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[PMID]:28458058
[Au] Autor:Miyagi K; Sano K; Hirai I
[Ad] Endereço:Laboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa, 903-0215, Japan. Electronic address: k-miyagi@med.u-ryukyu.ac.jp.
[Ti] Título:Sanitary evaluation of domestic water supply facilities with storage tanks and detection of Aeromonas, enteric and related bacteria in domestic water facilities in Okinawa Prefecture of Japan.
[So] Source:Water Res;119:171-177, 2017 08 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To provide for temporary restrictions of the public water supply system, storage tanks are commonly installed in the domestic water systems of houses and apartment buildings in Okinawa Prefecture of Japan. To learn more about the sanitary condition and management of these water supply facilities with storage tanks (hereafter called "storage tank water systems") and the extent of bacterial contamination of water from these facilities, we investigated their usage and the existence of Aeromonas, enteric and related bacteria. Verbal interviews concerning the use and management of the storage tank water systems were carried out in each randomly sampled household. A total of 54 water samples were collected for bacteriological and physicochemical examinations. Conventional methods were used for total viable count, fecal coliforms, identification of bacteria such as Aeromonas, Enterobacteriaceae and non-fermentative Gram-negative rods (NF-GNR), and measurement of residual chlorine. On Aeromonas species, tests for putative virulence factor and an identification using 16S rRNA and rpoB genes were also performed. Water from the water storage systems was reported to be consumed directly without boiling in 22 of the 54 houses (40.7%). 31 of the sampled houses had installed water storage tanks of more than 1 cubic meter (m ) per inhabitant, and in 21 of the sampled houses, the tank had never been cleaned. In all samples, the total viable count and fecal coliforms did not exceed quality levels prescribed by Japanese waterworks law. Although the quantity of bacteria detected was not high, 23 NF-GNR, 14 Enterobacteriaceae and 5 Aeromonas were isolated in 42.6%, 7.4% and 3.7% of samples respectively. One isolated A. hydrophila and four A. caviae possessed various putative virulence factors, especially A. hydrophila which had diverse putative pathogenic genes such as aer, hlyA, act, alt, ast, ser, and dam. Many bacteria were isolated when the concentration of residual chlorine was below 0.1 mg/l and the water temperature was above 20 °C. These results suggest that elevated water temperature and mismatch between tank size and water demand lead to loss of residual chlorine in tap water. Therefore, to minimize growth of aquatic bacteria such as Aeromonas spp. and Pseudomonas spp., we recommend that an appropriate size tank and/or volume of stored water is always used, and also suggest installation of some means of reducing water temperature such as shading.
[Mh] Termos MeSH primário: Aeromonas
Abastecimento de Água
[Mh] Termos MeSH secundário: Japão
RNA Ribossômico 16S
Inquéritos e Questionários
Água
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:29023605
[Au] Autor:Kobayashi H; Otsubo T; Teraoka F; Ikeda K; Seike S; Takahashi E; Okamoto K; Yoshida T; Tsuge H; Yamanaka H
[Ad] Endereço:Laboratory of Molecular Microbiological Science, Faculty of Pharmaceutical Sciences, Hiroshima International University, Hiroshima, Japan.
[Ti] Título:Involvement of the Arg566 residue of Aeromonas sobria serine protease in substrate specificity.
[So] Source:PLoS One;12(10):e0186392, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aeromonas sobria serine protease (ASP) is an extracellular serine protease secreted by the organism. Here, we identified the amino acid residue of ASP that contributes to substrate specificity by using both synthetic peptides and biological protein components. The results showed that the arginine residue at position 566 (Arg-566) of ASP, which is located in the extra occluding region of ASP close to an entrance of the catalytic cavity, is involved in the substrate specificity. A substitutional point mutation of the Arg-566 residue of ASP to Ala residue (ASP[R566A]) caused a decrease of the proteolytic efficiency for a certain substrate. In addition, ASP lost the ability to recognize the primary substrate by such a point mutation, and ASP[R566A] reacted to a wide range of synthetic substrates. It is likely that Arg-566 causes an interaction with the amino acid residue at position P3 of the substrate, which is the third amino acid residue upstream from the cleavage site. Another study using ORF2 protein, a chaperone protein of ASP, further suggested that Arg-566 could also play an important role in interaction with ORF2. We therefore conclude that the Arg-566 residue of ASP is likely responsible for the selection of substrates.
