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Pesquisa : B03.440.400.425.537 [Categoria DeCS]
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  1 / 27 MEDLINE  
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[PMID]:28968382
[Au] Autor:Salter SJ; Turner C; Watthanaworawit W; de Goffau MC; Wagner J; Parkhill J; Bentley SD; Goldblatt D; Nosten F; Turner P
[Ad] Endereço:Pathogen Genomics, Wellcome Trust Sanger Institute, Hinxton, United Kingdom.
[Ti] Título:A longitudinal study of the infant nasopharyngeal microbiota: The effects of age, illness and antibiotic use in a cohort of South East Asian children.
[So] Source:PLoS Negl Trop Dis;11(10):e0005975, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A longitudinal study was undertaken in infants living in the Maela refugee camp on the Thailand-Myanmar border between 2007 and 2010. Nasopharyngeal swabs were collected monthly, from birth to 24 months of age, with additional swabs taken if the infant was diagnosed with pneumonia according to WHO clinical criteria. At the time of collection, swabs were cultured for Streptococcus pneumoniae and multiple serotype carriage was assessed. The bacterial 16S rRNA gene profiles of 544 swabs from 21 infants were analysed to see how the microbiota changes with age, respiratory infection, antibiotic consumption and pneumococcal acquisition. The nasopharyngeal microbiota is a somewhat homogenous community compared to that of other body sites. In this cohort it is dominated by five taxa: Moraxella, Streptococcus, Haemophilus, Corynebacterium and an uncharacterized Flavobacteriaceae taxon of 93% nucleotide similarity to Ornithobacterium. Infant age correlates with certain changes in the microbiota across the cohort: Staphylococcus and Corynebacterium are associated with the first few months of life while Moraxella and the uncharacterised Flavobacteriaceae increase in proportional abundance with age. Respiratory illness and antibiotic use often coincide with an unpredictable perturbation of the microbiota that differs from infant to infant and in different illness episodes. The previously described interaction between Dolosigranulum and Streptococcus was observed in these data. Monthly sampling demonstrates that the nasopharyngeal microbiota is in flux throughout the first two years of life, and that in this refugee camp population the pool of potential bacterial colonisers may be limited.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Bactérias/isolamento & purificação
Microbiota
Nasofaringe/microbiologia
Infecções Respiratórias/epidemiologia
[Mh] Termos MeSH secundário: Fatores Etários
Antibacterianos/efeitos adversos
Bactérias/classificação
Bactérias/genética
Portador Sadio/epidemiologia
Portador Sadio/microbiologia
Estudos de Coortes
Feminino
Seres Humanos
Lactente
Recém-Nascido
Estudos Longitudinais
Masculino
Moraxellaceae/genética
Moraxellaceae/isolamento & purificação
Pneumonia/epidemiologia
RNA Ribossômico 16S/genética
Refugiados
Infecções Respiratórias/microbiologia
Streptococcus pneumoniae/genética
Streptococcus pneumoniae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005975


  2 / 27 MEDLINE  
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[PMID]:27475037
[Au] Autor:Wang X; Zhao X; Li H; Jia J; Liu Y; Ejenavi O; Ding A; Sun Y; Zhang D
[Ad] Endereço:Lancaster Environment Center, Lancaster University, Lancaster LA1 4YQ, UK.
[Ti] Título:Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation.
[So] Source:Res Microbiol;167(9-10):731-744, 2016 Nov - Dec.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Uncultivable microorganisms account for over 99% of all species on the planet, but their functions are yet not well characterized. Though many cultivable degraders for n-alkanes have been intensively investigated, the roles of functional n-alkane degraders remain hidden in the natural environment. This study introduces the novel magnetic nanoparticle-mediated isolation (MMI) technology in Nigerian soils and successfully separates functional microbes belonging to the families Oxalobacteraceae and Moraxellaceae, which are dominant and responsible for alkane metabolism in situ. The alkR-type n-alkane monooxygenase genes, instead of alkA- or alkP-type, were the key functional genes involved in the n-alkane degradation process. Further physiological investigation via a BIOLOG PM plate revealed some carbon (Tween 20, Tween 40 and Tween 80) and nitrogen (tyramine, l-glutamine and d-aspartic acid) sources promoting microbial respiration and n-alkane degradation. With further addition of promoter carbon or nitrogen sources, the separated functional alkane degraders significantly improved n-alkane biodegradation rates. This suggests that MMI is a promising technology for separating functional microbes from complex microbiota, with deeper insight into their ecological functions and influencing factors. The technique also broadens the application of the BIOLOG PM plate for physiological research on functional yet uncultivable microorganisms.
[Mh] Termos MeSH primário: Alcanos/metabolismo
Técnicas Bacteriológicas/métodos
Moraxellaceae/isolamento & purificação
Oxalobacteraceae/isolamento & purificação
Petróleo/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Carbono/metabolismo
Magnetismo
Metaboloma
Análise em Microsséries
Nanopartículas
Nitrogênio/metabolismo
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkanes); 0 (Petroleum); 0 (Soil Pollutants); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160801
[St] Status:MEDLINE


  3 / 27 MEDLINE  
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[PMID]:26965608
[Au] Autor:Kupferschmidt K
[Ti] Título:The microbial death clock.
[So] Source:Science;351(6278):1143, 2016 Mar 11.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Acinetobacter/crescimento & desenvolvimento
Morte
Moraxellaceae/crescimento & desenvolvimento
Rhizobiaceae/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Camundongos
Fatores de Tempo
[Pt] Tipo de publicação:NEWS
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160311
[Lr] Data última revisão:
160311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE
[do] DOI:10.1126/science.351.6278.1143


  4 / 27 MEDLINE  
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[PMID]:26795079
[Au] Autor:Pokorny B; Kosma P
[Ad] Endereço:Department of Chemistry, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
[Ti] Título:Synthesis of 5-O-oligoglucosyl extended α-(2→4)-Kdo disaccharides corresponding to inner core fragments of Moraxellaceae lipopolysaccharides.
[So] Source:Carbohydr Res;422:5-12, 2016 Mar 03.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The heptose-deficient inner core of the lipopolysaccharide of several pathogenic strains of the Moraxellaceae family (Moraxella, Acinetobacter) and of Bartonella henselae, respectively, comprises an α-D-glucopyranose attached to position 5 of Kdo. In continuation of the synthesis of fragments of Acinetobacter haemolyticus LPS, the branched α-Glcp-(1 → 5)[α-Kdo-(2 → 4)]-α-Kdo trisaccharide motif was elaborated. The glycosylation of a suitably protected, α-(2 → 4)-interlinked Kdo-disaccharide was achieved in high yield and fair anomeric selectivity using a 4,6-O-benzylidene N-phenyltrifluoroacetimidate glucosyl donor. Subsequent regioselective reductive benzylidene opening afforded a trisaccharide acceptor, which was extended with ß-D-glucopyranosyl and isomaltosyl residues. Global deprotection provided tri- to pentasaccharide structures corresponding to the inner core region of A. haemolyticus lipopolysaccharide.
[Mh] Termos MeSH primário: Dissacarídeos/química
Dissacarídeos/síntese química
Glucose/química
Lipopolissacarídeos/química
Moraxellaceae/química
[Mh] Termos MeSH secundário: Técnicas de Química Sintética
Estereoisomerismo
Trissacarídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Disaccharides); 0 (Lipopolysaccharides); 0 (Trisaccharides); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160123
[St] Status:MEDLINE


  5 / 27 MEDLINE  
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[PMID]:26498187
[Au] Autor:Kim MK; Kim TW; Kim TS; Joung Y; Han JH; Kim SB
[Ad] Endereço:1​ Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 34134, Republic of Korea.
[Ti] Título:Fluviicoccus keumensis gen. nov., sp. nov., isolated from freshwater.
[So] Source:Int J Syst Evol Microbiol;66(1):201-5, 2016 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, non-spore-forming and coccus-shaped bacterial strain, designated 4DR5T, was isolated from freshwater and its taxonomic position was investigated using a polyphasic approach. Growth occurred at 10-40 °C (optimum 30 °C), at pH 6-9 (optimum pH 7) and in the presence of 0-0.4 % (w/v) NaCl (optimum 0 %) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain 4DR5T was assigned to the family Moraxellaceae of the class Gammaproteobacteria, and its closest related taxa were species of the genera Perlucidibaca (93.67 % sequence similarity), Agitococcus (93.07 %), Paraperlucidibaca (92.31-92.38 %), Alkanindiges (91.79 %) and Acinetobacter (90.24-91.23 %). The predominant isoprenoid quinone detected in strain 4DR5T was Q-10. The major cellular fatty acids were a summed feature consisting of C16 : 1ω7c and/or C16 : 1ω6c, one consisting of C18 : 1ω7c and/or C18 : 1ω6c, and C16 : 0. The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of the strain was 61.2 mol%. The phylogenetic, chemotaxonomic and biochemical data not only supported the affiliation of strain 4DR5T to the family Moraxellaceae, but also separated it from other established genera within the family. Therefore, the novel isolate evidently represents a novel species of a new genus of Moraxellaceae, for which the name Fluviicoccus keumensis gen. nov., sp. nov. is proposed. The type strain of Fluviicoccus keumensis is 4DR5T ( = KCTC 32475T = JCM 19370T).
[Mh] Termos MeSH primário: Água Doce/microbiologia
Moraxellaceae/classificação
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Dados de Sequência Molecular
Moraxellaceae/genética
Moraxellaceae/isolamento & purificação
Fosfatidiletanolaminas/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Ubiquinona/análogos & derivados
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 39382-08-6 (phosphatidylethanolamine); EJ27X76M46 (coenzyme Q10)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160212
[Lr] Data última revisão:
160212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151027
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000694


  6 / 27 MEDLINE  
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[PMID]:25267870
[Au] Autor:Humphreys GJ; Oates A; Ledder RG; McBain AJ
[Ad] Endereço:Manchester Pharmacy School, Oxford Road, University of Manchester, Manchester M13 9PT, UK gavin.humphreys@manchester.ac.uk.
[Ti] Título:Faucicola mancuniensis gen. nov., sp. nov., isolated from the human oropharynx.
[So] Source:Int J Syst Evol Microbiol;65(Pt 1):11-14, 2015 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An aerobic, Gram-stain-negative, non-motile coccus, designated strain GVCNT2(T), was isolated from the tonsils of a healthy adult female. Cells were oxidase- and catalase-positive, positive for the production of esterase (C4), esterase lipase (C8) and leucine arylamidase, and weakly positive for naphthol-AS-BI-phosphohydrolase and alkaline phosphatase. Cells were also capable of hydrolysing DNA. Growth was observed at 20-37 °C and in the presence of up to 1.5% NaCl. Phylogenetic analysis of near full-length 16S rRNA gene sequences indicated that the strain exhibited closest sequence similarity to Moraxella boevrei ATCC 700022(T) (94.68%) and an uncultured, unspeciated bacterial clone (strain S12-08; 99%). The major fatty acids were C18:1ω9c, C18 : 0, C16:0 and C16:1ω6c/C16:1ω7c. The DNA G+C content of strain GVCNT2(T) was 40.7 mol%. The major respiratory quinone identified was Q-8. Strain GVCNT2(T) exhibited a comparable phenotypic profile to other members of the genus Moraxella but could be distinguished based on its ability to produce acid (weakly) from d-glucose, melibiose, l-arabinose and rhamnose and on its ability to hydrolyse DNA. On the basis of phenotypic and phylogenetic differences from other members of the family Moraxellaceae, strain GVCNT2(T) is considered to represent a novel species of a new genus, for which the name Faucicola mancuniensis gen. nov., sp. nov. is proposed. The type strain of Faucicola mancuniensis is GVCNT2(T) ( =DSM 28411(T) =NCIMB 14946(T)).
[Mh] Termos MeSH primário: Moraxellaceae/classificação
Orofaringe/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Feminino
Seres Humanos
Dados de Sequência Molecular
Moraxellaceae/genética
Moraxellaceae/isolamento & purificação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141001
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.066837-0


