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Pesquisa : B03.440.400.425.550 [Categoria DeCS]
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[PMID]:28671534
[Au] Autor:Minegishi H; Enomoto S; Echigo A; Shimane Y; Kondo Y; Inoma A; Kamekura M; Takai K; Itoh T; Ohkuma M; Ihara K; Takahashi-Ando N; Fukushima Y; Ishii S; Yoshida Y; Usami R
[Ad] Endereço:3​Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan 2​Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan 1​Faculty of Science and Engineering, Toyo University, 2100 Kuj
[Ti] Título:Salinarchaeum chitinilyticum sp. nov., a chitin-degrading haloarchaeon isolated from commercial salt.
[So] Source:Int J Syst Evol Microbiol;67(7):2274-2278, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two chitin-degrading halophilic archaeal strains, MC-74T and MC-23, were isolated from commercial salt samples. Cells were motile, rod-shaped and stained Gram-negative. Colonies were vermillion-pigmented. Strains MC-74T and MC-23 were able to grow with 1.5-5.1 M NaCl (optimum, 2.6-3.1 M) at pH 6.0-10.0 (optimum, pH 7.0) and at 20-50 °C (optimum, 40 °C). The orthologous 16S rRNA gene sequence similarity between the two strains was 99.8 %, and the closest phylogenetic relative was Salinarchaeum laminariae JCM 17267T with 99.3-99.5 % similarity. The level of DNA-DNA relatedness between the two strains was 93 and 94 % (reciprocally), and those between the two strains and Salinarchaeumlaminariae JCM 17267T were 35-36 % and 38-39 % (reciprocally). The polar lipids of both strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Glycolipids were not detected. Based on the phenotypic and phylogenetic analyses, the strains represent a novel species of the genus Salinarchaeum, for which the name Salinarchaeum chitinilyticum sp. nov. is proposed. The type strain is MC-74T (=JCM 19597T=KCTC 4262T), isolated from solar salt produced in France. Strain MC-23, isolated from a commercial solar salt sample produced in China, is an additional strain of the species.
[Mh] Termos MeSH primário: Halobacteriaceae/classificação
Filogenia
Cloreto de Sódio/análise
[Mh] Termos MeSH secundário: China
Quitina/metabolismo
DNA Arqueal/genética
França
Glicolipídeos/química
Halobacteriaceae/genética
Halobacteriaceae/isolamento & purificação
Neisseriaceae/genética
Hibridização de Ácido Nucleico
Fosfatidilgliceróis/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Glycolipids); 0 (PGP-Me); 0 (Phosphatidylglycerols); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1398-61-4 (Chitin); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001941


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[PMID]:28406911
[Au] Autor:Li Y; Xue H; Sang SQ; Lin CL; Wang XZ
[Ad] Endereço:The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing, China.
[Ti] Título:Phylogenetic analysis of family Neisseriaceae based on genome sequences and description of Populibacter corticis gen. nov., sp. nov., a member of the family Neisseriaceae, isolated from symptomatic bark of Populus × euramericana canker.
[So] Source:PLoS One;12(4):e0174506, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two Gram-stain negative aerobic bacterial strains were isolated from the bark tissue of Populus × euramericana. The novel isolates were investigated using a polyphasic approach including 16S rRNA gene sequencing, genome sequencing, average nucleotide identity (ANI) and both phenotypic and chemotaxonomic assays. The genome core gene sequence and 16S rRNA gene phylogenies suggest that the novel isolates are different from the genera Snodgrassella and Stenoxybacter. Additionally, the ANI, G+C content, main fatty acids and phospholipid profile data supported the distinctiveness of the novel strain from genus Snodgrassella. Therefore, based on the data presented, the strains constitute a novel species of a novel genus within the family Neisseriaceae, for which the name Populibacter corticis gen. nov., sp. nov. is proposed. The type strain is 15-3-5T (= CFCC 13594T = KCTC 42251T).
