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[PMID]: 26966911 [Au] Autor: Wang Q; Qian L; Jiang S; Cai C; Ma D; Gao P; Li H; Jiang K; Tang M; Hou J; Liu J; Cui W [Ad] Endereço: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, P R China. [Ti] Título: Safety Evaluation of Neo Transgenic Pigs by Studying Changes in Gut Microbiota Using High-Throughput Sequencing Technology. [So] Source: PLoS One;11(3):e0150937, 2016. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The neo (neomycin phosphotransferase) gene is widely used as a selection marker in the production of genetically engineered animals and plants. Recent attention has been focused on safety concerns regarding neo transgene expression. In this study, neo transgenic and non-transgenic piglets were randomly assigned into Group A and Group B to evaluate effects of neo transgene by studying changes in gut microbiota using high-throughput sequencing. Group A pigs were fed a standard diet supplemented with antibiotic neomycin; Group B pigs were fed a standard diet. We examined horizontal transfer of exogenous neo gene using multiplex PCR; and investigated if the presence of secreted NPT II (neo expression product) in the intestine could lead to some protection against neomycin in transgenic pigs by monitoring different patterns of changes in gut microbiota in Group A animals. The unintended effects of neo transgene on gut microbiota were studied in Group B animals. Horizontal gene transfer was not detected in gut microbiota of any transgenic pigs. In Group A, a significant difference was observed between transgenic pigs and non-transgenic pigs in pattern of changes in Proteobacteria populations in fecal samples during and post neomycin feeding. In Group B, there were significant differences in the relative abundance of phyla Firmicutes, Bacteroidetes and Proteobacteria, and genera Lactobacillus and Escherichia-Shigella-Hafnia between transgenic pigs and non-transgenic pigs. We speculate that the secretion of NPT II from transgenic tissues/cells into gut microbiota results in the inhibition of neomycin activity and the different patterns of changes in bacterial populations. Furthermore, the neo gene also leads to unintended effects on gut microbiota in transgenic pigs that were fed with basic diet (not supplemented with neomycin). Thus, our data in this study caution that wide use of the neo transgene in genetically engineered animals should be carefully considered and fully assessed. [Mh] Termos MeSH primário: Animais Geneticamente Modificados/microbiologia Microbioma Gastrointestinal/efeitos dos fármacos Trato Gastrointestinal/microbiologia Canamicina Quinase/genética Suínos/genética [Mh] Termos MeSH secundário: Animais Bacteroidetes/genética Bacteroidetes/isolamento & purificação Farmacorresistência Bacteriana/genética Escherichia coli/genética Escherichia coli/isolamento & purificação Fezes/microbiologia Firmicutes/genética Firmicutes/isolamento & purificação Transferência Genética Horizontal Hafnia/genética Hafnia/isolamento & purificação Sequenciamento de Nucleotídeos em Larga Escala Lactobacillus/genética Lactobacillus/isolamento & purificação Neomicina/farmacologia Proteobactérias/genética Proteobactérias/isolamento & purificação Shigella/genética Shigella/isolamento & purificação Transgenes [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 1404-04-2 (Neomycin); EC 2.7.1.95 (Kanamycin Kinase) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160312 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0150937

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[PMID]: 26433458 [Au] Autor: Hyldgaard M; Meyer RL; Peng M; Hibberd AA; Fischer J; Sigmundsson A; Mygind T [Ad] Endereço: Biofilm Group, Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark; Antimicrobial & Antioxidant Development, Nutrition & Health, DuPont, Edwin Rahrs Vej 38, 8220 Brabrand, Denmark. Electronic address: Morten.hyldgaard@dupont.com. [Ti] Título: Binary combination of epsilon-poly-L-lysine and isoeugenol affect progression of spoilage microbiota in fresh turkey meat, and delay onset of spoilage in Pseudomonas putida challenged meat. [So] Source: Int J Food Microbiol;215:131-42, 2015 Dec 23. [Is] ISSN: 1879-3460 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Proliferation of microbial population on fresh poultry meat over time elicits spoilage when reaching