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[PMID]: 28800622 [Au] Autor: Belstrøm D; Sembler-Møller ML; Grande MA; Kirkby N; Cotton SL; Paster BJ; Holmstrup P [Ad] Endereço: Section for Periodontology, Microbiology, and Community Dentistry, Department of Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. [Ti] Título: Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients. [So] Source: PLoS One;12(8):e0182992, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: OBJECTIVES: The purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. We tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens. DESIGN: Site specific subgingival plaque samples (n = 54), pooled subgingival plaque samples (n = 18) and stimulated saliva samples (n = 18) were collected from 18 patients with generalized chronic periodontitis. Subgingival and salivary microbiotas were characterized by means of HOMINGS (Human Oral Microbe Identification using Next Generation Sequencing) and microbial community profiles were compared using Spearman rank correlation coefficient. RESULTS: Pronounced intraindividual differences were recorded in site-specific microbial profiles, and site-specific information was in general not reflected by pooled subgingival samples. Presence of Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia, Filifactor alocis, Tannerella forsythia and Parvimona micra in site-specific subgingival samples were detected in saliva with an AUC of 0.79 (sensitivity: 0.61, specificity: 0.94), compared to an AUC of 0.76 (sensitivity: 0.56, specificity: 0.94) in pooled subgingival samples. CONCLUSIONS: Site-specific presence of periodontal pathogens was detected with comparable accuracy in stimulated saliva samples and pooled subgingival plaque samples. Consequently, saliva may be a reasonable surrogate for pooled subgingival samples when screening for presence of periopathogens. Future large-scale studies are needed to confirm findings from this study. [Mh] Termos MeSH primário: DNA Bacteriano/genética Filogenia Porphyromonas gingivalis/genética Prevotella intermedia/genética Tannerella forsythia/genética Treponema denticola/genética [Mh] Termos MeSH secundário: Adulto Idoso Periodontite Crônica/diagnóstico Periodontite Crônica/microbiologia Placa Dentária/diagnóstico Placa Dentária/microbiologia Feminino Sequenciamento de Nucleotídeos em Larga Escala Seres Humanos Masculino Meia-Idade Bolsa Periodontal/diagnóstico Bolsa Periodontal/microbiologia Porphyromonas gingivalis/classificação Porphyromonas gingivalis/isolamento & purificação Prevotella intermedia/classificação Prevotella intermedia/isolamento & purificação Saliva/química Saliva/microbiologia Tannerella forsythia/classificação Tannerella forsythia/isolamento & purificação Treponema denticola/classificação Treponema denticola/isolamento & purificação [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171006 [Lr] Data última revisão: 171006 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170812 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0182992

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[PMID]: 28089378 [Au] Autor: Kataoka N; Vangnai AS; Pongtharangkul T; Yakushi T; Matsushita K [Ad] Endereço: Division of Agricultural Sciences, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan; Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: nkataoka@yamaguchi-u.ac.jp. [Ti] Título: Butyrate production under aerobic growth conditions by engineered Escherichia coli. [So] Source: J Biosci Bioeng;123(5):562-568, 2017 May. [Is] ISSN: 1347-4421 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Butyrate is an important industrial platform chemical. Although several groups have reported butyrate production under oxygen-limited conditions by a native producer, Clostridium tyrobutylicum, and by a metabolically engineered Escherichia coli, efforts to produce butyrate under aerobic growth conditions have met limited success. Here, we constructed a novel butyrate synthetic pathway that functions under aerobic growth conditions in E. coli, by modifying the 1-butanol synthetic pathway reported previously. The pathway consists of phaA (acetyltransferase) and phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, phaJ ((R)-specific enoyl-CoA hydratase) from Aeromonas caviae, ter (trans-enoyl-CoA reductase) from Treponema denticola, and endogenous thioesterase(s) of E. coli. To evaluate the potential of this pathway for butyrate production, culture conditions, including pH, oxygen supply, and concentration of inorganic nitrogen sources, were optimized in a mini-jar fermentor. Under the optimal conditions, butyrate was produced at a concentration of up to 140 mM (12.3 g/L in terms of butyric acid) after 54 h of fed-batch culture. [Mh] Termos MeSH primário: Reatores Biológicos Vias Biossintéticas/genética Ácido Butírico/metabolismo Escherichia coli/metabolismo Engenharia Metabólica [Mh] Termos MeSH secundário: 1-Butanol/metabolismo Acetiltransferases/genética Acetiltransferases/metabolismo Aerobiose Aeromonas caviae/enzimologia Aeromonas caviae/genética Oxirredutases do Álcool/genética Oxirredutases do Álcool/metabolismo Técnicas de Cultura Celular por Lotes Clostridium/metabolismo Cupriavidus necator/enzimologia Cupriavidus necator/genética Enoil-CoA Hidratase/genética Enoil-CoA Hidratase/metabolismo Escherichia coli/enzimologia Escherichia coli/genética Escherichia coli/crescimento & desenvolvimento Concentração de Íons de Hidrogênio Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo Oxigênio/metabolismo Oxigênio/farmacologia Tioléster Hidrolases/genética Tioléster Hidrolases/metabolismo Treponema denticola/enzimologia Treponema denticola/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 107-92-6 (Butyric Acid); 8PJ61P6TS3 (1-Butanol); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.36 (acetoacetyl-CoA reductase); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.1.38 (trans-2-enoyl-CoA reductase (NADPH)); EC 2.3.1.- (Acetyltransferases); EC 3.1.2.- (Thiolester Hydrolases); EC 4.2.1.- (R-specific trans-2,3-enoylacyl-CoA hydratase); EC 4.2.1.17 (Enoyl-CoA Hydratase); S88TT14065 (Oxygen) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170509 [Lr] Data última revisão: 170509 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170117 [St] Status: MEDLINE

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[PMID]: 28068479 [Au] Autor: Choi JW; Kim SC; Hong SH; Lee HJ [Ad] Endereço: 1 Department of Oral Microbiology and Immunology, School of Dentistry, Kyungpook National University, Daegu, South Korea. [Ti] Título: Secretable Small RNAs via Outer Membrane Vesicles in Periodontal Pathogens. [So] Source: J Dent Res;96(4):458-466, 2017 Apr. [Is] ISSN: 1544-0591 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: MicroRNAs (miRNAs) have been shown to be major regulators of eukaryotic gene expression. However, bacterial RNAs comparable in size to eukaryotic miRNAs (18-22 nucleotides) have received little attention. Recently, a novel class of small RNAs similar in size to miRNAs (miRNA-size, small RNAs or msRNAs) have also been found in several bacteria. Like miRNAs, msRNAs are approximately 15 to 25 nucleotides in length, and their precursors are predicted to form a hairpin loop secondary structure. Here, we identified msRNAs in the periodontal pathogens Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola. We examined these msRNAs using a deep sequencing method and characterized dozens of msRNAs through bioinformatic analysis. Highly expressed msRNAs were selected for further validation. The findings suggest that this class of small RNAs is well conserved across the domains of life. Indeed, msRNAs secreted via bacterial outer membrane vesicles (OMVs) were detected. The ability of bacterial OMVs to deliver RNAs into eukaryotic cells was also observed. These msRNAs in OMVs allowed us to identify their potential human immune-related target genes. Furthermore, we found that exogenous msRNAs could suppress expression of certain cytokines in Jurkat T cells. We propose msRNAs may function as novel bacterial signaling molecules that mediate bacteria-to-human interactions. Furthermore, this study may provide fresh insight into bacterial pathogenic mechanisms of periodontal diseases. [Mh] Termos MeSH primário: Aggregatibacter