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  1 / 178 MEDLINE  
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[PMID]:28820086
[Au] Autor:Sánchez-Castro I; Ruiz-Fresneda MA; Bakkali M; Kämpfer P; Glaeser SP; Busse HJ; López-Fernández M; Martínez-Rodríguez P; Merroun ML
[Ad] Endereço:1​Departamento de Microbiología, Campus de Fuentenueva, Universidad de Granada, 18071 Granada, Spain.
[Ti] Título:Stenotrophomonas bentonitica sp. nov., isolated from bentonite formations.
[So] Source:Int J Syst Evol Microbiol;67(8):2779-2786, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas rhizophila e-p10T (99.2 % sequence similarity), followed by Stenotrophomonas pavanii ICB 89T (98.5 %), Stenotrophomonas maltophilia IAM 12423T, Stenotrophomonas chelatiphaga LPM-5T and Stenotrophomonas tumulicola T5916-2-1bT (all 98.3 %). Pairwise sequence similarities to all other type strains of species of the genus Stenotrophomonas were below 98 %. Genome-based calculations (orthologous average nucleotide identity, original average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C15 : 0 and anteiso-C15 : 0 as major components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7T. Based on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7T can be differentiated clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp. nov. is proposed with BII-R7T as the type strain (=LMG 29893T=CECT 9180T=DSM 103927T).
[Mh] Termos MeSH primário: Bentonita
Stenotrophomonas/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espanha
Stenotrophomonas/genética
Stenotrophomonas/isolamento & purificação
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 1302-78-9 (Bentonite); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002016


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[PMID]:28629502
[Au] Autor:Ouattara AS; Le Mer J; Joseph M; Macarie H
[Ad] Endereço:1​Laboratoire de Microbiologie et de Biotechnologie Microbienne, Ecole Doctorale Sciences et Technologies, Université Ouaga 1 Pr Joseph KI ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso.
[Ti] Título:Transfer of Pseudomonas pictorum Gray and Thornton 1928 to genus Stenotrophomonas as Stenotrophomonas pictorum comb. nov., and emended description of the genus Stenotrophomonas.
[So] Source:Int J Syst Evol Microbiol;67(6):1894-1900, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A polyphasic taxonomic approach including analysis of phenotypic, physiological and genotypic characteristics, 16S rRNA gene sequence and DNA-DNA hybridization analysis was used to determine the most consistent affiliation of Pseudomonas pictorum. Pseudomonas pictorum ATCC 23328T exhibited phenotypic traits of members of the genus Stenotrophomonas including cellular fatty acid composition, quinone and limited range of substrates that could be used. Antibiotic susceptibility and physiological characteristics were determined. The DNA G+C content was 65.7 mol%. Phylogenetic analysis revealed that the type strains of Stenotrophomonas terrae, Stenotrophomonashumi, Stenotrophomonasnitritireducens and Stenotrophomonasacidaminiphila were the nearest relatives (16S rRNA gene sequence similarity of 98.0 to 98.8 %). All the other type strains of species of the genus Stenotrophomonas showed high 16S rRNA gene sequence similarities (96.8 to 97.2 %). DNA-DNA hybridizations revealed 31.0, 32.0, 43.3 and 43.6 % reassociation between Pseudomonas pictorum ATCC 23328T and the type strains of S. terrae, S. humi, S. nitritireducens and S. acidaminiphila, respectively. Our overall results indicate that Pseudomonas pictorum should be transferred to the genus Stenotrophomonas as a novel species of this genus, Stenotrophomonas pictorum comb. nov. Since the original description of the genus Stenotrophomonaswas made with only one species (Stenotrophomonasmaltophilia), an emendation of the genus description is proposed in order to match better with the characteristics of the eleven novel species assigned to this genus since then.
[Mh] Termos MeSH primário: Filogenia
Pseudomonas/classificação
Stenotrophomonas/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001880


