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[PMID]: 29239716 [Au] Autor: Sudan SK; Pal D; Bisht B; Kumar N; Chaudhry V; Patil P; Sahni G; Mayilraj S; Krishnamurthi S [Ad] Endereço: 1​Division of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Chandigarh, 160 036, India. [Ti] Título: Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India. [So] Source: Int J Syst Evol Microbiol;68(1):402-408, 2018 Jan. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A bacterial strain, designated ASS-1 , was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were C16 : 1ω7c/ 16 : 1 C16 : 1ω6c, C18 : 1ω7c and C16 : 0. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1 showed high similarity to Pseudomonas guguanensis CC-G9A (98.2 %), Pseudomonas alcaligenes ATCC 14909 (98.2 %), Pseudomonas oleovorans DSM 1045 (98.1 %), Pseudomonas indolxydans IPL-1 (98.1 %) and Pseudomonas toyotomiensis HT-3 (98.0 %). Analysis of its rpoB and rpoD housekeeping genes confirmed its phylogenetic affiliation and showed identities lower than 93 % with respect to the closest relatives. Phylogenetic analysis based on the 16S rRNA, rpoB, rpoD genes and the whole genome assigned it to the genus Pseudomonas. The results of digital DNA-DNA hybridization based on the genome-to-genome distance calculator and average nucleotide identity revealed low genome relatedness to its close phylogenetic neighbours (below the recommended thresholds of 70 and 95 %, respectively, for species delineation). Strain ASS-1 also differed from the related strains by some phenotypic characteristics, i.e. growth at pH 5.0 and 42 °C, starch and casein hydrolysis, and citrate utilization. Therefore, based on data obtained from phenotypic and genotypic analysis, it is evident that strain ASS-1 should be regarded as a novel species within the genus Pseudomonas, for which the name Pseudomonasfluvialis sp. nov. is proposed. The type strain is ASS-1 (=KCTC 52437 =CCM 8778 ). [Mh] Termos MeSH primário: Filogenia Pseudomonas/classificação Rios/microbiologia Microbiologia da Água [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Ácidos Graxos/química Genes Bacterianos Índia Hibridização de Ácido Nucleico Pigmentação Pseudomonas/genética Pseudomonas/isolamento & purificação RNA Ribossômico 16S/genética Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180209 [Lr] Data última revisão: 180209 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171215 [St] Status: MEDLINE [do] DOI: 10.1099/ijsem.0.002520

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[PMID]: 29320571 [Au] Autor: Gao P; Qin J; Li D; Zhou S [Ad] Endereço: College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China. [Ti] Título: Inhibitory effect and possible mechanism of a Pseudomonas strain QBA5 against gray mold on tomato leaves and fruits caused by Botrytis cinerea. [So] Source: PLoS One;13(1):e0190932, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The fungal pathogen Botrytis cinerea causes gray mold disease on various hosts, which results in serious economic losses. Over the past several decades, many kinds of fungicides have been used to successfully control the disease. Meanwhile, the uses of fungicides lead to environmental pollution as well as a potential threat to the human health by the chemical residues in tomato fruit. Also, the gray mold disease is difficult to control with fungicides. Therefore, exploring alternative measures such as biological controls could be the best choice to control the disease and alleviate damages caused by fungicides. In this study, we isolated and identified a novel Pseudomonas strain termed as QBA5 from healthy tomato plant based on the morphological, biochemical characteristics and molecular detection. The antifungal activity assays revealed that, in the presence of QBA5, conidia germination, germ tube elongation and mycelial growth of B. cinerea were significantly inhibited. Most importantly, QBA5 exerted a significant preventive effectiveness against gray mold on tomato fruits and plants. The possible mechanism of QBA5 involved in the inhibition of B. cinerea was investigated. It revealed that the conidia plasma membrane of B. cinerea was severely damaged by QBA5. Further, four different antifungal compounds in the supernatant of QBA5 were separated by preparative high performance liquid chromatography (PHPLC). Overall, the data indicate that there is a considerable potential for QBA5 to reduce the damage caused by gray