[Mh] Termos MeSH primário: Genoma Bacteriano/fisiologia
Neisseriaceae/genética
Filogenia
Casca de Planta/microbiologia
Populus/microbiologia
[Mh] Termos MeSH secundário: Neisseriaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174506


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[PMID]:28161435
[Au] Autor:Wang Z; Zhu J; Liu Z; Liu Y; Zheng N; Feng M; Chen Q; Yu W; Jiang L; Hu J
[Ad] Endereço:Department of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.
[Ti] Título:Multi-locus sequence typing of Laribacter hongkongensis isolates from freshwater animals, environment and diarrhea patients in southern China.
[So] Source:Int J Food Microbiol;245:98-104, 2017 Mar 20.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Laribacter hongkongensis is a novel emerging bacterium associated with gastroenteritis and invasive bacteremic infections. Freshwater fish and edible frogs have been identified as major reservoirs of L. hongkongensis. Currently one of the main challenges in L. hongkongensis research is to identify their sources and possible transmission routes. The aim of this study was to determine the genetic diversity and relatedness of these L. hongkongensis isolates to their hosts in the hope of shedding light on these issues. In this study, multi-locus sequence typing (MLST) was used to determine the genetic characteristics of 114 L. hongkongensis strains, including 113 isolated from humans, fish, frogs, Amazonian snails and water sample in Guangzhou and Jiangmen, Southern China, and one reference isolate HZ242, recovered from a diarrhea patient in Hangzhou. The relationships among the STs and the relatedness among the isolates were assessed by phylogenetic and eBURST analysis. A total of 72 different sequence types (STs) from 114 isolates of L. hongkongensis were identified by MLST analysis, and ST99-ST161were novel. Significant difference of the prevalence of different STs between fish isolates (41.8%, 23/55) and frog isolates (82.4%, 42/51) was revealed (p=0.000). The most frequent ST (ST45) was identified 28 times and only found in fish isolates. In addition, 10 groups were identified by eBURST in this study. Combined the MLST data from Hong Kong and the present study, there were eight eBRUST lineages (group A-H) included the isolates (49.2%, 128/260) from either numerous hosts or multiple geographic origins, which contained 33.1% (53/160) of all the STs. Group A (n=57, STs=20) consisted exclusively of isolates from fish and 92.9% (39/42) of isolates in group B (n=42, STs=16) were only from fish. Group C-F (n=22, STs=14) were found to be associated with human, apart from other hosts. In this study, extensive genetic heterogeneity among the L. hongkongensis isolates from various hosts was observed. Specifically, there is higher genetic diversity of L. hongkongensis isolates of frog-origin than those of fish-origin. This study indicated some isolates exhibited a preference for specific hosts or geographic areas. ST45 was revealed to be the most frequent ST, which was only found in the fish isolates in Southern China, but might be irrelative to human infection. This MLST study further revealed that frog was likely to be another major source for human infection with L. hongkongensis apart from fish.
[Mh] Termos MeSH primário: Diarreia/microbiologia
Peixes/microbiologia
Água Doce/microbiologia
Neisseriaceae/classificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
China
Biologia Computacional
Gastroenterite/microbiologia
Seres Humanos
Tipagem de Sequências Multilocus
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170206
[St] Status:MEDLINE


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[PMID]:28085929
[Au] Autor:Kong HK; Law HW; Liu X; Law CO; Pan Q; Gao L; Xiong L; Lau SK; Woo PC; Lau TC
[Ad] Endereço:Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region, China.
[Ti] Título:Transcriptomic Analysis of Laribacter hongkongensis Reveals Adaptive Response Coupled with Temperature.
