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Pesquisa : B03.440.450.009 [Categoria DeCS]
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  1 / 1016 MEDLINE  
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[PMID]:29381705
[Au] Autor:Nag A; St John PC; Crowley MF; Bomble YJ
[Ad] Endereço:Computational Science Center, National Renewable Energy Laboratory, Golden, Colorado, United States of America.
[Ti] Título:Prediction of reaction knockouts to maximize succinate production by Actinobacillus succinogenes.
[So] Source:PLoS One;13(1):e0189144, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Succinate is a precursor of multiple commodity chemicals and bio-based succinate production is an active area of industrial bioengineering research. One of the most important microbial strains for bio-based production of succinate is the capnophilic gram-negative bacterium Actinobacillus succinogenes, which naturally produces succinate by a mixed-acid fermentative pathway. To engineer A. succinogenes to improve succinate yields during mixed acid fermentation, it is important to have a detailed understanding of the metabolic flux distribution in A. succinogenes when grown in suitable media. To this end, we have developed a detailed stoichiometric model of the A. succinogenes central metabolism that includes the biosynthetic pathways for the main components of biomass-namely glycogen, amino acids, DNA, RNA, lipids and UDP-N-Acetyl-α-D-glucosamine. We have validated our model by comparing model predictions generated via flux balance analysis with experimental results on mixed acid fermentation. Moreover, we have used the model to predict single and double reaction knockouts to maximize succinate production while maintaining growth viability. According to our model, succinate production can be maximized by knocking out either of the reactions catalyzed by the PTA (phosphate acetyltransferase) and ACK (acetyl kinase) enzymes, whereas the double knockouts of PEPCK (phosphoenolpyruvate carboxykinase) and PTA or PEPCK and ACK enzymes are the most effective in increasing succinate production.
[Mh] Termos MeSH primário: Actinobacillus/metabolismo
Técnicas de Silenciamento de Genes
Ácido Succínico/metabolismo
[Mh] Termos MeSH secundário: Actinobacillus/enzimologia
Actinobacillus/genética
Biomassa
Meios de Cultura
Fermentação
Modelos Biológicos
Fosfato Acetiltransferase/genética
Fosfato Acetiltransferase/metabolismo
Fosfoenolpiruvato Carboxiquinase (ATP)/genética
Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); AB6MNQ6J6L (Succinic Acid); EC 2.3.1.8 (Phosphate Acetyltransferase); EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP))
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189144


  2 / 1016 MEDLINE  
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[PMID]:28433910
[Au] Autor:Alexandri M; Papapostolou H; Stragier L; Verstraete W; Papanikolaou S; Koutinas AA
[Ad] Endereço:Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
[Ti] Título:Succinic acid production by immobilized cultures using spent sulphite liquor as fermentation medium.
[So] Source:Bioresour Technol;238:214-222, 2017 Aug.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spent sulphite liquor (SSL) was used as carbon source for the production of succinic acid using immobilized cultures of Actinobacillus succinogenes and Basfia succiniciproducens on two different supports, delignified cellulosic material (DCM) and alginate beads. Fed-batch immobilized cultures with A. succinogenes in alginates resulted in higher sugar to succinic acid conversion yield (0.81g/g) than the respective yield achieved (0.65g/g) when DCM immobilized cultures were used. The final succinic acid concentration and yield achieved in fed-batch with immobilized cultures of B. succiniciproducens in alginates (45g/L and 0.66g/g) were higher than A. succinogenes immobilized cultures (35.4g/L and 0.61g/g) using nano-filtrated SSL as fermentation medium. Immobilized cultures of B. succiniciproducens in alginate beads were reused in four sequential fed-batch fermentations of nano-filtrated SSL leading to the production of 64.7g of succinic acid with a yield range of 0.42-0.67g/g and productivity range of 0.29-0.65g/L/h. The immobilized cultures improved the efficiency of succinic acid production as compared to free cell cultures.
[Mh] Termos MeSH primário: Actinobacillus
Fermentação
Ácido Succínico
[Mh] Termos MeSH secundário: Reatores Biológicos
Sulfitos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfites); AB6MNQ6J6L (Succinic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE


  3 / 1016 MEDLINE  
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[PMID]:28381551
[Au] Autor:Song Q; Wu Z; Fan Y; Song W; Zhang P; Wang L; Wang F; Xu Y; Wang PG; Cheng J
[Ad] Endereço:From the State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, China and.