actinomycetemcomitans/genética Vesículas Citoplasmáticas/metabolismo MicroRNAs/análise Doenças Periodontais/microbiologia Porphyromonas gingivalis/genética RNA Bacteriano/análise Treponema denticola/genética [Mh] Termos MeSH secundário: Citocinas/análise Regulação Bacteriana da Expressão Gênica Seres Humanos Microscopia Confocal Microscopia Eletrônica de Transmissão Reação em Cadeia da Polimerase [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cytokines); 0 (MicroRNAs); 0 (RNA, Bacterial) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170531 [Lr] Data última revisão: 170531 [Sb] Subgrupo de revista: D; IM [Da] Data de entrada para processamento: 170110 [St] Status: MEDLINE [do] DOI: 10.1177/0022034516685071

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[PMID]: 28009086 [Au] Autor: Vences-Guzmán MÁ; Paula Goetting-Minesky M; Guan Z; Castillo-Ramirez S; Córdoba-Castro LA; López-Lara IM; Geiger O; Sohlenkamp C; Christopher Fenno J [Ad] Endereço: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n, Apdo. Postal 565-A, Cuernavaca, Morelos, CP62210, Mexico. [Ti] Título: 1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway. [So] Source: Mol Microbiol;103(5):896-912, 2017 Mar. [Is] ISSN: 1365-2958 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Treponema denticola synthesizes phosphatidylcholine through a licCA-dependent CDP-choline pathway identified only in the genus Treponema. However, the mechanism of conversion of CDP-choline to phosphatidylcholine remained unclear. We report here characterization of TDE0021 (herein designated cpt) encoding a 1,2-diacylglycerol choline phosphotransferase homologous to choline phosphotransferases that catalyze the final step of the highly conserved Kennedy pathway for phosphatidylcholine synthesis in eukaryotes. T. denticola Cpt catalyzed in vitro phosphatidylcholine formation from CDP-choline and diacylglycerol, and full activity required divalent manganese. Allelic replacement mutagenesis of cpt in T. denticola resulted in abrogation of phosphatidylcholine synthesis. T. denticola Cpt complemented a Saccharomyces cerevisiae CPT1 mutant, and expression of the entire T. denticola LicCA-Cpt pathway in E. coli resulted in phosphatidylcholine biosynthesis. Our findings show that T. denticola possesses a unique phosphatidylcholine synthesis pathway combining conserved prokaryotic choline kinase and CTP:phosphocholine cytidylyltransferase activities with a 1,2-diacylglycerol choline phosphotransferase that is common in eukaryotes. Other than in a subset of mammalian host-associated Treponema that includes T. pallidum, this pathway is found in neither bacteria nor Archaea. Molecular dating analysis of the Cpt gene family suggests that a horizontal gene transfer event introduced this gene into an ancestral Treponema well after its divergence from other spirochetes. [Mh] Termos MeSH primário: Vias Biossintéticas Diacilglicerol Colinofosfotransferase/metabolismo Fosfatidilcolinas/biossíntese Treponema denticola/metabolismo [Mh] Termos MeSH secundário: Alelos Vias Biossintéticas/genética Vias Biossintéticas/fisiologia Catálise Cinética Manganês/metabolismo Mutagênese Alinhamento de Sequência Treponema denticola/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Phosphatidylcholines); 42Z2K6ZL8P (Manganese); EC 2.7.8.2 (Diacylglycerol Cholinephosphotransferase) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171114 [Lr] Data última revisão: 171114 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161224 [St] Status: MEDLINE [do] DOI: 10.1111/mmi.13596

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[PMID]: 27914958 [Au] Autor: Nagano K; Hasegawa Y; Yoshida Y; Yoshimura F [Ad] Endereço: Department of Microbiology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan. Electronic address: nagano@dpc.agu.ac.jp. [Ti] Título: Comparative analysis of motility and other properties of Treponema denticola strains. [So] Source: Microb Pathog;102:82-88, 2017 Jan. [Is] ISSN: 1096-1208 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The periodontitis-associated pathogen Treponema denticola is a spirochetal bacterium that swims by rotating its cell body like a corkscrew using periplasmic flagella. We compared physiologic and pathogenic properties, including motility, in four strains of T. denticola. Phase-contrast microscopy showed differential motility between the strains; ATCC 35404 showed the highest motility, followed by ATCC 33521, and the remaining two strains (ATCC 35405 and ATCC 33520) showed the lowest motility. Transmission electron microscopy showed that the low motility strains exhibited extracellular flagellar protrusions resulting from elongated flagella. Treponemal flagellar filaments are composed of three flagellins of FlaB1, FlaB2 and FlaB3. FlaB1 expression was comparable between the strains, whereas FlaB2 expression was lowest in ATCC 35404. FlaB3 expression varied among strains, with ATCC 35405, ATCC 33520, ATCC 33521, and ATCC 35404 showing the highest to lowest expression levels, respectively. Additionally, the low motility strains showed faster electrophoretic mobility of FlaB3, suggesting that posttranslational modifications of these proteins may have varied, because the amino acid sequences of FlaB3 were identical between the strains. These results suggest that inappropriate expression of FlaB2 and FlaB3 caused the unusual elongation of flagella that resulted in decreased motility. Furthermore, the low motility strains grew to higher bacterial density, and showed greater chymotrypsin-like protease activity, and more bacterial cells associated with gingival epithelial cells in comparison with the high motility strains. There may be a relationship between motility and these properties, but the genetic factors underlying this association remain unclear. [Mh] Termos MeSH primário: Fenômenos Fisiológicos Bacterianos Treponema denticola/fisiologia [Mh] Termos MeSH secundário: Proteínas de Bactérias/química Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Linhagem Celular Biologia Computacional/métodos Células Epiteliais/microbiologia Regulação Bacteriana da Expressão Gênica Infecções por Bactérias Gram-Negativas/microbiologia Seres Humanos Técnicas In Vitro Peptídeo Hidrolases/metabolismo Doenças Periodontais/microbiologia Fenótipo Transcrição Genética Treponema denticola/ultraestrutura [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); EC 3.4.- (Peptide Hydrolases) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170406 [Lr] Data última revisão: 170406 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161205 [St] Status: MEDLINE

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[PMID]: 27899684 [Au] Autor: Bale BF; Doneen AL; Vigerust DJ [Ad] Endereço: Texas Tech Health Science Center, School of Nursing, Lubbock, Texas, USA. [Ti] Título: High-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. [So] Source: Postgrad Med J;93(1098):215-220, 2017 Apr. [Is] ISSN: 1469-0756 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Periodontal disease (PD) is generated by microorganisms. These microbes can enter the general circulation causing a bacteraemia. The result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. Level A evidence supports that PD is independently associated with arterial disease. PD is a common chronic condition affecting the majority of Americans 30 years of age and older. Atherosclerosis remains the largest cause of death and disability. Studies indicate that the adverse cardiovascular effects from PD are due to a few putative or high-risk bacteria: , , , or There are three accepted essential elements in the pathogenesis of atherosclerosis: lipoprotein serum concentration, endothelial permeability and binding of lipoproteins in the arterial intima. There is scientific evidence that PD caused by the high-risk pathogens can influence the pathogenesis triad in an adverse manner. With this appreciation, it is reasonable to state PD, due to high-risk pathogens, is a contributory cause of atherosclerosis. Distinguishing this type of PD as causal provides a significant opportunity to reduce arterial disease. [Mh] Termos MeSH primário: Aggregatibacter actinomycetemcomitans/patogenicidade Periodontite Agressiva/complicações Doença da Artéria Coronariana/etiologia Porphyromonas gingivalis/patogenicidade Treponema denticola/patogenicidade [Mh] Termos MeSH secundário: Periodontite Agressiva/microbiologia Periodontite Agressiva/fisiopatologia Carga Bacteriana Doença da Artéria Coronariana/microbiologia Doença da Artéria Coronariana/fisiopatologia Seres Humanos Fatores de Risco Estados Unidos [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171020 [Lr] Data última revisão: 171020 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161201 [St] Status: MEDLINE [do] DOI: 10.1136/postgradmedj-2016-134279

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[PMID]: 27697692 [Au] Autor: Jun HK; Jung YJ; Choi BK [Ad] Endereço: Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, South Korea. Electronic address: enqn09@snu.ac.kr. [Ti] Título: Treponema denticola, Porphyromonas gingivalis, and Tannerella forsythia induce cell death and release of endogenous danger signals. [So] Source: Arch Oral Biol;73:72-78, 2017 Jan. [Is] ISSN: 1879-1506 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: OBJECTIVE: The aim of this study was to analyze whether periodontopathogens induced inflammatory cell death and the release of diverse endogenous danger molecules in THP-1-derived macrophages. METHODS: The macrophages were treated with Treponema denticola, Porphyromonas gingivalis, and Tannerella forsythia. Activation of caspase-1 and caspase-4 was detected by Western blotting. Cell death of bacteria-stimulated macrophages was examined using a lactate dehydrogenase (LDH) assay and propidium iodide (PI)/annexin V (AV) staining. Levels of endogenous danger signals, including adenosine triphosphate (ATP), uric acid, heat shock protein 60 (HSP60), high-mobility group box protein 1 (HMGB1), and fibronectin in the culture supernatants were determined using an ATP bioluminescence assay kit, a uric acid assay kit, and Western blotting, respectively. RESULTS: T. denticola, P. gingivalis, and T. forsythia induced activation of caspase-1 and caspase-4. The LDH assay and PI/AV staining showed that all three pathogens induced pyroptotic cell death. All three bacteria induced release of ATP, which is an important ligand for inflammasome activation; the increase in ATP ultimately leads to caspase-1 activation. T. denticola induced release of HSP60 and fibronectin, while T. forsythia induced release of HMGB1 in addition to HSP60 and fibronectin. None of the endogenous molecules except for fibronectin were detected in P. gingivalis-infected cells, possibly due to degradation of these factors by the proteolytic activity of the bacteria. Interestingly, P. gingivalis induced uric acid release. CONCLUSION: Inflammatory cell death and endogenous danger molecules released from cells infected with periodontopathogens may play critical roles in the pathogenesis and progression of periodontitis by augmenting immune and inflammatory responses. [Mh] Termos MeSH primário: Morte Celular/fisiologia Periodontite/microbiologia Porphyromonas gingivalis/patogenicidade Tannerella forsythia/patogenicidade Treponema denticola/patogenicidade [Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo Western Blotting Caspase 1/metabolismo Caspases Iniciadoras/metabolismo Células Cultivadas Chaperonina 60/metabolismo Fibronectinas/metabolismo Citometria de Fluxo Proteínas HMGB/metabolismo Seres Humanos Macrófagos Porphyromonas gingivalis/enzimologia Transdução de Sinais Tannerella forsythia/enzimologia Treponema denticola/enzimologia Ácido Úrico/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Chaperonin 60); 0 (Fibronectins); 0 (HMGB Proteins); 268B43MJ25 (Uric Acid); 8L70Q75FXE (Adenosine Triphosphate); EC 3.4.22.- (CASP4 protein, human); EC 3.4.22.- (Caspases, Initiator); EC 3.4.22.36 (Caspase 1) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170324 [Lr] Data última revisão: 170324 [Sb] Subgrupo de revista: D; IM [Da] Data de entrada para processamento: 161005 [St] Status: MEDLINE

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[PMID]: 27696564 [Au] Autor: Kurniyati K; Kelly JF; Vinogradov E; Robotham A; Tu Y; Wang J; Liu J; Logan SM; Li C [Ad] Endereço: Department of Oral Biology, The State University of New York at Buffalo, New York, NY, 14214, USA. [Ti] Título: A novel glycan modifies the flagellar filament proteins of the oral bacterium Treponema denticola. [So] Source: Mol Microbiol;103(1):67-85, 2017 01. [Is] ISSN: 1365-2958 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: While protein glycosylation has been reported in several spirochetes including the syphilis bacterium Treponema pallidum and Lyme disease pathogen Borrelia burgdorferi, the pertinent glycan structures and their roles remain uncharacterized. Herein, a novel glycan with an unusual chemical composition and structure in the oral spirochete Treponema denticola, a keystone pathogen of periodontitis was reported. The identified glycan of mass 450.2 Da is composed of a monoacetylated nonulosonic acid (Non) with a novel extended N7 acyl modification, a 2-methoxy-4,5,6-trihydroxy-hexanoyl residue in which the Non has a pseudaminic acid configuration (L-glycero-L-manno) and is ß-linked to serine or threonine residues. This novel glycan modifies the flagellin proteins (FlaBs) of T. denticola by O-linkage at multiple sites near the D1 domain, a highly conserved region of bacterial flagellins that interact with Toll-like receptor 5. Furthermore, mutagenesis studies demonstrate that the glycosylation plays an essential role in the flagellar assembly and motility of T. denticola. To our knowledge, this novel glycan and its unique modification sites have not been reported previously in any bacteria. [Mh] Termos MeSH primário: Polissacarídeos/química Polissacarídeos/metabolismo Treponema denticola/metabolismo [Mh] Termos MeSH secundário: Sequência de Aminoácidos Proteínas de Bactérias/metabolismo Flagelos/genética Flagelos/metabolismo Flagelina/metabolismo Glicosilação Relação Estrutura-Atividade Treponema denticola/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Polysaccharides); 12777-81-0 (Flagellin) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161004 [St] Status: MEDLINE [do] DOI: 10.1111/mmi.13544

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[PMID]: 27224005 [Au] Autor: Chukkapalli SS; Velsko IM; Rivera-Kweh MF; Larjava H; Lucas AR; Kesavalu L [Ad] Endereço: Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. [Ti] Título: Global TLR2 and 4 deficiency in mice impacts bone resorption, inflammatory markers and atherosclerosis to polymicrobial infection. [So] Source: Mol Oral Microbiol;32(3):211-225, 2017 Jun. [Is] ISSN: 2041-1014 [Cp] País de publicação: Denmark [La] Idioma: eng [Ab] Resumo: Toll-like-receptors (TLRs) play a significant role in the generation of a specific innate immune response against invading pathogens. TLR2 and TLR4 signaling contributes to infection-induced inflammation in periodontal disease (PD) and atherosclerosis. Observational studies point towards a relationship between PD and atherosclerosis, but the role of TLR2 and TLR4 in the recognition of multiple oral pathogens and their modulation of host response leading to atherosclerosis are not clear. We evaluated the role of TLR2 and TLR4 signaling in the induction of both PD and atherosclerosis in TLR2 and TLR4 mice to polymicrobial infection with periodontal pathogens Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Polybacterial infections have established gingival colonization in TLR2 and TLR4 mice and induction of a pathogen-specific immunoglobulin G immune response. But TLR deficiency dampened accelerated alveolar bone resorption and intrabony defects, indicating a central role in infection-induced PD. Periodontal bacteria disseminated from gingival tissue to the heart and aorta through intravascular dissemination; however, there was no increase in atherosclerosis progression in the aortic arch. Polybacterial infection does not alter levels of serum risk factors such as oxidized low-density lipoprotein, nitric oxide, and lipid fractions in both mice. Polymicrobial-infected TLR2 mice demonstrated significant levels (P < 0.05 to P < 0.01) of T helper type 2 [transforming growth factor-ß , macrophage inflammatory protein-3α, interleukin-13 (IL-13)] and T helper type 17 (IL-17, IL-21, IL-22, IL-23) splenic T-cell cytokine responses. Increased heat-shock protein expression, hspa1a for Hsp 70, was observed for both TLR2 and TLR4 mice. This study supports a role for TLR2 and TLR4 in PD and atherosclerosis, corroborating an intricate association between two inflammatory diseases. [Mh] Termos MeSH primário: Aterosclerose/fisiopatologia Reabsorção Óssea/fisiopatologia Coinfecção/imunologia Inflamação/fisiopatologia Receptor 2 Toll-Like/deficiência Receptor 4 Toll-Like/deficiência [Mh] Termos MeSH secundário: Animais Aterosclerose/etiologia Aterosclerose/imunologia Reabsorção Óssea/etiologia Reabsorção Óssea/imunologia Coinfecção/microbiologia Citocinas/sangue Fusobacterium nucleatum/imunologia Proteínas de Choque Térmico/sangue Inflamação/etiologia Inflamação/imunologia Lipoproteínas LDL/sangue Camundongos Óxido Nítrico/sangue Doenças Periodontais/microbiologia Porphyromonas gingivalis/imunologia Tannerella forsythia/imunologia Células Th2/imunologia Receptor 2 Toll-Like/genética Receptor 2 Toll-Like/imunologia Receptor 4 Toll-Like/genética Receptor 4 Toll-Like/imunologia Treponema denticola/imunologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cytokines); 0 (Heat-Shock Proteins); 0 (Lipoproteins, LDL); 0 (Tlr2 protein, mouse); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 2); 0 (Toll-Like Receptor 4); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171109 [Lr] Data última revisão: 171109 [Sb] Subgrupo de revista: D [Da] Data de entrada para processamento: 160526 [St] Status: MEDLINE [do] DOI: 10.1111/omi.12165

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[PMID]: 27966504 [Au] Autor: Raj KR; Musalaiah S; Nagasri M; Kumar PA; Reddy PI; Greeshma M [Ad] Endereço: Department of Periodontics, St. Joseph Dental College and Hospital, Duggirala, Eluru, Andhra Pradesh, India. [Ti] Título: Evaluation of efficacy of photodynamic therapy as an adjunct to nonsurgical periodontal therapy in treatment of chronic periodontitis patients: A clinico-microbiological study. [So] Source: Indian J Dent Res;27(5):483-487, 2016 Sep-Oct. [Is] ISSN: 1998-3603 [Cp] País de publicação: India [La] Idioma: eng [Ab] Resumo: BACKGROUND AND OBJECTIVES: Photodynamic therapy (PDT) is a local noninvasive treatment modality without side effects caused by antibiotics. The aim of this study was to evaluate the efficacy of adjunctive use of PDT with scaling and root planing as compared with SRP alone in the treatment of chronic periodontitis. SUBJECTS AND METHODS: Twenty participants with chronic periodontitis having probing pocket depths (PDs) of ≥5 mm were selected for the study. Patients were randomly divided into control group and test group with ten patients in each group. Full-mouth SRP was performed in both the groups, followed by PDT in test group. Assessment of plaque index (PI), gingival index (GI), PD, and clinical attachment level (CAL) was done at baseline and after 3 months. Microbiological assessment of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola was done by polymerase chain reaction (PCR) at baseline and 3 months after the therapy. RESULTS: There was a significant reduction in PI, GI, PD, CAL, and microbiologic parameters in test group, following SRP and PDT, when compared with SRP alone in control group. CONCLUSION: PDT in conjunction with SRP has shown additional improvement in periodontal parameters when compared to SRP alone and has a beneficial effect in chronic periodontitis patients. [Mh] Termos MeSH primário: Periodontite Crônica/terapia Fotoquimioterapia/métodos [Mh] Termos MeSH secundário: Adulto Periodontite Crônica/microbiologia Terapia Combinada Índice de Placa Dentária Raspagem Dentária/métodos Feminino Seres Humanos Masculino Meia-Idade Reação em Cadeia da Polimerase Porphyromonas gingivalis Aplainamento Radicular/métodos Tannerella forsythia Resultado do Tratamento Treponema denticola [Pt] Tipo de publicação: JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171012 [Lr] Data última revisão: 171012 [Sb] Subgrupo de revista: D [Da] Data de entrada para processamento: 161215 [St] Status: MEDLINE [do] DOI: 10.4103/0970-9290.195622

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