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  1 / 454 MEDLINE  
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[PMID]:29297851
[Au] Autor:Molitor A; James CE; Fanning S; Pagès JM; Davin-Regli A
[Ad] Endereço:1​UMR_MD1, Facultés de Pharmacie and Médecine, Aix-Marseille Univ, Marseille, France.
[Ti] Título:Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes.
[So] Source:J Med Microbiol;67(2):148-159, 2018 Feb.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Several genetic regulators belonging to AraC family are involved in the emergence of MDR isolates of E. aerogenes due to alterations in membrane permeability. Compared with the genetic regulator Mar, RamA may be more relevant towards the emergence of antibiotic resistance. METHODOLOGY: Focusing on the global regulators, Mar and Ram, we compared the amino acid sequences of the Ram repressor in 59 clinical isolates and laboratory strains of E. aerogenes. Sequence types were associated with their corresponding multi-drug resistance phenotypes and membrane protein expression profiles using MIC and immunoblot assays. Quantitative gene expression analysis of the different regulators and their targets (porins and efflux pump components) were performed. RESULTS: In the majority of the MDR isolates tested, ramR and a region upstream of ramA were mutated but marR or marA were unchanged. Expression and cloning experiments highlighted the involvement of the ram locus in the modification of membrane permeability. Overexpression of RamA lead to decreased porin production and increased expression of efflux pump components, whereas overexpression of RamR had the opposite effects. CONCLUSION: Mutations or deletions in ramR, leading to the overexpression of RamA predominated in clinical MDR E. aerogenes isolates and were associated with a higher-level of expression of efflux pump components. It was hypothesised that mutations in ramR, and the self-regulating region proximal to ramA, probably altered the binding properties of the RamR repressor; thereby producing the MDR phenotype. Consequently, mutability of RamR may play a key role in predisposing E. aerogenes towards the emergence of a MDR phenotype.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla/genética
Enterobacter aerogenes/efeitos dos fármacos
Enterobacter aerogenes/genética
Regulação Bacteriana da Expressão Gênica
Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Infecções por Enterobacteriaceae/microbiologia
Genes araC
Loci Gênicos
Seres Humanos
Testes de Sensibilidade Microbiana
Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo
Mutação
Porinas/biossíntese
Porinas/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Multidrug Resistance-Associated Proteins); 0 (Porins); 0 (RamA protein, Enterobacter aerogenes); 0 (Transcription Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000667


  2 / 454 MEDLINE  
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[PMID]:28704737
[Au] Autor:Sharma P; Melkania U
[Ad] Endereço:Department of Environmental Science, GB Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India. Electronic address: preetisharma.doc@gmail.com.
[Ti] Título:Biosurfactant-enhanced hydrogen production from organic fraction of municipal solid waste using co-culture of E. coli and Enterobacter aerogenes.
[So] Source:Bioresour Technol;243:566-572, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of biosurfactants (surfactin and saponin) on the hydrogen production from organic fraction of municipal solid waste (OFMSW) was investigated using co-culture of facultative anaerobes Enterobacter aerogenes and E. coli. The biosurfactants were applied in the concentration ranges of 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 5.0% each. Cumulative hydrogen production (P), maximum hydrogen production rate (Rmax) and lag phases (λ) were analyzed using modified Gompertz model. Results revealed that both the biosurfactants were effective in hydrogen production enhancement. The maximum cumulative hydrogen production of 743.5±14.4ml and 675.6±12.1ml and volumetric hydrogen production of 2.12L /L and 1.93L /L was recorded at 3.5% surfactin and 3.0% saponin respectively. Corresponding highest hydrogen yields were 79.2mlH /gCarbo and 72.0mlH /gCarbo respectively. Lag phase decreased from 12.5±2.0h at control to a minimum of 9.0±2.8h and 9.5±2.1h at 3.5% surfactin and 3.0% saponin respectively. Volatile fatty acid generation was increased with biosurfactants addition.
[Mh] Termos MeSH primário: Biocombustíveis
Enterobacter aerogenes
Escherichia coli
Resíduos Sólidos
[Mh] Termos MeSH secundário: Anaerobiose
Reatores Biológicos
Técnicas de Cocultura
Hidrogênio
Eliminação de Resíduos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Solid Waste); 7YNJ3PO35Z (Hydrogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE


  3 / 454 MEDLINE  
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[PMID]:28562023
[Au] Autor:Pedroso MM; Ely F; Carpenter MC; Mitic N; Gahan LR; Ollis DL; Wilcox DE; Schenk G
[Ad] Endereço:School of Chemistry and Molecular BioSciences, The University of Queensland , St Lucia, QLD 4072, Australia.
[Ti] Título:Mechanistic Insight from Calorimetric Measurements of the Assembly of the Binuclear Metal Active Site of Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes.
[So] Source:Biochemistry;56(26):3328-3336, 2017 Jul 05.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes is a binuclear metallohydrolase with a high affinity for metal ions at its α site but a lower affinity at its ß site in the absence of a substrate. Isothermal titration calorimetry (ITC) has been used to quantify the Co(II) and Mn(II) binding affinities and thermodynamics of the two sites in wild-type GpdQ and two mutants, both in the absence and in the presence of phosphate. Metal ions bind to the six-coordinate α site in an entropically driven process with loss of a proton, while binding at the ß site is not detected by ITC. Phosphate enhances the metal affinity of the α site by increasing the binding entropy and the metal affinity of the ß site by enthalpic (Co) or entropic (Mn) contributions, but no additional loss of protons. Mutations of first- and second-coordination sphere residues at the ß site increase the metal affinity of both sites by enhancing the binding enthalpy. In particular, loss of the hydrogen bond from second-sphere Ser127 to the metal-coordinating Asn80 has a significant effect on the metal binding thermodynamics that result in a resting binuclear active site with high catalytic activity. While structural and spectroscopic data with excess metal ions have indicated a bridging hydroxide in the binuclear GpdQ site, analysis of ITC data here reveals the loss of a single proton in the assembly of this site, indicating that the metal-bound hydroxide nucleophile is formed in the resting inactive mononuclear form, which becomes catalytically competent upon binding the second metal ion.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Cobalto/metabolismo
Enterobacter aerogenes/enzimologia
Manganês/metabolismo
Diester Fosfórico Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Asparagina/química
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Sítios de Ligação
Calorimetria
Domínio Catalítico
Ativação Enzimática
Ligações de Hidrogênio
Cinética
Mutação
Diester Fosfórico Hidrolases/química
Diester Fosfórico Hidrolases/genética
Fósforo/química
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Serina/química
Termodinâmica
Titulometria
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); 27YLU75U4W (Phosphorus); 3G0H8C9362 (Cobalt); 42Z2K6ZL8P (Manganese); 452VLY9402 (Serine); 7006-34-0 (Asparagine); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.46 (glycerophosphodiester phosphodiesterase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.6b01200


  4 / 454 MEDLINE  
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[PMID]:28500888
[Au] Autor:Sharma P; Melkania U
[Ad] Endereço:Department of Environmental Science, GB Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India. Electronic address: preetisharma.doc@gmail.com.
[Ti] Título:Impact of furan derivatives and phenolic compounds on hydrogen production from organic fraction of municipal solid waste using co-culture of Enterobacter aerogenes and E. coli.
[So] Source:Bioresour Technol;239:49-56, 2017 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the present study, the effect of furan derivatives (furfural and 5-hydroxymethylfurfural) and phenolic compounds (vanillin and syringaldehyde) on hydrogen production from organic fraction of municipal solid waste (OFMSW) was investigated using co-culture of facultative anaerobes Enterobacter aerogenes and E. coli. The inhibitors were applied in the concentration ranges of 0.25, 0.5, 1, 2 and 5g/L each. Inhibition coefficients of phenolic compounds were higher than those of furan derivatives and vanillin exhibited maximum inhibition coefficients correspondingly lowest hydrogen yield among all inhibitors. Furfural and 5-hydroxymethylfurfural addition resulted in an average decrease of 26.99% and 37.16% in hydrogen yield respectively, while vanillin and syringaldehyde resulted in 49.40% and 42.26% average decrease in hydrogen yield respectively. Further analysis revealed that Furfural and 5-hydroxymethylfurfural were completely degraded up to concentrations of 1g/L, while vanillin and syringaldehyde were degraded completely up to the concentration of 0.5g/L. Volatile fatty acid generation decreased with inhibitors addition.
[Mh] Termos MeSH primário: Reatores Biológicos
Enterobacter aerogenes
Furanos
Resíduos Sólidos
[Mh] Termos MeSH secundário: Anaerobiose
Técnicas de Cocultura
Escherichia coli
Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Furans); 0 (Solid Waste); 7YNJ3PO35Z (Hydrogen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170514
[St] Status:MEDLINE


  5 / 454 MEDLINE  
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[PMID]:28303845
[Au] Autor:Lu AZ; Shi P; Wang LB; Qian LL; Zhang XB
[Ad] Endereço:Department of Respiratory, Children's Hospital of Fudan University, Shanghai 201102, China.
[Ti] Título:Diagnostic Value of Nasopharyngeal Aspirates in Children with Lower Respiratory Tract Infections.
[So] Source:Chin Med J (Engl);130(6):647-651, 2017 Mar 20.
[Is] ISSN:0366-6999
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The accuracy of nasopharyngeal aspirate (NPA) specimens in detecting lower respiratory pathogens remains controversial. The objective of this study was to evaluate the diagnostic accuracy of aspirates (NPAs) specimen in lower respiratory tract infections (LRTIs) in children. METHODS: The prospective study was designed to collect the data of paired NPAs and bronchoalveolar lavage fluids from children with acute LRTIs from January 2013 to December 2015. All specimens were subjected to pathogen detection: bacterial detection by culture, Mycoplasma pneumoniae (Mp) detection by polymerase chain reaction assay and virus (influenza A and B viruses, parainfluenza virus [PIV] Types 1 and 3, respiratory syncytial virus, and adenovirus) detection by immunofluorescence assay. The diagnostic accuracy analysis of NPAs was stratified by age ≤3 years (n = 194) and >3 years (n = 294). RESULTS: We collected paired specimens from 488 children. The positive rate of pathogen was 61.6%. For Streptococcus pneumoniae, NPA culture had the specificity of 89.9% and negative predictive value of 100% in age ≤3 years, the specificity of 97.2% and negative predictive value of 98.9% in age >3 years. For Mp, the positive predictive values of NPA was 77.4% in children ≤3 years, and 89.1% in children >3 years. For PIV III, NPA specimen had the specificity of 99.8% and negative predictive value of 96.5% in children ≤3 years. For adenovirus, NPA had the specificity of 97.8% and negative predictive value of 98.4% in age ≤3 years, the specificity of 98.9% and negative predictive value of 99.3% in age >3 years. CONCLUSIONS: NPAs are less invasive diagnostic respiratory specimens, a negative NPA result is helpful in "rule out" lower airway infection; however, a positive result does not reliably "rule in" the presence of pathogens.
[Mh] Termos MeSH primário: Nasofaringe/microbiologia
Infecções Respiratórias/diagnóstico
Infecções Respiratórias/microbiologia
[Mh] Termos MeSH secundário: Acinetobacter baumannii/isolamento & purificação
Acinetobacter baumannii/patogenicidade
Adolescente
Criança
Pré-Escolar
Técnicas de Laboratório Clínico/métodos
Enterobacter aerogenes/isolamento & purificação
Enterobacter aerogenes/patogenicidade
Escherichia coli/isolamento & purificação
Escherichia coli/patogenicidade
Feminino
Haemophilus influenzae/isolamento & purificação
Haemophilus influenzae/patogenicidade
Seres Humanos
Lactente
Masculino
Estudos Prospectivos
Pseudomonas aeruginosa/isolamento & purificação
Pseudomonas aeruginosa/patogenicidade
Sensibilidade e Especificidade
Staphylococcus aureus/isolamento & purificação
Staphylococcus aureus/patogenicidade
Streptococcus pneumoniae/isolamento & purificação
Streptococcus pneumoniae/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.4103/0366-6999.201595


  6 / 454 MEDLINE  
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[PMID]:28183602
[Au] Autor:Ahmadzadeh E; Talebnia Rowshan F; Hosseini M
[Ad] Endereço:Faculty of Chemical Engineering, Babol Noshirvani University of Technology, P.O. Box: 484, Postal Code: 47148-71167 Babol, Mazandaran, Iran.
[Ti] Título:A biological method for in-situ synthesis of hydroxyapatite-coated magnetite nanoparticles using Enterobacter aerogenes: Characterization and acute toxicity assessments.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:220-224, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hydroxyapatite (HA)-coated magnetite nanoparticles (MNPs) are being widely investigated for various applications in medical engineering and wastewater treatment. In this work, the MNPs were thoroughly coated by bacterial synthesized HA nanoparticles during biomineralization process using Enterobacter aerogenes. The resulting bacterial-induced precipitate was then calcined at 600°C and investigated with respect to structural characteristics, particle size and magnetic strength by XRD, FT-IR, SEM, EDS, TEM and VSM analyses. The effects of MNPs and HA-coated MNPs (HA-MNPs) on the viability of human MCF-7 cell lines were also investigated via mitochondrial activity test (MTT) and lactate dehydrogenase (LDH) assays. The powder characterization results showed appropriate structural properties for HA-MNPs samples. The particles diameter size of the MNPs and HA-MNPs were in the range of 3-25nm and 20-80nm, respectively. The biologically-synthesized HA-MNPs formed a stable suspension in water while keeping their magnetic property. The saturation magnetization (Ms) of HA-MNPs was measured at ~10emug which was in good agreement with the structural composition of this sample. Finally, the results of the cell lines viability indicated that coating of toxic MNPs via biomineralization was a promising approach in order to synthesize bio-compatible magnetic nanoparticles with suitable physical and chemical structural characteristics. The toxicity level of MNPs was reduced by 10 fold when coated by bacterial-synthesized HA.
[Mh] Termos MeSH primário: Durapatita/toxicidade
Enterobacter aerogenes/efeitos dos fármacos
Nanopartículas de Magnetita/toxicidade
Testes de Toxicidade Aguda
[Mh] Termos MeSH secundário: Morte Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Células MCF-7
Fenômenos Magnéticos
Nanopartículas de Magnetita/ultraestrutura
Tamanho da Partícula
Espectroscopia de Infravermelho com Transformada de Fourier
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Magnetite Nanoparticles); 91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  7 / 454 MEDLINE  
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[PMID]:28098416
[Au] Autor:Borselli D; Blanchet M; Bolla JM; Muth A; Skruber K; Phanstiel O; Brunel JM
[Ad] Endereço:Aix-Marseille Université, IRBA, TMCD2 UMR-MD1, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.
[Ti] Título:Motuporamine Derivatives as Antimicrobial Agents and Antibiotic Enhancers against Resistant Gram-Negative Bacteria.
[So] Source:Chembiochem;18(3):276-283, 2017 Feb 01.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Dihydromotuporamine C and its derivatives were evaluated for their in vitro antimicrobial activities and antibiotic enhancement properties against Gram-negative bacteria and clinical isolates. The mechanism of action of one of these derivatives, MOTU-N44, was investigated against Enterobacter aerogenes by using fluorescent dyes to evaluate outer-membrane depolarization and permeabilization. Its efficiency correlated with inhibition of dye transport, thus suggesting that these molecules inhibit drug transporters by de-energization of the efflux pump rather than by direct interaction of the molecule with the pump. This suggests that depowering the efflux pump provides another strategy to address antibiotic resistance.
[Mh] Termos MeSH primário: Alcaloides/química
Alcaloides/farmacologia
Antibacterianos/farmacologia
Anti-Infecciosos/farmacologia
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Compostos Heterocíclicos com 1 Anel/farmacologia
Poliaminas/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Anti-Infecciosos/química
Enterobacter aerogenes/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Compostos Heterocíclicos com 1 Anel/química
Proteínas de Membrana Transportadoras/metabolismo
Testes de Sensibilidade Microbiana
Poliaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Heterocyclic Compounds, 1-Ring); 0 (MOTU-N44 compound); 0 (Membrane Transport Proteins); 0 (Polyamines); 0 (motuporamine A)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201600532


  8 / 454 MEDLINE  
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[PMID]:28042989
[Au] Autor:Asadi N; Zilouei H
[Ad] Endereço:Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
[Ti] Título:Optimization of organosolv pretreatment of rice straw for enhanced biohydrogen production using Enterobacter aerogenes.
[So] Source:Bioresour Technol;227:335-344, 2017 Mar.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ethanol organosolv pretreated rice straw was used to produce biohydrogen using Enterobacter aerogenes. The effect of temperature (120-180°C), residence time (30-90min), and ethanol concentration (45-75%v/v) on the hydrogen yield, residual biomass, and lignin recovery was investigated using RSM. In contrast to the residual solid and lignin recovery, no considerable trend could be observed for the changes in the hydrogen yield at different treatment severities. The maximum hydrogen yield of 19.73mlg straw was obtained at the ethanol concentration of 45%v/v and 180°C for 30min. Furthermore, the potential amount of biohydrogen was estimated in the top ten rice producing nations using the experimental results. Approximately 355.8kt of hydrogen and 11.3Mt of lignin could globally be produced. Based on a Monte Carlo analysis, the production of biohydrogen from rice straw has the lowest risk in China and the highest in Japan.
[Mh] Termos MeSH primário: Biocombustíveis
Enterobacter aerogenes/metabolismo
Hidrogênio
Oryza/metabolismo
[Mh] Termos MeSH secundário: Biomassa
China
Fermentação
Hidrogênio/química
Hidrogênio/metabolismo
Japão
Lignina/biossíntese
Oryza/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 7YNJ3PO35Z (Hydrogen); 9005-53-2 (Lignin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170103
[St] Status:MEDLINE


  9 / 454 MEDLINE  
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[PMID]:28013136
[Au] Autor:Cheng J; Liu M; Song W; Ding L; Liu J; Zhang L; Cen K
[Ad] Endereço:State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
[Ti] Título:Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation.
[So] Source:Bioresour Technol;227:50-55, 2017 Mar.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of Co γ-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4mLH /(gDWh). The hereditary stability of the E. aerogenes ZJU1 mutant was certified after over ten generations of cultivation. The hydrogen yield of 301mLH /gglucose with the mutant was higher by 81.8% than that of 166mL/gglucose with the wild strain. The peak hydrogen production rate of 27.2mL/(L·h) with the mutant was higher by 40.9% compared with that of 19.3mL/(L·h) with the wild strain. The mutant produced more acetate and butyrate but less ethanol compared with the wild strain during hydrogen fermentation.
[Mh] Termos MeSH primário: Enterobacter aerogenes/metabolismo
Enterobacter aerogenes/efeitos da radiação
[Mh] Termos MeSH secundário: Enterobacter aerogenes/genética
Fermentação
Raios gama
Hidrogênio/química
Mutação/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7YNJ3PO35Z (Hydrogen)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE


  10 / 454 MEDLINE  
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[PMID]:27902398
[Au] Autor:Cabral AB; Maciel MA; Barros JF; Antunes MM; Barbosa de Castro CM; Lopes AC
[Ad] Endereço:2​Universidade Estadual de Ciências da Saúde de Alagoas-UNCISAL, Maceió, Alagoas 57010-300, Brazil 1​Universidade Federal de Pernambuco-UFPE, Recife, Pernambuco 50732-970, Brazil.
[Ti] Título:Clonal spread and accumulation of ß-lactam resistance determinants in Enterobacter aerogenes and Enterobacter cloacae complex isolates from infection and colonization in patients at a public hospital in Recife, Pernambuco, Brazil.
[So] Source:J Med Microbiol;66(1):70-77, 2017 Jan.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Enterobacter aerogenes and Enterobacter cloacae complex are the two species of this genus most involved in healthcare-associated infections that are ESBL and carbapenemase producers. This study characterized, phenotypically and genotypically, 51 isolates of E. aerogenes and E. cloacae complex originating from infection or colonization in patients admitted to a public hospital in Recife, Pernambuco, Brazil, by antimicrobial susceptibility profile, analysis of ß-lactamase genes (blaTEM, blaSHV, blaCTX-M, blaKPC, blaVIM, blaIMP and blaSPM), PCR and DNA sequencing, plasmid profile and ERIC-PCR. In both species, the genes blaTEM, blaCTX-M and blaKPC were detected. The DNA sequencing confirmed the variants blaTEM-1, blaCTX-M-15 and blaKPC-2 in isolates. More than one gene conferring resistance in the isolates, including the detection of the three previously cited genes in strains isolated from infection sites, was observed. The detection of blaCTX-M was more frequent in isolates from infection sites than from colonization. The gene blaKPC predominated in E. cloacae complex isolates obtained from infections; however, in E. aerogenes isolates, it predominated in samples obtained from colonization. A clonal relationship among all of E. aerogenes isolates was detected by ERIC-PCR. The majority of E. cloacae complex isolates presented the same ERIC-PCR pattern. Despite the clonal relation presented by the isolates using ERIC-PCR, different plasmid and resistance profiles and several resistance genes were observed. The clonal dissemination and the accumulation of ß-lactam resistance determinants presented by the isolates demonstrated the ability of E. aerogenes and E. cloacae complex, obtained from colonization and infection, to acquire and maintain different resistance genes.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Enterobacter aerogenes/genética
Enterobacter cloacae/genética
Resistência beta-Lactâmica/genética
beta-Lactamas/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Técnicas de Tipagem Bacteriana
Brasil
Infecção Hospitalar/tratamento farmacológico
Infecção Hospitalar/microbiologia
DNA Bacteriano/genética
Enterobacter aerogenes/efeitos dos fármacos
Enterobacter cloacae/efeitos dos fármacos
Hospitais Públicos
Seres Humanos
Testes de Sensibilidade Microbiana
Análise de Sequência de DNA
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (beta-Lactams); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000398



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