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  1 / 1114 MEDLINE  
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[PMID]:28857032
[Au] Autor:Ribeiro TG; Gonçalves BR; da Silva MS; Novais Â; Machado E; Carriço JA; Peixe L
[Ad] Endereço:1​UCIBIO-REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Portugal.
[Ti] Título:Citrobacter portucalensis sp. nov., isolated from an aquatic sample.
[So] Source:Int J Syst Evol Microbiol;67(9):3513-3517, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative strain, A60T, isolated from a water well sample in Portugal, was characterized phenotypically, genotypically and phylogenetically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A60T belonged to the genus Citrobacter, and recN gene phylogeny revealed one strongly supported clade encompassing strain A60T and 13 other strains from public databases, distinct from currently recognized species of the genus Citrobacter. Furthermore, multilocus sequence analysis (MLSA) based on concatenated partial fusA, leuS, pyrG and rpoB sequences confirmed the classification obtained with the recN sequence. In silico genomic comparisons, including average nucleotide identity (ANI) and the genome-to-genome distance calculator (GGDC), showed 94.6 % and 58.4 % identity to the closest relative Citrobacter freundii ATCC 8090T, respectively. The ability to metabolize different compounds further discriminated strain A60T from other species of the genus Citrobacter. The G+C content of strain A60T is 52.0 %. The results obtained support the description of a novel species within the genus Citrobacter, for which the name Citrobacter portucalensis sp. nov. is proposed, with the type strain A60T (=DSM 104542T=CECT 9236T).
[Mh] Termos MeSH primário: Citrobacter/classificação
Filogenia
Microbiologia da Água
Poços de Água
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Citrobacter/genética
Citrobacter/isolamento & purificação
DNA Bacteriano/genética
Genes Bacterianos
Tipagem de Sequências Multilocus
Portugal
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002154


  2 / 1114 MEDLINE  
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[PMID]:28559163
[Au] Autor:Sánchez-Calvo JM; de Francisco JL; Torres-Martos E; Alados Arboledas JC; López Prieto MD
[Ad] Endereço:AGS Norte de Cádiz, Hospital SAS de Jerez, Jerez de la Frontera, Cádiz, Spain. Electronic address: jmanuel.sanchez.sspa@juntadeandalucia.es.
[Ti] Título:A cost-saving strategy for processing isolated uropathogens in community-acquired urinary tract infections.
[So] Source:J Microbiol Methods;139:130-134, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total). In the second period, a total 11,728 bacterial isolates were processed, with annual estimated costs of EUR 21,078 and EUR 23,248, respectively (EUR 44,326 total). We included the cost for a laboratory technician (252h worked per year), estimated at EUR 2500 per year. The mean estimated savings were EUR 66,797 (60%).The identification by chromogenic media and antibiotic susceptibility patterns by disk diffusion method was similar to MicroScan in both study periods. Only some isolated Citrobacter spp., Enterobacter spp., Morganella morganii, and Providencia spp. were misidentified. The strategy reported here did not affect the quality of the results and yielded substantial cost savings.
[Mh] Termos MeSH primário: Bactérias/efeitos dos fármacos
Bactérias/isolamento & purificação
Infecções Comunitárias Adquiridas/microbiologia
Testes de Sensibilidade Microbiana/economia
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Compostos Cromogênicos/química
Compostos Cromogênicos/economia
Citrobacter/efeitos dos fármacos
Citrobacter/isolamento & purificação
Citrobacter/patogenicidade
Redução de Custos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/economia
Farmacorresistência Bacteriana
Enterobacter/efeitos dos fármacos
Enterobacter/isolamento & purificação
Enterobacter/patogenicidade
Seres Humanos
Testes de Sensibilidade Microbiana/métodos
Urina/microbiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Chromogenic Compounds)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE


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[PMID]:28376885
[Au] Autor:Yumoto T; Kono Y; Kawano S; Kamoi C; Iida A; Nose M; Sato K; Ugawa T; Okada H; Ujike Y; Nakao A
[Ad] Endereço:Advanced Emergency and Critical Care Medical Center, Okayama University Hospital, 2-5-1 Kita-ku, Shikata-cho, Okayama-shi, Okayama, 700-8558, Japan. tyumoto@cc.okayama-u.ac.jp.
[Ti] Título:Citrobacter braakii bacteremia-induced septic shock after colonoscopy preparation with polyethylene glycol in a critically ill patient: a case report.
[So] Source:Ann Clin Microbiol Antimicrob;16(1):22, 2017 Apr 04.
[Is] ISSN:1476-0711
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Polyethylene glycol (PEG) is widely used for bowel cleaning in preparation for colonoscopy because of its safety. Septic shock after PEG preparation is an extremely rare complication. Herein, we describe a case of septic shock that occurred immediately after colonoscopy preparation with PEG. CASE PRESENTATION: A 75-year-old Japanese male who had previously developed diabetes after total pancreatectomy received PEG in preparation for colonoscopy. He had been admitted to the emergency intensive care unit 4 days earlier due to hematochezia presenting with shock. He ingested PEG to prepare for a colonoscopy examination, which was performed to identify the source of his bleeding over a 5-h period, but suddenly exhibited septic shock and markedly elevated procalcitonin levels. A blood culture subsequently revealed Citrobacter braakii. Immediate resuscitation and intensive care with appropriate antibiotics improved his condition. CONCLUSIONS: Clinicians should be aware of the possibility of deteriorating conditions after bowel preparation with PEG among severely ill patients with recent episodes of hemorrhagic shock.
[Mh] Termos MeSH primário: Citrobacter/isolamento & purificação
Infecções por Enterobacteriaceae/induzido quimicamente
Infecções por Enterobacteriaceae/complicações
Polietilenoglicóis/efeitos adversos
Choque Séptico/etiologia
Choque Séptico/patologia
[Mh] Termos MeSH secundário: Idoso
Grupo com Ancestrais do Continente Asiático
Sangue/microbiologia
Estado Terminal
Seres Humanos
Masculino
Polietilenoglicóis/administração & dosagem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
30IQX730WE (Polyethylene Glycols)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1186/s12941-017-0201-5


  4 / 1114 MEDLINE  
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[PMID]:28364327
[Au] Autor:Owoseni M; Okoh A
[Ad] Endereço:SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, 5700, South Africa. moji.owoseni@gmail.com.
[Ti] Título:Assessment of chlorine tolerance profile of Citrobacter species recovered from wastewater treatment plants in Eastern Cape, South Africa.
[So] Source:Environ Monit Assess;189(4):201, 2017 Apr.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This present study assessed the chlorine tolerance of some Citrobacter species recovered from secondary effluents from the clarifiers of two wastewater treatment plants in the Eastern Cape, South Africa. The bacterial survival, chlorine lethal dose and inactivation kinetics at lethal doses were examined. Inactivation of the test bacteria (n = 20) at the recommended dose of 0.5 mg/l for 30 min exposure showed a progressive reduction in bacterial population from 4 to 5 log reduction and residuals ranged between 0.12 and 0.46 mg/l. The bactericidal activity of chlorine increased at higher dosages with a substantial reduction in viability of the bacteria and complete inactivation of the bacterial population at a lethal dose of 0.75 and 1.0 mg/l in 30 min. For the inactivation kinetics, bactericidal activity of chlorine increased with time showing a 3.67-5.4 log reduction in 10 min, 4.0-5.6 log reduction in 20 min and above 6.3 log reductions to complete sterilization of bacterial population over 30 min for all the entire test Citrobacter isolates used in this study. Furthermore, there was a strong correlation (R  > 0.84) between bacteria inactivation and increase in contact time. This study appears to have provided support for laboratory evidence of bacterial tolerance to chlorine disinfection at current recommended dose (0.5 mg/l for 30 min), and chlorine concentration between 0.75 and 1.0 mg/l was found to have a better disinfecting capacity to check tolerance of Citrobacter species.
[Mh] Termos MeSH primário: Cloro/farmacologia
Citrobacter/fisiologia
Desinfetantes/farmacologia
Viabilidade Microbiana
Águas Residuais/análise
[Mh] Termos MeSH secundário: Desinfecção
Monitoramento Ambiental
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Waste Water); 4R7X1O2820 (Chlorine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170402
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-017-5900-z


  5 / 1114 MEDLINE  
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[PMID]:28276281
[Au] Autor:Maraki S; Vardakas KZ; Mavromanolaki VE; Kyriakidou M; Spais G; Kofteridis DP; Samonis G; Falagas ME
[Ad] Endereço:a Department of Clinical Microbiology , University Hospital of Heraklion , Heraklion , Greece.
[Ti] Título:In vitro susceptibility and resistance phenotypes in contemporary Citrobacter isolates in a University Hospital in Crete, Greece.
[So] Source:Infect Dis (Lond);49(7):532-539, 2017 Jul.
[Is] ISSN:2374-4243
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Data on Citrobacter spp. susceptibility are scarce. We sought to study the evolution in the susceptibility of 385 Citrobacter spp. at the University Hospital of Heraklion, Crete, Greece during a six-year period (2010-2015). METHODS: Non-duplicate strains isolated from inpatients (intensive care unit, oncology, surgery, internal medicine, paediatrics) and outpatients were studied using Vitek 2. Phenotypic confirmatory tests were applied for detection of ß-lactamases and aminoglycoside modifying enzymes. RESULTS: C. freundii (172, 44.7%) and C. koseri (166, 43.1%) were the most commonly isolated species. C. braakii (34), C. amalonaticus (6), C. youngae (6) and C. sedlakii (1) were the remaining isolates. Colistin and fosfomycin were the most active antibiotics (both 99.2%) followed by carbapenems (99%) aminoglycosides (96.6-98.4%), tigecycline (96.1%), cefepime (94.8%), ciprofloxacin (94.3%), tetracycline (92.7%), trimethoprim/sulphamethoxazole (91.4%), chloramphenicol (88.1%), piperacillin/tazobactam (86.5%) and 3rd generation cephalosporins (85.7%). C. freundii were more resistant than C. koseri. Antibiotic resistance did not increase during the study period for most antibiotics. Lower susceptibility to all antibiotics was observed among multi-drug resistant (MDR) strains. AmpC was the most common resistant mechanism (10.9%); carbapenemases (1.3%) and aminoglycoside modifying enzymes (2.9%) were also detected. All AmpC producers were resistant to cephalosporins but not to carbapenems. In all but one isolates aminoglycoside resistance was accompanied by acquired ß-lactamases. CONCLUSIONS: Although Citrobacter species in general were susceptible, antibiotic susceptibility testing is required for the detection of resistant isolates.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Citrobacter/efeitos dos fármacos
Citrobacter/isolamento & purificação
Farmacorresistência Bacteriana
Infecções por Enterobacteriaceae/microbiologia
[Mh] Termos MeSH secundário: Citrobacter/classificação
Infecções por Enterobacteriaceae/epidemiologia
Grécia/epidemiologia
Hospitais Universitários
Seres Humanos
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/23744235.2017.1297896


  6 / 1114 MEDLINE  
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[PMID]:28257478
[Au] Autor:Almand EA; Moore MD; Outlaw J; Jaykus LA
[Ad] Endereço:Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States of America.
[Ti] Título:Human norovirus binding to select bacteria representative of the human gut microbiota.
[So] Source:PLoS One;12(3):e0173124, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. The purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. The bacteria screened included naturally occurring strains isolated from human stool (Klebsiella spp., Citrobacter spp., Bacillus spp., Enterococcus faecium and Hafnia alvei) and select reference strains (Staphylococcus aureus and Enterobacter cloacae). Binding in PBS was evaluated to three human norovirus strains (GII.4 New Orleans 2009 and Sydney 2012, GI.6) and two surrogate viruses (Tulane virus and Turnip Crinkle Virus (TCV)) using a suspension assay format linked to RT-qPCR for quantification. The impact of different overnight culture media prior to washing on binding efficiency in PBS was also evaluated, and binding was visualized using transmission electron microscopy. All bacteria tested bound the representative human norovirus strains with high efficiency (<1 log10 of input virus remained unbound or <10% unbound and >90% binding efficiency) (p>0.05); there was selective binding for Tulane virus and no binding observed for TCV. Binding efficiency was highest when bacteria were cultured in minimal media (<1 log10 of input virus remained unbound, so >90% bound), but notably decreased when cultured in enriched media (1-3 log10 unbound or 0.01 -<90% bound)) (p<0.05). The norovirus-bacteria binding occurred around the outer cell surfaces and pili structures, without apparent localization. The findings reported here further elucidate and inform the dynamics between human noroviruses and enteric bacteria with implications for norovirus pathogenesis.
[Mh] Termos MeSH primário: Fímbrias Bacterianas/metabolismo
Microbioma Gastrointestinal/fisiologia
Interações Microbianas
Norovirus/metabolismo
[Mh] Termos MeSH secundário: Bacillus/isolamento & purificação
Bacillus/metabolismo
Citrobacter/isolamento & purificação
Citrobacter/metabolismo
Meios de Cultura/química
Enterobacter cloacae/isolamento & purificação
Enterobacter cloacae/metabolismo
Enterococcus faecium/isolamento & purificação
Enterococcus faecium/metabolismo
Fezes/microbiologia
Fímbrias Bacterianas/química
Fímbrias Bacterianas/ultraestrutura
Hafnia alvei/isolamento & purificação
Hafnia alvei/metabolismo
Seres Humanos
Klebsiella/isolamento & purificação
Klebsiella/metabolismo
Norovirus/ultraestrutura
Staphylococcus aureus/isolamento & purificação
Staphylococcus aureus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173124


  7 / 1114 MEDLINE  
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[PMID]:28199403
[Au] Autor:Birnie K; Hay AD; Wootton M; Howe R; MacGowan A; Whiting P; Lawton M; Delaney B; Downing H; Dudley J; Hollingworth W; Lisles C; Little P; O'Brien K; Pickles T; Rumsby K; Thomas-Jones E; Van der Voort J; Waldron CA; Harman K; Hood K; Butler CC; Sterne JA
[Ad] Endereço:School of Social and Community Medicine, University of Bristol, Bristol, United Kingdom.
[Ti] Título:Comparison of microbiological diagnosis of urinary tract infection in young children by routine health service laboratories and a research laboratory: Diagnostic cohort study.
[So] Source:PLoS One;12(2):e0171113, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To compare the validity of diagnosis of urinary tract infection (UTI) through urine culture between samples processed in routine health service laboratories and those processed in a research laboratory. POPULATION AND METHODS: We conducted a prospective diagnostic cohort study in 4808 acutely ill children aged <5 years attending UK primary health care. UTI, defined as pure/predominant growth ≥105 CFU/mL of a uropathogen (the reference standard), was diagnosed at routine health service laboratories and a central research laboratory by culture of urine samples. We calculated areas under the receiver-operator curve (AUC) for UTI predicted by pre-specified symptoms, signs and dipstick test results (the "index test"), separately according to whether samples were obtained by clean catch or nappy (diaper) pads. RESULTS: 251 (5.2%) and 88 (1.8%) children were classified as UTI positive by health service and research laboratories respectively. Agreement between laboratories was moderate (kappa = 0.36; 95% confidence interval [CI] 0.29, 0.43), and better for clean catch (0.54; 0.45, 0.63) than nappy pad samples (0.20; 0.12, 0.28). In clean catch samples, the AUC was lower for health service laboratories (AUC = 0.75; 95% CI 0.69, 0.80) than the research laboratory (0.86; 0.79, 0.92). Values of AUC were lower in nappy pad samples (0.65 [0.61, 0.70] and 0.79 [0.70, 0.88] for health service and research laboratory positivity, respectively) than clean catch samples. CONCLUSIONS: The agreement of microbiological diagnosis of UTI comparing routine health service laboratories with a research laboratory was moderate for clean catch samples and poor for nappy pad samples and reliability is lower for nappy pad than for clean catch samples. Positive results from the research laboratory appear more likely to reflect real UTIs than those from routine health service laboratories, many of which (particularly from nappy pad samples) could be due to contamination. Health service laboratories should consider adopting procedures used in the research laboratory for paediatric urine samples. Primary care clinicians should try to obtain clean catch samples, even in very young children.
[Mh] Termos MeSH primário: Instalações de Saúde
Laboratórios
Infecções Urinárias/diagnóstico
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Área Sob a Curva
Pré-Escolar
Citrobacter/isolamento & purificação
Estudos de Coortes
Testes Diagnósticos de Rotina
Enterobacter/isolamento & purificação
Escherichia coli/isolamento & purificação
Feminino
Seres Humanos
Lactente
Recém-Nascido
Klebsiella/isolamento & purificação
Masculino
Razão de Chances
Estudos Prospectivos
Curva ROC
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171113


  8 / 1114 MEDLINE  
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[PMID]:28121036
[Au] Autor:Scheufele F; Aichinger L; Jäger C; Demir IE; Schorn S; Sargut M; Erkan M; Kleeff J; Friess H; Ceyhan GO
[Ad] Endereço:Department of Surgery, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
[Ti] Título:Effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer.
[So] Source:Br J Surg;104(2):e182-e188, 2017 Jan.
[Is] ISSN:1365-2168
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (PBD). The effect of PBD on the microbiome of the biliary system and on postoperative outcome remains unclear. METHODS: A single-centre retrospective study of patients with obstructive jaundice due to periampullary cancer, treated between July 2007 and July 2015, was undertaken. Intraoperative bile samples were obtained for microbiological analysis after transection of the common bile duct. Postoperative complications were registered. RESULTS: Of 290 patients treated, intraoperative bile samples were present for 172 patients (59·3 per cent) who had PBD and 118 (40·7 per cent) who did not. Contamination of bile was increased significantly in patients who underwent stenting (97·1 per cent versus 18·6 per cent in those without stenting; P < 0·001). PBD resulted in a shift in the biliary microbiome from Escherichia coli in non-stented patients (45 per cent versus 19·2 per cent in stented patients; P = 0·009) towards increased contamination with Enterococcus faecalis (9 versus 37·7 per cent respectively; P = 0·008) and Enterobacter cloacae (0 versus 20·4 per cent; P = 0·033). This shift was associated with a high incidence of bacterial resistance against ampicillin-sulbactam (63·6 per cent versus 18 per cent in patients with no PBD; P < 0·001), piperacillin-tazobactam (30·1 versus 0 per cent respectively; P = 0·003), ciprofloxacin (28·5 versus 5 per cent; P = 0·047) and imipenem (26·6 versus 0 per cent; P = 0·011). The rate of wound infection was higher in patients with a positive bile culture (21·0 per cent versus 6 per cent in patients with sterile bile; P = 0·002). Regression analysis revealed the presence of Enterococcus faecium (odds ratio 2·83, 95 per cent c.i. 1·17 to 6·84; P = 0·021) and Citrobacter species (odds ratio 5·09, 1·65 to 15·71; P = 0·005) as independent risk factors for postoperative wound infection. CONCLUSION: There are fundamental differences in the biliary microbiome of patients with periampullary cancer who undergo PBD and those who do not. PBD induces a shift of the biliary microbiome towards a more aggressive and resistant spectrum, which requires a differentiated perioperative antibiotic treatment strategy.
[Mh] Termos MeSH primário: Bile/microbiologia
Neoplasias do Ducto Colédoco/complicações
Drenagem
Icterícia Obstrutiva/terapia
Microbiota
Cuidados Pré-Operatórios
[Mh] Termos MeSH secundário: Idoso
Ampola Hepatopancreática/cirurgia
Antibacterianos/uso terapêutico
Colangite/epidemiologia
Citrobacter/isolamento & purificação
Neoplasias do Ducto Colédoco/cirurgia
Farmacorresistência Bacteriana Múltipla
Infecções por Enterobacteriaceae/epidemiologia
Enterococcus faecalis/isolamento & purificação
Feminino
Alemanha/epidemiologia
Infecções por Bactérias Gram-Positivas/epidemiologia
Seres Humanos
Icterícia Obstrutiva/etiologia
Masculino
Análise de Regressão
Estudos Retrospectivos
Fatores de Risco
Stents
Infecção da Ferida Cirúrgica/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1002/bjs.10450


  9 / 1114 MEDLINE  
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[PMID]:28040649
[Au] Autor:Takenaka M; Yoon KS; Matsumoto T; Ogo S
[Ad] Endereço:International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
[Ti] Título:Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels.
