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  1 / 176 MEDLINE  
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[PMID]:28463567
[Au] Autor:Tirado-Torres D; Acevedo-Sandoval O; Rodríguez-Pastrana BR; Gayosso-Canales M
[Ad] Endereço:a Institute of Basic Sciences and Engineering, Autonomous University of Hidalgo (UAEH) , Pahuca, Hidalgo , Mexico.
[Ti] Título:Phylogeny and polycyclic aromatic hydrocarbons degradation potential of bacteria isolated from crude oil-contaminated site.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;52(9):897-904, 2017 Jul 29.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study employed the use of 16S rRNA gene sequence analysis to identify three of four native bacterial strains isolated from crude oil-contaminated site in Poza Rica, Veracruz, Mexico. The identified bacteria were Ochrobactrum intermedium, Pandoraea pnomenusa and Ochrobactrum sp., but SA2-09 strain was not identified. The ability of the isolates to degrade polycyclic aromatic hydrocarbons (PAHs) was evaluated at 31.61 and 54.52 mg/kg PAHs in soil, when used as crude oil in soil microcosm during 80 days of incubation at 30°C. The results demonstrated that O. intermedium biodegraded many PAHs, including the high molecular weight (HMW) PAHs fluoranthene (100% equivalent 0.24 mg/kg), benzo [b] fluoranthene (81.8% equal 0.18 mg/kg), Benzo[a]pyrene (87.0%, 0.20 mg/kg) and Benzo[g,h,i]perylene (52.7%, 0.39 mg/kg). P. pnomenusa had a degradation profile of HMW PAHs, which was similar to O. intermedium, while Ochrobactrum sp. and the strain SA-09 exhibited lower degradation rates of HMW.
[Mh] Termos MeSH primário: Burkholderia/isolamento & purificação
Ochrobactrum/isolamento & purificação
Petróleo/análise
Hidrocarbonetos Aromáticos Policíclicos/análise
Poluentes do Solo/análise
[Mh] Termos MeSH secundário: Biodegradação Ambiental
México
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Petroleum); 0 (Polycyclic Aromatic Hydrocarbons); 0 (RNA, Ribosomal, 16S); 0 (Soil Pollutants)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2017.1316170


  2 / 176 MEDLINE  
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[PMID]:28229216
[Au] Autor:Yun H; Liang B; Kong D; Li Z; Qi G; Wang A
[Ad] Endereço:Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
[Ti] Título:Enhanced Biotransformation of Triclocarban by Ochrobactrum sp. TCC-1 Under Anoxic Nitrate Respiration Conditions.
[So] Source:Curr Microbiol;74(4):491-498, 2017 Apr.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antimicrobial triclocarban (3,4,4'-trichlorocarbanilide, TCC) is frequently detected in soils and sediments for the widely reclaim of sewage sludge or biosolid in recent decades. This resulted from a weak removal of TCC during wastewater treatment, and most of it adsorbed onto sewage sludge. As the toxicity and persistence of TCC in the environment, the elimination of TCC from the source of output is of great importance, particularly in anoxic process. In this study, the biotransformation of TCC by a newly isolated TCC-degrading strain Ochrobactrum sp. TCC-1 under anoxic conditions was investigated. By testing different carbon nitrogen ratios (C/N), it showed that nitrate could support the growth of strain TCC-1 and enhance the hydrolysis of TCC to more biodegradable chloroanilines, especially with a higher C/N of 10 and under anaerobic conditions. In wastewater sewage sludge, strain TCC-1 colonized and maintained the TCC-hydrolyzing activity under the nitrate respiration mode. These results would lay a basic foundation for the potential bioremediation of TCC-contaminated anoxic sites with TCC-degrading strain.
[Mh] Termos MeSH primário: Carbanilidas/metabolismo
Nitratos/metabolismo
Ochrobactrum/metabolismo
[Mh] Termos MeSH secundário: Carbono/metabolismo
Hidrólise
Nitrogênio/metabolismo
Esgotos/microbiologia
Eliminação de Resíduos Líquidos/métodos
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbanilides); 0 (Nitrates); 0 (Sewage); 7440-44-0 (Carbon); BGG1Y1ED0Y (triclocarban); N762921K75 (Nitrogen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-017-1214-1


  3 / 176 MEDLINE  
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[PMID]:28050740
[Au] Autor:Yang L; Ying C; Fang N; Zhong Y; Zhao-Xiang Z; Yun S
[Ad] Endereço:Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China.
[Ti] Título:Identification and Characterization of a High Efficiency Aniline Resistance and Degrading Bacterium MC-01.
[So] Source:Appl Biochem Biotechnol;182(1):41-54, 2017 May.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biodegradation is one of the important methods for the treatment of industrial wastewater containing aniline. In this paper, a degrading bacterium named MC-01, which could survive in high concentration aniline wastewater, was screened from industrial wastewater containing aniline and sludge. MC-01 was preliminarily identified as Ochrobactrum sp. based on the amplified 16S rDNA gene sequence and Biolog system identification. MC-01 was highly resistant to aniline. After 24-h culture under aniline concentration of 6500 mg/L, the amount of bacterium survived still remained 0.05 × 10 CFU/mL. Experiments showed that there was no coupling expression between the growth of MC-01 and aniline degradation. The optimum growth conditions in LB culture were pH 6.0, 30 °C of temperature, and 4% of incubation amount, respectively. And the optimum conditions of aniline degradation of MC-01 were pH 7.0, 45 °C of temperature, and 3.0% of salt concentration, respectively. The degradation rate of MC-01 (48 h) in different aniline concentrations (200~1600 mg/L) was stable under the optimum conditions, which could reach more than 75%.
[Mh] Termos MeSH primário: Compostos de Anilina/metabolismo
Ochrobactrum/isolamento & purificação
RNA Ribossômico 16S/genética
Águas Residuais/microbiologia
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Seres Humanos
Concentração de Íons de Hidrogênio
Resíduos Industriais
Ochrobactrum/classificação
Ochrobactrum/genética
Ochrobactrum/metabolismo
Filogenia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Industrial Waste); 0 (RNA, Ribosomal, 16S); 0 (Waste Water); 0 (Water Pollutants, Chemical); SIR7XX2F1K (aniline)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2309-z


  4 / 176 MEDLINE  
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[PMID]:27995689
[Au] Autor:Yu X; Li Y; Cui Y; Liu R; Li Y; Chen Q; Gu Y; Zhao K; Xiang Q; Xu K; Zhang X
[Ad] Endereço:College of Resources, Sichuan Agricultural University, Chengdu, China.
[Ti] Título:An indoleacetic acid-producing Ochrobactrum sp. MGJ11 counteracts cadmium effect on soybean by promoting plant growth.
[So] Source:J Appl Microbiol;122(4):987-996, 2017 Apr.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To analyse whether some indoleacetic acid (IAA)-secreting plant growth-promoting bacteria can alleviate cadmium (Cd) stress, the role of an Ochrobactrum sp. MGJ11 from rhizosphere of soybean in promoting plant growth, and to evaluate the counteracting Cd effects on soybean. METHODS AND RESULTS: Ochrobactrum sp. MGJ11 produced 121·2 mg l of IAA. MGJ11 increased soybean root length, shoot length and biomass by 30·1, 30·8 and 13·4% respectively. In liquid medium, no IAA production was detected in Cd concentration of 100 mg l . In soil with 20-80 mg kg Cd, MGJ11 promoted soybean root elongation (29·4-161·4%) and increased the shoot length (up to 52·7%) and biomass (up to 87·2%). After growing for 38 days, Cd concentrations in the roots of inoculated soybean were lower than in those of noninoculated plants. Only a little Cd (2·6-16·9 µg g ) was translocated from the root to shoot. CONCLUSIONS: Ochrobactrum sp. MGJ11 secretes IAA and shows tolerance against Cd. MGJ11 inoculation improves the root length, shoot length and biomass of soybean in both vermiculite and Cd vermiculite, and decreases Cd concentration of soybean root. The characteristics of MGJ11 suggest that it could be used for promoting soybean growth and lowering bioavailability of soil Cd for soybean root. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, we isolated a plant growth-promoting Ochrobactrum with the activity of mitigating Cd toxicity to plant roots. The Ochrobactrum can be considered as a potential bioaugmentation agent that promotes plant growth, especially in some agricultural systems, or that helps in the phytoremediation of Cd-contaminated soil.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Ácidos Indolacéticos/metabolismo
Ochrobactrum/metabolismo
Poluentes do Solo/toxicidade
Feijão de Soja/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Biomassa
Cádmio/metabolismo
Raízes de Plantas/metabolismo
Rizosfera
Solo/química
Poluentes do Solo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Soil); 0 (Soil Pollutants); 00BH33GNGH (Cadmium); 6U1S09C61L (indoleacetic acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13379


  5 / 176 MEDLINE  
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[PMID]:27966913
[Au] Autor:Yun H; Liang B; Qiu J; Zhang L; Zhao Y; Jiang J; Wang A
[Ad] Endereço:Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing, 100085, China.
[Ti] Título:Functional Characterization of a Novel Amidase Involved in Biotransformation of Triclocarban and its Dehalogenated Congeners in Ochrobactrum sp. TCC-2.
[So] Source:Environ Sci Technol;51(1):291-300, 2017 Jan 03.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Haloaromatic antimicrobial triclocarban (3,4,4'-trichlorocarbanilide, TCC) is a refractory contaminant which is frequently detected in various aquatic and sediment environments globally. However, few TCC-degrading communities or pure cultures have been documented, and functional enzymes involved in TCC biodegradation hitherto have not yet been characterized. In this study, a bacterial strain, Ochrobactrum sp. TCC-2, capable of degrading TCC under both aerobic and anaerobic conditions was isolated from a sediment sample. A novel amidase gene (tccA), responsible for the hydrolysis of the two amide bonds of TCC and its dehalogenated congeners 4,4'-dichlorocarbanilide (DCC) and carbanilide (NCC) to more biodegradable chloroaniline or aniline products, was cloned and characterized. TccA shares low amino acid sequence identity (27 to 38%) with other biochemically characterized amidases and contains the conserved catalytic triad (Ser-Ser-Lys) of the amidase signature enzyme family. TccA was stable over a pH range of 5.0 to 10.0 and at temperatures lower than 60 °C, and it was constitutively expressed in strain TCC-2. In contrast to the halogenated TCC and DCC, the nonchlorinated NCC was the preferred substrate for TccA. TccA also had hydrolysis activity to a broad spectrum of amide bonds in herbicides, insecticides, and chemical intermediates. The constitutive expression and broad substrate spectrum of TccA suggested strain TCC-2 may be potentially useful for bioremediation applications.
[Mh] Termos MeSH primário: Biotransformação
Ochrobactrum/metabolismo
[Mh] Termos MeSH secundário: Amidoidrolases/metabolismo
Biodegradação Ambiental
Hidrólise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.5.- (Amidohydrolases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.6b04885


  6 / 176 MEDLINE  
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[PMID]:27863881
[Au] Autor:Zhang W; Qiu L; Gong A; Yuan X
[Ad] Endereço:School of Chemistry and Biology Engineering, University of Science and Technology, Beijing 100083, China.
[Ti] Título:Isolation and characterization of a high-efficiency erythromycin A-degrading Ochrobactrum sp. strain.
[So] Source:Mar Pollut Bull;114(2):896-902, 2017 Jan 30.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this work, Erythromycin A(EA)- degrading bacteria was isolated from the contaminated soil obtained from a pharmaceutical factory in China. The isolate designated as strain WX-J1 was identified as Ochrobactrum sp. by sequence analysis of its 16S rDNA gene. It can grow in a medium containing EA as the sole source of carbon and its optimal growth pH and temperature were 6.5 and 32°C, respectively. Under these conditions, when the initial Erythromycin A concentration was 100mg·L , 97% of Erythromycin A has been degraded. HPLC-MS analyses indicated that Erythromycin A degradation produced intermediates contained the following three substances: 3-depyranosyloxy erythromycin A, 7,12-dyhydroxy-6-deoxyerythronolide B, 6-deoxyerythronolide B and propionaldehyde. Since Erythromycin A-degrading Ochrobactrum sp. strain rapidly degraded Erythromycin A, this strain might be useful for bioremediation purposes.
[Mh] Termos MeSH primário: Poluentes Ambientais/análise
Eritromicina/análise
Ochrobactrum/isolamento & purificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
China
DNA Ribossômico/genética
Poluentes Ambientais/química
Poluentes Ambientais/metabolismo
Eritromicina/química
Eritromicina/metabolismo
Estrutura Molecular
Ochrobactrum/metabolismo
RNA Ribossômico 16S/genética
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (Environmental Pollutants); 0 (RNA, Ribosomal, 16S); 63937KV33D (Erythromycin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE


  7 / 176 MEDLINE  
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[PMID]:27853952
[Au] Autor:Mishra SK; Khan MH; Misra S; Dixit VK; Khare P; Srivastava S; Chauhan PS
[Ad] Endereço:Division of Plant Microbe Interactions, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
[Ti] Título:Characterisation of Pseudomonas spp. and Ochrobactrum sp. isolated from volcanic soil.
[So] Source:Antonie Van Leeuwenhoek;110(2):253-270, 2017 Feb.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Soil bacteria may have properties of plant growth promotion but not be sufficiently beneficial for plants under stress conditions. This challenge has led researchers to extend their searches into extreme environments for potential soil bacteria with multiple plant beneficial traits as well as abiotic stress tolerance abilities. In the current study, an attempt was made to evaluate soil bacteria from an extreme environment, volcano soils, based on plant growth promoting and abiotic stress mitigating characteristics. The screening led to the isolation of eight (NBRISH4, NBRISH6, NBRISH10, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and NBRISH26) bacterial isolates capable of withstanding stresses, namely temperature (up to 45 °C), salt (up to 2 M NaCl) and drought (up to 60% Poly Ethylene Glycol 6000) in vitro. Further, the selected isolates were notable for their in vitro temporal performance with regards to survival (in terms of colony count), phosphate solubilisation, biofilm formation, auxin, alginate and exo-polysaccharide production abilities under abiotic stresses i.e. 40 °C temperature; 500 mM NaCl salt and drought (PEG) conditions. In vivo seed treatments of individual selected bacteria to maize plants resulted into significant enhancement in root and shoot length, root and shoot fresh and dry weight and number of leaves per plant. Overall, the plant growth promoting and abiotic stress tolerance ability was most evident for bacterial isolate NBRISH6 which was identified as an Ochrobactrum sp. using 16S rRNA based phylogenetic analysis.
[Mh] Termos MeSH primário: Ochrobactrum/classificação
Ochrobactrum/genética
Pseudomonas/classificação
Pseudomonas/genética
Microbiologia do Solo
[Mh] Termos MeSH secundário: Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-016-0796-0


  8 / 176 MEDLINE  
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[PMID]:27287730
[Au] Autor:Kulkarni G; Gohil K; Misra V; Kakrani AL; Misra SP; Patole M; Shouche Y; Dharne M
[Ad] Endereço:Molecular Biology Unit, National Centre for Cell Science, Pune, India.
[Ti] Título:Multilocus sequence typing of Ochrobactrum spp. isolated from gastric niche.
[So] Source:J Infect Public Health;10(2):201-210, 2017 Mar - Apr.
[Is] ISSN:1876-035X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The human stomach is colonized by diverse bacterial species. The presence of non-Helicobacter pylori bacteria in urease-positive biopsies of individuals has been reported. Bacteria belonging to the Ochrobactrum genus have been documented in the human gastric niche. The co-occurrence of Ochrobactrum spp. with H. pylori was previously reported in an antral biopsy of a non-ulcer dyspeptic (NUD) subject from Northern India. There is no information on the genetic diversity of Ochrobactrum spp. isolated from the gastric niche in the stomach. We aimed to study the species distribution and diversity of Ochrobactrum spp. with and without H. pylori in urease-positive biopsies across three different geographical regions in India. Sixty-two Ochrobactrum isolates recovered from patients with an upper gastric disorder (n=218) were subjected to molecular identification and multilocus sequence typing. H. pylori DNA was found in the majority of biopsies, which had a variable degree of Ochrobactrum spp present. Interestingly, some of the urease-positive biopsies only had Ochrobactrum without any H. pylori DNA. Based on phylogenetic analysis, the Ochrobactrum isolates were distributed into the O. intermedium, O. anthropi and O. oryzae groups. This indicates there are multiple species in the gastric niche irrespective of the presence or absence of H. pylori. Antibiotyping based on colistin and polymyxin B could differentiate between O. intermedium and O. anthropi without revealing the resistance-driven diversity. Considering the prevalence of multiple Ochrobactrum spp. in the human gastric niche, it is important to evaluate the commensal and/or pathogenic nature of non-H. pylori bacteria with respect to their geographical distribution, lifestyle and nutrition needs.
[Mh] Termos MeSH primário: Mucosa Gástrica/microbiologia
Gastrite/microbiologia
Variação Genética
Infecções por Bactérias Gram-Negativas/microbiologia
Tipagem de Sequências Multilocus
Ochrobactrum/classificação
Ochrobactrum/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Biópsia
Feminino
Genótipo
Helicobacter pylori/isolamento & purificação
Seres Humanos
Índia
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Ochrobactrum/isolamento & purificação
Filogenia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE


  9 / 176 MEDLINE  
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[PMID]:28036367
[Au] Autor:Scholz HC; Mühldorfer K; Shilton C; Benedict S; Whatmore AM; Blom J; Eisenberg T
[Ad] Endereço:Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, Munich and German Center for Infection Research (DZIF), Munich, Germany.
[Ti] Título:The Change of a Medically Important Genus: Worldwide Occurrence of Genetically Diverse Novel Brucella Species in Exotic Frogs.
[So] Source:PLoS One;11(12):e0168872, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genus Brucella comprises various species of both veterinary and human medical importance. All species are genetically highly related to each other, sharing intra-species average nucleotide identities (ANI) of > 99%. Infections occur among various warm-blooded animal species, marine mammals, and humans. Until recently, amphibians had not been recognized as a host for Brucella. In this study, however, we show that novel Brucella species are distributed among exotic frogs worldwide. Comparative recA gene analysis of 36 frog isolates from various continents and different frog species revealed an unexpected high genetic diversity, not observed among classical Brucella species. In phylogenetic reconstructions the isolates consequently formed various clusters and grouped together with atypical more distantly related brucellae, like B. inopinata, strain BO2, and Australian isolates from rodents, some of which were isolated as human pathogens. Of one frog isolate (10RB9215) the genome sequence was determined. Comparative genome analysis of this isolate and the classical Brucella species revealed additional genetic material, absent from classical Brucella species but present in Ochrobactrum, the closest genetic neighbor of Brucella, and in other soil associated genera of the Alphaproteobacteria. The presence of gene clusters encoding for additional metabolic functions, flanked by tRNAs and mobile genetic elements, as well as by bacteriophages is suggestive for a different ecology compared to classical Brucella species. Furthermore it suggests that amphibian isolates may represent a link between free living soil saprophytes and the pathogenic Brucella with a preferred intracellular habitat. We therefore assume that brucellae from frogs have a reservoir in soil and, in contrast to classical brucellae, undergo extensive horizontal gene transfer.
[Mh] Termos MeSH primário: Anuros/microbiologia
Brucella/genética
[Mh] Termos MeSH secundário: Animais
Austrália
Brucelose/microbiologia
DNA Bacteriano/genética
Transferência Genética Horizontal/genética
Variação Genética/genética
Seres Humanos
Ochrobactrum/genética
Filogenia
Roedores/microbiologia
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0168872


  10 / 176 MEDLINE  
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[PMID]:27479773
[Au] Autor:Liu T; Wei L; Qiao M; Zou D; Yang X; Lin A
[Ad] Endereço:Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
[Ti] Título:Mineralization of pyrene induced by interaction between Ochrobactrum sp. PW and ryegrass in spiked soil.
[So] Source:Ecotoxicol Environ Saf;133:290-6, 2016 Nov.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study was conducted to investigate the capability of pyrene-degrading bacterium Ochrobactrum sp. PW and ryegrass (Lolium multiflorum) grown alone and in combination on the degradation of pyrene in soil. After 60 days of ryegrass growth, plant biomass, pyrene-degrading microbial mass, soil enzyme activity (catalase activity and polyphenol oxidase activity) and residual concentration of pyrene in soils were determined. Higher dissipation rates were observed in PW inoculation treatments: ryegrass+PW rhizosphere soil (RP-r) and ryegrass+PW non-rhizosphere soil (RP-nr), than planting of ryegrass alone, rhizosphere (R-r) or non-rhizosphere (R-nr). The inoculation with PW significantly (p<0.05) increased the dry weight of ryegrass root and shoot, nearly 2.8 and 3.3 times higher than ryegrass treatment. The pyrene-degrading microbial mass indicated that a much larger mass of bacteria, actinobacteria were present in RP treatment. The catalase activity in all different treatments were significantly (p<0.05) higher than in with treatment R-nr, and the polyphenol oxidase activity was also significantly (p<0.05) increased by inoculation with PW, leading to enhanced mineralization of pyrene from soil. Our results suggest that adding of PAHs-degrading bacteria to soil can enhance remediation of PAHs contaminated soil, while improving plant growth.
[Mh] Termos MeSH primário: Lolium/metabolismo
Ochrobactrum/metabolismo
Pirenos/metabolismo
Poluentes do Solo/metabolismo
Solo/química
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Biomassa
Raízes de Plantas/metabolismo
Hidrocarbonetos Aromáticos Policíclicos
Pirenos/química
Microbiologia do Solo
Poluentes do Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polycyclic Aromatic Hydrocarbons); 0 (Pyrenes); 0 (Soil); 0 (Soil Pollutants)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE



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