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Pesquisa : B03.440.400.425.622 [Categoria DeCS]
Referências encontradas : 524 [refinar]
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[PMID]:29198862
[Au] Autor:Sall C; Ayé M; Bottzeck O; Praud A; Blache Y
[Ad] Endereço:Laboratoire de chimie, UFR des Sciences de la Santé, Université de Thiès, BP 967 Thiès, Senegal.
[Ti] Título:Towards smart biocide-free anti-biofilm strategies: Click-based synthesis of cinnamide analogues as anti-biofilm compounds against marine bacteria.
[So] Source:Bioorg Med Chem Lett;28(2):155-159, 2018 01 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A set of triazole-based analogues of N-coumaroyltyramine was designed to discover potential leads that may help in the control of bacterial biofilms. the most potent compounds act as inhibitors of biofilm development with EC50 closed to ampicillin (EC50 = 11 µM) without toxic effect on bacterial growth even at high concentrations(100 µM).
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Ácidos Cumáricos/farmacologia
Paracoccus/efeitos dos fármacos
Pseudoalteromonas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/síntese química
Antibacterianos/química
Ácidos Cumáricos/síntese química
Ácidos Cumáricos/química
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Estrutura Molecular
Paracoccus/crescimento & desenvolvimento
Pseudoalteromonas/crescimento & desenvolvimento
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Coumaric Acids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


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[PMID]:28892091
[Au] Autor:de Rond T; Stow P; Eigl I; Johnson RE; Chan LJG; Goyal G; Baidoo EEK; Hillson NJ; Petzold CJ; Sarpong R; Keasling JD
[Ad] Endereço:Department of Chemistry, University of California, Berkeley, California, USA.
[Ti] Título:Oxidative cyclization of prodigiosin by an alkylglycerol monooxygenase-like enzyme.
[So] Source:Nat Chem Biol;13(11):1155-1157, 2017 Nov.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prodiginines, which are tripyrrole alkaloids displaying a wide array of bioactivities, occur as linear and cyclic congeners. Identification of an unclustered biosynthetic gene led to the discovery of the enzyme responsible for catalyzing the regiospecific C-H activation and cyclization of prodigiosin to cycloprodigiosin in Pseudoalteromonas rubra. This enzyme is related to alkylglycerol monooxygenase and unrelated to RedG, the Rieske oxygenase that produces cyclized prodiginines in Streptomyces, implying convergent evolution.
[Mh] Termos MeSH primário: Oxigenases de Função Mista/metabolismo
Prodigiosina/metabolismo
Pseudoalteromonas/enzimologia
[Mh] Termos MeSH secundário: Catálise
Ciclização
Evolução Molecular
Indóis/metabolismo
Oxirredução
Prodigiosina/análogos & derivados
Pseudoalteromonas/genética
Pirróis/metabolismo
Streptomyces/enzimologia
Streptomyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Pyrroles); 24311-00-0 (prodiginine); 86797-91-3 (cycloprodigiosin); EC 1.- (Mixed Function Oxygenases); EC 1.14.16.5 (glyceryl-ether monooxygenase); OL369FU7CJ (Prodigiosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2471


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[PMID]:28792373
[Au] Autor:Beurmann S; Ushijima B; Svoboda CM; Videau P; Smith AM; Donachie SP; Aeby GS; Callahan SM
[Ad] Endereço:†â€‹Present address: Institute for GenomeSciences, School of Medicine, University of Maryland, Baltimore, MD 21201, USA. 2​Hawai'i Institute of Marine Biology, 46-007 Lilipuna Road, Kane'ohe, HI 96744, USA 1​Department of Microbiology, University of Hawai'i at Manoa, 2538 McCarthy Mall, Snyder H
[Ti] Título:Pseudoalteromonas piratica sp. nov., a budding, prosthecate bacterium from diseased Montipora capitata, and emended description of the genus Pseudoalteromonas.