[Ti] Título:Production of homogeneous glycoprotein with multisite modifications by an engineered -glycosyltransferase mutant.
[So] Source:J Biol Chem;292(21):8856-8863, 2017 May 26.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Naturally occurring -glycoproteins exhibit glycoform heterogeneity with respect to -glycan sequon occupancy (macroheterogeneity) and glycan structure (microheterogeneity). However, access to well-defined glycoproteins is always important for both basic research and therapeutic purposes. As a result, there has been a substantial effort to identify and understand the catalytic properties of -glycosyltransferases, enzymes that install the first glycan on the protein chain. In this study we found that ApNGT, a newly discovered cytoplasmic -glycosyltransferase from , has strict selectivity toward the residues around the Asn of -glycosylation sequon by screening a small library of synthetic peptides. The inherent stringency was subsequently demonstrated to be closely associated with a critical residue (Gln-469) of ApNGT which we propose hinders the access of bulky residues surrounding the occupied Asn into the active site. Site-saturated mutagenesis revealed that the introduction of small hydrophobic residues at the site cannot only weaken the stringency of ApNGT but can also contribute to enormous improvement of glycosylation efficiency against both short peptides and proteins. We then employed the most efficient mutant (Q469A) other than the wild-type ApNGT to produce a homogeneous glycoprotein carrying multiple (up to 10) -glycans, demonstrating that this construct is a promising biocatalyst for potentially addressing the issue of macroheterogeneity in glycoprotein preparation.
[Mh] Termos MeSH primário: Actinobacillus
Substituição de Aminoácidos
Proteínas de Bactérias
Glicoproteínas
Glicosiltransferases
[Mh] Termos MeSH secundário: Actinobacillus/genética
Actinobacillus/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Glicoproteínas/biossíntese
Glicoproteínas/genética
Glicosilação
Glicosiltransferases/genética
Glicosiltransferases/metabolismo
Mutação de Sentido Incorreto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Glycoproteins); EC 2.4.- (Glycosyltransferases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.777383


  4 / 1016 MEDLINE  
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[PMID]:28285227
[Au] Autor:Thuy NT; Kongkaew A; Flood A; Boontawan A
[Ad] Endereço:School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand.
[Ti] Título:Fermentation and crystallization of succinic acid from Actinobacillus succinogenes ATCC55618 using fresh cassava root as the main substrate.
[So] Source:Bioresour Technol;233:342-352, 2017 Jun.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The fermentation of succinic acid from fresh cassava root using Actinobacillus succinogenes ATCC55618, and the recovery of the product using crystallization were investigated. Fresh cassava root is an ideal succinic acid feedstock due to its low price and high starch content. Saccharification was carried out using commercially available enzymes and diammonium phosphate was used as an inexpensive nitrogen source. Different fermentation modes were compared in terms of product yield and productivity. Results for fed-batch fermentations showed that a succinic acid titer of 151.44g/L, with yield and productivity of 1.51g /g and 3.22g/L/h could be obtained. Seeded batch cooling crystallization was investigated after pre-treatment using nanofiltration. A succinic acid crystal purity of 99.35% with a relative crystallinity of 96.77% was obtained from high seeding experiments. These results indicated that fresh cassava roots could be an economically alternative feedstock for a high quality succinic acid production.
[Mh] Termos MeSH primário: Actinobacillus
[Mh] Termos MeSH secundário: Cristalização
Fermentação
Manihot
Ácido Succínico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
AB6MNQ6J6L (Succinic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  5 / 1016 MEDLINE  
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[PMID]:28185883
[Au] Autor:Guan J; Berlinger SA; Li X; Chao Z; Sousa E Silva V; Banta S; West AC
[Ad] Endereço:Department of Chemical Engineering, Columbia University, 500 W 120th Street, New York, NY 10027, USA.
[Ti] Título:Development of reactor configurations for an electrofuels platform utilizing genetically modified iron oxidizing bacteria for the reduction of CO to biochemicals.
[So] Source:J Biotechnol;245:21-27, 2017 Mar 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Electrofuels processes are potentially promising platforms for biochemical production from CO using renewable energy. When coupled to solar panels, this approach could avoid the inefficiencies of photosynthesis and there is no competition with food agriculture. In addition, these systems could potentially be used to store intermittent or stranded electricity generated from other renewable sources. Here we develop reactor configurations for continuous electrofuels processes to convert electricity and CO to isobutyric acid (IBA) using genetically modified (GM) chemolithoautotrophic Acidithiobacillus ferrooxidans. These cells oxidize ferrous iron which can be electrochemically reduced. During two weeks of cultivation on ferrous iron, stable cell growth and continuous IBA production from CO were achieved in a process where media was circulated between electrochemical and biochemical rectors. An alternative process with an additional electrochemical cell for accelerated ferrous production was developed, and this system achieved an almost three-fold increase in steady state cell densities, and an almost 4-fold increase in the ferrous iron oxidation rate. Combined, this led to an almost 8-fold increase in the steady state volumetric productivity of IBA up to 0.063±0.012mg/L/h, without a decline in energy efficiency from previous work. Continued development of reactor configurations which can increase the delivery of energy to the genetically modified cells will be required to increase product titers and volumetric productivities.
[Mh] Termos MeSH primário: Actinobacillus
Fontes de Energia Bioelétrica/microbiologia
Reatores Biológicos/microbiologia
Dióxido de Carbono/metabolismo
Compostos Ferrosos/metabolismo
Organismos Geneticamente Modificados
[Mh] Termos MeSH secundário: Actinobacillus/genética
Actinobacillus/metabolismo
Organismos Geneticamente Modificados/genética
Organismos Geneticamente Modificados/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ferrous Compounds); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  6 / 1016 MEDLINE  
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[PMID]:28110662
[Au] Autor:Chen PC; Zheng P; Ye XY; Ji F
[Ad] Endereço:The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
[Ti] Título:Preparation of A. succinogenes immobilized microfiber membrane for repeated production of succinic acid.
[So] Source:Enzyme Microb Technol;98:34-42, 2017 Mar.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new applicability of cell-immobilized system for biological production of target chemical was reported in this work. A. succinogenes CCTCC M2012036 was immobilized on positively charged polypropylene microfiber membrane by physical interaction and were used for converting glucose into succinic acid. Glucose consumption and succinic acid production kinetics were investigated for optimizing the operational parameters. The cell-immobilized membrane presented good reuse stability, and six cycles of fermentation without activity loss were realized with an average succinic acid yield of 0.83g/g. Importantly, a biofilm was formed which favored the production of succinic acid. A microfiber membrane bioreactor was further constructed with the cell-immobilized membrane to perform fermentation in a larger scale, and the yield and productivity of succinic acid were 0.82g/g and 1.04gL h using a fed-batch strategy. By combining mesoporous support with biotechnological techniques, this work offered a prospect of adopting reusable cells feasible for industry.
[Mh] Termos MeSH primário: Actinobacillus/metabolismo
Reatores Biológicos/microbiologia
Ácido Succínico/metabolismo
[Mh] Termos MeSH secundário: Biofilmes
Biotecnologia
Células Imobilizadas/metabolismo
Reutilização de Equipamento
Fermentação
Glucose/metabolismo
Cinética
Membranas Artificiais
Polipropilenos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Polypropylenes); AB6MNQ6J6L (Succinic Acid); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


  7 / 1016 MEDLINE  
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[PMID]:27623815
[Au] Autor:Zhu W; Li Q; Dai N
[Ad] Endereço:Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
[Ti] Título:CO Biofixation of Actinobacillus succinogenes Through Novel Amine-Functionalized Polystyrene Microsphere Materials.
[So] Source:Appl Biochem Biotechnol;181(2):584-592, 2017 Feb.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CO -derived succinate production was enhanced by Actinobacillus succinogenes through polystyrene (PSt) microsphere materials for CO adsorption in bioreactor, and the adhesion forces between A. succinogenes bacteria and PSt materials were characterized. Synthesized uniformly sized and highly cross-linked PSt microspheres had high specific surface areas. After modification with amine functional groups, the novel amine-functionalized PSt microspheres exhibited a high adsorption capacity of 25.3 mg CO /g materials. After addition with the functionalized microspheres into the culture broth, CO supply to the cells increased. Succinate production by A. succinogenes can be enhanced from 29.6 to 48.1 g L . Moreover, the characterization of interaction forces between A. succinogenes cells and the microspheres indicated that the maximal adhesive force was about 250 pN. The amine-functionalized PSt microspheres can adsorb a large amount of CO and be employed for A. succinogenes anaerobic cultivation in bioreactor for high-efficiency production of CO -derived succinate.
