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  1 / 1135 MEDLINE  
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[PMID]:29447189
[Au] Autor:Saleem AR; Brunetti C; Khalid A; Della Rocca G; Raio A; Emiliani G; De Carlo A; Mahmood T; Centritto M
[Ad] Endereço:Department of Earth and Environmental Sciences, Bahria University Islamabad Campus, Islamabad, Pakistan.
[Ti] Título:Drought response of Mucuna pruriens (L.) DC. inoculated with ACC deaminase and IAA producing rhizobacteria.
[So] Source:PLoS One;13(2):e0191218, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drought is one of the major constraints limiting agricultural production worldwide and is expected to increase in the future. Limited water availability causes significant effects to plant growth and physiology. Plants have evolved different traits to mitigate the stress imposed by drought. The presence of plant growth-promoting rhizobacteria (PGPR) could play an important role in improving plant performances and productivity under drought. These beneficial microorganisms colonize the rhizosphere of plants and increase drought tolerance by lowering ethylene formation. In the present study, we demonstrate the potential to improve the growth of velvet bean under water deficit conditions of two different strains of PGPR with ACCd (1-Aminocyclopropane-1-Carboxylate deaminase) activity isolated from rainfed farming system. We compared uninoculated and inoculated plants with PGPR to assess: a) photosynthetic performance and biomass; b) ACC content and ethylene emission from leaves and roots; c) leaf isoprene emission. Our results provided evidence that under drought conditions inoculation with PGPR containing the ACCd enzyme could improve plant growth compared to untreated plants. Ethylene emission from roots and leaves of inoculated velvet bean plants was significantly lower than uninoculated plants. Moreover, isoprene emission increased with drought stress progression and was higher in inoculated plants compared to uninoculated counterparts. These findings clearly illustrate that selected PGPR strains isolated from rainfed areas could be highly effective in promoting plant growth under drought conditions by decreasing ACC and ethylene levels in plants.
[Mh] Termos MeSH primário: Mucuna/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Rhizobiaceae/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Carbono-Carbono Liases/metabolismo
Secas
Etilenos/biossíntese
Mucuna/fisiologia
Fotossíntese
Desenvolvimento Vegetal
Reguladores de Crescimento de Planta/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Rhizobiaceae/genética
Rizosfera
Microbiologia do Solo
Água/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ethylenes); 0 (Plant Growth Regulators); 059QF0KO0R (Water); 91GW059KN7 (ethylene); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191218


  2 / 1135 MEDLINE  
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[PMID]:29220441
[Au] Autor:Saha S; Hosmani PS; Villalobos-Ayala K; Miller S; Shippy T; Flores M; Rosendale A; Cordola C; Bell T; Mann H; DeAvila G; DeAvila D; Moore Z; Buller K; Ciolkevich K; Nandyal S; Mahoney R; Van Voorhis J; Dunlevy M; Farrow D; Hunter D; Morgan T; Shore K; Guzman V; Izsak A; Dixon DE; Cridge A; Cano L; Cao X; Jiang H; Leng N; Johnson S; Cantarel BL; Richards S; English A; Shatters RG; Childers C; Chen MJ; Hunter W; Cilia M; Mueller LA; Munoz-Torres M; Nelson D; Poelchau MF; Benoit JB; Wiersma-Koch H; D'Elia T; Brown SJ
[Ad] Endereço:Boyce Thompson Institute, Ithaca, NY 14853.
[Ti] Título:Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community.
[So] Source:Database (Oxford);2017, 2017 Jan 01.
[Is] ISSN:1758-0463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Database URL: https://citrusgreening.org/.
[Mh] Termos MeSH primário: Bases de Dados Genéticas
Genoma de Inseto/genética
Hemípteros
Insetos Vetores/genética
Controle de Pragas
Doenças das Plantas
[Mh] Termos MeSH secundário: Animais
Citrus/microbiologia
Citrus/parasitologia
Hemípteros/genética
Hemípteros/microbiologia
Rhizobiaceae
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1093/database/bax032


  3 / 1135 MEDLINE  
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[PMID]:29328677
[Au] Autor:Suh JH; Niu YS; Wang Z; Gmitter FG; Wang Y
[Ti] Título:Metabolic Analysis Reveals Altered Long-Chain Fatty Acid Metabolism in the Host by Huanglongbing Disease.
[So] Source:J Agric Food Chem;66(5):1296-1304, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Candidatus Liberibacter asiaticus (CLas) is the presumed causal agent of Huanglongbing, one of the most destructive diseases in citrus. However, the lipid metabolism component of host response to this pathogen has not been investigated well. Here, metabolic profiling of a variety of long-chain fatty acids and their oxidation products was first performed to elucidate altered host metabolic responses of disease. Fatty acid signals were found to decrease obviously in response to disease regardless of cultivar. Several lipid oxidation products strongly correlated with those fatty acids were also consistently reduced in the diseased group. Using a series of statistical methods and metabolic pathway mapping, we found significant markers contributing to the pathological symptoms and identified their internal relationships and metabolic network. Our findings suggest that the infection of CLas may cause the altered metabolism of long-chain fatty acids, possibly leading to manipulation of the host's defense derived from fatty acids.
[Mh] Termos MeSH primário: Citrus/metabolismo
Citrus/microbiologia
Ácidos Graxos/metabolismo
Doenças das Plantas/microbiologia
Rhizobiaceae
[Mh] Termos MeSH secundário: Citrus paradisi/metabolismo
Citrus paradisi/microbiologia
Citrus sinensis/metabolismo
Citrus sinensis/microbiologia
Florida
Peroxidação de Lipídeos
Metabolômica
Folhas de Planta/metabolismo
Folhas de Planta/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05273


  4 / 1135 MEDLINE  
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[PMID]:28992475
[Au] Autor:Akhtar MJ; Ullah S; Ahmad I; Rauf A; Nadeem SM; Khan MY; Hussain S; Bulgariu L
[Ad] Endereço:Institute of Soil & Environmental Sciences, University of Agriculture Faisalabad, 38040, Pakistan.
[Ti] Título:Nickel phytoextraction through bacterial inoculation in Raphanus sativus.
[So] Source:Chemosphere;190:234-242, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A pot experiment was conducted to evaluate the potential of two plant growth promoting rhizobacteria (PGPR) viz. Bacillus sp. CIK-516 and Stenotrophomonas sp. CIK-517Y for improving the growth and Ni uptake of radish (Raphanus sativus) in the presence of four different levels of Ni contamination (0, 50, 100, 150 mg Ni kg soil). Plant growth, dry biomass, chlorophyll and nitrogen contents were significantly reduced by the exogenous application of Ni, however, bacterial inoculation diluted the negative impacts of Ni stress on radish by improving these parameters. PGPR strain CIK-516 increased root length (9-27%), shoot length (8-27%), root dry biomass (2-32%), shoot dry biomass (9-51%), root girth (6-48%), total chlorophyll (4-38%) and shoot nitrogen contents (11-15%) in Ni contaminated and non-contaminated soils. Positive regulation of chlorophyll and nitrogen contents by the inoculated plants shows plant tolerance mechanism of Ni stress. Bacterial strain (CIK-516) exhibited indole acetic acid and 1-amino-cyclopropane-1-carboxylate deaminase potentials which would have helped radish plant to stabilize in Ni contaminated soil and thereby increased Ni uptake (24-257 in shoot and 58-609 in root mg kg dry biomass) and facilitated accumulation in radish (bioaccumulation factor = 0.6-1.7) depending upon soil Ni contamination. Based on the findings of this study, it might be suggested that inoculation with bacterial strain CIK-516 could be an efficient tool for enhanced Ni phytoextraction in radish.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Níquel/isolamento & purificação
Raphanus/microbiologia
Poluentes do Solo/isolamento & purificação
[Mh] Termos MeSH secundário: Inoculantes Agrícolas
Bacillus/metabolismo
Clorofila/análise
Níquel/farmacologia
Nitrogênio/análise
Desenvolvimento Vegetal/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Raphanus/crescimento & desenvolvimento
Rhizobiaceae/metabolismo
Poluentes do Solo/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 1406-65-1 (Chlorophyll); 7OV03QG267 (Nickel); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  5 / 1135 MEDLINE  
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[PMID]:29297848
[Au] Autor:Siddiqi MZ; Choi GM; Im WT
[Ad] Endereço:1​Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.
[Ti] Título:Ciceribacter azotifigens sp. nov., a nitrogen-fixing bacterium isolated from activated sludge.
[So] Source:Int J Syst Evol Microbiol;68(2):482-486, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-reaction-negative, catalase- and oxidase-positive, aerobic, transparent, motile and rod-shaped bacterium that was capable of fixing dinitrogen (designated strain A.slu09 ), isolated from activated sludge, was characterized by a polyphasic approach to clarify its taxonomic position. Strain A.slu09 was observed to grow optimally at 30 °C and at pH 7.0 on R2A agar medium. Strain A.slu09 showed ß-glucosidase activity, converting the major ginsenoside Rd to ginsenoside F2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A.slu09 belongs to the genus Ciceribacter of the family Rhizobiaceae and was most closely related to Ciceribacter lividus MSSRFBL1 (97.8 % similarity). The DNA G+C content was 67.2 mol%. The DNA-DNA hybridization value between strain A.slu09 and C. lividus KCTC 32403 was 16.9±1.17 %. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, aminophospholipid and two glycolipids, and one unknown phospholipid as a minor lipid. The predominant quinone was ubiquinone-10 (Q-10). The major fatty acids were C19 : 0 cyclo ω8c, C18 : 1 ω7c and/or C18 : 1ω6c (summed feature 8) and C18 : 0, a profile that supported the affiliation of A.slu09 to the genus Ciceribacter. Moreover, the physiological and biochemical characteristics and low level of DNA-DNA relatedness allowed the phenotypic and genotypic differentiation of strain A.slu09 from the recognized species of the genus Ciceribacter. Therefore, strain A.slu09 represents a novel species of the genus Ciceribacter, for which the name Ciceribacter azotifigens sp. nov. is proposed. The type strain is A.slu09 (=KACC 19080 =LMG 29962 ).
[Mh] Termos MeSH primário: Fixação de Nitrogênio
Filogenia
Rhizobiaceae/classificação
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Ginsenosídeos
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Rhizobiaceae/genética
Rhizobiaceae/isolamento & purificação
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Ginsenosides); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Sewage); 0 (ginsenoside F2); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2); WB232T95AV (ginsenoside Rd)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002438


  6 / 1135 MEDLINE  
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[PMID]:29236706
[Au] Autor:Sétamou M; Alabi OJ; Simpson CR; Jifon JL
[Ad] Endereço:Texas A&M University-Kingsville Citrus Center, Weslaco, United States of America.
[Ti] Título:Contrasting amino acid profiles among permissive and non-permissive hosts of Candidatus Liberibacter asiaticus, putative causal agent of Huanglongbing.
[So] Source:PLoS One;12(12):e0187921, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Huanglongbing is a devastating disease of citrus. In this study, a comprehensive profile of phloem sap amino acids (AA) in four permissive host plants of Candidatus Liberibacter asiaticus (CLas) and three non-permissive Rutaceae plants was conducted to gain a better understanding of host factors that may promote or suppress the bacterium. The AA profiles of Diaphorina citri nymphs and adults were similarly analyzed. A total of 38 unique AAs were detected in phloem sap of the various plants and D. citri samples, with phloem sap of young shoots containing more AAs and at higher concentrations than their mature counterparts. All AAs detected in phloem sap of non-permissive plants were also present in CLas -permissive hosts plus additional AAs in the latter class of plants. However, the relative composition of 18 commonly shared AAs varied between CLas -permissive hosts and non-permissive plants. Multivariate analysis with a partial least square discriminant methodology revealed a total of 12 AAs as major factors affecting CLas host status, of which seven were positively related to CLas tolerance/resistance and five positively associated with CLas susceptibility. Most of the AAs positively associated with CLas susceptibility were predominantly of the glutamate family, notably stressed-induced AAs such as arginine, GABA and proline. In contrast, AAs positively correlated with CLas tolerance/resistance were mainly of the serine family. Further analysis revealed that whereas the relative proportions of AAs positively associated with CLas susceptibility did not vary with host developmental stages, those associated with CLas tolerance/resistance increased with flush shoot maturity. Significantly, the proline-to-glycine ratio was determined to be an important discriminating factor for CLas permissivity with higher values characteristic of CLas -permissive hosts. This ratio could be exploited as a biomarker in HLB-resistance breeding programs.
[Mh] Termos MeSH primário: Aminoácidos/química
Citrus/microbiologia
Doenças das Plantas/microbiologia
Rhizobiaceae/química
[Mh] Termos MeSH secundário: Aminoácidos/isolamento & purificação
Floema
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187921


  7 / 1135 MEDLINE  
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[PMID]:29232716
[Au] Autor:Hu Y; Zhong X; Liu X; Lou B; Zhou C; Wang X
[Ad] Endereço:National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, P. R. China.
[Ti] Título:Comparative transcriptome analysis unveils the tolerance mechanisms of Citrus hystrix in response to 'Candidatus Liberibacter asiaticus' infection.
[So] Source:PLoS One;12(12):e0189229, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Citrus Huanglongbing (HLB), a highly devastating citrus disease, is associated with 'Candidatus Liberibacter asiacitus' (CLas), a member of phloem-inhabiting α-proteobacteria. HLB can affect all cultivated citrus and no cure is currently available. Previous studies showed that Kaffir lime (Citrus hystrix), primarily grown in South Asia and Southeast Asia, was tolerant to HLB but the molecular mechanism remains unknown. In this study, gene expression profiling experiments were performed on HLB-tolerant C. hystrix and HLB-susceptible C. sinensis three months after inoculation with CLas using RNA-seq data. Differentially expressed genes (DEGs) in the two citrus cultivars were mainly involved in diverse cellular functions including carbohydrate metabolism, photosynthesis, cell wall metabolism, secondary metabolism, hormone metabolism and oxidation/reduction processes. Notably, starch synthesis and photosynthesis process were not disturbed in CLas-infected C. hystrix. Most of the DEGs involved in cell wall metabolism and secondary metabolism were up-regulated in C. hystrix. In addition, the activation of peroxidases, Cu/Zn-SOD and POD4, may also enhance the tolerance of C. hystrix to CLas. This study provides an insight into the host response of HLB-tolerant citrus cultivar to CLas. C. hystrix is potentially useful for HLB-tolerant/resistant citrus breeding in the future.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Citrus/fisiologia
Doenças das Plantas/microbiologia
Rhizobiaceae/patogenicidade
Transcriptoma
[Mh] Termos MeSH secundário: Metabolismo dos Carboidratos
Citrus/metabolismo
Citrus/microbiologia
Fotossíntese
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189229


  8 / 1135 MEDLINE  
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[PMID]:28686606
[Au] Autor:Hegazi NA; Sarhan MS; Fayez M; Patz S; Murphy BR; Ruppel S
[Ad] Endereço:Environmental Studies and Research Unit (ESRU), Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, Egypt.
[Ti] Título:Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.
[So] Source:PLoS One;12(7):e0180424, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne (Medicago sativa L.), we propose plant-only teabags culture media to mimic the nutritional matrix available in the rhizosphere. Here, we show that culture media prepared from Lucerne powder teabags substantially increased the cultivability of Lucerne rhizobacteria compared with a standard nutrient agar, where we found that the cultivable populations significantly increased by up to 60% of the total bacterial numbers as estimated by Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Cluster analysis of 16S rDNA Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) of cultivable Colony-Forming Units (CFUs) revealed a more distinct composition and separation of bacterial populations recovered on the plant-only teabags culture media than those developed on a standard nutrient agar. Further, the new plant medium gave preference to the micro-symbiont Sinorhizobium meliloti, and succeeded in isolating a number of not-yet-cultured bacteria, most closely matched to Novosphingobium sp., Lysobacter sp. and Pedobacter sp. The present study may encourage other researchers to consider moving from the well-established standard culture media to the challenging new plant-only culture media. Such a move may reveal previously hidden members of rhizobacteria, and help to further explore their potential environmental impacts.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/métodos
Medicago sativa/microbiologia
Rhizobiaceae/crescimento & desenvolvimento
Microbiologia do Solo
[Mh] Termos MeSH secundário: Meios de Cultura/farmacologia
Ecossistema
Lysobacter/efeitos dos fármacos
Lysobacter/crescimento & desenvolvimento
Pedobacter/efeitos dos fármacos
Pedobacter/crescimento & desenvolvimento
RNA Ribossômico 16S/genética
Rhizobiaceae/efeitos dos fármacos
Rizosfera
Sinorhizobium meliloti/efeitos dos fármacos
Sinorhizobium meliloti/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180424


  9 / 1135 MEDLINE  
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[PMID]:28632769
[Au] Autor:Kruse A; Fattah-Hosseini S; Saha S; Johnson R; Warwick E; Sturgeon K; Mueller L; MacCoss MJ; Shatters RG; Cilia Heck M
[Ad] Endereço:Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Sciences, Cornell University, Ithaca, New York, United States of America.
[Ti] Título:Combining 'omics and microscopy to visualize interactions between the Asian citrus psyllid vector and the Huanglongbing pathogen Candidatus Liberibacter asiaticus in the insect gut.
[So] Source:PLoS One;12(6):e0179531, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Huanglongbing, or citrus greening disease, is an economically devastating bacterial disease of citrus. It is associated with infection by the gram-negative bacterium Candidatus Liberibacter asiaticus (CLas). CLas is transmitted by Diaphorina citri, the Asian citrus psyllid (ACP). For insect transmission to occur, CLas must be ingested during feeding on infected phloem sap and cross the gut barrier to gain entry into the insect vector. To investigate the effects of CLas exposure at the gut-pathogen interface, we performed RNAseq and mass spectrometry-based proteomics to analyze the transcriptome and proteome, respectively, of ACP gut tissue. CLas exposure resulted in changes in pathways involving the TCA cycle, iron metabolism, insecticide resistance and the insect's immune system. We identified 83 long non-coding RNAs that are responsive to CLas, two of which appear to be specific to the ACP. Proteomics analysis also enabled us to determine that Wolbachia, a symbiont of the ACP, undergoes proteome regulation when CLas is present. Fluorescent in situ hybridization (FISH) confirmed that Wolbachia and CLas inhabit the same ACP gut cells, but do not co-localize within those cells. Wolbachia cells are prevalent throughout the gut epithelial cell cytoplasm, and Wolbachia titer is more variable in the guts of CLas exposed insects. CLas is detected on the luminal membrane, in puncta within the gut epithelial cell cytoplasm, along actin filaments in the gut visceral muscles, and rarely, in association with gut cell nuclei. Our study provides a snapshot of how the psyllid gut copes with CLas exposure and provides information on pathways and proteins for targeted disruption of CLas-vector interactions at the gut interface.
[Mh] Termos MeSH primário: Hemípteros/microbiologia
Proteoma/análise
Proteômica
Rhizobiaceae/fisiologia
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Ciclo do Ácido Cítrico/genética
Citrus/microbiologia
Sistema Digestório/metabolismo
Sistema Digestório/microbiologia
Regulação para Baixo
Hemípteros/genética
Insetos Vetores/microbiologia
Microscopia Confocal
Mitocôndrias/metabolismo
Doenças das Plantas/microbiologia
RNA/química
RNA/isolamento & purificação
RNA/metabolismo
Análise de Sequência de RNA
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); 63231-63-0 (RNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179531


  10 / 1135 MEDLINE  
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[PMID]:28516945
[Au] Autor:Ledford H
[Ti] Título:Geneticists enlist engineered virus and CRISPR to battle citrus disease.
[So] Source:Nature;545(7654):277-278, 2017 05 16.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Terapia Biológica/métodos
Sistemas CRISPR-Cas/genética
Citrus sinensis
Edição de Genes
Doenças das Plantas/microbiologia
Doenças das Plantas/prevenção & controle
Vírus de Plantas/patogenicidade
Rhizobiaceae/virologia
[Mh] Termos MeSH secundário: Citrus sinensis/genética
Citrus sinensis/microbiologia
Citrus sinensis/virologia
Resistência à Doença/genética
Florida
Indústria Alimentícia
Alimentos Geneticamente Modificados/microbiologia
Alimentos Geneticamente Modificados/virologia
Genes de Plantas/genética
Doenças das Plantas/genética
Doenças das Plantas/virologia
Rhizobiaceae/patogenicidade
Spinacia oleracea/genética
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1038/545277a



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