unacceptable levels, during which process slime production, microorganism colony formation, negative organoleptic impact and meat structure change are observed. Spoilage organisms in raw meat, especially Gram-negative bacteria can be difficult to combat due to their cell wall composition. In this study, the natural antimicrobial agents ε-poly-L-lysine (ε-PL) and isoeugenol were tested individually and in combinations for their activities against a selection of Gram-negative strains in vitro. All combinations resulted in additive interactions between ε-PL and isoeugenol towards the bacteria tested. The killing efficiency of different ratios of the two antimicrobial agents was further evaluated in vitro against Pseudomonas putida. Subsequently, the most efficient ratio was applied to a raw turkey meat model system which was incubated for 96 h at spoilage temperature. Half of the samples were challenged with P. putida, and the bacterial load and microbial community composition was followed over time. CFU counts revealed that the antimicrobial blend was able to lower the amount of viable Pseudomonas spp. by one log compared to untreated samples of challenged turkey meat, while the single compounds had no effect on the population. However, the compounds had no effect on Pseudomonas spp. CFU in unchallenged meat. Next-generation sequencing offered culture-independent insight into population diversity and changes in microbial composition of the meat during spoilage and in response to antimicrobial treatment. Spoilage of unchallenged turkey meat resulted in decreasing species diversity over time, regardless of whether the samples received antimicrobial treatment. The microbiota composition of untreated unchallenged meat progressed from a Pseudomonas spp. to a Pseudomonas spp., Photobacterium spp., and Brochothrix thermosphacta dominated food matrix on the expense of low abundance species. We observed a similar shift among the dominant species in meat treated with ε-PL or the antimicrobial blend, but the samples differed markedly in the composition of less abundant species. In contrast, the overall species diversity was constant during incubation of turkey meat challenged with P. putida although the microbiota composition did change over time. Untreated or ε-PL treated samples progressed from a Pseudomonas spp. to a Pseudomonas spp. and Enterobacteriaceae dominated food matrix, while treatment with the antimicrobial blend resulted in increased relative abundance of Hafnia spp., Enterococcaceae, and Photobacterium spp. We conclude that the blend delayed the onset of spoilage of challenged meat, and that all antimicrobial treatments of unchallenged or challenged meat affect the progression of the microbial community composition. Our study confirms that the antimicrobial effects observed in vitro can be extrapolated to a food matrix such as turkey meat. However, it also underlines the consequence of species-to-species variation in susceptibility to antimicrobials, namely that the microbial community change while the CFU remains the same. Addition of antimicrobials may thus prevent the growth of some microorganisms, allowing others to proliferate in their place. [Mh] Termos MeSH primário: Eugenol/análogos & derivados Conservação de Alimentos/métodos Conservantes de Alimentos/farmacologia Carne/microbiologia Polilisina/farmacologia Pseudomonas putida/efeitos dos fármacos Perus/microbiologia [Mh] Termos MeSH secundário: Animais Carga Bacteriana Brochothrix/efeitos dos fármacos Brochothrix/crescimento & desenvolvimento Enterobacteriaceae/efeitos dos fármacos Enterobacteriaceae/crescimento & desenvolvimento Eugenol/farmacologia Microbiologia de Alimentos Hafnia/efeitos dos fármacos Hafnia/crescimento & desenvolvimento Testes de Sensibilidade Microbiana Microbiota/efeitos dos fármacos Photobacterium/efeitos dos fármacos Photobacterium/crescimento & desenvolvimento Pseudomonas putida/crescimento & desenvolvimento [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Food Preservatives); 25104-18-1 (Polylysine); 3T8H1794QW (Eugenol); 5M0MWY797U (isoeugenol) [Em] Mês de entrada: 1606 [Cu] Atualização por classe: 161125 [Lr] Data última revisão: 161125 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151005 [St] Status: MEDLINE

