases Research, Bangladesh (icddr,b), Dhaka, Bangladesh. labibimran@yahoo.com.
[Ti] Título:Prevalence and clinical presentation of Rickettsia, Coxiella, Leptospira, Bartonella and chikungunya virus infections among hospital-based febrile patients from December 2008 to November 2009 in Bangladesh.
[So] Source:BMC Infect Dis;17(1):141, 2017 Feb 13.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We conducted a study to identify Rickettsia, Coxiella, Leptospira, Bartonella, and Chikungunya virus infections among febrile patients presenting at hospitals in Bangladesh. METHODS: We collected blood samples from patients at six tertiary hospitals from December 2008 to November 2009 and performed laboratory tests at the United States Centers for Disease Control and Prevention (CDC). RESULTS: Out of 720 enrolled patients, 263 (37%) were infected with Rickettsia; 132 patients had immunofluorescence antibody titer >64 against spotted fever, 63 patients against scrub typhus fever and 10 patients against typhus fever. Ten patients were identified with Coxiella. We isolated Leptospira from two patients and Bartonella from one patient. Ten patients had antibodies against Chikungunya virus. The proportion of patients who died was higher with rickettsial fever (5%) compared to those without a diagnosis of rickettsial infection (2%). None of the patients were initially diagnosed with rickettsial fever. CONCLUSIONS: Rickettsial infections are frequent yet under-recognized cause of febrile illness in Bangladesh. Clinical guidelines should be revised so that local clinicians can diagnose rickettsial infections and provide appropriate drug treatment.
[Mh] Termos MeSH primário: Febre de Chikungunya/virologia
Febre/microbiologia
Técnica Indireta de Fluorescência para Anticorpo
Infecções por Bactérias Gram-Negativas/microbiologia
Pacientes Internados/estatística & dados numéricos
Tifo por Ácaros/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Anticorpos Antibacterianos/sangue
Bangladesh/epidemiologia
Bartonella/isolamento & purificação
Centers for Disease Control and Prevention (U.S.)
Febre de Chikungunya/epidemiologia
Febre de Chikungunya/imunologia
Criança
Pré-Escolar
Coxiella/isolamento & purificação
Feminino
Febre/epidemiologia
Infecções por Bactérias Gram-Negativas/epidemiologia
Infecções por Bactérias Gram-Negativas/imunologia
Seres Humanos
Lactente
Recém-Nascido
Leptospira/isolamento & purificação
Masculino
Prevalência
Rickettsia/isolamento & purificação
Tifo por Ácaros/epidemiologia
Tifo por Ácaros/imunologia
Estudos Soroepidemiológicos
Estados Unidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2239-6


  4 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27966221
[Au] Autor:Duan D; Cheng T
[Ad] Endereço:College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, PR China.
[Ti] Título:Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high-throughput sequencing.
[So] Source:J Basic Microbiol;57(4):302-308, 2017 Apr.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this study, we analyzed the in vivo microbial community structure and diversity differences of Haemaphysalis flava (H. flava) in four developmental stages (egg, larva, nymph, and adult) to determine which bacterial genera could be propagated through transovarial transmission and transmitted by transstadial transmission during different developmental stages of H. flava. Paired-end sequencing of the V3 region of the 16S ribosomal DNA (16S rDNA) of H. flava in four developmental stage samples was conducted using the Illumina MiSeq sequencing platform. The following operational taxonomic units (OTUs) of H. flava were obtained during the four developmental stages: 89 of egg, 111 of larva, 104 of nymph, and 106 of female adult tick. Sixty-four of these OTUs had high similarity in the four developmental stages of H. flava. Eight bacterial genera had the highest abundances in all developmental stages, namely, Rickettsia, Coxiella, Pseudomonas, Ehrlichia, Escherichia, Acinetobacter, Citrobacter, and Cupriavidus. The nymph had the highest abundance of Coxiella, and the female adult tick had the highest abundance of Rickettsia. Staphylococcus and Wolbachia were detected in all developmental stages except the egg.
[Mh] Termos MeSH primário: Bactérias/classificação
Bactérias/genética
Ixodidae/crescimento & desenvolvimento
Ixodidae/microbiologia
Consórcios Microbianos/genética
[Mh] Termos MeSH secundário: Acinetobacter/genética
Acinetobacter/isolamento & purificação
Adulto
Animais
Bactérias/isolamento & purificação
Coxiella/genética
Coxiella/isolamento & purificação
DNA Bacteriano/genética
Feminino
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Larva/microbiologia
Ninfa/microbiologia
Óvulo/microbiologia
Filogenia
Pseudomonas/genética
Pseudomonas/isolamento & purificação
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201600557


