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[PMID]:28727856
[Au] Autor:da Silva VM; Sato JAP; Araujo JN; Squina FM; Muniz JRC; Riske KA; Garcia W
[Ad] Endereço:Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, São Paulo, Brazil.
[Ti] Título:Systematic studies of the interactions between a model polyphenol compound and microbial ß-glucosidases.
[So] Source:PLoS One;12(7):e0181629, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lignin is a major obstacle for cost-effective conversion of cellulose into fermentable sugars. Non-productive adsorption onto insoluble lignin fragments and interactions with soluble phenols are important inhibition mechanisms of cellulases, including ß-glucosidases. Here, we examined the inhibitory effect of tannic acid (TAN), a model polyphenolic compound, on ß-glucosidases from the bacterium Thermotoga petrophila (TpBGL1 and TpBGL3) and archaeon Pyrococcus furiosus (PfBGL1). The results revealed that the inhibition effects on ß-glucosidases were TAN concentration-dependent. TpBGL1 and TpBGL3 were more tolerant to the presence of TAN when compared with PfBGL1, while TpBGL1 was less inhibited when compared with TpBGL3. In an attempt to better understand the inhibitory effect, the interaction between TAN and ß-glucosidases were analyzed by isothermal titration calorimetry (ITC). Furthermore, the exposed hydrophobic surface areas in ß-glucosidases were analyzed using a fluorescent probe and compared with the results of inhibition and ITC. The binding constants determined by ITC for the interactions between TAN and ß-glucosidases presented the same order of magnitude. However, the number of binding sites and exposed hydrophobic surface areas varied for the ß-glucosidases studied. The binding between TAN and ß-glucosidases were driven by enthalpic effects and with an unfavorable negative change in entropy upon binding. Furthermore, the data suggest that there is a high correlation between exposed hydrophobic surface areas and the number of binding sites on the inhibition of microbial ß-glucosidases by TAN. These studies can be useful for biotechnological applications.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/enzimologia
Pyrococcus furiosus/enzimologia
Taninos/farmacologia
beta-Glucosidase/metabolismo
[Mh] Termos MeSH secundário: Proteínas Arqueais/antagonistas & inibidores
Proteínas Arqueais/química
Proteínas Arqueais/genética
Proteínas Arqueais/metabolismo
Proteínas de Bactérias/antagonistas & inibidores
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Calorimetria
Relação Dose-Resposta a Droga
Escherichia coli
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/química
Interações Hidrofóbicas e Hidrofílicas
Modelos Moleculares
Ligação Proteica
Pyrococcus furiosus/química
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Tensoativos/farmacologia
beta-Glucosidase/antagonistas & inibidores
beta-Glucosidase/química
beta-Glucosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Bacterial Proteins); 0 (Enzyme Inhibitors); 0 (Recombinant Proteins); 0 (Surface-Active Agents); 0 (Tannins); EC 3.2.1.21 (beta-Glucosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181629


  2 / 200 MEDLINE  
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[PMID]:27928679
[Au] Autor:Han D; Xu Z
[Ad] Endereço:Department of Biological Sciences, Bowling Green State University, Bowling Green, OH, 43403, USA.
[Ti] Título:Development of a pyrE-based selective system for Thermotoga sp. strain RQ7.
[So] Source:Extremophiles;21(2):297-306, 2017 Mar.
