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[PMID]:28641560
[Au] Autor:Georgakis N; Chronopoulou E; Gad MA; Skliros D; Efrose R; Flemetakis E; Labrou NE
[Ad] Endereço:Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos Street, GR-11855-Athens. Greece.
[Ti] Título:Functional and Catalytic Characterization of the Detoxifying Enzyme Haloalkane Dehalogenase from Rhizobium leguminosarum.
[So] Source:Protein Pept Lett;24(7):599-608, 2017.
[Is] ISSN:1875-5305
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Haloalkane dehalogenases (EC 3.8.1.5, HLDs) are α/ß-hydrolases which catalyze the irreversible cleavage of carbon-halogen bonds of haloalkanes, producing an alcohol, a halide and a hydrogen ion. Haloalkanes are acutely toxic to animals and humans and their toxic effects are mainly observed in the liver, kidneys and central nervous system. OBJECTIVE: In the present work, the haloalkane dehalogenase from Rhizobium leguminosarum bv. trifolii (DrlA) was characterized. METHOD: Reverse transcription polymerase chain reaction analysis and enzyme activity assays revealed that the DrlA gene expression in R. leguminosarum bv. trifolii is induced by 1,2- dibromoethane (1,2-DBE) during the early exponential phase. The gene of the enzyme was isolated, cloned and expressed in E. coli Rosetta (DE3). RESULTS: Recombinant DrlA displays its high catalytic activity towards 1,2-DBE and the long-chain haloalkane 1-iodohexane. Limited activity was observed for other aliphatic and cyclic haloalkanes, indicating that the enzyme displays restricted substrate specificity, compared to other bacterial HLDs. Homology modelling and phylogenetic analysis suggested that the enzyme belongs to the HLD-II subfamily and shares the same overall fold and domain organization as other bacterial HLDs, however major variations were identified at the hydrophobic substrate-binding cavity, the cap domain and the entrance of the main tunnel that affect the size of the active site pocket and the substrate recognition mechanism. CONCLUSION: This work sheds new light on the environmental fate and toxicity of 1,2-DBE and provides new knowledge on the structure, function and diversity of HLDs for developing applications in toxicology.
[Mh] Termos MeSH primário: Catálise
Hidrolases/metabolismo
Rhizobium leguminosarum/enzimologia
[Mh] Termos MeSH secundário: Sítios de Ligação
Domínio Catalítico
Cristalografia por Raios X
Recuperação e Remediação Ambiental
Escherichia coli/genética
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Hidrocarbonetos Bromados/química
Hidrocarbonetos Bromados/toxicidade
Hidrolases/química
Hidrolases/genética
Modelos Moleculares
Filogenia
Dobramento de Proteína
Rhizobium leguminosarum/química
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons, Brominated); EC 3.- (Hydrolases); EC 3.8.1.5 (haloalkane dehalogenase); LI8384T9PH (bromoethane)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.2174/0929866524666170621094531


  2 / 705 MEDLINE  
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[PMID]:27795326
[Au] Autor:Wheatley RM; Ramachandran VK; Geddes BA; Perry BJ; Yost CK; Poole PS
[Ad] Endereço:Department of Plant Sciences, University of Oxford, Oxford, United Kingdom.
[Ti] Título:Role of O2 in the Growth of Rhizobium leguminosarum bv. viciae 3841 on Glucose and Succinate.
