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  1 / 3672 MEDLINE  
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[PMID]:29049419
[Au] Autor:Orellana R; Chaput G; Markillie LM; Mitchell H; Gaffrey M; Orr G; DeAngelis KM
[Ad] Endereço:Centro de Biotecnología Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Valparaíso, Chile.
[Ti] Título:Multi-time series RNA-seq analysis of Enterobacter lignolyticus SCF1 during growth in lignin-amended medium.
[So] Source:PLoS One;12(10):e0186440, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new ways of removing the lignin that protects cellulose for improved conversion of lignin to fuel precursors. Enterobacter lignolyticus SCF1 is a facultative anaerobic Gammaproteobacteria isolated from tropical rain forest soil collected in El Yunque forest, Puerto Rico under anoxic growth conditions with lignin as sole carbon source. Whole transcriptome analysis of SCF1 during E.lignolyticus SCF1 lignin degradation was conducted on cells grown in the presence (0.1%, w/w) and the absence of lignin, where samples were taken at three different times during growth, beginning of exponential phase, mid-exponential phase and beginning of stationary phase. Lignin-amended cultures achieved twice the cell biomass as unamended cultures over three days, and in this time degraded 60% of lignin. Transcripts in early exponential phase reflected this accelerated growth. A complement of laccases, aryl-alcohol dehydrogenases, and peroxidases were most up-regulated in lignin amended conditions in mid-exponential and early stationary phases compared to unamended growth. The association of hydrogen production by way of the formate hydrogenlyase complex with lignin degradation suggests a possible value added to lignin degradation in the future.
[Mh] Termos MeSH primário: Enterobacter/genética
Lignina/metabolismo
RNA Bacteriano/genética
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Meios de Cultura
Enterobacter/crescimento & desenvolvimento
Enterobacter/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (RNA, Bacterial); 9005-53-2 (Lignin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186440


  2 / 3672 MEDLINE  
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[PMID]:28629229
[Au] Autor:Kádár B; Kocsis B; Tóth Á; Kristóf K; Felso P; Kocsis B; Böddi K; Szabó D
[Ad] Endereço:1 Institute of Medical Microbiology, Semmelweis University , Budapest, Hungary.
[Ti] Título:Colistin resistance associated with outer membrane protein change in Klebsiella pneumoniae and Enterobacter asburiae.
[So] Source:Acta Microbiol Immunol Hung;64(2):217-227, 2017 Jun 01.
[Is] ISSN:1217-8950
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:In this study, outer membrane proteins (OMPs) of colistin-resistant Klebsiella pneumoniae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. pneumoniae sequence type 258 strains as well as one colistin-susceptible E. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. OMP analysis of each strain was performed by microchip method. Matrix-assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) investigation was carried out after separation of OMPs by two-dimensional gel electrophoresis and in-gel digestion. The MALDI-TOF/MS analysis of OMPs in the colistin-susceptible K. pneumoniae found 16 kDa proteins belonging to the LysM domain/BON superfamily, as well as DNA starvation proteins, whereas OmpX and OmpW were detected in the colistin-resistant counterpart strains. OmpC and OmpW were detected in the colistin-susceptible E. asburiae, whereas OmpA and OmpX were identified in the colistin-resistant counterpart. This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains. The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae.
[Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/metabolismo
Colistina/farmacologia
Farmacorresistência Bacteriana
Enterobacter/efeitos dos fármacos
Infecções por Enterobacteriaceae/microbiologia
Infecções por Klebsiella/microbiologia
Klebsiella pneumoniae/efeitos dos fármacos
[Mh] Termos MeSH secundário: Proteínas da Membrana Bacteriana Externa/genética
Enterobacter/genética
Enterobacter/metabolismo
Seres Humanos
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/metabolismo
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); Z67X93HJG1 (Colistin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1556/030.64.2017.017


  3 / 3672 MEDLINE  
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[PMID]:28586403
[Au] Autor:Ghaly TM; Chow L; Asher AJ; Waldron LS; Gillings MR
[Ad] Endereço:Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
[Ti] Título:Evolution of class 1 integrons: Mobilization and dispersal via food-borne bacteria.
