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  1 / 93 MEDLINE  
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[PMID]:28895523
[Au] Autor:Shivani Y; Subhash Y; Sasikala C; Ramana CV
[Ad] Endereço:1​Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
[Ti] Título:Characterisation of a newly isolated member of a candidatus lineage, Marispirochaeta aestuarii gen. nov., sp. nov.
[So] Source:Int J Syst Evol Microbiol;67(10):3929-3936, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Metagenome analysis of coastal marine habitats of Gujarat, India indicated the presence of twelve novel putative lineages of spirochaetes. Out of which a strain designated JC444 representing a novel putative lineage seven was isolated and characterized based on a polyphasic taxonomic approach. Strain JC444 was helical, Gram-stain-negative, obligate anaerobe, catalase and oxidase negative. Strain JC444 was able to grow at 15-45 °C (optimum at 30-35 °C), pH 6.5-8.6 (optimum at 7.5-8.0) and 0.6-5 % (optimum at 1.5-2.0 %) of NaCl concentration. The major end products of glucose fermentation were acetate, formate, hydrogen and carbon dioxide. C14 : 0, iso-C15 : 0, C16 : 0, C18 : 0, iso-C15 : 1H/C13 : 03OH (summed feature 1), iso-C13 : 0, anteiso-C15 : 0 and iso-C17 : 0 were present as fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified lipids (L1-4) were the polar lipids. G+C mol% of strain JC444 was 53.6 %. 16S rRNA gene sequence comparisons indicated that strain JC444 represents a member of the family Spirochaetaceae in the order Spirochaetales. Strain JC444 has a sequence similarity of 97.1 % with 'Candidatus Marispirochaeta associata' JC231 and <90.1 % with other members of the family Spirochaetaceae. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC444 as a representative of a new genus and species in the family Spirochaetaceae, for which the name Marispirochaeta aestuarii gen. nov., sp. nov. is proposed. Type strain is JC444 (=KCTC 15554 =DSM 103365 ).
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Filogenia
Água do Mar/microbiologia
Spirochaetaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Spirochaetaceae/genética
Spirochaetaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002224


  2 / 93 MEDLINE  
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[PMID]:28875893
[Au] Autor:Subhash Y; Lee SS
[Ad] Endereço:Department of Life Science, College of Natural Science, Kyonggi University, 154-42 Gwanggyosan-ro Yeongtong-gu Suwon-Si, Gyeonggi-Do 16227, Republic of Korea.
[Ti] Título:Description of Oceanispirochaeta sediminicola gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov.
[So] Source:Int J Syst Evol Microbiol;67(9):3403-3409, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An obligately anaerobic spirochaete (strain SY2T) was isolated from coastal marine sediments of Tongyeong-Si, South Korea. Strain SY2T was helical-shaped and Gram-stain-negative. Strain SY2T was able to grow at 10-40 °C (optima, 25-30 °C), pH 6.3-8.8 (optima, pH 7.0-8.0) and with 1-7 % (optimum, 2-3 %) NaCl concentration. Strain SY2T was negative for catalase and oxidase activity. The major end-products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. C14 : 0, C16 : 0, iso-C15 : 0, iso-C14 : 0 3-OH, iso-C15 : 1 H/C13 : 0 3-OH and iso-C17 : 1ω9c were predominant fatty acids (>5 %) with minor amounts (<5 %) of C18 : 0, iso-C13 : 0, iso-C17 : 0, iso-C17 : 1/anteiso-C17 : 1 B and C16 : 1ω6c/C16 : 1ω7c. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic DNA G+C content was 53.5 mol%. 16S rRNA gene sequence comparisons indicated that strain SY2T represents a member of the family Spirochaetaceae in the phylum Spirochaetes. Strain SY2T has a sequence similarity of 95.1 % with Spirochaeta litoralis R1T and <90.1 % with other members of the genus Spirochaeta. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain SY2T as a representative of a novel genus and species in the family Spirochaetaceae, for which the name Oceanispirochaeta sediminicola gen. nov., sp. nov. is proposed. The type strain is SY2T (=KEMB 3001-381T=DSM 104770T=KCTC 15593T). Reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov. is also proposed based on polyphasic taxonomic analyses.
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Filogenia
Água do Mar/microbiologia
Spirochaetaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Spirochaetaceae/genética
Spirochaetaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 39382-08-6 (phosphatidylethanolamine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002130


  3 / 93 MEDLINE  
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[PMID]:28175253
[Au] Autor:Wessels W; Sprungala S; Watson SA; Miller DJ; Bourne DG
[Ad] Endereço:ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD 4811, Australia.
[Ti] Título:The microbiome of the octocoral Lobophytum pauciflorum: minor differences between sexes and resilience to short-term stress.
[So] Source:FEMS Microbiol Ecol;93(5), 2017 May 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacteria associated with marine invertebrates are thought to have a range of important roles that benefit the host including production of compounds that may exclude pathogenic microorganisms and recycling of essential nutrients. This study characterised the microbiome of a gonochoric octocoral, Lobophytum pauciflorum, and investigated whether either sex or environmental stresses influenced the diversity of the associated microbiome through amplicon profiling of the bacterial 16S rRNA gene. Sequences affiliated to Spirochaetaceae and Endozoicimonaceae dominated the microbiome of L. pauciflorum, representing 43% and 21% of the community, respectively. Among the dominant class affiliations, no sex-specific differences were detected, though unassigned sequences were at a 2-fold higher relative abundance in samples from female individuals than from males. These potentially novel sequences contributed to observed differences between sexes as detected by a multivariate analysis at the OTU level. Exposing L. pauciflorum fragments to increased temperature (31°C), decreased pH (7.9) or both stressors simultaneously for 12 days did not significantly alter the microbial community, indicating that the soft coral microbiome is relatively resilient to short-term environmental stress.
[Mh] Termos MeSH primário: Alphaproteobacteria/isolamento & purificação
Antozoários/microbiologia
Gammaproteobacteria/isolamento & purificação
Microbiota/genética
Spirochaetaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Alphaproteobacteria/genética
Animais
Sequência de Bases
DNA Bacteriano/genética
Feminino
Gammaproteobacteria/genética
Temperatura Alta
Masculino
Tipagem Molecular/métodos
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Spirochaetaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix013


  4 / 93 MEDLINE  
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[PMID]:27902274
[Au] Autor:Arroua B; Ranchou-Peyruse A; Ranchou-Peyruse M; Magot M; Urios L; Grimaud R
[Ad] Endereço:CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
[Ti] Título:Pleomorphochaeta caudata gen. nov., sp. nov., an anaerobic bacterium isolated from an offshore oil well, reclassification of Sphaerochaeta multiformis MO-SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov. as the type strain of this novel genus and emended description of the genus Sphaerochaeta.
[So] Source:Int J Syst Evol Microbiol;67(2):417-424, 2017 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strictly anaerobic Gram-stain-negative bacterium, designated strain SEBR 4223T, was isolated from the production water of an offshore Congolese oil field. Cells were non-motile, pleomorphic and had spherical, annular or budding shapes, often exhibiting long stalks. Strain SEBR 4223T grew on a range of carbohydrates, optimally at 37 °C and pH 7, in a medium containing 40 g l-1 NaCl. Predominant fatty acids were C14 : 0, C14 : 0 DMA, C16 : 0 and C16 : 1ω7c and the major polar lipids were phosphoglycolipids, phospholipids, glycolipids and diphosphatidylglycerol. The G+C content of the DNA was 28.7 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain SEBR 4223T and Sphaerochaeta multiformis MO-SPC2T formed a cluster with similarity to other species of the genus Sphaerochaeta of of less than 86 %. On the basis of the phenotypic characteristics and taxonomic analyses, we propose a novel genus, Pleomorphochaeta gen. nov., to accommodate the novel species Pleomorphochaeta caudata sp. nov., with SEBR 4223T (=DSM 103077T=JCM 31 475T) as the type strain. We also propose the reclassification of Sphaerochaeta multiformis MO SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov., the type strain of this novel genus and emend description of the genus Sphaerochaeta.
[Mh] Termos MeSH primário: Campos de Petróleo e Gás/microbiologia
Filogenia
Água do Mar/microbiologia
Spirochaetaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Congo
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Spirochaetaceae/genética
Spirochaetaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001641


  5 / 93 MEDLINE  
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[PMID]:26428233
[Au] Autor:Baek G; Kim J; Shin SG; Lee C
[Ad] Endereço:School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, 689-798, Republic of Korea.
[Ti] Título:Bioaugmentation of anaerobic sludge digestion with iron-reducing bacteria: process and microbial responses to variations in hydraulic retention time.
[So] Source:Appl Microbiol Biotechnol;100(2):927-37, 2016 Jan.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Although anaerobic digestion (AD) is a widely used option to manage waste activated sludge (WAS), there are some drawbacks related to its slow reaction rate and low energy productivity. This study examined an anaerobic WAS digester, augmented with an iron-reducing microbial consortium, relative to changes in microbial community structure and process performance at decreasing hydraulic retention times (HRTs) of 20 to 10 days. The enhanced methanation performance (approximately 40 % increase in methane yield) by the bioaugmentation was sustained until the HRT was decreased to 12.5 days, under Fe(3+)-rich conditions (ferric oxyhydroxide, 20 mM Fe). Enhanced iron-reducing activity was evidenced by the increased Fe(2+) to total Fe ratio maintained above 50 % during the stable operational phases. A further decrease in HRT to 10 days resulted in a significant performance deterioration, along with a drop in the Fe(2+) to total Fe ratio to <35 %, after four turnovers of operation. Prevailing existence of putative iron-reducing bacteria (IRBs) was identified by denaturing gradient gel electrophoresis (DGGE), with Spirochaetaceae- and Thauera-related organisms being dominant members, and clear dominance shifts among them with respect to decrease in HRT were observed. Lowering HRT led to evident shifts in bacterial community structure likely associated with washout of IRBs, leading to decreases in iron respiration activity and AD performance at a lower HRT. The bacterial community structure shifted dynamically over phases, and the community transitions correlated well with the changes in process performance. Overall, the combined biostimulation and bioaugmentation investigated in this study proved effective for enhanced methane recovery from anaerobic WAS digestion, which suggests an interesting potential for high-rate AD.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Reatores Biológicos
Ferro/metabolismo
Consórcios Microbianos/fisiologia
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Anaerobiose
Análise da Demanda Biológica de Oxigênio
Eletroforese em Gel de Gradiente Desnaturante
Spirochaetaceae/metabolismo
Eliminação de Resíduos Líquidos
Águas Residuais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sewage); 0 (Waste Water); E1UOL152H7 (Iron)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151003
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-015-7018-y


  6 / 93 MEDLINE  
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[PMID]:26341497
[Au] Autor:Troshina O; Oshurkova V; Suzina N; Machulin A; Ariskina E; Vinokurova N; Kopitsyn D; Novikov A; Shcherbakova V
[Ad] Endereço:1​ Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow region 142290, Russia.
[Ti] Título:Sphaerochaeta associata sp. nov., a spherical spirochaete isolated from cultures of Methanosarcina mazei JL01.
[So] Source:Int J Syst Evol Microbiol;65(12):4315-22, 2015 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An anaerobic, saccharolytic bacterial strain designated GLS2T was isolated from aggregates of the psychrotolerant archaeon Methanosarcina mazei strain JL01 isolated from arctic permafrost. Bacterial cells were non-motile, spherical, ovoid and annular with diameter 0.2-4 µm. They were chemoorganoheterotrophs using a wide range of mono-, di- and trisaccharides as carbon and energy sources. The novel isolate required yeast extract and vitamins for growth. The bacteria exhibited resistance to a number of ß-lactam antibiotics, rifampicin, streptomycin and vancomycin. Optimum growth was observed between 30 and 34 °C, at pH 6.8-7.5 and with 1-2 g NaCl l- 1. Isolate GLS2T was a strict anaerobe but it tolerated oxygen exposure. On the basis of 16S rRNA gene sequence similarity, strain GLS2T was shown to belong to the genus Sphaerochaeta within the family Spirochaetaceae. Its closest relatives were Sphaerochaeta globosa BuddyT (99.3 % 16S rRNA gene sequence similarity) and Sphaerochaeta pleomorpha GrapesT (95.4 % similarity). The G+C content of DNA was 47.2 mol%. The level of DNA-DNA hybridization between strains GLS2T and BuddyT was 34.7 ± 8.8 %. Major polar lipids were phosphoglycolipids, phospholipids and glycolipids; major fatty acids were C14 : 0, C16 : 0, C16 : 0 3-OH, C16 : 0 dimethyl acetal (DMA), C16 : 1n8 and C16 : 1 DMA; respiratory quinones were not detected. The results of DNA-DNA hybridization, physiological and biochemical tests demonstrated genotypic and phenotypic differentiation of strain GLS2T from the four species of the genus Sphaerochaeta with validly published names that allowed its separation into a new lineage at the species level. Strain GLS2T therefore represents a novel species, for which the name Sphaerochaeta associata sp. nov. is proposed, with the type strain GLS2T ( = DSM 26261T = VKM B-2742T).
[Mh] Termos MeSH primário: Methanosarcina
Pergelissolo/microbiologia
Filogenia
Spirochaetaceae/classificação
[Mh] Termos MeSH secundário: Regiões Árticas
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Spirochaetaceae/genética
Spirochaetaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160120
[Lr] Data última revisão:
160120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150906
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000575


  7 / 93 MEDLINE  
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[PMID]:25249566
[Au] Autor:Miyazaki M; Sakai S; Ritalahti KM; Saito Y; Yamanaka Y; Saito Y; Tame A; Uematsu K; Löffler FE; Takai K; Imachi H
[Ad] Endereço:Department of Subsurface Geobiology Analysis and Research (D-SUGAR), Japan Agency for Marine-Earth Science & Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan.
[Ti] Título:Sphaerochaeta multiformis sp. nov., an anaerobic, psychrophilic bacterium isolated from subseafloor sediment, and emended description of the genus Sphaerochaeta.
[So] Source:Int J Syst Evol Microbiol;64(Pt 12):4147-54, 2014 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An anaerobic, psychrophilic bacterium, strain MO-SPC2(T), was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediments collected from off the Shimokita Peninsula of Japan in the north-western Pacific Ocean. Cells were pleomorphic: spherical, annular, curved rod, helical and coccoid cell morphologies were observed. Motility only occurred in helical cells. Strain MO-SPC2(T) grew at 0-17 °C (optimally at 9 °C), at pH 6.0-8.0 (optimally at pH 6.8-7.2) and in 20-40 g NaCl l(-1) (optimally at 20-30 NaCl l(-1)). The strain grew chemo-organotrophically with mono-, di- and polysaccharides. The major end products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. The abundant polar lipids of strain MO-SPC2(T) were phosphatidylglycolipids, phospholipids and glycolipids. The major cellular fatty acids were C14 : 0, C16 : 0 and C16 : 1ω9. Isoprenoid quinones were not detected. The G+C content of the DNA was 32.3 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-SPC2(T) was affiliated with the genus Sphaerochaeta within the phylum Spirochaetes, and its closest relatives were Sphaerochaeta pleomorpha Grapes(T) (88.4 % sequence identity), Sphaerochaeta globosa Buddy(T) (86.7 %) and Sphaerochaeta coccoides SPN1(T) (85.4 %). Based on phenotypic characteristics and phylogenetic traits, strain MO-SPC2(T) is considered to represent a novel species of the genus Sphaerochaeta, for which the name Sphaerochaeta multiformis sp. nov. is proposed. The type strain is MO-SPC2(T) ( = JCM 17281(T) = DSM 23952(T)). An emended description of the genus Sphaerochaeta is also proposed.
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Filogenia
Spirochaetaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Japão
Dados de Sequência Molecular
Oceano Pacífico
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
Análise de Sequência de DNA
Spirochaetaceae/genética
Spirochaetaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:141210
[Lr] Data última revisão:
141210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140925
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.068148-0


  8 / 93 MEDLINE  
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[PMID]:24239193
[Au] Autor:Sikutová S; Bunková L; Krejcí E; Halouzka J; Sanogo YO; Rudolf I
[Ad] Endereço:Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Kvetná 8, 603 65 Brno, Czech Republic.
[Ti] Título:Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito.
[So] Source:Microbiol Res;169(5-6):348-52, 2014 May-Jun.
[Is] ISSN:1618-0623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33 °C. Strain BR91 was sensitive to trimethoprim, nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and α-fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed several major proteins in the size range of 13-16 kDa, 22-30 kDa and 37-131 kDa. Fatty acid methyl ester (FAME) analysis showed that C14:0, C16:0, C18:1 ω9c and summed feature 5 (C18:2 ω6,9c and/or C18:0 anteiso) are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and the Lyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents a unique taxonomical entity in a system of spirochaetal species.
[Mh] Termos MeSH primário: Culex/microbiologia
Spirochaetaceae/classificação
Spirochaetaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Proteínas de Bactérias/análise
Borrelia burgdorferi
Culicidae
Meios de Cultura/química
Enzimas/análise
Ácidos Graxos/análise
Testes de Sensibilidade Microbiana
Spirochaetaceae/química
Spirochaetaceae/fisiologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Culture Media); 0 (Enzymes); 0 (Fatty Acids)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140325
[Lr] Data última revisão:
140325
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131119
[St] Status:MEDLINE


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Fotocópia
[PMID]:23156694
[Au] Autor:Tulupova Iu; Parfenova VV; Sitnikova TIa; Sorokovikova EG; Khanaev IB
[Ti] Título:[First report on bacteria of the family Spirochaetaceae from digestive tract of endemic gastropods from Lake Baikal].
[So] Source:Mikrobiologiia;81(4):500-7, 2012 Jul-Aug.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Mh] Termos MeSH primário: Gastrópodes/microbiologia
Spirochaetaceae
[Mh] Termos MeSH secundário: Animais
Trato Gastrointestinal/microbiologia
Lagos/microbiologia
Microscopia Eletrônica de Transmissão
Federação Russa
Sibéria
Spirochaetaceae/classificação
Spirochaetaceae/isolamento & purificação
Spirochaetaceae/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1212
[Cu] Atualização por classe:121119
[Lr] Data última revisão:
121119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121120
[St] Status:MEDLINE


  10 / 93 MEDLINE  
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PubMed Central Texto completo
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[PMID]:21040513
[Au] Autor:Xie G; Chain PS; Lo CC; Liu KL; Gans J; Merritt J; Qi F
[Ad] Endereço:Oralgen Database, Genome Science Group, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. xie@lanl.gov
[Ti] Título:Community and gene composition of a human dental plaque microbiota obtained by metagenomic sequencing.
[So] Source:Mol Oral Microbiol;25(6):391-405, 2010 Dec.
[Is] ISSN:2041-1014
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Human dental plaque is a complex microbial community containing an estimated 700 to 19,000 species/phylotypes. Despite numerous studies analysing species richness in healthy and diseased human subjects, the true genomic composition of the human dental plaque microbiota remains unknown. Here we report a metagenomic analysis of a healthy human plaque sample using a combination of second-generation sequencing platforms. A total of 860 million base pairs of non-human sequences were generated. Various analysis tools revealed the presence of 12 well-characterized phyla, members of the TM-7 and BRC1 clade, and sequences that could not be classified. Both pathogens and opportunistic pathogens were identified, supporting the ecological plaque hypothesis for oral diseases. Mapping the metagenomic reads to sequenced reference genomes demonstrated that 4% of the reads could be assigned to the sequenced species. Preliminary annotation identified genes belonging to all known functional categories. Interestingly, although 73% of the total assembled contig sequences were predicted to code for proteins, only 51% of them could be assigned a functional role. Furthermore, ~2.8% of the total predicted genes coded for proteins involved in resistance to antibiotics and toxic compounds, suggesting that the oral cavity is an important reservoir for antimicrobial resistance.
[Mh] Termos MeSH primário: Placa Dentária/microbiologia
Genoma Bacteriano/genética
Metagenoma/genética
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Bactérias/classificação
Proteínas de Bactérias/genética
Bacteroidetes/classificação
Mapeamento Cromossômico
Mapeamento de Sequências Contíguas/métodos
Cianobactérias/classificação
Bases de Dados de Ácidos Nucleicos
Farmacorresistência Bacteriana/genética
Ecossistema
Fibrobacter/classificação
Fusobactérias/classificação
Trato Gastrointestinal/microbiologia
Seres Humanos
Metagenômica/métodos
Proteobactérias/classificação
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Spirochaetaceae/classificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1103
[Cu] Atualização por classe:161203
[Lr] Data última revisão:
161203
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:101103
[St] Status:MEDLINE
[do] DOI:10.1111/j.2041-1014.2010.00587.x



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