  5 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27792764
[Au] Autor:Seo MG; Lee SH; Ouh IO; Lee GH; Goo YK; Kim S; Kwon OD; Kwak D
[Ad] Endereço:College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea.
[Ti] Título:Molecular Detection and Genotyping of Coxiella-Like Endosymbionts in Ticks that Infest Horses in South Korea.
[So] Source:PLoS One;11(10):e0165784, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Members of the genus Coxiella can be transmitted from ticks to humans during contact with animals; Coxiella may thus spread from the infected horses or ticks to humans. In this study, the presence of Coxiella burnetii and Coxiella-like endosymbionts (CLE) in ticks found on infested horses was determined using PCR and genotyping. A total of 213 ticks were randomly collected from 51 horses (4-5 ticks per horse) raised on Jeju Island, Korea, between 2009 and 2013. All ticks were morphologically identified as adult Haemaphysalis longicornis, a predominant tick species widespread in Korea. Based on the results of nested PCR and 16S rRNA sequencing, CLE were detected in 121 (52.4%, 95% CI: 45.9-58.8) ticks. CLE 16S rRNA sequences from 9 randomly selected ticks were 100% identical. Phylogenetic analysis showed that these 9 sequences were highly similar (97.9-100%) to the sequences of clade B species, like the CLE previously described to be found in Haemaphysalis spp. This study showed that CLE are prevalent in ticks that infest horses reared on Jeju Island, and this is, to the best of our knowledge, the first study to describe CLE occurrence in ticks infesting animals reared in Korea. Because of the high prevalence of CLE in ticks found on horses, CLE transmission from ticks to other animals and humans remains a possibility. This warrants a detailed study of other hosts and regions. Considering the zoonotic potential of Coxiella, further strategic surveillance of Coxiella transmission is necessary.
[Mh] Termos MeSH primário: Coxiella/genética
Coxiella/fisiologia
Técnicas de Genotipagem
Cavalos/microbiologia
Simbiose
Carrapatos/fisiologia
[Mh] Termos MeSH secundário: Animais
Coxiella/classificação
Filogenia
República da Coreia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0165784


  6 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27763821
[Au] Autor:Khoo JJ; Lim FS; Chen F; Phoon WH; Khor CS; Pike BL; Chang LY; AbuBakar S
[Ad] Endereço:1 Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya , Kuala Lumpur, Malaysia .
[Ti] Título:Coxiella Detection in Ticks from Wildlife and Livestock in Malaysia.
[So] Source:Vector Borne Zoonotic Dis;16(12):744-751, 2016 Dec.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent studies have shown that ticks harbor Coxiella-like bacteria, which are potentially tick-specific endosymbionts. We recently described the detection of Coxiella-like bacteria and possibly Coxiella burnetii in ticks found from rural areas in Malaysia. In the present study, we collected ticks, including Haemaphysalis bispinosa, Haemaphysalis hystricis, Dermacentor compactus, Dermacentor steini, and Amblyomma sp. from wildlife and domesticated goats from four different locations in Malaysia. Coxiella 16s rRNA genomic sequences were detected by PCR in 89% of ticks tested. Similarity analysis and phylogenetic analyses of the 16s rRNA and rpoB partial sequences were performed for 10 representative samples selected based on the tick species, sex, and location. The findings here suggested the presence of C. burnetii in two samples, each from D. steini and H. hystricis. The sequences of both samples clustered with published C. burnetii sequences. The remaining eight tick samples were shown to harbor 16s rRNA sequences of Coxiella-like bacteria, which clustered phylogenetically according to the respective tick host species. The findings presented here added to the growing evidence of the association between Coxiella-like bacteria and ticks across species and geographical boundaries. The importance of C. burnetii found in ticks in Malaysia warrants further investigation.
[Mh] Termos MeSH primário: Animais Selvagens/parasitologia
Coxiella/isolamento & purificação
Gado/parasitologia
Infestações por Carrapato/veterinária
Carrapatos/microbiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
DNA Bacteriano/genética
Feminino
Doenças das Cabras/epidemiologia
Doenças das Cabras/parasitologia
Cabras
Malásia/epidemiologia
Masculino
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Infestações por Carrapato/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