[Is] ISSN:1433-4909
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:To fully unlock the biotechnological potentials of Thermotoga species, this study aimed to expand the genetic toolbox of Thermotoga by developing a new selective system. The developed system was composed of two components: a recipient strain bearing a deletion in its orotate phosphoribosyltransferase gene pyrE and a shuttle vector expressing a heterologous pyrE as the selectable marker. A spontaneous uracil auxotroph, T. sp. strain RQ7-15, was isolated at 70 °C with 2 mg/ml 5-fluoroorotic acid. The mutant carried a 112 bp deletion in pyrE and was a suitable recipient strain. To avoid homologous recombination, the pyrE gene from another thermophilic bacterium Caldicellulosiruptor saccharolyticus was used as the selectable marker. The gene was cloned into two Thermotoga-E. coli shuttle vectors, controlled by different promoters: the promoter of Thermus S-layer protein (P ) in pDH25 and the promoter of the pyrimidine synthesis operon of T. sp. strain RQ7 (P ) in pDH28. After being introduced into the mutant strain RQ7-15 through natural transformation, both vectors allowed the host to thrive in a minimal medium. Single colonies of transformants were isolated and confirmed by polymerase chain reactions and restriction digestions. In summary, a pyrE-based selective system has been established in T. sp. strain RQ7.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Óperon
Orotato Fosforribosiltransferase/genética
Regiões Promotoras Genéticas/genética
[Mh] Termos MeSH secundário: Marcadores Genéticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Genetic Markers); EC 2.4.2.10 (Orotate Phosphoribosyltransferase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE
[do] DOI:10.1007/s00792-016-0902-2


  3 / 200 MEDLINE  
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[PMID]:27871370
[Au] Autor:Okano H; Katano Y; Baba M; Fujiwara A; Hidese R; Fujiwara S; Yanagihara I; Hayashi T; Kojima K; Takita T; Yasukawa K
[Ad] Endereço:Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
[Ti] Título:Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase.
[So] Source:Enzyme Microb Technol;96:111-120, 2017 Jan.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Detection of mRNA is a valuable method for monitoring the specific gene expression. In this study, we devised a novel cDNA synthesis method using three enzymes, the genetically engineered thermostable variant of reverse transcriptase (RT), MM4 (E286R/E302K/L435R/D524A) from Moloney murine leukemia virus (MMLV), the genetically engineered variant of family A DNA polymerase with RT activity, K4pol from thermophilic Thermotoga petrophila K4, and the DNA/RNA helicase Tk-EshA from a hyperthermophilic archaeon Thermococcus kodakarensis. By optimizing assay conditions for three enzymes using Taguchi's method, 100 to 1000-fold higher sensitivity was achieved for cDNA synthesis than conventional assay condition using only RT. Our results suggest that DNA polymerase with RT activity and DNA/RNA helicase are useful to increase the sensitivity of cDNA synthesis.
[Mh] Termos MeSH primário: DNA Complementar/biossíntese
DNA Complementar/genética
RNA/análise
RNA/genética
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Helicases/genética
DNA Polimerase Dirigida por DNA/genética
DNA Polimerase Dirigida por DNA/metabolismo
Estabilidade Enzimática
Expressão Gênica
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/enzimologia
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Vírus da Leucemia Murina de Moloney/enzimologia
Vírus da Leucemia Murina de Moloney/genética
Análise de Sequência com Séries de Oligonucleotídeos
Engenharia de Proteínas
RNA Helicases/genética
DNA Polimerase Dirigida por RNA/genética
DNA Polimerase Dirigida por RNA/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Temperatura Ambiente
Thermococcus/enzimologia
Thermococcus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Recombinant Proteins); 63231-63-0 (RNA); EC 2.7.7.49 (RNA-Directed DNA Polymerase); EC 2.7.7.7 (DNA-Directed DNA Polymerase); EC 3.6.4.- (DNA Helicases); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE


  4 / 200 MEDLINE  
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[PMID]:27209035
[Au] Autor:Wagschal K; Rose Stoller J; Chan VJ; Lee CC; Grigorescu AA; Jordan DB
[Ad] Endereço:USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA, 94710, USA. kurt.wagschal@ars.usda.gov.
[Ti] Título:Expression and Characterization of Hyperthermostable Exo-polygalacturonase TtGH28 from Thermotoga thermophilus.
[So] Source:Mol Biotechnol;58(7):509-19, 2016 Jul.
[Is] ISSN:1559-0305
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:D-galacturonic acid is a potential platform chemical comprising the principal component of pectin in the citrus processing waste stream. Several enzyme activities are required for the enzymatic production of galacturonic acid from pectin, including exo- and endo-polygalacturonases. The gene TtGH28 encoding a putative GH28 polygalacturonase from Pseudothermotoga thermarum DSM 5069 (Theth_0397, NCBI# AEH50492.1) was synthesized, expressed in Escherichia coli, and characterized. Alignment of the amino acid sequence of gene product TtGH28 with other GH28 proteins whose structures and details of their catalytic mechanism have been elucidated shows that three catalytic Asp residues and several other key active site residues are strictly conserved. Purified TtGH28 was dimeric and hyperthermostable, with K t (0.5)  = 86.3 °C. Kinetic parameters for activity on digalacturonic acid, trigalacturonic acid, and polygalacturonic acid were obtained. No substrate inhibition was observed for polygalacturonate, while the K si values for the oligogalacturonides were in the low mM range, and K i for product galacturonic acid was in the low µM range. Kinetic modeling of the progress of reaction showed that the enzyme is both fully exo- and fully non-processional.
[Mh] Termos MeSH primário: Expressão Gênica
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/enzimologia
Poligalacturonase/genética
Poligalacturonase/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Domínio Catalítico
Clonagem Molecular
Sequência Conservada
Genes Sintéticos
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/química
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/metabolismo
Modelos Moleculares
Poligalacturonase/química
Multimerização Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 3.2.1.15 (Polygalacturonase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160523
[St] Status:MEDLINE
[do] DOI:10.1007/s12033-016-9948-8


  5 / 200 MEDLINE  
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[PMID]:26921187
[Au] Autor:Rizk M; Antranikian G; Elleuche S
[Ad] Endereço:Institute of Technical Microbiology, Hamburg University of Technology (TUHH), Kasernenstr. 12, 21073, Hamburg, Germany.
[Ti] Título:Influence of Linker Length Variations on the Biomass-Degrading Performance of Heat-Active Enzyme Chimeras.
[So] Source:Mol Biotechnol;58(4):268-79, 2016 Apr.
[Is] ISSN:1559-0305
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant cell walls are composed of complex polysaccharides such as cellulose and hemicellulose. In order to efficiently hydrolyze cellulose, the synergistic action of several cellulases is required. Some anaerobic cellulolytic bacteria form multienzyme complexes, namely cellulosomes, while other microorganisms produce a portfolio of diverse enzymes that work in synergistic fashion. Molecular biological methods can mimic such effects through the generation of artificial bi- or multifunctional fusion enzymes. Endoglucanase and ß-glucosidase from extremely thermophilic anaerobic bacteria Fervidobacterium gondwanense and Fervidobacterium islandicum, respectively, were fused end-to-end in an approach to optimize polysaccharide degradation. Both enzymes are optimally active at 90 °C and pH 6.0-7.0 representing excellent candidates for fusion experiments. The direct linkage of both enzymes led to an increased activity toward the substrate specific for ß-glucosidase, but to a decreased activity of endoglucanase. However, these enzyme chimeras were superior over 1:1 mixtures of individual enzymes, because combined activities resulted in a higher final product yield. Therefore, such fusion enzymes exhibit promising features for application in industrial bioethanol production processes.
[Mh] Termos MeSH primário: Celulase/metabolismo
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/enzimologia
beta-Glucosidase/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Biomassa
Catálise
Celulase/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Especificidade por Substrato
Temperatura Ambiente
beta-Glucosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Fusion Proteins); EC 3.2.1.21 (beta-Glucosidase); EC 3.2.1.4 (Cellulase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160228
[St] Status:MEDLINE
[do] DOI:10.1007/s12033-016-9925-2


  6 / 200 MEDLINE  
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[PMID]:26777430
[Au] Autor:Pyysalo MJ; Pyysalo LM; Pessi T; Karhunen PJ; Lehtimäki T; Oksala N; Öhman JE
[Ad] Endereço:a Department of Oral and Maxillofacial Diseases , Tampere University Hospital , Tampere , Finland ;
[Ti] Título:Bacterial DNA findings in ruptured and unruptured intracranial aneurysms.
[So] Source:Acta Odontol Scand;74(4):315-20, 2016.
[Is] ISSN:1502-3850
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Chronic inflammation has earlier been detected in ruptured intracranial aneurysms. A previous study detected both dental bacterial DNA and bacterial-driven inflammation in ruptured intracranial aneurysm walls. The aim of this study was to compare the presence of oral and pharyngeal bacterial DNA in ruptured and unruptured intracranial aneurysms. The hypothesis was that oral bacterial DNA findings would be more common and the amount of bacterial DNA would be higher in ruptured aneurysm walls than in unruptured aneurysm walls. MATERIALS AND METHODS: A total of 70 ruptured (n = 42) and unruptured (n = 28) intracranial aneurysm specimens were obtained perioperatively in aneurysm clipping operations. Aneurysmal sac tissue was analysed using a real-time quantitative polymerase chain reaction to detect bacterial DNA from several oral species. Both histologically non-atherosclerotic healthy vessel wall obtained from cardiac by-pass operations (LITA) and arterial blood samples obtained from each aneurysm patient were used as control samples. RESULTS: Bacterial DNA was detected in 49/70 (70%) of the specimens. A total of 29/42 (69%) of the ruptured and 20/28 (71%) of the unruptured aneurysm samples contained bacterial DNA of oral origin. Both ruptured and unruptured aneurysm tissue samples contained significantly more bacterial DNA than the LITA control samples (p-values 0.003 and 0.001, respectively). There was no significant difference in the amount of bacterial DNA between the ruptured and unruptured samples. CONCLUSION: Dental bacterial DNA can be found using a quantitative polymerase chain reaction in both ruptured and unruptured aneurysm walls, suggesting that bacterial DNA plays a role in the pathogenesis of cerebral aneurysms in general, rather than only in ruptured aneurysms.
[Mh] Termos MeSH primário: Aneurisma Roto/microbiologia
DNA Bacteriano/isolamento & purificação
Aneurisma Intracraniano/microbiologia
Boca/microbiologia
[Mh] Termos MeSH secundário: Aggregatibacter actinomycetemcomitans/genética
Feminino
Fusobacterium nucleatum/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Seres Humanos
Masculino
Meia-Idade
Peptostreptococcus/genética
Faringe/microbiologia
Porphyromonas gingivalis/genética
Prevotella intermedia/genética
Staphylococcus aureus/genética
Staphylococcus epidermidis/genética
Streptococcus anginosus/genética
Streptococcus gordonii/genética
Streptococcus mitis/genética
Streptococcus oralis/genética
Streptococcus sanguis/genética
Dente/microbiologia
Treponema denticola/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160119
[St] Status:MEDLINE
[do] DOI:10.3109/00016357.2015.1130854


  7 / 200 MEDLINE  
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[PMID]:26101016
[Au] Autor:Kanoksilapatham W; Keawram P; Gonzalez JM; Robb FT
[Ad] Endereço:Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand, kanoksilapatham_w@su.ac.th.
[Ti] Título:Isolation, characterization, and survival strategies of Thermotoga sp. strain PD524, a hyperthermophile from a hot spring in Northern Thailand.
[So] Source:Extremophiles;19(4):853-61, 2015 Jul.
[Is] ISSN:1433-4909
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A hyperthermophilic Thermotoga sp. strain PD524 was isolated from a hot spring in Northern Thailand. Cells were long-curved rods (0.5-0.6 × 2.5-10 µm) surrounded by a typical outer membrane toga. Strain PD524 is aero-tolerant at 4 °C but is aero-sensitive at 80 °C. A heat resistant subpopulation was observed in late-stationary phase. Cells from late-stationary phase were revealed remarkably less sensitive to 0.001 % SDS treatment than cells from exponential phase. The temperature range for growth was 70-85 °C (opt. temp. 80 °C), pH range was 6-8.5 (opt. pH 7.5-8.0), and NaCl range of 0 to <10 g/L (opt. 0.5 g/L). Glucose, sucrose, maltose, fructose, xylose, mannose, arabinose, trehalose, starch, and cellobiose were utilized as growth substrates. Growth was inhibited by S(o). Growth yield was stimulated by SO 4 (=) but not by S2O 3 (=) and NO3 (-). Analysis of 16S rRNA gene sequence (KF164213) of strain PD524 revealed closest similarity (96 %) to Thermotoga maritima MSB8(T), T. neapolitana NES(T), T. petrophila RKU-1(T), and T. naphthophila RKU-10(T).
[Mh] Termos MeSH primário: Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos
Fontes Termais/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/crescimento & desenvolvimento
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/isolamento & purificação
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:150624
[St] Status:MEDLINE
[do] DOI:10.1007/s00792-015-0761-2


  8 / 200 MEDLINE  
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[PMID]:25904141
[Au] Autor:Park OJ; Yi H; Jeon JH; Kang SS; Koo KT; Kum KY; Chun J; Yun CH; Han SH
[Ad] Endereço:Department of Oral Microbiology and Immunology, DRI and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul, Korea.
[Ti] Título:Pyrosequencing Analysis of Subgingival Microbiota in Distinct Periodontal Conditions.
[So] Source:J Dent Res;94(7):921-7, 2015 Jul.
[Is] ISSN:1544-0591
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Subgingival microorganisms are potentially associated with periodontal diseases. However, changes in the subgingival microbiota during the progress of periodontal diseases are poorly understood. In this study, we analyzed bacterial communities in the subgingival paper point samples from 32 Korean individuals with no sign of disease, gingivitis, or periodontitis using 454 FLX Titanium pyrosequencing. A total of 256,113 reads representing 26 phyla, 433 genera, and 1,016 species were detected. Bacteroidetes, Fusobacteria, Synergistetes, and Spirochaetes were the abundant phyla in periodontitis subjects, whereas Firmicutes and Proteobacteria were identified as the dominant phyla in the gingivitis and healthy subjects, respectively. Although high levels of Porphyromonas, Fusobacterium, Fretibacterium, Rothia, Filifactor, and Treponema genera were observed in the periodontitis subjects, Streptococcus, Capnocytophaga, Leptotrichia, and Haemophilus genera were found at high frequency in the gingivitis subjects. Species including Porphyromonas gingivalis, Fusobacterium nucleatum, and Fretibacterium fastidiosum were significantly increased in periodontitis subjects. On the other hand, Streptococcus pseudopneumoniae, Haemophilus parainfluenzae, and Leptotrichia hongkongensis were preferentially observed in the gingivitis subjects. Intriguingly, the halophile Halomonas hamiltonii was revealed as a predominant species in the healthy subjects. Based on Fast UniFrac analysis, distinctive bacterial clusters were classified for the healthy, gingivitis, and periodontitis state. The current findings might be useful for understanding the pathogenesis, diagnosis, and treatment of periodontal diseases.
[Mh] Termos MeSH primário: Bactérias/classificação
Gengivite/microbiologia
Periodontite/microbiologia
Periodonto/microbiologia
[Mh] Termos MeSH secundário: Actinomycetaceae/classificação
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Bacteroidetes/isolamento & purificação
Capnocytophaga/isolamento & purificação
Fusobactérias/isolamento & purificação
Fusobacterium/classificação
Fusobacterium nucleatum/isolamento & purificação
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/classificação
Bactérias Gram-Negativas/classificação
Haemophilus/classificação
Haemophilus parainfluenzae/isolamento & purificação
Halomonas/isolamento & purificação
Seres Humanos
Leptotrichia/isolamento & purificação
Meia-Idade
Porphyromonas/classificação
Porphyromonas gingivalis/isolamento & purificação
Proteobactérias/isolamento & purificação
Análise de Sequência de DNA
Spirochaetales/isolamento & purificação
Streptococcus/classificação
Treponema/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150623
[Lr] Data última revisão:
150623
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:150424
[St] Status:MEDLINE
[do] DOI:10.1177/0022034515583531


  9 / 200 MEDLINE  
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[PMID]:25838236
[Au] Autor:Xie J; Zhao D; Zhao L; Pei J; Xiao W; Ding G; Wang Z
[Ad] Endereço:College of Chemical Engineering, Nanjing Forestry University, 159 Long Pan Road, Nanjing, 210037, China.
[Ti] Título:Overexpression and characterization of a Ca(2+) activated thermostable ß-glucosidase with high ginsenoside Rb1 to ginsenoside 20(S)-Rg3 bioconversion productivity.
[So] Source:J Ind Microbiol Biotechnol;42(6):839-50, 2015 Jun.