[So] Source:PLoS One;12(1):e0169998, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial adaptation to different hosts requires transcriptomic alteration in response to the environmental conditions. Laribacter hongkongensis is a gram-negative, facultative anaerobic, urease-positive bacillus caused infections in liver cirrhosis patients and community-acquired gastroenteritis. It was also found in intestine from commonly consumed freshwater fishes and drinking water reservoirs. Since L. hongkongensis could survive as either fish or human pathogens, their survival mechanisms in two different habitats should be temperature-regulated and highly complex. Therefore, we performed transcriptomic analysis of L. hongkongensis at body temperatures of fish and human in order to elucidate the versatile adaptation mechanisms coupled with the temperatures. We identified numerous novel temperature-induced pathways involved in host pathogenesis, in addition to the shift of metabolic equilibriums and overexpression of stress-related proteins. Moreover, these pathways form a network that can be activated at a particular temperature, and change the physiology of the bacteria to adapt to the environments. In summary, the dynamic of transcriptomes in L. hongkongensis provides versatile strategies for the bacterial survival at different habitats and this alteration prepares the bacterium for the challenge of host immunity.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Biomarcadores/metabolismo
Gastroenterite/genética
Genoma Bacteriano
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Neisseriaceae/genética
[Mh] Termos MeSH secundário: Gastroenterite/microbiologia
Seres Humanos
Neisseriaceae/isolamento & purificação
RNA Bacteriano/genética
Estresse Fisiológico
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (RNA, Bacterial)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169998


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[PMID]:27915412
[Au] Autor:Day PA; Villalba MS; Herrero OM; Arancibia LA; Alvarez HM
[Ad] Endereço:Instituto de Biociencias de La Patagonia (INBIOP), Universidad Nacional de La Patagonia San Juan Bosco Y CONICET, Km 4-Ciudad Universitaria, 9000, Comodoro Rivadavia, Chubut, Argentina.
[Ti] Título:Formation of indigoidine derived-pigments contributes to the adaptation of Vogesella sp. strain EB to cold aquatic iron-oxidizing environments.
[So] Source:Antonie Van Leeuwenhoek;110(3):415-428, 2017 Mar.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We investigated previously under explored cold aquatic environments of Andean Patagonia, Argentina. Oily sheens similar to an oil spill are frequently observed at the surface of water in creeks and small ponds in these places. Chemical analysis of a water sample revealed the occurrence of high concentrations of iron and the presence of a free insoluble indigoidine-derived pigment. A blue pigment-producing bacterium (strain EB) was isolated from the water sample and identified as Vogesella sp. by molecular analysis. The isolate was able to produce indigoidine and another derived-pigment (here called cryoindigoidine) with strong antifreeze properties. The production of the pigments depended on the cell growth at cold temperatures (below 15 °C), as well as on the attachment of cells to solid surfaces, and iron limitation in the media. The pigments produced by strain EB showed an inhibitory effect on the growth of diverse microorganisms such as Candida albicans, Escherichia coli and Staphylococcus aureus. In addition, pigmented cells were more tolerant to freezing than non-pigmented cells, suggesting a role of cryoindigoidine/indigoidine as a cold-protectant molecule. The possible roles of the pigments in strain EB physiology and its interactions with the iron-rich environment from which the isolate was obtained are discussed. Results of this study suggested an active role of strain EB in the investigated iron-oxidizing ecosystem.
[Mh] Termos MeSH primário: Ferro/metabolismo
Neisseriaceae/fisiologia
Pigmentos Biológicos/metabolismo
Piperidonas/metabolismo
[Mh] Termos MeSH secundário: Aclimatação
Argentina
Candida albicans/efeitos dos fármacos
Temperatura Baixa
Microbiologia Ambiental
Escherichia coli/efeitos dos fármacos
Congelamento
Água Doce/química
Água Doce/microbiologia
Neisseriaceae/genética
Neisseriaceae/isolamento & purificação
Neisseriaceae/metabolismo
Filogenia
Pigmentos Biológicos/química
Piperidonas/química
Tanques/química
Tanques/microbiologia
RNA Ribossômico 16S/genética
Staphylococcus aureus/efeitos dos fármacos
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pigments, Biological); 0 (Piperidones); 0 (RNA, Ribosomal, 16S); 2435-59-8 (indigoidine); E1UOL152H7 (Iron)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-016-0814-2


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[PMID]:27666417
[Au] Autor:Subhash Y; Park MJ; Lee SS
[Ad] Endereço:Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui- dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
[Ti] Título:Microvirgula curvata sp. nov., isolated from hydrocarbon-contaminated soil, and emended description of the genus Microvirgula.
[So] Source:Int J Syst Evol Microbiol;66(12):5309-5313, 2016 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel Gram-stain-negative, small curved-rod-shaped, motile strain, designated L6T, was isolated from hydrocarbon-contaminated soils collected from Kuwait. Strain L6T was able to grow at 10-40 °C (optimum, 27-32 °C), pH 6.1-8.8 (optimum, 6.5-7.5) and 0-4.5 % (w/v) NaCl (optimum, 0-0.5). C18 : 1ω6c/C18 : 1ω7c, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C12 : 0 and C12 : 0 3-OH were predominant fatty acids with minor amounts of C14 : 0 and C17 : 0 cyclo. Phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic G+C content was 61.2 mol%. 16S rRNA gene sequence comparisons indicated that strain L6T represents a member of the genus Microvirgula within the family Neisseriaceae of the class Betaproteobacteria. Strain L6T has a sequence similarity of 99.2 % with Microvirgula aerodenitrificans SGLY2T and <93.8 % with other members of the family Neisseriaceae. However, strain L6T showed only 56.5±2 % relatedness (based on DNA-DNA hybridization) with M. aerodenitrificans KACC 12055T (=SGLY2T). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain L6T as a representative of a novel species in the genus Microvirgula, for which the name Microvirgula curvata sp. nov. is proposed. The type strain is L6T (=KEMB 2255-471T=JCM 31223T). An emended description of the genus Microvirgula is also proposed.
[Mh] Termos MeSH primário: Poluição Ambiental
Hidrocarbonetos/análise
Neisseriaceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Kuweit
Neisseriaceae/genética
Neisseriaceae/isolamento & purificação
Hibridização de Ácido Nucleico
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Hydrocarbons); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 39382-08-6 (phosphatidylethanolamine); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001512


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[PMID]:27468990
[Au] Autor:Sheu SY; Li YS; Young CC; Chen WM
[Ad] Endereço:1​Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
[Ti] Título:Chitinibacter fontanus sp. nov., isolated from a spring.
[So] Source:Int J Syst Evol Microbiol;66(10):4262-4268, 2016 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain, designated STM-7T, was isolated from a spring in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain STM-7T were Gram-staining-negative, aerobic, poly-ß-hydroxybutyrate-accumulating, motile by a single polar flagellum, rod-shaped, surrounded by a thick capsule and formed milky-white colonies. Growth occurred at 15-37 °C (optimum, 25-30 °C), at pH 6-8 (optimum, pH 6-7) and with 0-2 % NaCl (optimum, 0-1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain STM-7T belonged to the genus Chitinibacter and was most closely related to Chitinibacter tainanensis S1T with a sequence similarity of 97.3 %. Strain STM-7T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the predominant fatty acids. The major hydroxyl fatty acids were C12 : 0 3-OH and C16 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized glycolipid and an uncharacterized phospholipid. The major isoprenoid quinone was Q-8. The DNA G+C content of the genomic DNA was 52.4 mol%. The DNA-DNA hybridization value for strain STM-7T with Chitinibacter tainanensis BCRC 17254T was less than 47 %. On the basis of the phylogenetic inference and phenotypic data, strain STM-7T should be classified as a representative of a novel species, for which the name Chitinibacter fontanus sp. nov. is proposed. The type strain is STM-7T (=BCRC 80923T=LMG 29289T=KCTC 42982T).