[So] Source:Bioresour Technol;227:279-285, 2017 Mar.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pyruvate ferredoxin oxidoreductase from Citrobacter sp. S-77 (PFOR ) was purified in order to develop a method for acetyl-CoA production. Although the purified PFOR showed high O -sensitivity, the activity could be remarkably stabilized in anaerobic conditions. PFOR was effectively immobilized on ceramic hydroxyapatite (PFOR -HA) with an efficiency of more than 96%, however, after encapsulation of PFOR -HA in alginate, the rate of catalytic acetyl-CoA production was highly reduced to 36% when compared to that of the free enzyme. However, the operational stability of the PFOR -HA in alginate hydrogels was remarkable, retaining over 68% initial activity even after ten repeated cycles. The results suggested that the PFOR -HA hydrogels have a high potential for biotechnological application.
[Mh] Termos MeSH primário: Acetilcoenzima A/síntese química
Alginatos/química
Hidrogéis/química
Piruvato Sintase/química
[Mh] Termos MeSH secundário: Catálise
Citrobacter/enzimologia
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Piruvato Sintase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Hexuronic Acids); 0 (Hydrogels); 72-89-9 (Acetyl Coenzyme A); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); EC 1.2.7.1 (Pyruvate Synthase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:28034519
[Au] Autor:Moy S; Sharma R
[Ad] Endereço:Department of Pharmacy, State University of New York (SUNY), Downstate Medical Center, Brooklyn, New York. Electronic address: Stanley.Moy@downstate.edu.
[Ti] Título:Treatment Outcomes in Infections Caused by "SPICE" (Serratia, Pseudomonas, Indole-positive Proteus, Citrobacter, and Enterobacter) Organisms: Carbapenem versus Noncarbapenem Regimens.
[So] Source:Clin Ther;39(1):170-176, 2017 Jan.
[Is] ISSN:1879-114X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Techniques used to identify AmpC ß-lactamases in SPICE (Serratia, Pseudomonas, indole-positive Proteus, Citrobacter, and Enterobacter) organisms are not yet optimized for the clinical laboratory and are not routinely used. Clinicians are often left with an uncertainty on the choice of antibiotic when a SPICE organism is isolated. The purpose of this study was to evaluate the outcomes of carbapenem versus noncarbapenem regimens in treating bacteremia or urinary tract infection from a SPICE organism in clinical practice. METHODS: This single-center, retrospective, cohort study analyzed data from adult patients who had clinical infection with a SPICE organism isolated from blood or urine cultures. Patients were assigned to a carbapenem- or noncarbapenem-treated group. The primary end point was clinical response, defined as a resolution of signs and symptoms of infection at the end of therapy. FINDINGS: A total of 332 patients were assessed, and 145 patients met the inclusion criteria for the study. There were 20 patients who received a carbapenem, while 125 received a noncarbapenem regimen. The percentage of patients who were bacteremic was 46.2%. Clinical response overall was achieved in 80% of patients on a carbapenem versus 90.3% of patients on a noncarbapenem regimen (P = 0.24). The rate of microbiologic cure was 90% in patients on a carbapenem versus 91.2% in patients on a noncarbapenem regimen (P = 1). IMPLICATIONS: In this study in patients treated for infection with a SPICE organism in clinical practice, the rates of clinical response did not differ significantly between the carbapenem and noncarbapenem groups. Current CLSI breakpoints set for SPICE organisms may still be reliable and may not require additional testing for AmpC ß-lactamases.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Bacteriemia/tratamento farmacológico
Carbapenêmicos/uso terapêutico
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Proteínas de Bactérias/metabolismo
Citrobacter/isolamento & purificação
Enterobacter/isolamento & purificação
Feminino
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Proteus/isolamento & purificação
Pseudomonas/isolamento & purificação
Estudos Retrospectivos
Serratia/isolamento & purificação
beta-Lactamases/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carbapenems); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE



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