mold disease on tomato. [Mh] Termos MeSH primário: Antibiose Botrytis/fisiologia Frutas/microbiologia Lycopersicon esculentum/microbiologia Doenças das Plantas/microbiologia Folhas de Planta/microbiologia Pseudomonas/fisiologia [Mh] Termos MeSH secundário: Antifúngicos/química Antifúngicos/farmacologia Botrytis/efeitos dos fármacos Membrana Celular/efeitos dos fármacos Membrana Celular/fisiologia Meios de Cultura/química Frutas/efeitos dos fármacos Lycopersicon esculentum/efeitos dos fármacos Doenças das Plantas/prevenção & controle Doenças das Plantas/terapia Folhas de Planta/efeitos dos fármacos Pseudomonas/isolamento & purificação Esporos Fúngicos/efeitos dos fármacos Esporos Fúngicos/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antifungal Agents); 0 (Culture Media) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180111 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0190932

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[PMID]: 29032906 [Au] Autor: Jäger T; Alexander J; Kirchen S; Dötsch A; Wieland A; Hiller C; Schwartz T [Ad] Endereço: Karlsruhe Institute of Technology (KIT) - Campus North, Institute of Functional Interfaces (IFG), Department of Bioengineering and Biosystems, P.O. Box 3640, 76021 Karlsruhe, Germany. [Ti] Título: Live-dead discrimination analysis, qPCR assessment for opportunistic pathogens, and population analysis at ozone wastewater treatment plants. [So] Source: Environ Pollut;232:571-579, 2018 Jan. [Is] ISSN: 1873-6424 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: In respect to direct and indirect water reuse, the microbiological quality of treated wastewater is highly important. Conventional wastewater treatment plants are normally not equipped with advanced technologies for the elimination of bacteria. Molecular biology analyses were combined with live-dead discrimination analysis of wastewater population using Propidium monoazide (PMA) to study population shifts during ozonation (1 g ozone/g DOC) at a municipal wastewater treatment plant. Escherichia coli, enterococci, and Pseudomonas aeruginosa were quantified by polymerase chain reaction (qPCR) and the whole wastewater population was analyzed by metagenomic sequencing. The PMA-qPCR experiments showed that the abundances of P. aeruginosa didn't change by ozone treatment, whereas a reduction was observed for E. coli and enterococci. Results comparing conventional cultivation experiments with PMA-qPCR underlined the presence of viable but not culturable cells (VBNC) and their regrowth potential after ozone treatment. Illumina HiSeq sequencing results with and without PMA treatment demonstrated high population similarities in water samples originating from ozone inflow sampling sides. Upon using PMA treatment after ozonation, population shifts became visible and also underlined the importance of PMA treatment for the evaluation of elimination and selection processes during ozonation at WWTPs. Amongst a number of 14 most abundant genera identified in the inflow samples, 9 genera were found to be reduced, whereas 4 genera increased in relative abundance and 1 genus almost remained constant. The strongest increase in relative abundance after ozonation was detected for Oscillatoria spp., Microcoleus spp. and Nitrospira spp. Beside this, a continuous release of Pseudomonas spp. (including P. aeruginosa) to the downstream receiving body was confirmed. Regrowth experiments demonstrated a high prevalence of P. aeruginosa as part of the surviving bacterial population. Summing up, molecular biology analyses in combination with live-dead discrimination are comprehensive methods to evaluate the elimination processes targeting specific species and/or whole microbial populations. [Mh] Termos MeSH primário: Ozônio/química Eliminação de Resíduos Líquidos/métodos Águas Residuais/microbiologia [Mh] Termos MeSH secundário: Azidas Bactérias Monitoramento Ambiental Escherichia coli/genética Reação em Cadeia da Polimerase Propídio/análogos & derivados Pseudomonas Pseudomonas aeruginosa [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Azides); 0 (Waste Water); 0 (propidium monoazide); 36015-30-2 (Propidium); 66H7ZZK23N (Ozone) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180119 [Lr] Data última revisão: 180119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171017 [St] Status: MEDLINE

