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Pesquisa : B03.440.400.425.500.505 [Categoria DeCS]
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[PMID]:27646453
[Au] Autor:Zhang Y; Chen JX; Wen LL; Tang Y; Zhao HP
[Ad] Endereço:Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou, China.
[Ti] Título:Effects of salinity on simultaneous reduction of perchlorate and nitrate in a methane-based membrane biofilm reactor.
[So] Source:Environ Sci Pollut Res Int;23(23):24248-24255, 2016 Dec.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study builds upon prior work showing that methane (CH ) could be utilized as the sole electron donor and carbon source in a membrane biofilm reactor (MBfR) for complete perchlorate (ClO ) and nitrate (NO ) removal. Here, we further investigated the effects of salinity on the simultaneous removal of the two contaminants in the reactor. By testing ClO and NO at different salinities, we found that the reactor performance was very sensitive to salinity. While 0.2 % salinity did not significantly affect the hydrogen-based MBfR for ClO and NO removals, 1 % salinity completely inhibited ClO reduction and significantly lowered NO reduction in the CH -based MBfR. In salinity-free conditions, NO and ClO removal fluxes were 0.171 g N/m -day and 0.091 g/m -day, respectively, but NO removal fluxes dropped to 0.0085 g N/m -day and ClO reduction was completely inhibited when the medium changed to 1 % salinity. Scanning electron microscopy (SEM) showed that the salinity dramatically changed the microbial morphology, which led to the development of wire-like cell structures. Quantitative real-time PCR (qPCR) indicated that the total number of microorganisms and abundances of functional genes significantly declined in the presence of NaCl. The relative abundances of Methylomonas (methanogens) decreased from 31.3 to 5.9 % and Denitratisoma (denitrifiers) decreased from 10.6 to 4.4 % when 1 % salinity was introduced.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Reatores Biológicos/microbiologia
Metano/metabolismo
Nitratos/análise
Percloratos/análise
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Membranas Artificiais
Mathanococcus/crescimento & desenvolvimento
Mathanococcus/metabolismo
Methylocystaceae/crescimento & desenvolvimento
Methylocystaceae/metabolismo
Methylomonas/crescimento & desenvolvimento
Methylomonas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Salinidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Nitrates); 0 (Perchlorates); 0 (Water Pollutants, Chemical); OP0UW79H66 (Methane); VLA4NZX2P4 (perchlorate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


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[PMID]:27326439
[Ti] Título:Methylotroph [meth'il-o-trof"].
[So] Source:Emerg Infect Dis;22(3):409, 2016 Mar.
[Is] ISSN:1080-6059
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Bactérias/classificação
Bactérias/metabolismo
[Mh] Termos MeSH secundário: Methylomonas/classificação
Methylomonas/metabolismo
Terminologia como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE


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[PMID]:25590822
[Au] Autor:Peng YH; Chen YJ; Chang YJ; Shih YH
[Ad] Endereço:Department of Agricultural Chemistry, National Taiwan University, Taipei City 10617, Taiwan. Electronic address: yuhuei97@gmail.com.
[Ti] Título:Biodegradation of bisphenol A with diverse microorganisms from river sediment.
[So] Source:J Hazard Mater;286:285-90, 2015 Apr 09.
[Is] ISSN:1873-3336
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The wide distribution of bisphenol A (BPA) in the environment is problematic because of its endocrine-disrupting characteristics and toxicity. Developing cost-effective remediation methods for wide implementation is crucial. Therefore, this study investigated the BPA biodegradation ability of various microorganisms from river sediment. An acclimated microcosm completely degraded 10 mg L(-1) BPA within 28 h and transformed the contaminant into several metabolic intermediates. During the degradation process, the microbial compositions fluctuated and the final, predominant microorganisms were Pseudomonas knackmussii and Methylomonas clara. From the original river sediment, we isolated four distinct strains, which deplete the BPA over 7-9 days. They were all genetically similar to P. knackmussii. The degradation ability of mixed strains was higher than that of single strain but was far less than that of the microbial consortium. The novel BPA degradation ability of P. knackmussii and its role in the decomposing microcosm were first demonstrated. Our results revealed that microbial diversity plays a crucial role in pollutant decomposition.
[Mh] Termos MeSH primário: Compostos Benzidrílicos/isolamento & purificação
Sedimentos Geológicos/microbiologia
Fenóis/isolamento & purificação
Rios/química
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cinética
Methylomonas/crescimento & desenvolvimento
Pseudomonas/crescimento & desenvolvimento
Rios/microbiologia
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzhydryl Compounds); 0 (Phenols); 0 (Water Pollutants, Chemical); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150320
[Lr] Data última revisão:
150320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150116
[St] Status:MEDLINE


