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[PMID]:28235124
[Au] Autor:Hustedt JW; Ahmed S
[Ad] Endereço:Department of Orthopedics, Banner University Medical Center, Phoenix, AZ. joshua.hustedt@email.arizona.edu.
[Ti] Título:Plesiomonas shigelloides Periprosthetic Knee Infection After Consumption of Raw Oysters.
[So] Source:Am J Orthop (Belle Mead NJ);46(1):E32-E34, 2017 Jan/Feb.
[Is] ISSN:1934-3418
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. Common pathogens include Staphylococcus aureus, streptococcus, enterococcus, Escherichia coli, and Pseudomonas aeruginosa. However, there are many cases in which rare bacteria are isolated. This case report describes a periprosthetic knee infection caused by Plesiomonas shigelloides. In the United States, P shigelloides and 2 other Vibrionaceae family members, Vibrio vulnificus and Vibrio parahaemolyticus, are most often contracted from eating raw oysters and shellfish. P shigelloides usually causes a self-limiting watery diarrhea, but in immunosuppressed people it can cause septicemia. In this case report, a chemically and biologically immunosuppressed man consumed raw oysters and developed P shigelloides septicemia and acute periprosthetic knee infection that required surgical intervention.
[Mh] Termos MeSH primário: Infecções por Bactérias Gram-Negativas/etiologia
Ostreidae/microbiologia
Plesiomonas/isolamento & purificação
Infecções Relacionadas à Prótese/etiologia
Alimentos Marinhos/efeitos adversos
[Mh] Termos MeSH secundário: Idoso
Animais
Desbridamento
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/cirurgia
Seres Humanos
Masculino
Infecções Relacionadas à Prótese/microbiologia
Infecções Relacionadas à Prótese/cirurgia
Alimentos Marinhos/microbiologia
Irrigação Terapêutica
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE


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Sidrim, José Júlio Costa
Rocha, Marcos Fábio Gadelha
Texto completo
[PMID]:27885558
[Au] Autor:Castelo-Branco DS; Silva AL; Monteiro FO; Guedes GM; Sales JA; Oliveira JS; Maia Junior JE; Miranda SA; Sidrim JJ; Alencar LP; Brilhante RS; Cordeiro RA; Bandeira TJ; Pereira Neto WA; Rocha MF
[Ad] Endereço:Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Rua Coronel Nunes de Melo s/n, Rodolfo Teófilo, Fortaleza, Ceará, CEP 60430-270, Brazil.
[Ti] Título:Aeromonas and Plesiomonas species from scarlet ibis (Eudocimus ruber) and their environment: monitoring antimicrobial susceptibility and virulence.
[So] Source:Antonie Van Leeuwenhoek;110(1):33-43, 2017 Jan.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study aimed at evaluating the role of captive scarlet ibises (Eudocimus ruber) and their environment as reservoirs of Aeromonas spp. and Plesiomonas spp., and analyzing the in vitro antimicrobial susceptibility and virulence of the recovered bacterial isolates. Thus, non-lactose and weak-lactose fermenting, oxidase positive Gram-negative bacilli were recovered from cloacal samples (n = 30) of scarlet ibises kept in a conservational facility and from water samples (n = 30) from their environment. Then, the antimicrobial susceptibility, hemolytic activity and biofilm production of the recovered Aeromonas spp. and Plesiomonas shigelloides strains were assessed. In addition, the virulence-associated genes of Aeromonas spp. were detected. Ten Aeromonas veronii bv. sobria, 2 Aeromonas hydrophila complex and 10 P. shigelloides were recovered. Intermediate susceptibility to piperacillin-tazobactam and cefepime was observed in 2 Aeromonas spp. and 1 P. shigelloides, respectively, and resistance to gentamicin was observed in 4 P. shigelloides. The automated susceptibility analysis revealed resistance to piperacillin-tazobactam and meropenem among Aeromonas spp. and intermediate susceptibility to gentamicin among P. shigelloides. All Aeromonas isolates presented hemolytic activity, while 3 P. shigelloides were non-hemolytic. All Aeromonas spp. and 3/10 P. shigelloides were biofilm-producers, at 28 °C, while 10 Aeromonas spp. and 6/10 P. shigelloides produced biofilms, at 37 °C. The most prevalent virulence genes of Aeromonas spp. were asa1 and ascV. Scarlet ibises and their environment harbour potentially pathogenic bacteria, thus requiring monitoring and measures to prevent contamination of humans and other animals.
[Mh] Termos MeSH primário: Aeromonas/isolamento & purificação
Doenças das Aves/microbiologia
Aves/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Plesiomonas/isolamento & purificação
[Mh] Termos MeSH secundário: Aeromonas/classificação
Aeromonas/efeitos dos fármacos
Aeromonas/patogenicidade
Animais
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Ecossistema
Infecções por Bactérias Gram-Negativas/microbiologia
Plesiomonas/classificação
Plesiomonas/efeitos dos fármacos
Plesiomonas/patogenicidade
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-016-0771-9


