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[PMID]: 29458684 [Au] Autor: Wise MG; Horvath E; Young K; Sahm DF; Kazmierczak KM [Ad] Endereço: 1​International Health Management Associates, Schaumburg, Illinois, USA. [Ti] Título: Global survey of Klebsiella pneumoniae major porins from ertapenem non-susceptible isolates lacking carbapenemases. [So] Source: J Med Microbiol;67(3):289-295, 2018 Mar. [Is] ISSN: 1473-5644 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: PURPOSE: To understand the diversity of porin disruption in Klebsiella pneumoniae, the major outer membrane protein (OMP) porins, OmpK35 and OmpK36, were examined in a set of isolates that did not harbour traditional carbapenem-hydrolysing enzymes, but nevertheless tested non-susceptible to ertapenem. METHODS: A world-wide collection of Klebsiella pneumoniae isolates that were part of the Study for Monitoring Antimicrobial Resistance Trends (SMART) surveillance project over the years 2008-2014 were characterised with regard to their ß-lactamase gene carriage and potential permeability defects. Four hundred and eighty-seven isolates that did not carry carbapenemase genes, but were non-susceptible to ertapenem, were investigated by sequence analysis of the genes encoding OmpK35 and OmpK36. Isolates without obvious genetic lesions in either major porin gene were further examined by outer membrane protein SDS-PAGE. RESULTS: The majority of isolates, 83.0 % (404/487), exhibited clear genetic disruption in either or both of the ompK35 and ompK36 genes. Among the proportion of the collection with the highest ertapenem MIC value (>4 mg l ), 60.5 % (115/190) showed mutation in both porin genes. Isolates without obvious genetic mutations were examined by SDS-PAGE, and 90.4 % (75/83) were found to lack or show altered expression of at least one of the major OMPs when compared to an ertapenem sensitive control strain. CONCLUSION: This study illustrates that porin deficiency in Klebsiella pneumoniae is a widespread phenomenon, and in combination with ESBLs and/or AmpC enzymes, likely accounts for the elevated ertapenem MICs observed in this study. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Proteínas de Bactérias/genética Klebsiella pneumoniae/genética Porinas/genética beta-Lactamas/farmacologia [Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo Carbapenêmicos/farmacologia DNA Bacteriano/genética Eletroforese em Gel de Poliacrilamida Seres Humanos Infecções por Klebsiella/epidemiologia Infecções por Klebsiella/microbiologia Klebsiella pneumoniae/efeitos dos fármacos Klebsiella pneumoniae/isolamento & purificação Klebsiella pneumoniae/metabolismo Testes de Sensibilidade Microbiana Mutação beta-Lactamases/genética beta-Lactamases/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carbapenems); 0 (DNA, Bacterial); 0 (OmpK35 porin, Klebsiella pneumoniae); 0 (OmpK36 protein, Klebsiella pneumoniae); 0 (Porins); 0 (beta-Lactams); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase); G32F6EID2H (ertapenem) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180308 [Lr] Data última revisão: 180308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180221 [St] Status: MEDLINE [do] DOI: 10.1099/jmm.0.000691

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[PMID]: 29458540 [Au] Autor: Li Y; Zhang L; Zhou Y; Zhang Z; Zhang X [Ad] Endereço: 1​Department of Immunology, College of Preclinical Medicine, Southwest Medical University, Luzhou 646000, Sichuan, PR China. [Ti] Título: Survival of bactericidal antibiotic treatment by tolerant persister cells of Klebsiella pneumoniae. [So] Source: J Med Microbiol;67(3):273-281, 2018 Mar. [Is] ISSN: 1473-5644 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: PURPOSE: Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infections. In this study, we aimed to investigate the persister subpopulation of K. pneumoniae. METHODOLOGY: The presence of persisters in K. pneumoniae was determined by treatment with high concentrations of antibiotics, used alone or in combination. The effect of low level of antibiotics on persister formation was investigated by pre-exposure of cells to antibiotics with low concentrations followed by higher doses. The dependence of persister levels on growth phase was determined by measuring the survival ability of cells along the growth stages upon exposure to a high concentration of antibiotic. Analysis on persister type was carried out by persister elimination assays.Results/Key findings. We show that K. pneumoniae produces high levels of tolerant persister cells to survive treatment by a variety of high concentrations of bactericidal antibiotics and persister formation is prevalent among K. pneumoniae clinical strains. Besides, we find that persister cells can be induced by low concentrations of antibiotics. Finally, we provide evidence that persister formation is growth phase-dependent and Type II persisters dominate the persister subpopulation during the entire exponential phase of K. pneumoniae. CONCLUSION: Our study describes the formation of tolerant persister cells that allow survival of treatment by high concentrations of antibiotics in K. pneumoniae. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Klebsiella pneumoniae/efeitos dos fármacos Klebsiella pneumoniae/fisiologia Viabilidade Microbiana/efeitos dos fármacos [Mh] Termos MeSH secundário: Contagem de Colônia Microbiana Farmacorresistência Bacteriana Múltipla Tolerância a Medicamentos Seres Humanos Infecções por Klebsiella/microbiologia Klebsiella pneumoniae/crescimento & desenvolvimento Testes de Sensibilidade Microbiana [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180308 [Lr] Data última revisão: 180308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180221 [St] Status: MEDLINE [do] DOI: 10.1099/jmm.0.000680

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[PMID]: 29465589 [Au] Autor: Geng TT; Xu X; Huang M [Ad] Endereço: Department of General Intensive Care Unit, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, People's Republic of China. [Ti] Título: High-dose tigecycline for the treatment of nosocomial carbapenem-resistant Klebsiella pneumoniae bloodstream infections: A retrospective cohort study. [So] Source: Medicine (Baltimore);97(8):e9961, 2018 Feb. [Is] ISSN: 1536-5964 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infection (BSI) has become increasingly frequent threat recently, especially in the intensive care unit (ICU). High-dose tigecycline (TGC) regimen is proposed due to the limitation of treatment options. We investigated the efficacy and safety of high-dose TGC combination regimens for treating CRKP BSI. Furthermore, the risk factors for mortality were also determined.This was a single center retrospective cohort study conducted from 2014 to 2016. A total of 40 patients with nosocomial CRKP BSI admitted to the ICU were included; they were classified into two groups according to the treatment regimens with high-dose TGC (HD group) or not (non-HD group). In-hospital mortality rates and microbiologic responses from both groups were reviewed and compared. Besides, the survival and non-survival groups were compared to identify the risk factors of mortality.Twenty-three patients constituted the HD group (high-dosage TGC regimen was administered as 200 mg loading dose followed by 100 mg every 12 h) and 17 patients constituted the non-HD group (standard dose TGC therapy as 100 mg loading dose followed by 50 mg every 12 h and other antibiotics). The in-hospital mortality was 52.2% in the HD group and 76.5% in the non-HD group (P = .117). The Kaplan-Meier test showed significantly longer survival times in the HD group (mean: 83 days vs 28 days; P = .027). Microbiological eradication was observed in 13 patients (56.5%) in the HD group and 6 patients (36.3%) in the non-HD group (P = .184). A smaller fraction of patients in the HD group were subjected to vasoactive therapy (52.2% vs 88.2%; P = .016) compared to the non-HD group. There was no significant difference in the manifestation of adverse effects between the two groups. In the multivariate analysis, multiple organ dysfunction syndrome (MODS), vasoactive therapy, and exposure to carbapenems were regarded as the independent predictors of mortality.A therapeutic regimen consisting of a high dose of TGC was associated with significantly longer survival time and numerically lower mortality in CRKP BSI. Adverse events were not increased with the double dose therapy. [Mh] Termos MeSH primário: Antibacterianos/administração & dosagem Bacteriemia/tratamento farmacológico Infecção Hospitalar/tratamento farmacológico Infecções por Klebsiella/tratamento farmacológico Minociclina/análogos & derivados [Mh] Termos MeSH secundário: Idoso Bacteriemia/microbiologia Bacteriemia/mortalidade Enterobacteriáceas Resistentes a Carbapenêmicos/efeitos dos fármacos Infecção Hospitalar/microbiologia Infecção Hospitalar/mortalidade Feminino Mortalidade Hospitalar Seres Humanos Estimativa de Kaplan-Meier Infecções por Klebsiella/mortalidade Klebsiella pneumoniae/efeitos dos fármacos Masculino Meia-Idade Minociclina/administração & dosagem Estudos Retrospectivos Resultado do Tratamento [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 70JE2N95KR (tigecycline); FYY3R43WGO (Minocycline) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180302 [Lr] Data última revisão: 180302 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 180222 [St] Status: MEDLINE [do] DOI: 10.1097/MD.0000000000009961

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[PMID]: 29348071 [Au] Autor: Akhter S; Lund BA; Ismael A; Langer M; Isaksson J; Christopeit T; Leiros HS; Bayer A [Ad] Endereço: Department of Chemistry, Faculty of Science and Technology, UiT- The Arctic University of Norway, N-9037 Tromsø, Norway. [Ti] Título: A focused fragment library targeting the antibiotic resistance enzyme - Oxacillinase-48: Synthesis, structural evaluation and inhibitor design. [So] Source: Eur J Med Chem;145:634-648, 2018 Feb 10. [Is] ISSN: 1768-3254 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: ß-Lactam antibiotics are of utmost importance when treating bacterial infections in the medical community. However, currently their utility is threatened by the emergence and spread of ß-lactam resistance. The most prevalent resistance mechanism to ß-lactam antibiotics is expression of ß-lactamase enzymes. One way to overcome resistance caused by ß-lactamases, is the development of ß-lactamase inhibitors and today several ß-lactamase inhibitors e.g. avibactam, are approved in the clinic. Our focus is the oxacillinase-48 (OXA-48), an enzyme reported to spread rapidly across the world and commonly identified in Escherichia coli and Klebsiella pneumoniae. To guide inhibitor design, we used diversely substituted 3-aryl and 3-heteroaryl benzoic acids to probe the active site of OXA-48 for useful enzyme-inhibitor interactions. In the presented study, a focused fragment library containing 49 3-substituted benzoic acid derivatives were synthesised and biochemically characterized. Based on crystallographic data from 33 fragment-enzyme complexes, the fragments could be classified into R or R binders by their overall binding conformation in relation to the binding of the R and R side groups of imipenem. Moreover, binding interactions attractive for future inhibitor design were found and their usefulness explored by the rational design and evaluation of merged inhibitors from orthogonally binding fragments. The best inhibitors among the resulting 3,5-disubstituted benzoic acids showed inhibitory potential in the low micromolar range (IC = 2.9 µM). For these inhibitors, the complex X-ray structures revealed non-covalent binding to Arg250, Arg214 and Tyr211 in the active site and the interactions observed with the mono-substituted fragments were also identified in the merged structures. [Mh] Termos MeSH primário: Desenho de Drogas Resistência Microbiana a Medicamentos/efeitos dos fármacos Bibliotecas de Moléculas Pequenas/farmacologia Inibidores de beta-Lactamases/farmacologia beta-Lactamases/metabolismo [Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga Escherichia coli/enzimologia Klebsiella pneumoniae/enzimologia Estrutura Molecular Bibliotecas de Moléculas Pequenas/síntese química Bibliotecas de Moléculas Pequenas/química Relação Estrutura-Atividade Inibidores de beta-Lactamases/síntese química Inibidores de beta-Lactamases/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Small Molecule Libraries); 0 (beta-Lactamase Inhibitors); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (oxacillinase) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180302 [Lr] Data última revisão: 180302 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180120 [St] Status: MEDLINE

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[PMID]: 28458485 [Au] Autor: Ibrahim Y; Sani Y; Saleh Q; Saleh A; Hakeem G [Ad] Endereço: Department of Microbiology, Faculty of Science, Bayero University, Kano, Nigeria. [Ti] Título: Phenotypic Detection of Extended Spectrum Beta lactamase and Carbapenemase Co-producing Clinical Isolates from Two Tertiary Hospitals in Kano, North West Nigeria. [So] Source: Ethiop J Health Sci;27(1):3-10, 2017 Jan. [Is] ISSN: 2413-7170 [Cp] País de publicação: Ethiopia [La] Idioma: eng [Ab] Resumo: BACKGROUND: Continue rise in unprofessional use of antibiotics in our hospitals and communities is worrisome. A research study was therefore conducted to detect extended spectrum beta-lactamase (ESBL), carbapenemase, metallobeta lactamase and their co-production phenotypically from isolates obtained from patients admitted to or attending two tertiary hospitals in Kano, Nigeria. METHOD: A total of 248 isolates of Escherichia coli and Klebsiella pneumoniaewere screened phenotypically for ESBL production and carbapenemase production according to CLS1 2012 breakpoints using double disk synergy test and modified Hodge test (MHT) respectively. Antibiotic susceptibility of the organisms was tested against colistin, tigecycline and 3 flouroquinolones. RESULTS: The result shows that 58.0% of the isolates were ESBL producers with higher percentage in K. pneumoniae (62.9%). Further, about 40.3% and 36.6% of the isolates were resistant to meropenem and imipenem respectively. However, E. coli showed higher resistance to meropenem (47.1%) while K. pneumoniae showed higher resistance to imipenem (44.4%). Co-productions of carbapenemase and ESBL were observed in both E. coli and K. pneumoniae. Carbapenemase producing isolates were more obtained from uro-pathogens and wound isolates, with almost all the cases of co-production of the ß lactamases occurring in urine and cathertips isolates. Overall susceptibilities of the isolates to colistin and tigecycline were 64.6and70.0% respectively, but isolates were less susceptible to flouroquinolones. CONCLUSION: The finding of the study therefore indicates that carbapenem resistance is mediated by carbapenemase production and or overproduction of ESBL coupled with reduced porins. Co-production of carbapenemase, MBLs and ESBLs by some of the isolates is worrisome. Susceptibility to colistin and tigecycline was still promising, but increasing resistance to flouroquinolones has been observed. [Mh] Termos MeSH primário: Proteínas de Bactérias/biossíntese Escherichia coli/isolamento & purificação Klebsiella pneumoniae/isolamento & purificação beta-Lactamases/biossíntese [Mh] Termos MeSH secundário: Antibacterianos/farmacologia Seres Humanos Testes de Sensibilidade Microbiana Nigéria Fenótipo Centros de Atenção Terciária [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180228 [Lr] Data última revisão: 180228 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170502 [St] Status: MEDLINE

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[PMID]: 29315344 [Au] Autor: Gong L; Huang YT; Wong CH; Chao WC; Wu ZY; Wei CL; Liu PY [Ad] Endereço: Genome Technologies, The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, United States of America. [Ti] Título: Culture-independent analysis of liver abscess using nanopore sequencing. [So] Source: PLoS One;13(1):e0190853, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The identification of microbial species has depended predominantly upon culture-based techniques. However, the difficulty with which types of organisms are cultured implies that the grown species may be overrepresented by both cultivation and plate counts. In recent years, culture-independent analysis using high-throughput sequencing has been advocated for use as a point-of-care diagnostic tool. Although it offers a rapid and unbiased survey to characterize the pathogens in clinical specimens, its accuracy is reduced by the high level of contamination of human DNA. In this paper, we propose using a culture-independent analysis for a Klebsiella pneumoniae clinical strain within a liver abscess using nanopore sequencing. Owing to the highly-contaminated cell population within a liver abscess, we managed to reduce the confounding effects of human DNA through the use of DNase and differential centrifugation. Genomic DNA was sequenced through the use of Nanopore MinION sequencer and analyzed using a suite of bioinformatics approaches. K. pneumoniae was successfully identified along with antibiotic-resistant genes. Our results indicate that, by integrating real-time nanopore sequencing and bioinformatics software, real-time pathogen identification in a liver abscess can be achieved. [Mh] Termos MeSH primário: Abscesso Hepático/microbiologia Nanoporos [Mh] Termos MeSH secundário: Sequenciamento de Nucleotídeos em Larga Escala Seres Humanos Klebsiella pneumoniae/efeitos dos fármacos Klebsiella pneumoniae/genética Klebsiella pneumoniae/isolamento & purificação Abscesso Hepático/diagnóstico por imagem Testes de Sensibilidade Microbiana [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180110 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0190853

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[PMID]: 29261254 [Au] Autor: Sundararaman B; Muthuramu KL [Ti] Título: A comparison of mango seed kernel powder, mango leaf powder and Manilkara zapota seed powder for decolorization of methylene blue dye and antimicrobial activity. [So] Source: J Environ Biol;37(6):1315-21, 2016 11. [Is] ISSN: 0254-8704 [Cp] País de publicação: India [La] Idioma: eng [Ab] Resumo: The waste mango seed generated from mango pulp industry in India is a major problem in handling the waste and hence, conversion of mango seed kernel. Mango seeds were collected and processed for oil extraction. Decolorization of methylene blue was achieved by mango seed kernel powder, mango leaf powder and Manilkara zapota seed powder. Higher efficiency was attained in mango seed kernel powder when compared to mango leaf powder and Manilkara zapota seed powder. A 60 to 95 % of removal efficiency was achieved by varying concentration. Effect of pH, dye concentration, adsorbent dosage and temperature were studied. Mango seed kernel powder is a better option that can be used as an adsorbent for the removal of methylene blue and basic red dye from its aqueous solutions. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Mangifera/química Manilkara/química Azul de Metileno/química Folhas de Planta/química Sementes/química [Mh] Termos MeSH secundário: Adsorção Antibacterianos/química Antifúngicos/farmacologia Concentração de Íons de Hidrogênio Klebsiella pneumoniae/efeitos dos fármacos Penicillium chrysogenum/efeitos dos fármacos Temperatura Ambiente Poluentes Químicos da Água/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Water Pollutants, Chemical); T42P99266K (Methylene Blue) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180219 [Lr] Data última revisão: 180219 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171221 [St] Status: MEDLINE

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[PMID]: 29248295 [Au] Autor: Zhang E; Wang MM; Huang SC; Xu SM; Cui DY; Bo YL; Bai PY; Hua YG; Xiao CL; Qin S [Ad] Endereço: School of Pharmaceutical Sciences, Institute of Drug Discovery and Development, Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou 450001, PR China; Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Pr [Ti] Título: NOTA analogue: A first dithiocarbamate inhibitor of metallo-ß-lactamases. [So] Source: Bioorg Med Chem Lett;28(2):214-221, 2018 01 15. [Is] ISSN: 1464-3405 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The emergence of antibiotic drug (like carbapenem) resistance is being a global crisis. Among those resistance factors of the ß-lactam antibiotics, the metallo-ß-lactamases (MBLs) is one of the most important reasons. In this paper, a series of cyclic dithiocarbamate compounds were synthesized and their inhibition activities against MBLs were initially tested combined with meropenem (MEM) by in vitro antibacterial efficacy tests. Sodium 1,4,7-triazonane-1,4,7-tris(carboxylodithioate) (compound 5) was identified as the most active molecule to restore the activity of MEM. Further anti-bacterial effectiveness assessment, compound 5 restored the activity of MEM against Escherichia coli, Citrobacter freundii, Proteus mirabilis and Klebsiella pneumonia, which carried resistance genes of bla . The compound 5 was non-hemolytic, even at a concentration of 1000 µg/mL. This compound was low toxic toward mammalian cells, which was confirmed by fluorescence microscopy image and the inhibition rate of HeLa cells. The Ki value of compounds 5 against NDM-1 MBL was 5.63 ±â€¯1.27 µM. Zinc ion sensitivity experiments showed that the inhibitory effect of compound 5 as a MBLs inhibitor was influenced by zinc ion. The results of the bactericidal kinetics displayed that compound 5 as an adjuvant assisted MEM to kill all bacteria. These data validated that this NOTA dithiocarbamate analogue is a good inhibitor of MBLs. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Compostos Heterocíclicos/farmacologia Inibidores de beta-Lactamases/farmacologia beta-Lactamases/metabolismo [Mh] Termos MeSH secundário: Antibacterianos/síntese química Antibacterianos/química Sobrevivência Celular/efeitos dos fármacos Citrobacter freundii/efeitos dos fármacos Relação Dose-Resposta a Droga Escherichia coli/efeitos dos fármacos Células HeLa Compostos Heterocíclicos/síntese química Compostos Heterocíclicos/química Seres Humanos Klebsiella pneumoniae/efeitos dos fármacos Testes de Sensibilidade Microbiana Estrutura Molecular Proteus mirabilis/efeitos dos fármacos Relação Estrutura-Atividade Inibidores de beta-Lactamases/síntese química Inibidores de beta-Lactamases/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Heterocyclic Compounds); 0 (beta-Lactamase Inhibitors); 56491-86-2 (1,4,7-triazacyclononane-N,N',N''-triacetic acid); EC 3.5.2.6 (beta-Lactamases) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171218 [St] Status: MEDLINE

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[PMID]: 27771726 [Au] Autor: Obi AT; Andraska E; Kanthi Y; Luke CE; Elfline M; Madathilparambil S; Siahaan TJ; Jaffer FA; Wakefield TW; Raghavendran K; Henke PK [Ad] Endereço: Conrad Jobst Vascular Research Laboratory, University of Michigan Medical School, Ann Arbor, Mich., USA. [Ti] Título: Gram-Negative Pneumonia Alters Large-Vein Cell-Adhesion Molecule Profile and Potentiates Experimental Stasis Venous Thrombosis. [So] Source: J Vasc Res;53(3-4):186-195, 2016. [Is] ISSN: 1423-0135 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: BACKGROUND/AIMS: Pneumonia is a significant risk factor for the development of venous thrombosis (VT). Cell-adhesion molecules (CAMs) are linked to the pathogenesis of both pneumonia and VT. We hypothesized that remote infection would confer a prothrombogenic milieu via systemic elevation of CAMs. METHODS: Lung injury was induced in wild-type (C57BL/6) mice by lung contusion or intratracheal inoculation with Klebsiella pneumoniae or saline controls. K. pneumoniae-treated mice and controls additionally underwent inferior vena cava (IVC) ligation to generate VT. RESULTS: Lung-contusion mice demonstrated no increase in E-selectin or P-selectin whereas mice infected with K. pneumoniae demonstrated increased circulating P-selectin, ICAM-1, VCAM-1 and thrombin-antithrombin (TAT) complexes. Mice with pneumonia formed VT 3 times larger than controls, demonstrated significantly more upregulation of vein-wall and systemic CAMs, and formed erythrocyte-rich thrombi. CONCLUSION: Elevated CAM expression was identified in mice with pneumonia, but not lung contusion, indicating that the type of inflammatory stimulus and the presence of infection drive the vein-wall response. Elevation of CAMs was associated with amplified VT and may represent an alternate mechanism by which to target the prevention of VT. [Mh] Termos MeSH primário: Moléculas de Adesão Celular/sangue Infecções por Klebsiella/complicações Klebsiella pneumoniae/patogenicidade Pneumonia Bacteriana/complicações Veia Cava Inferior/metabolismo Trombose Venosa/etiologia [Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/sangue Lesão Pulmonar Aguda/complicações Animais Antitrombina III Moléculas de Adesão Celular/antagonistas & inibidores Modelos Animais de Doenças Fibrinolíticos/farmacologia Molécula 1 de Adesão Intercelular/sangue Infecções por Klebsiella/sangue Infecções por Klebsiella/tratamento farmacológico Infecções por Klebsiella/microbiologia Ligadura Masculino Camundongos Endogâmicos C57BL Selectina-P/sangue Peptídeo Hidrolases/sangue Pneumonia Bacteriana/sangue Pneumonia Bacteriana/tratamento farmacológico Pneumonia Bacteriana/microbiologia Regulação para Cima Molécula 1 de Adesão de Célula Vascular/sangue Veia Cava Inferior/cirurgia Trombose Venosa/sangue Trombose Venosa/microbiologia Trombose Venosa/prevenção & controle [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cell Adhesion Molecules); 0 (Fibrinolytic Agents); 0 (Icam1 protein, mouse); 0 (P-Selectin); 0 (Vascular Cell Adhesion Molecule-1); 0 (antithrombin III-protease complex); 126547-89-5 (Intercellular Adhesion Molecule-1); 9000-94-6 (Antithrombin III); EC 3.4.- (Peptide Hydrolases) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161025 [St] Status: MEDLINE

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[PMID]: 29254478 [Au] Autor: Zhang H; Yang Q; Liao K; Ni Y; Yu Y; Hu B; Sun Z; Huang W; Wang Y; Wu A; Feng X; Luo Y; Chu Y; Chen S; Cao B; Su J; Duan Q; Zhang S; Shao H; Kong H; Gui B; Hu Z; Badal R; Xu Y [Ad] Endereço: Division of Microbiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Wangfujing Street, Beijing, 100730, China. [Ti] Título: Update of incidence and antimicrobial susceptibility trends of Escherichia coli and Klebsiella pneumoniae isolates from Chinese intra-abdominal infection patients. [So] Source: BMC Infect Dis;17(1):776, 2017 12 18. [Is] ISSN: 1471-2334 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: To evaluate in vitro susceptibilities of aerobic and facultative Gram-negative bacterial (GNB) isolates from intra-abdominal infections (IAIs) to 12 selected antimicrobials in Chinese hospitals from 2012 to 2014. METHODS: Hospital acquired (HA) and community acquired (CA) IAIs were collected from 21 centers in 16 Chinese cities. Extended spectrum beta-lactamase (ESBL) status and antimicrobial susceptibilities were determined at a central laboratory using CLSI broth microdilution and interpretive standards. RESULTS: From all isolated strains the Enterobacteriaceae (81.1%) Escherichia coli accounted for 45.4% and Klebsiella pneumoniae for 20.1%, followed by Enterobacter cloacae (5.2%), Proteus mirabilis (2.1%), Citrobacter freundii (1.8%), Enterobacter aerogenes (1.8%), Klebsiella oxytoca (1.4%), Morganella morganii (1.2%), Serratia marcescens (0.7%), Citrobacter koseri (0.3%), Proteus vulgaris (0.3%) and others (1.0%). Non- Enterobacteriaceae (18.9%) included Pseudomonas aeruginosa (9.8%), Acinetobacter baumannii (6.7%), Stenotrophomonas maltophilia (0.9%), Aeromonas hydrophila (0.4%) and others (1.1%). ESBL-screen positive Escherichia coli isolates (ESBL+) showed a decreasing trend from 67.5% in 2012 to 58.9% in 2014 of all Escherichia coli isolates and the percentage of ESBL+ Klebsiella pneumoniae isolates also decreased from 2012 through 2014 (40.4% to 26.6%), which was due to reduced percentages of ESBL+ isolates in HA IAIs for both bacteria. The overall susceptibilities of all 5160 IAI isolates were 87.53% to amikacin (AMK), 78.12% to piperacillin-tazobactam (TZP) 81.41% to imipenem (IMP) and 73.12% to ertapenem (ETP). The susceptibility of ESBL-screen positive Escherichia coli strains was 96.77%-98.8% to IPM, 91.26%-93.16% to ETP, 89.48%-92.75% to AMK and 84.86%-89.34% to TZP, while ESBL-screen positive Klebsiella pneumoniae strains were 70.56%-80.15% susceptible to ETP, 80.0%-87.5% to IPM, 83.82%-87.06% to AMK and 63.53%-68.38% to TZP within the three year study. Susceptibilities to all cephalosporins and fluoroquinolones were less than 50% beside 66.5% and 56.07% to cefoxitin (FOX) for ESBL+ Escherichia coli and Klebsiella pneumoniae strains respectively. CONCLUSIONS: The total ESBL+ rates decreased in Escherichia coli and Klebsiella pneumoniae IAI isolates due to fewer prevalence in HA infections. IPM, ETP and AMK were the most effective antimicrobials against ESBL+ Escherichia coli and Klebsiella pneumoniae IAI isolates in 2012-2014 and a change of fluoroquinolone regimens for Chinese IAIs is recommended. [Mh] Termos MeSH primário: Abdome/microbiologia Antibacterianos/farmacologia Infecções por Escherichia coli/microbiologia Escherichia coli/efeitos dos fármacos Infecções por Klebsiella/microbiologia Klebsiella pneumoniae/efeitos dos fármacos [Mh] Termos MeSH secundário: Cefalosporinas/farmacologia China/epidemiologia Infecções Comunitárias Adquiridas/epidemiologia Infecções Comunitárias Adquiridas/microbiologia Infecção Hospitalar/microbiologia Escherichia coli/classificação Escherichia coli/genética Escherichia coli/isolamento & purificação Infecções por Escherichia coli/epidemiologia Seres Humanos Imipenem/farmacologia Incidência Infecções Intra-Abdominais/microbiologia Infecções por Klebsiella/epidemiologia Klebsiella pneumoniae/classificação Klebsiella pneumoniae/genética Klebsiella pneumoniae/isolamento & purificação Testes de Sensibilidade Microbiana beta-Lactamas/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0 (beta-Lactams); 71OTZ9ZE0A (Imipenem); G32F6EID2H (ertapenem) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180214 [Lr] Data última revisão: 180214 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171220 [St] Status: MEDLINE [do] DOI: 10.1186/s12879-017-2873-z

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