[Mh] Termos MeSH primário: Actinobacillus/metabolismo
Aminas/sangue
Reatores Biológicos/microbiologia
Dióxido de Carbono/metabolismo
Poliestirenos/sangue
Ácido Succínico/metabolismo
[Mh] Termos MeSH secundário: Absorção Fisico-Química
Poluentes Atmosféricos/química
Poluentes Atmosféricos/isolamento & purificação
Poluentes Atmosféricos/metabolismo
Biodegradação Ambiental
Dióxido de Carbono/química
Dióxido de Carbono/isolamento & purificação
Microesferas
Ácido Succínico/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Amines); 0 (Polystyrenes); 142M471B3J (Carbon Dioxide); AB6MNQ6J6L (Succinic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160915
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2233-2


  8 / 1016 MEDLINE  
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[PMID]:27631960
[Au] Autor:Bradfield MF; Nicol W
[Ad] Endereço:Department of Chemical Engineering, University of Pretoria, Lynnwood Road, Hatfield, Private Bag X20, Pretoria, 0002, South Africa.
[Ti] Título:The pentose phosphate pathway leads to enhanced succinic acid flux in biofilms of wild-type Actinobacillus succinogenes.
[So] Source:Appl Microbiol Biotechnol;100(22):9641-9652, 2016 Nov.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four different dilution rates. Glucose-6-phosphate dehydrogenase assays were performed on cell extracts derived from in situ removal of biofilm at each steady state. The results of the assays were coupled to a kinetic model that revealed an increase in oxidative pentose phosphate pathway (OPPP) flux relative to total substrate flux with increasing SA titre, for both substrates. Furthermore, applying metabolite concentration data to metabolic flux models that include the OPPP revealed similar flux relationships to those observed in the experimental kinetic analysis. A relative increase in OPPP flux produces additional reduction power that enables increased flux through the reductive branch of the TCA cycle, leading to increased SA yields, reduced by-product formation and complete closure of the overall redox balance.
[Mh] Termos MeSH primário: Actinobacillus/fisiologia
Biofilmes
Via de Pentose Fosfato
Ácido Succínico/metabolismo
[Mh] Termos MeSH secundário: Actinobacillus/metabolismo
Fermentação
Glucose/metabolismo
Glucosefosfato Desidrogenase/análise
Análise do Fluxo Metabólico
Xilose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
A1TA934AKO (Xylose); AB6MNQ6J6L (Succinic Acid); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE


  9 / 1016 MEDLINE  
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[PMID]:27520031
[Au] Autor:Meng J; Wang B; Liu D; Chen T; Wang Z; Zhao X
[Ad] Endereço:Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
[Ti] Título:High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli.
[So] Source:Microb Cell Fact;15(1):141, 2016 Aug 12.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Succinate has been identified by the U.S. Department of Energy as one of the top 12 building block chemicals, which can be used as a specialty chemical in the agricultural, food, and pharmaceutical industries. Escherichia coli are now one of the most important succinate producing candidates. However, the stoichiometric maximum succinate yield under anaerobic conditions through the reductive branch of the TCA cycle is restricted by NADH supply in E. coli. RESULTS: In the present work, we report a rational approach to increase succinate yield by regulating NADH supply via pentose phosphate (PP) pathway and enhancing flux towards succinate. The deregulated genes zwf243 (encoding glucose-6-phosphate dehydrogenase) and gnd361 (encoding 6-phosphogluconate dehydrogenase) involved in NADPH generation from Corynebacterium glutamicum were firstly introduced into E. coli for succinate production. Co-expression of beneficial mutated dehydrogenases, which removed feedback inhibition in the oxidative part of the PP pathway, increased succinate yield from 1.01 to 1.16 mol/mol glucose. Three critical genes, pgl (encoding 6-phosphogluconolactonase), tktA (encoding transketolase) and talB (encoding transaldolase) were then overexpressed to redirect more carbon flux towards PP pathway and further improved succinate yield to 1.21 mol/mol glucose. Furthermore, introducing Actinobacillus succinogenes pepck (encoding phosphoenolpyruvate carboxykinase) together with overexpressing sthA (encoding soluble transhydrogenase), further increased succinate yield to 1.31 mol/mol glucose. In addition, removing byproduct formation through inactivating acetate formation genes ackA-pta and heterogenously expressing pyc (encoding pyruvate carboxylase) from C. glutamicum led to improved succinate yield to 1.4 mol/mol glucose. Finally, synchronously overexpressing dcuB and dcuC encoding succinate exporters enhanced succinate yield to 1.54 mol/mol glucose, representing 52 % increase relative to the parent strain and amounting to 90 % of the strain-specific stoichiometric maximum (1.714 mol/mol glucose). CONCLUSIONS: It's the first time to rationally regulate pentose phosphate pathway to improve NADH supply for succinate synthesis in E. coli. 90 % of stoichiometric maximum succinate yield was achieved by combining further flux increase towards succinate and engineering its export. Regulation of NADH supply via PP pathway is therefore recommended for the production of products that are NADH-demanding in E. coli.