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[PMID]: 29267321 [Au] Autor: Gharaie S; Vaas LAI; Rosberg AK; Windstam ST; Karlsson ME; Bergstrand KJ; Khalil S; Wohanka W; Alsanius BW [Ad] Endereço: Swedish University of Agricultural Sciences, Department of Biosystems and Technology, Microbial Horticulture Unit, Alnarp, Sweden. [Ti] Título: Light spectrum modifies the utilization pattern of energy sources in Pseudomonas sp. DR 5-09. [So] Source: PLoS One;12(12):e0189862, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Despite the overruling impact of light in the phyllosphere, little is known regarding the influence of light spectra on non-phototrophic bacteria colonizing the leaf surface. We developed an in vitro method to study phenotypic profile responses of bacterial pure cultures to different bands of the visible light spectrum using monochromatic (blue: 460 nm; red: 660 nm) and polychromatic (white: 350-990 nm) LEDs, by modification and optimization of a protocol for the Phenotype MicroArray™ technique (Biolog Inc., CA, USA). The new protocol revealed high reproducibility of substrate utilization under all conditions tested. Challenging the non-phototrophic bacterium Pseudomonas sp. DR 5-09 with white, blue, and red light demonstrated that all light treatments affected the respiratory profile differently, with blue LED having the most decisive impact on substrate utilization by impairing respiration of 140 substrates. The respiratory activity was decreased on 23 and 42 substrates under red and white LEDs, respectively, while utilization of one, 16, and 20 substrates increased in the presence of red, blue, and white LEDs, respectively. Interestingly, on four substrates contrasting utilization patterns were found when the bacterium was exposed to different light spectra. Although non-phototrophic bacteria do not rely directly on light as an energy source, Pseudomonas sp. DR 5-09 changed its respiratory activity on various substrates differently when exposed to different lights. Thus, ability to sense and distinguish between different wavelengths even within the visible light spectrum must exist, and leads to differential regulation of substrate usage. With these results, we hypothesize that different light spectra might be a hitherto neglected key stimulus for changes in microbial lifestyle and habits of substrate usage by non-phototrophic phyllospheric microbiota, and thus might essentially stratify leaf microbiota composition and diversity. [Mh] Termos MeSH primário: Luz Pseudomonas/efeitos da radiação [Mh] Termos MeSH secundário: Biomassa Folhas de Planta/microbiologia Pseudomonas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180116 [Lr] Data última revisão: 180116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171222 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0189862

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[PMID]: 29289293 [Au] Autor: Ma Y; Zhao Y; Wang Y; Li X; Sun F; Corvini PF; Ji R [Ad] Endereço: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. [Ti] Título: Effects of Cu and humic acids on degradation and fate of TBBPA in pure culture of Pseudomonas sp. strain CDT. [So] Source: J Environ Sci (China);62:60-67, 2017 Dec. [Is] ISSN: 1001-0742 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Soil contamination with tetrabromobisphenol A (TBBPA) has caused great concerns; however, the presence of heavy metals and soil organic matter on the biodegradation of TBBPA is still unclear. We isolated Pseudomonas sp. strain CDT, a TBBPA-degrading bacterium, from activated sludge and incubated it with C-labeled TBBPA for 87 days in the absence and presence of Cu and humic acids (HA). TBBPA was degraded to organic-solvent extractable (59.4%±2.2%) and non-extractable (25.1%±1.3%) metabolites, mineralized to CO (4.8%±0.8%), and assimilated into cells (10.6%±0.9%) at the end of incubation. When Cu was present, the transformation of extractable metabolites into non-extractable metabolites and mineralization were inhibited, possibly due to the toxicity of Cu to cells. HA significantly inhibited both dissipation and mineralization of TBBPA and altered the fate of TBBPA in the culture by formation of HA-bound residues that amounted to 22.1%±3.7% of the transformed TBBPA. The inhibition from HA was attributed to adsorption of TBBPA and formation of bound residues with HA via reaction of reactive metabolites with HA molecules, which decreased bioavailability of TBBPA and metabolites in the culture. When Cu and HA were both present, Cu significantly promoted the HA inhibition on TBBPA dissipation but not on metabolite degradation. The results provide insights into individual and interactive effects of Cu and soil organic matter on the biotransformation of TBBPA and indicate that soil organic matter plays an essential role in determining the fate of organic pollutants in soil and mitigating heavy metal toxicity. [Mh] Termos MeSH primário: Cobre/química Poluentes Ambientais/metabolismo Substâncias Húmicas Bifenil Polibromatos/metabolismo Pseudomonas/fisiologia [Mh] Termos MeSH secundário: Biodegradação Ambiental Cobre/toxicidade Pseudomonas/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Environmental Pollutants); 0 (Humic Substances); 0 (Polybrominated Biphenyls); 789U1901C5 (Copper); FQI02RFC3A (tetrabromobisphenol A) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180103 [Lr] Data última revisão: 180103 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180101 [St] Status: MEDLINE

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[PMID]: 28451809 [Au] Autor: García C; Rendueles M; Díaz M [Ad] Endereço: Department of Chemical and Environmental Engineering, University of Oviedo, C/Julián Clavería s/n, 33071, Oviedo, Spain. [Ti] Título: Microbial amensalism in Lactobacillus casei and Pseudomonas taetrolens mixed culture. [So] Source: Bioprocess Biosyst Eng;40(7):1111-1122, 2017 Jul. [Is] ISSN: 1615-7605 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Pseudomonas taetrolens has recently been revealed as an effective microbial producer of lactobionic acid from carbohydrates contained in dairy byproducts. In terms of food industrial applications, the implementation of lactobionic acid biosynthesis coupled with the classic bacterial production of lactic acid appears an important goal. This research paper studies the simultaneous fermentation of residual cheese whey by P. taetrolens and Lactobacillus casei to co-produce lactic and lactobionic acids. Experimental data showed the importance of the interactions established between the two microorganisms. Changes in physiology, viability, growth, and productive capacity were tested experimentally. Lactobacillus was not seen to suffer any appreciable stress, but considerable variations were observed in the Pseudomonas behavior presumably owing to inhibitory lactic metabolites, interaction that can be classified as microbial amensalism. As to production, lactic acid remained without significant changes in mixed fermentations, whereas the production of lactobionic acid decreased sharply due to the competitive exclusion of Pseudomonas. [Mh] Termos MeSH primário: Lactobacillus casei Pseudomonas [Mh] Termos MeSH secundário: Queijo Fermentação Ácido Láctico Lactobacillus [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 33X04XA5AT (Lactic Acid) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171204 [Lr] Data última revisão: 171204 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170429 [St] Status: MEDLINE [do] DOI: 10.1007/s00449-017-1773-3

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[PMID]: 28938018 [Au] Autor: Cole BJ; Feltcher ME; Waters RJ; Wetmore KM; Mucyn TS; Ryan EM; Wang G; Ul-Hasan S; McDonald M; Yoshikuni Y; Malmstrom RR; Deutschbauer AM; Dangl JL; Visel A [Ad] Endereço: US Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America. [Ti] Título: Genome-wide identification of bacterial plant colonization genes. [So] Source: PLoS Biol;15(9):e2002860, 2017 Sep. [Is] ISSN: 1545-7885 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system. Among the genes we identified were some with obvious colonization-related roles in motility and carbon metabolism, as well as 44 other genes that had no or vague functional predictions. Independent validation assays of individual genes confirmed colonization functions for 20 of 22 (91%) cases tested. To further characterize genes identified by our screen, we compared the functional contributions of P. simiae genes to growth in 90 distinct in vitro conditions by RB-TnSeq, highlighting specific metabolic functions associated with root colonization genes. Our analysis of bacterial genes by sequence-driven saturation mutagenesis revealed a genome-wide map of the genetic determinants of plant root colonization and offers a starting point for targeted improvement of the colonization capabilities of plant-beneficial microbes. [Mh] Termos MeSH primário: Arabidopsis/microbiologia Genes Bacterianos Pseudomonas/genética [Mh] Termos MeSH secundário: Mapeamento Cromossômico Cromossomos Bacterianos Código de Barras de DNA Taxonômico Elementos de DNA Transponíveis DNA Bacteriano Mutação Raízes de Plantas/microbiologia Pseudomonas/crescimento & desenvolvimento [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA Transposable Elements); 0 (DNA, Bacterial) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171022 [Lr] Data última revisão: 171022 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170923 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pbio.2002860