  7 / 27 MEDLINE  
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[PMID]:24462428
[Au] Autor:Nakayama A; Yamanaka K; Hayashi H; Ohkusu K
[Ad] Endereço:Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University, School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
[Ti] Título:Moraxella lacunata infection associated with septicemia, endocarditis, and bilateral septic arthritis in a patient undergoing hemodialysis: a case report and review of the literature.
[So] Source:J Infect Chemother;20(1):61-4, 2014 Jan.
[Is] ISSN:1437-7780
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We report the first case of both endocarditis and bilateral septic arthritis in a patient caused by Moraxella lacunata and successful management of the infection with antimicrobial therapy. The route of entry leading to bacteremia may have been the oral cavity given the poor oral hygiene of the patient as evidenced by bleeding gums. We hypothesize that the bacteremia led to septic arthritis and mitral valve infective endocarditis. In this case report, we also review the literature on M. lacunata infections and conclude that this organism should be considered in bilateral septic arthritis in a patient with underlying heart abnormalities and/or with renal failure.
[Mh] Termos MeSH primário: Artrite Infecciosa/microbiologia
Bacteriemia/microbiologia
Endocardite Bacteriana/microbiologia
Infecções por Moraxellaceae/microbiologia
Sepse/microbiologia
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/uso terapêutico
Artrite Infecciosa/tratamento farmacológico
Bacteriemia/tratamento farmacológico
Endocardite Bacteriana/tratamento farmacológico
Feminino
Seres Humanos
Moraxellaceae/efeitos dos fármacos
Infecções por Moraxellaceae/tratamento farmacológico
Diálise Renal
Sepse/tratamento farmacológico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140217
[Lr] Data última revisão:
140217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140128
[St] Status:MEDLINE


  8 / 27 MEDLINE  
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[PMID]:24450178
[Au] Autor:Romanovskaia VA; Gladka GV; Tashireva AA; Tashirev AB
[Ti] Título:[Taxonomical status of the psychrotolerant Antarctic microorganisms].
[So] Source:Mikrobiol Z;75(6):3-9, 2013 Nov-Dec.
[Is] ISSN:1028-0987
[Cp] País de publicação:Ukraine
[La] Idioma:rus
[Ab] Resumo:The aerobic chemoorganotrophic bacteria, dominating in soils and phytocenosis of the Antarctic Region, on combination of morphological and biochemical properties belong to several taxons of Bacteria domain. Gram-negative strains 3189, 3415 (fam. Halomonadaceae, Halomonas sp.) and 3088, 3468, 3469 (fam. Moraxellaceae, Psychrobacter sp.) belong to phylum Proteobacteria, to class Gammaproteobacteria. Gram-negative strains 3294 3392 (Rhizobiales, fam. Methylobacteriaceae, Methylobacterium sp.) relate to class Alphaproteobacteria of this phylum. Gram-positive strains 3179, 3275, 3470, 3471 (fam. Microbacteriaceae, Cryobacterium sp.), 3054, 3058, 3411 (fam. Corynebacteriaceae, Corynebacterium sp.) and 3194, 3398 (fam. Micrococcaceae, Micrococcus sp.) relate to phylum Actinobacteria, class Actinobacteria. Thus, the psychrophilic and psychrotolerant Antarctic bacteria (aerobic chemoorganotrophic) isolated from phytocenosis and soils of polar region are characterized by wide taxonomic variety.
[Mh] Termos MeSH primário: Actinomycetales/classificação
Halomonadaceae/classificação
Methylobacteriaceae/classificação
Moraxellaceae/classificação
Filogenia
Microbiologia do Solo
Microbiologia da Água
[Mh] Termos MeSH secundário: Actinomycetales/crescimento & desenvolvimento
Actinomycetales/metabolismo
Aerobiose
Regiões Antárticas
Temperatura Baixa
Meios de Cultura
Fermentação
Halomonadaceae/crescimento & desenvolvimento
Halomonadaceae/metabolismo
Methylobacteriaceae/crescimento & desenvolvimento
Methylobacteriaceae/metabolismo
Moraxellaceae/crescimento & desenvolvimento
Moraxellaceae/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:140123
[Lr] Data última revisão:
140123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140124
[St] Status:MEDLINE


  9 / 27 MEDLINE  
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[PMID]:24116094
[Au] Autor:O'Neill PK; Or M; Erill I
[Ad] Endereço:Department of Biological Sciences, University of Maryland Baltimore County (UMBC), Baltimore, Maryland, United States of America.
[Ti] Título:scnRCA: a novel method to detect consistent patterns of translational selection in mutationally-biased genomes.
[So] Source:PLoS One;8(10):e76177, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Codon usage bias (CUB) results from the complex interplay between translational selection and mutational biases. Current methods for CUB analysis apply heuristics to integrate both components, limiting the depth and scope of CUB analysis as a technique to probe into the evolution and optimization of protein-coding genes. Here we introduce a self-consistent CUB index (scnRCA) that incorporates implicit correction for mutational biases, facilitating exploration of the translational selection component of CUB. We validate this technique using gene expression data and we apply it to a detailed analysis of CUB in the Pseudomonadales. Our results illustrate how the selective enrichment of specific codons among highly expressed genes is preserved in the context of genome-wide shifts in codon frequencies, and how the balance between mutational and translational biases leads to varying definitions of codon optimality. We extend this analysis to other moderate and fast growing bacteria and we provide unified support for the hypothesis that C- and A-ending codons of two-box amino acids, and the U-ending codons of four-box amino acids, are systematically enriched among highly expressed genes across bacteria. The use of an unbiased estimator of CUB allows us to report for the first time that the signature of translational selection is strongly conserved in the Pseudomonadales in spite of drastic changes in genome composition, and extends well beyond the core set of highly optimized genes in each genome. We generalize these results to other moderate and fast growing bacteria, hinting at selection for a universal pattern of gene expression that is conserved and detectable in conserved patterns of codon usage bias.
[Mh] Termos MeSH primário: Códon
Mutação
Seleção Genética
[Mh] Termos MeSH secundário: Bases de Dados Genéticas
Genoma
Moraxellaceae/genética
Pseudomonas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Codon)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0076177


  10 / 27 MEDLINE  
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[PMID]:23832000
[Au] Autor:Petrova M; Shcherbatova N; Gorlenko Z; Mindlin S
[Ad] Endereço:Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq. 2, Moscow 123182, Russia. petrova@img.ras.ru
[Ti] Título:A new subgroup of the IS3 family and properties of its representative member ISPpy1.
[So] Source:Microbiology;159(Pt 9):1900-10, 2013 Sep.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, we described a novel insertion element, ISPpy1, isolated from a permafrost strain of Psychrobacter maritimus. In this work, we demonstrated that ISPpy1 is a member of a novel subgroup of the IS3 family of insertion sequences (ISs) that was not identified and characterized previously. IS elements of this subgroup termed the ISPpy1 subgroup are broadly distributed among different taxa of Eubacteria, including Geobacteraceae, Chlorobiaceae, Desulfobacteraceae, Methylobacteriaceae, Nitrosomonadaceae and Cyanobacteria. While displaying characteristic features of the IS3-family elements, ISPpy1 subgroup elements exhibit some unusual features. In particular, most of them have longer terminal repeats with unconventional ends and frameshifting box with an atypical organization, and, unlike many other IS3-family elements, do not exhibit any distinct IS specificity. We studied the transposition and mutagenic properties of a representative member of this subgroup, ISPpy1 and showed that in contrast to the original P. maritimus host, in a heterologous host, Escherichia coli K-12, it is able to translocate with extremely high efficiency into the chromosome, either by itself or as a part of a composite transposon containing two ISPpy1 copies. The majority of transposants carry multiple chromosomal copies (up to 12) of ISPpy1. It was discovered that ISPpy1 is characterized by a marked mutagenic activity in E. coli: its chromosomal insertions generate various types of mutations, including auxotrophic, pleiotropic and rifampicin-resistance mutations. The distribution of IS elements of the novel subgroup among different bacteria, their role in the formation of composite transposons and the horizontal transfer of genes are examined and discussed.
[Mh] Termos MeSH primário: Bactérias/genética
Elementos de DNA Transponíveis
Família Multigênica
[Mh] Termos MeSH secundário: Bactérias/classificação
Sequência de Bases
Escherichia coli/genética
Dosagem de Genes
Dados de Sequência Molecular
Moraxellaceae/classificação
Moraxellaceae/genética
Mutagênese Insercional
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Transposable Elements)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:130902
[Lr] Data última revisão:
130902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130709
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.068676-0



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