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[PMID]:25581131
[Au] Autor:He R; Wooller MJ; Pohlman JW; Tiedje JM; Leigh MB
[Ad] Endereço:Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
[Ti] Título:Methane-derived carbon flow through microbial communities in arctic lake sediments.
[So] Source:Environ Microbiol;17(9):3233-50, 2015 Sep.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aerobic methane (CH4 ) oxidation mitigates CH4 release and is a significant pathway for carbon and energy flow into aquatic food webs. Arctic lakes are responsible for an increasing proportion of global CH4 emissions, but CH4 assimilation into the aquatic food web in arctic lakes is poorly understood. Using stable isotope probing (SIP) based on phospholipid fatty acids (PLFA-SIP) and DNA (DNA-SIP), we tracked carbon flow quantitatively from CH4 into sediment microorganisms from an arctic lake with an active CH4 seepage. When 0.025 mmol CH4 g(-1) wet sediment was oxidized, approximately 15.8-32.8% of the CH4 -derived carbon had been incorporated into microorganisms. This CH4 -derived carbon equated to up to 5.7% of total primary production estimates for Alaskan arctic lakes. Type I methanotrophs, including Methylomonas, Methylobacter and unclassified Methylococcaceae, were most active at CH4 oxidation in this arctic lake. With increasing distance from the active CH4 seepage, a greater diversity of bacteria incorporated CH4 -derived carbon. Actinomycetes were the most quantitatively important microorganisms involved in secondary feeding on CH4 -derived carbon. These results showed that CH4 flows through methanotrophs into the broader microbial community and that type I methanotrophs, methylotrophs and actinomycetes are important organisms involved in using CH4 -derived carbon in arctic freshwater ecosystems.
[Mh] Termos MeSH primário: Ciclo do Carbono
Sedimentos Geológicos/microbiologia
Lagos/microbiologia
Metano/metabolismo
Consórcios Microbianos
Microbiologia da Água
[Mh] Termos MeSH secundário: Actinobacteria/genética
Actinobacteria/metabolismo
Regiões Árticas
Carbono/metabolismo
Isótopos de Carbono/análise
Ecossistema
Ácidos Graxos/metabolismo
Sedimentos Geológicos/química
Methylobacteriaceae/genética
Methylobacteriaceae/metabolismo
Methylomonas/genética
Methylomonas/metabolismo
Oxirredução
Fosfolipídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Fatty Acids); 0 (Phospholipids); 7440-44-0 (Carbon); OP0UW79H66 (Methane)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150909
[Lr] Data última revisão:
150909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150113
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.12773