[Mh] Termos MeSH primário: Nascentes Naturais/microbiologia
Neisseriaceae/classificação
Filogenia
Microbiologia da Água
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hidroxibutiratos/química
Hidroxibutiratos/metabolismo
Neisseriaceae/genética
Neisseriaceae/isolamento & purificação
Hibridização de Ácido Nucleico
Fosfolipídeos/química
Poliésteres/metabolismo
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Taiwan
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Hydroxybutyrates); 0 (Phospholipids); 0 (Polyesters); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 26063-00-3 (poly-beta-hydroxybutyrate); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001345


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[PMID]:27246535
[Au] Autor:Zhang A; Gao C; Chen K; Wei C; Ouyang P
[Ad] Endereço:College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University.
[Ti] Título:Enhanced chitinase production by Chitinolyticbacter meiyuanensis SYBC-H1 using staged pH control.
[So] Source:J Gen Appl Microbiol;62(3):126-31, 2016 Jul 14.
[Is] ISSN:1349-8037
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The pH of a microbiological culture is important for both cell growth and chitinase accumulation, but the optimal pH is not normally the same for both. The objective of this study was to investigate the effect of pH on chitinase production by Chitinolyticbacter meiyuanensis strain SYBC-H1 (ATCC BAA-2140) in a mineral medium. The results of batch culture at different pH values showed that the optimum pH for cell growth and chitinase production varied with time, although KOH produced the best results for cell growth and chitinase production, NaOH was chosen because of cost considerations. We designed a three-stage pH control strategy using NaOH as the neutralizing agent. Maximum cell growth (1.07 g dry cell weight/l) and maximum chitinase activity (13.6 U/ml) were observed after culture at 26°C for 72 h in a mineral medium. These values were greater by 129% and 162%, respectively, and the length of time to attain maximum chitinase activity was decreased by 12 h, compared with results from an earlier study (Hao et al., 2011b).
[Mh] Termos MeSH primário: Técnicas Bacteriológicas
Quitina/metabolismo
Quitinases/biossíntese
Neisseriaceae/enzimologia
[Mh] Termos MeSH secundário: Meios de Cultura/química
Fermentação
Concentração de Íons de Hidrogênio
Hidróxidos/farmacologia
Neisseriaceae/crescimento & desenvolvimento
Neisseriaceae/isolamento & purificação
Compostos de Potássio/farmacologia
Hidróxido de Sódio/farmacologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Hydroxides); 0 (Potassium Compounds); 1398-61-4 (Chitin); 55X04QC32I (Sodium Hydroxide); EC 3.2.1.14 (Chitinases); WZH3C48M4T (potassium hydroxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.2323/jgam.2016.01.003


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[PMID]:27240345
[Au] Autor:Hao Z; Wu H; Yang M; Chen J; Xi L; Zhao W; Yu J; Liu J; Liao X; Huang Q
[Ad] Endereço:Institute of Applied Biotechnology, Taizhou Vocational & Technical College, Taizhou 318000, China. haozhikui123@126.com.
[Ti] Título:Cloning, Expression and 3D Structure Prediction of Chitinase from Chitinolyticbacter meiyuanensis SYBC-H1.
[So] Source:Int J Mol Sci;17(6), 2016 May 26.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Two CHI genes from Chitinolyticbacter meiyuanensis SYBC-H1 encoding chitinases were identified and their protein 3D structures were predicted. According to the amino acid sequence alignment, CHI1 gene encoding 166 aa had a structural domain similar to the GH18 type II chitinase, and CHI2 gene encoding 383 aa had the same catalytic domain as the glycoside hydrolase family 19 chitinase. In this study, CHI2 chitinase were expressed in Escherichia coli BL21 cells, and this protein was purified by ammonium sulfate precipitation, DEAE-cellulose, and Sephadex G-100 chromatography. Optimal activity of CHI2 chitinase occurred at a temperature of 40 °C and a pH of 6.5. The presence of metal ions Fe(3+), Fe(2+), and Zn(2+) inhibited CHI2 chitinase activity, while Na⁺ and K⁺ promoted its activity. Furthermore, the presence of EGTA, EDTA, and ß-mercaptoethanol significantly increased the stability of CHI2 chitinase. The CHI2 chitinase was active with p-NP-GlcNAc, with the Km and Vm values of 23.0 µmol/L and 9.1 mM/min at a temperature of 37 °C, respectively. Additionally, the CHI2 chitinase was characterized as an N-acetyl glucosaminidase based on the hydrolysate from chitin. Overall, our results demonstrated CHI2 chitinase with remarkable biochemical properties is suitable for bioconversion of chitin waste.
[Mh] Termos MeSH primário: Quitinases/química
Quitinases/genética
Clonagem Molecular/métodos
Neisseriaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Quitinases/metabolismo
Escherichia coli/genética
Cinética
Modelos Moleculares
Neisseriaceae/química
Neisseriaceae/enzimologia
Neisseriaceae/genética
Filogenia
Estabilidade Proteica
Estrutura Secundária de Proteína
Análise de Sequência de Proteína
Microbiologia do Solo
Homologia Estrutural de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 3.2.1.14 (Chitinases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160531
[St] Status:MEDLINE


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[PMID]:27180655
[Au] Autor:Martin H; Laborel-Préneron E; Fraysse F; Nguyen T; Schmitt AM; Redoulès D; Davrinche C
[Ad] Endereço:a INSERM UMR 1043, CNRS UMR 5282, Université Toulouse III Paul Sabatier , Toulouse , France.
[Ti] Título:Aquaphilus dolomiae extract counteracts the effects of cutaneous S. aureus secretome isolated from atopic children on CD4 T cell activation.
[So] Source:Pharm Biol;54(11):2782-2785, 2016 Nov.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Skin microbiota takes part in the control of cutaneous inflammation. In skin diseases such as atopic dermatitis (AD) cutaneous dysbiosis and the emergence of Staphylococcus aureus contribute to the pathophysiology of the disease. New therapeutic approaches consist in topical application of natural products able to counteract S. aureus effects through activation of resident immune cells producing anti-inflammatory cytokines such as IL-10. OBJECTIVE: This study investigates the potential immunosuppressive properties of Aquaphilus dolomiae (Neisseriaceae), a flagellated bacterium contained in Avène Thermal Spring Water used in hydrotherapy treatments of AD patients. MATERIALS AND METHODS: An aqueous protein extract of Aquaphilus dolomiae (ADE, 60 µg/mL) was added to human monocyte-derived dendritic cells (moDC) for 24 h. Expression of HLA-DR, CD86 and CD83 was evaluated by flow cytometry and released cytokines (IL-10, IL-12) by cytometry bead array assay. The proliferation of allogeneic CFSE-labelled CD4 T cells stimulated with ADE-conditioned moDC and S. aureus secretome was analysed by flow cytometry. RESULTS: MoDC exposed to ADE expressed lower levels of HLA-DR and CD86 than untreated cells, no CD83 and secreted barely detectable IL-12 but high amounts of IL-10 (N = 12, p < 0.0002). The proliferative effect of S. aureus secretome on CD4 T cells was reduced (p < 0.001) in the presence of ADE-moDC. CONCLUSION: ADE counteracted the mitogenic effect of a S. aureus secretome on CD4 T cells. Owing to the role of S. aureus colonization in driving inflammation in AD the immunosuppressive property of the ADE might be useful to reduce disease severity.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Dermatite Atópica/tratamento farmacológico
Imunossupressores/farmacologia
Ativação Linfocitária
Neisseriaceae
Extratos Vegetais/farmacologia
Pele/microbiologia
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Criança
Dermatite Atópica/microbiologia
Seres Humanos
Interleucina-10/biossíntese
Staphylococcus aureus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunosuppressive Agents); 0 (Plant Extracts); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160517
[St] Status:MEDLINE



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