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[PMID]: 25563917 [Au] Autor: Gu Z; Liu Y; Shen L; Liu X; Xiao N; Jiao N; Liu H; Zhou Y; Zhang S [Ad] Endereço: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China. [Ti] Título: Hafnia psychrotolerans sp. nov., isolated from lake water. [So] Source: Int J Syst Evol Microbiol;65(Pt 3):971-4, 2015 Mar. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A psychrotolerant, Gram-stain-negative, motile, aerobic, peritrichous bacterium, strain DJC1-1(T), was isolated from Lake Dajiaco, Tibetan Plateau, China. The strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the Voges-Proskauer reaction and N-acetyl-ß-glucosaminidase. 16S rRNA gene sequence analysis indicated that Hafnia paralvei ATCC 29927(T), Hafnia alvei ATCC 13337(T), Serratia grimesii DSM 30063(T) and Serratia plymuthica DSM 4540(T) were the closest relatives of strain DJC1-1(T), with similarities of 97.76, 96.80, 97.71 and 97.58 %, respectively. The DNA G+C content of strain DJC1-1(T) was 53.9 mol%. The predominant fatty acids were C16 : 0 and C17 : 0 cyclo. Based on these characteristics, strain DJC1-1(T) can be assigned to the genus Hafnia. In DNA-DNA hybridization tests, strain DJC1-1(T) shared 50.6, 35.1, 36.5 and 18.1 % DNA-DNA relatedness with the type strains of H. paralvei, H. alvei, S. grimesii and S. plymuthica, respectively. The growth temperature ranged from 0 to 40 °C, with optimum growth at 15 °C. Physiological and biochemical tests differentiated strain DJC1-1(T) from the type strains of recognized species of the genus Hafnia. Therefore, strain DJC1-1(T) is identified as representing a novel species of the genus Hafnia, for which the name Hafnia psychrotolerans sp. nov. is proposed. The type strain is DJC1-1(T) ( = JCM 30077(T) = CGMCC1.12806(T)). [Mh] Termos MeSH primário: Hafnia/genética Lagos/microbiologia Filogenia [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases China DNA Bacteriano/genética Ácidos Graxos/química Hafnia/crescimento & desenvolvimento Hafnia/isolamento & purificação Dados de Sequência Molecular Hibridização de Ácido Nucleico RNA Ribossômico 16S/genética Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1505 [Cu] Atualização por classe: 150321 [Lr] Data última revisão: 150321 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150108 [St] Status: MEDLINE [do] DOI: 10.1099/ijs.0.000049

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[PMID]: 24768868 [Au] Autor: Drozdzynska A; Pawlicka J; Kubiak P; Kosmider A; Pranke D; Olejnik-Schmidt A; Czaczyk K [Ad] Endereço: Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland. Electronic address: agadro@up.poznan.pl. [Ti] Título: Conversion of glycerol to 1,3-propanediol by Citrobacter freundii and Hafnia alvei - newly isolated strains from the Enterobacteriaceae. [So] Source: N Biotechnol;31(5):402-10, 2014 Sep 25. [Is] ISSN: 1876-4347 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: In this study, nearly 4000 bacterial strains from the family of Enterobacteriaceae isolated from different environments were screened for ability to convert glycerol to 1,3-propanediol (1,3-PD). The aim of the research was to isolate 1,3-PD producers from the natural environment, identify and characterize the best isolates. Three selective media were tested to usefulness in the isolation of bacteria from the family Enterobacteriaceae. Only, 28% of examined isolates could synthesize 1,3-PD from glycerol. 1,3-PD producing bacteria were identified by API 20E tests and 16S rRNA sequences to be Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter freundii and Hafnia alvei. It is the first time, when the fermentation glycerol to 1,3-PD by H. alvei was investigated. The selected strains (C. freundii AD119 and H. alvei AD27) were analyzed on a bioreactor scale under constant pH value 7.0 at temperature of 30°C and 37°C. After 40h in batch fermentation, H. alvei AD27 produced 11.3g/L of 1,3-PD at 37°C. For C. freundii AD119, the best results were obtained at temperature of 30°C. After 24h of fermentation, the 1,3-PD concentration reached above 23 g/L of 1,3-PD. [Mh] Termos MeSH primário: Citrobacter freundii/crescimento & desenvolvimento Glicerol/metabolismo Hafnia/crescimento & desenvolvimento [Mh] Termos MeSH secundário: Citrobacter freundii/isolamento & purificação Hafnia/isolamento & purificação Temperatura Alta Propilenoglicóis [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Propylene Glycols); 5965N8W85T (1,3-propanediol); PDC6A3C0OX (Glycerol) [Em] Mês de entrada: 1505 [Cu] Atualização por classe: 140830 [Lr] Data última revisão: 140830 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140429 [St] Status: MEDLINE

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[PMID]: 24152499 [Au] Autor: Singh M; Ganguli NK; Singh H; Deodhar SD; Sethi S; Sharma M [Ad] Endereço: Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. [Ti] Título: Role of 30 kDa antigen of enteric bacterial pathogens as a possible arthritogenic factor in post-dysenteric reactive arthritis. [So] Source: Indian J Pathol Microbiol;56(3):231-7, 2013 Jul-Sep. [Is] ISSN: 0974-5130 [Cp] País de publicação: India [La] Idioma: eng [Ab] Resumo: BACKGROUND: Reactive arthritis (ReA)/Reiter's syndrome (RS) may be caused as a sequel of infections caused by enteric bacterial pathogens, although the mechanisms through, which different pathogens cause similar disease are not clear. AIM: This study was done to look for the presence and role of any common bacterial antigen among the pathogens isolated from such patients. MATERIALS AND METHODS: A total of 51 patients of ReA and 75 controls (three groups of 25 subjects each: Group 1: Patients who did not develop arthritic complications within 3 months after bacillary dysentery/diarrhea; Group 2: Patients with other arthritic diseases and Group 3: Normal healthy subjects) were included. The isolated enteric pathogens were tested to detect the immunodominant antigens. RESULTS AND CONCLUSIONS: A common 30 kDa antigen was found to be specifically present among seven arthritogenic enteric bacterial strains belonging to three genera, Salmonella, Shigella and Hafnia. Post-dysenteric ReA patients' sera show higher levels of immunoglobulin G, immunoglobulin M and immunoglobulin A antibodies against this antigen as compared to the controls. Lymphocytes of ReA patients recognize this antigen, proliferate and produce interleukin-2 in response to this antigen more than the lymphocytes of controls. 30 kDa antigen may be a common arthritogenic factor associated with post-dysenteric ReA/RS. The association of Hafnia alvei with post-dysenteric ReA is described for the first time. Four cases of mycobacterial ReA had an association with this antigen, suggesting that the arthritogenic antigen of mycobacteria and enteric bacteria may be of a similar nature. [Mh] Termos MeSH primário: Antígenos de Bactérias/imunologia Artrite Reativa/etiologia Disenteria Bacilar/complicações Hafnia/imunologia Salmonella/imunologia Shigella/imunologia [Mh] Termos MeSH secundário: Adulto Anticorpos Antibacterianos/sangue Proliferação Celular Disenteria Bacilar/microbiologia Feminino Seres Humanos Imunoglobulina A/sangue Imunoglobulina G/sangue Interleucina-2/secreção Masculino Linfócitos T/imunologia Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Interleukin-2) [Em] Mês de entrada: 1404 [Cu] Atualização por classe: 131024 [Lr] Data última revisão: 131024 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 131025 [St] Status: MEDLINE [do] DOI: 10.4103/0377-4929.120373

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[PMID]: 21795516 [Au] Autor: Abbott SL; Moler S; Green N; Tran RK; Wainwright K; Janda JM [Ad] Endereço: Microbial Diseases Laboratory, Division of Communicable Disease Control, Center for Infectious Diseases, California Department of Public Health, Richmond, California 94804, USA. [Ti] Título: Clinical and laboratory diagnostic characteristics and cytotoxigenic potential of Hafnia alvei and Hafnia paralvei strains. [So] Source: J Clin Microbiol;49(9):3122-6, 2011 Sep. [Is] ISSN: 1098-660X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A collection of 68 Hafnia strains previously identified to the species level by 16S rRNA gene sequencing were investigated for simple phenotypic properties that could aid in their recognition in the clinical laboratory. Four tests, including malonate utilization, fermentation of salicin and d-arabinose, and expression of ß-glucosidase activity, correctly assigned each strain to either Hafnia alvei or H. paralvei. Antibiotic susceptibility profiles were generated for 35 H. alvei and H. paralvei isolates using Etest strips for 24 antibiotics. All strains were susceptible to aminoglycosides, quinolones, carbapenems, and monobactams. Most of the Hafnia isolates had a colistin MIC of ≥2 µg/ml. Sequencing of an internal ampC gene fragment