[So] Source:PLoS One;12(6):e0179169, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Class 1 integrons have played a major role in the global dissemination of antibiotic resistance. Reconstructing the history of class 1 integrons might help us control further spread of antibiotic resistance by understanding how human activities influence microbial evolution. Here we describe a class 1 integron that represents an intermediate stage in the evolutionary history of clinical integrons. It was embedded in a series of nested transposons, carried on an IncP plasmid resident in Enterobacter, isolated from the surface of baby spinach leaves. Based on the structure of this integron, we present a modified hypothesis for integron assembly, where the ancestral clinical class 1 integron was captured from a betaproteobacterial chromosome to form a Tn402-like transposon. This transposon then inserted into a plasmid-borne Tn21-like ancestor while in an environmental setting, possibly a bacterium resident in the phyllosphere. We suggest that the qacE gene cassette, conferring resistance to biocides, together with the mercury resistance operon carried by Tn21, provided a selective advantage when this bacterium made its way into the human commensal flora via food. The integron characterized here was located in Tn6007, which along with Tn6008, forms part of the larger Tn6006 transposon, itself inserted into another transposable element to form the Tn21-like transposon, Tn6005. This element has previously been described from the human microbiota, but with a promoter mutation that upregulates integron cassette expression. This element we describe here is from an environmental bacterium, and supports the hypothesis that the ancestral class 1 integron migrated into anthropogenic settings via foodstuffs. Selection pressures brought about by early antimicrobial agents, including mercury, arsenic and disinfectants, promoted its initial fixation, the acquisition of promoter mutations, and subsequent dissemination into various species and pathogens.
[Mh] Termos MeSH primário: Elementos de DNA Transponíveis/genética
Farmacorresistência Bacteriana/genética
Doenças Transmitidas por Alimentos/genética
Integrons/genética
[Mh] Termos MeSH secundário: Enterobacter/efeitos dos fármacos
Enterobacter/genética
Enterobacter/patogenicidade
Evolução Molecular
Doenças Transmitidas por Alimentos/microbiologia
Seres Humanos
Mutação
Plasmídeos/genética
Regiões Promotoras Genéticas
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179169


  4 / 3672 MEDLINE  
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[PMID]:28578277
[Au] Autor:Wu YR; Zhang M; Zhong M; Hu Z
[Ad] Endereço:Department of Biology, Shantou University, Shantou, Guangdong 515063, China.
[Ti] Título:Synergistic enzymatic saccharification and fermentation of agar for biohydrogen production.
[So] Source:Bioresour Technol;241:369-373, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nowadays, marine biomass is gradually considered as another utilizable material for the sustainable bioenergy development. In the present study, galactose, the main component of agar polysaccharide, was utilized for the biohydrogen production by Enterobacter sp. CN1. The highest hydrogen yield of 303.2mL/g was obtained in the cultivation media containing 5.87g/L of galactose, together with initial pH of 7.3 and incubation temperature of 36°C, after the response surface methodology (RSM) analysis. After the saccharification process by the agarase (AgaXa) and neoagarobiose hydrolase (NH852), the agar hydrolysate obtained was further applied to generate biohydrogen by strain CN1. Under the synergistic enzymatic saccharification and fermentation process, the production of biohydrogen was obtained to be 5047±228mL/L from 50g/L of agar, resulting in 3.86-fold higher than the control without enzymatic pretreatment.
[Mh] Termos MeSH primário: Ágar
Biocombustíveis
Hidrogênio
[Mh] Termos MeSH secundário: Biomassa
Enterobacter
Fermentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 7YNJ3PO35Z (Hydrogen); 9002-18-0 (Agar)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170605
[St] Status:MEDLINE


  5 / 3672 MEDLINE  
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[PMID]:28559163
[Au] Autor:Sánchez-Calvo JM; de Francisco JL; Torres-Martos E; Alados Arboledas JC; López Prieto MD
[Ad] Endereço:AGS Norte de Cádiz, Hospital SAS de Jerez, Jerez de la Frontera, Cádiz, Spain. Electronic address: jmanuel.sanchez.sspa@juntadeandalucia.es.
[Ti] Título:A cost-saving strategy for processing isolated uropathogens in community-acquired urinary tract infections.
[So] Source:J Microbiol Methods;139:130-134, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total). In the second period, a total 11,728 bacterial isolates were processed, with annual estimated costs of EUR 21,078 and EUR 23,248, respectively (EUR 44,326 total). We included the cost for a laboratory technician (252h worked per year), estimated at EUR 2500 per year. The mean estimated savings were EUR 66,797 (60%).The identification by chromogenic media and antibiotic susceptibility patterns by disk diffusion method was similar to MicroScan in both study periods. Only some isolated Citrobacter spp., Enterobacter spp., Morganella morganii, and Providencia spp. were misidentified. The strategy reported here did not affect the quality of the results and yielded substantial cost savings.
[Mh] Termos MeSH primário: Bactérias/efeitos dos fármacos
Bactérias/isolamento & purificação
Infecções Comunitárias Adquiridas/microbiologia
Testes de Sensibilidade Microbiana/economia
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Compostos Cromogênicos/química
Compostos Cromogênicos/economia
Citrobacter/efeitos dos fármacos
Citrobacter/isolamento & purificação
Citrobacter/patogenicidade
Redução de Custos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/economia
Farmacorresistência Bacteriana
Enterobacter/efeitos dos fármacos
Enterobacter/isolamento & purificação
Enterobacter/patogenicidade
Seres Humanos
Testes de Sensibilidade Microbiana/métodos
Urina/microbiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Chromogenic Compounds)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE


  6 / 3672 MEDLINE  
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[PMID]:28377637
[Au] Autor:Parveez Ahamed AA; Rasheed MU; Peer Muhamed Noorani K; Reehana N; Santhoshkumar S; Mohamed Imran YM; Alharbi NS; Arunachalam C; Alharbi SA; Akbarsha MA; Thajuddin N
[Ad] Endereço:Division of Microbial Biodiversity and Bioenergy, Department of Microbiology, Bharathidasan University, Tiruchirappalli, India.
[Ti] Título:In vitro antibacterial activity of MGDG-palmitoyl from Oscillatoria acuminata NTAPC05 against extended-spectrum ß-lactamase producers.
[So] Source:J Antibiot (Tokyo);70(6):754-762, 2017 Jun.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Extended-spectrum ß-lactamase (ESBL)-producing bacteria pose a big challenge in clinical practices, warranting a new therapeutic strategy. In this study, methanol extract of the marine cyanobacterium Oscillatoria acuminata NTAPC05 was fractionated under bioassay guidance and the fractions were tested against three well-characterized ESBL-producing bacteria Escherichia coli U655, Stenotrophomonas maltophilia B929 and Enterobacter asburiae B938. Out of the four HPLC fractions, fraction 2 showed bactericidal activity against all the three ESBL producers much more efficiently (MIC 100 µg ml ) than the fourth-generation cephalosporin (MIC >125 µg ml ). The active fraction was subjected to time-kill test at concentrations of 1/2 × MIC, 1 × MIC and 2 × MIC, and the results substantiated the bactericidal property of the fraction against the ESBL producers. Spectral analysis revealed monogalactosyldiacylglycerol containing a palmitoyl (MGDG-palmitoyl), being reported for the first time, as the active fraction, and its bactericidal property against ESBL producers was determined. The active fraction appears to damage the bacterial membrane leading to lysis of the cell, as revealed in confocal laser scanning microscopy (CLSM) analysis, that was confirmed in scanning electron microscopic analysis. Cytotoxicity assay revealed the O. acuminata compound to be safe to a normal cell line HEK293 (human embryonic kidney cell). The in silico analysis of MGDG-palmitoyl revealed two successive H-bonding interactions with Leu198 of TEM1 ß-lactamase. Taken together, the MGDG-palmitoyl from O. acuminata NTAPC05 offers potential to develop analogs as a therapeutic for bacteremia caused by ESBL producers.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Enterobacter/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Oscillatoria/metabolismo
Stenotrophomonas maltophilia/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Cefalosporinas/farmacologia
Simulação por Computador
Galactolipídeos/química
Células HEK293
Seres Humanos
Testes de Sensibilidade Microbiana
Microscopia Confocal
Microscopia Eletrônica de Varredura
beta-Lactamases/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0 (Galactolipids); 0 (monogalactosyldiacylglycerol); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.40


  7 / 3672 MEDLINE  
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[PMID]:28365324
[Au] Autor:Xie J; Peters BM; Li B; Li L; Yu G; Xu Z; Shirtliff ME
[Ad] Endereço:School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
[Ti] Título:Clinical features and antimicrobial resistance profiles of important Enterobacteriaceae pathogens in Guangzhou representative of Southern China, 2001-2015.
[So] Source:Microb Pathog;107:206-211, 2017 Jun.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECT: This surveillance aimed to investigate the antimicrobial resistance profiles of Enterobacteriaceae pathogens in Southern China during 2001-2015. METHODS: A total of 6858 Enterobacteriaceae isolates were collected, including 4276 E. coli, 1992 K. pneumoniae and 590 Enterobacter spp. Disk diffusion method and minimum inhibitory concentrations method were used for susceptibility testing, with results interpreted by the CLSI (2015). RESULTS: Urinary tract remained the dominant isolated site among E. coli (49.88%), whereas 53.26% K. pneumoniae and 45.25% Enterobacter spp. were from Sputum. The carbapenems maintained the highest antimicrobial activity (resistance rates <15%), followed by piperacillin-tazobactam and amikacin. Gentle increases were obtained in carbapenems-resistant K. pneumoniae and Enterobacter spp. (eg. from 4.5% to 11.2% and 3.2% to 14.5% in imipenem, repestively). The third-generation cephalosporins showed high and stable resistance among Enterobacteriaceae pathogens during the studied period, with ceftazidime as the most active third-generation cephalosporin against Enterobacteriaceae. Isolates from ICU department showed higher or similar resistance rates among Enterobacteriaceae pathogens compared to other wards. CONCLUSION: Carbapenems are the most potent antibiotic agents against Enterobacteriaceae pathogens. Due to the complicated susceptibility profiles, prescribing guidelines should be based on the knowledge of antibiogram of pathogens.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Enterobacteriaceae/efeitos dos fármacos
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/patogenicidade
[Mh] Termos MeSH secundário: Amicacina/farmacologia
Sangue/microbiologia
Carbapenêmicos/farmacologia
Ceftazidima/farmacologia
Cefalosporinas/farmacologia
China
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/métodos
Enterobacter/efeitos dos fármacos
Enterobacter/isolamento & purificação
Enterobacter/patogenicidade
Escherichia coli/efeitos dos fármacos
Escherichia coli/isolamento & purificação
Escherichia coli/patogenicidade
Seres Humanos
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/isolamento & purificação
Klebsiella pneumoniae/patogenicidade
Testes de Sensibilidade Microbiana/métodos
Ácido Penicilânico/análogos & derivados
Ácido Penicilânico/farmacologia
Piperacilina/farmacologia
Escarro/microbiologia
Sistema Urinário/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems); 0 (Cephalosporins); 157044-21-8 (piperacillin, tazobactam drug combination); 84319SGC3C (Amikacin); 87-53-6 (Penicillanic Acid); 9M416Z9QNR (Ceftazidime); X00B0D5O0E (Piperacillin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE


  8 / 3672 MEDLINE  
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[PMID]:28336135
[Au] Autor:Lee JY; Hong YK; Lee H; Ko KS
[Ad] Endereço:Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea.
[Ti] Título:High prevalence of non-clonal imipenem-nonsusceptible Enterobacter spp. isolates in Korea and their association with porin down-regulation.
[So] Source:Diagn Microbiol Infect Dis;87(1):53-59, 2017 Jan.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the prevalence and clonal distribution of imipenem-nonsusceptible Enterobacter clinical isolates from hospitals in Korea and the contributions of various mechanisms to imipenem nonsusceptibility. The in vitro antimicrobial susceptibility to imipenem of 357 non-duplicated Enterobacter isolates obtained from eight geographically distant tertiary care hospitals in Korea was evaluated. Imipenem-nonsusceptible Enterobacter isolates were genotyped. Additionally, ß-lactamase genes were screened using PCR, and the expression of efflux pump and porin genes was investigated using quantitative RT-PCR. A total of 31 isolates (8.7%) were not susceptible to imipenem. Clonal diversity of 17 imipenem-nonsusceptible E. cloacae isolates was demonstrated by multilocus sequence typing. Fourteen imipenem-nonsusceptible E. aerogenes isolates were found to be distantly genetically related by an ERIC-PCR analysis. Expression levels of porin ompD and ompK35 genes were decreased in all imipenem-nonsusceptible E. cloacae and E. aerogenes isolates. However, only two isolates were found positive for bla and bla genes, and expression of the efflux pump gene, acrB, was not associated with reduced imipenem susceptibility. Imipenem resistance seems to have occurred independently in most of the imipenem-nonsusceptible isolates in this study, and decreased porin expression was found to be the main mechanism underlying this reduced susceptibility to imipenem.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Regulação para Baixo
Enterobacter/efeitos dos fármacos
Infecções por Enterobacteriaceae/microbiologia
Imipenem/farmacologia
Porinas/biossíntese
Resistência beta-Lactâmica
[Mh] Termos MeSH secundário: Enterobacter/classificação
Enterobacter/genética
Enterobacter/isolamento & purificação
Infecções por Enterobacteriaceae/epidemiologia
Genótipo
Seres Humanos
Tipagem Molecular
Prevalência
Reação em Cadeia da Polimerase em Tempo Real
República da Coreia/epidemiologia
Centros de Atenção Terciária
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Porins); 71OTZ9ZE0A (Imipenem); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


  9 / 3672 MEDLINE  
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[PMID]:28328967
[Au] Autor:Harada K; Shimizu T; Mukai Y; Kuwajima K; Sato T; Kajino A; Usui M; Tamura Y; Kimura Y; Miyamoto T; Tsuyuki Y; Ohki A; Kataoka Y
[Ad] Endereço:Department of Veterinary Internal Medicine, Tottori University, Tottori, Japan.
[Ti] Título:Phenotypic and molecular characterization of antimicrobial resistance in Enterobacter spp. isolates from companion animals in Japan.
[So] Source:PLoS One;12(3):e0174178, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The emergence of antimicrobial resistance among Enterobacter spp., including resistance to extended-spectrum cephalosporins (ESC), is of great concern in both human and veterinary medicine. In this study, we investigated the prevalence of antimicrobial resistance among 60 isolates of Enterobacter spp., including E. cloacae (n = 44), E. aerogenes (n = 10), and E. asburiae (n = 6), from clinical specimens of dogs and cats from 15 prefectures in Japan. Furthermore, we characterized the resistance mechanisms harbored by these isolates, including extended-spectrum ß-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR); and assessed the genetic relatedness of ESC-resistant Enterobacter spp. strains by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Antimicrobial susceptibility testing demonstrated the resistance rates to ampicillin (93.3%), amoxicillin-clavulanic acid (93.3%), cefmetazole (93.3%), chloramphenicol (46.7%), ciprofloxacin (43.3%), tetracycline (40.0%), ceftazidime (33.3%), cefotaxime (33.3%), trimethoprim/sulfamethoxazole (28.3%), gentamicin (23.3%), and meropenem (0%). Phenotypic testing detected ESBLs in 16 of 18 ESC-resistant E. cloacae isolates but not in the other species. The most frequent ESBL was CTX-M-15 (n = 8), followed by SHV-12 (n = 7), and CTX-M-3 (n = 1). As for AmpC ß-lactamases, CMY-2 (n = 2) and DHA-1 (n = 2) were identified in ESC-resistant E. cloacae strains with or without ESBLs. All of the ESC-resistant E. cloacae strains also harbored one or two PMQRs, including qnrB (n = 15), aac(6')-Ib-cr (n = 8), and qnrS (n = 2). Based on MLST and PFGE analysis, E. cloacae clones of ST591-SHV-12, ST171-CTX-M-15, and ST121-CTX-M-15 were detected in one or several hospitals. These results suggested intra- and inter-hospital dissemination of E. cloacae clones co-harboring ESBLs and PMQRs among companion animals. This is the first report on the large-scale monitoring of antimicrobial-resistant isolates of Enterobacter spp. from companion animals in Japan.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla/genética
Enterobacter/genética
Animais de Estimação/microbiologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Gatos
Cães
Enterobacter/efeitos dos fármacos
Japão
Testes de Sensibilidade Microbiana/métodos
Tipagem de Sequências Multilocus/métodos
Fenótipo
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174178


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[PMID]:28302074
[Au] Autor:Rosa JF; Rizek C; Marchi AP; Guimaraes T; Miranda L; Carrilho C; Levin AS; Costa SF
[Ad] Endereço:Department of Infectious Diseases, University of São Paulo, Laboratory of Medical Investigation 54 (LIM-54), Hospital Das Clínicas FMUSP, São Paulo, Brazil.
[Ti] Título:Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil.
[So] Source:BMC Microbiol;17(1):69, 2017 Mar 17.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Carbapenems resistance in Enterobacter spp. has increased in the last decade, few studies, however, described the mechanisms of resistance in this bacterium. This study evaluated clonality and mechanisms of carbapenems resistance in clinical isolates of Enterobacter spp. identified in three hospitals in Brazil (Hospital A, B and C) over 7-year. METHODS: Antibiotics sensitivity, pulsed-field gel electrophoresis (PFGE), PCR for carbapenemase and efflux pump genes were performed for all carbapenems-resistant isolates. Outer-membrane protein (OMP) was evaluated based on PFGE profile. RESULTS: A total of 130 isolates of Enterobacter spp were analyzed, 44/105 (41, 9%) E. aerogenes and 8/25 (32,0%) E. cloacae were resistant to carbapenems. All isolates were susceptible to fosfomycin, polymyxin B and tigecycline. KPC was present in 88.6% of E. aerogenes and in all E. cloacae resistant to carbapenems. The carbapenems-resistant E. aerogenes identified in hospital A belonged to six clones, however, a predominant clone was identified in this hospital over the study period. There is a predominant clone in Hospital B and Hospital C as well. The mechanisms of resistance to carbapenems differ among subtypes. Most of the isolates co-harbored blaKPC, blaTEM and /or blaCTX associated with decreased or lost of 35-36KDa and or 39 KDa OMP. The efflux pump AcrAB-TolC gene was only identified in carbapenems-resistant E. cloacae. CONCLUSIONS: There was a predominant clone in each hospital suggesting that cross-transmission of carbapenems-resistant Enterobacter spp. was frequent. The isolates presented multiple mechanisms of resistance to carbapenems including OMP alteration.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Enterobacter/genética
Proteínas de Membrana/genética
Resistência beta-Lactâmica/genética
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Antibacterianos/farmacologia
Brasil
Carbapenêmicos/farmacologia
DNA Bacteriano/genética
Farmacorresistência Bacteriana
Eletroforese em Gel de Campo Pulsado/métodos
Enterobacter/efeitos dos fármacos
Enterobacter/isolamento & purificação
Enterobacter/patogenicidade
Infecções por Enterobacteriaceae/microbiologia
Feminino
Fosfomicina/farmacologia
Genes Bacterianos
Hospitais
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Minociclina/análogos & derivados
Minociclina/farmacologia
Reação em Cadeia da Polimerase
Polimixina B/farmacologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carbapenems); 0 (DNA, Bacterial); 0 (Membrane Proteins); 1404-26-8 (Polymyxin B); 2N81MY12TE (Fosfomycin); 70JE2N95KR (tigecycline); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase); FYY3R43WGO (Minocycline)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-017-0970-1



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