[So] Source:Int J Syst Evol Microbiol;67(8):2683-2688, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, motile, rod-shaped bacterium designated OCN003T was cultivated from mucus taken from a diseased colony of the coral Montipora capitata in Kane'ohe Bay, O'ahu, Hawai'i. Colonies of OCN003T were pale yellow, 1-3 mm in diameter, convex, smooth and entire. The strain was heterotrophic, strictly aerobic and strictly halophilic. Cells of OCN003T produced buds on peritrichous prosthecae. Growth occurred within the pH range of 5.5 to 10, and the temperature range of 14 to 39 °C. Major fatty acids were 16 : 1ω7c, 16 : 0, 18 : 1ω7c, 17 : 1ω8c, 12 : 0 3-OH and 17 : 0. Phylogenetic analysis of 1399 nucleotides of the 16S rRNA gene nucleotide sequence and a multi-locus sequence analysis of three genes placed OCN003T in the genus Pseudoalteromonas and indicated that the nearest relatives described are Pseudoalteromonas spongiae, P. luteoviolacea, P. ruthenica and P. phenolica(97-99 % sequence identity). The DNA G+C content of the strain's genome was 40.0 mol%. Based on in silico DNA-DNA hybridization and phenotypic differences from related type strains, we propose that OCN003T represents the type strain of a novel species in the genus Pseudoalteromonas, proposed as Pseudoalteromonas piratica sp. nov. OCN003T (=CCOS1042T=CIP 111189T). An emended description of the genus Pseudoalteromonas is presented.
[Mh] Termos MeSH primário: Antozoários/microbiologia
Filogenia
Pseudoalteromonas/classificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hawaii
Processos Heterotróficos
Hibridização de Ácido Nucleico
Pigmentação
Pseudoalteromonas/genética
Pseudoalteromonas/isolamento & purificação
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001995


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[PMID]:28640915
[Au] Autor:Wu YH; Cheng H; Xu L; Jin XB; Wang CS; Xu XW
[Ad] Endereço:Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, P. R. China.
[Ti] Título:Physiological and genomic features of a novel violacein-producing bacterium isolated from surface seawater.
[So] Source:PLoS One;12(6):e0179997, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Strains JW1T and JW3, isolated from surface seawater of the Arabian Sea, were subjected to polyphasic taxonomic analysis. Cells of both strains were Gram-stain-negative, aerobic, and rod-shaped. They formed violet pigment and produced violacein. On the basis of 16S rRNA gene sequence analysis, strains JW1T and JW3 showed high 16S rRNA gene sequence similarity with Pseudoalteromonas byunsanensis JCM12483T (98.2%), P. shioyasakiensis SE3T (97.8%), P. arabiensis JCM 17292T (97.3%), and P. gelatinilytica NH153T (97.1%). The 16S rRNA gene sequence similarity between JW1T and JW3 was 100%. Phylogenetic analyses revealed that both strains fell within the cluster of the genus Pseudoalteromonas and represented an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between JW1T and type strains of the closely related Pseudoalteromonas species were 70.9-83.3% and 20.0-26.4%, respectively. The sole respiratory quinone in both strains is ubiquinone 8 (Q-8). The principal fatty acids are summed feature 3 (C16:1ω7c and/or iso-C15:0 2OH), C18:1ω7c, and C16:0. The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, one unidentified glycolipid, one unidentified aminolipid, and one unidentified phospholipid. The DNA G+C content was 43.3 mol%. Differential phylogenetic distinctiveness, chemotaxonomic differences, and phenotypic properties indicated that strains JW1T and JW3 could be differentiated from the Pseudoalteromonas species with validly published names. Therefore, it is proposed that strains JW1T and JW3 represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas amylolytica sp. nov. (type strain, JW1T = CGMCC 1.15681T = KCTC 52406T = MCCC 1K02162T) is proposed.
[Mh] Termos MeSH primário: Genômica
Indóis/metabolismo
Pseudoalteromonas/genética
Pseudoalteromonas/fisiologia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Simulação por Computador
DNA Bacteriano/genética
Fenótipo
Filogenia
Pseudoalteromonas/classificação
Pseudoalteromonas/metabolismo
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Indoles); 0 (RNA, Ribosomal, 16S); QJH0DSQ3SG (violacein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179997


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[PMID]:28298441
[Au] Autor:Ito T; Murai M; Ninokura S; Kitazumi Y; Mezic KG; Cress BF; Koffas MAG; Morgan JE; Barquera B; Miyoshi H
[Ad] Endereço:From the Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan and.
[Ti] Título:Identification of the binding sites for ubiquinone and inhibitors in the Na -pumping NADH-ubiquinone oxidoreductase from by photoaffinity labeling.
[So] Source:J Biol Chem;292(19):7727-7742, 2017 May 12.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Na -pumping NADH-quinone oxidoreductase (Na -NQR) is the first enzyme of the respiratory chain and the main ion transporter in many marine and pathogenic bacteria, including The Na -NQR has been extensively studied, but its binding sites for ubiquinone and inhibitors remain controversial. Here, using a photoreactive ubiquinone PUQ-3 as well as two aurachin-type inhibitors [ I]PAD-1 and [ I]PAD-2 and photoaffinity labeling experiments on the isolated enzyme, we demonstrate that the ubiquinone ring binds to the NqrA subunit in the regions Leu-32-Met-39 and Phe-131-Lys-138, encompassing the rear wall of a predicted ubiquinone-binding cavity. The quinolone ring and alkyl side chain of aurachin bound to the NqrB subunit in the regions Arg-43-Lys-54 and Trp-23-Gly-89, respectively. These results indicate that the binding sites for ubiquinone and aurachin-type inhibitors are in close proximity but do not overlap one another. Unexpectedly, although the inhibitory effects of PAD-1 and PAD-2 were almost completely abolished by certain mutations in NqrB ( G140A and E144C), the binding reactivities of [ I]PAD-1 and [ I]PAD-2 to the mutated enzymes were unchanged compared with those of the wild-type enzyme. We also found that photoaffinity labeling by [ I]PAD-1 and [ I]PAD-2, rather than being competitively suppressed in the presence of other inhibitors, is enhanced under some experimental conditions. To explain these apparently paradoxical results, we propose models for the catalytic reaction of Na -NQR and its interactions with inhibitors on the basis of the biochemical and biophysical results reported here and in previous work.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Complexo I de Transporte de Elétrons/química
Quinona Redutases/química
Ubiquinona/química
Vibrio cholerae/enzimologia
[Mh] Termos MeSH secundário: Sítios de Ligação
Catálise
Simulação por Computador
Cristalografia por Raios X
Transporte de Elétrons
Inibidores Enzimáticos/química
Ácidos Graxos Insaturados/química
Lactonas/química
Espectrometria de Massas
Estrutura Molecular
Mutação
Marcadores de Fotoafinidade
Ligação Proteica
Pseudoalteromonas/química
Quinolonas/química
Sódio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Enzyme Inhibitors); 0 (Fatty Acids, Unsaturated); 0 (Lactones); 0 (Photoaffinity Labels); 0 (Quinolones); 0 (korormicin); 108354-13-8 (aurachin D); 1339-63-5 (Ubiquinone); 9NEZ333N27 (Sodium); EC 1.6.5.3 (Electron Transport Complex I); EC 1.6.99.- (Quinone Reductases); EC 1.6.99.5 (NADH dehydrogenase (quinone))
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.781393


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[PMID]:28290654
[Au] Autor:Ulaganathan T; Boniecki MT; Foran E; Buravenkov V; Mizrachi N; Banin E; Helbert W; Cygler M
[Ad] Endereço:Department of Biochemistry, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5E5, Canada.
[Ti] Título:New Ulvan-Degrading Polysaccharide Lyase Family: Structure and Catalytic Mechanism Suggests Convergent Evolution of Active Site Architecture.
[So] Source:ACS Chem Biol;12(5):1269-1280, 2017 May 19.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ulvan is a complex sulfated polysaccharide biosynthesized by green seaweed and contains predominantly rhamnose, xylose, and uronic acid sugars. Ulvan-degrading enzymes have only recently been identified and added to the CAZy ( www.cazy.org ) database as family PL24, but neither their structure nor catalytic mechanism(s) are yet known. Several homologous, new ulvan lyases, have been discovered in Pseudoalteromonas sp. strain PLSV, Alteromonas LOR, and Nonlabens ulvanivorans, defining a new family PL25, with the lyase encoded by the gene PLSV_3936 being one of them. This enzyme cleaves the glycosidic bond between 3-sulfated rhamnose (R3S) and glucuronic acid (GlcA) or iduronic acid (IdoA) via a ß-elimination mechanism. We report the crystal structure of PLSV_3936 and its complex with a tetrasaccharide substrate. PLSV_3936 folds into a seven-bladed ß-propeller, with each blade consisting of four antiparallel ß-strands. Sequence conservation analysis identified a highly conserved region lining at one end of a deep crevice on the protein surface. The putative active site was identified by mutagenesis and activity measurements. Crystal structure of the enzyme with a bound tetrasaccharide substrate confirmed the identity of base and acid residues and allowed determination of the catalytic mechanism and also the identification of residues neutralizing the uronic acid carboxylic group. The PLSV_3936 structure provides an example of a convergent evolution among polysaccharide lyases toward a common active site architecture embedded in distinct folds.
[Mh] Termos MeSH primário: Domínio Catalítico
Evolução Molecular
Polissacarídeo-Liase/química
[Mh] Termos MeSH secundário: Biocatálise
Domínio Catalítico/genética
Sequência Conservada
Cristalografia por Raios X
Estrutura Molecular
Polissacarídeos
Pseudoalteromonas/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides); 0 (ulvan); EC 4.2.2.- (Polysaccharide-Lyases); EC 4.2.2.- (ulvan-lyase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00126


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[PMID]:28280714
[Au] Autor:Casillo A; Papa R; Ricciardelli A; Sannino F; Ziaco M; Tilotta M; Selan L; Marino G; Corsaro MM; Tutino ML; Artini M; Parrilli E
[Ad] Endereço:Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo Naples, Italy.
[Ti] Título:Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic TAC125 against Biofilm.
[So] Source:Front Cell Infect Microbiol;7:46, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is a harmless human skin colonizer responsible for ~20% of orthopedic device-related infections due to its capability to form biofilm. Nowadays there is an interest in the development of anti-biofilm molecules. Marine bacteria represent a still underexploited source of biodiversity able to synthesize a broad range of bioactive compounds, including anti-biofilm molecules. Previous results have demonstrated that the culture supernatant of Antarctic marine bacterium TAC125 impairs the formation of biofilm. Further, evidence supports the hydrophobic nature of the active molecule, which has been suggested to act as a signal molecule. In this paper we describe an efficient activity-guided purification protocol which allowed us to purify this anti-biofilm molecule and structurally characterize it by NMR and mass spectrometry analyses. Our results demonstrate that the anti-biofilm molecule is pentadecanal, a long-chain fatty aldehyde, whose anti- biofilm activity has been assessed using both static and dynamic biofilm assays. The specificity of its action on biofilm has been demonstrated by testing chemical analogs of pentadecanal differing either in the length of the aliphatic chain or in their functional group properties. Further, indications of the mode of action of pentadecanal have been collected by studying the bioluminescence of a reporter strain for the detection of autoinducer AI-2 like activities. The data collected suggest that pentadecanal acts as an AI-2 signal. Moreover, the aldehyde metabolic role and synthesis in the Antarctic source strain has been investigated. To the best of our knowledge, this is the first report on the identification of an anti-biofilm molecule form from cold-adapted bacteria and on the action of a long-chain fatty aldehyde acting as an anti-biofilm molecule against .
[Mh] Termos MeSH primário: Aldeídos/farmacologia
Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Pseudoalteromonas/metabolismo
Staphylococcus epidermidis/efeitos dos fármacos
Staphylococcus epidermidis/fisiologia
[Mh] Termos MeSH secundário: Aldeídos/química
Aldeídos/isolamento & purificação
Regiões Antárticas
Antibacterianos/química
Antibacterianos/isolamento & purificação
Homosserina/análogos & derivados
Homosserina/química
Homosserina/isolamento & purificação
Homosserina/farmacologia
Lactonas/química
Lactonas/isolamento & purificação
Lactonas/farmacologia
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
Pseudoalteromonas/isolamento & purificação
Vibrio/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (Anti-Bacterial Agents); 0 (Lactones); 0 (N-octanoylhomoserine lactone); 2765-11-9 (pentadecanal); 6KA95X0IVO (Homoserine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00046


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[PMID]:28083715
[Au] Autor:Dang HT; Komatsu S; Masuda H; Enomoto K
[Ad] Endereço:School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami, Kochi, 782-8502, Japan.
[Ti] Título:Characterization of LuxI and LuxR Protein Homologs of N-Acylhomoserine Lactone-Dependent Quorum Sensing System in Pseudoalteromonas sp. 520P1.
[So] Source:Mar Biotechnol (NY);19(1):1-10, 2017 Feb.
[Is] ISSN:1436-2236
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudoalteromonas sp. 520P1 (hereafter referred to as strain 520P1) produces N-acylhomoserine lactones (AHLs), which serve as signaling molecules in Gram-negative bacterial quorum sensing. In a previous genomic analysis of the 5.25-Mb genome of strain 520P1, we detected the presence of at least one homolog of the AHL synthase gene (luxI) and five homologs of the transcriptional regulator protein gene (luxR). The LuxI homolog of strain 520P1 (PalI) contained the conserved amino acid motifs shared by all the LuxI family proteins of the different species examined here. The palI gene expressed in Escherichia coli produced two types of AHLs. In the thin-layer chromatography analysis, these AHLs showed identical mobility to the AHLs produced by strain 520P1. The five LuxR homologs of strain 520P1 (PalR1-PalR5) shared only 17-34% amino acid sequence identity, although higher identities were observed in the C-terminal DNA-binding domain. Among the five PalRs, only PalR5 displayed close homology with LuxR family proteins from other Pseudoalteromonas strains. Notably, the palR3 and palI genes were located close together and only 1021 bases apart in the genome. No cognate luxI homolog associated with the four other palR genes was detected. These characteristics of PalI and the PalRs suggest that AHL autoinducers generated by the PalI enzyme might regulate cellular metabolism in cooperation with five transcriptional regulator PalRs, each of which is presumed to play a distinctive role in bacterial signaling.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Regulação Bacteriana da Expressão Gênica
Ligases/genética
Pseudoalteromonas/genética
Percepção de Quorum/genética
Proteínas Repressoras/genética
Transativadores/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Acil-Butirolactonas/metabolismo
Sequência de Aminoácidos
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Genoma Bacteriano
Filogenia
Pseudoalteromonas/classificação
Pseudoalteromonas/enzimologia
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl-Butyrolactones); 0 (Bacterial Proteins); 0 (LuxI protein, Bacteria); 0 (Recombinant Proteins); 0 (Repressor Proteins); 0 (Trans-Activators); 0 (Transcription Factors); 115038-68-1 (LuxR autoinducer binding proteins); EC 6.- (Ligases); EC 6.1.- (N-acylhomoserine lactone synthase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.1007/s10126-016-9726-4


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[PMID]:27989956
[Au] Autor:Sannino F; Parrilli E; Apuzzo GA; de Pascale D; Tedesco P; Maida I; Perrin E; Fondi M; Fani R; Marino G; Tutino ML
[Ad] Endereço:Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia, 80126 Napoli, Italy. Electronic address: filomena.sannino2@unina.it.
[Ti] Título:Pseudoalteromonas haloplanktis produces methylamine, a volatile compound active against Burkholderia cepacia complex strains.
[So] Source:N Biotechnol;35:13-18, 2017 Mar 25.
[Is] ISSN:1876-4347
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 has been reported to produce several Volatile Organic Compounds (VOCs), which are able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. However, no specific antibacterial VOCs have been identified to date. The purpose of the present study was to identify specific VOCs that contribute to Bcc inhibition by the Antarctic strain. When grown on defined medium containing D-gluconate and L-glutamate as carbon, nitrogen and energy sources, P. haloplanktis TAC125 is unable to inhibit the growth of Bcc strains. However, single addition of several amino acids to the defined medium restores the P. haloplanktis TAC125 inhibition ability. With the aim of identifying specific volatile compound/s responsible for Bcc inhibition, we set up an apparatus for VOC capture, accumulation, and storage. P. haloplanktis TAC125 was grown in an automatic fermenter which was connected to a cooling system to condense VOCs present in the exhaust air outlet. Upon addition of methionine to the growth medium, the VOC methylamine was produced by P. haloplanktis TAC125. Methylamine was found to inhibit the growth of several Bcc strains in a dose-dependent way. Although it was reported that P. haloplanktis TAC125 produces VOCs endowed with antimicrobial activity, this is the first demonstration that methylamine probably contributes to the anti-Bcc activity of P. haloplanktis TAC125 VOCs.
[Mh] Termos MeSH primário: Complexo Burkholderia cepacia/efeitos dos fármacos
Metilaminas/metabolismo
Metilaminas/farmacologia
Pseudoalteromonas/metabolismo
[Mh] Termos MeSH secundário: Regiões Antárticas
Antibacterianos/metabolismo
Antibacterianos/farmacologia
Reatores Biológicos/microbiologia
Biotecnologia
Complexo Burkholderia cepacia/crescimento & desenvolvimento
Complexo Burkholderia cepacia/patogenicidade
Meios de Cultura/química
Seres Humanos
Testes de Sensibilidade Microbiana
Pseudoalteromonas/crescimento & desenvolvimento
Pseudoalteromonas/isolamento & purificação
Compostos Orgânicos Voláteis/metabolismo
Compostos Orgânicos Voláteis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Culture Media); 0 (Methylamines); 0 (Volatile Organic Compounds); BSF23SJ79E (methylamine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161220
[St] Status:MEDLINE


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[PMID]:27942842
[Au] Autor:Liu Z; Wang M; Meng X; Li Y; Wang D; Jiang Y; Shao H; Zhang Y
[Ad] Endereço:College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
[Ti] Título:Isolation and Genome Sequencing of a Novel Pseudoalteromonas Phage PH1.
[So] Source:Curr Microbiol;74(2):212-218, 2017 Feb.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The family Pseudoalteromonas is highly adaptable to dissimilar ecological habitats and plays an important ecological role in the marine environment. In this study, a new Pseudoalteromonas phage PH1 was isolated from the Yellow Sea. To better understand the bacteriophage, its biological properties, including morphology, host range, growth phenotype, thermal and pH stability, and nucleic acid composition, were investigated in detail. The result showed that the phage PH1 is a Podoviridae-phage with an icosahedral head (60 nm of diameter) and a short tail (26 nm in length). The phage PH1 genome consists of 42,685 bp length double-stranded DNA with a G+C content of 42.24% and is predicted to have 55 open reading frames (ORFs) with an average length of 740 bp nucleotides each. The phage PH1 genome adds a new Podoviridae-phage genome to marine bacteriophage dataset, which will provide useful basic information for further molecular research on interaction mechanisms between bacteriophages and their hosts.
[Mh] Termos MeSH primário: Bacteriófagos/isolamento & purificação
Genoma Viral
Podoviridae/isolamento & purificação
Pseudoalteromonas/virologia
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Bacteriófagos/classificação
Bacteriófagos/genética
Bacteriófagos/ultraestrutura
Composição de Bases
DNA/química
DNA/genética
DNA Viral/química
DNA Viral/genética
Especificidade de Hospedeiro
Concentração de Íons de Hidrogênio
Fases de Leitura Aberta
Podoviridae/classificação
Podoviridae/genética
Podoviridae/ultraestrutura
Água do Mar/virologia
Temperatura Ambiente
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 9007-49-2 (DNA)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1175-9



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