[Mh] Termos MeSH primário: Aeromonas/enzimologia
Arginina/metabolismo
Proteínas de Bactérias/metabolismo
Serina Proteases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arginina/química
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Fibrinogênio/metabolismo
Seres Humanos
Cininogênios/metabolismo
Chaperonas Moleculares/metabolismo
Mutagênese Sítio-Dirigida
Proteólise
Serina Proteases/química
Serina Proteases/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Kininogens); 0 (Molecular Chaperones); 9001-32-5 (Fibrinogen); 94ZLA3W45F (Arginine); EC 3.4.- (Serine Proteases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186392


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[PMID]:28589461
[Au] Autor:Singh SK; Ekka R; Mishra M; Mohapatra H
[Ad] Endereço:School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, HBNI, Room No. 321, 3rd floor, District-Khurda, Odisha, Jatni, 752050, India.
[Ti] Título:Association study of multiple antibiotic resistance and virulence: a strategy to assess the extent of risk posed by bacterial population in aquatic environment.
[So] Source:Environ Monit Assess;189(7):320, 2017 Jul.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study explored the association between multiple antibiotic resistance (MAR) index and virulence index to determine what percent of environmental antibiotic-resistant (eARB) bacteria could pose threat as potential pathogen. 16srRNA-based sequencing of 113 non-duplicate isolates identified majority of them to be gram negative belonging to Enterobacter, Pseudomonas, Aeromonas, Proteus, Acinetobacter, and Klebsiella. Statistical comparison of MAR indices of the abovementioned genera indicated differences in the median values among the groups (p < 0.001). Pair-wise multiple comparison by Dunn's method indicated significant difference in MAR indices (p < 0.05), based on which multiple antibiotic resistance phenotype could be ranked in the order Pseudomonas > Klebsiella = Acinetobacter > Proteus > Aeromonas > Enterobacter. Association between MAR index and virulence index revealed that 25% of isolates in the population under study posed high threat to human/animal or both; out of which 75% isolates belonged to genus Pseudomonas. Based on observations of comparative analysis of the six gram-negative genera, it could be concluded that Pseudomonas isolates from environment pose significantly high threat as potential pathogens while Enterobacter isolates posed no threat.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana/genética
Monitoramento Ambiental
Microbiologia da Água
[Mh] Termos MeSH secundário: Aeromonas
Animais
Antibacterianos/farmacologia
Seres Humanos
Testes de Sensibilidade Microbiana
Pseudomonas/isolamento & purificação
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-017-6005-4


  4 / 3253 MEDLINE  
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[PMID]:28484157
[Au] Autor:Miyamoto Y; Udaka K; Sekimoto E; Shibata H; Otsuka K; Mori K; Shigekiyo T; Ozaki S
[Ad] Endereço:Medical Education Center, Tokushima Prefectural Central Hospital.
[Ti] Título:Hematopoietic neoplasms accompanied by severe enterocolitis due to Aeromonas species.
[So] Source:Rinsho Ketsueki;58(4):303-308, 2017.
