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Pesquisa : B03.440.450.425.600.800 [Categoria DeCS]
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[PMID]:28936060
[Au] Autor:Al-Kharashi AS; Al-Kharshi AS; Al-Faky YH
[Ad] Endereço:Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:Endophthalmitis Due to after Pars Plana Vitrectomy with Devastating Outcome.
[So] Source:Middle East Afr J Ophthalmol;24(2):116-118, 2017 Apr-Jun.
[Is] ISSN:0975-1599
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Postoperative infectious endophthalmitis is rare, yet devastating loss of vision or loss of the eye can occur due to a highly purulent microorganism or uncontrolled endophthalmitis that may spread to all coats of the eye. We report, herewith, a case of rapidly progressive postoperative endophthalmitis after pars plana vitrectomy which ended up with enucleation. The isolated microorganism was which has not been reported as causative bacteria of postoperative infections following pars plana vitrectomy.
[Mh] Termos MeSH primário: Endoftalmite/microbiologia
Enucleação Ocular/métodos
Infecções por Proteus/microbiologia
Proteus vulgaris/isolamento & purificação
Infecção da Ferida Cirúrgica/microbiologia
Vitrectomia/efeitos adversos
[Mh] Termos MeSH secundário: Idoso
Endoftalmite/diagnóstico
Endoftalmite/cirurgia
Feminino
Seres Humanos
Infecções por Proteus/diagnóstico
Infecções por Proteus/cirurgia
Descolamento Retiniano/cirurgia
Infecção da Ferida Cirúrgica/diagnóstico
Infecção da Ferida Cirúrgica/cirurgia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_134_15


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[PMID]:28634338
[Au] Autor:Singh LR; Avula SR; Raj S; Srivastava A; Palnati GR; Tripathi CKM; Pasupuleti M; Sashidhara KV
[Ad] Endereço:Division of Medicinal and Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, India.
[Ti] Título:Coumarin-benzimidazole hybrids as a potent antimicrobial agent: synthesis and biological elevation.
[So] Source:J Antibiot (Tokyo);70(9):954-961, 2017 Aug.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Molecular hybridization approach is an emerging tool in drug discovery for designing new pharmacophores with biological activity. A novel, new series of coumarin-benzimidazole hybrids were designed, synthesized and evaluated for their broad spectrum antimicrobial activity. Among all the synthesized molecules, compound (E)-3-(2-1H-benzo[d]imidazol-1-yl)-1-((4-chlorobenzyl)oxy)imino)ethyl)-2H-chromen-2-one showed the most promising broad spectrum antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Proteus vulgaris. In addition, it has showed no cytotoxicity and hemolysis at 10 times the MIC concentration. SAR studies indicate that position of the chlorine atom in the hybrid critically determines the antibacterial activity.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Anti-Infecciosos/farmacologia
Benzimidazóis/farmacologia
Cumarínicos/farmacologia
Desenho de Drogas
Farmacorresistência Bacteriana Múltipla
Modelos Moleculares
[Mh] Termos MeSH secundário: Animais
Antibacterianos/efeitos adversos
Antibacterianos/síntese química
Antibacterianos/química
Anti-Infecciosos/efeitos adversos
Anti-Infecciosos/síntese química
Anti-Infecciosos/química
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Benzimidazóis/efeitos adversos
Benzimidazóis/síntese química
Benzimidazóis/química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Células do Tecido Conjuntivo/citologia
Células do Tecido Conjuntivo/efeitos dos fármacos
Cumarínicos/efeitos adversos
Cumarínicos/síntese química
Cumarínicos/química
Hemólise/efeitos dos fármacos
Seres Humanos
Hidrocarbonetos Clorados/efeitos adversos
Hidrocarbonetos Clorados/síntese química
Hidrocarbonetos Clorados/química
Hidrocarbonetos Clorados/farmacologia
Camundongos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Proteus vulgaris/efeitos dos fármacos
Proteus vulgaris/crescimento & desenvolvimento
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/crescimento & desenvolvimento
Estereoisomerismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((E)-3-(2-1H-benzo(d)imidazol-1-yl)-1-((4-chlorobenzyl)oxy)imino)ethyl)-2H-chromen-2-one); 0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Benzimidazoles); 0 (Coumarins); 0 (Hydrocarbons, Chlorinated)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.70


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[PMID]:28498641
[Au] Autor:Wang CH; Xie XR; Liu WS; Hou GG; Sun JF; Zhao F; Cong W; Li HJ; Xin WY
[Ad] Endereço:School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai, China.
[Ti] Título:Quaternary ammonium salts substituted by 5-phenyl-1,3,4-oxadiazole-2-thiol as novel antibacterial agents with low cytotoxicity.
[So] Source:Chem Biol Drug Des;90(5):943-952, 2017 Nov.
[Is] ISSN:1747-0285
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Twenty-one novel 5-phenyl-1,3,4-oxadiazole-2-thiol (POT) substituted N-hydroxyethyl quaternary ammonium salts (6a-g, 7a-g, 8a-g) were prepared and characterized by FTIR, NMR, and elemental analysis. Compounds 6a, 6c, and 8a were confirmed by X-ray single-crystal diffraction. They display the unsurpassed antibacterial activity against Staphylococcus aureus, α-H-tococcus, Escherichia coli, P. aeruginosa, Proteus vulgaris, Canidia Albicans, especially 6g, 7g, 8g with dodecyl group. Compounds 8a-d with N,N-dihydroxyethyl and POT groups display unsurpassed antibacterial activity and non-toxicity. The structure-activity relationships indicate that POT and flexible dihydroxyethyl group in QAS are necessary for antibacterial activity and cytotoxicity. SEM and TEM images of E. coli morphologies of 8d show the antibacterial agents can adhere to membrane surfaces to inhibit bacterial growth by disrupting peptidoglycan formation and releasing bacterial cytoplasm from cell membranes.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Oxidiazóis/farmacologia
Compostos de Amônio Quaternário/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Infecções Bacterianas/tratamento farmacológico
Escherichia coli/efeitos dos fármacos
Seres Humanos
Testes de Sensibilidade Microbiana
Modelos Moleculares
Oxidiazóis/química
Proteus vulgaris/efeitos dos fármacos
Compostos de Amônio Quaternário/química
Staphylococcus aureus/efeitos dos fármacos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-phenyl-1,3,4-oxadiazole-2-thiol); 0 (Anti-Bacterial Agents); 0 (Oxadiazoles); 0 (Quaternary Ammonium Compounds)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1111/cbdd.13020


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[PMID]:27789329
[Au] Autor:Daneshjou S; Dabirmanesh B; Rahimi F; Khajeh K
[Ad] Endereço:Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
[Ti] Título:Porous silicon nanoparticle as a stabilizing support for chondroitinase.
[So] Source:Int J Biol Macromol;94(Pt B):852-858, 2017 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chondroitinase ABCI (cABCI) from Proteus vulgaris is a drug enzyme that can be used to treat spinal cord injuries. One of the main problems of chondroitinase ABC1 is its low thermal stability. The objective of the current study was to stabilize the enzyme through entrapment within porous silicon (pSi) nanoparticles. pSi was prepared by an electrochemical etch of p-type silicon using hydrofluoric acid/ethanol. The size of nanoparticles were determined 180nm by dynamic light scattering and the mean pore diameter was in the range of 40-60nm obtained by scanning electron microscopy. Enzymes were immobilized on porouse silicon nanoparticles by entrapment. The capacity of matrix was 35µg enzyme per 1mg of silicon. The immobilized enzyme displayed lower V values compared to the free enzyme, but Km values were the same for both enzymes. Immobilization significantly increased the enzyme stability at various temperatures (-20, 4, 25 and 37°C). For example, at 4°C, the free enzyme (in 10mM imidazole) retained 20% of its activity after 100min, while the immobilized one retained 50% of its initial activity. Nanoparticles loading capacity and the enzyme release rate showed that the selected particles could be a pharmaceutically acceptable carrier for chondroitinase.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Condroitina ABC Liase/química
Enzimas Imobilizadas/química
Nanopartículas/química
Silício/química
[Mh] Termos MeSH secundário: Sulfatos de Condroitina/química
Liberação Controlada de Fármacos
Estabilidade Enzimática
Etanol/química
Ácido Fluorídrico/química
Cinética
Tamanho da Partícula
Porosidade
Proteus vulgaris/química
Proteus vulgaris/enzimologia
Proteínas Recombinantes/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Enzymes, Immobilized); 0 (Recombinant Proteins); 3K9958V90M (Ethanol); 9007-28-7 (Chondroitin Sulfates); EC 4.2.2.20 (Chondroitin ABC Lyase); RGL5YE86CZ (Hydrofluoric Acid); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


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[PMID]:27159722
[Au] Autor:Hemalatha A; Mohammed Esa SAR; Suresh M; Thajuddin N; Anantharaman P
[Ad] Endereço:a Department of Microbiology , Bharathidasan University , Tiruchirappalli , Tamilnadu , India.
[Ti] Título:Identification of Odontella aurita by rbcL gene sequence - a high antibacterial potential centric marine diatom.
[So] Source:Mitochondrial DNA A DNA Mapp Seq Anal;28(5):655-661, 2017 Sep.
[Is] ISSN:2470-1408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antibacterial potential of centric marine diatoms has been compared against the clinical pathogens and identified the potential diatom by rbcL gene sequencing. Totally, five diatoms namely Odontella aurita, Thalassiosira subtilis, Chaetoceros curvisetus, Skeletonema costatum and Coscinodiscus centralis were isolated from Cuddalore coastal waters. The diatoms were morphologically identified and isolated using micro capillary-pipette and serial dilution method. The isolated diatoms were cultured in Guillard's f/2 medium to get biomass for the antibacterial study. The dried biomass of the cultured diatoms was individually extracted with methanol, ethanol and hexane. All the obtained extracts were tested against Staphylococcus haemolyticus, Proteus vulgarius and Vibrio alginolyticus. The crude ethanol extract of O. aurita was exhibited highest zone of inhibition against all the test pathogens. The MIC of O. aurita was recorded as 50 µg/ml against both Staphylococcus haemolyticus and Proteus vulgarius whilst 75 µg/ml against Vibrio alginolyticus. This study indicates that O. aurita possesses antibacterial activities but the release of antibiotics depends on physical or chemical rupture of algal cells and extractive solvents. Based on the maximum antibacterial activity, O. aurita was further identified by rbcL gene sequencing. The rbcL gene could be an identical region for the species level identification of diatoms.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Diatomáceas/crescimento & desenvolvimento
Etanol/farmacologia
Ribulose-Bifosfato Carboxilase/genética
Água/parasitologia
[Mh] Termos MeSH secundário: Antibacterianos/metabolismo
Diatomáceas/genética
Diatomáceas/isolamento & purificação
Etanol/metabolismo
Testes de Sensibilidade Microbiana
Filogenia
Fitoplâncton/genética
Fitoplâncton/crescimento & desenvolvimento
Fitoplâncton/isolamento & purificação
Proteus vulgaris/efeitos dos fármacos
Análise de Sequência de DNA
Staphylococcus haemolyticus/efeitos dos fármacos
Vibrio alginolyticus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 059QF0KO0R (Water); 3K9958V90M (Ethanol); EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160510
[St] Status:MEDLINE
[do] DOI:10.3109/24701394.2016.1166222


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[PMID]:27501924
[Au] Autor:Li Y; Chen Z; Zhou Z; Yuan Q
[Ad] Endereço:Department of Biotechnology, Beijing Polytechnic, No.1 Taiyanggong Shaoyaoju, Chao Yang District, Beijing 100029, China. Electronic address: liyeyashi@163.com.
[Ti] Título:Expression, purification and characterization of GAPDH-ChSase ABC I from Proteus vulgaris in Escherichia coli.
[So] Source:Protein Expr Purif;128:36-41, 2016 Dec.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chondroitinases (ChSases) are a family of polysaccharide lyases that can depolymerize high molecular weight chondroitin sulfate (CS) and dermatan sulfate (DS). In this study, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is stably expressed in different cells like normal cells and cancer cells and the expression is relatively insensitive to experimental conditions, was expressed as a fusion protein with ChSase ABC I. Results showed that the expression level and enzyme activity of GAPDH-ChSase ABC I were about 2.2 and 3.0 times higher than those of ChSase ABC I. By optimization of fermentation conditions, higher productivity of ChSase ABC I was achieved as 880 ± 61 IU/g wet cell weight compared with the reported ones. The optimal temperature and pH of GAPDH-ChSase ABC I were 40 °C and 7.5, respectively. GAPDH-ChSase ABC I had a kcat/Km of 131 ± 4.1 L/µmol s and the catalytic efficiency was decreased as compared to ChSase ABC I. The relative activity of GAPDH-ChSase ABC I remained 89% after being incubated at 30 °C for 180 min and the thermostability of ChSase ABC I was enhanced by GAPDH when it was incubated at 30, 35, 40 and 45 °C.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Condroitina ABC Liase
Escherichia coli/metabolismo
Expressão Gênica
Gliceraldeído-3-Fosfato Desidrogenases
Proteus vulgaris/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/biossíntese
Proteínas de Bactérias/genética
Proteínas de Bactérias/isolamento & purificação
Catálise
Condroitina ABC Liase/biossíntese
Condroitina ABC Liase/química
Condroitina ABC Liase/genética
Condroitina ABC Liase/isolamento & purificação
Estabilidade Enzimática
Escherichia coli/genética
Gliceraldeído-3-Fosfato Desidrogenases/biossíntese
Gliceraldeído-3-Fosfato Desidrogenases/química
Gliceraldeído-3-Fosfato Desidrogenases/genética
Gliceraldeído-3-Fosfato Desidrogenases/isolamento & purificação
Temperatura Alta
Proteus vulgaris/enzimologia
Proteínas Recombinantes de Fusão/biossíntese
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Fusion Proteins); EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases); EC 4.2.2.20 (Chondroitin ABC Lyase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160810
[St] Status:MEDLINE


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[PMID]:27423610
[Au] Autor:Carwardine D; Wong LF; Fawcett JW; Muir EM; Granger N
[Ad] Endereço:University of Bristol, School of Veterinary Sciences, Regenerative Medicine Laboratory, Biomedical Science Building, University Walk, Bristol BS8 1TD, United Kingdom. Electronic address: darren.carwardine@bristol.ac.uk.
[Ti] Título:Canine olfactory ensheathing cells from the olfactory mucosa can be engineered to produce active chondroitinase ABC.
[So] Source:J Neurol Sci;367:311-8, 2016 Aug 15.
[Is] ISSN:1878-5883
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A multitude of factors must be overcome following spinal cord injury (SCI) in order to achieve clinical improvement in patients. It is thought that by combining promising therapies these diverse factors could be combatted with the aim of producing an overall improvement in function. Chondroitin sulphate proteoglycans (CSPGs) present in the glial scar that forms following SCI present a significant block to axon regeneration. Digestion of CSPGs by chondroitinase ABC (ChABC) leads to axon regeneration, neuronal plasticity and functional improvement in preclinical models of SCI. However, the enzyme activity decays at body temperature within 24-72h, limiting the translational potential of ChABC as a therapy. Olfactory ensheathing cells (OECs) have shown huge promise as a cell transplant therapy in SCI. Their beneficial effects have been demonstrated in multiple small animal SCI models as well as in naturally occurring SCI in canine patients. In the present study, we have genetically modified canine OECs from the mucosa to constitutively produce enzymatically active ChABC. We have developed a lentiviral vector that can deliver a mammalian modified version of the ChABC gene to mammalian cells, including OECs. Enzyme production was quantified using the Morgan-Elson assay that detects the breakdown products of CSPG digestion in cell supernatants. We confirmed our findings by immunolabelling cell supernatant samples using Western blotting. OECs normal cell function was unaffected by genetic modification as demonstrated by normal microscopic morphology and the presence of the low affinity neurotrophin receptor (p75(NGF)) following viral transduction. We have developed the means to allow production of active ChABC in combination with a promising cell transplant therapy for SCI repair.
[Mh] Termos MeSH primário: Condroitina ABC Liase/metabolismo
Mucosa Olfatória/citologia
Mucosa Olfatória/enzimologia
Transdução Genética/métodos
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Western Blotting
Condroitina ABC Liase/genética
Proteoglicanas de Sulfatos de Condroitina/metabolismo
Cães
Vetores Genéticos
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Células HEK293
Células HeLa
Seres Humanos
Imuno-Histoquímica
Lentivirus/genética
Mucosa Olfatória/transplante
Proteus vulgaris/enzimologia
Proteus vulgaris/genética
Receptores de Fator de Crescimento Neural/metabolismo
Traumatismos da Medula Espinal/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Chondroitin Sulfate Proteoglycans); 0 (Receptors, Nerve Growth Factor); 147336-22-9 (Green Fluorescent Proteins); EC 4.2.2.20 (Chondroitin ABC Lyase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160718
[St] Status:MEDLINE


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[PMID]:27332132
[Au] Autor:Chang PS; McLane LT; Fogg R; Scrimgeour J; Temenoff JS; Granqvist A; Curtis JE
[Ad] Endereço:School of Physics, Georgia Institute of Technology, Atlanta, Georgia; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia.
[Ti] Título:Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans.
[So] Source:Biophys J;110(12):2739-2750, 2016 Jun 21.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The hyaluronan-rich pericellular matrix (PCM) plays physical and chemical roles in biological processes ranging from brain plasticity, to adhesion-dependent phenomena such as cell migration, to the onset of cancer. This study investigates how the spatial distribution of the large negatively charged bottlebrush proteoglycan, aggrecan, impacts PCM morphology and cell surface access. The highly localized pericellular milieu limits transport of nanoparticles in a size-dependent fashion and sequesters positively charged molecules on the highly sulfated side chains of aggrecan. Both rat chondrocyte and human mesenchymal stem cell PCMs possess many unused binding sites for aggrecan, showing a 2.5x increase in PCM thickness from ∼7 to ∼18 µm when provided exogenous aggrecan. Yet, full extension of the PCM occurs well below aggrecan saturation. Hence, cells equipped with hyaluronan-rich PCM can in principle manipulate surface accessibility or sequestration of molecules by tuning the bottlebrush proteoglycan content to alter PCM porosity and the number of electrostatic binding sites.
[Mh] Termos MeSH primário: Agrecanas/metabolismo
Matriz Extracelular/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/metabolismo
Membrana Celular/metabolismo
Células Cultivadas
Condroitina ABC Liase/metabolismo
Difusão
Matriz Extracelular/microbiologia
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Seres Humanos
Ácido Hialurônico/metabolismo
Células Mesenquimais Estromais/metabolismo
Nanopartículas/metabolismo
Pinças Ópticas
Porosidade
Proteus vulgaris
Ratos
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aggrecans); 0 (Bacterial Proteins); 147336-22-9 (Green Fluorescent Proteins); 9004-61-9 (Hyaluronic Acid); EC 4.2.2.20 (Chondroitin ABC Lyase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160623
[St] Status:MEDLINE


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[PMID]:27311501
[Au] Autor:Nazari-Robati M; Golestani A; Asadikaram G
[Ad] Endereço:Department of Clinical Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: mnazari@kmu.ac.ir.
[Ti] Título:Improvement of proteolytic and oxidative stability of Chondroitinase ABC I by cosolvents.
[So] Source:Int J Biol Macromol;91:812-7, 2016 Oct.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Recently, utilization of the enzyme Chondroitinase ABC I (cABC I) has received considerable attention in treatment of spinal cord injury. cABC I removes chondroitin sulfate proteoglycans which are inhibitory to axon growth and enhances nerve regeneration. Therefore, determination of cABC I resistance to proteolysis and oxidation provides valuable information for optimizing its clinical application. In this work, proteolytic stability of cABC I to trypsin and chymotrypsin as well as its oxidative resistance to H2O2 was measured. Moreover, the effect of cosolvents glycerol, sorbitol and trehalose on cABC I proteolytic and oxidative stability was determined. The results indicated that cABC I is highly susceptible to proteolysis and oxidation. Comparison of proteolytic patterns demonstrated a high degree of similarity which confirmed the exposure of specific regions of cABC I to proteolysis. However, proteolytic degradation was significantly reduced in the presence of cosolvents. In addition, cosolvents decreased the rate of both cABC I proteolytic and oxidative inactivation. Notably, the degree of stabilization provided by these cosolvents varied greatly. These findings indicated the high potential of cosolvents in protein stabilization to proteolysis and oxidative inactivation.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Condroitina ABC Liase/química
Glicerol/química
Proteólise
Proteus vulgaris/enzimologia
Sorbitol/química
Trealose/química
[Mh] Termos MeSH secundário: Quimotripsina/química
Estabilidade Enzimática
Oxirredução
Solventes/química
Tripsina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Solvents); 506T60A25R (Sorbitol); B8WCK70T7I (Trehalose); EC 3.4.21.1 (Chymotrypsin); EC 3.4.21.4 (Trypsin); EC 4.2.2.20 (Chondroitin ABC Lyase); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE


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[PMID]:27074858
[Au] Autor:Priyanka KP; Sukirtha TH; Balakrishna KM; Varghese T
[Ad] Endereço:Department of Studies in Physics, Mangalore University, Mangalagangotri 574 199, Karnataka, India.
[Ti] Título:Microbicidal activity of TiO2 nanoparticles synthesised by sol-gel method.
[So] Source:IET Nanobiotechnol;10(2):81-6, 2016 Apr.
[Is] ISSN:1751-8741
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, the authors investigated antimicrobial activity of TiO2 nanoparticles (NPs) synthesised by sol-gel method. As synthesised TiO2 NPs were characterised by X-ray diffraction, scanning electron microscopy and ultraviolet-visible absorption spectroscopy. The antimicrobial activity of calcined TiO2 nanoparticle samples was examined in day light on Gram positive bacteria (Staphylococcus aureus, Streptococcus pneumonia and Bacillus subtilis), Gram negative bacteria (Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungal test pathogen Candida albicans. The synthesised TiO2 NPs were found to be effective in visible light against Streptococcus pneumonia, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa and Candida albicans.
[Mh] Termos MeSH primário: Antibacterianos/síntese química
Nanopartículas Metálicas/química
Nanotecnologia/métodos
Transição de Fase
Titânio/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Escherichia coli/efeitos dos fármacos
Escherichia coli/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Varredura
Proteus vulgaris/efeitos dos fármacos
Proteus vulgaris/crescimento & desenvolvimento
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/crescimento & desenvolvimento
Streptococcus pneumoniae/efeitos dos fármacos
Streptococcus pneumoniae/crescimento & desenvolvimento
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 15FIX9V2JP (titanium dioxide); D1JT611TNE (Titanium)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160415
[St] Status:MEDLINE
[do] DOI:10.1049/iet-nbt.2015.0038



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