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[PMID]:29446279
[Au] Autor:Anganova EV; Savchenkov MF; Stepanenko LA; Savilov ED
[Ti] Título:[Microbiological monitoring of opportunistic Enterobacteriaceae of the Lena river].
[So] Source:Gig Sanit;95(12):1124-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The aim is the study of the circulation, biological activity, persistent potential and genotypic characteristics of opportunistic Enterobacteriaceae of microbial community of the Lena River which is the one of the largest sources of water in Russia and Siberia. Gram-negative component of microbial community in the Lena River was mainly represented by Enterobacteriaceae (80%), including dominated Escherichia (dominant class) and Enterobacter and Klebsiella (subdominant class). In conditions of anthropogenic pollution there is a reorganization of microbial community of the Lena River in the direction of the increase in the proportion of opportunistic pathogens, their species diversity; there is a change of the biological activity of microorganisms, gains of share of strains characterized by antilysozyme activity, hemolytic activity, production DNase, phosphatase. The parameters of system "lysozyme-antilysozyme" are changing, the frequency of the occurrence of bacteria with antilysozyme activity is increasing. This shows the restructurization of the microbial community of water objects. Microorganisms of water objects in territories of anthropogenic pollution are characterized by multiple antibiotic resistance. The proportion of gram-negative opportunistic Enterobacteriaceae in microbial communities of water ecosystems determines the potential danger of water objects and the impact on the level of antibiotic resistance of bacteria. Opportunistic Enterobacteriaceae of microbial community of the Lena River near the city of Yakutsk are characterized by the presence of genetic determinants of pathogenicity (hlyA and sfaG). This is indicative of their potential epidemiological relevance. Microorganisms with high biological activity are markers of their epidemiological danger.
[Mh] Termos MeSH primário: Infecções por Enterobacteriaceae
Enterobacteriaceae
Consórcios Microbianos/fisiologia
Rios/microbiologia
Microbiologia da Água/normas
[Mh] Termos MeSH secundário: Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/patogenicidade
Enterobacteriaceae/fisiologia
Infecções por Enterobacteriaceae/epidemiologia
Infecções por Enterobacteriaceae/prevenção & controle
Monitoramento Ambiental/métodos
Monitoramento Ambiental/estatística & dados numéricos
Seres Humanos
Sibéria/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE


  2 / 16560 MEDLINE  
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[PMID]:29424503
[Au] Autor:Rakhmanin YA; Ivanova LV; Artemova TZ; Gipp EK; Zagaynova AV; Maksimkina TN; Krasnyak AV; Zhuravlev PV; Aleshnya VV; Panasovets OP
[Ti] Título:[Comparative assessment of sanitary and epidemic importance of indicator coliform indices of drinking water quality].
[So] Source:Gig Sanit;95(6):582-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The used methodology of the scientific substantiation of indicators is in the establishment of the conformity of laws of vital activity of indicator and pathogenic microorganisms in the real conditions of the action of the complex of factors, including disinfecting agents. In the one water sample simultaneously there were determined both the general indicator (GICB), thermotolerant (TTCB), glucose positive (GPCB) coliform bacteria, E.coli. On the base of long-term research in the various regions of the Russian Federation, as well with bearing in mind the analysis of domestic and foreign data, comparing the water quality and the incidence of intestinal infections in population it is recommended to use the index of determination of the total number glucose positive coliform bacteria (GPCB), which brings together a much broader range of bacteria of the Enterobacteriaceae family in comparison with total coliform bacteria (TCB) and thermotolerant coliform bacteria (TTCB) and warrants the absence in the test volume of water as an indicator lactose positive (E.coli, TCB, TTCB) and pathogens (salmonella) and potentially pathogenic bacteria which do not ferment lactose. Proposed index of GPCB is shown to allow to assess epidemiological risks not only more accurate, but also more efficiently without increasing the cost performance of bacteriological research.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Água Potável
Infecções por Enterobacteriaceae
Enterobacteriaceae/isolamento & purificação
Microbiologia da Água/normas
[Mh] Termos MeSH secundário: Água Potável/análise
Água Potável/microbiologia
Água Potável/normas
Infecções por Enterobacteriaceae/epidemiologia
Infecções por Enterobacteriaceae/etiologia
Infecções por Enterobacteriaceae/prevenção & controle
Monitoramento Ambiental/métodos
Seres Humanos
Federação Russa/epidemiologia
Qualidade da Água/normas
Abastecimento de Água/métodos
Abastecimento de Água/normas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


  3 / 16560 MEDLINE  
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[PMID]:29390396
[Au] Autor:Eguchi H; Hotta F; Kuwahara T; Nakayama-Imaohji H; Kusaka S; Shimomura Y
[Ad] Endereço:Department of Ophthalmology, Kindai University Sakai Hospital, Harayamadai, Minami-ku, Sakai, Osaka, Japan.
[Ti] Título:Acute keratoconjunctivitis due to contamination of contact lens care solution with histamine-producing Raoultella species: A case report.
[So] Source:Medicine (Baltimore);96(50):e9310, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Contact lens storage cases are known to be contaminated by a significant number of bacteria. However, histamine-producing Raoultella species has not been reported to contaminate contact lens storage case. PATIENT CONCERNS: A 27-year-old woman with keratoconjunctivitis that developed in the left eye owing to a cosmetic contact lens and poor hygiene was referred to our hospital. The corrected visual acuity was hand motion. DIAGNOSES: Corneal infection other than Acanthamoeba keratitis (AK) and corneal hypoxia were excluded. INTERVENTIONS: We initiated empirical therapy for AK, although no cysts or trophozoites were detected in the cornea and in the lens care solution. Analysis of 16S rDNA sequences from the lens care solution yielded the highest homology with Raoultella species, which are histamine-producing bacteria. Histamine was estimated to be 492 ng/mL in the lens care solution. OUTCOMES: Her clinical course was distinct from that of usual AK cases. The corrected visual acuity increased up to (1.2) only 5 days after initiating empirical therapy. LESSONS: To our knowledge, this is the first report to indicate an association between histamine-producing bacteria and keratoconjunctivitis. We should pay an attention to the microbial contamination of contact lens storage cases by histamine producing bacteria.
[Mh] Termos MeSH primário: Soluções para Lentes de Contato
Contaminação de Medicamentos
Infecções por Enterobacteriaceae/microbiologia
Enterobacteriaceae/isolamento & purificação
Ceratoconjuntivite/microbiologia
[Mh] Termos MeSH secundário: Adulto
Diagnóstico Diferencial
Feminino
Seres Humanos
Acuidade Visual
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contact Lens Solutions)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009310


  4 / 16560 MEDLINE  
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[PMID]:29311457
[Au] Autor:Kenzaka T; Kataoka K; Fujimitsu T; Tani K
[Ad] Endereço:Environmental Science and Microbiology, Faculty of Pharmacy, Osaka Ohtani University.
[Ti] Título:[Intestinal Microbiota in Migrating Barn Swallows around Osaka].
[So] Source:Yakugaku Zasshi;138(1):117-122, 2018.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Migratory birds are considered as vectors of infectious diseases, owing to their potential for transmitting pathogens over large distances. The populations of barn swallow (Hirundo rustica) migrate from Southeast Asia to the Japanese mainland during spring and migrate back to Southeast Asia during autumn. This migratory population is estimated to comprise approximately hundreds to thousands of individuals per year. However, to date, not much is known about the gastrointestinal microbiota of the barn swallow. In this study, we characterized the fecal bacterial community in barn swallow. Using 16S rRNA gene metagenomic sequencing analysis, we examined the presence and composition of potentially pathogenic bacteria in the fecal samples, which were collected during spring season from Osaka. The number (±S.D.) of total bacteria was approximately 2.1(±3.4)×10 per gram of feces. In most samples, the bacterial community composition was dominated by families, such as Enterobacteriaceae, Pseudomonadaceae, Mycoplasmataceae, Enterococcaceae, Streptococcaceae, and Alcaligenaceae. However, no relationship was found between the bacterial community composition and geographical area in the fecal samples. Potentially pathogenic bacteria were detected at the rate of >0.1%, which included Pseudomonas spp., Escherichia/Shigella spp., Enterobacter spp., Yersinia spp., Mycoplasma spp., Enterococcus spp., Achromobacter spp., and Serratia spp. Our results suggested that barn swallow is instrumental in the transmission of these genera over large distances.
[Mh] Termos MeSH primário: Migração Animal/fisiologia
Vetores de Doenças
Intestinos/microbiologia
Microbiota
Andorinhas/microbiologia
[Mh] Termos MeSH secundário: Alcaligenaceae/isolamento & purificação
Alcaligenaceae/patogenicidade
Animais
Ásia Sudeste
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/patogenicidade
Enterococcaceae/isolamento & purificação
Enterococcaceae/patogenicidade
Fezes/microbiologia
Japão
Mycoplasmataceae/isolamento & purificação
Mycoplasmataceae/patogenicidade
Pseudomonadaceae/isolamento & purificação
Pseudomonadaceae/patogenicidade
Streptococcaceae/isolamento & purificação
Streptococcaceae/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.17-00148


  5 / 16560 MEDLINE  
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[PMID]:28449738
[Au] Autor:Mahon BM; Brehony C; McGrath E; Killeen J; Cormican M; Hickey P; Keane S; Hanahoe B; Dolan A; Morris D
[Ad] Endereço:Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway, Ireland.
[Ti] Título:Indistinguishable NDM-producing Escherichia coli isolated from recreational waters, sewage, and a clinical specimen in Ireland, 2016 to 2017.
[So] Source:Euro Surveill;22(15), 2017 Apr 13.
[Is] ISSN:1560-7917
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:In this study, New Delhi metallo-beta-lactamase (NDM)-producing Enterobacteriaceae were identified in Irish recreational waters and sewage. Indistinguishable NDM-producing Escherichia coli by pulsed-field gel electrophoresis were isolated from sewage, a fresh water stream and a human source. NDM-producing Klebsiella pneumoniae isolated from sewage and seawater in the same area were closely related to each other and to a human isolate. This raises concerns regarding the potential for sewage discharges to contribute to the spread of carbapenemase-producing Enterobacteriaceae.
[Mh] Termos MeSH primário: Praias
Enterobacteriaceae/enzimologia
Enterobacteriaceae/isolamento & purificação
Esgotos/microbiologia
Microbiologia da Água
Poluentes da Água/isolamento & purificação
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Enterobacteriaceae/classificação
Fezes/microbiologia
Seres Humanos
Irlanda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sewage); 0 (Water Pollutants); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase NDM-1)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 16560 MEDLINE  
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[PMID]:29257642
[Au] Autor:Shiji M; Sabitha AR; Prabhakar K; Harikumar PS
[Ti] Título:Water quality assessment of Kavvayi Lake of northern Kerala, India using CCME water quality index and biological water quality criteria.
[So] Source:J Environ Biol;37(6):1265-72, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Assessment of water quality status of 7 sites of Kavvayi Wetland in northern Kerala (India) was carried out. The physico-chemical, bacteriological and biological parameters were monitored during pre-monsoon, monsoon and post-monsoon seasons. Canadian Council of Ministers of the Environment (CCME) water quality index of the Kavvayi Lake samples ranged from 43.99-44.77; indicating that water quality was threatened or impaired. The poor water quality status might be due to dumping of wastes from municipal and domestic sources and agricultural runoff. Biological water quality criteria (BWQC) determined for wetland revealed that stations such as mixing point of Kariangode River into Kavvayi Lake and Kottikkadavu was moderately polluted in pre-monsoon and post- monsoon seasons. Mixing point of Nileswar River into Kavvayi Lake was moderately polluted in pre-monsoon season. Both calculated indices suggest that quality of lake was found to be influenced by anthropogenic activities such as unscientific tourism and infrastructure development, land encroachment, sand mining, pollution etc. The study was carried out as part of a programme, which aimed to conserve Kavvayi wetland because of its unique ecological and environmental characteristics.
[Mh] Termos MeSH primário: Monitoramento Ambiental/métodos
Lagos/química
Poluentes Químicos da Água/química
Qualidade da Água
Água/normas
[Mh] Termos MeSH secundário: Enterobacteriaceae/isolamento & purificação
Índia
Microbiologia da Água
Poluição da Água
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 059QF0KO0R (Water)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  7 / 16560 MEDLINE  
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[PMID]:29293526
[Au] Autor:Marcoccia F; Leiros HS; Aschi M; Amicosante G; Perilli M
[Ad] Endereço:Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
[Ti] Título:Exploring the role of L209 residue in the active site of NDM-1 a metallo-ß-lactamase.
[So] Source:PLoS One;13(1):e0189686, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: New Delhi Metallo-ß-Lactamase (NDM-1) is one of the most recent additions to the ß-lactamases family. Since its discovery in 2009, NDM-1 producing Enterobacteriaceae have disseminated globally. With few effective antibiotics against NDM-1 producers, there is an urgent need to design new drug inhibitors through the help of structural and mechanistic information available from mutagenic studies. RESULTS/CONCLUSIONS: In our study we focus the attention on the non-catalytic residue Leucine 209 by changing it into a Phenylalanine. The L209F laboratory variant of NDM-1 displays a drastic reduction of catalytic efficiency (due to low kcat values) towards penicillins, cephalosporins and carbapenems. Thermofluor-based assay demonstrated that NDM-1 and L209F are stable to the temperature and the zinc content is the same in both enzymes as demonstrated by experiments with PAR in the presence of GdnHCL. Molecular Dynamics (MDs) simulations, carried out on NDM-1 and L209F both complexed and uncomplexed with Benzylpenicillin indicate that the point mutation produces a significant mechanical destabilization of the enzyme and also an increase of water content. These observations clearly show that the single mutation induces drastic changes in the enzyme properties which can be related to the observed different catalytic behavior.
[Mh] Termos MeSH primário: beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Domínio Catalítico
Enterobacteriaceae/genética
Cinética
Testes de Sensibilidade Microbiana
Simulação de Dinâmica Molecular
Mutagênese Sítio-Dirigida
beta-Lactamases/química
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase NDM-1)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189686


  8 / 16560 MEDLINE  
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[PMID]:29370234
[Au] Autor:Madhi F; Jung C; Timsit S; Levy C; Biscardi S; Lorrot M; Grimprel E; Hees L; Craiu I; Galerne A; Dubos F; Cixous E; Hentgen V; Béchet S; Bonacorsi S; Cohen R; Urinary-tract Infection due to Extended-Spectrum Beta-lactamase­producing Enterobacteriaceae in Children Group
[Ad] Endereço:Service de Pédiatrie Générale, Centre Hospitalier Intercommunal de Créteil, Créteil, France.
[Ti] Título:Febrile urinary-tract infection due to extended-spectrum beta-lactamase-producing Enterobacteriaceae in children: A French prospective multicenter study.
[So] Source:PLoS One;13(1):e0190910, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To assess the management of febrile urinary-tract infection (FUTIs) due to extended-spectrum ß-lactamase-producing Enterobacteriaceae (ESBL-E) in children, the Pediatric Infectious Diseases Group of the French Pediatric Society set up an active surveillance network in pediatric centers across France in 2014. MATERIALS AND METHODS: We prospectively analysed data from 2014 to 2016 for all children < 18 years old who received antibiotic treatment for FUTI due to ESBL-E in 24 pediatric centers. Baseline demographic, clinical features, microbiological data and antimicrobials prescribed were collected. RESULTS: 301 children were enrolled in this study. The median age was 1 year (IQR 0.02-17.9) and 44.5% were male. These infections occurred in children with history of UTIs (27.3%) and urinary malformations (32.6%). Recent antibiotic use was the main associated factor for FUTIs due to ESBL-E, followed by a previous hospitalization and travel history. Before drug susceptibility testing (DST), third-generation cephalosporins (3GC) PO/IV were the most-prescribed antibiotics (75.5%). Only 13% and 24% of children received amikacine alone for empirical or definitive therapy, respectively, whereas 88.7% of children had isolates susceptible to amikacin. In all, 23.2% of children received carbapenems in empirical and/or definitive therapy. Cotrimoxazole (24.5%), ciprofloxacin (15.6%) and non-orthodox clavulanate-cefixime combination (31.3%) were the most frequently prescribed oral options after obtaining the DST. The time to apyrexia and length of hospital stay did not differ with or without effective empirical therapy. CONCLUSIONS: We believe that amikacin should increasingly take on a key role in the choice of definitive therapy of FUTI due to ESBL-E in children by avoiding the use of carbapenems.
[Mh] Termos MeSH primário: Infecções por Enterobacteriaceae/microbiologia
Enterobacteriaceae/enzimologia
Infecções Urinárias/microbiologia
beta-Lactamases/biossíntese
[Mh] Termos MeSH secundário: Adolescente
Amicacina/uso terapêutico
Antibacterianos/efeitos adversos
Antibacterianos/uso terapêutico
Carbapenêmicos/efeitos adversos
Criança
Pré-Escolar
Enterobacteriaceae/efeitos dos fármacos
Infecções por Enterobacteriaceae/tratamento farmacológico
Infecções por Enterobacteriaceae/epidemiologia
Feminino
Febre/tratamento farmacológico
Febre/microbiologia
França/epidemiologia
Seres Humanos
Lactente
Recém-Nascido
Masculino
Testes de Sensibilidade Microbiana
Estudos Prospectivos
Fatores de Risco
Infecções Urinárias/tratamento farmacológico
Infecções Urinárias/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems); 84319SGC3C (Amikacin); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190910


  9 / 16560 MEDLINE  
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[PMID]:29174735
[Au] Autor:Shalom O; Adler A
[Ad] Endereço:Clinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Israel.
[Ti] Título:Comparative study of 3 carbapenem-hydrolysis methods for the confirmation of carbapenemase production in Enterobacteriaceae.