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[PMID]:25580993
[Au] Autor:Kits KD; Klotz MG; Stein LY
[Ad] Endereço:Department of Biological Sciences, University of Alberta, CW405, Biological Sciences Building, Edmonton, AB, T6G 2E9, Canada.
[Ti] Título:Methane oxidation coupled to nitrate reduction under hypoxia by the Gammaproteobacterium Methylomonas denitrificans, sp. nov. type strain FJG1.
[So] Source:Environ Microbiol;17(9):3219-32, 2015 Sep.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Obligate methanotrophs belonging to the Phyla Proteobacteria and Verrucomicrobia require oxygen for respiration and methane oxidation; nevertheless, aerobic methanotrophs are abundant and active in low oxygen environments. While genomes of some aerobic methanotrophs encode putative nitrogen oxide reductases, it is not understood whether these metabolic modules are used for NOx detoxification, denitrification or other purposes. Here we demonstrate using microsensor measurements that a gammaproteobacterial methanotroph Methylomonas denitrificans sp. nov. strain FJG1(T) couples methane oxidation to nitrate reduction under oxygen limitation, releasing nitrous oxide as a terminal product. Illumina RNA-Seq data revealed differential expression of genes encoding a denitrification pathway previously unknown to methanotrophs as well as the pxmABC operon in M. denitrificans sp. nov. strain FJG1(T) in response to hypoxia. Physiological and transcriptome data indicate that genetic inventory encoding the denitrification pathway is upregulated only upon availability of nitrate under oxygen limitation. In addition, quantitation of ATP levels demonstrates that the denitrification pathway employs inventory such as nitrate reductase NarGH serving M. denitrificans sp. nov. strain FJG1(T) to conserve energy during oxygen limitation. This study unravelled an unexpected metabolic flexibility of aerobic methanotrophs, thereby assigning these bacteria a new role at the metabolic intersection of the carbon and nitrogen cycles.
[Mh] Termos MeSH primário: Metano/metabolismo
Methylomonas/metabolismo
Nitratos/metabolismo
Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Anaerobiose
Desnitrificação/genética
Methylomonas/classificação
Methylomonas/genética
Nitrato Redutase/genética
Óxido Nitroso/metabolismo
Oxirredução
Oxirredutases/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Nitrates); EC 1.- (Oxidoreductases); EC 1.7.2.4 (nitrous oxide reductase); EC 1.7.99.4 (Nitrate Reductase); K50XQU1029 (Nitrous Oxide); OP0UW79H66 (Methane); S88TT14065 (Oxygen)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150909
[Lr] Data última revisão:
150909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150113
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.12772


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[PMID]:25186436
[Au] Autor:Henneberger R; Chiri E; Bodelier PE; Frenzel P; Lüke C; Schroth MH
[Ad] Endereço:Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, 8092, Zurich, Switzerland.
[Ti] Título:Field-scale tracking of active methane-oxidizing communities in a landfill cover soil reveals spatial and seasonal variability.
[So] Source:Environ Microbiol;17(5):1721-37, 2015 May.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aerobic methane-oxidizing bacteria (MOB) in soils mitigate methane (CH4 ) emissions. We assessed spatial and seasonal differences in active MOB communities in a landfill cover soil characterized by highly variable environmental conditions. Field-based measurements of CH4 oxidation activity and stable-isotope probing of polar lipid-derived fatty acids (PLFA-SIP) were complemented by microarray analysis of pmoA genes and transcripts, linking diversity and function at the field scale. In situ CH4 oxidation rates varied between sites and were generally one order of magnitude lower in winter compared with summer. Results from PLFA-SIP and pmoA transcripts were largely congruent, revealing distinct spatial and seasonal clustering. Overall, active MOB communities were highly diverse. Type Ia MOB, specifically Methylomonas and Methylobacter, were key drivers for CH4 oxidation, particularly at a high-activity site. Type II MOB were mainly active at a site showing substantial fluctuations in CH4 loading and soil moisture content. Notably, Upland Soil Cluster-gamma-related pmoA transcripts were also detected, indicating concurrent oxidation of atmospheric CH4 . Spatial separation was less distinct in winter, with Methylobacter and uncultured MOB mediating CH4 oxidation. We propose that high diversity of active MOB communities in this soil is promoted by high variability in environmental conditions, facilitating substantial removal of CH4 generated in the waste body.
[Mh] Termos MeSH primário: Metano/metabolismo
Methylomonas/metabolismo
Microbiologia do Solo
Instalações de Eliminação de Resíduos
[Mh] Termos MeSH secundário: Ácidos Graxos/metabolismo
Methylomonas/classificação
Methylomonas/genética
Oxirredução
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids); OP0UW79H66 (Methane)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150427
[Lr] Data última revisão:
150427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140905
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.12617