[Mh] Termos MeSH primário: Proteínas de Escherichia coli/genética
Escherichia coli/genética
Escherichia coli/metabolismo
Redes e Vias Metabólicas/genética
NADP/metabolismo
Via de Pentose Fosfato/genética
Ácido Succínico/metabolismo
[Mh] Termos MeSH secundário: Actinobacillus/genética
Anaerobiose
Ciclo do Carbono
Hidrolases de Éster Carboxílico/genética
Hidrolases de Éster Carboxílico/metabolismo
Corynebacterium glutamicum/genética
Regulação Bacteriana da Expressão Gênica
Glucose/metabolismo
Glucosefosfato Desidrogenase/genética
Mutação
Fosfogluconato Desidrogenase/genética
Piruvato Carboxilase/genética
Piruvato Carboxilase/metabolismo
Transaldolase/genética
Transaldolase/metabolismo
Transcetolase/genética
Transcetolase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 53-59-8 (NADP); AB6MNQ6J6L (Succinic Acid); EC 1.1.1.43 (Phosphogluconate Dehydrogenase); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); EC 2.2.1.1 (Transketolase); EC 2.2.1.2 (Transaldolase); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.31 (6-phosphogluconolactonase); EC 6.4.1.1 (Pyruvate Carboxylase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-016-0536-1


  10 / 1016 MEDLINE  
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[PMID]:27394995
[Au] Autor:Carvalho M; Roca C; Reis MA
[Ad] Endereço:REQUIMTE, DQ/FCT, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal.
[Ti] Título:Improving succinic acid production by Actinobacillus succinogenes from raw industrial carob pods.
[So] Source:Bioresour Technol;218:491-7, 2016 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carob pods are an inexpensive by-product of locust bean gum industry that can be used as renewable feedstock for bio-based succinic acid. Here, for the first time, unprocessed raw carob pods were used to extract a highly enriched sugar solution, afterwards used as substrate to produce succinic acid using Actinobacillus succinogenes. Batch fermentations containing 30g/L sugars resulted in a production rate of 1.67gSA/L.h and a yield of 0.39gSA/g sugars. Taking advantage of A. succinogenes' metabolism, uncoupling cell growth from succinic acid production, a fed-batch mode was implemented to increase succinic acid yield and reduce by-products formation. This strategy resulted in a succinic acid yield of 0.94gSA/g sugars, the highest yield reported in the literature for fed-batch and continuous experiments, while maintaining by-products at residual values. Results demonstrate that raw carob pods are a highly efficient feedstock for bio-based succinic acid production.
[Mh] Termos MeSH primário: Actinobacillus/metabolismo
Carboidratos/química
Galactanos/química
Mananas/química
Gomas Vegetais/química
Ácido Succínico/química
[Mh] Termos MeSH secundário: Ácido Acético/química
Análise Custo-Benefício
Fabaceae/química
Fermentação
Tecnologia de Alimentos
Formiatos/química
Microbiologia Industrial
Cinética
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Formates); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 059QF0KO0R (Water); 0YIW783RG1 (formic acid); AB6MNQ6J6L (Succinic Acid); Q40Q9N063P (Acetic Acid); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160711
[St] Status:MEDLINE



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