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[PMID]:28007924
[Au] Autor:Balkus JE; Srinivasan S; Anzala O; Kimani J; Andac C; Schwebke J; Fredricks DN; McClelland RS
[Ad] Endereço:Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center , Seattle, Washington, USA.
[Ti] Título:Impact of Periodic Presumptive Treatment for Bacterial Vaginosis on the Vaginal Microbiome among Women Participating in the Preventing Vaginal Infections Trial.
[So] Source:J Infect Dis;215(5):723-731, 2017 03 01.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Evidence suggests that specific vaginal bacteria associated with bacterial vaginosis (BV) may increase the risk of adverse health outcomes in women. Among women participating in a randomized, double-blinded trial, we assessed the effect of periodic presumptive treatment (PPT) on detection of select vaginal bacteria. Methods: High-risk women from the United States and Kenya with a recent vaginal infection received intravaginal metronidazole 750 mg plus miconazole 200 mg or placebo for 5 consecutive nights each month for 12 months. Vaginal fluid specimens were collected via polyester/polyethylene terephthalate swabs every other month and tested for bacteria, using quantitative polymerase chain reaction (PCR) assays targeting the 16S ribosomal RNA gene. The effect of PPT on bacterium detection was assessed among all participants and stratified by country. Results: Of 234 women enrolled, 221 had specimens available for analysis. The proportion of follow-up visits with detectable quantities was lower in the PPT arm versus the placebo arm for the following bacteria: BVAB1, BVAB2, Atopobium vaginae, Leptotrichia/Sneathia, and Megasphaera. The magnitude of reductions was greater among Kenyan participants as compared to US participants. Conclusions: Use of monthly PPT for 1 year reduced colonization with several bacteria strongly associated with BV. The role of PPT to improve vaginal health should be considered, and efforts to improve the impact of PPT regimens are warranted.
[Mh] Termos MeSH primário: Metronidazol/administração & dosagem
Miconazol/administração & dosagem
Microbiota
Vagina/microbiologia
Vaginose Bacteriana/tratamento farmacológico
[Mh] Termos MeSH secundário: Actinobacteria/efeitos dos fármacos
Actinobacteria/isolamento & purificação
Administração Tópica
Adolescente
Adulto
Anti-Infecciosos/administração & dosagem
Anti-Infecciosos/uso terapêutico
Relação Dose-Resposta a Droga
Método Duplo-Cego
Feminino
Seguimentos
Seres Humanos
Quênia
Lactobacillus/efeitos dos fármacos
Lactobacillus/isolamento & purificação
Leptotrichia/efeitos dos fármacos
Leptotrichia/isolamento & purificação
Limite de Detecção
Modelos Lineares
Megasphaera/efeitos dos fármacos
Megasphaera/isolamento & purificação
Metronidazol/uso terapêutico
Miconazol/uso terapêutico
Meia-Idade
Manejo de Espécimes
Vaginose Bacteriana/prevenção & controle
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Infective Agents); 140QMO216E (Metronidazole); 7NNO0D7S5M (Miconazole)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170715
[Lr] Data última revisão:
170715
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jiw622


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[PMID]:27030383
[Au] Autor:Lim MY; Yoon HS; Rho M; Sung J; Song YM; Lee K; Ko G
[Ad] Endereço:Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea.
[Ti] Título:Analysis of the association between host genetics, smoking, and sputum microbiota in healthy humans.
[So] Source:Sci Rep;6:23745, 2016 Mar 31.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent studies showing clear differences in the airway microbiota between healthy and diseased individuals shed light on the importance of the airway microbiota in health. Here, we report the associations of host genetics and lifestyles such as smoking, alcohol consumption, and physical activity with the composition of the sputum microbiota using 16S rRNA gene sequence data generated from 257 sputum samples of Korean twin-family cohort. By estimating the heritability of each microbial taxon, we found that several taxa, including Providencia and Bacteroides, were significantly influenced by host genetic factors. Smoking had the strongest effect on the overall microbial community structure among the tested lifestyle factors. The abundances of Veillonella and Megasphaera were higher in current-smokers, and increased with the pack-year value and the Fagerstrom Test of Nicotine Dependence (FTND) score. In contrast, Haemophilus decreased with the pack-year of smoking and the FTND score. Co-occurrence network analysis showed that the taxa were clustered according to the direction of associations with smoking, and that the taxa influenced by host genetics were found together. These results demonstrate that the relationships among sputum microbial taxa are closely associated with not only smoking but also host genetics.
[Mh] Termos MeSH primário: Consumo de Bebidas Alcoólicas/genética
Microbiota/genética
Fumar/genética
Escarro/microbiologia
Tabagismo/genética
[Mh] Termos MeSH secundário: Adulto
Bacteroides/classificação
Bacteroides/genética
Exercício/fisiologia
Feminino
Interação Gene-Ambiente
Haemophilus/classificação
Haemophilus/genética
Seres Humanos
Masculino
Megasphaera/classificação
Megasphaera/genética
Meia-Idade
RNA Ribossômico 16S/genética
Tabagismo/microbiologia
Veillonella/classificação
Veillonella/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160401
[St] Status:MEDLINE
[do] DOI:10.1038/srep23745


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[PMID]:26409956
[Au] Autor:Minuti A; Palladino A; Khan MJ; Alqarni S; Agrawal A; Piccioli-Capelli F; Hidalgo F; Cardoso FC; Trevisi E; Loor JJ
[Ad] Endereço:Istituto di Zootecnica, Facoltà di Scienze Agrarie, Alimentari e Ambientali, Università Cattolica del Sacro Cuore, Piacenza, 29122, Italy.
[Ti] Título:Abundance of ruminal bacteria, epithelial gene expression, and systemic biomarkers of metabolism and inflammation are altered during the peripartal period in dairy cows.
[So] Source:J Dairy Sci;98(12):8940-51, 2015 Dec.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Seven multiparous Holstein cows with a ruminal fistula were used to investigate the changes in rumen microbiota, gene expression of the ruminal epithelium, and blood biomarkers of metabolism and inflammation during the transition period. Samples of ruminal digesta, biopsies of ruminal epithelium, and blood were obtained during -14 through 28d in milk (DIM). A total of 35 genes associated with metabolism, transport, inflammation, and signaling were evaluated by quantitative reverse transcription-PCR. Among metabolic-related genes, expression of HMGCS2 increased gradually from -14 to a peak at 28 DIM, underscoring its central role in epithelial ketogenesis. The decrease of glucose and the increase of nonesterified fatty acids and ß-hydroxybutyrate in the blood after calving confirmed the state of negative energy balance. Similarly, increases in bilirubin and decreases in albumin concentrations after calving were indicative of alterations in liver function and inflammation. Despite those systemic signs, lower postpartal expression of TLR2, TLR4, CD45, and NFKB1 indicated the absence of inflammation within the epithelium. Alternatively, these could reflect an adaptation to react against inducers of the immune system arising in the rumen (e.g., bacterial endotoxins). The downregulation of RXRA, INSR, and RPS6KB1 between -14 and 10 DIM indicated a possible increase in insulin resistance. However, the upregulation of IRS1 during the same time frame could serve to restore sensitivity to insulin of the epithelium as a way to preserve its proliferative capacity. The upregulation of TGFB1 from -14 and 10 DIM coupled with upregulation of both EGFR and EREG from 10 to 28 DIM indicated the existence of 2 waves of epithelial proliferation. However, the downregulation of TGFBR1 from -14 through 28 DIM indicated some degree of cell proliferation arrest. The downregulation of OCLN and TJP1 from -14 to 10 DIM indicated a loss of tight-junction integrity. The gradual upregulation of membrane transporters MCT1 and UTB to peak levels at 28 DIM reflected the higher intake and fermentability of the lactation diet. In addition, those changes in the diet after calving resulted in an increase of butyrate and a decrease of ruminal pH and acetate, which partly explain the increase of Anaerovibrio lipolytica, Prevotella bryantii, and Megasphaera elsdenii and the decrease of fibrolytic bacteria (Fibrobacter succinogenes, Butyrivibrio proteoclasticus). Overall, these multitier changes revealed important features associated with the transition into lactation. Alterations in ruminal epithelium gene expression could be driven by nutrient intake-induced changes in microbes; microbial metabolism; and the systemic metabolic, hormonal, and immune changes. Understanding causes and mechanisms driving the interaction among ruminal bacteria and host immunometabolic responses merits further study.
[Mh] Termos MeSH primário: Epitélio/metabolismo
Microbioma Gastrointestinal
Expressão Gênica
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/sangue
Animais
Biomarcadores/sangue
Glicemia/metabolismo
Butyrivibrio/isolamento & purificação
Bovinos
Proliferação Celular
Regulação para Baixo
Ingestão de Energia
Metabolismo Energético
Ácidos Graxos não Esterificados/sangue
Feminino
Fermentação
Fibrobacter/isolamento & purificação
Concentração de Íons de Hidrogênio
Hidroximetilglutaril-CoA Sintase/genética
Hidroximetilglutaril-CoA Sintase/metabolismo
Inflamação/veterinária
Insulina/metabolismo
Proteínas Substratos do Receptor de Insulina/genética
Proteínas Substratos do Receptor de Insulina/metabolismo
Resistência à Insulina
Lactação
Antígenos Comuns de Leucócito/genética
Antígenos Comuns de Leucócito/metabolismo
Megasphaera/isolamento & purificação
Leite/química
Leite/secreção
Subunidade p50 de NF-kappa B/genética
Subunidade p50 de NF-kappa B/metabolismo
Prevotella/isolamento & purificação
Receptor do Fator de Crescimento Epidérmico/genética
Receptor do Fator de Crescimento Epidérmico/metabolismo
Receptor de Insulina/genética
Receptor de Insulina/metabolismo
Proteínas Quinases S6 Ribossômicas 70-kDa/genética
Proteínas Quinases S6 Ribossômicas 70-kDa/metabolismo
Receptor 2 Toll-Like/genética
Receptor 2 Toll-Like/metabolismo
Receptor 4 Toll-Like/genética
Receptor 4 Toll-Like/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Biomarkers); 0 (Blood Glucose); 0 (Fatty Acids, Nonesterified); 0 (Insulin); 0 (Insulin Receptor Substrate Proteins); 0 (NF-kappa B p50 Subunit); 0 (Toll-Like Receptor 2); 0 (Toll-Like Receptor 4); EC 2.3.3.10 (Hydroxymethylglutaryl-CoA Synthase); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.10.1 (Receptor, Insulin); EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa); EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 1); EC 3.1.3.48 (Leukocyte Common Antigens); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150928
[St] Status:MEDLINE


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[PMID]:26299164
[Au] Autor:Harder H; Khol-Parisini A; Metzler-Zebeli BU; Klevenhusen F; Zebeli Q
[Ad] Endereço:Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine Vienna, 1210 Vienna, Austria; Research Cluster Animal Gut Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
[Ti] Título:Treatment of grain with organic acids at 2 different dietary phosphorus levels modulates ruminal microbial community structure and fermentation patterns in vitro.
[So] Source:J Dairy Sci;98(11):8107-20, 2015 Nov.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent data indicate positive effects of treating grain with citric (CAc) or lactic acid (LAc) on the hydrolysis of phytate phosphorus (P) and fermentation products of the grain. This study used a semicontinuous rumen simulation technique to evaluate the effects of processing of barley with 50.25 g/L (wt/vol) CAc or 76.25 g/L LAc on microbial composition, metabolic fermentation profile, and nutrient degradation at low or high dietary P supply. The low P diet [3.1g of P per kg of dry matter (DM) of dietary P sources only] was not supplemented with inorganic P, whereas the high P diet was supplemented with 0.5 g of inorganic P per kg of DM through mineral premix and 870 mg of inorganic P/d per incubation fermenter via artificial saliva. Target microbes were determined using quantitative PCR. Data showed depression of total bacteria but not of total protozoa or short-chain fatty acid (SCFA) concentration with the low P diet. In addition, the low P diet lowered the relative abundance of Ruminococcus albus and decreased neutral detergent fiber (NDF) degradation and acetate proportion, but increased the abundance of several predominantly noncellulolytic bacterial species and anaerobic fungi. Treatment of grain with LAc increased the abundance of total bacteria in the low P diet only, and this effect was associated with a greater concentration of SCFA in the ruminal fluid. Interestingly, in the low P diet, CAc treatment of barley increased the most prevalent bacterial group, the genus Prevotella, in ruminal fluid and increased NDF degradation to the same extent as did inorganic P supplementation in the high P diet. Treatment with either CAc or LAc lowered the abundance of Megasphaera elsdenii but only in the low P diet. On the other hand, CAc treatment increased the proportion of acetate in the low P diet, whereas LAc treatment decreased this variable at both dietary P levels. The propionate proportion was significantly increased by LAc at both P levels, whereas butyrate increased only with the low P diet. Treatments with CAc or LAc reduced the degradation of CP and ammonia concentration compared with the control diet at both P levels. In conclusion, the beneficial effects of CAc and LAc treatment on specific ruminal microbes, fermentation profile, and fiber degradation in the low P diet suggest the potential for the treatment to compensate for the lack of inorganic P supplementation in vitro. Further research is warranted to determine the extent to which the treatment can alleviate the shortage of inorganic P supplementation under in vivo conditions.
[Mh] Termos MeSH primário: Ácido Cítrico/análise
Grãos Comestíveis
Microbioma Gastrointestinal/efeitos dos fármacos
Ácido Láctico/análise
Fósforo na Dieta/análise
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Acetatos/análise
Ração Animal/análise
Animais
Dieta/veterinária
Fibras na Dieta/análise
Ácidos Graxos Voláteis/análise
Fermentação
Hordeum
Concentração de Íons de Hidrogênio
Megasphaera/isolamento & purificação
Prevotella/isolamento & purificação
Rúmen/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (Dietary Fiber); 0 (Fatty Acids, Volatile); 0 (Phosphorus, Dietary); 2968PHW8QP (Citric Acid); 33X04XA5AT (Lactic Acid)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150825
[St] Status:MEDLINE


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[PMID]:26298766
[Au] Autor:Weimer PJ; Da Silva Cabral L; Cacite F
[Ad] Endereço:USDA-Agricultural Research Service, US Dairy Forage Research Center, Madison, WI 53706; Department of Bacteriology, University of Wisconsin, Madison 53706. Electronic address: Paul.Weimer@ars.usda.gov.
[Ti] Título:Effects of ruminal dosing of Holstein cows with Megasphaera elsdenii on milk fat production, ruminal chemistry, and bacterial strain persistence.
[So] Source:J Dairy Sci;98(11):8078-92, 2015 Nov.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Megasphaera elsdenii is a lactate-utilizing bacterium whose ruminal abundance has been shown to be greatly elevated during milk fat depression (MFD). To further examine this association, a total of 23 cannulated multiparous Holstein cows were examined in 3 experiments in which strains of M. elsdenii were directly dosed into the rumen (~2 × 10(12) cells/dose); control cows were dosed with sterile lactate-free culture medium. Cows were fed a total mixed ration (292 g of starch/kg of dry matter) that contained primarily corn silage, alfalfa silage, finely ground high-moisture corn, supplemental protein, and corn oil (3 g/kg of dry matter). Experiments differed in stage of lactation of the cows (early or late), dosing events (single dose, or 4 doses over a 5-d period), timing of dose (prefeed or 4 h postfeed), and M. elsdenii strain (laboratory strain YI9 or 3 strains isolated from cows in the same herd). Dry matter intake and milk yield and composition were measured from 5 to 0 d before dosing and 1 to 7d after first dosing, plus later time points that varied by experiment. Milk yield and composition were not affected by dosing. Megasphaera elsdenii was quantified in the liquid phase of ruminal contents by automated ribosomal intergenic spacer analysis, or by PCR with relative quantification (M. elsdenii 16S rRNA gene copy number as a percentage of total bacterial 16S rRNA gene copies). Neither the M. elsdenii-dosed or control cows displayed MFD after dosing, and in almost all cases M. elsdenii populations returned to low baseline levels (<0.02% of 16S rRNA gene copy number) within 24 h of dosing. This rapid decline in M. elsdenii also occurred in several cows that were dosed with a strain of M. elsdenii that had been isolated from that particular cow during a previous bout of MFD. Ruminal pH and total millimolar volatile fatty acids and lactate did not differ between dosed and control cows, although acetate-to-propionate ratio declined in both groups and butyrate increased after dosing with M. elsdenii. The results confirm that establishing exogenously added bacterial strains in the rumen is difficult, even for strains previously isolated from the recipient cow. The potential role of M. elsdenii as an agent of MFD remains unclear in the absence of successful establishment of the dosed strains.
[Mh] Termos MeSH primário: Bovinos/microbiologia
Megasphaera/fisiologia
Leite/química
Rúmen/química
[Mh] Termos MeSH secundário: Acetatos/análise
Animais
Bovinos/fisiologia
DNA Bacteriano/genética
Indústria de Laticínios
Dieta/veterinária
Ácidos Graxos Voláteis/análise
Feminino
Lactação
Medicago sativa
Megasphaera/genética
Propionatos/análise
RNA Ribossômico/genética
RNA Ribossômico 16S/genética
Rúmen/microbiologia
Silagem/análise
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Acetates); 0 (DNA, Bacterial); 0 (Fatty Acids, Volatile); 0 (Propionates); 0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151018
[Lr] Data última revisão:
151018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150824
[St] Status:MEDLINE


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[PMID]:26168961
[Au] Autor:Neubauer S; Chu DB; Marx H; Sauer M; Hann S; Koellensperger G
[Ad] Endereço:Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Life Sciences-BOKU, 1190, Vienna, Austria.
[Ti] Título:LC-MS/MS-based analysis of coenzyme A and short-chain acyl-coenzyme A thioesters.
[So] Source:Anal Bioanal Chem;407(22):6681-8, 2015 Sep.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Absolute quantification of intracellular coenzyme A (CoA), coenzyme A disulfide, and short-chain acyl-coenzyme A thioesters was addressed by developing a tailored metabolite profiling method based on liquid chromatography in combination with tandem mass spectrometric detection (LC-MS/MS). A reversed phase chromatographic separation was established which is capable of separating a broad spectrum of CoA, its corresponding derivatives, and their isomers despite the fact that no ion-pairing reagent was used (which was considered as a key advantage of the method). Excellent analytical figures of merit such as high sensitivity (LODs in the nM to sub-nM range) and high repeatability (routinely 4 %; N = 15) were obtained. Method validation comprised a study on standard purity, stability, and recoveries during sample preparation. Uniformly labeled U(13)C yeast cell extracts offered ideal internal standards for validation purposes and for a quantification exercise in the rumen bacterium Megasphaera elsdenii.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Coenzima A/metabolismo
Megasphaera/metabolismo
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Coenzima A/análise
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
31664-36-5 (coenzyme A disulfide); SAA04E81UX (Coenzyme A)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150822
[Lr] Data última revisão:
150822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150715
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-8825-9


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[PMID]:26156825
[Au] Autor:Nelson DB; Hanlon AL; Wu G; Liu C; Fredricks DN
[Ad] Endereço:Department of Obstetrics and Gynecology, Temple University, Philadelphia, PA, USA. dnelson@temple.edu.
[Ti] Título:First Trimester Levels of BV-Associated Bacteria and Risk of Miscarriage Among Women Early in Pregnancy.
[So] Source:Matern Child Health J;19(12):2682-7, 2015 Dec.
[Is] ISSN:1573-6628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Prior studies have examined the role of bacterial vaginosis (BV) and increased risk of miscarriage; however the risk has been modest and many BV positive pregnant women deliver at term. BV is microbiologically heterogeneous, and thus the identification of specific BV-associated bacteria associated with miscarriage is warranted. METHODS: We measured the presence and level of seven BV-associated bacteria prior to 14 weeks gestation among urban pregnant women seeking routine prenatal care at five urban obstetric practices at Temple University Hospital in Philadelphia PA from July 2008 through September 2011. 418 Pregnant women were included in this assessment and 74 experienced a miscarriage. RESULTS: Mean log concentration of BVAB3 was significantly higher among women experiencing a miscarriage (4.27 vs. 3.71, p value = 0.012). Younger women with high levels of BVAB3 had the greatest risk of miscarriage. In addition, we found a significant decreased risk of miscarriage among women with higher log concentrations of Leptotrichia/Sneathia species or Megasphaera phylotype 1-like species early in pregnancy. CONCLUSIONS FOR PRACTICE: The identification of selected vaginal bacteria associated with an increased risk of miscarriage could support screening programs early in pregnancy and promote early therapies to reduce early pregnancy loss.
[Mh] Termos MeSH primário: Aborto Espontâneo/epidemiologia
Primeiro Trimestre da Gravidez/fisiologia
Vaginose Bacteriana/epidemiologia
[Mh] Termos MeSH secundário: Aborto Espontâneo/microbiologia
Feminino
Avaliação do Impacto na Saúde/utilização
Seres Humanos
Leptotrichia/patogenicidade
Megasphaera/patogenicidade
Gravidez
Vaginose Bacteriana/complicações
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150710
[St] Status:MEDLINE
[do] DOI:10.1007/s10995-015-1790-2


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[PMID]:26123320
[Au] Autor:Rico DE; Preston SH; Risser JM; Harvatine KJ
[Ad] Endereço:Department of Animal Science,Penn State University, University Park,301 Henning Building,PA16802,USA.
[Ti] Título:Rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows.
[So] Source:Br J Nutr;114(3):358-67, 2015 Aug 14.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ruminant provides a powerful model for understanding the temporal dynamics of gastrointestinal microbial communities. Diet-induced milk fat depression (MFD) in the dairy cow is caused by rumen-derived bioactive fatty acids, and is commonly attributed to the changes in the microbial population. The aim of the present study was to determine the changes occurring in nine ruminal bacterial taxa with well-characterised functions, and abundance of total fungi, ciliate protozoa and bacteria during the induction of and recovery from MFD. Interactions between treatment and time were observed for ten of the twelve populations. The total number of both fungi and ciliate protozoa decreased rapidly (days 4 and 8, respectively) by more than 90% during the induction period and increased during the recovery period. The abundance of Streptococcus bovis (amylolytic) peaked at 350% of control levels on day 4 of induction and rapidly decreased during the recovery period. The abundance of Prevotella bryantii (amylolytic) decreased by 66% from day 8 to 20 of the induction period and increased to the control levels on day 12 of the recovery period. The abundance of Megasphaera elsdenii and Selenomonas ruminantium (lactate-utilising bacteria) increased progressively until day 12 of induction (>170%) and decreased during the recovery period. The abundance of Fibrobacter succinogenes (fibrolytic) decreased by 97% on day 4 of induction and increased progressively to an equal extent during the recovery period, although smaller changes were observed for other fibrolytic bacteria. The abundance of the Butyrivibrio fibrisolvens/Pseudobutyrivibrio group decreased progressively during the induction period and increased during the recovery period, whereas the abundance of Butyrivibrio hungatei was not affected by treatment. Responsive taxa were modified rapidly, with the majority of changes occurring within 8 d and their time course was similar to the time course of the induction of MFD, demonstrating a strong correlation between changes in ruminal microbial populations and MFD.
[Mh] Termos MeSH primário: Dieta/veterinária
Gorduras/análise
Leite/química
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Animais
Bactérias/classificação
Bactérias/isolamento & purificação
Bactérias/metabolismo
Carga Bacteriana
Butyrivibrio/isolamento & purificação
Butyrivibrio/metabolismo
Bovinos
Dieta/efeitos adversos
Ácidos Graxos/biossíntese
Ácidos Graxos/farmacologia
Feminino
Fibrobacter/isolamento & purificação
Fibrobacter/metabolismo
Lactação
Lipídeos
Megasphaera/isolamento & purificação
Megasphaera/metabolismo
Microbiota/fisiologia
Prevotella/isolamento & purificação
Prevotella/metabolismo
Selenomonas/isolamento & purificação
Selenomonas/metabolismo
Streptococcus bovis/isolamento & purificação
Streptococcus bovis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Fats); 0 (Fatty Acids); 0 (Lipids)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151022
[Lr] Data última revisão:
151022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150701
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114515001865


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[PMID]:26081163
[Au] Autor:Zhou A; Liu W; Varrone C; Wang Y; Wang A; Yue X
[Ad] Endereço:College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, China.
[Ti] Título:Evaluation of surfactants on waste activated sludge fermentation by pyrosequencing analysis.
[So] Source:Bioresour Technol;192:835-40, 2015 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of three widely-used surfactants on waste activated sludge (WAS) fermentation and microbial community structures were investigated. Rhamnolipid bio-surfactants (RL) showed more positive effects on WAS hydrolysis and acidification compared to chemosynthetic surfactants, such as sodium dodecylsulphate (SDS) and sodium dodecyl benzene sulfonate (SDBS). The highest SCOD and VFAs concentrations obtained with RL were 1.15-fold and 1.16-fold that of SDS, and up to 1.73 and 3.63 times higher than those obtained with SDBS. Pyrosequencing analysis showed that an evident reduction in bacterial diversity in surfactant-treated WAS. Moreover, acid-producing bacteria (such as Megasphaera and Oscillibacter), detected with RL, were (6.8% and 6.4% in proportion) more abundant than with SDS, and were rarely found in SDBS and the control. The results also revealed that RL allowed efficient hydrolysis enhancement and was favorable to functional microorganisms for further acidification during WAS fermentation.
[Mh] Termos MeSH primário: Firmicutes/fisiologia
Megasphaera/fisiologia
Eliminação de Resíduos/métodos
Análise de Sequência de DNA/métodos
Esgotos/química
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Sequência de Bases
Fermentação
Firmicutes/isolamento & purificação
Genoma Bacteriano/genética
Megasphaera/isolamento & purificação
Dados de Sequência Molecular
Tensoativos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sewage); 0 (Surface-Active Agents)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150710
[Lr] Data última revisão:
150710
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150618
[St] Status:MEDLINE


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[PMID]:25903584
[Au] Autor:Chowdhury NP; Kahnt J; Buckel W
[Ad] Endereço:Laboratorium für Mikrobiologie, Fachbereich Biologie and Synmikro, Philipps-Universität, Marburg, Germany.
[Ti] Título:Reduction of ferredoxin or oxygen by flavin-based electron bifurcation in Megasphaera elsdenii.
[So] Source:FEBS J;282(16):3149-60, 2015 Aug.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Over 50 years ago, it was reported that, in the anaerobic rumen bacterium Megasphaera elsdenii, the reduction of crotonyl-CoA to butyryl-CoA by NADH involved an electron transferring flavoprotein (Etf) as mediator [Baldwin RL, Milligan LP (1964) Biochim Biophys Acta 92, 421-432]. Purification and spectroscopic characterization revealed that this Etf contained 2 FAD, whereas, in the Etfs from aerobic and facultative bacteria, one FAD is replaced by AMP. Recently we detected a similar system in the related anaerobe Acidaminococcus fermentans that differed in the requirement of additional ferredoxin as electron acceptor. The whole process was established as flavin-based electron bifurcation in which the exergonic reduction of crotonyl-CoA by NADH mediated by Etf + butyryl-CoA dehydrogenase (Bcd) was coupled to the endergonic reduction of ferredoxin also by NADH. In the present study, we demonstrate that, under anaerobic conditions, Etf + Bcd from M. elsdenii bifurcate as efficiently as Etf + Bcd from A. fermentans. Under the aerobic conditions used in the study by Baldwin and Milligan and in the presence of catalytic amounts of crotonyl-CoA or butyryl-CoA, however, Etf + Bcd act as NADH oxidase producing superoxide and H2 O2 , whereas ferredoxin is not required. We hypothesize that, during bifurcation, oxygen replaces ferredoxin to yield superoxide. In addition, the formed butyryl-CoA is re-oxidized by a second oxygen molecule to crotonyl-CoA, resulting in a stoichiometry of 2 NADH consumed and 2 H2 O2 formed. As a result of the production of reactive oxygen species, electron bifurcation can be regarded as an Achilles' heel of anaerobes when exposed to air.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Flavoproteínas Transferidoras de Elétrons/metabolismo
Ferredoxinas/metabolismo
Megasphaera/metabolismo
[Mh] Termos MeSH secundário: Acidaminococcus/genética
Acidaminococcus/metabolismo
Anaerobiose
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Butiril-CoA Desidrogenase/química
Butiril-CoA Desidrogenase/genética
Butiril-CoA Desidrogenase/metabolismo
Transporte de Elétrons
Flavoproteínas Transferidoras de Elétrons/química
Flavoproteínas Transferidoras de Elétrons/genética
Megasphaera/genética
Complexos Multienzimáticos/química
Complexos Multienzimáticos/genética
Complexos Multienzimáticos/metabolismo
NAD/metabolismo
NADH NADPH Oxirredutases/química
NADH NADPH Oxirredutases/genética
NADH NADPH Oxirredutases/metabolismo
Oxirredução
Oxigênio/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Espectrofotometria
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Electron-Transferring Flavoproteins); 0 (Ferredoxins); 0 (Multienzyme Complexes); 0 (Recombinant Proteins); 0U46U6E8UK (NAD); EC 1.3.8.1 (Butyryl-CoA Dehydrogenase); EC 1.6.- (NADH oxidase); EC 1.6.- (NADH, NADPH Oxidoreductases); S88TT14065 (Oxygen)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150818
[Lr] Data última revisão:
150818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150424
[St] Status:MEDLINE
[do] DOI:10.1111/febs.13308



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