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[PMID]: 28910379 [Au] Autor: Thuy DB; Campbell J; Hoang NVM; Trinh TTT; Duong HTH; Hieu NC; Duy NHA; Hao NV; Baker S; Thwaites GE; Chau NVV; Thwaites CL [Ad] Endereço: Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programmes, Oxford University Clinical Research Unit (OUCRU), Ho Chi Minh City, Vietnam. [Ti] Título: A one-year prospective study of colonization with antimicrobial-resistant organisms on admission to a Vietnamese intensive care unit. [So] Source: PLoS One;12(9):e0184847, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: There is a paucity of data regarding initial bacterial colonization on admission to Intensive Care Units (ICUs) in low and middle-income countries (LMICs). Patients admitted to ICUs in LMICs are at high-risk of subsequent infection with antimicrobial-resistant organisms (AROs). We conducted a prospective, observational study at the Hospital for Tropical Diseases in Ho Chi Minh City, Vietnam from November 2014 to January 2016 to assess the colonization and antimicrobial susceptibility of Staphylococcus aureus, Escherichia coli, Klebsiella spp., Pseudomonas spp. and Acinetobacter spp. among adult patients within 48 hours of ICU admission. We found the admission colonization prevalence (with at least one of the identified organisms) was 93.7% (785/838) and that of AROs was 63.1% (529/838). The colonization frequency with AROs among patients admitted from the community was comparable to those transferred from other hospitals (62.2% vs 63.8%). Staphylococcus aureus was the most commonly isolated bacteria from nasal swabs (13.1%, 110/838) and the methicillin-resistant Staphylococcus aureus nasal colonization prevalence was 8.6% (72/838). We isolated Escherichia coli from rectal swabs from almost all enrolled patients (88.3%, 740/838) and 52.1% (437/838) of patients were colonized by extended spectrum ß-lactamase producing Escherichia coli. Notably, Klebsiella pneumoniae was the most frequently isolated bacteria from the tracheal swabs (11.8%, 18/153). Vietnamese ICU patients have a high rate of colonization with AROs and are thus at risk of subsequent infections with these organisms if good infection control practices are not in place. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Bactérias/efeitos dos fármacos Bactérias/isolamento & purificação Doenças Transmissíveis/epidemiologia Doenças Transmissíveis/microbiologia Farmacorresistência Bacteriana [Mh] Termos MeSH secundário: Acinetobacter/efeitos dos fármacos Acinetobacter/isolamento & purificação Adulto Bactérias/classificação Escherichia coli/efeitos dos fármacos Escherichia coli/isolamento & purificação Feminino Seres Humanos Unidades de Terapia Intensiva Klebsiella/efeitos dos fármacos Klebsiella/isolamento & purificação Masculino Meia-Idade Nariz/microbiologia Prevalência Estudos Prospectivos Pseudomonas/efeitos dos fármacos Pseudomonas/isolamento & purificação Staphylococcus aureus/efeitos dos fármacos Staphylococcus aureus/isolamento & purificação Vietnã/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE; OBSERVATIONAL STUDY [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171012 [Lr] Data última revisão: 171012 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170915 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0184847

