Base de dados : MEDLINE
Pesquisa : B03.440.425.410.711.193 [Categoria DeCS]
Referências encontradas : 1863 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 187 ir para página                         

  1 / 1863 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29178531
[Au] Autor:Kwan JY; Griggs R; Chicana B; Miller C; Swei A
[Ad] Endereço:Department of Biology, San Francisco State University, San Francisco, CA, USA.
[Ti] Título:Vertical vs. horizontal transmission of the microbiome in a key disease vector, Ixodes pacificus.
[So] Source:Mol Ecol;26(23):6578-6589, 2017 Dec.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Vector-borne pathogens are increasingly found to interact with the vector's microbiome, influencing disease transmission dynamics. However, the processes that regulate the formation and development of the microbiome are largely unexplored for most tick species, an emerging group of disease vectors. It is not known how much of the tick microbiome is acquired through vertical transmission vs. horizontally from the environment or interactions with bloodmeal sources. Using 16S rRNA sequencing, we examined the microbiome of Ixodes pacificus, the vector of Lyme disease in the western USA, across life stages and infection status. We also characterized microbiome diversity in field and laboratory-collected nymphal ticks to determine how the surrounding environment affects microbiome diversity. We found a decrease in both species richness and evenness as the tick matures from larva to adult. When the dominant Rickettsial endosymbiont was computationally removed from the tick microbial community, we found that infected nymphs had lower species evenness than uninfected ticks, suggesting that lower microbiome diversity is associated with pathogen transmission in wild-type ticks. Furthermore, laboratory-reared nymph microbiome diversity was found to be compositionally distinct and significantly depauperate relative to field-collected nymphs. These results highlight unique patterns in the microbial community of I. pacificus that is distinct from other tick species. We provide strong evidence that ticks acquire a significant portion of their microbiome through exposure to their environment despite a loss of overall diversity through life stages. We provide evidence that loss of microbial diversity is at least in part due to elimination of microbial diversity with bloodmeal feeding but other factors may also play a role.
[Mh] Termos MeSH primário: Ixodes/microbiologia
Microbiota
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Borrelia/classificação
California
Vetores de Doenças
Feminino
Larva/microbiologia
Masculino
Ninfa/microbiologia
RNA Ribossômico 16S/genética
Rickettsia/classificação
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1111/mec.14391