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[PMID]:24785289
[Au] Autor:Ho A; de Roy K; Thas O; De Neve J; Hoefman S; Vandamme P; Heylen K; Boon N
[Ad] Endereço:Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
[Ti] Título:The more, the merrier: heterotroph richness stimulates methanotrophic activity.
[So] Source:ISME J;8(9):1945-8, 2014 Sep.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although microorganisms coexist in the same environment, it is still unclear how their interaction regulates ecosystem functioning. Using a methanotroph as a model microorganism, we determined how methane oxidation responds to heterotroph diversity. Artificial communities comprising of a methanotroph and increasing heterotroph richness, while holding equal starting cell numbers were assembled. We considered methane oxidation rate as a functional response variable. Our results showed a significant increase of methane oxidation with increasing heterotroph richness, suggesting a complex interaction in the cocultures leading to a stimulation of methanotrophic activity. Therefore, not only is the methanotroph diversity directly correlated to methanotrophic activity for some methanotroph groups as shown before, but also the richness of heterotroph interacting partners is relevant to enhance methane oxidation too. In this unprecedented study, we provide direct evidence showing how heterotroph richness exerts a response in methanotroph-heterotroph interaction, resulting in increased methanotrophic activity. Our study has broad implications in how methanotroph and heterotroph interact to regulate methane oxidation, and is particularly relevant in methane-driven ecosystems.
[Mh] Termos MeSH primário: Biodiversidade
Processos Heterotróficos
Metano/metabolismo
[Mh] Termos MeSH secundário: Bactérias/metabolismo
Methylomonas/metabolismo
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
OP0UW79H66 (Methane)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:150902
[Lr] Data última revisão:
150902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140503
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2014.74


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[PMID]:24708438
[Au] Autor:Hoefman S; van der Ha D; Boon N; Vandamme P; De Vos P; Heylen K
[Ti] Título:Niche differentiation in nitrogen metabolism among methanotrophs within an operational taxonomic unit.
[So] Source:BMC Microbiol;14:83, 2014 Apr 04.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The currently accepted thesis on nitrogenous fertilizer additions on methane oxidation activity assumes niche partitioning among methanotrophic species, with activity responses to changes in nitrogen content being dependent on the in situ methanotrophic community structure Unfortunately, widely applied tools for microbial community assessment only have a limited phylogenetic resolution mostly restricted to genus level diversity, and not to species level as often mistakenly assumed. As a consequence, intragenus or intraspecies metabolic versatility in nitrogen metabolism was never evaluated nor considered among methanotrophic bacteria as a source of differential responses of methane oxidation to nitrogen amendments. RESULTS: We demonstrated that fourteen genotypically different Methylomonas strains, thus distinct below the level at which most techniques assign operational taxonomic units (OTU), show a versatile physiology in their nitrogen metabolism. Differential responses, even among strains with identical 16S rRNA or pmoA gene sequences, were observed for production of nitrite and nitrous oxide from nitrate or ammonium, nitrogen fixation and tolerance to high levels of ammonium, nitrate, and hydroxylamine. Overall, reduction of nitrate to nitrite, nitrogen fixation, higher tolerance to ammonium than nitrate and tolerance and assimilation of nitrite were general features. CONCLUSIONS: Differential responses among closely related methanotrophic strains to overcome inhibition and toxicity from high nitrogen loads and assimilation of various nitrogen sources yield competitive fitness advantages to individual methane-oxidizing bacteria. Our observations proved that community structure at the deepest phylogenetic resolution potentially influences in situ functioning.
[Mh] Termos MeSH primário: Metano/metabolismo
Methylomonas/classificação
Methylomonas/metabolismo
Nitrogênio/metabolismo
[Mh] Termos MeSH secundário: Compostos de Amônio/metabolismo
DNA Bacteriano/classificação
DNA Bacteriano/genética
Tolerância a Medicamentos
Dados de Sequência Molecular
Nitratos/metabolismo
Nitritos/metabolismo
Fixação de Nitrogênio
Óxido Nitroso/metabolismo
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (DNA, Bacterial); 0 (Nitrates); 0 (Nitrites); 0 (RNA, Ribosomal, 16S); K50XQU1029 (Nitrous Oxide); N762921K75 (Nitrogen); OP0UW79H66 (Methane)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140409
[St] Status:MEDLINE
[do] DOI:10.1186/1471-2180-14-83