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[PMID]: 28880608 [Au] Autor: Wang J; Chen J; Hu Y; Hu H; Liu G; Yan R [Ad] Endereço: 1 Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China. [Ti] Título: Application of a Predictive Growth Model of Pseudomonas spp. for Estimating Shelf Life of Fresh Agaricus bisporus. [So] Source: J Food Prot;80(10):1676-1681, 2017 Oct. [Is] ISSN: 1944-9097 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: For prediction of the shelf life of the mushroom Agaricus bisporus, the growth curve of the main spoilage microorganisms was studied under isothermal conditions at 2 to 22°C with a modified Gompertz model. The effect of temperature on the growth parameters for the main spoilage microorganisms was quantified and modeled using the square root model. Pseudomonas spp. were the main microorganisms causing A. bisporus decay, and the modified Gompertz model was useful for modelling the growth curve of Pseudomonas spp. All the bias factors values of the model were close to 1. By combining the modified Gompertz model with the square root model, a prediction model to estimate the shelf life of A. bisporus as a function of storage temperature was developed. The model was validated for A. bisporus stored at 6, 12, and 18°C, and adequate agreement was found between the experimental and predicted data. [Mh] Termos MeSH primário: Agaricus Microbiologia de Alimentos Pseudomonas/crescimento & desenvolvimento [Mh] Termos MeSH secundário: Temperatura Ambiente Verduras [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171102 [Lr] Data última revisão: 171102 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170908 [St] Status: MEDLINE [do] DOI: 10.4315/0362-028X.JFP-17-055

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[PMID]: 28820109 [Au] Autor: Frasson D; Opoku M; Picozzi T; Torossi T; Balada S; Smits THM; Hilber U [Ad] Endereço: 1​Department of Life Sciences and Facility Management, Zürich University of Applied Sciences ZHAW, Wädenswil, Switzerland 2​Microbiology and Molecular Biology, Institute for Chemistry and Biotechnology (ICBT), Zürich University of Applied Sciences ZHAW, Wädenswil, Switzerland. [Ti] Título: Pseudomonas wadenswilerensis sp. nov. and Pseudomonas reidholzensis sp. nov., two novel species within the Pseudomonas putida group isolated from forest soil. [So] Source: Int J Syst Evol Microbiol;67(8):2853-2861, 2017 Aug. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Within the frame of a biotechnological screening, we isolated two Pseudomonas strains from forest soil. 16S rRNA gene sequence analysis indicated that strain CCOS 864T shared 99.8 % similarity with Pseudomonas donghuensis HYST, while strain CCOS 865T shared 99.0 % similarity with Pseudomonas putida DSM 291T and lower similarity with other P. putida group type strains. Based on multilocus sequence analysis, the two strains were genotypically distinct from each other, each forming a separate clade. Strains CCOS 864T and CCOS 865T were Gram-stain-negative, motile and rod-shaped, growing at a temperature range of 4-37 °C. Strain CCOS 864T could be phenotypically distinguished from P. putida group species by the combination of gelatinase-positive reaction and positive growth on N-acetyl-d-glucosamine, p-hydroxyphenylacetic acid and inosine but lack of fluorescein production on King's B medium, while strain CCOS 865T could be distinguished from P. putida group species by the combination of positive growth with saccharic acid and negative growth with p-hydroxyphenylacetic acid and l-pyroglutamic acid. The major polar lipid for both strains was phosphatidylethanolamine; the major quinone was ubiquinone Q-9. DNA-DNA hybridization and average nucleotide identities confirmed the novel species status for the two strains. The DNA G+C contents of CCOS 864T and CCOS 865T were 62.1 and 63.8 mol%, respectively. The phenotypic, phylogenetic and DNA-DNA relatedness data support the suggestion that CCOS 864T and CCOS 865T represent two novel Pseudomonas species. The names Pseudomonas wadenswilerensis sp. nov. (type strain CCOS 864T=LMG 29327T) and Pseudomonas reidholzensis sp. nov. (type strain CCOS 865T=LMG 29328T) are proposed. [Mh] Termos MeSH primário: Florestas Filogenia Pseudomonas/classificação Microbiologia do Solo [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Ácidos Graxos/química Tipagem de Sequências Multilocus Hibridização de Ácido Nucleico Fosfatidiletanolaminas/química Pseudomonas/genética Pseudomonas/isolamento & purificação Pseudomonas putida RNA Ribossômico 16S/genética Análise de Sequência de DNA Suíça Ubiquinona/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 39382-08-6 (phosphatidylethanolamine); MGW7TYF2DQ (ubiquinone 9) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170919 [Lr] Data última revisão: 170919 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170819 [St] Status: MEDLINE [do] DOI: 10.1099/ijsem.0.002035

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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