  3 / 178 MEDLINE  
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[PMID]:28000121
[Au] Autor:Chandra R; Kumar V
[Ad] Endereço:Environmental Microbiology Laboratory, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31 M.G. Marg, Lucknow, Uttar Pradesh, 226001, India. rc_microitrc@yahoo.co.in.
[Ti] Título:Detection of Bacillus and Stenotrophomonas species growing in an organic acid and endocrine-disrupting chemical-rich environment of distillery spent wash and its phytotoxicity.
[So] Source:Environ Monit Assess;189(1):26, 2017 Jan.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sugarcane molasses-based distillery spent wash (DSW) is well known for its toxicity and complex mixture of various recalcitrant organic pollutants with acidic pH, but the chemical nature of these pollutants is unknown. This study revealed the presence of toxic organic acids (butanedioic acid bis(TMS)ester; 2-hydroxysocaproic acid; benzenepropanoic acid, α-[(TMS)oxy], TMS ester; vanillylpropionic acid, bis(TMS)), and other recalcitrant organic pollutants (2-furancarboxylic acid, 5-[[(TMS)oxy] methyl], TMS ester; benzoic acid 3-methoxy-4-[(TMS)oxy], TMS ester; and tricarballylic acid 3TMS), which are listed as endocrine-disrupting chemicals. In addition, several major heavy metals were detected, including Fe (163.947), Mn (4.556), Zn (2.487), and Ni (1.175 mg l ). Bacterial community analysis by restriction fragment length polymorphism revealed that Bacillus and Stenotrophomonas were dominant autochthonous bacterial communities belonging to the phylum Firmicutes and γ-Proteobacteria, respectively. The presence of Bacillus and Stenotrophomonas species in highly acidic environments indicated its broad range adaptation. These findings indicated that these autochthonous bacterial communities were pioneer taxa for in situ remediation of this hazardous waste during ecological succession. Further, phytotoxicity assay of DSW with Phaseolus mungo L. and Triticum aestivum revealed that T. aestivum was more sensitive than P. mungo L. in the seed germination test. The results of this study may be useful for monitoring and toxicity assessment of sugarcane molasses-based distillery waste at disposal sites.
[Mh] Termos MeSH primário: Bacillus/isolamento & purificação
Disruptores Endócrinos/análise
Compostos Orgânicos/análise
Stenotrophomonas/isolamento & purificação
[Mh] Termos MeSH secundário: Monitoramento Ambiental/métodos
Resíduos Industriais/análise
Metais Pesados/análise
Melaço
Eliminação de Resíduos Líquidos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Industrial Waste); 0 (Metals, Heavy); 0 (Organic Chemicals)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-016-5746-9


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[PMID]:27899336
[Au] Autor:Kim KR; Kang WR; Oh DK
[Ad] Endereço:Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, South Korea.
[Ti] Título:Complete genome sequence of Stenotrophomonas sp. KACC 91585, an efficient bacterium for unsaturated fatty acid hydration.
[So] Source:J Biotechnol;241:108-111, 2017 Jan 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hydroxy fatty acids (HFAs) such as 10-hydroxystearic acid (10-HSA) and 10-hydroxy-12(Z)-octadecenoic acid (10-HOD), which are similar to ricinoleic acid, are important starting materials and intermediates for the industrial manufacture of many commodities. Stenotrophomonas sp. KACC 91585, which was isolated from lake sediment, is an efficient bacterium for unsaturated fatty acid hydration that produces 10-HSA and 10-HOD from oleic acid and linoleic acid, respectively, with high conversion rates. The complete genome of this strain is 4,541,729bp with 63.83% GC content and devoid of plasmids. Sets of genes involved in the fatty acid biosynthesis and modification as well as modified lipids were identified in the genome, and these genes were concerned with HFA production. This genome sequence provides molecular information and elucidation for HFA production, and will be used as an efficient biocatalyst source for the biotechnological production of HFA.
[Mh] Termos MeSH primário: Ácidos Graxos Insaturados/metabolismo
Genoma Bacteriano/genética
Stenotrophomonas/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/análise
DNA Bacteriano/genética
Ácidos Graxos Insaturados/química
Análise de Sequência de DNA
Stenotrophomonas/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids, Unsaturated)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE


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[PMID]:27889889
[Au] Autor:Gargouri B; Contreras MD; Ammar S; Segura-Carretero A; Bouaziz M
[Ad] Endereço:Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP 1173, 3038, Sfax, Tunisia.
[Ti] Título:Biosurfactant production by the crude oil degrading Stenotrophomonas sp. B-2: chemical characterization, biological activities and environmental applications.
[So] Source:Environ Sci Pollut Res Int;24(4):3769-3779, 2017 Feb.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this work, biosurfactant-producing microorganisms were isolated from hydrocarbon-contaminated water collected from Tunisian oilfield. After enrichment and isolation, different bacterial strains were preliminary studied for their biosurfactant/bioemulsifier properties when using crude oil as the unique carbon source. In particular, the isolate strain B-2, a Gram-negative, rod-shaped bacterium, efficiently emulsified crude oil. The extracellular biosurfactant product from this strain presented an emulsification activity above 70% and a hydrophobicity of 71%. In addition, a diameter of 6 cm was observed in the oil displacement test. The characterization of B-2 strain using 16S rDNA sequencing enables us to find a high degree of similarity with various members of the genus Stenotrophomonas (with a percentage of similarity of 99%). The emulsification activity of Stenotrophomonas biosurfactant B-2 was maintained in a wide range of pH (2 to 6), temperature (4 to 55 °C), and salinity (0 to 50 g L ) conditions. It also enhanced the solubility of phenanthrene in water and could be used in the re-mobilization of hydrocarbon-contaminated environment. In addition, this biosurfactant exhibited antimicrobial and antioxidant properties. Infrared spectroscopy suggested potential lipidic and peptidic moieties, and mass spectrometry-based analyses showed that the biosurfactant contains mainly cyclic peptidic structures belonging to the class of diketopiperazines. Therefore, the B-2 strain is a promising biosurfactant-producing microorganism and its derived biosurfactant presents a wide range of industrial applications.
[Mh] Termos MeSH primário: Petróleo/metabolismo
Stenotrophomonas/metabolismo
Tensoativos/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Hidrocarbonetos/metabolismo
Oxirredução
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons); 0 (Petroleum); 0 (Surface-Active Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-8064-4


  6 / 178 MEDLINE  
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[PMID]:27745913
[Au] Autor:Gandolfi I; Canedoli C; Imperato V; Tagliaferri I; Gkorezis P; Vangronsveld J; Padoa Schioppa E; Papacchini M; Bestetti G; Franzetti A
[Ad] Endereço:Dept. of Earth and Environmental Sciences, University of Milano-Bicocca, Milan, Italy. Electronic address: isabella.gandolfi@unimib.it.
[Ti] Título:Diversity and hydrocarbon-degrading potential of epiphytic microbial communities on Platanus x acerifolia leaves in an urban area.
[So] Source:Environ Pollut;220(Pt A):650-658, 2017 Jan.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plants and their associated bacteria have been suggested to play a role in air pollution mitigation, especially in urban areas. Particularly, epiphytic bacteria might be able to degrade atmospheric hydrocarbons. However, phyllospheric bacterial communities are highly variable depending on several factors, e.g. tree species, leaf age and physiology, environmental conditions. In this work, bacterial communities hosted by urban Platanus x acerifolia leaves were taxonomically characterized using high throughput sequencing of 16S rRNA gene, and their temporal and spatial variability was assessed by comparing samples collected from different locations in the city of Milan (Italy) and in different months. The diversity of alkane hydroxylase (alkB) phylotypes harboured by phyllospheric bacteria associated to urban Platanus trees was also evaluated. Results revealed that temporal changes, which are related to seasonality, acted as a stronger driver both on Platanus phyllospheric community structure and on alkB phylotype diversity than sampling location. Biodiversity of bacterial communities decreased along the growing season, leading to a strong dominance by the genus Stenotrophomonas. On the contrary, diversity of hydrocarbon-degrading populations increased over the months, although it resulted lower than that reported for other habitats. It was therefore hypothesized that atmospheric hydrocarbons might play a key role in the selection of phyllospheric populations in urban areas.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/metabolismo
Hidrocarbonetos/metabolismo
Magnoliopsida/microbiologia
Folhas de Planta/microbiologia
Stenotrophomonas/classificação
Stenotrophomonas/metabolismo
Árvores/microbiologia
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Biodiversidade
Citocromo P-450 CYP4A/genética
Itália
Filogenia
RNA Ribossômico 16S/genética
Estações do Ano
Stenotrophomonas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Hydrocarbons); 0 (RNA, Ribosomal, 16S); EC 1.14.15.3 (Cytochrome P-450 CYP4A)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:27474883
[Au] Autor:Kang WR; Seo MJ; Shin KC; Park JB; Oh DK
[Ad] Endereço:Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.
[Ti] Título:Gene cloning of an efficiency oleate hydratase from Stenotrophomonas nitritireducens for polyunsaturated fatty acids and its application in the conversion of plant oils to 10-hydroxy fatty acids.
[So] Source:Biotechnol Bioeng;114(1):74-82, 2017 Jan.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hydroxy fatty acids are used as precursors of lactones and dicarboxylic acids, as starting materials of polymers, and as additives in coatings and paintings. Stenotrophomonas nitritireducens efficiently converts cis-9 polyunsaturated fatty acids (PUFAs) to 10-hydroxy fatty acids. However, gene encoding enzyme involved in this conversion has not been identified to date. We purified a putative fatty acid double-bond hydratase from S. nitritireducens by ultrafiltration and HiPrep DEAE FF and Resource Q ion exchange chromatographies. Peptide sequences of the purified enzyme were obtained by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis. Sequence of the partial gene encoding this putative fatty acid double-bond hydratase was determined by degenerate polymerase chain reaction (PCR) based on the peptide sequences. The remaining gene sequence was identified by rapid amplification of cDNA ends using cDNA of S. nitritireducens as a template, and the full-length gene was cloned subsequently. The expressed enzyme was identified as an oleate hydratase by determining its kinetic parameters toward unsaturated fatty acids. S. nitritireducens oleate hydratase showed higher activity toward PUFAs compared with other available oleate hydratases. This suggested that the enzyme could be used effectively to convert plant oils to 10-hydroxy fatty acids because these oils contained unsaturated fatty acids such as oleic acid (OA) and linoleic acid (LA) and PUFAs such as α-linolenic acid and/or γ-linolenic acid. The enzyme converted soybean oil and perilla seed oil hydrolyzates containing 10 mM total unsaturated fatty acids, including OA, LA, and ALA, to 8.87 and 8.70 mM total 10-hydroxy fatty acids, respectively, in 240 min. To our knowledge, this is the first study on the biotechnological conversion of PUFA-containing oils to hydroxy fatty acids. Biotechnol. Bioeng. 2017;114: 74-82. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Clonagem Molecular/métodos
Hidroliases/genética
Ácido Oleico/metabolismo
Proteínas Recombinantes/genética
Stenotrophomonas/enzimologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Escherichia coli/genética
Ácidos Graxos Insaturados/análise
Ácidos Graxos Insaturados/metabolismo
Hidroliases/química
Hidroliases/metabolismo
Ácido Oleico/química
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Stenotrophomonas/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fatty Acids, Unsaturated); 0 (Recombinant Proteins); 2UMI9U37CP (Oleic Acid); EC 4.2.1.- (Hydro-Lyases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26058


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[PMID]:27624084
[Au] Autor:Ortega RA; Mahnert A; Berg C; Müller H; Berg G
[Ad] Endereço:Institute of Environmental Biotechnology, Graz University of Technology, 8010 Graz, Austria University of the Philippines Baguio, 2600 Baguio City, Philippines.
[Ti] Título:The plant is crucial: specific composition and function of the phyllosphere microbiome of indoor ornamentals.
[So] Source:FEMS Microbiol Ecol;92(12), 2016 Dec.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The plant microbiome is a key determinant of plant health. Less is known about the phyllosphere microbiota and its driving factors in built environments. To study the variability of the microbiome in relation to plant genotype and climate under different controlled conditions, we investigated 14 phylogenetically diverse plant species grown in the greenhouses of the Botanical Garden in Graz (Austria). All investigated plants showed specific bacterial abundances of up to 10(6) CFU cm(-2) on their leaves. Bacterial diversity (H('): 2.4-7.9) and number of putative OTUs (461-2013) were strongly plant species dependent. Statistical analysis showed a significantly higher correlation of community composition to plant genotype in comparison to the ambient climatic variables. In addition to the microbiome structure, we studied the antagonistic potential towards the foliar pathogen Botrytis cinerea as functional indicator. A high proportion of isolates (up to 58%) were able to inhibit pathogen growth by production of volatile organic compounds (VOCs). Data of structure and function were linked: frequently isolated VOCs producers (e.g. Bacillus and Stenotrophomonas) were highly present in phyllosphere communities, which were dominated by members of Firmicutes This study indicates that indoor ornamentals feature a distinct, stable microbiota on leaves irrespective of the indoor climate.
[Mh] Termos MeSH primário: Botrytis/crescimento & desenvolvimento
Microbiota
Folhas de Planta/microbiologia
Plantas/microbiologia
Compostos Orgânicos Voláteis/metabolismo
[Mh] Termos MeSH secundário: Áustria
Bacillus/genética
Bacillus/isolamento & purificação
Bacillus/metabolismo
Biodiversidade
Firmicutes/isolamento & purificação
Filogenia
Plantas/genética
RNA Ribossômico 16S/genética
Stenotrophomonas/genética
Stenotrophomonas/isolamento & purificação
Stenotrophomonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160915
[St] Status:MEDLINE


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[PMID]:27451445
[Au] Autor:Wollenberg AC; Jagdish T; Slough G; Hoinville ME; Wollenberg MS
[Ad] Endereço:Biology Department, Kalamazoo College, Kalamazoo, Michigan, USA.
[Ti] Título:Death Becomes Them: Bacterial Community Dynamics and Stilbene Antibiotic Production in Cadavers of Galleria mellonella Killed by Heterorhabditis and Photorhabdus spp.
[So] Source:Appl Environ Microbiol;82(19):5824-37, 2016 Oct 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Insect larvae killed by entomopathogenic nematodes are thought to contain bacterial communities dominated by a single bacterial genus, that of the nematode's bacterial symbiont. In this study, we used next-generation sequencing to profile bacterial community dynamics in greater wax moth (Galleria mellonella) larvae cadavers killed by Heterorhabditis nematodes and their Photorhabdus symbionts. We found that, although Photorhabdus strains did initially displace an Enterococcus-dominated community present in uninfected G. mellonella insect larvae, the cadaver community was not static. Twelve days postinfection, Photorhabdus shared the cadaver with Stenotrophomonas species. Consistent with this result, Stenotrophomonas strains isolated from infected cadavers were resistant to Photorhabdus-mediated toxicity in solid coculture assays. We isolated and characterized a Photorhabdus-produced antibiotic from G. mellonella cadavers, produced it synthetically, and demonstrated that both the natural and synthetic compounds decreased G. mellonella-associated Enterococcus growth, but not Stenotrophomonas growth, in vitro Finally, we showed that the Stenotrophomonas strains described here negatively affected Photorhabdus growth in vitro Our results add an important dimension to a broader understanding of Heterorhabditis-Photorhabdus biology and also demonstrate that interspecific bacterial competition likely characterizes even a theoretically monoxenic environment, such as a Heterorhabditis-Photorhabdus-parasitized insect cadaver. IMPORTANCE: Understanding, and eventually manipulating, both human and environmental health depends on a complete accounting of the forces that act on and shape microbial communities. One of these underlying forces is hypothesized to be resource competition. A resource that has received little attention in the general microbiological literature, but likely has ecological and evolutionary importance, is dead/decaying multicellular organisms. Metazoan cadavers, including those of insects, are ephemeral and nutrient-rich environments, where resource competition might shape interspecific macrobiotic and microbiotic interactions. This study is the first to use a next-generation sequencing approach to study the community dynamics of bacteria within a model insect cadaver system: insect larvae parasitized by entomopathogenic nematodes and their bacterial symbionts. By integrating bioinformatic, biochemical, and classic in vitro microbiological approaches, we have provided mechanistic insight into how antibiotic-mediated bacterial interactions may shape community dynamics within insect cadavers.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Microbiota
Mariposas/microbiologia
Mariposas/parasitologia
Photorhabdus/fisiologia
Rabditídios/fisiologia
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/isolamento & purificação
Cadáver
Larva/crescimento & desenvolvimento
Larva/microbiologia
Larva/parasitologia
Microbiota/efeitos dos fármacos
Microbiota/genética
Mariposas/crescimento & desenvolvimento
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Stenotrophomonas/efeitos dos fármacos
Estilbenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Stilbenes)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01211-16


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[PMID]:27342606
[Au] Autor:Tiwari B; Manickam N; Kumari S; Tiwari A
[Ad] Endereço:Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad 211 007, India; Environmental Biotechnology Section, CSIR-Indian Institute of Toxicology Research, Lucknow 226 001, India.
[Ti] Título:Biodegradation and dissolution of polyaromatic hydrocarbons by Stenotrophomonas sp.
[So] Source:Bioresour Technol;216:1102-5, 2016 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to study the biodegradation capabilities of a locally isolated bacterium, Stenotrophomonas sp. strain IITR87 to degrade the polycyclic aromatic hydrocarbons and also check the preferential biodegradation of polycyclic aromatic hydrocarbons (PAHs). From preferential substrate degradation studies, it was found that Stenotrophomonas sp. strain IITR87 first utilized phenanthrene (three membered ring), followed by pyrene (four membered ring), then benzo[α]pyrene (five membered ring). Dissolution study of PAHs with surfactants, rhamnolipid and tritonX-100 showed that the dissolution of PAHs increased in the presence of surfactants.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Hidrocarbonetos Aromáticos Policíclicos
Stenotrophomonas
[Mh] Termos MeSH secundário: Hidrocarbonetos Aromáticos Policíclicos/análise
Hidrocarbonetos Aromáticos Policíclicos/química
Hidrocarbonetos Aromáticos Policíclicos/metabolismo
Solubilidade
Stenotrophomonas/química
Stenotrophomonas/metabolismo
Tensoativos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polycyclic Aromatic Hydrocarbons); 0 (Surface-Active Agents)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160626
[St] Status:MEDLINE



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