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[PMID]:27878230
[Au] Autor:Meng S; Wang Y; Wang Y; Ye C
[Ad] Endereço:State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China.
[Ti] Título:Rapid and sensitive detection of Plesiomonas shigelloides by cross­priming amplification of the hugA gene.
[So] Source:Mol Med Rep;14(6):5443-5450, 2016 Dec.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Plesiomonas shigelloides (P. shigelloides) is implicated as an aetiological agent of human gastroenteritis in humans, for which reliable laboratory detection of P. shigelloides is clinically and epidemiologically desirable. A simple molecular method for rapid detection of P. shigelloides using cross­priming amplification (CPA) has been developed, with hugA as the target. The hugA gene is required for haem iron utilisation and is critical for the survival and growth of P. shigelloides. The assay output was visualised as a colour change with no need to open the reaction tubes, and no false­positive results were detected for the 33 non­ P. shigelloides strains examined to assess assay specificity. The limit of detection was 200 fg P. shigelloides DNA per reaction and 3x103 CFU per g in human stools, which was 100 and 10­fold more sensitive than polymerase chain reaction, respectively. The CPA method was used to detect the presence of P. shigelloides in stool specimens from 70 patients with diarrhoea and 30 environmental water samples, with no difference in accuracy between the CPA assay and the biological culture. The present study, therefore, suggests that the P. shigelloides hugA CPA assay may represent a valuable tool for rapid and sensitive detection of P. shigelloides in primary care facilities and clinical laboratories.
[Mh] Termos MeSH primário: Genes Bacterianos
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/microbiologia
Técnicas de Amplificação de Ácido Nucleico
Plesiomonas/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Diarreia/diagnóstico
Diarreia/microbiologia
Fezes/microbiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5937


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[PMID]:27494421
[Au] Autor:Kaszowska M; Stojkovic K; Niedziela T; Lugowski C
[Ad] Endereço:Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114, Wroclaw, Poland. Electronic address: marta.kaszowska@iitd.pan.wroc.pl.
[Ti] Título:The O-antigen of Plesiomonas shigelloides serotype O36 containing pseudaminic acid.
[So] Source:Carbohydr Res;434:1-5, 2016 Nov 03.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by H and C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: [Formula: see text] These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the ß-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs.2013, 11 (2), 440-454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434-10447).
[Mh] Termos MeSH primário: Antígenos O/química
Plesiomonas/genética
[Mh] Termos MeSH secundário: Sequência de Carboidratos
Espectroscopia de Ressonância Magnética
Antígenos O/genética
Plesiomonas/química
Plesiomonas/imunologia
Plesiomonas/metabolismo
Sorogrupo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (O Antigens)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE


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[PMID]:27039769
[Au] Autor:Rattanata N; Klaynongsruang S; Daduang S; Tavichakorntrakool R; Limpaiboon T; Lekphrom R; Boonsiri P; Daduang J
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand E-mail : jurpoo@kku.ac.th.
[Ti] Título:Inhibitory Effects of Gallic Acid Isolated from Caesalpinia mimosoides Lamk on Cholangiocarcinoma Cell Lines and Foodborne Pathogenic Bacteria.
[So] Source:Asian Pac J Cancer Prev;17(3):1341-5, 2016.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:Gallic acid was isolated from Caesalpinia mimosoides Lamk and the structure s identified based on spectroscopic analysis and comparison with authentic compound. In this study we compared the ability of natural gallic acid (nGA) and commercial gallic acid (cGA) to inhibit the proliferation of cholangiocarcinoma cell lines (M213, M214) and foodborne pathogenic bacteria (Salmonella spp. and Plesiomonas shigelloides). Both nGA and cGA had the same inhibitory effects on cell proliferation by inducing apoptosis of cholangiocarcinoma cell lines. In addition, nGA inhibited growth of foodborne pathogenic bacteria in the same manner as cGA. Our results suggest that nGA from Caesalpinia mimosoides Lamk is a potential anticancer and antibacterial compound. However, in vivo studies are needed to elucidate the specific mechanisms involved.
[Mh] Termos MeSH primário: Neoplasias dos Ductos Biliares/tratamento farmacológico
Ductos Biliares Intra-Hepáticos/efeitos dos fármacos
Caesalpinia/química
Colangiocarcinoma/tratamento farmacológico
Doenças Transmitidas por Alimentos/tratamento farmacológico
Ácido Gálico/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Antineoplásicos/farmacologia
Apoptose/efeitos dos fármacos
Neoplasias dos Ductos Biliares/patologia
Ductos Biliares Intra-Hepáticos/patologia
Proliferação Celular/efeitos dos fármacos
Colangiocarcinoma/patologia
Microbiologia de Alimentos
Doenças Transmitidas por Alimentos/patologia
Ácido Gálico/isolamento & purificação
Seres Humanos
Extratos Vegetais/isolamento & purificação
Plesiomonas/efeitos dos fármacos
Salmonella/efeitos dos fármacos
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents); 0 (Plant Extracts); 632XD903SP (Gallic Acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160405
[St] Status:MEDLINE


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[PMID]:26960939
[Au] Autor:Janda JM; Abbott SL; McIver CJ
[Ad] Endereço:Kern County Public Health Laboratory, Department of Public Health Services, Bakersfield, California, USA jandam@co.kern.ca.us.
[Ti] Título:Plesiomonas shigelloides Revisited.
[So] Source:Clin Microbiol Rev;29(2):349-74, 2016 Apr.
[Is] ISSN:1098-6618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:After many years in the family Vibrionaceae, the genus Plesiomonas, represented by a single species, P. shigelloides, currently resides in the family Enterobacteriaceae, although its most appropriate phylogenetic position may yet to be determined. Common environmental reservoirs for plesiomonads include freshwater ecosystems and estuaries and inhabitants of these aquatic environs. Long suspected as being an etiologic agent of bacterial gastroenteritis, convincing evidence supporting this conclusion has accumulated over the past 2 decades in the form of a series of foodborne outbreaks solely or partially attributable to P. shigelloides. The prevalence of P. shigelloides enteritis varies considerably, with higher rates reported from Southeast Asia and Africa and lower numbers from North America and Europe. Reasons for these differences may include hygiene conditions, dietary habits, regional occupations, or other unknown factors. Other human illnesses caused by P. shigelloides include septicemia and central nervous system disease, eye infections, and a variety of miscellaneous ailments. For years, recognizable virulence factors potentially associated with P. shigelloides pathogenicity were lacking; however, several good candidates now have been reported, including a cytotoxic hemolysin, iron acquisition systems, and lipopolysaccharide. While P. shigelloides is easy to identify biochemically, it is often overlooked in stool samples due to its smaller colony size or relatively low prevalence in gastrointestinal samples. However, one FDA-approved PCR-based culture-independent diagnostic test system to detect multiple enteropathogens (FilmArray) includes P. shigelloides on its panel. Plesiomonads produce ß-lactamases but are typically susceptible to many first-line antimicrobial agents, including quinolones and carbapenems.
[Mh] Termos MeSH primário: Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/epidemiologia
Plesiomonas/classificação
[Mh] Termos MeSH secundário: Doenças Transmitidas por Alimentos/epidemiologia
Doenças Transmitidas por Alimentos/microbiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Seres Humanos
Técnicas de Diagnóstico Molecular
Filogenia
Plesiomonas/genética
Plesiomonas/isolamento & purificação
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160311
[St] Status:MEDLINE
[do] DOI:10.1128/CMR.00103-15


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[PMID]:26852764
[Au] Autor:Patel S; Gandhi D; Mehta V; Bhatia K; Epelbaum O
[Ti] Título:Plesiomonas shigelloides : an extremely rare cause of Spontaneous Bacterial Peritonitis.
[So] Source:Acta Gastroenterol Belg;79(1):52-3, 2016 Mar.
[Is] ISSN:1784-3227
[Cp] País de publicação:Belgium
[La] Idioma:eng
[Ab] Resumo:Plesiomonas shigelloides, a rare cause of gastroenteritis in humans, is a gram negative rod belonging to the Enterobacteriaceae family. In addition, it has also been implicated in extra-intestinal infection, but prevalence data on such infections have been limited to case reports. To date there has been only one published case of P. shigelloides causing spontaneous bacterial peritonitis (SBP). We describe another patient with P. shigelloides SBP and compare our findings with those from the original case.
[Mh] Termos MeSH primário: Líquido Ascítico/microbiologia
Peritonite/microbiologia
Plesiomonas/isolamento & purificação
[Mh] Termos MeSH secundário: Idoso
Seres Humanos
Cirrose Hepática Alcoólica/complicações
Masculino
Peritonite/complicações
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160923
[Lr] Data última revisão:
160923
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


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[PMID]:26806178
[Au] Autor:Bowman JK; Zhang XC; Hack JB
[Ad] Endereço:Warren Alpert Medical School of Brown University, Providence, RI. Electronic address: Jason_Bowman@Brown.edu.
[Ti] Título:Plesiomonas shigelloides meningitis in an adult in the ED.
[So] Source:Am J Emerg Med;34(7):1329.e1-2, 2016 Jul.
[Is] ISSN:1532-8171
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções por Bactérias Gram-Negativas/diagnóstico
Meningites Bacterianas/diagnóstico
Plesiomonas/isolamento & purificação
[Mh] Termos MeSH secundário: Serviço Hospitalar de Emergência
Seres Humanos
Masculino
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160126
[St] Status:MEDLINE


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[PMID]:26056883
[Au] Autor:Kim KT; Lee SH; Kwak D
[Ti] Título:PREVALENCE, BIOCHEMICAL CHARACTERISTICS, AND ANTIBIOTIC SUSCEPTIBILITY OF AEROMONADS, VIBRIOS, AND PLESIOMONADS ISOLATED FROM DIFFERENT SOURCES AT A ZOO.
[So] Source:J Zoo Wildl Med;46(2):298-305, 2015 Jun.
[Is] ISSN:1042-7260
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aeromonas spp., Vibrio parahaemolyticus , and Plesiomonas shigelloides are commonly implicated in foodborne and waterborne diarrheal illnesses of humans and other animals. The present study assessed the prevalence, biochemical characteristics, and antibiotic susceptibility of Aeromonas spp., V. parahaemolyticus , and P. shigelloides by analyzing samples from 729 sources at a zoo, including animal feces (n=607), watering facilities (n=104), and pond water samples (n=18). Of the 729 samples collected, 40 (5.5%) contained one of these four species of bacteria: A. hydrophila (n=16; 2.2%), A. sobria (n=12; 1.6%), V. parahaemolyticus (n=10; 1.4%), and P. shigelloides (n=2; 0.3%). The 16 isolates of A. hydrophila came from three fecal samples, eight watering facilities, and five pond water samples. The 12 isolates of A. sobria came from four fecal samples, three watering facilities, and five pond water samples. The 10 isolates of V. parahaemolyticus came from one fecal sample and nine watering facilities. The two isolates of P. shigelloides came from one watering facility and one pond water sample. Of the 40 isolates, 16 (40.0%), 21 (52.5%), and three (7.5%) originated from mammals, birds, and reptiles, respectively. All isolates tested positive for NO3, tryptophan, p-nitrophenyl-ß-D-galactopyranoside, glucose assimilation, N-acetyl-glucosamine, maltose, gluconate, malate, and oxidase. Aeromonas spp. and V. parahaemolyticus exhibited similar biochemical characteristics, whereas P. shigelloides exhibited distinct fermentation characteristics. All the isolated strains exhibited hemolytic activity; variable results of DNase, protease, and Congo red uptake tests; and resistance to ampicillin, bacitracin, novobiocin, penicillin, and vancomycin. All the strains were sensitive to amikacin, chloramphenicol, colistin, gentamicin, kanamycin, norfloxacin, and trimethoprim-sulfadimethoxazole. Because of the high proportion of asymptomatic carriers of these potentially pathogenic bacteria and their wide distribution, consistent monitoring of food and water sources is necessary to prevent disease outbreaks.
[Mh] Termos MeSH primário: Aeromonas/isolamento & purificação
Animais de Zoológico
Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Plesiomonas/isolamento & purificação
Vibrio/isolamento & purificação
[Mh] Termos MeSH secundário: Aeromonas/classificação
Aeromonas/efeitos dos fármacos
Aeromonas/patogenicidade
Animais
Abrigo para Animais
Plesiomonas/classificação
Plesiomonas/efeitos dos fármacos
Plesiomonas/patogenicidade
Vibrio/classificação
Vibrio/efeitos dos fármacos
Vibrio/patogenicidade
Virulência
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:150610
[Lr] Data última revisão:
150610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150610
[St] Status:MEDLINE
[do] DOI:10.1638/2014-0194R.1


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[PMID]:25958688
[Au] Autor:Liu Z; Ke X; Lu M; Gao F; Cao J; Zhu H; Wang M
[Ti] Título:[Identification and pathological observation of a pathogenic Plesiomonas shigelloides strain isolated from cultured tilapia (Oreochromis niloticus)].
[So] Source:Wei Sheng Wu Xue Bao;55(1):96-106, 2015 Jan 04.
[Is] ISSN:0001-6209
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: A mass mortality of tilapia broke out in an aquaculture farm in Panyu, Guangdong Province in May, 2013. Affected fish showed blackening of body color, haemorrhageing on surface, scales shedding, fin rotting, and the presence of yellow liver, dark red spleen, enlarged gallbladder and ascitic fluid in the abdominal cavity. The purpose of this research was isolating and identifying the pathogen. METHODS: We isolated a suspicious bacteria strain PYS1 from diseased fish with significant pathological signs. The homology of 16S rRNA gene sequence of strain PYS1 and its morphological, cultural, and physical and chemical characteristics were studied for its identification. Its pathogenicity was investigated by recursive infection experiment and histopathological study. Its effective medicines was screened by antibiotic sensitive test. RESULTS: The results showed that strain PYS1 was Plesiomonas shigelloides clustered with P. shigelloides strains isolated from other fishes in the molecular phylogenetic tree of 16S rRNA gene sequences. Strain PYS1 was multiple drug resistant and only sensitive to a small part of 31 tested antibiotics (e.g., ceftriaxone, cefaclor, cefazolin, etc.). The symptoms of tilapia (O. niloticus) artificially infected with strain PYS1 were similar with natural infected fish. The half lethal dose (LD50) of strain PYS1 to tilapia was 1.425 x 10(8) CFU per fish. Paraffin sections showed intestine, liver, spleen, kidney and heart tissue injury caused by the strain. CONCLUSION: Our study demonstrated that P. shigelloides was the pathogen of cultured tilapia in the aquaculture farm and first reported its bacterial pathogenicity on Nile tilapia.
[Mh] Termos MeSH primário: Ciclídeos/microbiologia
Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Plesiomonas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Ciclídeos/crescimento & desenvolvimento
Doenças dos Peixes/patologia
Pesqueiros
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/patologia
Testes de Sensibilidade Microbiana
Filogenia
Plesiomonas/classificação
Plesiomonas/efeitos dos fármacos
Plesiomonas/genética
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150511
[Lr] Data última revisão:
150511
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150512
[St] Status:MEDLINE



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