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[PMID]:22961460
[Au] Autor:Harder S; Podschun R; Grancicova L; Mehl C; Kern M
[Ad] Endereço:Department of Prosthodontics, Propaedeutics and Dental Materials, Christian-Albrechts University, Arnold-Heller Str. 16, Kiel, 24105, Germany. sharder@proth.uni-kiel.de
[Ti] Título:Analysis of the intraimplant microflora of two-piece dental implants.
[So] Source:Clin Oral Investig;17(4):1135-42, 2013 May.
[Is] ISSN:1436-3771
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Information about the spectrum of microorganisms in the intraimplant cavities of two-piece dental implants is scarce. The purpose of this study was to assess the intraimplant microflora of two-piece dental implants by conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16 s rDNA gene sequencing. MATERIALS AND METHODS: Ten patients (six men and four women; average age = 66.7 years; age range = 58-78 years) received 35 two-piece titanium implants carrying ball attachments. Biofilm sampling was performed with sterile microbrushes, and nonadherent microbial samples were obtained by injection and reuptake of predefined volumes of NaCl solution. The samples were cultured and analyzed by conventional biochemical testing, MALDI-TOF MS, and 16 s rDNA gene sequencing. RESULTS: Of the 103 species detected, 27 and 33 were identified only in the biofilm and nonadherent microbial samples, respectively. Forty-three species were identified in both types of samples. CONCLUSIONS: Two-piece dental implants harbored a broad spectrum of gram-positive and gram-negative aerobes and anaerobes, especially rods and cocci. CLINICAL RELEVANCE: These findings confirm bacterial translocation from the oral cavity to intraimplant cavities. Microbiological methods as used in this study are necessary to reveal the complete vital microflora of intraimplant cavities.
[Mh] Termos MeSH primário: Projeto do Implante Dentário-Pivô
Implantes Dentários/microbiologia
Microbiota
Alvéolo Dental/microbiologia
[Mh] Termos MeSH secundário: Idoso
Bactérias Aeróbias/genética
Bactérias Anaeróbias/genética
Biofilmes
Contagem de Colônia Microbiana
DNA Bacteriano/análise
DNA Bacteriano/genética
Feminino
Bacilos e Cocos Aeróbios Gram-Negativos/genética
Cocos Anaeróbios Gram-Negativos/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Cocos Gram-Positivos/genética
Bacilos Gram-Positivos/genética
Seres Humanos
Masculino
Meia-Idade
Análise de Sequência de DNA
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Dental Implants)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:120911
[St] Status:MEDLINE
[do] DOI:10.1007/s00784-012-0805-2


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[PMID]:19700920
[Au] Autor:Fukunaga Y; Kurahashi M; Sakiyama Y; Ohuchi M; Yokota A; Harayama S
[Ad] Endereço:NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), Chiba, Japan. fukunaga-yukiyo@nite.go.jp
[Ti] Título:Phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of Phycisphaeraceae fam. nov., Phycisphaerales ord. nov. and Phycisphaerae classis nov. in the phylum Planctomycetes.
[So] Source:J Gen Appl Microbiol;55(4):267-75, 2009 Aug.
[Is] ISSN:0022-1260
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Three strains, FYK2301M01(T), FYK2301M18 and FYK2301M52, all being Gram-negative, spherical, motile and facultatively anaerobic, were isolated from a marine alga (Porphyra sp.) collected on Mikura Island, Japan. Colonies of the strains were circular and pink-pigmented on Marine Agar 2216 (Difco) at 25 degrees C. Cells of the strains reproduced by binary fission. The G+C content of the DNA was 73 mol%. The major isoprenoid quinone was MK-6. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains are the members of the WPS-1 group (Nogales et al., 2001) comprising no validly described taxa within the phylum Planctomycetes. The highest similarity value of the 16S rRNA gene sequences of the strains to those in the established bacterial taxa was only 78.7% to Planctomyces brasiliensis DSM 5305(T). From the taxonomic data obtained in this study, it is proposed that the new marine isolates be placed in a novel genus and species named Phycisphaera mikurensis gen. nov., sp. nov. within a new family, order and class Phycisphaeraceae fam. nov., Phycisphaerales ord. nov. and Phycisphaerae classis nov. in the phylum Planctomycetes. The type strain of Phycisphaera mikurensis is FYK2301M01(T) (= NBRC 102666(T) = KCTC 22515(T)).
[Mh] Termos MeSH primário: Cocos Anaeróbios Gram-Negativos/classificação
Cocos Anaeróbios Gram-Negativos/isolamento & purificação
Porphyra/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Composição de Bases
DNA Bacteriano/análise
DNA Bacteriano/genética
Cocos Anaeróbios Gram-Negativos/genética
Cocos Anaeróbios Gram-Negativos/ultraestrutura
Japão
Microscopia Eletrônica de Transmissão
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/análise
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0909
[Cu] Atualização por classe:090824
[Lr] Data última revisão:
090824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090825
[St] Status:MEDLINE


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[PMID]:18180149
[Au] Autor:Brazier J; Chmelar D; Dubreuil L; Feierl G; Hedberg M; Kalenic S; Könönen E; Lundgren B; Malamou-Ladas H; Nagy E; Sullivan A; Nord CE; ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria
[Ad] Endereço:Division of Clinical Microbiology, F68, Karolinska University Hospital, Huddinge SE-141 86, Stockholm, Sweden. brazier@cardiff.ac.uk
[Ti] Título:European surveillance study on antimicrobial susceptibility of Gram-positive anaerobic cocci.
[So] Source:Int J Antimicrob Agents;31(4):316-20, 2008 Apr.
[Is] ISSN:0924-8579
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gram-positive anaerobic cocci (GPAC) are a heterogeneous group of microorganisms frequently isolated from local and systemic infections. In this study, the antimicrobial susceptibilities of clinical strains isolated in 10 European countries were investigated. After identification of 299 GPAC to species level, the minimum inhibitory concentrations of penicillin, imipenem, clindamycin, metronidazole, vancomycin and linezolid were determined by the agar dilution method according to the Clinical and Laboratory Standards Institute. The majority of isolates were identified as Finegoldia magna and Parvimonas micra (formerly Peptostreptococcus micros), isolated from skin and soft tissue infections. All isolates were susceptible to imipenem, metronidazole, vancomycin and linezolid. Twenty-one isolates (7%) were resistant to penicillin (n=13) and/or to clindamycin (n=12). Four isolates were resistant to both agents. The majority of resistant isolates were identified as F. magna and originated from blood, abscesses and soft tissue infections.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Cocos Anaeróbios Gram-Negativos/efeitos dos fármacos
Infecções por Bactérias Gram-Positivas/epidemiologia
Vigilância da População
[Mh] Termos MeSH secundário: Europa (Continente)/epidemiologia
Cocos Anaeróbios Gram-Negativos/enzimologia
Cocos Anaeróbios Gram-Negativos/genética
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Testes de Sensibilidade Microbiana
beta-Lactamases/biossíntese
beta-Lactamases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:0807
[Cu] Atualização por classe:080324
[Lr] Data última revisão:
080324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080109
[St] Status:MEDLINE
[do] DOI:10.1016/j.ijantimicag.2007.11.006


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Alves, Paulo Roberto Arruda
Texto completo SciELO Brasil
[PMID]:17334549
[Au] Autor:Zilberstein B; Quintanilha AG; Santos MA; Pajecki D; Moura EG; Alves PR; Maluf Filho F; de Souza JA; Gama-Rodrigues J
[Ad] Endereço:Digestive Surgery Division, Medical School, São Paulo University, São Paulo, SP, Brazil. brunozilb@uol.com.br
[Ti] Título:Digestive tract microbiota in healthy volunteers.
[So] Source:Clinics (Sao Paulo);62(1):47-54, 2007 Feb.
[Is] ISSN:1807-5932
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The aim of this study was to standardize the methods of sample collection of mucus from the digestive tract and to determine the microbiota in healthy volunteers from Brazil, collecting samples from the mouth, esophagus, stomach, duodenum, jejunum, ileum, colon, and rectum. METHODS: Microbiota of selected healthy volunteers from the oral cavity (n=10), the esophagus (n=10), the upper digestive tract (n=20), and the lower digestive tract (n=24) were evaluated through distinct collection methods. Collection methods took into account the different sites, using basic scraping and swabbing techniques, stimulated saliva from the oral cavity, irrigation-aspiration with sterile catheters especially designed for the esophagus, a probe especially designed for upper digestive tract, and a special catheter for the lower digestive tract. RESULTS: (i) Mixed microbiota were identified in the oral cavity, predominantly Gram-positive aerobic and anaerobic cocci; (ii) transitional flora mainly in the esophagus; (iii) Veillonella sp, Lactobacillus sp, and Clostridium sp in the stomach and duodenum; (iv) in the jejunum and upper ileum, we observed Bacteroides sp, Proteus sp, and Staphylococcus sp, in addition to Veillonella sp; (v) in the colon, the presence of "nonpathogenic" anaerobic bacteria Veillonella sp (average 10(5) UFC) indicates the existence of a low oxidation-reduction potential environment, which suggests the possibility of adoption of these bacteria as biological markers of total digestive tract health. CONCLUSIONS: The collection methods were efficient in obtaining adequate samples from each segment of the total digestive tract to reveal the normal microbiota. These procedures are safe and easily reproducible for microbiological studies.
[Mh] Termos MeSH primário: Bactérias Aeróbias/isolamento & purificação
Bactérias Anaeróbias/isolamento & purificação
Trato Gastrointestinal/microbiologia
Manejo de Espécimes/normas
[Mh] Termos MeSH secundário: Adulto
Distribuição de Qui-Quadrado
Colo/microbiologia
Contagem de Colônia Microbiana/métodos
Feminino
Cocos Anaeróbios Gram-Negativos/isolamento & purificação
Seres Humanos
Íleo/microbiologia
Masculino
Meia-Idade
Reto/microbiologia
Saliva/microbiologia
Manejo de Espécimes/métodos
Estatísticas não Paramétricas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:0708
[Cu] Atualização por classe:090520
[Lr] Data última revisão:
090520
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070306
[St] Status:MEDLINE


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[PMID]:17319435
[Au] Autor:Pavón Benito A; Asensio Martín MJ; de la Torre Campo A; Esparza Murillo I; Goldáraz Prados C; Salvador Bravo M
[Ad] Endereço:Servicio de Anestesiología y Reanimación, Hospital Virgen del Camino, Pamplona, Navarra. ruizpavon@yahoo.es
[Ti] Título:[Infectious spondylodiskitis and epidural abscess after spinal puncture for pilonidal sinus excision].
[Ti] Título:Espondilodiscitis infecciosa y absceso epidural después de una punción subaracnoidea para escisión de sinus pilonidal..
[So] Source:Rev Esp Anestesiol Reanim;54(1):49-53, 2007 Jan.
[Is] ISSN:0034-9356
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:Vertebral infections after spinal puncture are rare and often inadequately documented. Their incidence does not exceed that of spontaneous epidural abscesses and we should therefore be cautious about assuming a causal relation between puncture and an abscess. After analyzing 10 published cases we saw that only half of them reported on aseptic conditions and only 2 patients seem to have had a prior infection. In 3 cases, the abscesses appeared after technically simple punctures whereas half the reports did not even mention the type of puncture. This complication should be considered whenever a patient develops back pain and fever, even if there are no neurological deficits and even after a simple spinal puncture. Given that early diagnosis and treatment have proven effective in improving the survival rate and reducing the rate of neurological sequelae, magnetic resonance images should be ordered urgently so that early treatment can be established.
[Mh] Termos MeSH primário: Raquianestesia
Infecções por Bacteroides/etiologia
Discite/etiologia
Abscesso Epidural/etiologia
Infecções por Bactérias Gram-Negativas/etiologia
Vértebras Lombares
Seio Pilonidal/cirurgia
Punções/efeitos adversos
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/uso terapêutico
Bacteriemia/etiologia
Infecções por Bacteroides/diagnóstico
Infecções por Bacteroides/tratamento farmacológico
Infecções por Bacteroides/cirurgia
Terapia Combinada
Desbridamento
Discite/diagnóstico
Discite/tratamento farmacológico
Discite/cirurgia
Abscesso Epidural/diagnóstico
Abscesso Epidural/tratamento farmacológico
Abscesso Epidural/epidemiologia
Abscesso Epidural/cirurgia
Evolução Fatal
Febre/etiologia
Cocos Anaeróbios Gram-Negativos
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Infecções por Bactérias Gram-Negativas/cirurgia
Seres Humanos
Incidência
Dor Lombar/etiologia
Imagem por Ressonância Magnética
Masculino
Choque Séptico/etiologia
[Pt] Tipo de publicação:CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:0704
[Cu] Atualização por classe:091111
[Lr] Data última revisão:
091111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070227
[St] Status:MEDLINE


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[PMID]:12656175
[Au] Autor:Zoetendal EG; Plugge CM; Akkermans AD; de Vos WM
[Ad] Endereço:Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
[Ti] Título:Victivallis vadensis gen. nov., sp. nov., a sugar-fermenting anaerobe from human faeces.
[So] Source:Int J Syst Evol Microbiol;53(Pt 1):211-5, 2003 Jan.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel strictly anaerobic, cellobiose-degrading bacterium, strain CelloT, was isolated from a human faecal sample by combining enrichments in liquid and soft-agar basal media. A noteworthy characteristic was its inability to grow on normal agar plates and in roll tubes. The cells were coccus shaped and non-motile, with an extracellular slime layer. Growth of strain CelloT occurred between 20 and 40degrees C, with optimal growth at 37 degrees C. The pH range for growth was 5-7.5 with an optimum at 6.5. In pure culture, strain CelloT could only grow on a variety of sugars. Glucose was converted to acetate, ethanol and H2. The doubling time on glucose was 0.5 h. In a syntrophic co-culture with Methanospirillum hungatei strain JF-1T, strain CelloT converted glucose to acetate and H2. The G+C content was 59.2 mol%. 16S rDNA analysis revealed that the closest relatives of strain CelloT were two uncultured bacteria from anaerobic digesters, both with 94% 16S rDNA sequence similarity. The closest cultured representatives belong to genera of the bacterial division 'Verrucomicrobia'. The name Victivallis vadensis gen. nov., sp. nov. is proposed for strain CelloT (=DSM 14823T =ATCC BAA-548T).
[Mh] Termos MeSH primário: Cocos Anaeróbios Gram-Negativos/classificação
Cocos Anaeróbios Gram-Negativos/metabolismo
[Mh] Termos MeSH secundário: Composição de Bases
Metabolismo dos Carboidratos
Celobiose/metabolismo
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/genética
Fezes/microbiologia
Fermentação
Cocos Anaeróbios Gram-Negativos/genética
Cocos Anaeróbios Gram-Negativos/isolamento & purificação
Seres Humanos
Dados de Sequência Molecular
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 16462-44-5 (Cellobiose)
[Em] Mês de entrada:0306
[Cu] Atualização por classe:051117
[Lr] Data última revisão:
051117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030327
[St] Status:MEDLINE


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[PMID]:12916235
[Au] Autor:Kalachniuk LH; Vozna OIe; Kalachniuk HI; Lytsur IuM; Latsik NI; Savka OH; Marounek M; Kawai SH
[Ad] Endereço:Biotechnology Research Institute of Animal Production Gzhytskyi Lviv Academy of Veterinary Medicine, Ukraine.
[Ti] Título:[Metabolism of rumen microbial populations formed on biosubstrates with different assimilation under the effect of pentachlorophenol].
[Ti] Título:Metabolizm mikrobnykh populiatsii rubtsia, sformovanykh na biosubstratakh riznoï dostupnosti, za diï pentakhlorfenolu..
[So] Source:Ukr Biokhim Zh (1999);74(3):31-41, 2002 May-Jun.
[Cp] País de publicação:Ukraine
[La] Idioma:ukr
[Ab] Resumo:It has been established that metabolism of mixed microbial population formed on easy assimilated sources of energy and nitrogen (concentrate diet) progressed on higher level. There is increase of amilolytic activity, formation of lactate, ammonia, low molecular carbonic acids with predomination of propionate molar fraction. The increased resistance to effect of pentachlorophenol (PCP) is characteristic nature of the latter. The role of the most resistant synthrophic bacteria to PCP increases. The pure strains of Streptococcus bovis and Megasphaera elsdenii do not stop metabolism at 100 microM of PCP. Mixed population of microorganisms formed on hard accessible biosubstrates (cellulose) and Butyrivibrio fibrisolvens have the increased cellulosolytic activity and while the high sensibility even to low doses of PCP (10-40 microM) is observed. It has been supposed that mechanism of PCP effect is ambiguous for various species of microbial complex of rumen. It's effect strength on all main chains of metabolism (membrane transport, energetic exchange, protein biosynthesis, etc.) significantly depends on capacity of pool of metabolic intermediates formed as a result of definite program of biotechnology of nurture, but significantly decreases the harmful effect of biocides.
[Mh] Termos MeSH primário: Pentaclorofenol/farmacologia
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Celulose/metabolismo
Meios de Cultura
Relação Dose-Resposta a Droga
Cocos Anaeróbios Gram-Negativos/efeitos dos fármacos
Cocos Anaeróbios Gram-Negativos/metabolismo
Cocos Gram-Positivos/efeitos dos fármacos
Cocos Gram-Positivos/metabolismo
Lactatos/metabolismo
Rúmen/efeitos dos fármacos
Rúmen/metabolismo
Especificidade da Espécie
Streptococcus bovis/efeitos dos fármacos
Streptococcus bovis/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:COMPARATIVE STUDY; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Lactates); 9004-34-6 (Cellulose); D9BSU0SE4T (Pentachlorophenol)
[Em] Mês de entrada:0311
[Cu] Atualização por classe:150803
[Lr] Data última revisão:
150803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030815
[St] Status:MEDLINE


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[PMID]:10721476
[Au] Autor:Ziola B; Gee L; Berg NN; Lee SY
[Ad] Endereço:Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada. ziola@sask.usask.ca
[Ti] Título:Serogroups of the beer spoilage bacterium Megasphaera cerevisiae correlate with the molecular weight of the major EDTA-extractable surface protein.
[So] Source:Can J Microbiol;46(2):95-100, 2000 Feb.
[Is] ISSN:0008-4166
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Megasphaera cerevisiae is a Gram-negative obligate anaerobe that causes turbidity and off-flavour and aroma in beer. Seven isolates of M. cerevisiae were obtained worldwide, and their extractable surface antigens were focused upon to determine if there is more than one serogroup of this bacterium. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of ethylenediaminetetraacetic acid (EDTA) bacterial extracts revealed a predominant protein with apparent molecular weights of 46,000, 45,000, and 43,000 for three, two, and two isolates, respectively. When mouse anti-serum generated against any of the EDTA extracts was reacted with denatured bacterial proteins in immunoblots, all bacterial isolates exhibited extensive cross-reactivity involving three antigens, one being the major EDTA-extractable protein. In contrast, when the sera were tested for surface reactivity with intact bacteria, three cross-reactivity groups were observed, with the groups individually comprised of bacteria having the same size major EDTA-extractable surface protein. When BALB/c mice immunized with a bacterium from each of the three serogroups were used for monoclonal antibody (Mab) hybridoma production, bacterial surface-reactive Mabs were obtained whose reactivities parallel the three polyclonal antibody-defined serogroups. Through combining these surface-reactive Mabs, it will be possible to rapidly detect and identify beer contamination by M. cerevisiae belonging to any serogroup.
[Mh] Termos MeSH primário: Antígenos de Bactérias/isolamento & purificação
Cerveja/microbiologia
Cocos Anaeróbios Gram-Negativos/classificação
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/biossíntese
Anticorpos Monoclonais/biossíntese
Especificidade de Anticorpos
Antígenos de Bactérias/química
Antígenos de Bactérias/imunologia
Antígenos de Superfície
Ácido Edético
Eletroforese em Gel de Poliacrilamida
Fluorimunoensaio
Cocos Anaeróbios Gram-Negativos/imunologia
Hibridomas
Immunoblotting
Camundongos
Camundongos Endogâmicos BALB C
Peso Molecular
Sorotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antibodies, Monoclonal); 0 (Antigens, Bacterial); 0 (Antigens, Surface); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:0005
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000318
[St] Status:MEDLINE


  9 / 24 MEDLINE  
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[PMID]:10408096
[Au] Autor:Charest A; Bisaillon JG; Lépine F; Beaudet R
[Ad] Endereço:Centre de microbiologie et biotechnologie INRS-Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.
[Ti] Título:Removal of phenolic compounds from a petrochemical effluent with a methanogenic consortium.
[So] Source:Can J Microbiol;45(3):235-41, 1999 Mar.
[Is] ISSN:0008-4166
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:A methanogenic consortium was used to degrade phenol and ortho- (o-) cresol from a specific effluent of a petrochemical refinery. This effluent did not meet the local environmental regulations for phenolic compounds (178 mg/L), oils and greases (61 mg/L), ammoniacal nitrogen (75 mg/L) or sulfides (3.2 mg/L). The consortium, which degrades phenol via its carboxylation to benzoic acid, was progressively adapted to the effluent. Despite the very high effluent toxicity (EC50 of 2% with Microtox), the adapted consortium degraded 97% of 156 mg/L phenol in the supplemented effluent after 13 days in batch cultures (serum bottle). The addition of proteose peptone to the effluent is essential for phenol degradation. o-cresol was also transformed but not meta- or para-cresols. A continuous flow fixed-film anaerobic bioreactor was developed with the consortium. Treating the effluent with the bioreactor reduced phenol and phenolic compounds concentrations by 97 and 83%, respectively, for a hydraulic residence time of 6 h. This treatment also reduced by about half the effluent toxicity. Oils and greases and ammoniacal nitrogen were not affected. Similar microbiological forms were observed in serum bottles and in the bioreactors with or without the petrochemical effluent. These results indicate that this methanogenic consortium can treat efficiently the phenolic compounds in this specific petrochemical effluent.
[Mh] Termos MeSH primário: Bactérias Anaeróbias/metabolismo
Reatores Biológicos/microbiologia
Euryarchaeota/metabolismo
Fenóis/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cocos Anaeróbios Gram-Negativos/isolamento & purificação
Bacilos Gram-Positivos/isolamento & purificação
Peptonas
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Peptones); 0 (Phenols)
[Em] Mês de entrada:9908
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:990717
[St] Status:MEDLINE


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[PMID]:9620414
[Au] Autor:Rossmann SN; Wilson PH; Hicks J; Carter B; Cron SG; Simon C; Flaitz CM; Demmler GJ; Shearer WT; Kline MW
[Ad] Endereço:Baylor College of Medicine and Texas Children's Hospital, Houston 77030, USA. srossmann@msmail.path.tch.tmc.edu
[Ti] Título:Isolation of Lautropia mirabilis from oral cavities of human immunodeficiency virus-infected children.
[So] Source:J Clin Microbiol;36(6):1756-60, 1998 Jun.
[Is] ISSN:0095-1137
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lautropia mirabilis, a pleomorphic, motile, gram-negative coccus, has been isolated from the oral cavities of 32 of 60 (53.3%) children infected with human immunodeficiency virus (HIV) and 3 of 25 (12.0%) HIV-uninfected controls; the association of L. mirabilis isolation with HIV infection is significant (P < 0.001). All children in the study, both HIV-infected children and controls, were born to HIV-infected mothers. The presence of this bacterium was not associated with clinical disease in these children. The HIV-infected children with L. mirabilis did not differ from the HIV-infected children without L. mirabilis in immunological status, clinical status, or systemic medications. The role of HIV infection itself or concomitant factors in the establishment of L. mirabilis in the oral cavity remains to be elucidated.
[Mh] Termos MeSH primário: Gengiva/microbiologia
Cocos Anaeróbios Gram-Negativos/isolamento & purificação
Infecções por Bactérias Gram-Negativas/complicações
Infecções por HIV/complicações
Mucosa Bucal/microbiologia
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
Feminino
Cocos Anaeróbios Gram-Negativos/classificação
Cocos Anaeróbios Gram-Negativos/crescimento & desenvolvimento
Cocos Anaeróbios Gram-Negativos/ultraestrutura
Infecções por Bactérias Gram-Negativas/microbiologia
Seres Humanos
Lactente
Recém-Nascido
Masculino
Microscopia Eletrônica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Em] Mês de entrada:9807
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:980610
[St] Status:MEDLINE



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