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[PMID]:24408530
[Au] Autor:Hoefman S; Heylen K; De Vos P
[Ad] Endereço:Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium.
[Ti] Título:Methylomonas lenta sp. nov., a methanotroph isolated from manure and a denitrification tank.
[So] Source:Int J Syst Evol Microbiol;64(Pt 4):1210-7, 2014 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two methanotrophic bacteria, strains R-45377(T) and R-45370, were respectively isolated from a slurry pit of a cow stable and from a denitrification tank of a wastewater treatment plant in Belgium. The strains showed 99.9 % 16S rRNA gene sequence similarity. Cells were Gram-negative, motile rods containing type I methanotroph intracytoplasmic membranes. Colonies and liquid cultures appeared white to pale pink. The pmoA gene encoding particulate methane monooxygenase (pMMO) and the nifH gene encoding nitrogenase were present. Soluble methane monooxygenase (sMMO) activity, the presence of the mmoX gene encoding sMMO and the presence of the pxmA gene encoding a sequence-divergent pMMO were not detected. Methane and methanol were utilized as sole carbon sources. The strains grew optimally at 20 °C (range 15-28 °C) and at pH 6.8-7.3 (range pH 6.3-7.8). The strains grew in media supplemented with up to 1.2 % NaCl. The major cellular fatty acids were C16 : 1ω8c, C16 : 1ω5c, C16 : 1ω7c, C14 : 0, C15 : 0 and C16 : 0 and the DNA G+C content was 47 mol%. 16S rRNA gene- and pmoA-based phylogenetic analyses showed that the isolates cluster among members of the genus Methylomonas within the class Gammaproteobacteria, with pairwise 16S rRNA gene sequence similarities of 97.5 and 97.2 % between R-45377(T) and the closest related type strains, Methylomonas scandinavica SR5(T) and Methylomonas paludis MG30(T), respectively. Based on phenotypic characterization of strains R-45377(T) and R-45370, their low 16S rRNA gene sequence similarities and the formation of a separate phylogenetic lineage compared with existing species of the genus Methylomonas, we propose to classify these strains in a novel species, Methylomonas lenta sp. nov., with R-45377(T) ( = LMG 26260(T) = JCM 19378(T)) as the type strain.
[Mh] Termos MeSH primário: Desnitrificação
Esterco/microbiologia
Methylomonas/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Bélgica
Bovinos
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
Metano/metabolismo
Methylomonas/genética
Methylomonas/isolamento & purificação
Dados de Sequência Molecular
Fixação de Nitrogênio
Oxirredutases/genética
Oxigenases/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Eliminação de Resíduos Líquidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Manure); 0 (RNA, Ribosomal, 16S); EC 1.- (Oxidoreductases); EC 1.13.- (Oxygenases); EC 1.14.13.25 (methane monooxygenase); EC 1.18.6.1 (nitrogenase reductase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:140407
[Lr] Data última revisão:
140407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140111
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.057794-0


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[PMID]:24271576
[Au] Autor:Hoefman S; van der Ha D; Boon N; Vandamme P; De Vos P; Heylen K
[Ad] Endereço:Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
[Ti] Título:Customized media based on miniaturized screening improve growth rate and cell yield of methane-oxidizing bacteria of the genus Methylomonas.
[So] Source:Antonie Van Leeuwenhoek;105(2):353-66, 2014 Feb.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The growth of twelve methanotrophic strains within the genus Methylomonas, including the type strains of Methylomonas methanica and Methylomonas koyamae, was evaluated with 40 different variations of standard diluted nitrate mineral salts medium in 96-well microtiter plates. Unique profiles of growth preference were observed for each strain, showing a strong strain dependency for optimal growth conditions, especially with regards to the preferred concentration and nature of the nitrogen source. Based on the miniaturized screening results, a customized medium was designed for each strain, allowing the improvement of the growth of several strains in a batch setup, either by a reduction of the lag phase or by faster biomass accumulation. As such, the maintenance of fastidious strains could be facilitated while the growth of fast-growing Methylomonas strains could be further improved. Methylomonas sp. R-45378 displayed a 50 % increase in cell dry weight when grown in its customized medium and showed the lowest observed nitrogen and oxygen requirement of all tested strains. We demonstrate that the presented miniaturized approach for medium optimization is a simple tool allowing the quick generation of strain-specific growth preference data that can be applied downstream of an isolation campaign. This approach can also be applied as a first step in the search for strains with biotechnological potential, to facilitate cultivation of fastidious strains or to steer future isolation campaigns.
[Mh] Termos MeSH primário: Meios de Cultura/química
Methylococcaceae/crescimento & desenvolvimento
Methylococcaceae/isolamento & purificação
Methylomonas/crescimento & desenvolvimento
Methylomonas/isolamento & purificação
[Mh] Termos MeSH secundário: Técnicas Bacteriológicas/métodos
Nitrogênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); N762921K75 (Nitrogen)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140116
[Lr] Data última revisão:
140116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131126
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-013-0083-2



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