  2 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29190046
[Au] Autor:Hytönen J; Khawaja T; Grönroos JO; Jalava A; Meri S; Oksi J
[Ti] Título:Relapsing fever.
[So] Source:Duodecim;132(21):1952-6, 2016.
[Is] ISSN:0012-7183
[Cp] País de publicação:Finland
[La] Idioma:eng
[Ab] Resumo:Relapsing fewer is an infection to be considered in the differential diagnosis of an immigrant´s febrile illness. It is a severe, tick-borne or body louse-borne infection caused by the relapsing fever associated borrelia species. The body louse-borne infection is in particular encountered in the Horn of Africa region due to poor hygiene, and has during the past year been described in several European countries as imported by refugees coming from this region. Doctors should thus bear relapsing fever in mind as a differential diagnosis in a febrile refugee having recently arrived in Finland.
[Mh] Termos MeSH primário: Borrelia
Infestações por Piolhos/diagnóstico
Refugiados
Febre Recorrente/diagnóstico
Infestações por Carrapato/diagnóstico
[Mh] Termos MeSH secundário: Animais
Diagnóstico Diferencial
Seres Humanos
Viagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  3 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28455331
[Au] Autor:Schötta AM; Wijnveld M; Stockinger H; Stanek G
[Ad] Endereço:Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria anna-margarita.schoetta@meduniwien.ac.at.
[Ti] Título:Approaches for Reverse Line Blot-Based Detection of Microbial Pathogens in Ixodes ricinus Ticks Collected in Austria and Impact of the Chosen Method.
[So] Source:Appl Environ Microbiol;83(13), 2017 Jul 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ticks transmit a large number of pathogens capable of causing human disease. In this study, the PCR-reverse line blot (RLB) method was used to screen for pathogens in a total of 554 ticks collected from all provinces of Austria. These pathogens belong to the genera , , / (including " Neoehrlichia"), , and The pathogens with the highest detected prevalence were spirochetes of the complex, in 142 ticks (25.6%). (80/142) was the most frequently detected species, followed by (38/142) and (36/142). , , and were found in 28 ticks, 5 ticks, and 1 tick, respectively. spp. were detected in 93 ticks (16.8%): (39/93), (38/93), (2/93), and (1/93). Thirteen samples remain uncharacterized. " Neoehrlichia mikurensis," spp. ( , , ), and were found in 4.5%, 2.7%, and 0.7%, respectively. was not detected. Multiple microorganisms were detected in 40 ticks (7.2%), and the cooccurrence of spp. and " Neoehrlichia mikurensis" showed a significant positive correlation. We also compared different PCR-RLBs for detection of and spp. and showed that different detection approaches provide highly diverse results, indicating that analysis of environmental samples remains challenging. This study determined the wide spectrum of tick-borne bacterial and protozoal pathogens that can be encountered in Austria. Surveillance of (putative) pathogenic microorganisms occurring in the environment is of medical importance, especially when those agents can be transmitted by ticks and cause disease. The observation of significant coinfections of certain microorganisms in field-collected ticks is an initial step to an improved understanding of microbial interactions in ticks. In addition, we show that variations in molecular detection methods, such as in primer pairs and target genes, can considerably influence the final results. For instance, detection of certain genospecies of borreliae may be better or worse by one method or the other, a fact of great importance for future screening studies.
[Mh] Termos MeSH primário: Anaplasma/isolamento & purificação
Southern Blotting/métodos
Borrelia/isolamento & purificação
Ixodes/microbiologia
Reação em Cadeia da Polimerase/métodos
Rickettsia/isolamento & purificação
[Mh] Termos MeSH secundário: Anaplasma/classificação
Anaplasma/genética
Animais
Áustria
Borrelia/classificação
Borrelia/genética
DNA Bacteriano/genética
Rickettsia/classificação
Rickettsia/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  4 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28902847
[Au] Autor:Marosevic D; Margos G; Wallich R; Wieser A; Sing A; Fingerle V
[Ad] Endereço:German National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority (LGL), Oberschleißheim, Germany.
[Ti] Título:First insights in the variability of Borrelia recurrentis genomes.
[So] Source:PLoS Negl Trop Dis;11(9):e0005865, 2017 Sep.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Borrelia recurrentis is the causative agent of louse-borne relapsing fever, endemic to the Horn of Africa. New attention was raised in Europe, with the highest number of cases (n = 45) reported among migrants in 2015 in Germany and sporadically from other European countries. So far only one genome was sequenced, hindering the development of specific molecular diagnostic and typing tools. Here we report on modified culture conditions for B. recurrentis and the intraspecies genome variability of six isolates isolated and cultured in different years in order to explore the possibility to identify new targets for typing and examine the molecular epidemiology of the pathogen. METHODOLOGY/PRINCIPAL FINDINGS: Two historical isolates from Ethiopia and four isolates from migrants from Somalia (n = 3) and Ethiopia (n = 1) obtained in 2015 were cultured in MPK-medium supplemented with 50% foetal calf serum. Whole DNA was sequenced using Illumina MiSeq technology and analysed using the CLC Genomics Workbench and SPAdes de novo assembler. Compared to the reference B. recurrentis A1 29-38 SNPs were identified in the genome distributed on the chromosome and plasmids. In addition to that, plasmids of differing length, compared to the available reference genome were identified. CONCLUSIONS/SIGNIFICANCE: The observed low genetic variability of B. recurrentis isolates is possibly due to the adaptation to a very conserved vector-host (louse-human) cycle, or influenced by the fastidious nature of the pathogen and their resistance to in vitro growth. Nevertheless, isolates obtained in 2015 were bearing the same chromosomal SNPs and could be distinguished from the historical isolates by means of whole genome sequencing, but not hitherto used typing methods. This is the first study examining the molecular epidemiology of B. recurrentis and provides the necessary background for the development of better diagnostic tools.
[Mh] Termos MeSH primário: Borrelia/genética
Genoma Bacteriano
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Cromossomos Bacterianos
Seres Humanos
Filogenia
Plasmídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005865


  5 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28898274
[Au] Autor:Kumar B; Miller K; Charon NW; Legleiter J
[Ad] Endereço:The C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, West Virginia, United States of America.
[Ti] Título:Periplasmic flagella in Borrelia burgdoferi function to maintain cellular integrity upon external stress.
[So] Source:PLoS One;12(9):e0184648, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tapping mode atomic force microscopy (AFM) in solution was used to analyze the role of the internally located periplasmic flagella (PFs) of the Lyme disease spirochete Borrelia burgdorferi in withstanding externally applied cellular stresses. By systematically imaging immobilized spirochetes with increasing tapping forces, we found that mutants that lack PFs are more readily compressed and damaged by the imaging process compared to wild-type cells. This finding suggest that the PFs, aside from being essential for motility and involved in cell shape, play a cytoskeletal role in dissipating energy and maintaining cellular integrity in the presence of external stress.
[Mh] Termos MeSH primário: Borrelia/metabolismo
Flagelos/metabolismo
Estresse Fisiológico
[Mh] Termos MeSH secundário: Borrelia/genética
Borrelia/ultraestrutura
Flagelos/genética
Flagelos/ultraestrutura
Movimento
Mutação
Estresse Mecânico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184648


