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[PMID]: | 29239716 |
[Au] Autor: | Sudan SK; Pal D; Bisht B; Kumar N; Chaudhry V; Patil P; Sahni G; Mayilraj S; Krishnamurthi S |
[Ad] Endereço: | 1​Division of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Chandigarh, 160 036, India. |
[Ti] Título: | Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India. |
[So] Source: | Int J Syst Evol Microbiol;68(1):402-408, 2018 Jan. | [Is] ISSN: | 1466-5034 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | A bacterial strain, designated ASS-1 , was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were C16 : 1ω7c/ 16 : 1 C16 : 1ω6c, C18 : 1ω7c and C16 : 0. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1 showed high similarity to Pseudomonas guguanensis CC-G9A (98.2 %), Pseudomonas alcaligenes ATCC 14909 (98.2 %), Pseudomonas oleovorans DSM 1045 (98.1 %), Pseudomonas indolxydans IPL-1 (98.1 %) and Pseudomonas toyotomiensis HT-3 (98.0 %). Analysis of its rpoB and rpoD housekeeping genes confirmed its phylogenetic affiliation and showed identities lower than 93 % with respect to the closest relatives. Phylogenetic analysis based on the 16S rRNA, rpoB, rpoD genes and the whole genome assigned it to the genus Pseudomonas. The results of digital DNA-DNA hybridization based on the genome-to-genome distance calculator and average nucleotide identity revealed low genome relatedness to its close phylogenetic neighbours (below the recommended thresholds of 70 and 95 %, respectively, for species delineation). Strain ASS-1 also differed from the related strains by some phenotypic characteristics, i.e. growth at pH 5.0 and 42 °C, starch and casein hydrolysis, and citrate utilization. Therefore, based on data obtained from phenotypic and genotypic analysis, it is evident that strain ASS-1 should be regarded as a novel species within the genus Pseudomonas, for which the name Pseudomonasfluvialis sp. nov. is proposed. The type strain is ASS-1 (=KCTC 52437 =CCM 8778 ). |
[Mh] Termos MeSH primário: |
Filogenia Pseudomonas/classificação Rios/microbiologia Microbiologia da Água
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[Mh] Termos MeSH secundário: |
Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Ácidos Graxos/química Genes Bacterianos Índia Hibridização de Ácido Nucleico Pigmentação Pseudomonas/genética Pseudomonas/isolamento & purificação RNA Ribossômico 16S/genética Análise de Sequência de DNA
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180209 |
[Lr] Data última revisão:
| 180209 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171215 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1099/ijsem.0.002520 |
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