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[PMID]: | 28829025 |
[Au] Autor: | Blackburn MB; Farrar RR; Sparks ME; Kuhar D; Mitchell A; Gundersen-Rindal DE |
[Ad] Endereço: | Invasive Insect Biocontrol and Behavior Laboratory, USDA, ARS, Bldg 007, Rm 301, BARC-West, Beltsville, MD 20705, USA. |
[Ti] Título: | Chromobacterium sphagni sp. nov., an insecticidal bacterium isolated from Sphagnum bogs. |
[So] Source: | Int J Syst Evol Microbiol;67(9):3417-3422, 2017 Sep. | [Is] ISSN: | 1466-5034 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | Sixteen isolates of Gram-reaction-negative, motile, violet-pigmented bacteria were isolated from Sphagnum bogs in West Virginia and Maine, USA. 16S rRNA gene sequences and fatty acid analysis revealed a high degree of relatedness among the isolates, and genome sequencing of two isolates, IIBBL 14B-1T and IIBBL 37-2 (from West Virginia and Maine, respectively), revealed highly similar genomic sequences. The average nucleotide identity (gANI) calculated for these two isolates was found to be in excess of 99 %, but did not exceed 88 % when comparing either isolate with genomic sequences of Chromobacterium violaceum ATCC 12472T, C. haemolyticum DSM 19808T, C. piscinae ND17, C. subtsugae PRAA4-1T, C. vaccinii MWU205T or C. amazonense CBMAI 310T. Collectively, gANI and 16S rRNA gene sequence comparisons suggested that isolates IIBBL 14B-1T and IIBBL 37-2 were most closely related to C. subtsugae, but represented a distinct species. We propose the name Chromobacterium sphagni sp. nov. for this taxon; the type strain is IIBBL 14B-1T (=NRRL B-67130T=JCM 31882T). |
[Mh] Termos MeSH primário: |
Chromobacterium/classificação Filogenia Sphagnopsida/microbiologia Zonas Úmidas
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[Mh] Termos MeSH secundário: |
Técnicas de Tipagem Bacteriana Composição de Bases Chromobacterium/genética Chromobacterium/isolamento & purificação DNA Bacteriano/genética Maine RNA Ribossômico 16S/genética Análise de Sequência de DNA West Virginia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 170920 |
[Lr] Data última revisão:
| 170920 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170823 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1099/ijsem.0.002127 |
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