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  1 / 6083 MEDLINE  
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[PMID]:28895521
[Au] Autor:Cordeiro AB; Ribeiro RA; Helene LCF; Hungria M
[Ad] Endereço:1​Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
[Ti] Título:Rhizobium esperanzae sp. nov., a N2-fixing root symbiont of Phaseolus vulgaris from Mexican soils.
[So] Source:Int J Syst Evol Microbiol;67(10):3937-3945, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Common bean (Phaseolus vulgaris L.) is the most important legume consumed worldwide; its genetic origins lie in the Mesoamerican (main centre) and Andean regions. It is promiscuous in establishing root-nodule symbioses; however, in the centres of origin/domestication, the predominant association is with Rhizobium etli. We have previously identified a new lineage (PEL-3) comprising three strains (CNPSo 661, CNPSo 666 and CNPSo 668 ) isolated from root nodules of common bean in Mexico, and that have now been analysed in more detail. Sequences of the 16S rRNA gene positioned the three strains in a large clade including R. etli. Multilocus sequence analysis (MLSA) with four housekeeping genes (recA, glnII, gyrB and rpoA) positioned the three strains in a clade distinct from all other described species, with 100 % bootstrap support, and nucleotide identity (NI) of the four concatenated genes with the closest species R. etli was 95.0 %. Average nucleotide identity (ANI) values of the whole genome of CNPSo 668 and the closest species, R. etli, was 92.9 %. In the analyses of the symbiotic genes nifH and nodC, the strains comprised a cluster with other rhizobial symbionts of P. vulgaris. Other phenotypic and genotypic traits were determined for the new group and our data support the description of the three CNPSo strains as a novel species, for which the name Rhizobium esperanzae is proposed. The type strain is CNPSo 668 (=UMR 1320 =Z87-8 =LMG 30030 =U 10001 ), isolated from a common-bean nodule in Mexico.
[Mh] Termos MeSH primário: Fixação de Nitrogênio
Phaseolus/microbiologia
Filogenia
Raízes de Plantas/microbiologia
Rhizobium/classificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
México
Tipagem de Sequências Multilocus
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Rhizobium/genética
Rhizobium/isolamento & purificação
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002225


  2 / 6083 MEDLINE  
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[PMID]:28820092
[Au] Autor:Gao JL; Sun P; Wang XM; Lv FY; Mao XJ; Sun JG
[Ad] Endereço:1​Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China.
[Ti] Título:Rhizobium wenxiniae sp. nov., an endophytic bacterium isolated from maize root.
[So] Source:Int J Syst Evol Microbiol;67(8):2798-2803, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel Gram-stain-negative, aerobic, rod-shaped strain designated 166T was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, PR China. The 16S rRNA gene sequence analysis indicated that strain 166T belongs to the genus Rhizobium and is closely related to Rhizobium cellulosilyticum ALA10B2T and Rhizobium yantingense H66T with sequence similarities of 98.8 and 98.3 %, respectively. According to atpD and recA sequence analysis, the highest sequence similarity between strain 166T and R. cellulosilyticum ALA10B2T is 93.8 and 84.7 %, respectively. However, the new isolate exhibited relatively low levels of DNA-DNA relatedness with respect to R. cellulosilyticum DSM 18291T (20.8±2.3 %) and Rhizobium yantingense CCTCC AB 2014007T (47.2±1.4 %). The DNA G+C content of strain 166T was 59.8 mol%. The main polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an unidentified aminophospholipid and an unidentified aminolipid. The major fatty acids of strain 166T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 166T from the type strains of closely related species, R. cellulosilyticum DSM 18291T and R. yantingense CCTCC AB 2014007T. Strain 166T represents a novel species within the genus Rhizobium, for which the name Rhizobium wenxiniae sp. nov. is proposed, with the type strain 166T (=CGMCC 1.15279T=DSM 100734T).
[Mh] Termos MeSH primário: Filogenia
Raízes de Plantas/microbiologia
Rhizobium/classificação
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Pequim
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Rhizobium/genética
Rhizobium/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002025


  3 / 6083 MEDLINE  
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[PMID]:28771548
[Au] Autor:Nanjareddy K; Arthikala MK; Gómez BM; Blanco L; Lara M
[Ad] Endereço:Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León- Universidad Nacional Autónoma de México (UNAM), León, Guanajuato, México.
[Ti] Título:Differentially expressed genes in mycorrhized and nodulated roots of common bean are associated with defense, cell wall architecture, N metabolism, and P metabolism.
[So] Source:PLoS One;12(8):e0182328, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Legumes participate in two important endosymbiotic associations, with phosphorus-acquiring arbuscular mycorrhiza (AM, soil fungi) and with nitrogen-fixing bacterial rhizobia. These divergent symbionts share a common symbiotic signal transduction pathway that facilitates the establishment of mycorrhization and nodulation in legumes. However, the unique and shared downstream genes essential for AM and nodule development have not been identified in crop legumes. Here, we used ion torrent next-generation sequencing to perform comparative transcriptomics of common bean (Phaseolus vulgaris) roots colonized by AM or rhizobia. We analyzed global gene expression profiles to identify unique and shared differentially expressed genes (DEGs) that regulate these two symbiotic interactions, and quantitatively compared DEG profiles. We identified 3,219 (1,959 upregulated and 1,260 downregulated) and 2,645 (1,247 upregulated and 1,398 downregulated) unigenes that were differentially expressed in response to mycorrhizal or rhizobial colonization, respectively, compared with uninoculated roots. We obtained quantitative expression profiles of unique and shared genes involved in processes related to defense, cell wall structure, N metabolism, and P metabolism in mycorrhized and nodulated roots. KEGG pathway analysis indicated that most genes involved in jasmonic acid and salicylic acid signaling, N metabolism, and inositol phosphate metabolism are variably expressed during symbiotic interactions. These combined data provide valuable information on symbiotic gene signaling networks that respond to mycorrhizal and rhizobial colonization, and serve as a guide for future genetic strategies to enhance P uptake and N-fixing capacity to increase the net yield of this valuable grain legume.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/genética
Micorrizas/genética
Micorrizas/metabolismo
Nitrogênio/metabolismo
Phaseolus/genética
Phaseolus/metabolismo
Fósforo/metabolismo
[Mh] Termos MeSH secundário: Parede Celular/química
Parede Celular/metabolismo
Ciclopentanos/metabolismo
Fungos/fisiologia
Perfilação da Expressão Gênica
Oxilipinas/metabolismo
Phaseolus/crescimento & desenvolvimento
Phaseolus/microbiologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
RNA Mensageiro/metabolismo
Rhizobium/fisiologia
Ácido Salicílico/metabolismo
Transdução de Sinais
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (RNA, Messenger); 27YLU75U4W (Phosphorus); 6RI5N05OWW (jasmonic acid); N762921K75 (Nitrogen); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182328


  4 / 6083 MEDLINE  
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[PMID]:28699873
[Au] Autor:Celador-Lera L; Menéndez E; Peix A; Igual JM; Velázquez E; Rivas R
[Ad] Endereço:1​Departamento de Microbiología y Genética and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Salamanca, Spain.
[Ti] Título:Rhizobium zeae sp. nov., isolated from maize (Zea mays L.) roots.
[So] Source:Int J Syst Evol Microbiol;67(7):2306-2311, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bacterial strain designated CRZM18RT was isolated from a root of Zea mays in Spain. The analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Rhizobium, with Rhizobium cellulosilyticum ALA10B2T and Rhizobium yantingense H66T being the most closely related species with 98.3 and 97.9 % sequence similarity, respectively. The analysis of the concatenated recA and atpD genes showed that strain CRZM18RT forms a cluster with these species and also with Rhizobiumsmilacinae PTYR-5T, but the recA and atpD genes of strain CRZM18RT were phylogenetically distant, with identities lower than 90 and 96 %, respectively. DNA-DNA hybridization analysis showed mean relatedness of 43, 22 and 38 % with respect to R. cellulosilyticum ALA10B2T, R. yantingense LMG 28229T and R. smilacinae LMG 27604T. Phenotypic characteristics also differed from those of the most closely related species of the genus Rhizobium. The major fatty acids were those from summed feature 8 (C18 : 1ω6c/C18 : 1ω7c) and C16 : 0. Based on the genotypic, chemotaxonomic and phenotypic data obtained in this study, we propose to classify strain CRZM18RT in a novel species named Rhizobium zeae sp. nov. (type strain CRZM18RT=LMG 29735T=CECT 9169T).
[Mh] Termos MeSH primário: Filogenia
Raízes de Plantas/microbiologia
Rhizobium/classificação
Zea mays/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Rhizobium/genética
Rhizobium/isolamento & purificação
Análise de Sequência de DNA
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001944


  5 / 6083 MEDLINE  
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[PMID]:28598309
[Au] Autor:Flores-Félix JD; Ramírez-Bahena MH; Salazar S; Peix A; Velázquez E
[Ad] Endereço:1​Departamento de Microbiología y Genética and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Salamanca, Spain.
[Ti] Título:Reclassification of Arthrobacter viscosus as Rhizobium viscosum comb. nov.
[So] Source:Int J Syst Evol Microbiol;67(6):1789-1792, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The species Arthrobacter viscosus was isolated from soil from Guatemala and it was classified into the genus Arthrobacter on the basis of phenotypic traits. Nevertheless, the results of16S rRNA gene analysis indicated that this species is a member of the genus Rhizobium, with Rhizobium alamii GBV016T and Rhizobium mesosinicum CCBAU 25010T as the most closely related species with 99.64 and 99.48 % similarity, respectively. The similarity values for the recA gene are 92.2 and 94.4 % with respect to R. alamii GBV016T and R. mesosinicum CCBAU 25010T, respectively, and those for the atpD gene are 92.9 and 98.7 %, respectively. Results of DNA-DNA hybridization analysis yield averages of 46 and 41 % relatedness with respect to the type strains of R. alamii and R. mesosinicum, respectively. Phenotypic characteristics also differed from those of the most closely related species of the genus Rhizobium. Therefore, based on the data obtained in this study, we propose to classify strain LMG 16473T as representing a novel species named Rhizobiumviscosum comb. nov. (type strain LMG 16473T=CECT 908T).
[Mh] Termos MeSH primário: Arthrobacter/classificação
Filogenia
Rhizobium/classificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Genes Bacterianos
Guatemala
Hibridização de Ácido Nucleico
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001864


  6 / 6083 MEDLINE  
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[PMID]:28375633
[Au] Autor:Chen J; Li Y; Wen S; Rosanoff A; Yang G; Sun X
[Ad] Endereço:College of Agro-Grassland Science, Nanjing Agricultural University , Nanjing 210095, People's Republic of China.
[Ti] Título:Magnesium Fertilizer-Induced Increase of Symbiotic Microorganisms Improves Forage Growth and Quality.
[So] Source:J Agric Food Chem;65(16):3253-3258, 2017 Apr 26.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Magnesium (Mg) plays important roles in photosynthesis and protein synthesis; however, latent Mg deficiencies are common phenomena that can influence food quality. Nevertheless, the effects of Mg fertilizer additions on plant carbon (C):nitrogen (N):phosphorus (P) stoichiometry, an important index of food quality, are unclear and the underlying mechanisms unexplored. We conducted a greenhouse experiment using low-Mg in situ soil without and with a gradient of Mg additions to investigate the effect of Mg fertilizer on growth and stoichiometry of maize and soybean and also measure these plants' main symbiotic microorganisms: arbuscular mycorrhizal fungi (AMF) and rhizobium, respectively. Our results showed that Mg addition significantly improved both plant species' growth and also increased N and P concentrations in soybean and maize, respectively, resulting in low C:N ratio and high N:P ratio in soybean and low C:P and N:P ratios in maize. These results presumably stemmed from the increase of nutrients supplied by activation-enhanced plant symbiotic microorganisms, an explanation supported by statistically significant positive correlations between plant stoichiometry and plants' symbiotic microorganisms' increased growth with Mg addition. We conclude that Mg supply can improve plant growth and alter plant stoichiometry via enhanced activity of plant symbiotic microorganisms. Possible mechanisms underlying this positive plant-soil feedback include an enhanced photosynthetic product flow to roots caused by adequate Mg supply.
[Mh] Termos MeSH primário: Fertilizantes/análise
Magnésio/metabolismo
Micorrizas/metabolismo
Rhizobium/metabolismo
Feijão de Soja/crescimento & desenvolvimento
Feijão de Soja/microbiologia
Simbiose
Zea mays/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Magnésio/análise
Micorrizas/crescimento & desenvolvimento
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Rhizobium/crescimento & desenvolvimento
Microbiologia do Solo
Feijão de Soja/química
Feijão de Soja/fisiologia
Zea mays/química
Zea mays/microbiologia
Zea mays/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170612
[Lr] Data última revisão:
170612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b05764


  7 / 6083 MEDLINE  
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[PMID]:28346361
[Au] Autor:Andrews M; Andrews ME
[Ad] Endereço:Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln 7647, New Zealand. mitchell.andrews@lincoln.ac.nz.
[Ti] Título:Specificity in Legume-Rhizobia Symbioses.
[So] Source:Int J Mol Sci;18(4), 2017 Mar 26.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Most species in the Leguminosae (legume family) can fix atmospheric nitrogen (N2) via symbiotic bacteria (rhizobia) in root nodules. Here, the literature on legume-rhizobia symbioses in field soils was reviewed and genotypically characterised rhizobia related to the taxonomy of the legumes from which they were isolated. The Leguminosae was divided into three sub-families, the Caesalpinioideae, Mimosoideae and Papilionoideae. spp. were the exclusive rhizobial symbionts of species in the Caesalpinioideae, but data are limited. Generally, a range of rhizobia genera nodulated legume species across the two Mimosoideae tribes Ingeae and Mimoseae, but spp. show specificity towards in central and southern Brazil, / in central Mexico and in southern Uruguay. These specific symbioses are likely to be at least in part related to the relative occurrence of the potential symbionts in soils of the different regions. Generally, Papilionoideae species were promiscuous in relation to rhizobial symbionts, but specificity for rhizobial genus appears to hold at the tribe level for the Fabeae ( ), the genus level for ( ), ( ) and the New Zealand native spp. ( ) and species level for ( ), ( ) and ( ). Specificity for rhizobial species/symbiovar appears to hold for ( sv. ) ( sv. ), ( ), ( sv. ), ( sv. s) and ( sv. ). Lateral gene transfer of specific symbiosis genes within rhizobial genera is an important mechanism allowing legumes to form symbioses with rhizobia adapted to particular soils. Strain-specific legume rhizobia symbioses can develop in particular habitats.
[Mh] Termos MeSH primário: Fabaceae/microbiologia
Rhizobium/fisiologia
Simbiose
[Mh] Termos MeSH secundário: Proteínas de Bactérias/classificação
Proteínas de Bactérias/genética
Bradyrhizobium/classificação
Bradyrhizobium/genética
Bradyrhizobium/fisiologia
Cupriavidus/classificação
Cupriavidus/fisiologia
Fabaceae/metabolismo
Filogenia
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
Rhizobium/classificação
Rhizobium/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170507
[Lr] Data última revisão:
170507
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


  8 / 6083 MEDLINE  
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[PMID]:28334155
[Au] Autor:Dall'Agnol RF; Bournaud C; de Faria SM; Béna G; Moulin L; Hungria M
[Ad] Endereço:Soil Biotechnology Laboratory, Embrapa Soja, C.P. 231, 86001-970, Londrina, PR, Brazil.
[Ti] Título:Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlântica).
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called ß-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny. Most strains were assembled in the genus Paraburkholderia, including Paraburkholderia sabiae and Pa. nodosa. Mesorhizobium (α-rhizobia) and Cupriavidus (ß-rhizobia) were also isolated, but in smaller proportions. Multilocus sequence analysis and BOX-PCR analyses indicated that six clusters of Paraburkholderia represent potential new species. In the phylogenetic analysis of the nodC gene, the majority of the strains were positioned in the same groups as in the 16S rRNA-recA tree, indicative of stability and vertical inheritance, but we also identified horizontal transfer of nodC in Pa. sabiae. All α- and ß-rhizobial species were trapped by both legumes, although preferences of the host plants for specific rhizobial species have been observed.
[Mh] Termos MeSH primário: Betaproteobacteria/genética
Florestas
Variação Genética
Mimosa/microbiologia
Phaseolus/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Brasil
Cupriavidus/classificação
RNA Ribossômico 16S/genética
Rhizobium/genética
Solo
Microbiologia do Solo
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Soil)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix027


  9 / 6083 MEDLINE  
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[PMID]:28284052
[Au] Autor:Chambon R; Pradeau S; Fort S; Cottaz S; Armand S
[Ad] Endereço:Univ. Grenoble Alpes, CERMAV, F-38000 Grenoble, France; CNRS, CERMAV, F-38000 Grenoble, France.
[Ti] Título:High yield production of Rhizobium NodB chitin deacetylase and its use for in vitro synthesis of lipo-chitinoligosaccharide precursors.
[So] Source:Carbohydr Res;442:25-30, 2017 Apr 10.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lipo-chitinoligosaccharides (LCOs) are key molecules for the establishment of plant-microorganisms symbiosis. Interactions of leguminous crops with nitrogen-fixing rhizobial bacteria involve Nod factors, while Myc-LCOs improve the association of most plants with arbuscular mycorrhizal fungi. Both Nod factors and Myc-LCOs are composed of a chitinoligosaccharide fatty acylated at the non-reducing end accompanied with various substituting groups. One straightforward way to access LCOs is starting from chitin hydrolysate, an abundant polysaccharide found in crustacean shells, followed by regioselective enzymatic cleavage of an acetyl group from the non-reducing end of chitin tetra- or pentaose, and subsequent chemical introduction of N-acyl group. In the present work, we describe the in vitro synthesis of LCO precursors on preparative scale. To this end, Sinorhizobium meliloti chitin deacetylase NodB was produced in high yield in E. coli as a thioredoxin fusion protein. The recombinant enzyme was expressed in soluble and catalytically active form and used as an efficient biocatalyst for N-deacetylation of chitin tetra- and pentaose.
[Mh] Termos MeSH primário: Amidoidrolases/biossíntese
Amidoidrolases/metabolismo
Lipopolissacarídeos/biossíntese
Rhizobium/metabolismo
[Mh] Termos MeSH secundário: Amidoidrolases/isolamento & purificação
Lipopolissacarídeos/química
Estrutura Molecular
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Rhizobium/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Recombinant Proteins); 0 (lipid-linked oligosaccharides); EC 3.5.- (Amidohydrolases); EC 3.5.1.41 (chitin deacetylase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE


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[PMID]:28277074
[Au] Autor:Madariaga-Navarrete A; Rodríguez-Pastrana BR; Villagómez-Ibarra JR; Acevedo-Sandoval OA; Perry G; Islas-Pelcastre M
[Ad] Endereço:a Institute of Agricultural Sciences, Agronomy and Forestry Area, Universidad Autónoma del Estado de Hidalgo , Tulancingo , Hidalgo , Mexico.
[Ti] Título:Bioremediation model for atrazine contaminated agricultural soils using phytoremediation (using Phaseolus vulgaris L.) and a locally adapted microbial consortium.
[So] Source:J Environ Sci Health B;52(6):367-375, 2017 Jun 03.
[Is] ISSN:1532-4109
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of the present study was to examine a biological model under greenhouse conditions for the bioremediation of atrazine contaminated soils. The model consisted in a combination of phytoremediation (using Phaseolus vulgaris L.) and rhizopheric bio-augmentation using native Trichoderma sp., and Rhizobium sp. microorganisms that showed no inhibitory growth at 10,000 mg L of herbicide concentration. 33.3 mg of atrazine 50 g of soil of initial concentration was used and an initial inoculation of 1 × 10 UFC mL of Rhizobium sp. and 1 × 10 conidia mL of Trichoderma sp. were set. Four treatments were arranged: Bean + Trichoderma sp. (B+T); Bean + Rhizobium sp. (BR); Bean + Rhizobium sp. + Trichoderma sp. (B+R+T) and Bean (B). 25.51 mg of atrazine 50 g of soil (76.63%) was removed by the B+T treatment in 40 days (a = 0.050, Tukey). This last indicate that the proposed biological model and methodology developed is useful for atrazine contaminated bioremediation agricultural soils, which can contribute to reduce the effects of agrochemical abuse.
[Mh] Termos MeSH primário: Atrazina/metabolismo
Biodegradação Ambiental
Consórcios Microbianos
Phaseolus/fisiologia
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Agricultura
Atrazina/análise
Herbicidas/análise
Herbicidas/metabolismo
México
Modelos Biológicos
Phaseolus/efeitos dos fármacos
Rhizobium/efeitos dos fármacos
Rhizobium/metabolismo
Rizosfera
Microbiologia do Solo
Poluentes do Solo/análise
Trichoderma/efeitos dos fármacos
Trichoderma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Soil Pollutants); QJA9M5H4IM (Atrazine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/03601234.2017.1292092



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