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[PMID]:29198862
[Au] Autor:Sall C; Ayé M; Bottzeck O; Praud A; Blache Y
[Ad] Endereço:Laboratoire de chimie, UFR des Sciences de la Santé, Université de Thiès, BP 967 Thiès, Senegal.
[Ti] Título:Towards smart biocide-free anti-biofilm strategies: Click-based synthesis of cinnamide analogues as anti-biofilm compounds against marine bacteria.
[So] Source:Bioorg Med Chem Lett;28(2):155-159, 2018 01 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A set of triazole-based analogues of N-coumaroyltyramine was designed to discover potential leads that may help in the control of bacterial biofilms. the most potent compounds act as inhibitors of biofilm development with EC50 closed to ampicillin (EC50 = 11 µM) without toxic effect on bacterial growth even at high concentrations(100 µM).
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Ácidos Cumáricos/farmacologia
Paracoccus/efeitos dos fármacos
Pseudoalteromonas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/síntese química
Antibacterianos/química
Ácidos Cumáricos/síntese química
Ácidos Cumáricos/química
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Estrutura Molecular
Paracoccus/crescimento & desenvolvimento
Pseudoalteromonas/crescimento & desenvolvimento
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Coumaric Acids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


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[PMID]:28809137
[Au] Autor:Chen WM; Li YS; Young CC; Sheu SY
[Ad] Endereço:1​Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
[Ti] Título:Paracoccus mangrovi sp. nov., isolated from a mangrove.
[So] Source:Int J Syst Evol Microbiol;67(8):2689-2695, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain, designated gyp-1T, was isolated from a mangrove in Taiwan and characterized using the polyphasic taxonomic approach. Cells of gyp-1T were Gram-staining-negative, aerobic, poly-ß-hydroxybutyrate-accumulating, non-motile, coccoid or short-rod-shaped and formed cream-coloured colonies. Growth occurred at 15-37 °C (optimum, 25-30 °C), at pH 5.5-7.0 (optimum, pH 6.0) and with 0-4 % NaCl (optimum, 1-2 %). Phylogenetic analyses based on 16S rRNA gene sequences indicated that gyp-1T represented a member of the genus Paracoccus and showed the highest levels of sequence similarity with respect to Paracoccus lutimaris HDM-25T (97.8 %) and Paracoccus aminovorans DM-82T (97.7 %). The major fatty acids (>10 %) of gyp-1T were C18 : 1ω7c and C16 : 0. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, an unidentified glycolipid and two unidentified phospholipids. The major isoprenoid quinone was Q-10. The DNA G+C content was 64.6 mol%. The DNA-DNA hybridization value for gyp-1T with P. lutimaris HDM-25T and P. aminovorans DM-82T was less than 50 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that gyp-1T should be classified as representing a novel species of the genus Paracoccus, for which the name Paracoccus mangrovi sp. nov. is presented. The type strain is gyp-1T (=BCRC 80920T=LMG 29172T=KCTC 42899T).
[Mh] Termos MeSH primário: Avicennia/microbiologia
Paracoccus/classificação
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hidroxibutiratos/metabolismo
Hibridização de Ácido Nucleico
Paracoccus/genética
Paracoccus/isolamento & purificação
Fosfolipídeos/química
Poliésteres/metabolismo
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Taiwan
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Hydroxybutyrates); 0 (Phospholipids); 0 (Polyesters); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 26063-00-3 (poly-beta-hydroxybutyrate); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001993


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[PMID]:28758621
[Au] Autor:Xue H; Piao CG; Guo MW; Wang LF; Li Y
[Ad] Endereço:Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Dong xiao-fu NO. 1, Haidian District, Beijing 100091, PR China.
[Ti] Título:Paracoccus aerius sp. nov., isolated from air.
[So] Source:Int J Syst Evol Microbiol;67(8):2586-2591, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain 011410T, isolated from air at the foot of Xiangshan Mountain, Beijing, China, was Gram-reaction-negative, facultatively anaerobic, oval-shaped, motile with two flagella and catalase- and oxidase-positive. Growth of strain 011410T was observed at 4-41 °C (optimum, 30 °C), at pH 4.5-10.0 (optimum, pH 8.0) and at salinities of 0-10 % (w/v) NaCl (optimum, 0-2 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 011410T was a member of the genus Paracoccus and was related most closely to Paracoccus aestuarii B7T (96.62 % similarity) and Paracoccus sediminis CMB17T (96.48 % similarity). The major fatty acid was identified as C18 : 1ω7c, with smaller amounts of C18 : 0, C10 : 0 3-OH and summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I). The predominant respiratory quinone was ubiquinone-10 (Q-10), with Q-9 as a minor component. Polar lipid analysis indicated the presence of diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, five unknown phospholipids, one unknown aminolipid, one unknown glycolipid and two unknown polar lipids. The DNA G+C content of the strain was 63.5 mol%. On the basis of the data from this polyphasic characterization, strain 011410T represents a novel species, for which the name Paracoccus aerius sp. nov. is proposed. The type strain is 011410T (=CFCC 14285T=KCTC 42845T).
[Mh] Termos MeSH primário: Microbiologia do Ar
Paracoccus/classificação
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Pequim
DNA Bacteriano/genética
Ácidos Graxos/química
Paracoccus/genética
Paracoccus/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001976


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[PMID]:28598318
[Au] Autor:Yan ZF; Moya G; Lin P; Won KH; Yang JE; Li CT; Kook M; Wang QJ; Yi TH
[Ad] Endereço:1​Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
[Ti] Título:Paracoccus hibisci sp. nov., isolated from the rhizosphere of Hibiscus syriacus L. (Mugunghwa flower).
[So] Source:Int J Syst Evol Microbiol;67(6):1849-1854, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, aerobic, short-rod-shaped bacterium, motile by means of one flagellum (THG-T2.8T), was isolated from the rhizosphere of Mugunghwa flower. Growth occurred at 10-37 °C (optimum 28 °C), at pH 6-8 (optimum 7) and with 0-5 % NaCl (optimum 1 %). The major quinone was ubiquinone-10 (Q-10). The major fatty acids were C10 : 0 3-OH, C16 : 0, C18 : 0 and C18 : 1ω7c. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid, two unknown phospholipids, one unknown glycolipid and one unidentified lipid. The DNA G+C content of strain THG-T2.8T was 65.5 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.8T were identified as Paracoccus tibetensis Tibet-S9a3T (98.6 %), Paracoccus aestuarii B7T (98.4 %), Paracoccus rhizosphaerae CC-CCM15-8T (98.3 %) and Paracoccus beibuensis JLT1284T (98.2 %). Levels of sequence similarity among strain THG-T2.8T and other species of the genus Paracoccus were lower than 98.0 %. DNA-DNA hybridization values between strain THG-T2.8T and P. tibetensis Tibet-S9A3TT, P. aestuarii B7T, P. rhizosphaerae CC-CCM15-8T and P. beibuensisJLT1284T were 36.5 % (38.8 %, reciprocal analysis), 32.8 % (34.8 %), 31.6 % (33.8 %) and 15.3 % (24.8 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T2.8T represents a novel species of the genus Paracoccus, for which the name Paracoccus hibisci sp. nov. is proposed. The type strain is THG-T2.8T (=KACC 18932T=CCTCC AB 2016181T).
[Mh] Termos MeSH primário: Hibiscus/microbiologia
Paracoccus/classificação
Filogenia
Rizosfera
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Paracoccus/genética
Paracoccus/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001874


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[PMID]:28282039
[Au] Autor:Toyofuku M; Morinaga K; Hashimoto Y; Uhl J; Shimamura H; Inaba H; Schmitt-Kopplin P; Eberl L; Nomura N
[Ad] Endereço:Department of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
[Ti] Título:Membrane vesicle-mediated bacterial communication.
[So] Source:ISME J;11(6):1504-1509, 2017 Jun.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The classical quorum-sensing (QS) model is based on the assumption that diffusible signaling molecules accumulate in the culture medium until they reach a critical concentration upon which expression of target genes is triggered. Here we demonstrate that the hydrophobic signal N-hexadecanoyl-L-homoserine lactone, which is produced by Paracoccus sp., is released from cells by the aid of membrane vesicles (MVs). Packed into MVs, the signal is not only solubilized in an aqueous environment but is also delivered with varying propensities to different bacteria. We propose a novel MV-based mechanism for binary trafficking of hydrophobic signal molecules, which may be particularly relevant for bacteria that live in open aqueous environments.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Paracoccus/fisiologia
Vesículas Transportadoras/fisiologia
[Mh] Termos MeSH secundário: 4-Butirolactona/metabolismo
Membrana Celular/fisiologia
Percepção de Quorum
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2017.13


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[PMID]:28130297
[Au] Autor:Chen C; Shen Y; An D; Voordouw G
[Ad] Endereço:Petroleum Microbiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada cchen@hit.edu.cn.
[Ti] Título:Use of Acetate, Propionate, and Butyrate for Reduction of Nitrate and Sulfate and Methanogenesis in Microcosms and Bioreactors Simulating an Oil Reservoir.
[So] Source:Appl Environ Microbiol;83(7), 2017 Apr 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acetate, propionate, and butyrate (volatile fatty acids [VFA]) occur in oil field waters and are frequently used for microbial growth of oil field consortia. We determined the kinetics of use of these VFA components (3 mM each) by an anaerobic oil field consortium in microcosms containing 2 mM sulfate and 0, 4, 6, 8, or 13 mM nitrate. Nitrate was reduced first, with a preference for acetate and propionate. Sulfate reduction then proceeded with propionate (but not butyrate) as the electron donor, whereas the fermentation of butyrate (but not propionate) was associated with methanogenesis. Microbial community analyses indicated that and ( - ), , and - were the dominant taxa whose members catalyzed these three processes. Most-probable-number assays showed the presence of up to 10 /ml of propionate-oxidizing sulfate-reducing bacteria (SRB) in waters from the Medicine Hat Glauconitic C field. Bioreactors with the same concentrations of sulfate and VFA responded similarly to increasing concentrations of injected nitrate as observed in the microcosms: sulfide formation was prevented by adding approximately 80% of the nitrate dose needed to completely oxidize VFA to CO in both. Thus, this work has demonstrated that simple time-dependent observations of the use of acetate, propionate, and butyrate for nitrate reduction, sulfate reduction, and methanogenesis in microcosms are a good proxy for these processes in bioreactors, monitoring of which is more complex. Oil field volatile fatty acids acetate, propionate, and butyrate were specifically used for nitrate reduction, sulfate reduction, and methanogenic fermentation. Time-dependent analyses of microcosms served as a good proxy for these processes in a bioreactor, mimicking a sulfide-producing (souring) oil reservoir: 80% of the nitrate dose required to oxidize volatile fatty acids to CO was needed to prevent souring in both. Our data also suggest that propionate is a good substrate to enumerate oil field SRB.
[Mh] Termos MeSH primário: Reatores Biológicos
Ácidos Graxos Voláteis/metabolismo
Metano/biossíntese
Consórcios Microbianos
Nitratos/metabolismo
Campos de Petróleo e Gás
Sulfatos/metabolismo
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Bactérias/classificação
Bactérias/efeitos dos fármacos
Bactérias/isolamento & purificação
Bactérias/metabolismo
Biodegradação Ambiental
Ácido Butírico/metabolismo
Simulação por Computador
Metano/metabolismo
Nitratos/farmacologia
Oxirredução
Paracoccus/isolamento & purificação
Paracoccus/metabolismo
Propionatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Fatty Acids, Volatile); 0 (Nitrates); 0 (Propionates); 0 (Sulfates); 107-92-6 (Butyric Acid); OP0UW79H66 (Methane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE


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[PMID]:28065749
[Au] Autor:Maruthiah T; Palavesam A
[Ad] Endereço:Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam-629 502, Kanyakumari District, Tamilnadu, India.
[Ti] Título:Characterization of haloalkalophilic organic solvent tolerant protease for chitin extraction from shrimp shell waste.
[So] Source:Int J Biol Macromol;97:552-560, 2017 Apr.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Halophilic organic solvent tolerant protease (HOSP) producing Paracoccus saliphilus APCMST-CS5 was isolated from the marine sediment samples and identified through 16S rRNA sequence analysis. P. saliphilus APCMST-CS5 registered maximum HOSP production of 1,321.70U/ml in the medium contained the most significant parameters such as shrimp shell powder (SSP), CaCl , NaCl, and sardinella powder (SP), obtained through Placket-Burman and Response Surface Methods. HOSP was further purified to 22.68 fold purity with 29.71 U/mg specific activity and its molecular weight was 39kDa. The HOSP was stable at 60°C, 9.0 pH, 3.0M NaCl concentration and it also showed maximum activity at other tested parameters. Interestingly the purified HOSP showed better antibiofilm ability against tested pathogens. Also, the HOSP effectively deproteinized (85.64%) shrimp shell chitin which in turn maximum and exhibited higher antioxidant activity. The commercial and experimental shrimp shell chitin showed similar peak pattern in FTIR and C CP/MAS NMR spectral analysis.
[Mh] Termos MeSH primário: Exoesqueleto/química
Quitina/isolamento & purificação
Decápodes (Crustáceos)/química
Compostos Orgânicos/farmacologia
Peptídeo Hidrolases/metabolismo
Solventes/farmacologia
Resíduos
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Antioxidantes/isolamento & purificação
Biofilmes/efeitos dos fármacos
Compostos de Bifenilo/química
Quitina/química
Estabilidade Enzimática/efeitos dos fármacos
Sedimentos Geológicos/microbiologia
Peso Molecular
Paracoccus/enzimologia
Paracoccus/genética
Paracoccus/isolamento & purificação
Peptídeo Hidrolases/química
Peptídeo Hidrolases/farmacologia
Picratos/química
Proteólise/efeitos dos fármacos
RNA Ribossômico 16S/genética
Sais/farmacologia
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Organic Chemicals); 0 (Picrates); 0 (RNA, Ribosomal, 16S); 0 (Salts); 0 (Solvents); 0 (Waste Products); 1398-61-4 (Chitin); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170110
[St] Status:MEDLINE


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[PMID]:27856234
[Au] Autor:Kudoh K; Kubota G; Fujii R; Kawano Y; Ihara M
[Ad] Endereço:Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Nagano 399-4511, Japan.
[Ti] Título:Exploration of the 1-deoxy-d-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system.
[So] Source:J Biosci Bioeng;123(3):300-307, 2017 Mar.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:1-Deoxy-d-xylulose 5-phosphate synthase (DXS) is a rate-limiting enzyme in the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway, which is responsible for production of two precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate (DMAPP). Previously, we attempted the overexpression of endogenous DXS in Synechocystis sp. PCC6803, and revealed that although the mRNA level was 4-fold higher, the DXS protein level was only 1.5-fold higher compared with those of the original strain, suggesting the lability of endogenous DXS protein. Therefore, for the creation of a robust isoprenoid synthesis system, it is necessary to build a novel MEP pathway by combining stable enzymes. In this study, we expressed 11 dxs genes from 9 organisms in Escherichia coli and analyzed their protein solubility. Furthermore, we purified the recombinant DXSes and evaluated their specific activities and protease tolerance, thermostability, and feedback inhibition tolerance. Among DXSes we examined in this study, the highest protein solubility was observed in Paracoccus aminophilus DXS (PaDXS). The DXS with the highest activity was one from Rhodobacter capsulatus (RcDXSA). The highest protease tolerance, thermostability, and tolerance of feedback inhibition were found in Bacillus subtilis DXS (BsDXS), RcDXSA, PaDXS, BsDXS, respectively. These DXSes can be potentially used for the design of robust isoprenoid synthesis system.