[Is] ISSN:0485-1439
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Aeromonas species are known to be a cause of diarrhea and acute enterocolitis. However, only a few cases have been reported and the pathophysiology of Aeromonas infection has not as yet been clarified. We experienced 2 cases developing severe enterocolitis during the course of hematological malignancies, specifically multiple myeloma and diffuse large B-cell lymphoma. Both patients presented with watery diarrhea that persisted for more than a week, followed by bloody diarrhea. Total colon endoscopy showed multiple ulcers on the mucosa from the sigmoid colon to the rectum, and biopsies from the ulcer revealed infiltration of neutrophils and eosinophils in the mucosa and submucosa. Aeromonas hydrophila and Aeromonas sobria were isolated from stool cultures, respectively. Treatment with oral ciprofloxacin was effective in both patients and clinical symptoms showed significant improvement. These cases raise the possibility of Aeromonas infection as a cause of severe enterocolitis and the importance of making a correct differential diagnosis and appropriate antibiotic treatment in immunocompromised patients including those with hematological malignancies.
[Mh] Termos MeSH primário: Aeromonas/isolamento & purificação
Enterocolite/microbiologia
Infecções por Bactérias Gram-Negativas/complicações
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Linfoma Difuso de Grandes Células B/complicações
Mieloma Múltiplo/complicações
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/uso terapêutico
Antineoplásicos/uso terapêutico
Ciprofloxacino/uso terapêutico
Diarreia/etiologia
Feminino
Seres Humanos
Linfoma Difuso de Grandes Células B/terapia
Masculino
Meia-Idade
Mieloma Múltiplo/tratamento farmacológico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents); 5E8K9I0O4U (Ciprofloxacin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.11406/rinketsu.58.303


  5 / 3253 MEDLINE  
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[PMID]:28432839
[Au] Autor:Imamura T; Murakami Y; Nitta H
[Ti] Título:Aeromonas sobria serine protease (ASP): a subtilisin family endopeptidase with multiple virulence activities.
[So] Source:Biol Chem;398(10):1055-1068, 2017 Sep 26.
[Is] ISSN:1437-4315
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Aeromonas sobria serine protease (ASP) is secreted from Aeromonas sobria, a pathogen causing gastroenteritis and sepsis. ASP resembles Saccharomyces cerevisiae Kex2, a member of the subtilisin family, and preferentially cleaves peptide bonds at the C-terminal side of paired basic amino acid residues; also accepting unpaired arginine at the P1 site. Unlike Kex2, however, ASP lacks an intramolecular chaperone N-terminal propeptide, instead utilizes the external chaperone ORF2 for proper folding, therefore, ASP and its homologues constitute a new subfamily in the subtilisin family. Through activation of the kallikrein/kinin system, ASP induces vascular leakage, and presumably causes edema and septic shock. ASP accelerates plasma clotting by α-thrombin generation from prothrombin, whereas it impairs plasma clottability by fibrinogen degradation, together bringing about blood coagulation disorder that occurs in disseminated intravascular coagulation, a major complication of sepsis. From complement C5 ASP liberates C5a that induces neutrophil recruitment and superoxide release, and mast cell degranulation, which are associated with pus formation, tissue injury and diarrhea, respectively. Nicked two-chain ASP also secreted from A. sobria is more resistant to inactivation by α2-macroglobulin than single-chain ASP, thereby raising virulence activities. Thus, ASP is a potent virulence factor and may participate in the pathogenesis of A. sobria infection.
[Mh] Termos MeSH primário: Aeromonas/enzimologia
Aeromonas/patogenicidade
Subtilisina/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
EC 3.4.21.62 (Subtilisin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170423
[St] Status:MEDLINE


  6 / 3253 MEDLINE  
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[PMID]:28392536
[Au] Autor:Wimalasena SHMP; Shin GW; Hossain S; Heo GJ
[Ad] Endereço:Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
[Ti] Título:Potential enterotoxicity and antimicrobial resistance pattern of Aeromonas species isolated from pet turtles and their environment.