[So] Source:Diagn Microbiol Infect Dis;90(2):73-76, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The goals of the study were to examine the analytic and performance parameters of 2 commercial rapid carbapenem-hydrolysis assays, the ß-CARBA test (Bio-Rad) and the Rapid CARB Blue Kit (ROSCO) in comparison with an in-house CARBA NP assay for the detection of Carbapenemase-Producing Enterobacteriaceae (CPE). Their performance was evaluated following growth on 2 chromogenic CPE screening media. The sensitivity was highest (91%) in the ß-CARBA test when used from the mSuperCARBA™ plates and was lowest when the same test was used from the chromID™ CARBA plates (75%). The specificity was highest in the NP CARBA test in both media (96%), followed by the ß CARBA/mSuperCARBA™ combination (92%). The specificity of the Rapid CARB Blue Kit was as low as 36% when used with the chromID™ CARBA plates. The ß-CARBA test was simple to use and had the shortest turn-around time and hand-on time.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Proteínas de Bactérias/metabolismo
Técnicas de Tipagem Bacteriana/métodos
Carbapenêmicos/metabolismo
Enterobacteriaceae/enzimologia
Resistência beta-Lactâmica
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/análise
Técnicas de Tipagem Bacteriana/normas
Enterobacteriaceae/metabolismo
Infecções por Enterobacteriaceae/microbiologia
Seres Humanos
Hidrólise
Sensibilidade e Especificidade
beta-Lactamases/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carbapenems); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  10 / 16560 MEDLINE  
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[PMID]:29388538
[Au] Autor:Vanstone GL; Woodhead S; Roulston K; Sharma H; Wey E; Smith ER; Mack D; Balakrishnan I
[Ad] Endereço:1​HSL Analytics LLP, 1 Mabledon Place, London WC1H 9AJ, UK.
[Ti] Título:Improving the detection of carbapenemase-producing organisms (CPO) in a low-prevalence setting: evaluation of four commercial methods and implementation of an algorithm of testing.
[So] Source:J Med Microbiol;67(2):208-214, 2018 Feb.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Carbapenemase-producing organisms (CPOs) can be resistant to almost all ß-lactams and represent an increasing threat in healthcare facilities. Detection of these organisms in routine diagnostic laboratories is difficult; here we evaluate four commercially available CPO detection assays and assess their suitability for the clinical laboratory. METHODOLOGY: A panel of 95 clinical multidrug-resistant organisms (22 NDM, 24 OXA-48, 19 VIM, 4 OXA-23, 3 KPC, 4 NDM+OXA-48, 1 OXA23+NDM, 1 IMI, 1 IMP-1, 9 ESBL, 3 derepressed AmpC and 4 inducible AmpC producers) were tested by the RESIST-3 O.K.N., RapidEC CarbaNP, Acuitas Resistome and Xpert Carba-R assays.Results/Key Findings. The commercial assays performed well, with high sensitivities (96.2-100 %) and specificities (all, 100 %). The RapidEC CarbaNP and Acuitas Resistome were able to detect the broadest range of carbapenemase genotypes. The RESIST-3 O.K.N. and Xpert CarbaR had the shortest turnaround times, whilst the RapidEC CarbaNP was the only assay included in this study that could detect previously undescribed genotypes. CONCLUSION: Using an algorithm of the RapidEC CarbaNP, followed by either the RESIST-3 O.K.N. (Enterobacteriaceae) or the Xpert Carba-R (Pseudomonas aeruginosa and Acinetobacter spp.) on suspect CPOs allowed rapid in-house detection and genotyping of a high proportion of CPOs, reducing turnaround time by up to 7 days.
[Mh] Termos MeSH primário: Algoritmos
Proteínas de Bactérias/biossíntese
Técnicas Bacteriológicas
Bactérias Gram-Negativas/enzimologia
Bactérias Gram-Negativas/isolamento & purificação
Kit de Reagentes para Diagnóstico/normas
beta-Lactamases/biossíntese
[Mh] Termos MeSH secundário: Acinetobacter/efeitos dos fármacos
Acinetobacter/enzimologia
Acinetobacter/genética
Acinetobacter/isolamento & purificação
Proteínas de Bactérias/análise
Proteínas de Bactérias/genética
Farmacorresistência Bacteriana Múltipla
Enterobacteriaceae/efeitos dos fármacos
Enterobacteriaceae/enzimologia
Enterobacteriaceae/genética
Enterobacteriaceae/isolamento & purificação
Infecções por Enterobacteriaceae/diagnóstico
Infecções por Enterobacteriaceae/microbiologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/enzimologia
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Genótipo
Bactérias Gram-Negativas/genética
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
Pseudomonas aeruginosa/isolamento & purificação
Sensibilidade e Especificidade
beta-Lactamases/análise
beta-Lactamases/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Reagent Kits, Diagnostic); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000674



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