Base de dados : MEDLINE
Pesquisa : B03.440.450.425.814 [Categoria DeCS]
Referências encontradas : 1232 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 124 ir para página                         

  1 / 1232 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29362365
[Au] Autor:Zhang Y; Kastman EK; Guasto JS; Wolfe BE
[Ad] Endereço:Department of Biology, Tufts University, 200 Boston Avenue, Medford, MA, 02155, USA.
[Ti] Título:Fungal networks shape dynamics of bacterial dispersal and community assembly in cheese rind microbiomes.
[So] Source:Nat Commun;9(1):336, 2018 01 23.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Most studies of bacterial motility have examined small-scale (micrometer-centimeter) cell dispersal in monocultures. However, bacteria live in multispecies communities, where interactions with other microbes may inhibit or facilitate dispersal. Here, we demonstrate that motile bacteria in cheese rind microbiomes use physical networks created by filamentous fungi for dispersal, and that these interactions can shape microbial community structure. Serratia proteamaculans and other motile cheese rind bacteria disperse on fungal networks by swimming in the liquid layers formed on fungal hyphae. RNA-sequencing, transposon mutagenesis, and comparative genomics identify potential genetic mechanisms, including flagella-mediated motility, that control bacterial dispersal on hyphae. By manipulating fungal networks in experimental communities, we demonstrate that fungal-mediated bacterial dispersal can shift cheese rind microbiome composition by promoting the growth of motile over non-motile community members. Our single-cell to whole-community systems approach highlights the interactive dynamics of bacterial motility in multispecies microbiomes.
[Mh] Termos MeSH primário: Queijo/microbiologia
DNA Bacteriano/genética
Fungos/crescimento & desenvolvimento
Hifas/crescimento & desenvolvimento
Interações Microbianas/genética
Microbiota/genética
Serratia/genética
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Actinobacteria/genética
Actinobacteria/crescimento & desenvolvimento
Elementos de DNA Transponíveis
Firmicutes/classificação
Firmicutes/genética
Firmicutes/crescimento & desenvolvimento
Flagelos/genética
Flagelos/ultraestrutura
Fungos/ultraestrutura
Sequenciamento de Nucleotídeos em Larga Escala
Hifas/ultraestrutura
Movimento/fisiologia
Mucor/crescimento & desenvolvimento
Mucor/ultraestrutura
Mutação
Penicillium/crescimento & desenvolvimento
Penicillium/ultraestrutura
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/crescimento & desenvolvimento
Serratia/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02522-z


  2 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29023528
[Au] Autor:Asem D; Leo VV; Passari AK; Tonsing MV; Joshi JB; Uthandi S; Hashem A; Abd Allah EF; Singh BP
[Ad] Endereço:Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Aizawl, Mizoram University, Mizoram, India.
[Ti] Título:Evaluation of gastrointestinal bacterial population for the production of holocellulose enzymes for biomass deconstruction.
[So] Source:PLoS One;12(10):e0186355, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gastrointestinal (GI) habitat of ruminant and non-ruminant animals sustains a vast ensemble of microbes that are capable of utilizing lignocellulosic plant biomass. In this study, an indigenous swine (Zovawk) and a domesticated goat (Black Bengal) were investigated to isolate bacteria having plant biomass degrading enzymes. After screening and enzymatic quantification of eighty-one obtained bacterial isolates, Serratia rubidaea strain DBT4 and Aneurinibacillus aneurinilyticus strain DBT87 were revealed as the most potent strains, showing both cellulase and xylanase production. A biomass utilization study showed that submerged fermentation (SmF) of D2 (alkaline pretreated pulpy biomass) using strain DBT4 resulted in the most efficient biomass deconstruction with maximum xylanase (11.98 U/mL) and FPase (0.5 U/mL) activities (55°C, pH 8). The present study demonstrated that bacterial strains residing in the gastrointestinal region of non-ruminant swine are a promising source for lignocellulose degrading microorganisms that could be used for biomass conversion.
[Mh] Termos MeSH primário: Bacillales/enzimologia
Celulase/metabolismo
Trato Gastrointestinal/microbiologia
Serratia/enzimologia
[Mh] Termos MeSH secundário: Animais
Bacillales/classificação
Bacillales/genética
Bacillales/isolamento & purificação
Biomassa
Endo-1,4-beta-Xilanases/metabolismo
Cabras
Concentração de Íons de Hidrogênio
Cinética
Lignina/química
Lignina/metabolismo
Microscopia Eletrônica de Varredura
Filogenia
RNA Ribossômico 16S/classificação
RNA Ribossômico 16S/metabolismo
Serratia/classificação
Serratia/genética
Serratia/isolamento & purificação
Espectroscopia de Infravermelho com Transformada de Fourier
Suínos
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); EC 3.2.1.4 (Cellulase); EC 3.2.1.8 (Endo-1,4-beta Xylanases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186355


  3 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29020016
[Au] Autor:Kim H; Cheang UK; Kim MJ
[Ad] Endereço:Department of Mechanical Engineering, Southern Methodist University, Dallas, TX, United Stated of America.
[Ti] Título:Autonomous dynamic obstacle avoidance for bacteria-powered microrobots (BPMs) with modified vector field histogram.
[So] Source:PLoS One;12(10):e0185744, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to broaden the use of microrobots in practical fields, autonomous control algorithms such as obstacle avoidance must be further developed. However, most previous studies of microrobots used manual motion control to navigate past tight spaces and obstacles while very few studies demonstrated the use of autonomous motion. In this paper, we demonstrated a dynamic obstacle avoidance algorithm for bacteria-powered microrobots (BPMs) using electric field in fluidic environments. A BPM consists of an artificial body, which is made of SU-8, and a high dense layer of harnessed bacteria. BPMs can be controlled using externally applied electric fields due to the electrokinetic property of bacteria. For developing dynamic obstacle avoidance for BPMs, a kinematic model of BPMs was utilized to prevent collision and a finite element model was used to characteristic the deformation of an electric field near the obstacle walls. In order to avoid fast moving obstacles, we modified our previously static obstacle avoidance approach using a modified vector field histogram (VFH) method. To validate the advanced algorithm in experiments, magnetically controlled moving obstacles were used to intercept the BPMs as the BPMs move from the initial position to final position. The algorithm was able to successfully guide the BPMs to reach their respective goal positions while avoiding the dynamic obstacles.
[Mh] Termos MeSH primário: Algoritmos
Fontes de Energia Bioelétrica
Robótica/métodos
Serratia/fisiologia
[Mh] Termos MeSH secundário: Simulação por Computador
Movimento (Física)
Processos Estocásticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185744


  4 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28853686
[Au] Autor:Zhang CW; Zhang J; Zhao JJ; Zhao X; Zhao DF; Yin HQ; Zhang XX
[Ad] Endereço:1​Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
[Ti] Título:Serratia oryzae sp. nov., isolated from rice stems.
[So] Source:Int J Syst Evol Microbiol;67(8):2928-2933, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel endophytic bacterium, strain J11-6T, was isolated from rice stems. Its taxonomic position was investigated using a polyphasic approach. The novel strain was Gram-staining-negative, facultatively anaerobic, motile and rod-shaped. Although the results of phylogenetic analysis based on 16S rRNA gene sequences indicated that J11-6T represented a member of the genus Rahnella, multilocus sequence analysis (MLSA) on the basis of concatenated partial atpD, gyrB, rpoB and infB gene sequences showed a clear distinction of J11-6T from the type strains of species of the genus Rahnella but indicated that it lay within the clade of the genus Serratia. The phylogenetically closest species were Serratia fonticola and Serratia aquatilis on the basis of the results of the MLSA phylogenetic analysis. The predominant cellular fatty acids were C16 : 1ω7c (38.7 %) and C16 : 0 (25.0 %). The DNA G+C content was 53.2 mol%. The DNA-DNA relatedness was 17.4 % between J11-6T and Rahnella aquatilis CIP 78.65T, and 29.2 % between J11-6T and S. fonticola LMG 7882T which indicates that this strain represents a novel species of the genus Serratia. Characterization by genotypic and phenotypic analysis indicated that J11-6T (=ACCC 19934T=KCTC 52529T) represents a novel species of the genus Serratia, for which the name Serratia oryzae sp. nov. is proposed.
[Mh] Termos MeSH primário: Oryza/microbiologia
Filogenia
Caules de Planta/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Tipagem de Sequências Multilocus
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Serratia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002049


  5 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28784817
[Au] Autor:Petersen LM; LaCourse K; Schöner TA; Bode H; Tisa LS
[Ad] Endereço:Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, New Hampshire, USA.
[Ti] Título:Inactivation of the Major Hemolysin Gene Influences Expression of the Nonribosomal Peptide Synthetase Gene in the Insect Pathogen Serratia sp. Strain SCBI.
[So] Source:J Bacteriol;199(21), 2017 Nov 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hemolysins are important virulence factors for many bacterial pathogens, including The role of the major hemolysin gene in the insect pathogen sp. strain SCBI was investigated using both forward and reverse-genetics approaches. Introduction of the major hemolysin gene into resulted in a gain of both virulence and hemolytic activity. Inactivation of this hemolysin in sp. SCBI resulted in a loss of hemolysis but did not attenuate insecticidal activity. Unexpectedly, inactivation of the hemolysin gene in sp. SCBI resulted in significantly increased motility and increased antimicrobial activity. Reverse transcription-quantitative PCR (qRT-PCR) analysis of mutants with a disrupted hemolysin gene showed a dramatic increase in mRNA levels of a nonribosomal peptide synthetase gene, , which produces the surfactant serrawettin W2. Mutation of the gene in sp. SCBI resulted in highly varied antibiotic activity, motility, virulence, and hemolysis phenotypes that were dependent on the site of disruption within this 17.75-kb gene. When introduced into , increases rates of motility and confers antimicrobial activity. While it is unclear how inactivation of the major hemolysin gene influences the expression of , these results suggest that plays an important role in motility and antimicrobial activity in sp. SCBI. The opportunistic Gram-negative bacteria of the genus are widespread in the environment and can cause human illness. A comparative genomics analysis between and a new species from South Africa, termed sp. strain SCBI, shows that these two organisms are closely related but differ in pathogenesis. kills nematodes, while sp. SCBI is not harmful and forms a beneficial association with them. This distinction presented the opportunity to investigate potential differences in regulation of common virulence mechanisms between these two species. With the emergence of antibiotic-resistant microorganisms, there is a widespread need to understand the regulation of pathogenesis. The significance of this study is the presentation of evidence for cross-pathway regulation of virulence factors and how the elimination of one mechanism may be compensated for by the upregulation of others.
[Mh] Termos MeSH primário: Regulação Bacteriana da Expressão Gênica
Inativação Gênica
Proteínas Hemolisinas/genética
Proteínas Hemolisinas/metabolismo
Peptídeo Sintases/biossíntese
Serratia/genética
Serratia/metabolismo
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/metabolismo
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/metabolismo
Expressão Gênica
Perfilação da Expressão Gênica
Insetos/microbiologia
Insetos/fisiologia
Locomoção
Peptídeo Sintases/genética
RNA Mensageiro/análise
Reação em Cadeia da Polimerase em Tempo Real
Serratia/enzimologia
Serratia/patogenicidade
Análise de Sobrevida
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Hemolysin Proteins); 0 (RNA, Messenger); EC 6.3.2.- (Peptide Synthases); EC 6.3.2.- (non-ribosomal peptide synthase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE


  6 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28738515
[Au] Autor:Kumar M; Morya R; Gnansounou E; Larroche C; Thakur IS
[Ad] Endereço:School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
[Ti] Título:Characterization of carbon dioxide concentrating chemolithotrophic bacterium Serratia sp. ISTD04 for production of biodiesel.
[So] Source:Bioresour Technol;243:893-897, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Proteomics and metabolomics analysis has become a powerful tool for characterization of microbial ability for fixation of Carbon dioxide. Bacterial community of palaeoproterozoic metasediments was enriched in the shake flask culture in the presence of NaHCO . One of the isolate showed resistance to NaHCO (100mM) and was identified as Serratia sp. ISTD04 by 16S rRNA sequence analysis. Carbon dioxide fixing ability of the bacterium was established by carbonic anhydrase enzyme assay along with proteomic analysis by LC-MS/MS. In proteomic analysis 96 proteins were identified out of these 6 protein involved in carbon dioxide fixation, 11 in fatty acid metabolism, indicating the carbon dioxide fixing potency of bacterium along with production of biofuel. GC-MS analysis revealed that hydrocarbons and FAMEs produced by bacteria within the range of C -C and C -C respectively. Presence of 59% saturated and 41% unsaturated organic compounds, make it a better fuel composition.
[Mh] Termos MeSH primário: Biocombustíveis
Dióxido de Carbono
Serratia
[Mh] Termos MeSH secundário: Proteômica
RNA Ribossômico 16S
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (RNA, Ribosomal, 16S); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  7 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28695855
[Au] Autor:Tichy EM; Hardwick SW; Luisi BF; Salmond GPC
[Ad] Endereço:Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, England.
[Ti] Título:1.8 Šresolution crystal structure of the carbapenem intrinsic resistance protein CarF.
[So] Source:Acta Crystallogr D Struct Biol;73(Pt 7):549-556, 2017 Jul 01.
[Is] ISSN:2059-7983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The natural production of the ß-lactam antibiotic carbapenem in bacteria involves a group of enzymes that form a synthetic pathway as well as proteins that protect the cell from self-intoxification by the products. Here, the crystal structure of CarF, one of the two proteins that confer resistance to synthesis of the antibiotic in the host organism, is reported. The CarF fold places it within a widely occurring structural family, indicating an ancient structural origin from which the resistance function has been derived.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Carbapenêmicos/metabolismo
Serratia/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cristalografia por Raios X
Seres Humanos
Modelos Moleculares
Conformação Proteica
Serratia/metabolismo
Infecções por Serratia/microbiologia
Resistência beta-Lactâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carbapenems)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1107/S2059798317002236


  8 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28554571
[Au] Autor:Mikhailova AG; Rakitina TV; Timofeev VI; Karlinsky DM; Korzhenevskiy DA; Agapova YК; Vlaskina AV; Ovchinnikova MV; Gorlenko VA; Rumsh LD
[Ad] Endereço:Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St.16/10, Moscow, 117997, Russian Federation. Electronic address: anna.mikhailova@ibch.ru.
[Ti] Título:Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine.
[So] Source:Biochimie;139:125-136, 2017 Aug.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Oligopeptidase B (OpdB; EC 3.4.21.83) is a trypsin-like peptidase belonging to the family of serine prolyl oligopeptidases; two-domain structure of the enzyme includes C-terminal peptidase catalytic domain and N-terminal seven-bladed ß-propeller domain. Importance of the interface between these domains and particularly of the 5 salt bridges for enzyme activity was established for protozoan OpdBs. However, these salt bridges are not conserved in γ -proteobacterial OpdBs including the peptidase from Serratia proteamaculans (PSP). In this work, using comparative modelling and protozoan OpdBs' crystal structures we created 3D models of PSP in open and closed forms to elucidate the mechanism underlying inactivation of the truncated form of PSP1-655 obtained earlier. Analysis of the models shows that in the closed form of PSP charged amino acid residues of histidine loop, surrounding the catalytic triad His652, participate in formation of the inter-domain contact interface between catalytic and ß-propeller domains, while in the open form of PSP disconnection of the catalytic triad and distortion of these contacts can be observed. Complete destruction of this interface by site-directed mutagenesis causes inactivation of PSP while elimination of the individual contacts leads to differential effects on the enzyme activity and substrate specificity. Thus, we identified structural factors regulating activity of PSP and supposedly of other γ-proteobacterial OpdBs and discovered the possibility of directed modulation of their enzymatic features.
[Mh] Termos MeSH primário: Histidina/química
Mutação/genética
Serina Endopeptidases/metabolismo
Serratia/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Catálise
Domínio Catalítico
Histidina/genética
Hidrólise
Modelos Moleculares
Mutagênese Sítio-Dirigida
Homologia de Sequência de Aminoácidos
Serina Endopeptidases/química
Serina Endopeptidases/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4QD397987E (Histidine); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.83 (oligopeptidase B)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE


  9 / 1232 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28385486
[Au] Autor:Gupta A; Kumar M; Thakur IS
[Ad] Endereço:School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
[Ti] Título:Analysis and optimization of process parameters for production of polyhydroxyalkanoates along with wastewater treatment by Serratia sp. ISTVKR1.
[So] Source:Bioresour Technol;242:55-59, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A previously reported biodegrading bacterial strain Serratia sp. ISTVKR1 was studied for polyhydroxyalkanoate (PHA) production along with wastewater contaminant removal. Nile red fluorescence, GC-MS, FT-IR, NMR and TEM confirmed the accumulation of homopolymer poly-3-hydroxyvalerate (PHV) within the bacterial cells. Analysis of culture after 72h of bacterial treatment showed maximum COD removal (8.4-fold), non-detection of organic contaminants such as 1H-Cyclopropa [a] naphthalene (R.T.=10.12) using GC-MS and increased proportion of elements like Cr, Mn, Fe, Ni, Cu, Cd and Pb in the bacterial cell pellets by SEM-EDX analysis. Optimization of process parameters for enhanced PHA production along with wastewater treatment done using Response Surface Methodology (RSM) showed 5% and 0.74% increase in the PHA production (0.3368±0.13gL ) and % COD reduction (88.93±2.41) of wastewater, respectively. The study, thus established the production of PHA along with wastewater contaminant removal by Serratia sp. ISTVKR1.
[Mh] Termos MeSH primário: Poli-Hidroxialcanoatos
Serratia
Águas Residuais
[Mh] Termos MeSH secundário: Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polyhydroxyalkanoates); 0 (Waste Water)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE


  10 / 1232 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28335605
[Au] Autor:Motley JL; Stamps BW; Mitchell CA; Thompson AT; Cross J; You J; Powell DR; Stevenson BS; Cichewicz RH
[Ti] Título:Opportunistic Sampling of Roadkill as an Entry Point to Accessing Natural Products Assembled by Bacteria Associated with Non-anthropoidal Mammalian Microbiomes.
[So] Source:J Nat Prod;80(3):598-608, 2017 Mar 24.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Few secondary metabolites have been reported from mammalian microbiome bacteria despite the large numbers of diverse taxa that inhabit warm-blooded higher vertebrates. As a means to investigate natural products from these microorganisms, an opportunistic sampling protocol was developed, which focused on exploring bacteria isolated from roadkill mammals. This initiative was made possible through the establishment of a newly created discovery pipeline, which couples laser ablation electrospray ionization mass spectrometry (LAESIMS) with bioassay testing, to target biologically active metabolites from microbiome-associated bacteria. To illustrate this process, this report focuses on samples obtained from the ear of a roadkill opossum (Dideiphis virginiana) as the source of two bacterial isolates (Pseudomonas sp. and Serratia sp.) that produced several new and known cyclic lipodepsipeptides (viscosin and serrawettins, respectively). These natural products inhibited biofilm formation by the human pathogenic yeast Candida albicans at concentrations well below those required to inhibit yeast viability. Phylogenetic analysis of 16S rRNA gene sequence libraries revealed the presence of diverse microbial communities associated with different sites throughout the opossum carcass. A putative biosynthetic pathway responsible for the production of the new serrawettin analogues was identified by sequencing the genome of the Serratia sp. isolate. This study provides a functional roadmap to carrying out the systematic investigation of the genomic, microbiological, and chemical parameters related to the production of natural products made by bacteria associated with non-anthropoidal mammalian microbiomes. Discoveries emerging from these studies are anticipated to provide a working framework for efforts aimed at augmenting microbiomes to deliver beneficial natural products to a host.
[Mh] Termos MeSH primário: Produtos Biológicos/química
Lipoproteínas/química
Microbiota
Peptídeos Cíclicos/química
Pseudomonas/química
RNA Ribossômico 16S/genética
Serratia/química
[Mh] Termos MeSH secundário: Animais
Animais Selvagens
Variação Genética
Seres Humanos
Mamíferos
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Filogenia
Espectrometria de Massas por Ionização por Electrospray
Vertebrados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Lipoproteins); 0 (Peptides, Cyclic); 0 (RNA, Ribosomal, 16S); 140909-80-4 (serrawettin W2)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00772



página 1 de 124 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde