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  1 / 1595 MEDLINE  
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[PMID]:29339067
[Au] Autor:Sun Y; Zhang J; Xiang J
[Ad] Endereço:College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.
[Ti] Título:Molecular characterization and function of ß-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda.
[So] Source:Gene;648:12-20, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and ß-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis. During the molting stages, EcNAG mRNA expression was well-regulated and its expression reached the highest level at the molting stage E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG was confirmed. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the expression of EcNAG was up-regulated significantly at 6 h and reached the peak at 12 h. And then, the expression began to down-regulated and came to the normal level at 72 h. It is helpful to research the relationship between the molt-related hormones and chitinlytic enzymes.
[Mh] Termos MeSH primário: Acetilglucosaminidase/genética
Proteínas de Artrópodes/genética
Muda/genética
Palaemonidae/genética
[Mh] Termos MeSH secundário: Acetilglucosaminidase/classificação
Acetilglucosaminidase/metabolismo
Aeromonas hydrophila/fisiologia
Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/classificação
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Epiderme/crescimento & desenvolvimento
Epiderme/metabolismo
Epiderme/microbiologia
Doenças dos Peixes/genética
Doenças dos Peixes/microbiologia
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento
Regulação Enzimológica da Expressão Gênica
Hepatopâncreas/crescimento & desenvolvimento
Hepatopâncreas/metabolismo
Hepatopâncreas/microbiologia
Palaemonidae/crescimento & desenvolvimento
Palaemonidae/microbiologia
Filogenia
Homologia de Sequência de Aminoácidos
Vibrio parahaemolyticus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.52 (Acetylglucosaminidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


  2 / 1595 MEDLINE  
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[PMID]:28862845
[Au] Autor:Xu Y; Wang L; Chen J; Zhao J; Fan S; Dong Y; Ha NC; Quan C
[Ad] Endereço:Department of Bioengineering, College of Life Science, Dalian Minzu University , Dalian 116600, Liaoning, China.
[Ti] Título:Structural and Functional Analyses of Periplasmic 5'-Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase from Aeromonas hydrophila.
[So] Source:Biochemistry;56(40):5347-5355, 2017 Oct 10.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Gram-negative, rod-shaped bacterium Aeromonas hydrophila has two multifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) enzymes, MtaN-1 and MtaN-2, that differ from those in other bacteria. These proteins are essential for several metabolic pathways, including biological methylation, polyamine biosynthesis, methionine recycling, and bacterial quorum sensing. To gain insight into how these two proteins function, we determined four high-resolution crystal structures of MtaN-1 in its apo form and in complex with the substrates S-adenosyl-l-homocysteine, 5'-methylthioadenosine, and 5'-deoxyadenosine. We found that the domain structures were generally similar, although slight differences were evident. The crystal structure demonstrates that AhMtaN-1 has an extension of the binding pocket and revealed that a tryptophan in the active site (Trp199) may play a major role in substrate binding, unlike in other MTAN proteins. Mutation of the Trp199 residue completely abolished the enzyme activity. Trp199 was identified as an active site residue that is essential for catalysis. Furthermore, biochemical characterization of AhMtaN-1 and AhMtaN-2 demonstrated that AhMtaN-1 exhibits inherent trypsin resistance that is higher than that of AhMtaN-2. Additionally, the thermally unfolded AhMtaN-2 protein is capable of refolding into active forms, whereas the thermally unfolded AhMtaN-1 protein does not have this ability. Examining the different biochemical characteristics related to the functional roles of AhMtaN-1 and AhMtaN-2 would be interesting. Indeed, the biochemical characterization of these structural features would provide a structural basis for the design of new antibiotics against A. hydrophila.
[Mh] Termos MeSH primário: Aeromonas hydrophila/citologia
Aeromonas hydrophila/enzimologia
Desoxiadenosinas/metabolismo
N-Glicosil Hidrolases/química
N-Glicosil Hidrolases/metabolismo
Periplasma/enzimologia
Tionucleosídeos/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Domínio Catalítico
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Deoxyadenosines); 0 (Thionucleosides); 634Z2VK3UQ (5'-methylthioadenosine); EC 3.2.2.- (N-Glycosyl Hydrolases); EC 3.2.2.9 (adenosylhomocysteine nucleosidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00691


  3 / 1595 MEDLINE  
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[PMID]:28820083
[Au] Autor:Dong YW; Jiang WD; Liu Y; Wu P; Jiang J; Kuang SY; Tang L; Tang WN; Zhang YA; Zhou XQ; Feng L
[Ad] Endereço:1Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,People's Republic of China.
[Ti] Título:Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-κB and target of rapamycin signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila.
[So] Source:Br J Nutr;118(2):92-108, 2017 Jul.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six iso-nitrogenous semi-purified diets containing graded levels of threonine (3·99-21·66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged with Aeromonas hydrophila for 14 d. Results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozyme activities (except in the distal intestine), acid phosphatase activities, complement 3 (C3) and C4 contents and IgM contents (except in the proximal intestine (PI)), and it down-regulated the transcript abundances of liver-expressed antimicrobial peptide (LEAP)-2A, LEAP-2B, hepcidin, IgZ, IgM and ß-defensin1 (except in the PI) (P<0·05); (2) could up-regulate intestinal pro-inflammatory cytokines TNF-α, IL-1ß, IL-6, IL-8 and IL-17D mRNA levels partly related to NF-κB signalling; (3) could down-regulate intestinal anti-inflammatory cytokine transforming growth factor (TGF)-ß1, TGF-ß2, IL-4/13A (not IL-4/13B) and IL-10 mRNA levels partly by target of rapamycin signalling. Finally, on the basis of the specific growth rate, against the enteritis morbidity and IgM contents, the optimum threonine requirements were estimated to be 14·53 g threonine/kg diet (4·48 g threonine/100 g protein), 15.05 g threonine/kg diet (4·64 g threonine/100 g protein) and 15·17 g threonine/kg diet (4·68 g threonine/100 g protein), respectively.
[Mh] Termos MeSH primário: Carpas/microbiologia
Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Intestinos/imunologia
Serina-Treonina Quinases TOR/metabolismo
Treonina/deficiência
[Mh] Termos MeSH secundário: Aeromonas hydrophila
Animais
Peptídeos Catiônicos Antimicrobianos/genética
Proteínas Sanguíneas
Carpas/imunologia
Citocinas/genética
Citocinas/metabolismo
Dieta/veterinária
Regulação para Baixo/efeitos dos fármacos
Enterite/veterinária
Doenças dos Peixes/imunologia
Proteínas de Peixes/genética
Hepcidinas
Imunoglobulina M
Intestinos/enzimologia
Muramidase/metabolismo
NF-kappa B/metabolismo
Transdução de Sinais/fisiologia
Treonina/administração & dosagem
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Blood Proteins); 0 (Cytokines); 0 (Fish Proteins); 0 (Hepcidins); 0 (Immunoglobulin M); 0 (NF-kappa B); 0 (liver-expressed antimicrobial peptide 2, human); 2ZD004190S (Threonine); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517001830


  4 / 1595 MEDLINE  
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[PMID]:28708867
[Au] Autor:Zhu F; Yang Z; Zhang Y; Hu K; Fang W
[Ad] Endereço:National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China.
[Ti] Título:Transcriptome differences between enrofloxacin-resistant and enrofloxacin-susceptible strains of Aeromonas hydrophila.
[So] Source:PLoS One;12(7):e0179549, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enrofloxacin is the most commonly used antibiotic to control diseases in aquatic animals caused by A. hydrophila. This study conducted de novo transcriptome sequencing and compared the global transcriptomes of enrofloxacin-resistant and enrofloxacin-susceptible strains. We got a total of 4,714 unigenes were assembled. Of these, 4,122 were annotated. A total of 3,280 unigenes were assigned to GO, 3,388 unigenes were classified into Cluster of Orthologous Groups of proteins (COG) using BLAST and BLAST2GO software, and 2,568 were mapped onto pathways using the Kyoto Encyclopedia of Gene and Genomes Pathway database. Furthermore, 218 unigenes were deemed to be DEGs. After enrofloxacin treatment, 135 genes were upregulated and 83 genes were downregulated. The GO terms biological process (126 genes) and metabolic process (136 genes) were the most enriched, and the terms for protein folding, response to stress, and SOS response were also significantly enriched. This study identified enrofloxacin treatment affects multiple biological functions of A. hydrophila. Enrofloxacin resistance in A. hydrophila is closely related to the reduction of intracellular drug accumulation caused by ABC transporters and increased expression of topoisomerase IV.
[Mh] Termos MeSH primário: Aeromonas hydrophila/genética
Farmacorresistência Bacteriana/genética
Fluoroquinolonas/farmacologia
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/genética
Transportadores de Cassetes de Ligação de ATP/metabolismo
Aeromonas hydrophila/efeitos dos fármacos
Aeromonas hydrophila/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Análise por Conglomerados
DNA Topoisomerase IV/genética
DNA Topoisomerase IV/metabolismo
Bases de Dados Genéticas
Regulação para Baixo/efeitos dos fármacos
Sequenciamento de Nucleotídeos em Larga Escala
RNA Bacteriano/química
RNA Bacteriano/isolamento & purificação
RNA Bacteriano/metabolismo
Análise de Sequência de RNA
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fluoroquinolones); 0 (RNA, Bacterial); 3DX3XEK1BN (enrofloxacin); EC 5.99.1.- (DNA Topoisomerase IV)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179549


  5 / 1595 MEDLINE  
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[PMID]:28685594
[Au] Autor:Patel B; Kumari S; Banerjee R; Samanta M; Das S
[Ad] Endereço:a Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science , National Institute of Technology , Rourkela , India.
[Ti] Título:Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen Aeromonas hydrophila by tannic acid, a potent quorum quencher.
[So] Source:Biofouling;33(7):580-590, 2017 Aug.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The quorum sensing (QS) phenomenon regulates a myriad of pathogenic traits in the biofilm forming fish pathogen, Aeromonas hydrophila. Blocking the QS mechanism of A. hydrophila is a novel strategy to prevent disease in fish. This study evaluated the effect of tannic acid, a QS inhibitor, on A. hydrophila-associated QS regulated phenomena. A streaking assay with Chromobacterium violaceum (CVO26) reported the presence of N-acyl homoserine lactone (AHL) in A. hydrophila, which was confirmed by HPLC and GC-MS analysis. Tannic acid-treated A. hydrophila showed a considerable reduction in violacein production, blood haemolysis activity and the pattern of swarming motility. Biofilm formation was significantly reduced (p < 0.001) (up to 95%), after tannic acid treatment for 48 h. Analysis by qRT-PCR revealed significant downregulation (p < 0.001) of AhyI and AhyR transcripts in A. hydrophila after tannic acid treatment. Co-stimulation of Catla catla with A. hydrophila and tannic acid attenuated pathogen-induced skin haemorrhages and increased the relative survival rate up to 86.6%. The study provides a mechanistic basis of tannic acid as a QS blocker and indicates its therapeutic potential against A. hydrophila-induced pathogenesis.
[Mh] Termos MeSH primário: Aeromonas hydrophila/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Cyprinidae/microbiologia
Percepção de Quorum/efeitos dos fármacos
Taninos/farmacologia
[Mh] Termos MeSH secundário: 4-Butirolactona/análogos & derivados
4-Butirolactona/metabolismo
Aeromonas hydrophila/genética
Aeromonas hydrophila/patogenicidade
Aeromonas hydrophila/fisiologia
Animais
Proteínas de Bactérias/genética
Chromobacterium/fisiologia
Relação Dose-Resposta a Droga
Regulação para Baixo
Virulência/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Tannins); 1192-20-7 (homoserine lactone); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2017.1336619


  6 / 1595 MEDLINE  
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[PMID]:28505394
[Au] Autor:Sevaille L; Gavara L; Bebrone C; De Luca F; Nauton L; Achard M; Mercuri P; Tanfoni S; Borgianni L; Guyon C; Lonjon P; Turan-Zitouni G; Dzieciolowski J; Becker K; Bénard L; Condon C; Maillard L; Martinez J; Frère JM; Dideberg O; Galleni M; Docquier JD; Hernandez JF
[Ad] Endereço:Institut des Biomolécules Max Mousseron, UMR5247 CNRS, Université de Montpellier, ENSCM, Faculté de Pharmacie, 15 avenue Charles Flahault, 34093, Montpellier cedex 5, France.
[Ti] Título:1,2,4-Triazole-3-thione Compounds as Inhibitors of Dizinc Metallo-ß-lactamases.
[So] Source:ChemMedChem;12(12):972-985, 2017 Jun 21.
[Is] ISSN:1860-7187
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Metallo-ß-lactamases (MBLs) cause resistance of Gram-negative bacteria to ß-lactam antibiotics and are of serious concern, because they can inactivate the last-resort carbapenems and because MBL inhibitors of clinical value are still lacking. We previously identified the original binding mode of 4-amino-2,4-dihydro-5-(2-methylphenyl)-3H-1,2,4-triazole-3-thione (compound IIIA) within the dizinc active site of the L1 MBL. Herein we present the crystallographic structure of a complex of L1 with the corresponding non-amino compound IIIB (1,2-dihydro-5-(2-methylphenyl)-3H-1,2,4-triazole-3-thione). Unexpectedly, the binding mode of IIIB was similar but reverse to that of IIIA. The 3 D structures suggested that the triazole-thione scaffold was suitable to bind to the catalytic site of dizinc metalloenzymes. On the basis of these results, we synthesized 54 analogues of IIIA or IIIB. Nineteen showed IC values in the micromolar range toward at least one of five representative MBLs (i.e., L1, VIM-4, VIM-2, NDM-1, and IMP-1). Five of these exhibited a significant inhibition of at least four enzymes, including NDM-1, VIM-2, and IMP-1. Active compounds mainly featured either halogen or bulky bicyclic aryl substituents. Finally, some compounds were also tested on several microbial dinuclear zinc-dependent hydrolases belonging to the MBL-fold superfamily (i.e., endonucleases and glyoxalase II) to explore their activity toward structurally similar but functionally distinct enzymes. Whereas the bacterial tRNases were not inhibited, the best IC values toward plasmodial glyoxalase II were in the 10 µm range.
[Mh] Termos MeSH primário: Tionas/farmacologia
Triazóis/farmacologia
Inibidores de beta-Lactamases/farmacologia
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Aeromonas hydrophila/enzimologia
Relação Dose-Resposta a Droga
Estrutura Molecular
Stenotrophomonas maltophilia/enzimologia
Relação Estrutura-Atividade
Tionas/síntese química
Tionas/química
Triazóis/síntese química
Triazóis/química
Inibidores de beta-Lactamases/síntese química
Inibidores de beta-Lactamases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Thiones); 0 (Triazoles); 0 (beta-Lactamase Inhibitors); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1002/cmdc.201700186


  7 / 1595 MEDLINE  
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[PMID]:28436829
[Au] Autor:Anastasiadi M; Lambert RJW
[Ad] Endereço:Cranfield University, Cranfield, Bedfordshire, UK.
[Ti] Título:Modelling the effect of combined antimicrobials: A base model for multiple-hurdles.
[So] Source:Int J Food Microbiol;252:10-17, 2017 Jul 03.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Combining antimicrobials to reduce microbial growth and to combat the potential impact of antimicrobial resistance is an important subject both in foods and in pharmaceutics. Modelling of combined treatments designed to reduce or eliminate microbial contamination in foods (microbiological predictive modelling) has become commonplace. Two main reference models are used to analyse mixtures: the Bliss Independence and the Loewe reference models (LRM). By using optical density to analyse the growth of Aeromonas hydrophila, Cronobacter sakazakii and Escherichia coli in combined NaCl/NaCl (a mock combination experiment) and combined NaCl/KCl experiments, previous models for combined antimicrobials in foods, based on the Bliss approach, were shown to be inconsistent and that models based on the LRM more applicable. The LRM was shown, however, to be valid only in the specific cases where the concentration exponents of all components in a mixture were identical. This is assured for a mock combination experiment but not for a true mixture. This, essentially, invalidates the LRM as a general reference model. A new model, based on the LRM but allowing for mixed exponents, was used to analyse the combined inhibition data, and concluded that the NaCl/KCl system gave the additive effect expected from literature studies. This study suggests the need to revise current models used to analyse combined effects.
[Mh] Termos MeSH primário: Aeromonas hydrophila/crescimento & desenvolvimento
Antibacterianos/farmacologia
Cronobacter sakazakii/crescimento & desenvolvimento
Escherichia coli/crescimento & desenvolvimento
Cloreto de Potássio/farmacologia
Cloreto de Sódio/farmacologia
[Mh] Termos MeSH secundário: Aeromonas hydrophila/efeitos dos fármacos
Cronobacter sakazakii/efeitos dos fármacos
Combinação de Medicamentos
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Modelos Teóricos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Drug Combinations); 451W47IQ8X (Sodium Chloride); 660YQ98I10 (Potassium Chloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


  8 / 1595 MEDLINE  
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[PMID]:28392536
[Au] Autor:Wimalasena SHMP; Shin GW; Hossain S; Heo GJ
[Ad] Endereço:Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
[Ti] Título:Potential enterotoxicity and antimicrobial resistance pattern of Aeromonas species isolated from pet turtles and their environment.
[So] Source:J Vet Med Sci;79(5):921-926, 2017 May 23.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To investigate the potential enterotoxicity and antimicrobial resistance of aeromonads from pet turtles as a risk for human infection, one hundred and two Aeromonas spp. were isolated from the feces, skin and rearing environments of pet turtles and identified by biochemical and gyrB sequence analyses. Aeromonas enteropelogenes was the predominant species among the isolates (52.9%) followed by A. hydrophila (32.4%), A. dharkensis (5.9%), A. veronii (4.9%) and A. caviae (3.9%). Their potential enterotoxicities were evaluated by PCR assays for detecting genes encoding cytotoxic enterotoxin (act) and two cytotonic enterotoxins (alt and ast). 75.8% of A. hydrophila isolates exhibited the act /alt /ast genotype, whereas 94.4% of A. enteropelogenes isolates were determined to be act /alt /ast . In an antimicrobial susceptibility test, most isolates were susceptible to all tested antibiotics except amoxicillin, ampicillin, cephalothin, chloramphenicol and tetracycline. Non-susceptible isolates to penicillins (ampicillin and amoxicillin) and fluoroquinolones (ciprofloxacin and norfloxacin) were frequently observed among the A. enteropelogenes isolates. Few isolates were resistant to imipenem, amikacin, ceftriaxone and cefotaxime. Collectively, these results suggest that pet turtles may pose a public health risk of infection by enterotoxigenic and antimicrobial resistant Aeromonas strains.
[Mh] Termos MeSH primário: Aeromonas/patogenicidade
Antibacterianos/farmacologia
Infecções por Bactérias Gram-Negativas/veterinária
Tartarugas/microbiologia
[Mh] Termos MeSH secundário: Aeromonas/efeitos dos fármacos
Aeromonas/genética
Aeromonas hydrophila/efeitos dos fármacos
Aeromonas hydrophila/genética
Aeromonas hydrophila/patogenicidade
Animais
Antibacterianos/uso terapêutico
Enterotoxinas/genética
Microbiologia Ambiental
Fezes/microbiologia
Genes Bacterianos/genética
Infecções por Bactérias Gram-Negativas/tratamento farmacológico
Infecções por Bactérias Gram-Negativas/microbiologia
Testes de Sensibilidade Microbiana/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enterotoxins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0493


  9 / 1595 MEDLINE  
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[PMID]:28363588
[Au] Autor:Safari O; Paolucci M; Motlagh HA
[Ad] Endereço:Department of Fisheries, Faculty of Natural Resources and Environment, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address: omidsafari@um.ac.ir.
[Ti] Título:Effects of synbiotics on immunity and disease resistance of narrow-clawed crayfish, Astacus leptodactylus leptodactylus (Eschscholtz, 1823).
[So] Source:Fish Shellfish Immunol;64:392-400, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the effects of prebiotics (mannanoligosaccharide and xylooligosaccharide), probiotics (Enterococcus faecalis and Pediococcus acidilactici) and synbiotics for 126 days on the immune responses, hemolymph indices, antioxidant enzymes, and biological responses after a 48-hour Aeromonas hydrophila exposure of sub-adult crayfish (11.45 ± 1.87 g). Most antibacterial activities were observed in the shell mucus of crayfish fed a diet containing xylooligosaccharide + E. faecalis and mannanoligosaccharide + Pediococcus acidilactici against Nocardia brasilience and Vibrio harveyi (p < 0.05). Feeding crayfish a xylooligosaccharide + E. faecalis diet increased protein levels and the activities of alkaline phosphatase and lysozyme in the shell mucus after the feeding trial and 48 h after the A. hydrophila-injection challenge (p < 0.05). The highest ratio of the lactobacillus count to the total viable count was observed in synbiotic diets (p < 0.05). Feeding crayfish a xylooligosaccharide + E. faecalis diet increased the growth rate and the resistance to the A. hydrophila-injection challenge (p < 0.05). These results revealed that feeding crayfish with synbiotic diets was more effective than a single administration with prebiotics and probiotics.
[Mh] Termos MeSH primário: Aeromonas hydrophila/fisiologia
Antioxidantes/metabolismo
Astacoidea/efeitos dos fármacos
Imunidade Inata
Prebióticos/análise
Simbióticos/análise
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Astacoidea/imunologia
Astacoidea/microbiologia
Dieta
Hemolinfa/metabolismo
Probióticos/farmacologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Prebiotics)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170402
[St] Status:MEDLINE


  10 / 1595 MEDLINE  
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[PMID]:28279793
[Au] Autor:Feng J; Lin P; Guo S; Jia Y; Wang Y; Zadlock F; Zhang Z
[Ad] Endereço:College of Fisheries, Jimei University, Xiamen 361021, Fujian Province, China; Engineer Research Center of Eel Modern Industry Technology, Ministry of Education, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, China. Electronic address: 2394417698@qq.com
[Ti] Título:Identification and characterization of a novel conserved 46 kD maltoporin of Aeromonas hydrophila as a versatile vaccine candidate in European eel (Anguilla anguilla).
[So] Source:Fish Shellfish Immunol;64:93-103, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:European eel (Anguilla anguilla) is a crucial economic fish that has been plagued by Aeromonas hydrophila infections for many years. Vaccines that are cross-protective against multiple serotypes could provide an effective control against A. hydrophila-mediated diseases. The outer membrane proteins (OMPs) are highly immunogenic and capable of eliciting protective immune responses. This study reports the identification of a novel 46 kD maltoporin that is a conserved protective antigen for different serotypes of A. hydrophila. First, this study purified OMPs from the strains of A. hydrophila B10, B11, B12, B15, B19, and B20. Western blot analysis revealed that the 46 kD maltoporin of B11 could be strongly reacted with all the specific European eel antisera against the above OMPs from different serotypes A. hydrophila. Cloning and sequencing of the maltoporin revealed that it contains an open reading frame (ORF) of 1281 nucleotides encoding 426 amino acids. Further sequence alignment analysis using the NCBI Conserved Domain Database (CDD) along with performing three-dimensional structure analysis showed that this protein belongs to maltoporin family. Three different study groups of European eels were intraperitoneal injected with one of the following conditions: phosphate-buffered saline (PBS group), formaline-killed-whole-cell (FKC) of A. hydrophila (FKC group) or with the recombinant maltoporin (OMP group) to analyze the immunogenicity of the recombinant maltoporin purified by nickel chelate affinity chromatography. On 14, 21, 28 and 42 days post-vaccination respectively, proliferation of the whole blood cells, titers of specific antibody, and lysozyme activities of experimental eels were detected. On 28d post-vaccination, eels from the three groups were challenged by intraperitoneal injection with five different live strains of A. hydrophila (B10, B11, B15, B19, and B20). The results showed that the proliferation of whole blood cells in the OMP group was significantly enhanced on 14d and the serum antibody titers of vaccinated European eels in FKC and OMP group were significantly increased on 28d and 42d. Lysozyme activities in serum were significantly up-regulated in FKC and OMP groups on 21d. The relative percent survival (RPS) of OMP group challenged by A. hydrophila B10, B11, and B20 was 75%, 62.5%, and 88%. This was higher than the corresponding RPS of FKC group with 50%, 37.5%, and 66%, respectively. The RPS was up to 100% in both OMP and FKC group when challenged by A. hydrophila B15 and B19. These results indicate that the 46 kD maltoporin is an effective potent vaccine candidate against different serotypes of A. hydrophila.
[Mh] Termos MeSH primário: Aeromonas hydrophila/imunologia
Anguilla
Antígenos de Bactérias/imunologia
Proteínas da Membrana Bacteriana Externa/imunologia
Vacinas Bacterianas/imunologia
Doenças dos Peixes/prevenção & controle
Infecções por Bactérias Gram-Negativas/veterinária
Porinas/imunologia
Receptores Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos de Bactérias/administração & dosagem
Proteínas da Membrana Bacteriana Externa/genética
Vacinas Bacterianas/administração & dosagem
Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/prevenção & controle
Porinas/genética
Receptores Virais/genética
Vacinação/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Vaccines); 0 (Porins); 0 (Receptors, Virus); 0 (maltoporins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE



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