[Mh] Termos MeSH primário: Escherichia coli/genética
Escherichia coli/metabolismo
Terpenos/metabolismo
Transferases/genética
Transferases/metabolismo
[Mh] Termos MeSH secundário: Bacillus subtilis/enzimologia
Bacillus subtilis/genética
Estabilidade Enzimática
Eritritol/análogos & derivados
Eritritol/biossíntese
Hemiterpenos/biossíntese
Hemiterpenos/metabolismo
Compostos Organofosforados/metabolismo
Paracoccus/enzimologia
Paracoccus/genética
Pentosefosfatos/biossíntese
Peptídeo Hidrolases/metabolismo
Rhodobacter capsulatus/enzimologia
Rhodobacter capsulatus/genética
Solubilidade
Fosfatos Açúcares/biossíntese
Synechocystis/genética
Synechocystis/metabolismo
Transferases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1-deoxylulose 5-phosphate); 0 (2-C-methylerythritol 4-phosphate); 0 (Hemiterpenes); 0 (Organophosphorus Compounds); 0 (Pentosephosphates); 0 (Sugar Phosphates); 0 (Terpenes); 358-71-4 (isopentenyl pyrophosphate); 358-72-5 (3,3-dimethylallyl pyrophosphate); EC 2.- (Transferases); EC 2.2.1.- (deoxyxylulose-5-phosphate synthase); EC 3.4.- (Peptide Hydrolases); RA96B954X6 (Erythritol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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[PMID]:27912769
[Au] Autor:van Zyl LJ; Nemavhulani S; Cass J; Cowan DA; Trindade M
[Ad] Endereço:Institute for Microbial Biotechnology and Metagenomics (IMBM), Department of Biotechnology, University of the Western Cape, Robert Sobukwe Road, Bellville, Cape Town, 7535, South Africa. vanzyllj@gmail.com.
[Ti] Título:Three novel bacteriophages isolated from the East African Rift Valley soda lakes.
[So] Source:Virol J;13(1):204, 2016 Dec 03.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Soda lakes are unique environments in terms of their physical characteristics and the biology they harbour. Although well studied with respect to their microbial composition, their viral compositions have not, and consequently few bacteriophages that infect bacteria from haloalkaline environments have been described. METHODS: Bacteria were isolated from sediment samples of lakes Magadi and Shala. Three phages were isolated on two different Bacillus species and one Paracoccus species using agar overlays. The growth characteristics of each phage in its host was investigated and the genome sequences determined and analysed by comparison with known phages. RESULTS: Phage Shbh1 belongs to the family Myoviridae while Mgbh1 and Shpa belong to the Siphoviridae family. Tetranucleotide usage frequencies and G + C content suggests that Shbh1 and Mgbh1 do not regularly infect, and have therefore not evolved with, the hosts they were isolated on here. Shbh1 was shown capable of infecting two different Bacillus species from the two different lakes demonstrating its potential broad-host range. Comparative analysis of their genome sequence with known phages revealed that, although novel, Shbh1 does share substantial amino acid similarity with previously described Bacillus infecting phages (Grass, phiNIT1 and phiAGATE) and belongs to the Bastille group, while Mgbh1 and Shpa are highly novel. CONCLUSION: The addition of these phages to current databases should help with metagenome/metavirome annotation efforts. We describe a highly novel Paracoccus infecting virus (Shpa) which together with NgoΦ6 and vB_PmaS_IMEP1 is one of only three phages known to infect Paracoccus species but does not show similarity to these phages.
[Mh] Termos MeSH primário: Bacillus/virologia
Bacteriófagos/classificação
Bacteriófagos/isolamento & purificação
Lagos/virologia
Paracoccus/virologia
[Mh] Termos MeSH secundário: África Oriental
Bacillus/isolamento & purificação
Bacteriófagos/genética
Bacteriófagos/crescimento & desenvolvimento
Composição de Bases
DNA Viral/química
DNA Viral/genética
Genoma Viral
Especificidade de Hospedeiro
Lagos/microbiologia
Myoviridae/classificação
Myoviridae/genética
Myoviridae/crescimento & desenvolvimento
Myoviridae/isolamento & purificação
Paracoccus/isolamento & purificação
Análise de Sequência de DNA
Siphoviridae/classificação
Siphoviridae/genética
Siphoviridae/crescimento & desenvolvimento
Siphoviridae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE


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[PMID]:27755578
[Au] Autor:Mulla SI; Sun Q; Hu A; Wang Y; Ashfaq M; Eqani SA; Yu CP
[Ad] Endereço:CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
[Ti] Título:Evaluation of Sulfadiazine Degradation in Three Newly Isolated Pure Bacterial Cultures.
[So] Source:PLoS One;11(10):e0165013, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study is aimed to assess the biodegradation of sulfadiazine (SDZ) and characterization of heavy metal resistance in three pure bacterial cultures and also their chemotactic response towards 2-aminopyrimidine. The bacterial cultures were isolated from pig manure, activated sludge and sediment samples, by enrichment technique on SDZ (6 mg L-1). Based on the 16S rRNA gene sequence analysis, the microorganisms were identified within the genera of Paracoccus, Methylobacterium and Kribbella, which were further designated as SDZ-PM2-BSH30, SDZ-W2-SJ40 and SDZ-3S-SCL47. The three identified pure bacterial strains degraded up to 50.0, 55.2 and 60.0% of SDZ (5 mg L-1), respectively within 290 h. On the basis of quadrupole time-of-flight mass spectrometry and high performance liquid chromatography, 2-aminopyrimidine and 4-hydroxy-2-aminopyrimidine were identified as the main intermediates of SDZ biodegradation. These bacteria were also able to degrade the metabolite, 2-aminopyrimidine, of the SDZ. Furthermore, SDZ-PM2-BSH30, SDZ-W2-SJ40 and SDZ-3S-SCL47 also showed resistance to various heavy metals like copper, cadmium, chromium, cobalt, lead, nickel and zinc. Additionally, all three bacteria exhibited positive chemotaxis towards 2-aminopyrimidine based on the drop plate method and capillary assay. The results of this study advanced our understanding about the microbial degradation of SDZ, which would be useful towards the future SDZ removal in the environment.
[Mh] Termos MeSH primário: Anti-Infecciosos/metabolismo
Bactérias/metabolismo
Sulfadiazina/metabolismo
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Actinobacteria/efeitos dos fármacos
Actinobacteria/genética
Actinobacteria/isolamento & purificação
Animais
Anti-Infecciosos/análise
Anti-Infecciosos/farmacologia
Bactérias/efeitos dos fármacos
Bactérias/genética
Bactérias/isolamento & purificação
Quimiotaxia
Cromatografia Líquida de Alta Pressão
Farmacorresistência Bacteriana
Sedimentos Geológicos/microbiologia
Esterco/microbiologia
Espectrometria de Massas
Metais Pesados/toxicidade
Methylobacterium/classificação
Methylobacterium/efeitos dos fármacos
Methylobacterium/genética
Methylobacterium/isolamento & purificação
Testes de Sensibilidade Microbiana
Paracoccus/classificação
Paracoccus/efeitos dos fármacos
Paracoccus/genética
Paracoccus/isolamento & purificação
Filogenia
Pirimidinas/análise
Pirimidinas/isolamento & purificação
RNA Ribossômico 16S/química
RNA Ribossômico 16S/metabolismo
Análise de Sequência de DNA
Esgotos/microbiologia
Sulfadiazina/análise
Sulfadiazina/farmacologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Manure); 0 (Metals, Heavy); 0 (Pyrimidines); 0 (RNA, Ribosomal, 16S); 0 (Sewage); 0N7609K889 (Sulfadiazine); 109-12-6 (2-aminopyrimidine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0165013



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