  7 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27595990
[Au] Autor:Machado-Ferreira E; Vizzoni VF; Balsemão-Pires E; Moerbeck L; Gazeta GS; Piesman J; Voloch CM; Soares CA
[Ad] Endereço:Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, 21944-970, Rio de Janeiro, RJ, Brazil.
[Ti] Título:Coxiella symbionts are widespread into hard ticks.
[So] Source:Parasitol Res;115(12):4691-4699, 2016 Dec.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Ticks are blood-feeding arthropods and can harbor several bacteria, including the worldwide zoonotic disease Q-fever agent Coxiella burnetii. Recent studies have reported a distinct group of Coxiella mostly associated with Ixodidae ticks, including the primary endosymbionts of Amblyomma americanum. In the present work, a screening for Coxiella infection was performed by 16S ribosomal DNA (rDNA) gene analyses in 293 tick samples of 15 different species sampled worldwide, including Brazil, Colombia, Kenya, and China. Different Coxiella phylotypes were identified, and these putative symbiotic bacteria were detected in ten different Amblyomma tick species. Approximately 61 % of Rhipicephalus sanguineus and ∼37 % of Rhipicephalus microplus DNA samples were positive for Coxiella. Sequence analysis and phylogenetic reconstruction grouped all the detected Coxiella with Coxiella-like symbionts from different Ixodidae ticks. This well-defined clade clearly excludes known phylotypes of C. burnetii pathogens and other Coxiella spp. detected in different environmental samples and other invertebrate hosts.
[Mh] Termos MeSH primário: Coxiella/isolamento & purificação
Ixodidae/microbiologia
Simbiose
[Mh] Termos MeSH secundário: Animais
Brasil
China
Coxiella/classificação
DNA Ribossômico/genética
Quênia
Tipagem Molecular
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE


  8 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27244561
[Au] Autor:Lee SH; Kim KT; Kwon OD; Ock Y; Kim T; Choi D; Kwak D
[Ad] Endereço:College of Veterinary Medicine, Kyungpook National University, Daegu, South Korea.
[Ti] Título:Novel Detection of Coxiella spp., Theileria luwenshuni, and T. ovis Endosymbionts in Deer Keds (Lipoptena fortisetosa).
[So] Source:PLoS One;11(5):e0156727, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe for the first time the detection of Coxiella-like bacteria (CLB), Theileria luwenshuni, and T. ovis endosymbionts in blood-sucking deer keds. Eight deer keds attached to a Korean water deer were identified as Lipoptena fortisetosa (Diptera: Hippoboscidae) by morphological and genetic analyses. Among the endosymbionts assessed, CLB, Theileria luwenshuni, and T. ovis were identified in L. fortisetosa by PCR and nucleotide sequencing. Based on phylogeny, CLB 16S rRNA sequences were classified into clade B, sharing 99.4% identity with CLB from Haemaphysalis longicornis in South Korea. Although the virulence of CLB to vertebrates is still controversial, several studies have reported clinical symptoms in birds due to CLB infections. The 18S rRNA sequences of T. luwenshuni and T. ovis in this study were 98.8-100% identical to those in GenBank, and all of the obtained sequences of T. ovis and T. luwenshuni in this study were 100% identical to each other, respectively. Although further studies are required to positively confirm L. fortisetosa as a biological vector of these pathogens, strong genetic relationships among sequences from this and previous studies suggest potential transmission among mammalian hosts by ticks and keds.
[Mh] Termos MeSH primário: Coxiella/isolamento & purificação
Dípteros/microbiologia
Dípteros/parasitologia
Infecções por Bactérias Gram-Negativas/transmissão
Infecções por Bactérias Gram-Negativas/veterinária
Theileria/isolamento & purificação
Theileriose/transmissão
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Coxiella/classificação
DNA Bacteriano/genética
DNA de Protozoário/genética
Cervos
Infecções por Bactérias Gram-Negativas/microbiologia
RNA Ribossômico 16S/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
Simbiose
Theileria/classificação
Theileria/genética
Theileriose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Protozoan); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160601
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0156727


  9 / 841 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27132518
[Au] Autor:Khoo JJ; Chen F; Kho KL; Ahmad Shanizza AI; Lim FS; Tan KK; Chang LY; AbuBakar S
[Ad] Endereço:Tropical Infectious Diseases Research and Education Centre (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. Electronic ad
[Ti] Título:Bacterial community in Haemaphysalis ticks of domesticated animals from the Orang Asli communities in Malaysia.
[So] Source:Ticks Tick Borne Dis;7(5):929-937, 2016 Jul.
[Is] ISSN:1877-9603
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ticks are vectors in the transmission of many important infectious diseases in human and animals. Ticks can be readily found in the semi-forested areas such as the settlements of the indigenous people in Malaysia, the Orang Asli. There is still minimal information available on the bacterial agents associated with ticks found in Malaysia. We performed a survey of the bacterial communities associated with ticks collected from domestic animals found in two Orang Asli villages in Malaysia. We collected 62 ticks, microscopically and molecularly identified as related to Haemaphysalis wellingtoni, Haemaphysalis hystricis and Haemaphysalis bispinosa. Bacterial 16s rRNA hypervariable region (V6) amplicon libraries prepared from the tick samples were sequenced on the Ion Torrent PGM platform. We detected a total of 392 possible bacterial genera after pooling and sequencing 20 samples, indicating a diverse bacterial community profile. Dominant taxa include the potential tick endosymbiont, Coxiella. Other dominant taxa include the tick-associated pathogen, Rickettsia, and environmental bacteria such as Bacillus, Mycobacterium, Sphingomonas and Pseudomonas. Other known tick-associated bacteria were also detected, including Anaplasma, Ehrlichia, Rickettsiella and Wolbachia, albeit at very low abundance. Specific PCR was performed on selected samples to identify Rickettsia and Coxiella. Sequence of Rickettsia felis, which causes spotted fever in human and cats, was identified in one sample. Coxiella endosymbionts were detected in three samples. This study provides the baseline knowledge of the microbiome of ticks in Malaysia, focusing on tick-associated bacteria affecting the Orang Asli communities. The role of the herein found Coxiella and Rickettsia in tick physiology or disease transmission merits further investigation.
[Mh] Termos MeSH primário: Animais Domésticos
Bactérias/isolamento & purificação
Ixodidae/microbiologia
Microbiota
Infestações por Carrapato/veterinária
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma/isolamento & purificação
Animais
Bacillus/genética
Bacillus/isolamento & purificação
Gatos/parasitologia
Galinhas/parasitologia
Coxiella/genética
Coxiella/isolamento & purificação
DNA Bacteriano/genética
Cães/parasitologia
Ehrlichia/genética
Ehrlichia/isolamento & purificação
Malásia/epidemiologia
Mycobacterium/genética
Mycobacterium/isolamento & purificação
Filogenia
Reação em Cadeia da Polimerase
Pseudomonas/genética
Pseudomonas/isolamento & purificação
RNA Ribossômico 16S/genética
Rickettsia/genética
Rickettsia/isolamento & purificação
Sphingomonas/genética
Sphingomonas/isolamento & purificação
Infestações por Carrapato/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE


  10 / 841 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26999394
[Au] Autor:Di Russo Case E; Samuel JE
[Ti] Título:Contrasting Lifestyles Within the Host Cell.
[So] Source:Microbiol Spectr;4(1), 2016 Feb.
[Is] ISSN:2165-0497
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intracellular bacterial pathogens have evolved to exploit the protected niche provided within the boundaries of a eukaryotic host cell. Upon entering a host cell, some bacteria can evade the adaptive immune response of its host and replicate in a relatively nutrient-rich environment devoid of competition from other host flora. Growth within a host cell is not without their hazards, however. Many pathogens enter their hosts through receptor-mediated endocytosis or phagocytosis, two intracellular trafficking pathways that terminate in a highly degradative organelle, the phagolysosome. This usually deadly compartment is maintained at a low pH and contains degradative enzymes and reactive oxygen species, resulting in an environment to which few bacterial species are adapted. Some intracellular pathogens, such as Shigella, Listeria, Francisella, and Rickettsia, escape the phagosome to replicate within the cytosol of the host cell. Bacteria that remain within a vacuole either alter the trafficking of their initial phagosomal compartment or adapt to survive within the harsh environment it will soon become. In this chapter, we focus on the mechanisms by which different vacuolar pathogens either evade lysosomal fusion, as in the case of Mycobacterium and Chlamydia, or allow interaction with lysosomes to varying degrees, such as Brucella and Coxiella, and their specific adaptations to inhabit a replicative niche.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos Bacterianos
Células Eucarióticas/microbiologia
[Mh] Termos MeSH secundário: Adaptação Psicológica
Animais
Bactérias
Infecções Bacterianas/microbiologia
Brucella/fisiologia
Chlamydia/fisiologia
Coxiella/fisiologia
Seres Humanos
Lisossomos/microbiologia
Mycobacterium/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160322
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.VMBF-0014-2015



página 1 de 85 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde