Base de dados : MEDLINE Pesquisa : B03.440.425.410.360 [Categoria DeCS] Referências encontradas : 26 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 26039558 [Au] Autor: Alves A; Ly HK; Hildebrandt P; Louro RO; Millo D [Ad] Endereço: †Instituto de Tecnologia QuiÌ�mica e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República-Estação Agronómica Nacional, 2780-157 Oeiras, Portugal. [Ti] Título: Nature of the Surface-Exposed Cytochrome-Electrode Interactions in Electroactive Biofilms of Desulfuromonas acetoxidans. [So] Source: J Phys Chem B;119(25):7968-74, 2015 Jun 25. [Is] ISSN: 1520-5207 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Metal-respiring bacteria are microorganisms capable of oxidizing organic pollutants present in wastewater and transferring the liberated electrons to an electrode. This ability has led to their application as catalysts in bioelectrochemical systems (BESs), a sustainable technology coupling bioremediation to electricity production. Crucial for the functioning of these BESs is a complex protein architecture consisting of several surface-exposed multiheme proteins, called outer membrane cytochromes, wiring the cell metabolism to the electrode. Although the role of these proteins has been increasingly understood, little is known about the protein-electrode interactions and their impact on the performance of BESs. In this study, we used surface-enhanced resonance Raman spectroscopy in combination with electrochemical techniques to unravel the nature of the protein-electrode interaction for the outer membrane cytochrome OmcB from Desulfuromonas acetoxidans (Dace). Comparing the spectroelectrochemical properties of OmcB bound directly to the electrode surface with those of the same protein embedded inside an electroactive biofilm, we have shown that the surface-exposed cytochromes of Dace biofilms are in direct contact with the electrode surface. Even if direct binding causes protein denaturation, the biofilm possesses the ability to minimize the extent of the damage maximizing the amount of cells in direct electrical communication with the electrode. [Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/metabolismo Fontes de Energia Bioelétrica Biofilmes Citocromos/metabolismo Desulfuromonas/fisiologia Eletrodos [Mh] Termos MeSH secundário: Análise Espectral Raman [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Bacterial Outer Membrane Proteins); 0 (Cytochromes); 0 (OmcB protein, bacteria) [Em] Mês de entrada: 1603 [Cu] Atualização por classe: 150625 [Lr] Data última revisão: 150625 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150604 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jpcb.5b03419

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[PMID]: 25736408 [Au] Autor: An TT; Picardal FW [Ad] Endereço: School of Public and Environmental Affairs, Indiana University, MSB II, 702 N. Walnut Grove Ave, Bloomington, IN 47405-2204, USA. [Ti] Título: Desulfuromonas carbonis sp. nov., an Fe(III)-, S0- and Mn(IV)-reducing bacterium isolated from an active coalbed methane gas well. [So] Source: Int J Syst Evol Microbiol;65(Pt 5):1686-93, 2015 May. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A novel, mesophilic, obligately anaerobic, acetate-oxidizing, dissimilatory iron-, sulfur-, and manganese-reducing bacterium, designated strain ICBM(T), was obtained from an active, coalbed methane gas well in Indiana, USA. Strain ICBM(T) was a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium that was rich in c-type cytochromes and formed red colonies in solid medium. Strain ICBM(T) conserved energy to support growth from the oxidation of acetate, propionate, pyruvate, malate, fumarate, succinate and dl-lactate, concomitant with dissimilatory iron reduction. Strain ICBM(T) fermented fumarate yielding succinate and acetate. Strain ICBM(T) was able to grow in the temperature range of 10 °C to 37 °C, NaCl concentration range of 0 to 1.2 M, and pH range of 6.5 to 8.0. The physiological characteristics of strain ICBM(T) indicated that it belongs to the Desulfuromonas cluster. The G+C content of its genomic DNA was 61.2 mol%. The predominant cellular fatty acids were C16 : 0 (39.3%), C16 : 1ω7c and/or iso-C15 : 0 2-OH (36.6%). The closest cultured phylogenetic relative of strain ICBM(T) was Desulfuromonas michiganensis BB1(T) with only 95% 16S rRNA gene sequence similarity. This confirmed that strain ICBM(T) is affiliated with the genus Desulfuromonas . On the basis of phenotypic and genotypic differences between strain ICBM(T) and other taxa of the genus Desulfuromonas , strain ICBM(T) represents a novel species for which the name Desulfuromonas carbonis sp. nov. is proposed (type strain ICBM(T) = DSM 29759(T) = JCM 30471(T)). Strain ICBM(T) is the first Fe(III)-, S(0)-, and Mn(IV)-reducing bacterium that was isolated from a coal bed. [Mh] Termos MeSH primário: Desulfuromonas/classificação Campos de Petróleo e Gás/microbiologia Filogenia [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases Citocromos/química DNA Bacteriano/genética Desulfuromonas/genética Desulfuromonas/isolamento & purificação Ácidos Graxos/química Compostos Férricos/metabolismo Indiana Compostos de Manganês/metabolismo Metano Dados de Sequência Molecular Oxirredução RNA Ribossômico 16S/genética Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cytochromes); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Ferric Compounds); 0 (Manganese Compounds); 0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane) [Em] Mês de entrada: 1510 [Cu] Atualização por classe: 150617 [Lr] Data última revisão: 150617 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150305 [St] Status: MEDLINE [do] DOI: 10.1099/ijs.0.000159

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[PMID]: 25734617 [Au] Autor: Osborne TH; McArthur JM; Sikdar PK; Santini JM [Ad] Endereço: †Institute of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, United Kingdom. [Ti] Título: Isolation of an arsenate-respiring bacterium from a redox front in an arsenic-polluted aquifer in West Bengal, Bengal Basin. [So] Source: Environ Sci Technol;49(7):4193-9, 2015 Apr 07. [Is] ISSN: 1520-5851 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Natural pollution of groundwater by arsenic adversely affects the health of tens of millions of people worldwide, with the deltaic aquifers of SE Asia being particularly polluted. The pollution is caused primarily by, or as a side reaction of, the microbial reduction of sedimentary Fe(III)-oxyhydroxides, but the organism(s) responsible for As release have not been isolated. Here we report the first isolation of a dissimilatory arsenate reducer from sediments of the Bengal Basin in West Bengal. The bacterium, here designated WB3, respires soluble arsenate and couples its reduction to the oxidation of acetate; WB3 is therefore implicated in the process of arsenic pollution of groundwater, which is largely by arsenite. The bacterium WB3 is also capable of reducing dissolved Fe(III) citrate, solid Fe(III)-oxyhydroxide, and elemental sulfur, using acetate as the electron donor. It is a member of the Desulfuromonas genus and possesses a dissimilatory arsenate reductase that was identified using degenerate polymerase chain reaction primers. The sediment from which WB3 was isolated was brown, Pleistocene sand at a depth of 35.2 m below ground level (mbgl). This level was some 3 cm below the boundary between the brown sands and overlying reduced, gray, Holocene aquifer sands. The color boundary is interpreted to be a reduction front that releases As for resorption downflow, yielding a high load of labile As sorbed to the sediment at a depth of 35.8 mbgl and concentrations of As in groundwater that reach >1000 µg/L. [Mh] Termos MeSH primário: Arseniatos/química Arsênico/análise Desulfuromonas/isolamento & purificação Monitoramento Ambiental/métodos Água Subterrânea/microbiologia Poluentes Químicos da Água/análise [Mh] Termos MeSH secundário: Arsênico/química Ásia Ocidental Desulfuromonas/crescimento & desenvolvimento Compostos Férricos/análise Sedimentos Geológicos/química Sedimentos Geológicos/microbiologia Oxirredução Poluentes Químicos da Água/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Arsenates); 0 (Ferric Compounds); 0 (Water Pollutants, Chemical); N712M78A8G (Arsenic); N7CIZ75ZPN (arsenic acid) [Em] Mês de entrada: 1601 [Cu] Atualização por classe: 150408 [Lr] Data última revisão: 150408 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150304 [St] Status: MEDLINE [do] DOI: 10.1021/es504707x

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[PMID]: 25717030 [Au] Autor: Pierra M; Carmona-Martínez AA; Trably E; Godon JJ; Bernet N [Ad] Endereço: INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, F-11100 Narbonne, France. [Ti] Título: Specific and efficient electrochemical selection of Geoalkalibacter subterraneus and Desulfuromonas acetoxidans in high current-producing biofilms. [So] Source: Bioelectrochemistry;106(Pt A):221-5, 2015 Dec. [Is] ISSN: 1878-562X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Two different saline sediments were used to inoculate potentiostatically controlled reactors (a type of microbial bioelectrochemical system, BES) operated in saline conditions (35 gNaCl l(-1)). Reactors were fed with acetate or a mixture of acetate and butyrate at two pH values: 7.0 or 5.5. Electroactive biofilm formation lag-phase, maximum current density production and coulombic efficiency were used to evaluate the overall performance of reactors. High current densities up to 8.5 A m(-2) were obtained using well-defined planar graphite electrodes. Additionally, biofilm microbial communities were characterized by CE-SSCP and 454 pyrosequencing. As a result of this procedure, two anode-respiring bacteria (ARB) always dominated the anodic biofilms: Geoalkalibacter subterraneus and/or Desulfuromonas acetoxidans. This suggests that a strong electrochemically driven selection process imposed by the applied potential occurs in the BES system. Moreover, the emergence of Glk. subterraneus in anodic biofilms significantly contributes to broaden the spectrum of high current producing microorganisms electrochemically isolated from environmental samples. [Mh] Termos MeSH primário: Fontes de Energia Bioelétrica/microbiologia Biofilmes Desulfuromonas/metabolismo Condutividade Elétrica [Mh] Termos MeSH secundário: Acetatos/metabolismo Biofilmes/crescimento & desenvolvimento Ácido Butírico/metabolismo Desulfuromonas/fisiologia Eletroquímica Transporte de Elétrons Concentração de Íons de Hidrogênio [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Acetates); 107-92-6 (Butyric Acid) [Em] Mês de entrada: 1606 [Cu] Atualização por classe: 150911 [Lr] Data última revisão: 150911 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150227 [St] Status: MEDLINE

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[PMID]: 24868908 [Au] Autor: Samarukha IA [Ti] Título: [Mechanisms of electron transfer to insoluble terminal acceptors in chemoorganotrophic bacteria]. [So] Source: Ukr Biochem J;86(2):16-25, 2014 Mar-Apr. [Is] ISSN: 2409-4943 [Cp] País de publicação: Ukraine [La] Idioma: ukr [Ab] Resumo: The mechanisms of electron transfer of association of chemoorganotrophic bacteria to the anode in microbial fuel cells are summarized in the survey. These mechanisms are not mutually exclusive and are divided into the mechanisms of mediator electron transfer, mechanisms of electron transfer with intermediate products of bacterial metabolism and mechanism of direct transfer of electrons from the cell surface. Thus, electron transfer mediators are artificial or synthesized by bacteria riboflavins and phenazine derivatives, which also determine the ability of bacteria to antagonism. The microorganisms with hydrolytic and exoelectrogenic activity are involved in electron transfer mechanisms that are mediated by intermediate metabolic products, which are low molecular carboxylic acids, alcohols, hydrogen etc. The direct transfer of electrons to insoluble anode is possible due to membrane structures (cytochromes, pili, etc.). Association of microorganisms, and thus the biochemical mechanisms of electron transfer depend on the origin of the inoculum, substrate composition, mass transfer, conditions of aeration, potentials and location of electrodes and others, that are defined by technological and design parameters. [Mh] Termos MeSH primário: Proteínas de Bactérias/química Desulfovibrio/química Desulfuromonas/química Elétrons Geobacter/química Shewanella/química [Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo Fontes de Energia Bioelétrica/utilização Desulfovibrio/metabolismo Desulfuromonas/metabolismo Eletrodos Transporte de Elétrons Geobacter/metabolismo Oxirredução Shewanella/metabolismo [Pt] Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Bacterial Proteins) [Em] Mês de entrada: 1406 [Cu] Atualização por classe: 150504 [Lr] Data última revisão: 150504 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140530 [St] Status: MEDLINE

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[PMID]: 24279627 [Au] Autor: Kim IS; Ekpeghere KI; Ha SY; Kim BS; Song B; Kim JT; Kim HG; Koh SC [Ad] Endereço: a Department of Environmental Engineering , Korea Maritime University , Busan , Republic of Korea. [Ti] Título: Full-scale biological treatment of tannery wastewater using the novel microbial consortium BM-S-1. [So] Source: J Environ Sci Health A Tox Hazard Subst Environ Eng;49(3):355-64, 2014. [Is] ISSN: 1532-4117 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: In order to develop a more effective and eco-friendly treatment technology, a full-scale tannery wastewater treatment plant with a sludge digestion system was augmented with a novel microbial consortium (BM-S-1). The aim of this study was to determine if the BM-S-1 could successfully treat the tannery wastewater in a full-scale treatment system without chemical pretreatment and to investigate effect of the augmentation on sludge production. Chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), chromium (Cr) and mixed liquor suspended solids (MLSS) were measured to monitor treated water quality and treatment efficiency. Microbial community structures in the treatment were also examined using pyrosequencing analysis of 16S rRNA gene and quantitative PCR (qPCR) of the nitrous oxide reductase gene (nosZ). The removal efficiencies of COD, TN, TP, and Cr were estimated to be 98.3%, 98.6%, 93.6%, and 88.5%, respectively, while the system without a continuous augmentation was broken down. The pyrosequencing analysis showed Brachymonas denitrificans to be the most dominant microbial population in the buffering tank (B; 37.5%). Potential polymeric substance degraders (Clostridia), sulfate reducers (Desulfuromonas palmitatis), and sulfur oxidizers (uncultured Thiobacillus) were dominant in the sludge digestion (SD) tank. The denitrifiers assayed by nosZ qPCR were dominant in B and SD. These microbial communities appeared to play important roles in removing nutrients and odor, and reducing sludge in the wastewater treatment plant without chemical pretreatment. [Mh] Termos MeSH primário: Consórcios Microbianos Eliminação de Resíduos Líquidos/métodos Águas Residuais [Mh] Termos MeSH secundário: Análise da Demanda Biológica de Oxigênio Cromo/metabolismo Clostridium/metabolismo Desulfuromonas Resíduos Industriais Consórcios Microbianos/genética Nitrogênio/metabolismo Fósforo/metabolismo Esgotos Qualidade da Água [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Industrial Waste); 0 (Sewage); 0 (Waste Water); 0R0008Q3JB (Chromium); 27YLU75U4W (Phosphorus); N762921K75 (Nitrogen) [Em] Mês de entrada: 1408 [Cu] Atualização por classe: 160526 [Lr] Data última revisão: 160526 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 131128 [St] Status: MEDLINE [do] DOI: 10.1080/10934529.2014.846707

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[PMID]: 24169815 [Au] Autor: Zhang T; Bain TS; Barlett MA; Dar SA; Snoeyenbos-West OL; Nevin KP; Lovley DR [Ad] Endereço: Department of Microbiology, University of Massachusetts, Amherst, MA, USA. [Ti] Título: Sulfur oxidation to sulfate coupled with electron transfer to electrodes by Desulfuromonas strain TZ1. [So] Source: Microbiology;160(Pt 1):123-9, 2014 Jan. [Is] ISSN: 1465-2080 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Microbial oxidation of elemental sulfur with an electrode serving as the electron acceptor is of interest because this may play an important role in the recovery of electrons from sulfidic wastes and for current production in marine benthic microbial fuel cells. Enrichments initiated with a marine sediment inoculum, with elemental sulfur as the electron donor and a positively poised (+300 mV versus Ag/AgCl) anode as the electron acceptor, yielded an anode biofilm with a diversity of micro-organisms, including Thiobacillus, Sulfurimonas, Pseudomonas, Clostridium and Desulfuromonas species. Further enrichment of the anode biofilm inoculum in medium with elemental sulfur as the electron donor and Fe(III) oxide as the electron acceptor, followed by isolation in solidified sulfur/Fe(III) medium yielded a strain of Desulfuromonas, designated strain TZ1. Strain TZ1 effectively oxidized elemental sulfur to sulfate with an anode serving as the sole electron acceptor, at rates faster than Desulfobulbus propionicus, the only other organism in pure culture previously shown to oxidize S° with current production. The abundance of Desulfuromonas species enriched on the anodes of marine benthic fuel cells has previously been interpreted as acetate oxidation driving current production, but the results presented here suggest that sulfur-driven current production is a likely alternative. [Mh] Termos MeSH primário: Desulfuromonas/metabolismo Eletricidade Eletrodos/microbiologia Sulfatos/metabolismo Enxofre/metabolismo [Mh] Termos MeSH secundário: Fontes de Energia Bioelétrica DNA Bacteriano/química DNA Bacteriano/genética Desulfuromonas/classificação Desulfuromonas/genética Desulfuromonas/isolamento & purificação Sedimentos Geológicos/microbiologia Dados de Sequência Molecular Oxirredução Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Sulfates); 70FD1KFU70 (Sulfur) [Em] Mês de entrada: 1408 [Cu] Atualização por classe: 140103 [Lr] Data última revisão: 140103 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 131031 [St] Status: MEDLINE [do] DOI: 10.1099/mic.0.069930-0

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[PMID]: 23963272 [Au] Autor: Huang D; Lai Y; Becker JG [Ad] Endereço: HydroGeoLogic Inc, Reston, VA, 20190, USA. [Ti] Título: Impact of initial conditions on extant microbial kinetic parameter estimates: application to chlorinated ethene dehalorespiration. [So] Source: Appl Microbiol Biotechnol;98(5):2279-88, 2014 Mar. [Is] ISSN: 1432-0614 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Monod kinetics are the foundation of mathematical models of many environmentally important biological processes, including the dehalorespiration of chlorinated ethene groundwater contaminants. The Monod parameters--qmax, the maximum specific substrate utilization rate, and KS, the half-saturation constant--are typically estimated in batch assays, which are superficially simple to prepare and maintain. However, if initial conditions in batch assays are not chosen carefully, it is unlikely that the estimated parameter values will be meaningful because they do not reflect microbial activity in the environmental system of interest, and/or they are not mathematically identifiable. The estimation of qmax and KS values that are highly correlated undoubtedly contributes significantly to the wide range in reported parameter values and may undermine efforts to use mathematical models to demonstrate the occurrence of natural attenuation or predict the performance of engineered bioremediation approaches. In this study, a series of experimental and theoretical batch kinetic assays were conducted using the tetrachloroethene-respirer Desulfuromonas michiganensis to systematically evaluate the effects of initial batch assay conditions, expressed as the initial substrate (S0)-to-initial biomass concentration (X0) ratio (S0/X0) and the S0/KS ratio on parameter correlation. An iterative approach to obtain meaningful Monod parameter estimates was developed and validated using three different strains and can be broadly applied to a range of other substrates and populations. While the S0/X0 ratio is critical to obtaining kinetic parameter estimates that reflect in situ microbial activity, this study shows that optimization of the S0/KS ratio is key to minimizing Monod parameter correlation. [Mh] Termos MeSH primário: Desulfuromonas/metabolismo Tetracloroetileno/metabolismo [Mh] Termos MeSH secundário: Modelos Teóricos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Nm] Nome de substância: TJ904HH8SN (Tetrachloroethylene) [Em] Mês de entrada: 1410 [Cu] Atualização por classe: 140224 [Lr] Data última revisão: 140224 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130822 [St] Status: MEDLINE [do] DOI: 10.1007/s00253-013-5171-8

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[PMID]: 23720962 [Au] Autor: Vasyliv OM; Hnatush SO [Ti] Título: [Influence of transition metal compounds on superoxide dismutase activity of sulfur reducing Desulfuromonas acetoxidans bacteria]. [So] Source: Mikrobiol Z;75(2):37-44, 2013 Mar-Apr. [Is] ISSN: 1028-0987 [Cp] País de publicação: Ukraine [La] Idioma: ukr [Ab] Resumo: Superoxide dismutase, as one of the enzymes of cells' antioxidant defensive system, catalyzes superoxide anion-radical (O2-) dismutation with O2 and H2O2 forming. The influence of such transition metal compounds, as FeSO4, FeCl3, MnCl2, NiCl2, and CoCl2 on superoxide dismutase activity of sulfur-reducing Desulfuromonas acetoxidans bacteria has been investigated. Maximal activity of the investigated enzyme has been observed accordingly under the influence of 1.0 mM of NiCl2, 2.0 mM of CoCl2 and MnCl2 on the second day and under the influence of 1.0 mM of FeCl3 and FeSO4 respectively, on the third day of growth in comparison with control samples. An increase of incubation time and concentration of metal compound in the medium caused the inhibition of superoxide dismutase activity. [Mh] Termos MeSH primário: Proteínas de Bactérias/antagonistas & inibidores Desulfuromonas/efeitos dos fármacos Desulfuromonas/enzimologia Superóxido Dismutase/antagonistas & inibidores Elementos de Transição/farmacologia [Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo Cloretos/farmacologia Cobalto/farmacologia Meios de Cultura Desulfuromonas/crescimento & desenvolvimento Relação Dose-Resposta a Droga Ensaios Enzimáticos Compostos Férricos/farmacologia Compostos Ferrosos/farmacologia Cinética Compostos de Manganês/farmacologia Níquel/farmacologia Oxirredução/efeitos dos fármacos Superóxido Dismutase/metabolismo [Pt] Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Chlorides); 0 (Culture Media); 0 (Ferric Compounds); 0 (Ferrous Compounds); 0 (Manganese Compounds); 0 (Transition Elements); 39R4TAN1VT (ferrous sulfate); 3G0H8C9362 (Cobalt); 696BNE976J (nickel chloride); 7OV03QG267 (Nickel); EC 1.15.1.1 (Superoxide Dismutase); EVS87XF13W (cobaltous chloride); QQE170PANO (manganese chloride); U38V3ZVV3V (ferric chloride) [Em] Mês de entrada: 1306 [Cu] Atualização por classe: 151119 [Lr] Data última revisão: 151119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130601 [St] Status: MEDLINE

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[PMID]: 23143172 [Au] Autor: Cheng G; Hu Y; Lu N; Li J; Wang Z; Chen Q; Zhu B [Ad] Endereço: Microbial Genome Research Center, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China. chengg@im.ac.cn [Ti] Título: Identification of a novel fosfomycin-resistant UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from a soil metagenome. [So] Source: Biotechnol Lett;35(2):273-8, 2013 Feb. [Is] ISSN: 1573-6776 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A soil metagenomic library was constructed and screened for clones that conferred fosfomycin resistance. A novel protein with 46 % identity to UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Desulfuromonas acetoxidans DSM 684 (GenBank accession number: ZP_01311756) was identified. Multiple sequence alignment revealed that the novel protein was a natural MurA, in which an aspartic acid instead of a cysteine was located in the active site. An Asp120Cys mutant of Escherichia coli was constructed from the subclone through site-specific mutagenesis, and minimum inhibitory concentration of fosfomycin for the resistant subclone and its mutant were determined. These results showed that fosfomycin resistance was a result of the aspartic acid in the active site. Analysis of all existing MurA sequences revealed that MurAs with an active site aspartic acid that can confer fosfomycin resistance occur in ~14 % of bacteria. [Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo Antibacterianos/farmacologia Farmacorresistência Bacteriana Escherichia coli/efeitos dos fármacos Fosfomicina/farmacologia Metagenoma Microbiologia do Solo [Mh] Termos MeSH secundário: Alquil e Aril Transferases/genética Alquil e Aril Transferases/isolamento & purificação Domínio Catalítico/genética Desulfuromonas/genética Escherichia coli/genética Testes de Sensibilidade Microbiana Mutagênese Sítio-Dirigida Proteínas Mutantes/genética Proteínas Mutantes/metabolismo Homologia de Sequência de Aminoácidos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Mutant Proteins); 2N81MY12TE (Fosfomycin); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.7 (UDP-N-acetylglucosamine 1-carboxyvinyltransferase) [Em] Mês de entrada: 1307 [Cu] Atualização por classe: 131121 [Lr] Data última revisão: 131121 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 121113 [St] Status: MEDLINE [do] DOI: 10.1007/s10529-012-1074-5

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2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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