[Is] ISSN:1476-5535
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The thermostable ß-glucosidase gene from Thermotoga petrophila DSM 13995 was cloned and overexpressed in Escherichia coli. The activity of the recombinant ß-glucosidase was 21 U/mL in the LB medium. Recombinant ß-glucosidase was purified, and its molecular weight was approximately 81 kDa. The optimal activity was at pH 5.0 and 90 °C, and the thermostability of the enzyme was improved by Ca(2+). The ß-glucosidase had high selectivity for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produced the pharmacologically active minor ginsenoside 20(S)-Rg3. In a reaction at 90 °C and pH 5.0, 10 g/L of ginsenoside Rb1 was transformed into 6.93 g/L of Rg3 within 90 min, with a corresponding molar conversion of 97.9%, and Rg3 productivity of 4620 mg/L/h. This study is the first report of a GH3-family enzyme that used Ca(2+) to improve its thermostability, and it is the first report on the high substrate concentration bioconversion of ginsenoside Rb1 to ginsenoside 20(S)-Rg3 by using thermostable ß-glucosidase under high temperature.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Ginsenosídeos/metabolismo
beta-Glucosidase/genética
beta-Glucosidase/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Cálcio/farmacologia
Ativação Enzimática
Estabilidade Enzimática/efeitos dos fármacos
Escherichia coli/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/enzimologia
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Concentração de Íons de Hidrogênio
Peso Molecular
Temperatura Ambiente
beta-Glucosidase/química
beta-Glucosidase/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ginsenosides); 7413S0WMH6 (ginsenoside Rb1); EC 3.2.1.21 (beta-Glucosidase); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150404
[St] Status:MEDLINE
[do] DOI:10.1007/s10295-015-1608-7


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[PMID]:25736413
[Au] Autor:Zeng X; Zhang Z; Li X; Jebbar M; Alain K; Shao Z
[Ad] Endereço:1Key Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography SOA, Xiamen, Fujian 361005, PR China 3Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, Fujian 361005, PR China 2Collaborative Innovation Center of Deep Sea Biology, Xiamen, Fujian 361005, PR Chi
[Ti] Título:Caloranaerobacter ferrireducens sp. nov., an anaerobic, thermophilic, iron (III)-reducing bacterium isolated from deep-sea hydrothermal sulfide deposits.
[So] Source:Int J Syst Evol Microbiol;65(Pt 6):1714-8, 2015 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A thermophilic, anaerobic, iron-reducing bacterium (strain DY22619T) was isolated from a sulfide sample collected from an East Pacific Ocean hydrothermal field at a depth of 2901 m. Cells were Gram-stain-negative, motile rods (2-10 µm in length, 0.5 µm in width) with multiple peritrichous flagella. The strain grew at 40-70 °C inclusive (optimum 60 °C), at pH 4.5-8.5 inclusive (optimum pH 7.0) and with sea salts concentrations of 1-10 % (w/v) (optimum 3 % sea salts) and NaCl concentrations of 1.5-5.0 % (w/v) (optimum 2.5 % NaCl). Under optimal growth conditions, the generation time was around 55 min. The isolate was an obligate chemoorganoheterotroph, utilizing complex organic compounds, amino acids, carbohydrates and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamate, methionine, threonine, fructose, mannose, galactose, glucose, palatinose, rhamnose, turanose, gentiobiose, xylose, sorbose, pyruvate, tartaric acid, α-ketobutyric acid, α-ketovaleric acid, galacturonic acid and glucosaminic acid. Strain DY22619T was strictly anaerobic and facultatively dependent on various forms of Fe(III) as an electron acceptor: insoluble forms and soluble forms. It did not reduce sulfite, sulfate, thiosulfate or nitrate. The genomic DNA G+C content was 29.0 mol%. Phylogenetic 16S rRNA gene sequence analyses revealed that the closest relative of strain DY22619T was Caloranaerobacter azorensis MV1087T, sharing 97.41 % 16S rRNA gene sequence similarity. On the basis of physiological distinctness and phylogenetic distance, the isolate is considered to represent a novel species of the genus Caloranaerobacter, for which the name Caloranaerobacterhttp://dx.doi.org/10.1601/nm.4081ferrireducens sp. nov. is proposed. The type strain is DY22619T ( = JCM 19467T = DSM 27799T = MCCC1A06455T).
[Mh] Termos MeSH primário: Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/classificação
Fontes Hidrotermais/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Compostos Férricos/metabolismo
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética
Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/isolamento & purificação
Ferro
Dados de Sequência Molecular
Oceano Pacífico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Sulfetos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Ferric Compounds); 0 (RNA, Ribosomal, 16S); 0 (Sulfides); E1UOL152H7 (Iron)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150828
[Lr] Data última revisão:
150828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150305
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.000165



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