allowed genotypic differentiation of the two Hafnia species. Approximately 70% of the hafniae tested additionally produced a cytolytic toxin active on Vero cells which may play a role in gastroenteritis. [Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos Infecções por Enterobacteriaceae/diagnóstico Infecções por Enterobacteriaceae/microbiologia Hafnia/classificação Hafnia/fisiologia [Mh] Termos MeSH secundário: Proteínas de Bactérias/genética Biomarcadores Hafnia/genética Hafnia/metabolismo Seres Humanos Testes de Sensibilidade Microbiana Técnicas de Diagnóstico Molecular/métodos beta-Lactamases/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Biomarkers); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases) [Em] Mês de entrada: 1112 [Cu] Atualização por classe: 151119 [Lr] Data última revisão: 151119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 110729 [St] Status: MEDLINE [do] DOI: 10.1128/JCM.00866-11

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[PMID]: 21553084 [Au] Autor: Osuka H; Hitomi S; Koganemaru H; Kaneko T [Ad] Endereço: Department of Infectious Diseases, Tsukuba University Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki 305-8576, Japan. [Ti] Título: A case of bacteremia caused by Hafnia paralvei. [So] Source: J Infect Chemother;17(6):855-7, 2011 Dec. [Is] ISSN: 1437-7780 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The genus Hafnia, a member of the Enterobacteriaceae, is widespread in nature and rarely causes human infection. We describe a case of an 85-year-old Japanese man hospitalized consequent to suspected cholecystitis, in which Hafnia sp. was recovered from the blood culture concomitantly with Enterococcus faecalis. Sequencing of the 16S ribosomal RNA gene sequence and phenotyping with ID 32 E revealed that the recovered Hafnia sp. was considered to be Hafnia alvei genomosp. 2 (ATCC 29927), recently reclassified as Hafnia paralvei. The patient recovered uneventfully with antimicrobial therapies. [Mh] Termos MeSH primário: Bacteriemia/microbiologia Infecções por Enterobacteriaceae/microbiologia Hafnia/isolamento & purificação [Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais Antibacterianos/uso terapêutico Bacteriemia/sangue Bacteriemia/tratamento farmacológico Sequência de Bases Colecistite/sangue Colecistite/microbiologia Diagnóstico Diferencial Infecções por Enterobacteriaceae/sangue Infecções por Enterobacteriaceae/tratamento farmacológico Hafnia/genética Seres Humanos Masculino Dados de Sequência Molecular RNA Ribossômico 16S/química RNA Ribossômico 16S/genética [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1206 [Cu] Atualização por classe: 111214 [Lr] Data última revisão: 111214 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 110510 [St] Status: MEDLINE [do] DOI: 10.1007/s10156-011-0255-9

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[PMID]: 20397936 [Au] Autor: Skurnik D; Nucci A; Ruimy R; Lasocki S; Muller-Serieys C; Montravers P; Andremont A; Courvalin P [Ti] Título: Emergence of carbapenem-resistant Hafnia: the fall of the last soldier. [So] Source: Clin Infect Dis;50(10):1429-31, 2010 May 15. [Is] ISSN: 1537-6591 [Cp] País de publicação: United States [La] Idioma: eng [Mh] Termos MeSH primário: Antibacterianos/farmacologia Carbapenêmicos/farmacologia Infecções por Enterobacteriaceae/microbiologia Hafnia/efeitos dos fármacos Resistência beta-Lactâmica [Mh] Termos MeSH secundário: Proteínas da Membrana Bacteriana Externa/análise Hafnia/química Hafnia/isolamento & purificação Seres Humanos Masculino Testes de Sensibilidade Microbiana Meia-Idade [Pt] Tipo de publicação: CASE REPORTS; LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Bacterial Outer Membrane Proteins); 0 (Carbapenems) [Em] Mês de entrada: 1007 [Cu] Atualização por classe: 100419 [Lr] Data última revisão: 100419 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 100420 [St] Status: MEDLINE [do] DOI: 10.1086/652289

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[PMID]: 19734282 [Au] Autor: Huys G; Cnockaert M; Abbott SL; Janda JM; Vandamme P [Ad] Endereço: Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium. geert.huys@UGent.be [Ti] Título: Hafnia paralvei sp. nov., formerly known as Hafnia alvei hybridization group 2. [So] Source: Int J Syst Evol Microbiol;60(Pt 8):1725-8, 2010 Aug. [Is] ISSN: 1466-5026 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: It has been shown previously, based largely on DNA-DNA hybridizations and partial 16S rRNA gene sequencing, that Hafnia alvei is genotypically heterogeneous and consists of at least two DNA hybridization groups (HGs). In the present study, the taxonomic status of H. alvei HGs 1 and 2 was reassessed. A panel of 24 reference strains and isolates previously assigned to one of the two HGs in H. alvei was subjected to (GTG)5-PCR fingerprinting; this resulted in the delineation of two (GTG)5-PCR clusters in perfect accordance with the respective HG designations. Based on full 16S rRNA gene sequencing of a selection of reference strains, H. alvei HGs 1 and 2 showed internal sequence similarities of 99.8 and 99.5%, respectively. Between the two groups, sequence similarities ranged from 98.8 to 99.1%. Mean DNA-DNA hybridization values of 74.7-99.9% were obtained within each of the two HGs, whereas cross-hybridizations between members of H. alvei HG 1 (including ATCC 13337T) and HG 2 revealed only 32.7-48.7 % DNA-DNA hybridization. Previously published and new phenotypic data revealed that a combination of malonate assimilation and beta-glucosidase activity enabled correct assignment of Hafnia isolates to one of the two HGs. Collectively, taxonomic data from this study confirm that H. alvei comprises at least two taxa at the species level, of which HG 1 corresponds to H. alvei sensu stricto because it includes the type strain ATCC 13337T. Strains formerly classified as members of H. alvei HG 2 represent a novel species, for which the name Hafnia paralvei sp. nov. is proposed; ATCC 29927T (=CDC 4510-73T =LMG 24706T), the former reference strain of H. alvei HG 2, is designated the type strain. [Mh] Termos MeSH primário: Hafnia/classificação Hafnia/isolamento & purificação [Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo DNA Bacteriano/genética DNA Ribossômico/genética Infecções por Enterobacteriaceae/microbiologia Fezes/microbiologia Hafnia/enzimologia Hafnia/genética Seres Humanos Dados de Sequência Molecular Filogenia RNA Ribossômico 16S/genética beta-Glucosidase/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); EC 3.2.1.21 (beta-Glucosidase) [Em] Mês de entrada: 1009 [Cu] Atualização por classe: 100806 [Lr] Data última revisão: 100806 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 090908 [St] Status: MEDLINE [do] DOI: 10.1099/ijs.0.018606-0

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[PMID]: 18257229 [Au] Autor: Gu WN; Huang HQ; Yang PL; Luo HY; Meng K; Wang YR; Yao B [Ad] Endereço: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. [Ti] Título: [Gene cloning, expression and characterization of a novel phytase from Hafnia alvei]. [So] Source: Sheng Wu Gong Cheng Xue Bao;23(6):1017-21, 2007 Nov. [Is] ISSN: 1000-3061 [Cp] País de publicação: China [La] Idioma: chi [Ab] Resumo: A gene appA encoding a novel phytase was firstly cloned from Hafnia alvei by PCR and sequenced. The gene was consisted of 1335 bp, encoding 444 amino acids. The calculated molecular weight of the mature APPA was about 45.2 kD. The gene appA was expressed in E. coli BL21 (DE3). Recombinant APPA was purified and its enzymatic properties were determined. The optimum pH for the enzyme was 4.5 and the optimum temperature was 60 degrees C. The pH stability of r-APPA is good, the relative phytase activity was above 80% after treated in buffers of pH 2.0-10.0. The specific activity of r-APPA is 356.7 U/mg, and the Km value was 0.49 mmol/L and Vmax of 238 U/mg. The enzyme showed resistance to pepsin and trypsin treatment. [Mh] Termos MeSH primário: 6-Fitase/genética Hafnia/enzimologia Hafnia/genética Proteínas Recombinantes/isolamento & purificação [Mh] Termos MeSH secundário: 6-Fitase/biossíntese 6-Fitase/isolamento & purificação Sequência de Aminoácidos Clonagem Molecular Escherichia coli/genética Escherichia coli/metabolismo Concentração de Íons de Hidrogênio Dados de Sequência Molecular Proteínas Recombinantes/biossíntese Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Temperatura Ambiente [Pt] Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Recombinant Proteins); EC 3.1.3.26 (6-Phytase) [Em] Mês de entrada: 0909 [Cu] Atualização por classe: 080208 [Lr] Data última revisão: 080208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 080209 [St] Status: MEDLINE

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