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[PMID]:29117590
[Au] Autor:Gupta P; Kumar V; Usmani Z; Rani R; Chandra A
[Ad] Endereço:Laboratory of Applied Microbiology, Department of Environmental Science & Engineering, Indian Institute of Technology (ISM), Dhanbad 826 004, Jharkhand, India.
[Ti] Título:Phosphate solubilization and chromium (VI) remediation potential of Klebsiella sp. strain CPSB4 isolated from the chromium contaminated agricultural soil.
[So] Source:Chemosphere;192:318-327, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, an effort was made to identify an efficient phosphate solubilizing bacterial strain from chromium contaminated agricultural soils. Based on the formation of a solubilized halo around the colonies on Pikovskaya's agar amended with chromium (VI), 10 strains were initially screened out. Out of 10, strain CPSB4, which showed significantly high solubilization zone at different chromium concentrations, was selected for further study. The strain CPSB4 showed significant plant growth promotion traits with chromium (VI) stress under in-vitro conditions in broth. The plant growth promotion activities of the strain decreased regularly, but were not completely lost with the increase in concentration of chromium up to 200 mg L . On subjected to FT-IR analysis, the presence of the functional group, indicating the organic acid aiding in phosphate solubilization was identified. At an optimal temperature of 30  C and pH 7.0, the strain showed around 93% chromium (VI) reduction under in-vitro conditions in broth study. In soil condition, the maximum chromium (VI) reduction obtained was 95% under in-vitro conditions. The strain CPSB4 was identified as Klebsiella sp. on the basis of morphological, biochemical and 16S rRNA gene sequencing. This study shows that the diverse role of the bacterial strain CPSB4 would be useful in the chromium contaminated soil as a good bioremediation and plant growth promoting agent as well.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cromo/farmacologia
Klebsiella/metabolismo
Fosfatos/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Klebsiella/química
Klebsiella/genética
Klebsiella/isolamento & purificação
Fosfatos/química
Desenvolvimento Vegetal/efeitos dos fármacos
Solo/química
Microbiologia do Solo
Poluentes do Solo/farmacologia
Solubilidade
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphates); 0 (Soil); 0 (Soil Pollutants); 0R0008Q3JB (Chromium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171109
[St] Status:MEDLINE


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[PMID]:29205126
[Au] Autor:Passet V; Brisse S
[Ad] Endereço:Institut Pasteur, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France.
[Ti] Título:Description of Klebsiella grimontii sp. nov.
[So] Source:Int J Syst Evol Microbiol;68(1):377-381, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strains previously identified as Klebsiella oxytocaphylogroup Ko6 were characterized by rpoB, gyrA and rrs gene sequencing, genome-sequence based average nucleotide identity analysis and their biochemical characteristics. rpoB and gyrA sequencing demonstrated that the Ko6 strains formed a well-demarcated sequence cluster related to, but distinct from, Klebsiella oxytoca (which includes strains previously labelled as K. oxytocaphylogroup Ko2) and Klebsiella michiganensis (Ko1). The average nucleotide identity values of Ko6 with K. oxytoca and K. michiganensis were 91.2 and 93.47 %, respectively. The inability to metabolize melezitose differentiated most of the Ko6 strains from K. oxytoca and K. michiganensis. Based on its genetic and phenotypic characteristics, we propose the name Klebsiella grimontii for the Ko6 sequence cluster, with strain 06D021 (=CIP111401 , DSM 105630 ) as the type strain. Strains of Klebsiella grimontii were isolated from human blood cultures, wound infections, antibiotic-associated haemorrhagic colitis and faecal carriage.
[Mh] Termos MeSH primário: Klebsiella/classificação
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Genes Bacterianos
Seres Humanos
Klebsiella/genética
Infecções por Klebsiella/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002517


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[PMID]:28461567
[Au] Autor:Saleh D; Najjar M; Zelic M; Shah S; Nogusa S; Polykratis A; Paczosa MK; Gough PJ; Bertin J; Whalen M; Fitzgerald KA; Slavov N; Pasparakis M; Balachandran S; Kelliher M; Mecsas J; Degterev A
[Ad] Endereço:Medical Scientist Training Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111.
[Ti] Título:Kinase Activities of RIPK1 and RIPK3 Can Direct IFN-ß Synthesis Induced by Lipopolysaccharide.
[So] Source:J Immunol;198(11):4435-4447, 2017 06 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The innate immune response is a central element of the initial defense against bacterial and viral pathogens. Macrophages are key innate immune cells that upon encountering pathogen-associated molecular patterns respond by producing cytokines, including IFN-ß. In this study, we identify a novel role for RIPK1 and RIPK3, a pair of homologous serine/threonine kinases previously implicated in the regulation of necroptosis and pathologic tissue injury, in directing IFN-ß production in macrophages. Using genetic and pharmacologic tools, we show that catalytic activity of RIPK1 directs IFN-ß synthesis induced by LPS in mice. Additionally, we report that RIPK1 kinase-dependent IFN-ß production may be elicited in an analogous fashion using LPS in bone marrow-derived macrophages upon inhibition of caspases. Notably, this regulation requires kinase activities of both RIPK1 and RIPK3, but not the necroptosis effector protein, MLKL. Mechanistically, we provide evidence that necrosome-like RIPK1 and RIPK3 aggregates facilitate canonical TRIF-dependent IFN-ß production downstream of the LPS receptor TLR4. Intriguingly, we also show that RIPK1 and RIPK3 kinase-dependent synthesis of IFN-ß is markedly induced by avirulent strains of Gram-negative bacteria, and , and less so by their wild-type counterparts. Overall, these observations identify unexpected roles for RIPK1 and RIPK3 kinases in the production of IFN-ß during the host inflammatory responses to bacterial infection and suggest that the axis in which these kinases operate may represent a target for bacterial virulence factors.
[Mh] Termos MeSH primário: Interferon beta/biossíntese
Lipopolissacarídeos/imunologia
Macrófagos/imunologia
Proteína Serina-Treonina Quinases de Interação com Receptores/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/imunologia
Bactérias Gram-Negativas/imunologia
Interferon beta/imunologia
Klebsiella/imunologia
Macrófagos/microbiologia
Camundongos
Necrose/imunologia
Fosforilação
Receptor 4 Toll-Like/imunologia
Yersinia/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4); 77238-31-4 (Interferon-beta); EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases); EC 2.7.11.1 (Ripk1 protein, mouse); EC 2.7.11.1 (Ripk3 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180127
[Lr] Data última revisão:
180127
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601717


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[PMID]:28910379
[Au] Autor:Thuy DB; Campbell J; Hoang NVM; Trinh TTT; Duong HTH; Hieu NC; Duy NHA; Hao NV; Baker S; Thwaites GE; Chau NVV; Thwaites CL
[Ad] Endereço:Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programmes, Oxford University Clinical Research Unit (OUCRU), Ho Chi Minh City, Vietnam.
[Ti] Título:A one-year prospective study of colonization with antimicrobial-resistant organisms on admission to a Vietnamese intensive care unit.
[So] Source:PLoS One;12(9):e0184847, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is a paucity of data regarding initial bacterial colonization on admission to Intensive Care Units (ICUs) in low and middle-income countries (LMICs). Patients admitted to ICUs in LMICs are at high-risk of subsequent infection with antimicrobial-resistant organisms (AROs). We conducted a prospective, observational study at the Hospital for Tropical Diseases in Ho Chi Minh City, Vietnam from November 2014 to January 2016 to assess the colonization and antimicrobial susceptibility of Staphylococcus aureus, Escherichia coli, Klebsiella spp., Pseudomonas spp. and Acinetobacter spp. among adult patients within 48 hours of ICU admission. We found the admission colonization prevalence (with at least one of the identified organisms) was 93.7% (785/838) and that of AROs was 63.1% (529/838). The colonization frequency with AROs among patients admitted from the community was comparable to those transferred from other hospitals (62.2% vs 63.8%). Staphylococcus aureus was the most commonly isolated bacteria from nasal swabs (13.1%, 110/838) and the methicillin-resistant Staphylococcus aureus nasal colonization prevalence was 8.6% (72/838). We isolated Escherichia coli from rectal swabs from almost all enrolled patients (88.3%, 740/838) and 52.1% (437/838) of patients were colonized by extended spectrum ß-lactamase producing Escherichia coli. Notably, Klebsiella pneumoniae was the most frequently isolated bacteria from the tracheal swabs (11.8%, 18/153). Vietnamese ICU patients have a high rate of colonization with AROs and are thus at risk of subsequent infections with these organisms if good infection control practices are not in place.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Bactérias/isolamento & purificação
Doenças Transmissíveis/epidemiologia
Doenças Transmissíveis/microbiologia
Farmacorresistência Bacteriana
[Mh] Termos MeSH secundário: Acinetobacter/efeitos dos fármacos
Acinetobacter/isolamento & purificação
Adulto
Bactérias/classificação
Escherichia coli/efeitos dos fármacos
Escherichia coli/isolamento & purificação
Feminino
Seres Humanos
Unidades de Terapia Intensiva
Klebsiella/efeitos dos fármacos
Klebsiella/isolamento & purificação
Masculino
Meia-Idade
Nariz/microbiologia
Prevalência
Estudos Prospectivos
Pseudomonas/efeitos dos fármacos
Pseudomonas/isolamento & purificação
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/isolamento & purificação
Vietnã/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184847


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[PMID]:28845933
[Au] Autor:Kogan MI; Naboka YL; Bedzhanyan SK; Mitusova EV; Gudima IA; Morgun PP; Vasileva LI
[Ad] Endereço:RostSMU of Minzdrav of Russia, Rostov on Don, Russia.
[Ti] Título:[Is bacteriological testing of bladder urine informative in acute obstructive pyelo- nephritis?]
[So] Source:Urologiia;(3):10-15, 2017 Jul.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The problem of the etiology and pathogenesis of acute obstructive pyelonephritis (OOP) remains one of the challenging issues of modern urology. Etiological agents of pyelonephritis can be both gram-negative and gram-positive opportunistic bacteria mostly belonging to the normal flora in humans. The generally accepted diagnostic work-up involves a bacteriological testing of not pelvic urine, but of bladder urine collected by a transurethral catheter or midstream specimens of urine collected from the patients. The aim of our study was to compare the microbiota of bladder and pelvic urine in patients with OOP. MATERIALS AND METHODS: The study comprised 72 sequentially selected patients (12 men and 60 women) with OOP associated with ureteral stones. Mean age of patients was 53.7+/-0.5 years. All patients underwent bacteriological examination of the bladder urine collected by a transurethral catheter and pelvic urine obtained after relieving stone-related ureteral obstruction. Urinary diversion was performed using j-j stent and PCN in 64 and 8 patients, respectively. Preoperative prophylactic antibiotics were administered routinely. Bacteriological testing of urine was carried out using an extended set (9-10) of culture media. Empirical antibiotic therapy was initiated only after the restoration of urine outflow from the kidney and continued for 5-6 days until the availability of bacteriological testing results. RESULTS: Levels of bacteriuria with Enterobacteria, gram-positive pathogens and NAB in two urine samples did not differ significantly (p>0.05). There was a wide range of bacteriuria from 101 to 106 CFU/ml of most microorganisms except @Proteus spp., S. aureus. In bladder urine, the rates of bacteriuria of more or equal 104 CFU/ml for E. coli, Klebsiella spp. and Proteus spp. were 90.9%, 72.7% and 100.0%, respectively. For the remaining microorganisms, predominant bacteriuria was less or equal 103 CFU/ml. In pelvic urine, the rates of bacteriuria of more or equal 104 CFU/ml for E. coli, Klebsiella spp. and Proteus spp. was 71.8%, 40.0% and 66.7%, respectively. Other uropathogens in the pelvic urine mainly had a bacterial count of less or equal 103 CFU/ml. Only the concentration of Corynebacterium spp. in the pelvic urine significantly (p=0.023) differed from that of the bladder urine. There were no significant differences between microbiota of bladder and pelvic urine depending on duration of OOP except higher rates of Corynebacterium spp. in the bladder urine.
[Mh] Termos MeSH primário: Pielonefrite/urina
Infecções Urinárias/urina
Urina/microbiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Antibacterianos/administração & dosagem
Bacteriúria/prevenção & controle
Bacteriúria/urina
Corynebacterium/isolamento & purificação
Escherichia coli/isolamento & purificação
Feminino
Seres Humanos
Klebsiella/isolamento & purificação
Masculino
Meia-Idade
Proteus/isolamento & purificação
Pielonefrite/prevenção & controle
Infecções Urinárias/prevenção & controle
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


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[PMID]:28619105
[Au] Autor:Tran GM; Ho-Le TP; Ha DT; Tran-Nguyen CH; Nguyen TSM; Pham TTN; Nguyen TA; Nguyen DA; Hoang HQ; Tran NV; Nguyen TV
[Ad] Endereço:ICU, Gia Dinh People's Hospital, 1 No Trang Long Street, Binh Thanh District, Ho Chi Minh City, Vietnam. giangbacsyicu@gmail.com.
[Ti] Título:Patterns of antimicrobial resistance in intensive care unit patients: a study in Vietnam.
[So] Source:BMC Infect Dis;17(1):429, 2017 Jun 15.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Antimicrobial resistance has emerged as a major concern in developing countries. The present study sought to define the pattern of antimicrobial resistance in ICU patients with ventilator-associated pneumonia. METHODS: Between November 2014 and September 2015, we enrolled 220 patients (average age ~ 71 yr) who were admitted to ICU in a major tertiary hospital in Ho Chi Minh City, Vietnam. Data concerning demographic characteristics and clinical history were collected from each patient. The Bauer-Kirby disk diffusion method was used to detect the antimicrobial susceptibility. RESULTS: Antimicrobial resistance was commonly found in ceftriaxone (88%), ceftazidime (80%), ciprofloxacin (77%), cefepime (75%), levofloxacin (72%). Overall, the rate of antimicrobial resistance to any drug was 93% (n = 153/164), with the majority (87%) being resistant to at least 2 drugs. The three commonly isolated microorganisms were Acinetobacter (n = 75), Klebsiella (n = 39), and Pseudomonas aeruginosa (n = 29). Acinetobacter baumannii were virtually resistant to ceftazidime, ceftriaxone, piperacilin, imipenem, meropenem, ertapenem, ciprofloxacin and levofloxacin. High rates (>70%) of ceftriaxone and ceftazidime-resistant Klebsiella were also observed. CONCLUSION: These data indicated that critically ill patients on ventilator in Vietnam were at disturbingly high risk of antimicrobial resistance. The data also imply that these Acinetobacter, Klebsiella, and Pseudomonas aeruginosa and multidrug resistance pose serious therapeutic problems in ICU patients. A concerted and systematic effort is required to rapidly identify high risk patients and to reduce the burden of antimicrobial resistance in developing countries.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Farmacorresistência Bacteriana/efeitos dos fármacos
Unidades de Terapia Intensiva
Pneumonia Associada à Ventilação Mecânica/microbiologia
[Mh] Termos MeSH secundário: Infecções por Acinetobacter/tratamento farmacológico
Infecções por Acinetobacter/microbiologia
Acinetobacter baumannii/efeitos dos fármacos
Acinetobacter baumannii/isolamento & purificação
Idoso
Antibacterianos/farmacologia
Ceftazidima/farmacologia
Ceftazidima/uso terapêutico
Ciprofloxacino/farmacologia
Ciprofloxacino/uso terapêutico
Feminino
Seres Humanos
Imipenem/farmacologia
Klebsiella/efeitos dos fármacos
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Pneumonia Associada à Ventilação Mecânica/tratamento farmacológico
Pneumonia Associada à Ventilação Mecânica/mortalidade
Infecções por Pseudomonas/tratamento farmacológico
Infecções por Pseudomonas/microbiologia
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/isolamento & purificação
Vietnã
beta-Lactamas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (beta-Lactams); 5E8K9I0O4U (Ciprofloxacin); 71OTZ9ZE0A (Imipenem); 9M416Z9QNR (Ceftazidime); G32F6EID2H (ertapenem)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2529-z


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[PMID]:28524019
[Au] Autor:Schönborn S; Wente N; Paduch JH; Krömker V
[Ad] Endereço:Faculty II,Department of Microbiology,University of Applied Sciences and Arts Hannover,Heisterbergallee 12,30453 Hannover,Germany.
[Ti] Título:In vitro ability of mastitis causing pathogens to form biofilms.
[So] Source:J Dairy Res;84(2):198-201, 2017 May.
[Is] ISSN:1469-7629
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This Research Communication describes the study of in vitro biofilm formation of mastitis causing pathogens. Biofilms are communities of bacteria that are attached to a surface and to each other and are embedded in a self-produced matrix of extracellular polymeric substances. Biofilm formation is an important virulence factor that may result in recurrent or persistent udder infections and treatment failure through increased resistance to antibiotics and protection against host defences. In the present study 252 bacterial isolates from milk samples from bovine udder quarters with intramammary infections were examined with Congo Red agar (CRA) method and tube method (TM) for their ability to form biofilms. Both tests revealed a high number of biofilm-positive strains. Literature reports that the cure rates for Staphylococcus aureus infected udders are lower (27%) in comparison to cure rates of Streptococcus uberis (64-81%) or coagulase-negative staphylococci (CNS) mastitis (80-90%). The findings of the present study suggest that biofilm formation is not the main factor for the differences in cure rates of the various bacteria genera, because all tested pathogen groups showed a similarly high proportion of biofilm formation. Further research is needed to detect microbial biofilms on bovine udder epithelia.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos Bacterianos
Biofilmes/crescimento & desenvolvimento
Mastite Bovina/microbiologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Escherichia coli/fisiologia
Feminino
Klebsiella/fisiologia
Glândulas Mamárias Animais/microbiologia
Mastite Bovina/tratamento farmacológico
Mastite Bovina/prevenção & controle
Técnicas Microbiológicas/métodos
Leite/microbiologia
Staphylococcus/fisiologia
Staphylococcus aureus/fisiologia
Streptococcus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1017/S0022029917000218


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[PMID]:28425599
[Au] Autor:Janecek S; Majzlová K; Svensson B; MacGregor EA
[Ad] Endereço:Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia.
[Ti] Título:The starch-binding domain family CBM41-An in silico analysis of evolutionary relationships.
[So] Source:Proteins;85(8):1480-1492, 2017 Aug.
[Is] ISSN:1097-0134
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Within the CAZy database, there are 81 carbohydrate-binding module (CBM) families. A CBM represents a non-catalytic domain in a modular arrangement of glycoside hydrolases (GHs). The present in silico study has been focused on starch-binding domains from the family CBM41 that are usually part of pullulanases from the α-amylase family GH13. Currently there are more than 1,600 sequences classified in the family CBM41, almost exclusively from Bacteria, and so a study was undertaken in an effort to divide the members into relevant groups (subfamilies) and also to contribute to the evolutionary picture of family CBM41. The CBM41 members adopt a ß-sandwich fold (∼100 residues) with one carbohydrate-binding site formed by the side-chains of three aromatic residues that interact with carbohydrate. The family CBM41 can be divided into two basic subdivisions, distinguished from each other by a characteristic sequence pattern or motif of the three essential aromatics as follows: (i) "W-W-∼10aa-W" (the so-called Streptococcus/Klebsiella-type); and (ii) "W-W-∼30aa-W" (Thermotoga-type). Based on our bioinformatics analysis it is clear that the first and second positions of the motif can be occupied by aromatic residues (Phe, Tyr, His) other than tryptophan, resulting in the existence of six different carbohydrate-binding CBM41 groups, that reflect mostly differences in taxonomy, but which should retain the ability to bind an α-glucan. In addition, three more groups have been proposed that, although lacking the crucial aromatic motif, could possibly employ other residues from remaining parts of their sequence for binding carbohydrate. Proteins 2017; 85:1480-1492. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Evolução Molecular
Glicosídeo Hidrolases/química
Filogenia
Receptores de Superfície Celular/química
alfa-Amilases/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sítios de Ligação
Biologia Computacional
Bases de Dados de Proteínas
Glicosídeo Hidrolases/metabolismo
Klebsiella/química
Klebsiella/classificação
Klebsiella/metabolismo
Modelos Moleculares
Família Multigênica
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Isoformas de Proteínas/química
Isoformas de Proteínas/metabolismo
Estrutura Terciária de Proteína
Receptores de Superfície Celular/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Streptococcus/química
Streptococcus/classificação
Streptococcus/metabolismo
Especificidade por Substrato
Thermotoga maritima/química
Thermotoga maritima/classificação
Thermotoga maritima/metabolismo
alfa-Amilases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Isoforms); 0 (Receptors, Cell Surface); 0 (saccharide-binding proteins); EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.41 (pullulanase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1002/prot.25309


  9 / 4630 MEDLINE  
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[PMID]:28362303
[Au] Autor:Lin H; Zhu X; Wang Y; Yu X
[Ad] Endereço:Institute of Urban Environment, Chinese Academy of Science, Xiamen 361021, China E-mail: xyu@iue.ac.cn.
[Ti] Título:Effect of sodium hypochlorite on typical biofilms formed in drinking water distribution systems.
[So] Source:J Water Health;15(2):218-227, 2017 Apr.
[Is] ISSN:1477-8920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human health and biological safety problems resulting from urban drinking water pipe network biofilms pollution have attracted wide concern. Despite the inclusion of residual chlorine in drinking water distribution systems supplies, the bacterium is a recalcitrant human pathogen capable of forming biofilms on pipe walls and causing health risks. Typical drinking water bacterial biofilms and their response to different concentrations of chlorination was monitored. The results showed that the four bacteria all formed single biofilms susceptible to sodium hypochlorite. After 30 min disinfection, biomass and cultivability decreased with increasing concentration of disinfectant but then increased in high disinfectant doses. PMA-qPCR results indicated that it resulted in little cellular damage. Flow cytometry analysis showed that with increasing doses of disinfectant, the numbers of clusters increased and the sizes of clusters decreased. Under high disinfectant treatment, EPS was depleted by disinfectant and about 0.5-1 mg/L of residual chlorine seemed to be appropriate for drinking water treatment. This research provides an insight into the EPS protection to biofilms. Resistance of biofilms against high levels of chlorine has implications for the delivery of drinking water.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos Bacterianos
Biofilmes/efeitos dos fármacos
Desinfetantes/farmacologia
Água Potável/microbiologia
Hipoclorito de Sódio/farmacologia
[Mh] Termos MeSH secundário: Bactérias/efeitos dos fármacos
Desinfecção
Flavobacterium/fisiologia
Klebsiella/fisiologia
Pseudomonas/fisiologia
Sphingomonas/fisiologia
Purificação da Água
Abastecimento de Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (Drinking Water); DY38VHM5OD (Sodium Hypochlorite)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.2166/wh.2017.141


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[PMID]:28346512
[Au] Autor:Bhuyan GS; Hossain MA; Sarker SK; Rahat A; Islam MT; Haque TN; Begum N; Qadri SK; Muraduzzaman AK; Islam NN; Islam MS; Sultana N; Jony MH; Khanam F; Mowla G; Matin A; Begum F; Shirin T; Ahmed D; Saha N; Qadri F; Mannoor K
[Ad] Endereço:Infectious diseases Laboratory, Institute for Developing Science and Health Initiatives, Mohakhali, Dhaka, Bangladesh.
[Ti] Título:Bacterial and viral pathogen spectra of acute respiratory infections in under-5 children in hospital settings in Dhaka city.
[So] Source:PLoS One;12(3):e0174488, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The study aimed to examine for the first time the spectra of viral and bacterial pathogens along with the antibiotic susceptibility of the isolated bacteria in under-5 children with acute respiratory infections (ARIs) in hospital settings of Dhaka, Bangladesh. Nasal swabs were collected from 200 under-five children hospitalized with clinical signs of ARIs. Nasal swabs from 30 asymptomatic children were also collected. Screening of viral pathogens targeted ten respiratory viruses using RT-qPCR. Bacterial pathogens were identified by bacteriological culture methods and antimicrobial susceptibility of the isolates was determined following CLSI guidelines. About 82.5% (n = 165) of specimens were positive for pathogens. Of 165 infected cases, 3% (n = 6) had only single bacterial pathogens, whereas 43.5% (n = 87) cases had only single viral pathogens. The remaining 36% (n = 72) cases had coinfections. In symptomatic cases, human rhinovirus was detected as the predominant virus (31.5%), followed by RSV (31%), HMPV (13%), HBoV (11%), HPIV-3 (10.5%), and adenovirus (7%). Streptococcus pneumoniae was the most frequently isolated bacterial pathogen (9%), whereas Klebsiella pneumaniae, Streptococcus spp., Enterobacter agglomerans, and Haemophilus influenzae were 5.5%, 5%, 2%, and 1.5%, respectively. Of 15 multidrug-resistant bacteria, a Klebsiella pneumoniae isolate and an Enterobacter agglomerans isolate exhibited resistance against more than 10 different antibiotics. Both ARI incidence and predominant pathogen detection rates were higher during post-monsoon and winter, peaking in September. Pathogen detection rates and coinfection incidence in less than 1-year group were significantly higher (P = 0.0034 and 0.049, respectively) than in 1-5 years age group. Pathogen detection rate (43%) in asymptomatic cases was significantly lower compared to symptomatic group (P<0.0001). Human rhinovirus, HPIV-3, adenovirus, Streptococcus pneumonia, and Klebsiella pneumaniae had significant involvement in coinfections with P values of 0.0001, 0.009 and 0.0001, 0.0001 and 0.001 respectively. Further investigations are required to better understand the clinical roles of the isolated pathogens and their seasonality.
[Mh] Termos MeSH primário: Coinfecção/diagnóstico
Klebsiella/isolamento & purificação
Infecções Respiratórias/diagnóstico
Rhinovirus/isolamento & purificação
Streptococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Doença Aguda
Bangladesh
Pré-Escolar
Coinfecção/microbiologia
Coinfecção/virologia
Feminino
Seres Humanos
Lactente
Masculino
Infecções Respiratórias/microbiologia
Infecções Respiratórias/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174488



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