[So] Source:J Bacteriol;199(1), 2017 Jan 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insertion sequencing (INSeq) analysis of Rhizobium leguminosarum bv. viciae 3841 (Rlv3841) grown on glucose or succinate at both 21% and 1% O was used to understand how O concentration alters metabolism. Two transcriptional regulators were required for growth on glucose (pRL120207 [eryD] and RL0547 [phoB]), five were required on succinate (pRL100388, RL1641, RL1642, RL3427, and RL4524 [ecfL]), and three were required on 1% O (pRL110072, RL0545 [phoU], and RL4042). A novel toxin-antitoxin system was identified that could be important for generation of new plasmidless rhizobial strains. Rlv3841 appears to use the methylglyoxal pathway alongside the Entner-Doudoroff (ED) pathway and tricarboxylic acid (TCA) cycle for optimal growth on glucose. Surprisingly, the ED pathway was required for growth on succinate, suggesting that sugars made by gluconeogenesis must undergo recycling. Altered amino acid metabolism was specifically needed for growth on glucose, including RL2082 (gatB) and pRL120419 (opaA, encoding omega-amino acid:pyruvate transaminase). Growth on succinate specifically required enzymes of nucleobase synthesis, including ribose-phosphate pyrophosphokinase (RL3468 [prs]) and a cytosine deaminase (pRL90208 [codA]). Succinate growth was particularly dependent on cell surface factors, including the PrsD-PrsE type I secretion system and UDP-galactose production. Only RL2393 (glnB, encoding nitrogen regulatory protein PII) was specifically essential for growth on succinate at 1% O , conditions similar to those experienced by N -fixing bacteroids. Glutamate synthesis is constitutively activated in glnB mutants, suggesting that consumption of 2-ketoglutarate may increase flux through the TCA cycle, leading to excess reductant that cannot be reoxidized at 1% O and cell death. IMPORTANCE: Rhizobium leguminosarum, a soil bacterium that forms N -fixing symbioses with several agriculturally important leguminous plants (including pea, vetch, and lentil), has been widely utilized as a model to study Rhizobium-legume symbioses. Insertion sequencing (INSeq) has been used to identify factors needed for its growth on different carbon sources and O levels. Identification of these factors is fundamental to a better understanding of the cell physiology and core metabolism of this bacterium, which adapts to a variety of different carbon sources and O tensions during growth in soil and N fixation in symbiosis with legumes.
[Mh] Termos MeSH primário: Glucose/metabolismo
Oxigênio/farmacologia
Rhizobium leguminosarum/crescimento & desenvolvimento
Ácido Succínico/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Meios de Cultura
Relação Dose-Resposta a Droga
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Regulação Bacteriana da Expressão Gênica/fisiologia
Técnicas de Amplificação de Ácido Nucleico
Oxigênio/administração & dosagem
Oxigênio/metabolismo
Rhizobium leguminosarum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); AB6MNQ6J6L (Succinic Acid); IY9XDZ35W2 (Glucose); S88TT14065 (Oxygen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


  3 / 705 MEDLINE  
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[PMID]:27814683
[Au] Autor:Kishi LT; Fernandes CC; Omori WP; Campanharo JC; Lemos EG
[Ad] Endereço:Departamento de Tecnologia, Laboratório de Bioquímica de Microrganismos e Planta - LBMP, UNESP - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil.
[Ti] Título:Reclassification of the taxonomic status of SEMIA3007 isolated in Mexico B-11A Mex as Rhizobium leguminosarum bv. viceae by bioinformatic tools.
[So] Source:BMC Microbiol;16(1):260, 2016 Nov 04.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Evidence based on genomic sequences is extremely important to confirm the phylogenetic relationships within the Rhizobium group. SEMIA3007 was analyzed within the Mesorhizobium groups to define the underlying causes of taxonomic identification. We previously used biochemical tests and phenotypic taxonomic methods to identify bacteria, which can lead to erroneous classification. An improved understanding of bacterial strains such as the Mesorhizobium genus would increase our knowledge of classification and evolution of these species. RESULTS: In this study, we sequenced the complete genome of SEMIA3007 and compared it with five other Mesorhizobium and two Rhizobium genomes. The genomes of isolated SEMIA3007 showed several orthologs with M. huakuii, M. erdmanii and M. loti. We identified SEMIA3007 as a Mesorhizobium by comparing the 16S rRNA gene and the complete genome. CONCLUSION: Our ortholog, 16S rRNA gene and average nucleotide identity values (ANI) analysis all demonstrate SEMIA3007 is not Rhizobium leguminosarum bv. viceae. The results of the phylogenetic analysis clearly show SEMIA3007 is part of the Mesorhizobium group and suggest a reclassification is warranted.
[Mh] Termos MeSH primário: Biologia Computacional
Filogenia
Rhizobium leguminosarum/classificação
Rhizobium leguminosarum/genética
Rhizobium leguminosarum/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Bases
Classificação
DNA Bacteriano/genética
DNA Ribossômico/genética
Genoma Bacteriano
Mesorhizobium/classificação
Mesorhizobium/genética
México
Anotação de Sequência Molecular
RNA Ribossômico 16S/genética
Rhizobium/classificação
Rhizobium/genética
Rhizobium leguminosarum/crescimento & desenvolvimento
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


  4 / 705 MEDLINE  
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[PMID]:27760230
[Au] Autor:Ciesla J; Kopycinska M; Lukowska M; Bieganowski A; Janczarek M
[Ad] Endereço:Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290, Lublin, Poland.
[Ti] Título:Surface Properties of Wild-Type Rhizobium leguminosarum bv. trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
[So] Source:PLoS One;11(10):e0165080, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rhizobium leguminosarum bv. trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.). Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces. Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions. In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv. trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH). We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface. From the tested strains, the electrophoretic mobility was lowest in EPS-deficient pssA mutant. Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface. This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction. Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
[Mh] Termos MeSH primário: Polissacarídeos Bacterianos/genética
Polissacarídeos Bacterianos/metabolismo
Rhizobium leguminosarum/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Meios de Cultura/química
Glicosiltransferases/genética
Glicosiltransferases/metabolismo
Concentração de Íons de Hidrogênio
Rhizobium leguminosarum/metabolismo
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 0 (Polysaccharides, Bacterial); EC 2.4.- (Glycosyltransferases); EC 2.4.1.- (PssA protein, Rhizobium leguminosarum)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0165080


  5 / 705 MEDLINE  
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[PMID]:27501983
[Au] Autor:Terpolilli JJ; Masakapalli SK; Karunakaran R; Webb IU; Green R; Watmough NJ; Kruger NJ; Ratcliffe RG; Poole PS
[Ad] Endereço:Centre for Rhizobium Studies, Murdoch University, Perth, Australia Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom.
[Ti] Título:Lipogenesis and Redox Balance in Nitrogen-Fixing Pea Bacteroids.
[So] Source:J Bacteriol;198(20):2864-75, 2016 Oct 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Within legume root nodules, rhizobia differentiate into bacteroids that oxidize host-derived dicarboxylic acids, which is assumed to occur via the tricarboxylic acid (TCA) cycle to generate NAD(P)H for reduction of N2 Metabolic flux analysis of laboratory-grown Rhizobium leguminosarum showed that the flux from [(13)C]succinate was consistent with respiration of an obligate aerobe growing on a TCA cycle intermediate as the sole carbon source. However, the instability of fragile pea bacteroids prevented their steady-state labeling under N2-fixing conditions. Therefore, comparative metabolomic profiling was used to compare free-living R. leguminosarum with pea bacteroids. While the TCA cycle was shown to be essential for maximal rates of N2 fixation, levels of pyruvate (5.5-fold reduced), acetyl coenzyme A (acetyl-CoA; 50-fold reduced), free coenzyme A (33-fold reduced), and citrate (4.5-fold reduced) were much lower in bacteroids. Instead of completely oxidizing acetyl-CoA, pea bacteroids channel it into both lipid and the lipid-like polymer poly-ß-hydroxybutyrate (PHB), the latter via a type III PHB synthase that is active only in bacteroids. Lipogenesis may be a fundamental requirement of the redox poise of electron donation to N2 in all legume nodules. Direct reduction by NAD(P)H of the likely electron donors for nitrogenase, such as ferredoxin, is inconsistent with their redox potentials. Instead, bacteroids must balance the production of NAD(P)H from oxidation of acetyl-CoA in the TCA cycle with its storage in PHB and lipids. IMPORTANCE: Biological nitrogen fixation by symbiotic bacteria (rhizobia) in legume root nodules is an energy-expensive process. Within legume root nodules, rhizobia differentiate into bacteroids that oxidize host-derived dicarboxylic acids, which is assumed to occur via the TCA cycle to generate NAD(P)H for reduction of N2 However, direct reduction of the likely electron donors for nitrogenase, such as ferredoxin, is inconsistent with their redox potentials. Instead, bacteroids must balance oxidation of plant-derived dicarboxylates in the TCA cycle with lipid synthesis. Pea bacteroids channel acetyl-CoA into both lipid and the lipid-like polymer poly-ß-hydroxybutyrate, the latter via a type II PHB synthase. Lipogenesis is likely to be a fundamental requirement of the redox poise of electron donation to N2 in all legume nodules.
[Mh] Termos MeSH primário: Lipogênese
Nitrogênio/metabolismo
Ervilhas/microbiologia
Rhizobium leguminosarum/metabolismo
[Mh] Termos MeSH secundário: Acetilcoenzima A/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Carbono/metabolismo
Ciclo do Ácido Cítrico
Hidroxibutiratos/metabolismo
Oxirredução
Ervilhas/fisiologia
Poliésteres/metabolismo
Ácido Pirúvico/metabolismo
Rhizobium leguminosarum/genética
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Hydroxybutyrates); 0 (Polyesters); 26063-00-3 (poly-beta-hydroxybutyrate); 72-89-9 (Acetyl Coenzyme A); 7440-44-0 (Carbon); 8558G7RUTR (Pyruvic Acid); N762921K75 (Nitrogen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160810
[St] Status:MEDLINE
[do] DOI:10.1128/JB.00451-16


  6 / 705 MEDLINE  
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[PMID]:27480612
[Au] Autor:Koper P; Zebracki K; Marczak M; Skorupska A; Mazur A
[Ad] Endereço:Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland.
[Ti] Título:RepB proteins of the multipartite Rhizobium leguminosarum bv. trifolii genome discriminate between centromere-like parS sequences for plasmid segregational stability.
[So] Source:Mol Microbiol;102(3):446-466, 2016 Nov.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The plasmids of the Rhizobiaceae family members and other Alphaproteobacteria are usually large, low copy-number and contain all elements necessary for active segregation and replication located in one operon comprising repABC genes. The genome of Rhizobium leguminosarum bv. trifolii TA1 (RtTA1) consists of a chromosome and four plasmids (pRleTA1a-d) with repABC operons. In this work, centromere-binding RepB proteins of four RtTA1 plasmids were studied. Stability assays of the truncated derivatives of repABC cassettes demonstrated that RepA, RepB proteins and parS-like elements constituted plasmid partitioning systems, while RepC were sufficient for their replication. Individual RepB proteins bound specifically to centromere-like parS elements of the parental plasmids, which was crucial step toward the proper segregation of plasmids into daughter cells. RtTA1 RepB proteins formed dimers and oligomers in the solution. The C-terminal part of RepB was responsible for dimerization, while the domain engaged in parS binding was located in the middle of the protein. It was concluded that the specific interaction between individual RepB proteins and their target sequences together with the substantial diversity of the Rep proteins and parS originating from different plasmids strongly contributed to the coexistence of several plasmids equipped with similar repABC cassettes in the multipartite bacterial genome.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Plasmídeos/metabolismo
Rhizobium leguminosarum/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Sequência de Bases
Centrômero
Replicação do DNA
DNA Bacteriano/genética
Proteínas de Ligação a DNA/genética
Genes Bacterianos
Genoma Bacteriano
Óperon
Plasmídeos/genética
Replicon/genética
Proteínas Repressoras
Rhizobium leguminosarum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA-Binding Proteins); 0 (RepB protein, Bacteria); 0 (Repressor Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160803
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13472


  7 / 705 MEDLINE  
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[PMID]:27394068
[Au] Autor:Sbabou L; Regragui A; Filali-Maltouf A; Ater M; Béna G
[Ad] Endereço:Laboratoire de Microbiologie et de Biologie Moléculaire, Faculté des Sciences, Université Mohammed V, Agdal, Rabat, Morocco. Electronic address: lailasbabou@gmail.com.
[Ti] Título:Local genetic structure and worldwide phylogenetic position of symbiotic Rhizobium leguminosarum strains associated with a traditional cultivated crop, Vicia ervilia, from Northern Morocco.
[So] Source:Syst Appl Microbiol;39(6):409-17, 2016 Sep.
[Is] ISSN:1618-0984
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A total of 212 symbiotic bacteria were isolated from nodules of Vicia ervilia, a traditional crop cultivated in Northern Morocco. The isolates were recovered from 10 different sites, trapped each time with the local cultivar grown in the same field. Four loci were sequenced in order to characterize the isolates, including two housekeeping genes (recA and glnII), one plasmidic symbiotic gene (nodC) and one locus from another plasmid (prL11). In several isolates, two different copies of glnII were detected and sequenced, suggesting a unique duplication event, which has never been reported previously. There was no correlation between the genetic differentiation among cultivars and among bacteria, showing that the evolution of the bacterial population was independent, at least partially, from the host plant. By placing the haplotypes in a wide-ranging phylogenetic reconstruction, it was shown that the diversity detected in Morocco was spread throughout the different clades detected worldwide. The differentiation between areas relied on frequency variations of haplotypes rather than a presence/absence pattern. This finding raises new questions concerning bacterial genetic resource preservation, and confirms the old tenet "everything is everywhere but the environment selects".
[Mh] Termos MeSH primário: Técnicas de Tipagem Bacteriana
Rhizobium leguminosarum/classificação
Rhizobium leguminosarum/genética
Nódulos Radiculares de Plantas/microbiologia
Vicia/microbiologia
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Bacteriano/genética
Genes Essenciais/genética
Variação Genética/genética
Marrocos
Filogenia
RNA Ribossômico 16S/genética
Recombinases Rec A/genética
Rhizobium leguminosarum/isolamento & purificação
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); EC 2.7.7.- (Rec A Recombinases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160711
[St] Status:MEDLINE


  8 / 705 MEDLINE  
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[PMID]:27269381
[Au] Autor:Van Cauwenberghe J; Lemaire B; Stefan A; Efrose R; Michiels J; Honnay O
[Ad] Endereço:Plant Conservation and Population Biology, Biology Department, KU Leuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium; Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium. Electronic address: jannick.vancauwenberghe@bio.kuleuven.be.
[Ti] Título:Symbiont abundance is more important than pre-infection partner choice in a Rhizobium - legume mutualism.
[So] Source:Syst Appl Microbiol;39(5):345-9, 2016 Jul.
[Is] ISSN:1618-0984
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:It is known that the genetic diversity of conspecific rhizobia present in root nodules differs greatly among populations of a legume species, which has led to the suggestion that both dispersal limitation and the local environment affect rhizobial genotypic composition. However, it remains unclear whether rhizobial genotypes residing in root nodules are representative of the entire population of compatible symbiotic rhizobia. Since symbiotic preferences differ among legume populations, the genetic composition of rhizobia found within nodules may reflect the preferences of the local hosts, rather than the full diversity of potential nodulating rhizobia present in the soil. Here, we assessed whether Vicia cracca legume hosts of different provenances select different Rhizobium leguminosarum genotypes than sympatric V. cracca hosts, when presented a natural soil rhizobial population. Through combining V. cracca plants and rhizobia from adjacent and more distant populations, we found that V. cracca hosts are relatively randomly associated with rhizobial genotypes. This indicates that pre-infection partner choice is relatively weak in certain legume hosts when faced with a natural population of rhizobia.
[Mh] Termos MeSH primário: Rhizobium leguminosarum/crescimento & desenvolvimento
Rhizobium leguminosarum/genética
Nódulos Radiculares de Plantas/microbiologia
Vicia/microbiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Sequência de Bases
Variação Genética/genética
Genótipo
Geografia
N-Acetilglucosaminiltransferases/genética
Recombinases Rec A/genética
Análise de Sequência de DNA
Solo/química
Microbiologia do Solo
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Soil); EC 2.4.1.- (N-Acetylglucosaminyltransferases); EC 2.4.1.- (NodC protein, Rhizobiales); EC 2.7.7.- (Rec A Recombinases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE


  9 / 705 MEDLINE  
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[PMID]:27267929
[Au] Autor:Sami D; Mokhtar R; Peter M; Mohamed M
[Ad] Endereço:Research Unit Biodiversity & Valorization of Arid Areas Bioressources, Faculty of Sciences of Gabès, Erriadh-Zrig, Gabes 6072, Tunisia.
[Ti] Título:Rhizobium leguminosarum symbiovar trifolii, Ensifer numidicus and Mesorhizobium amorphae symbiovar ciceri (or Mesorhizobium loti) are new endosymbiotic bacteria of Lens culinaris Medik.
[So] Source:FEMS Microbiol Ecol;92(8), 2016 08.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A total of 142 rhizobial bacteria were isolated from root nodules of Lens culinaris Medik endemic to Tunisia and they belonged to the species Rhizobium leguminosarum, and for the first time to Ensifer and Mesorhizobium, genera never previously described as microsymbionts of lentil. Phenotypically, our results indicate that L. culinaris Medik strains showed heterogenic responses to the different phenotypic features and they effectively nodulated their original host. Based on the concatenation of the 16S rRNA with relevant housekeeping genes (glnA, recA, dnaK), rhizobia that nodulate lentil belonged almost exclusively to the known R. leguminosarum sv. viciae. Interestingly, R. leguminosarum sv. trifolii, Ensifer numidicus (10 isolates) and Mesorhizobium amorphae (or M. loti) (9 isolates) isolates species, not considered, up to now, as a natural symbiont of lentil are reported. The E. numidicus and M. amorphae (or M. loti) strains induced fixing nodules on Medicago sativa and Cicer arietinum host plants, respectively. Symbiotic gene phylogenies showed that the E. numidicus, new symbiont of lentil, markedly diverged from strains of R. leguminosarum, the usual symbionts of lentil, and converged to the symbiovar meliloti so far described within E. meliloti Indeed, the nodC and nodA genes from the M. amorphae showed more than 99% similarity with respect to those from M. mediterraneum, the common chickpea nodulating species, and would be included in the new infrasubspecific division named M. amorphae symbiovar ciceri, or to M. loti, related to the strains able to effectively nodulate C. arietinum host plant. On the basis of these data, R. leguminosarum sv. trifolii (type strain LBg3 (T)), M. loti or M. amorphae sv. ciceri (type strain LB4 (T)) and E. numidicus (type strain LBi2 (T)) are proposed as new symbionts of L. culinaris Medik.
[Mh] Termos MeSH primário: Lens (Planta)/microbiologia
Mesorhizobium/isolamento & purificação
Rhizobiaceae/isolamento & purificação
Rhizobium leguminosarum/isolamento & purificação
Nódulos Radiculares de Plantas/microbiologia
[Mh] Termos MeSH secundário: Sequência de Bases
Cicer/microbiologia
DNA Bacteriano/genética
Genes Essenciais/genética
Mesorhizobium/classificação
Mesorhizobium/genética
Filogenia
RNA Ribossômico 16S/genética
Rhizobiaceae/classificação
Rhizobiaceae/genética
Rhizobium leguminosarum/classificação
Rhizobium leguminosarum/genética
Análise de Sequência de DNA
Simbiose/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE


  10 / 705 MEDLINE  
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[PMID]:27266251
[Au] Autor:Makarova LE; Dudareva LV; Petrova IG; Vasil'eva GG
[Ti] Título:[Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. Pisi].
[So] Source:Prikl Biokhim Mikrobiol;52(2):217-22, 2016 Mar-Apr.
[Is] ISSN:0555-1099
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The content of apigenin, naringenin, pisatin, dibutyl-ortho-phthalate, and N-phenyl-2-naphthyl-amine were assayed in root exudates of pea (Pisum sativum L.) seedlings one day after their inoculation with Rhizobium leguminosarum, bv. viceae or Pseudomonas siringae pv. pisi, which represent, respectively, mutualistic and antagonistic strategies of interaction with a host plant. After inoculation with either bacteria, the concentrations of apigenin and pisatin in the root exudates were equal, whereas the concentrations of naringenin and N-phenyl-2-naphthylamine were different and those of dibutyl-o-phthalate were unchanged. A certain role is suggested for the phenolic compounds in an accomplishment of symbiotic relations of bacteria with a host plant.
[Mh] Termos MeSH primário: Ervilhas/metabolismo
Fenóis/metabolismo
Raízes de Plantas/metabolismo
Simbiose
[Mh] Termos MeSH secundário: Ervilhas/microbiologia
Raízes de Plantas/microbiologia
Pseudomonas/metabolismo
Rhizobium leguminosarum/metabolismo
Plântulas/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenols)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160608
[Lr] Data última revisão:
160608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE



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