[So] Source:J Vet Med Sci;79(5):921-926, 2017 May 23.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To investigate the potential enterotoxicity and antimicrobial resistance of aeromonads from pet turtles as a risk for human infection, one hundred and two Aeromonas spp. were isolated from the feces, skin and rearing environments of pet turtles and identified by biochemical and gyrB sequence analyses. Aeromonas enteropelogenes was the predominant species among the isolates (52.9%) followed by A. hydrophila (32.4%), A. dharkensis (5.9%), A. veronii (4.9%) and A. caviae (3.9%). Their potential enterotoxicities were evaluated by PCR assays for detecting genes encoding cytotoxic enterotoxin (act) and two cytotonic enterotoxins (alt and ast). 75.8% of A. hydrophila isolates exhibited the act /alt /ast genotype, whereas 94.4% of A. enteropelogenes isolates were determined to be act /alt /ast . In an antimicrobial susceptibility test, most isolates were susceptible to all tested antibiotics except amoxicillin, ampicillin, cephalothin, chloramphenicol and tetracycline. Non-susceptible isolates to penicillins (ampicillin and amoxicillin) and fluoroquinolones (ciprofloxacin and norfloxacin) were frequently observed among the A. enteropelogenes isolates. Few isolates were resistant to imipenem, amikacin, ceftriaxone and cefotaxime. Collectively, these results suggest that pet turtles may pose a public health risk of infection by enterotoxigenic and antimicrobial resistant Aeromonas strains.
[Mh] Termos MeSH primário: Aeromonas/patogenicidade
Antibacterianos/farmacologia
Infecções por Bactérias Gram-Negativas/veterinária
Tartarugas/microbiologia
[Mh] Termos MeSH secundário: Aeromonas/efeitos dos fármacos
Aeromonas/genética
Aeromonas hydrophila/efeitos dos fármacos
Aeromonas hydrophila/genética
Aeromonas hydrophila/patogenicidade
Animais
Antibacterianos/uso terapêutico
Enterotoxinas/genética
Microbiologia Ambiental
Fezes/microbiologia
Genes Bacterianos/genética
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Infecções por Bactérias Gram-Negativas/microbiologia
Testes de Sensibilidade Microbiana/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enterotoxins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0493


  7 / 3253 MEDLINE  
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[PMID]:28365323
[Au] Autor:Igbinosa IH; Beshiru A; Odjadjare EE; Ateba CN; Igbinosa EO
[Ad] Endereço:Department of Environmental Management and Toxicology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154 Benin City 300001, Nigeria; SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
[Ti] Título:Pathogenic potentials of Aeromonas species isolated from aquaculture and abattoir environments.
[So] Source:Microb Pathog;107:185-192, 2017 Jun.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study elucidated the presence of antibiotics resistance, virulence genes and biofilm potentials among Aeromonas species isolated from abattoir and aquaculture environments in Benin City, Nigeria. A total of 144 wastewater samples were obtained from two independent aquaculture and abattoir environments between May and October 2016. Aeromonas species were isolated on Glutamate Starch Phenol Red (GSP) agar and confirmed using API 20NE kits. Antimicrobial susceptibility profile of the isolates was carried out using standard disc diffusion assay while biofilm potentials were detected by the microtitre plate method and PCR technique was used to detect antibiotics resistance and virulence gene markers. Overall, 32 and 26 Aeromonas species were isolated from the abattoir and aquaculture environments respectively. Isolates from both environments were completely resistant (100%) to penicillin G, ertapenem and tetracycline; whereas aquaculture isolates exhibited absolute sensitivity (100%) towards cefepime. All the virulence gene markers (aerA, hlyA, alt, ast, laf, ascF-G, fla, lip, stx1, and stx2) investigated in this study (except laf) were detected in isolates from both environments. The laf genes were only detected in isolates from abattoir environments. Antibiotics resistant genes including pse, bla and class 1 integron were detected in isolates from both environments. Majority of the isolates (53/58 91.4%) from both environments; demonstrated capacity for biofilm potential. The detection of antibiotic resistance and virulence gene markers as well as biofilm forming ability in Aeromonas species isolated from aquaculture and abattoir environments raise serious public health concern worthy of further investigation.
[Mh] Termos MeSH primário: Matadouros
Aeromonas/isolamento & purificação
Aeromonas/patogenicidade
Aquicultura
[Mh] Termos MeSH secundário: Aeromonas/efeitos dos fármacos
Aeromonas/genética
Antibacterianos/farmacologia
Biofilmes/crescimento & desenvolvimento
DNA Bacteriano/genética
Farmacorresistência Bacteriana Múltipla/genética
Gelatinases/análise
Genes Bacterianos/genética
Marcadores Genéticos/genética
Integrons/genética
Testes de Sensibilidade Microbiana/métodos
Nigéria
Peptídeo Hidrolases/análise
Fenótipo
Reação em Cadeia da Polimerase
Virulência/genética
Fatores de Virulência/genética
Águas Residuais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Genetic Markers); 0 (Virulence Factors); 0 (Waste Water); EC 3.4.- (Peptide Hydrolases); EC 3.4.24.- (Gelatinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE


  8 / 3253 MEDLINE  
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[PMID]:28271852
[Au] Autor:Zhang Y; Li D; Lv J; Li Q; Kong C; Luo Y
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
[Ti] Título:Effect of cinnamon essential oil on bacterial diversity and shelf-life in vacuum-packaged common carp (Cyprinus carpio) during refrigerated storage.
[So] Source:Int J Food Microbiol;249:1-8, 2017 May 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study investigated the effect of cinnamon essential oil on the quality of vacuum-packaged common carp (Cyprinus carpio) fillets stored at 4±1°C in terms of sensory scores, physicochemical characteristics (total volatile basic nitrogen (TVB-N), biogenic amines, and color), and presence of spoilage microbiota. A total of 290,753 bacterial sequences and 162 different genera belonging to 14 phyla were observed by a high-throughput sequencing technique targeting the V3-V4 region of 16S rDNA, which showed a more comprehensive estimate of microbial diversity in carp samples compared with microbial enumeration. Before storage, Macrococcus and Aeromonas were the prevalent populations in the control samples, but cinnamon essential oil decreased the relative abundance of Macrococcus in the treated samples. Variability in the predominant microbiota in different samples during chilled storage was observed. Aeromonas followed by Lactococcus were the major contaminants in the spoiled control samples. Microbial enumeration also observed relatively higher counts of Aeromonas than other spoilage microorganisms. Compared with the control samples, cinnamon essential oil inhibited the growth of Aeromonas and Lactococcus were the predominant components in the treated samples on day 10; plate counts also revealed a relatively high level of lactic acid bacteria during refrigerated storage. However, there were no significant differences (P>0.05) in the composition of dominant microbiota between these two treatments at the end of the shelf-life. Furthermore, cinnamon essential oil treatment was more effective in inhibiting the increase of TVB-N and the accumulation of biogenic amines (especially for putrescine and cadaverine levels). Based primarily on sensory analysis, the use of cinnamon essential oil extended the shelf-life of vacuum-packaged common carp fillets by about 2days.
[Mh] Termos MeSH primário: Cadaverina/farmacologia
Conservação de Alimentos/métodos
Armazenamento de Alimentos/métodos
Óleos Voláteis/farmacologia
Putrescina/farmacologia
Alimentos Marinhos/microbiologia
[Mh] Termos MeSH secundário: Aeromonas/efeitos dos fármacos
Aeromonas/isolamento & purificação
Animais
Carpas
Cinnamomum zeylanicum/metabolismo
Microbiologia de Alimentos
Embalagem de Alimentos/métodos
Seres Humanos
Lactococcus/efeitos dos fármacos
Lactococcus/isolamento & purificação
Microbiota/efeitos dos fármacos
Tipagem Molecular
Nitrogênio/análise
RNA Ribossômico 16S/genética
Refrigeração
Staphylococcaceae/efeitos dos fármacos
Staphylococcaceae/isolamento & purificação
Vácuo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (RNA, Ribosomal, 16S); L90BEN6OLL (Cadaverine); N762921K75 (Nitrogen); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE


  9 / 3253 MEDLINE  
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[PMID]:28209550
[Au] Autor:Zepeda-Velázquez AP; Vega-Sánchez V; Ortega-Santana C; Rubio-Godoy M; de Oca-Mira DM; Soriano-Vargas E
[Ad] Endereço:Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca 50200, Mexico.
[Ti] Título:Pathogenicity of Mexican isolates of Aeromonas sp. in immersion experimentally-infected rainbow trout (Oncorhynchus mykiss, Walbaum 1792).
[So] Source:Acta Trop;169:122-124, 2017 May.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ten species of Aeromonas have been previously identified in farmed rainbow trout from Mexico. The aim of the current study was to investigate the pathogenicity of 10 Aeromonas isolates belonging to 10 different Aeromonas species in immersion experimentally-infected rainbow trout fry. Isolates of A. bestiarum, A. hydrophila, A. salmonicida, and A. veronii produced significant mortality (8.8%, 12.2%, 18.8%, and 8.8%, respectively). Isolates of A. caviae and A. sobria produced no significantly mortality (3.3% and 1.1%, respectively). No mortality was recorded in fish infected with A. allosaccharophila, A. lusitana, A. media, or A. popoffii. Microscopic lesions and bacterial reisolation were registered in liver of fish infected with the ten different Aeromonas isolates. Our results suggest that all Aeromonas species included in the study have the ability to colonize the liver. The results have confirmed that species A. bestiarum, A. hydrophila, A. salmonicida, and A. veronii affected fish as elsewhere reported. In conclusion, the variation in pathogenicity of Aeromonas isolates included in the study, emphasizes the importance of active, on-going monitoring of Aeromonas in the Mexican rainbow trout farming.
[Mh] Termos MeSH primário: Aeromonas/patogenicidade
Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Oncorhynchus mykiss/microbiologia
[Mh] Termos MeSH secundário: Animais
México
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  10 / 3253 MEDLINE  
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Alberton, Dayane
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[PMID]:28177792
[Au] Autor:Prediger KC; Surek M; Dallagassa CB; Assis FE; Piantavini MS; Souza EM; Pedrosa FO; Farah SM; Alberton D; Fadel-Picheth CM
[Ad] Endereço:a Departamento de Análises Clínicas, Universidade Federal do Paraná, Curitiba-PR, Brazil.
[Ti] Título:Utilization of carbon sources by clinical isolates of Aeromonas.
[So] Source:Can J Microbiol;63(4):359-364, 2017 Apr.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Bacteria in the genus Aeromonas are primarily aquatic organisms; however, some species can cause diseases in humans, ranging from wound infections to septicemia, of which diarrhea is the most common condition. The ability to use a variety of carbon substrates is advantageous for pathogenic bacteria. Therefore, we used Biolog GN2 microplates to analyze the ability of 103 clinical, predominantly diarrheal, isolates of Aeromonas to use various carbon sources, and we verified whether, among the substrates metabolized by these strains, there were some endogenous to the human intestine. The results indicate that Aeromonas present great diversity in the utilization of carbon sources, and that they preferentially use carbohydrates and amino acids as carbon sources. Among the carbon sources metabolized by Aeromonas in vitro, some were found to be components of intestinal mucin, including aspartic acid, glutamic acid, l-serine, galactose, N-acetyl-glucosamine, and glucose, which were used by all strains tested. Additionally, mannose, d-serine, proline, threonine, and N-acetyl-galactosamine were used by several strains. The potential to metabolize substrates endogenous to the intestine may contribute to Aeromonas' capacity to grow in and colonize the intestine. We speculate that this may help explain the ability of Aeromonas to cause diarrhea.
[Mh] Termos MeSH primário: Aeromonas/metabolismo
Carbono/metabolismo
[Mh] Termos MeSH secundário: Metabolismo dos Carboidratos
Carboidratos
Diarreia/etiologia
Seres Humanos
Intestinos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 7440-44-0 (Carbon)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2016-0526



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