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Pesquisa : B03.440.400.425.625.625.350 [Categoria DeCS]
Referências encontradas : 28 [refinar]
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  1 / 28 MEDLINE  
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[PMID]:28187702
[Au] Autor:Chen W; Hu H; Zhang C; Huang F; Zhang D; Zhang H
[Ad] Endereço:Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, 100193, People's Republic of China.
[Ti] Título:Adaptation response of Pseudomonas fragi on refrigerated solid matrix to a moderate electric field.
[So] Source:BMC Microbiol;17(1):32, 2017 Feb 10.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Moderate electric field (MEF) technology is a promising food preservation strategy since it relies on physical properties-rather than chemical additives-to preserve solid cellular foods during storage. However, the effectiveness of long-term MEF exposure on the psychrotrophic microorganisms responsible for the food spoilage at cool temperatures remains unclear. RESULTS: The spoilage-associated psychrotroph Pseudomonas fragi MC16 was obtained from pork samples stored at 7 °C. Continuous MEF treatment attenuated growth and resulted in subsequent adaptation of M16 cultured on nutrient agar plates at 7 °C, compared to the control cultures, as determined by biomass analysis and plating procedures. Moreover, intracellular dehydrogenase activity and ATP levels also indicated an initial effect of MEF treatment followed by cellular recovery, and extracellular ß-galactosidase activity assays indicated no obvious changes in cell membrane permeability. Furthermore, microscopic observations using scanning and transmission electron microscopy revealed that MEF induced sublethal cellular injury during early treatment stages, but no notable changes in morphology or cytology on subsequent days. CONCLUSION: Our study provides direct evidence that psychrotrophic P. fragi MC16 cultured on nutrient agar plates at 7 °C are capable of adapting to MEF treatment.
[Mh] Termos MeSH primário: Eletricidade
Microbiologia de Alimentos
Conservação de Alimentos/métodos
Pseudomonas fragi/crescimento & desenvolvimento
Pseudomonas fragi/metabolismo
Pseudomonas fragi/efeitos da radiação
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/análise
Animais
Biomassa
Permeabilidade da Membrana Celular/fisiologia
Permeabilidade da Membrana Celular/efeitos da radiação
Contagem de Colônia Microbiana
Terapia por Estimulação Elétrica
Ativação Enzimática
Ensaios Enzimáticos
Armazenamento de Alimentos
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Oxirredutases/metabolismo
Oxirredutases/efeitos da radiação
Pseudomonas fragi/enzimologia
Carne Vermelha/microbiologia
Refrigeração
Suínos
Temperatura Ambiente
beta-Galactosidase/metabolismo
beta-Galactosidase/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8L70Q75FXE (Adenosine Triphosphate); EC 1.- (Oxidoreductases); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-017-0945-2


  2 / 28 MEDLINE  
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[PMID]:28040161
[Au] Autor:Wang GY; Wang HH; Han YW; Xing T; Ye KP; Xu XL; Zhou GH
[Ad] Endereço:Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
[Ti] Título:Evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ.
[So] Source:Food Microbiol;63:139-146, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microorganisms play an important role in the spoilage of chilled chicken. In this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. Four isolates, namely Aeromonas salmonicida 35, Pseudomonas fluorescens H5, Pseudomonas fragi H8 and Serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage potential in situ. The in vitro studies showed that A. salmonicida 35 displayed the strongest proteolytic activity against both sarcoplasmic and myofibrillar proteins. However, the major spoilage isolate in situ was P. fragi H8, which exhibited a fast growth rate, slime formation and increased pH and total volatile basic nitrogen (TVBN) on chicken breast fillets. The relative amounts of volatile organic compounds (VOCs) originating from the microorganisms, including alcohols, aldehydes, ketones and several sulfur compounds, increased during storage. In sum, this study demonstrated the characteristics of 4 potential spoilage bacteria on chilled yellow-feather chicken and provides a simple and convenient method to assess spoilage bacteria during quality management.
[Mh] Termos MeSH primário: Aeromonas salmonicida/metabolismo
Galinhas/microbiologia
Aves Domésticas/microbiologia
Pseudomonas/metabolismo
Refrigeração
Serratia liquefaciens/metabolismo
[Mh] Termos MeSH secundário: Aeromonas salmonicida/crescimento & desenvolvimento
Aeromonas salmonicida/isolamento & purificação
Animais
Microbiologia de Alimentos
Armazenamento de Alimentos
Proteólise
Pseudomonas/crescimento & desenvolvimento
Pseudomonas fluorescens/crescimento & desenvolvimento
Pseudomonas fluorescens/isolamento & purificação
Pseudomonas fluorescens/metabolismo
Pseudomonas fragi/crescimento & desenvolvimento
Pseudomonas fragi/isolamento & purificação
Pseudomonas fragi/metabolismo
Serratia liquefaciens/crescimento & desenvolvimento
Serratia liquefaciens/isolamento & purificação
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  3 / 28 MEDLINE  
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[PMID]:27339315
[Au] Autor:Mei YZ; Huang PW; Liu Y; He W; Fang WW
[Ad] Endereço:Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China. 08279@njnu.edu.cn.
[Ti] Título:Cold stress promoting a psychrotolerant bacterium Pseudomonas fragi P121 producing trehaloase.
[So] Source:World J Microbiol Biotechnol;32(8):134, 2016 Aug.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A newly isolated Pseudomonas fragi P121 strain in a soil sample taken from the Arctic Circle is able to produce trehalose. The P121 strain was able to grow at temperatures ranging from 4 to 25 °C, had an optimum pH of 6.5, and an optimum salt concentration of 2 %. The P121 strain had a survival rate of 29.1 % after being repeatedly frozen and thawed five times, and a survival rate of 78.9 % when placed in physiological saline for 15 days at 20 °C after cold shock, which is far higher than the type strain Pseudomonas fragi ATCC 4973. The P121 strain could produce 2.89 g/L trehalose, which was 18.6 % of dry cell weight within 52 h in a 25 L fermention tank using the malt extract prepared from barley as medium at 15 °C, while only 11.8 % of dry cell weight at 20 °C. These results suggested that cold stress promoted the strain producing trehalose. It is the first reported cold-tolerant bacterium that produces trehalose, which may protect cells against the cold environment.
[Mh] Termos MeSH primário: Pseudomonas fragi/crescimento & desenvolvimento
Pseudomonas fragi/isolamento & purificação
Trealose/metabolismo
[Mh] Termos MeSH secundário: Regiões Antárticas
Temperatura Baixa
Fermentação
Análise de Sequência de DNA
Microbiologia do Solo
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
B8WCK70T7I (Trehalose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-016-2097-1


  4 / 28 MEDLINE  
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[PMID]:26988396
[Au] Autor:Yanzhen M; Yang L; Xiangting X; Wei H
[Ad] Endereço:Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China. Electronic address: meiyanzhen@njnu.edu.cn.
[Ti] Título:Complete genome sequence of a bacterium Pseudomonas fragi P121, a strain with degradation of toxic compounds.
[So] Source:J Biotechnol;224:68-9, 2016 Apr 20.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A newly isolated strain P121 was identified as Pseudomonas fragi. The complete genome sequence of P.fragi P121 was carried out using the PacBio RSâ…¡ platform. The genome contains a circular chromosome with 5,101,809bp. The genome sequence suggests that the P121 exhibited the ability of degradation of toxic compounds. Genome sequencing information provides the genetic basis for the analysis of toxic compounds and the mechanism of extreme environmental adaptation of the strain.
[Mh] Termos MeSH primário: Genoma Bacteriano
Pseudomonas fragi/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Composição de Bases
Tamanho do Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160319
[St] Status:MEDLINE


  5 / 28 MEDLINE  
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[PMID]:26852809
[Au] Autor:Dugat-Bony E; Sarthou AS; Perello MC; de Revel G; Bonnarme P; Helinck S
[Ad] Endereço:Unite Mixte de Recherche, Génie et Microbiologie des Procédés Alimentaires, AgroParisTech, INRA, Université Paris-Saclay, 78850, Thiverval-Grignon, France.
[Ti] Título:The effect of reduced sodium chloride content on the microbiological and biochemical properties of a soft surface-ripened cheese.
[So] Source:J Dairy Sci;99(4):2502-2511, 2016 Apr.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many health authorities have targeted salt reduction in food products as a means to reduce dietary sodium intake due to the harmful effects associated with its excessive consumption. In the present work, we evaluated the effect of reducing sodium chloride (NaCl) content on the microbiological and biochemical characteristics of an experimental surface-ripened cheese. A control cheese (1.8% NaCl) and a cheese with a reduced NaCl content (1.3% NaCl) were sampled weekly over a period of 27d. Reducing NaCl content induced microbial perturbations such as the lesser development of the yeast Debaryomyces hansenii and the greater development of the gram-negative bacterium Hafnia alvei. This was accompanied by changes in proteolytic kinetics and in profiles of volatile aroma compounds and biogenic amine production. Finally, the development of the spoilage microorganism Pseudomonas fragi was significantly higher in the cheese with a reduced salt content.
[Mh] Termos MeSH primário: Queijo/microbiologia
Pseudomonas fragi/efeitos dos fármacos
Cloreto de Sódio na Dieta/farmacologia
Cloreto de Sódio/química
[Mh] Termos MeSH secundário: Animais
Aminas Biogênicas/análise
Queijo/análise
Cinética
Proteólise
Pseudomonas fragi/crescimento & desenvolvimento
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biogenic Amines); 0 (Sodium Chloride, Dietary); 0 (Volatile Organic Compounds); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170805
[Lr] Data última revisão:
170805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


  6 / 28 MEDLINE  
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[PMID]:25177730
[Au] Autor:Hernández-Hernández E; Regalado-González C; Vázquez-Landaverde P; Guerrero-Legarreta I; García-Almendárez BE
[Ad] Endereço:DIPA, PROPAC, Facultad de Química, Universidad Autónoma de Querétaro. C. U., Cerro de las Campanas s/n, Col. Las Campanas, 76010 Querétaro, QRO., Mexico.
[Ti] Título:Microencapsulation, chemical characterization, and antimicrobial activity of Mexican (Lippia graveolens H.B.K.) and European (Origanum vulgare L.) oregano essential oils.
[So] Source:ScientificWorldJournal;2014:641814, 2014.
[Is] ISSN:1537-744X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of solvent polarity (methanol and pentane) on the chemical composition of hydrodistilled essential oils (EO's) of Lippia graveolens H.B.K. (MXO) and Origanum vulgare L. (EUO) was studied by GC-MS. Composition of modified starch microencapsulated EO's was conducted by headspace-solid-phase microextraction (HS-SPME). The antimicrobial activity of free and microencapsulated EO's was evaluated. They were tested against Salmonella sp., Brochothrix thermosphacta, Pseudomonas fragi, Lactobacillus plantarum, and Micrococcus luteus. Thymol and carvacrol were among the main components of EO's and their free and microencapsulated inhibitory activity was tested against M. luteus, showing an additive combined effect. Chemical composition of EO's varied according to the solvent used for GC analysis and to volatile fraction as evaluated by HS-SPME. Thymol (both solvents) was the main component in essential oil of MXO, while carvacrol was the main component of the volatile fraction. EUO showed α-pinene (methanol) and γ-terpinene (pentane) as major constituents, the latter being the main component of the volatile fraction. EO's showed good stability after 3 months storage at 4°C, where antimicrobial activity of microencapsulated EO's remained the same, while free EO's decreased 41% (MXO) and 67% (EUO) from initial activity. Microencapsulation retains most antimicrobial activity and improves stability of EO's from oregano.
[Mh] Termos MeSH primário: Antibacterianos/química
Cápsulas/química
Lippia/química
Óleos Voláteis/química
Origanum/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Brochothrix/efeitos dos fármacos
Lactobacillus plantarum/efeitos dos fármacos
Micrococcus luteus/efeitos dos fármacos
Monoterpenos/análise
Monoterpenos/farmacologia
Óleos Voláteis/farmacologia
Pseudomonas fragi/efeitos dos fármacos
Salmonella/efeitos dos fármacos
Amido/química
Timol/análise
Timol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Capsules); 0 (Monoterpenes); 0 (Oils, Volatile); 3J50XA376E (Thymol); 4YGF4PQP49 (gamma-terpinene); 9005-25-8 (Starch); 9B1J4V995Q (carvacrol); JPF3YI7O34 (alpha-pinene)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:150804
[Lr] Data última revisão:
150804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140902
[St] Status:MEDLINE
[do] DOI:10.1155/2014/641814


  7 / 28 MEDLINE  
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[PMID]:23496842
[Au] Autor:Venkatesan R; Wierenga RK
[Ad] Endereço:Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu 90014, Finland.
[Ti] Título:Structure of mycobacterial ß-oxidation trifunctional enzyme reveals its altered assembly and putative substrate channeling pathway.
[So] Source:ACS Chem Biol;8(5):1063-73, 2013 May 17.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The incidence of tuberculosis is increasing due to the appearance of new drug-resistant variants. A thorough understanding of the disease organism is essential in order to create more effective drugs. In an attempt to understand better the poorly studied lipid metabolism of Mycobacterium tuberculosis (Mtb), we identified and characterized its fatty acid ß-oxidation complex (trifunctional enzyme (TFE)). TFE is an α(2)ß(2) complex consisting of two types of polypeptides catalyzing three of the four reactions of the ß-oxidation of fatty acids. The kinetic constants (k(cat) and K(m)) show that the complexed α chain is more active than the individual α chain. Crystal structures of Mtb TFE (mtTFE) reveal that the quaternary assembly is strikingly different from the already known Pseudomonas fragi TFE (pfTFE) assembly due to the presence of a helical insertion (LA5) in the mtTFE-ß subunit. This helical insertion prevents the pfTFE mode of assembly, as it would clash with helix H9A of the TFE-α chain. The mtTFE assembly appears to be more rigid and results in a different substrate channeling path between the α and the ß subunits. Structural comparisons suggest that the mtTFE active sites can accommodate bulkier fatty acyl chains than in pfTFE. Although another thiolase (FadA2), more closely related to human TFE-ß/thiolase, is present in the Mtb genome, it does not form a complex with mtTFE-α. Extensive phylogenetic analyses show that there are at least four TFE subfamilies. Our studies highlight the molecular properties of mtTFE, significantly extending the structural knowledge on this type of very interesting multifunctional enzymes.
[Mh] Termos MeSH primário: 3-Hidroxiacil-CoA Desidrogenases/química
3-Hidroxiacil-CoA Desidrogenases/metabolismo
Acetil-CoA C-Aciltransferase/química
Acetil-CoA C-Aciltransferase/metabolismo
Isomerases de Ligação Dupla Carbono-Carbono/química
Isomerases de Ligação Dupla Carbono-Carbono/metabolismo
Enoil-CoA Hidratase/química
Enoil-CoA Hidratase/metabolismo
Mycobacterium tuberculosis/metabolismo
Filogenia
Racemases e Epimerases/química
Racemases e Epimerases/metabolismo
[Mh] Termos MeSH secundário: Acil Coenzima A/metabolismo
Sequência de Aminoácidos
Domínio Catalítico
Cristalografia por Raios X
Cinética
Ligantes
Dados de Sequência Molecular
Mycobacterium tuberculosis/enzimologia
Oxirredução
Conformação Proteica
Pseudomonas fragi/enzimologia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-decenoyl-coenzyme A); 0 (Acyl Coenzyme A); 0 (Ligands); 0 (fatty acid oxidation complex); EC 1.1.1.- (3-Hydroxyacyl CoA Dehydrogenases); EC 2.3.1.16 (Acetyl-CoA C-Acyltransferase); EC 4.2.1.17 (Enoyl-CoA Hydratase); EC 5.1.- (Racemases and Epimerases); EC 5.3.3.- (Carbon-Carbon Double Bond Isomerases)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:130517
[Lr] Data última revisão:
130517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130319
[St] Status:MEDLINE
[do] DOI:10.1021/cb400007k


  8 / 28 MEDLINE  
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[PMID]:22686012
[Au] Autor:Balko AB; Avdeeva LV
[Ti] Título:[Screening of producers of bacteriocin-like substances active against Pseudomonas aeruginosa].
[So] Source:Mikrobiol Z;74(2):8-13, 2012 Mar-Apr.
[Is] ISSN:1028-0987
[Cp] País de publicação:Ukraine
[La] Idioma:rus
[Ab] Resumo:The strains (n = 94) of 16 Pseudomonas species have been screened for producers of substances active against Pseudomonas aeruginosa. Investigated cultures were divided into two groups. The majority of Pseudomonas species have been included in the first group. These species were able to produce substances with low and medium activity spectrum. In the first group P. mendocina, P. fragi and P. taetrolens lysates were the most active and influenced 30-50% of indicator cultures. Only P. aeruginosa strains belong to the second group. The microorganisms of this group were able to produce substances with considerably higher activity spectrum. Among all investigated pseudomonades four P. aeruginosa strain lysates possessed the highest activity and were active against more than 75% of used cultures. It was shown that the main active killer components of these lysates belonged to low-weight pyocins.
[Mh] Termos MeSH primário: Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas/metabolismo
Piocinas
[Mh] Termos MeSH secundário: Permeabilidade da Membrana Celular
Ensaios de Triagem em Larga Escala
Testes de Sensibilidade Microbiana
Peso Molecular
Pseudomonas/classificação
Pseudomonas/isolamento & purificação
Pseudomonas aeruginosa/fisiologia
Pseudomonas fragi/metabolismo
Piocinas/biossíntese
Piocinas/farmacologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pyocins)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:120612
[Lr] Data última revisão:
120612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120613
[St] Status:MEDLINE


  9 / 28 MEDLINE  
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[PMID]:22628506
[Au] Autor:Mei Y; Sun Y; He J; Wang Q; Sun Y; Shao W
[Ad] Endereço:College of Life Science, Nanjing Normal University, Nanjing, Jiangsu, China.
[Ti] Título:Genome sequences of Pseudomonas fragi strains A22 and B25.
[So] Source:J Bacteriol;194(12):3276-7, 2012 Jun.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas fragi A22 is a novel isolate that produces bead-like particles (A22B) in its cell wall. To explore the genetic basis for the formation of A22B, P. fragi A22 and the type strain of the species, P. fragi B25, were subjected to genome sequence analysis. Here, we report the draft genome sequences and automatic annotation of both strains. These data offer a solid base for related studies of P. fragi, including comparative genomics, proteomics, and gene mining.
[Mh] Termos MeSH primário: DNA Bacteriano/química
DNA Bacteriano/genética
Genoma Bacteriano
Pseudomonas fragi/genética
[Mh] Termos MeSH secundário: Parede Celular/ultraestrutura
Dados de Sequência Molecular
Pseudomonas fragi/ultraestrutura
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120526
[St] Status:MEDLINE
[do] DOI:10.1128/JB.00488-12


  10 / 28 MEDLINE  
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[PMID]:22112959
[Au] Autor:Sharma A; Bhattacharya A; Shrivastava A
[Ad] Endereço:Bacteriology Laboratory, Department of P.G. Studies and Research in Biological Science, Rani Durgavati University, Pachpedi, Jabalpur 482001, Madhya Pradesh, India. anjoo1999@gmail.com
[Ti] Título:Biomimetic CO2 sequestration using purified carbonic anhydrase from indigenous bacterial strains immobilized on biopolymeric materials.
[So] Source:Enzyme Microb Technol;48(4-5):416-26, 2011 Apr 07.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study deals with immobilization of purified CA and whole cell of Pseudomonas fragi, Micrococcus lylae, and Micrococcus luteus 2 on different biopolymer matrices. Highest enzyme immobilization was achieved with P. fragi CA (89%) on chitosan-KOH beads, while maximum cell immobilization was achieved with M. lylae (75%) on chitosan-NH(4)OH beads. A maximum increase of 1.08-1.18 fold stability between 35 and 55°C was observed for M. lylae immobilized CA. The storage stability was improved by 2.02 folds after immobilization. FTIR spectra confirmed the adsorption of CA on chitosan-KOH beads following hydrophilic interactions. Calcium carbonate precipitation was achieved using chitosan-KOH immobilized P. fragi CA. More than 2 fold increase in sequestration potential was observed for immobilized system as compared to free enzyme. XRD spectra revealed calcite as the dominant phase in biomimetically produced calcium carbonate.
[Mh] Termos MeSH primário: Materiais Biomiméticos/metabolismo
Dióxido de Carbono/metabolismo
Anidrases Carbônicas/metabolismo
Células Imobilizadas/enzimologia
Enzimas Imobilizadas/metabolismo
Pseudomonas fragi/enzimologia
[Mh] Termos MeSH secundário: Alginatos/química
Biotecnologia/métodos
Carbonato de Cálcio/metabolismo
Anidrases Carbônicas/química
Anidrases Carbônicas/isolamento & purificação
Quitosana/química
Enzimas Imobilizadas/química
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Micrococcus/enzimologia
Micrococcus luteus/enzimologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alginates); 0 (Enzymes, Immobilized); 0 (Hexuronic Acids); 142M471B3J (Carbon Dioxide); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9012-76-4 (Chitosan); EC 4.2.1.1 (Carbonic Anhydrases); H0G9379FGK (Calcium Carbonate)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111125
[St] Status:MEDLINE
[do] DOI:10.1016/j.enzmictec.2011.02.001



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