  6 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28874019
[Au] Autor:Khoo JJ; Lim FS; Tan KK; Chen FS; Phoon WH; Khor CS; Pike BL; Chang LY; AbuBakar S
[Ad] Endereço:Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, 50603, Malaysia.
[Ti] Título:Detection in Malaysia of a Borrelia sp. From Haemaphysalis hystricis (Ixodida: Ixodidae).
[So] Source:J Med Entomol;54(5):1444-1448, 2017 Sep 01.
[Is] ISSN:1938-2928
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spirochetes from the Borrelia genus are known to cause diseases in humans, namely Lyme disease and relapsing fever. These organisms are commonly transmitted to humans by arthropod vectors including ticks, mite, and lice. Here, we report the molecular detection of a Borrelia sp. from a Haemaphysalis hystricis Supino tick collected from wildlife in an Orang Asli settlement in Selangor, Malaysia. Phylogenetic analyses of partial 16s rRNA and flaB gene sequences revealed that the Borrelia sp. is closely related to the relapsing fever group borreliae, Borrelia lonestari, Borrelia miyamotoi, and Borrelia theileri, as well as a number of uncharacterized Borrelia sp. from ticks in Portugal and Japan. To our knowledge, this is the first report of a Borrelia sp. detected in H. hystricis, and in Malaysia. The zoonotic potential of this Borrelia sp. merits further investigation.
[Mh] Termos MeSH primário: Borrelia/classificação
Borrelia/isolamento & purificação
Ixodidae/microbiologia
[Mh] Termos MeSH secundário: Animais
Borrelia/genética
Flagelina/genética
Malásia
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Análise de Sequência de RNA
Sus scrofa/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 12777-81-0 (Flagellin); 140470-87-7 (flaB flagellin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjx131


  7 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28874016
[Au] Autor:Dolan MC; Breuner NE; Hojgaard A; Boegler KA; Hoxmeier JC; Replogle AJ; Eisen L
[Ad] Endereço:Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521.
[Ti] Título:Transmission of the Lyme Disease Spirochete Borrelia mayonii in Relation to Duration of Attachment by Nymphal Ixodes scapularis (Acari: Ixodidae).
[So] Source:J Med Entomol;54(5):1360-1364, 2017 Sep 01.
[Is] ISSN:1938-2928
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The recently recognized Lyme disease spirochete, Borrelia mayonii, has been detected in host-seeking Ixodes scapularis Say ticks and is associated with human disease in the Upper Midwest. Although experimentally shown to be vector competent, studies have been lacking to determine the duration of time from attachment of a single B. mayonii-infected I. scapularis nymph to transmission of spirochetes to a host. If B. mayonii spirochetes were found to be transmitted within the first 24 h after tick attachment, in contrast to Borrelia burgdorferi spirochetes (>24 h), then current recommendations for tick checks and prompt tick removal as a way to prevent transmission of Lyme disease spirochetes would need to be amended. We therefore conducted a study to determine the probability of transmission of B. mayonii spirochetes from single infected nymphal I. scapularis ticks to susceptible experimental mouse hosts at three time points postattachment (24, 48, and 72 h) and for a complete feed (>72-96 h). No evidence of infection with or exposure to B. mayonii occurred in mice that were fed upon by a single infected nymph for 24 or 48 h. The probability of transmission by a single infected nymphal tick was 31% after 72 h of attachment and 57% for a complete feed. In addition, due to unintended simultaneous feeding upon some mice by two B. mayonii-infected nymphs, we recorded a single occasion in which feeding for 48 h by two infected nymphs resulted in transmission and viable infection in the mouse. We conclude that the duration of attachment of a single infected nymphal I. scapularis tick required for transmission of B. mayonii appears to be similar to that for B. burgdorferi: transmission is minimal for the first 24 h of attachment, rare up to 48 h, but then increases distinctly by 72 h postattachment.
[Mh] Termos MeSH primário: Vetores Aracnídeos/microbiologia
Borrelia/fisiologia
Ixodes/microbiologia
Doença de Lyme/transmissão
[Mh] Termos MeSH secundário: Animais
Vetores Aracnídeos/crescimento & desenvolvimento
Feminino
Ixodes/crescimento & desenvolvimento
Doença de Lyme/microbiologia
Camundongos
Ninfa/crescimento & desenvolvimento
Ninfa/microbiologia
Probabilidade
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjx089


  8 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28719293
[Au] Autor:Yamano K; Ito T; Kiyanagi K; Yamazaki H; Sugawara M; Saito T; Ohashi N; Zamoto-Niikura A; Sato K; Kawabata H
[Ad] Endereço:Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo Japan.
[Ti] Título:Case Report: Clinical Features of a Case of Suspected Disease in Hokkaido, Japan.
[So] Source:Am J Trop Med Hyg;97(1):84-87, 2017 Jul.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We herein report a case of suspected disease in Hokkaido, Japan. The patient complained of lassitude, arthralgia, and high fever after a tick bite. Furthermore, at the time of consultation, the patient exhibited momentary loss of consciousness and low blood pressure. Laboratory tests revealed elevation of liver enzymes, thrombocytopenia, and increased C-reactive protein. Seroconversion to glycerophosphoryl diester phosphodiesterase antigen suggested the patient was infected with a relapsing fever group species.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Borrelia/isolamento & purificação
Febre Recorrente/diagnóstico
Febre Recorrente/tratamento farmacológico
Doenças Transmitidas por Carrapatos/diagnóstico
Doenças Transmitidas por Carrapatos/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Animais
Seres Humanos
Japão
Masculino
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0699


  9 / 1863 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28656008
[Au] Autor:Crowder CD; Denny RL; Barbour AG
[Ad] Endereço:Departments of Medicine and Microbiology & Molecular Genetics University of California Irvine, Irvine, California.
[Ti] Título:Segregation Lag in Polyploid Cells of the Pathogen Genus : Implications for Antigenic Variation
.
[So] Source:Yale J Biol Med;90(2):195-218, 2017 Jun.
[Is] ISSN:1551-4056
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Relapsing fever agents like undergo multiphasic antigenic variation that is attributable to spontaneous DNA non-reciprocal transpositions at a particular locus in the genome. This genetic switch results in a new protein being expressed on the cell surface, allowing cells with that phenotype to escape prevailing immunity. But the switch occurs in only one of several genomes in these spirochetes, and a newly-switched gene is effectively "recessive" until homozygosity is achieved. The longer that descendants of the switched cell expressed both old and new proteins, the longer this lineage risks neutralization by antibody to the old protein. We investigated the implications for antigenic variation of the phenotypic lag that polyploidy would confer on cells. We first experimentally determined the average genome copy number in daughter cells after division during mouse infection with strain HS1. We then applied discrete deterministic and stochastic simulations to predict outcomes when genomes were equably segregated either linearly, i.e. according to their position in one-dimensional arrays, or randomly partitioned, as for a sphere. Linear segregation replication provided for a lag in achievement of homozygosity that was significantly shorter than could be achieved under the random segregation condition. For cells with 16 genomes, this would be a 4-generation lag. A model incorporating the immune response and evolved matrices of switch rates indicated a greater fitness for polyploid over monoploid bacteria in terms of duration of infection.
[Mh] Termos MeSH primário: Variação Antigênica/fisiologia
Borrelia/fisiologia
[Mh] Termos MeSH secundário: Animais
Variação Antigênica/genética
Antígenos de Bactérias/genética
Antígenos de Bactérias/imunologia
Borrelia/citologia
Borrelia/genética
Borrelia/imunologia
Feminino
Genoma Bacteriano/genética
Camundongos
Camundongos SCID/microbiologia
Microscopia de Contraste de Fase
Reação em Cadeia da Polimerase
Poliploidia
Febre Recorrente/imunologia
Febre Recorrente/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


  10 / 1863 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28651265
[Au] Autor:Örgel A; Hauser TK; Nägele T; Horger M
[Ti] Título:[Imaging in Cranial Nerve Neuritis by Lyme-Neuroborreliosis].
[Ti] Título:Neuritis bei Lyme-Neuroborreliose..
[So] Source:Rofo;189(7):599-602, 2017 07.
[Is] ISSN:1438-9010
[Cp] País de publicação:Germany
[La] Idioma:ger
[Mh] Termos MeSH primário: Borrelia
Doenças dos Nervos Cranianos/diagnóstico por imagem
Neuroborreliose de Lyme/diagnóstico por imagem
Imagem por Ressonância Magnética/métodos
Neurite (Inflamação)/diagnóstico por imagem
[Mh] Termos MeSH secundário: Diagnóstico Diferencial
Medicina Baseada em Evidências
Seres Humanos
Aumento da Imagem/métodos
Tomografia Computadorizada por Raios X/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1055/s-0043-104535



página 1 de 187 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde