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[PMID]:28459604
[Au] Autor:Mickol RL; Page JL; Schuerger AC
[Ad] Endereço:1 Center for Space and Planetary Sciences, University of Arkansas , Fayetteville, Arkansas.
[Ti] Título:Magnesium Sulfate Salt Solutions and Ices Fail to Protect Serratia liquefaciens from the Biocidal Effects of UV Irradiation under Martian Conditions.
[So] Source:Astrobiology;17(5):401-412, 2017 May.
[Is] ISSN:1557-8070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The growth of Serratia liquefaciens has been demonstrated under martian conditions of 0.7 kPa (7 mbar), 0°C, and CO -enriched anoxic atmospheres (Schuerger et al., 2013, Astrobiology 13:115-131), but studies into the survivability of cells under hypersaline conditions that are likely to be encountered on Mars are lacking. Serratia liquefaciens cells were suspended in aqueous MgSO solutions, or frozen brines, and exposed to terrestrial (i.e., 101.3 kPa, 24°C, O /N -normal atmosphere) or martian (i.e., 0.7 kPa, -25°C, CO -anoxic atmosphere) conditions to assess the roles of MgSO and UV irradiation on the survival of S. liquefaciens. Four solutions were tested for their capability to attenuate martian UV irradiation in both liquid and frozen forms: sterile deionized water (SDIW), 10 mM PO buffer, 5% MgSO , and 10% MgSO . None of the solutions in either liquid or frozen forms provided enhanced protection against martian UV irradiation. Sixty minutes of UV irradiation reduced cell densities from 2.0 × 10 cells/mL to less than 10 cells/mL for both liquid and frozen solutions. In contrast, 3-4 mm of a Mars analog soil were sufficient to attenuate 100% of UV irradiation. Results suggest that terrestrial microorganisms may not survive on Sun-exposed surfaces on Mars, even if the cells are embedded in frozen martian brines composed of MgSO . However, if dispersed microorganisms can be covered by only a few millimeters of dust or regolith, long-term survival is probable. Key Words: Hypobaria-Mars-Planetary protection-Brines. Astrobiology 17, 401-412.
[Mh] Termos MeSH primário: Serratia liquefaciens/efeitos da radiação
Raios Ultravioleta
[Mh] Termos MeSH secundário: Exobiologia
Meio Ambiente Extraterreno
Gelo
Sulfato de Magnésio
Serratia liquefaciens/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ice); 7487-88-9 (Magnesium Sulfate)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171214
[Lr] Data última revisão:
171214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1089/ast.2015.1448


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[PMID]:28525654
[Au] Autor:Rahman MM; Yoon KB; Lim SJ; Jeon MG; Kim HJ; Kim HY; Cho JY; Chae HM; Park YC
[Ad] Endereço:Division of Forest Science, Kangwon National University, Chuncheon, Republic of Korea.
[Ti] Título:Molecular detection by analysis of the 16S rRNA gene of fecal coliform bacteria from the two Korean Apodemus species (Apodemus agrarius and A. peninsulae).
[So] Source:Genet Mol Res;16(2), 2017 May 18.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. To assess the microbial communities, especially fecal coliform bacteria, we used two methods. First, we isolated bacterial colonies onto the common media LB (lactose broth) agar, TSA (tryptic soy agar), and MRS (de Man, Rogosa, and Sharpe) agar, and then randomly select colonies from each plate and stocked them to the mother plate for genomic DNA isolation. Second, we analyzed bacterial colonies using the 16S rRNA gene molecular diagnostic method. Based on bacterial cultures and bacterial 16S rRNA gene markers, we detected four different bacterial species (Bacillus amyloliquefaciens, Escherichia coli, Staphylococcus xylosus, and Serratia liquefaciens) from fecal coliforms of the striped field mouse Apodemus agrarius and A. peninsulae in agricultural areas in South Korea. These results could help us to better understand the pathogen reservoirs of mice and initiate some preventive measures to mitigate the microbial risks associated with mouse fecal matter in agricultural production areas.
[Mh] Termos MeSH primário: Microbiota
Murinae/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillus amyloliquefaciens/genética
Bacillus amyloliquefaciens/isolamento & purificação
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Fezes/microbiologia
RNA Ribossômico 16S/genética
República da Coreia
Serratia liquefaciens/genética
Serratia liquefaciens/isolamento & purificação
Staphylococcus/genética
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029510


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[PMID]:28372152
[Au] Autor:Baglinière F; Tanguy G; Salgado RL; Jardin J; Rousseau F; Robert B; Harel-Oger M; Vanetti MC; Gaucheron F
[Ad] Endereço:Department of Microbiology, Universidade Federal de Viçosa, Viçosa, MG 36.570-900, Brazil; CAPES Foundation, Ministry of Education of Brazil, Brasília DF 70.040-020, Brazil.
[Ti] Título:Ser2 from Serratia liquefaciens L53: A new heat stable protease able to destabilize UHT milk during its storage.
[So] Source:Food Chem;229:104-110, 2017 Aug 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The heat-stable protease Ser2 is secreted by the species Serratia liquefaciens, a psychrotrophic bacteria frequently found in raw milk. To understand the physicochemical modifications of casein micelles induced by Ser2 and to confirm its implication in UHT milk destabilization, the enzyme was purified and added to microfiltered raw milk before UHT treatment. UHT milk destabilization was investigated during 90days of storage. A visual destabilization appeared after 8days of storage with the presence of sediment. Zeta potential increase and formation of aggregates were observed during the storage. Using tandem mass spectrometry, numerous released peptides from the four caseins were identified at the end of storage. Caseins were hydrolyzed in the preferential order ß->α ->κ->α . No specific peptidic hydrolysed bond was detected. The present study confirmed that the presence of the protease Ser2 in raw milk can be one of the main causes of UHT milk destabilization.
[Mh] Termos MeSH primário: Armazenamento de Alimentos/métodos
Leite/química
Peptídeo Hidrolases/química
Serratia liquefaciens/química
[Mh] Termos MeSH secundário: Animais
Temperatura Alta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28222216
[Au] Autor:Baglinière F; Salgado RL; Salgado CA; Vanetti MC
[Ad] Endereço:Dept. of Microbiology, Federal Univ. of Viçosa, Viçosa, MG, 36.570-900, Brazil.
[Ti] Título:Biochemical Characterization of an Extracellular Heat-Stable Protease from Serratia liquefaciens Isolated from Raw Milk.
[So] Source:J Food Sci;82(4):952-959, 2017 Apr.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The protease Ser2 secreted by the psychrotrophic strain Serratia liquefaciens L53, a highly proteolytic strain isolated from Brazilian raw milk was purified and characterized. Using azocasein as substrate, Ser2 exhibited activity in a wide range of pH (5 to 10) and temperature (4 to 60 °C). The optimal activity was detected at pH 8.0 and at a temperature of 37 °C. This protease, still active at 4, 7, and 10 °C, was strongly inhibited by chelating agents and by dithiothreitol, a reducing agent. These results confirmed that Ser2 belongs to the peptidase family M10 and requires Ca , Zn , and disulfide bridges for stability. This protease is able to hydrolyze three kinds of casein in the preferential order of κ→ ß→ α-casein. Highly heat-stable in skimmed, semi-skimmed, and whole milk at 140°C with D-values of 2.8, 3.9, and 4.5 min, respectively, Ser2 showed a residual activity between 87 and 100 percent after heat-treatment of 65 °C for 30 min, 72 °C for 20 s, and 140 °C for 4 s that are commonly used in dairy industries. As the protease AprX that is mainly secreted by Pseudomonas genus, Ser2 could be one of the main causes of UHT milk destabilization during storage.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Endopeptidases/metabolismo
Temperatura Alta
Leite/microbiologia
Serratia liquefaciens/enzimologia
[Mh] Termos MeSH secundário: Animais
Brasil
Caseínas/química
Caseínas/metabolismo
Clonagem Molecular
Eletroforese em Gel de Poliacrilamida
Contaminação de Alimentos
Microbiologia de Alimentos
Armazenamento de Alimentos
Concentração de Íons de Hidrogênio
Proteólise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Caseins); 0 (azocasein); EC 3.4.- (Endopeptidases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13660


  5 / 52 MEDLINE  
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[PMID]:28099826
[Au] Autor:Schlapbach C; Sendi P
[Ad] Endereço:Bern University Hospital, Bern, Switzerland christoph.schlapbach@insel.ch.
[Ti] Título:Serrated Marine Nose.
[So] Source:N Engl J Med;376(3):267, 2017 Jan 19.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções por Micobactéria não Tuberculosa/patologia
Mycobacterium marinum/isolamento & purificação
Doenças Nasais/patologia
Nariz/patologia
Infecções por Serratia/patologia
Serratia liquefaciens/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Nariz/microbiologia
Doenças Nasais/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMicm1604459


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[PMID]:28040161
[Au] Autor:Wang GY; Wang HH; Han YW; Xing T; Ye KP; Xu XL; Zhou GH
[Ad] Endereço:Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
[Ti] Título:Evaluation of the spoilage potential of bacteria isolated from chilled chicken in vitro and in situ.
[So] Source:Food Microbiol;63:139-146, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microorganisms play an important role in the spoilage of chilled chicken. In this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. Four isolates, namely Aeromonas salmonicida 35, Pseudomonas fluorescens H5, Pseudomonas fragi H8 and Serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage potential in situ. The in vitro studies showed that A. salmonicida 35 displayed the strongest proteolytic activity against both sarcoplasmic and myofibrillar proteins. However, the major spoilage isolate in situ was P. fragi H8, which exhibited a fast growth rate, slime formation and increased pH and total volatile basic nitrogen (TVBN) on chicken breast fillets. The relative amounts of volatile organic compounds (VOCs) originating from the microorganisms, including alcohols, aldehydes, ketones and several sulfur compounds, increased during storage. In sum, this study demonstrated the characteristics of 4 potential spoilage bacteria on chilled yellow-feather chicken and provides a simple and convenient method to assess spoilage bacteria during quality management.
[Mh] Termos MeSH primário: Aeromonas salmonicida/metabolismo
Galinhas/microbiologia
Aves Domésticas/microbiologia
Pseudomonas/metabolismo
Refrigeração
Serratia liquefaciens/metabolismo
[Mh] Termos MeSH secundário: Aeromonas salmonicida/crescimento & desenvolvimento
Aeromonas salmonicida/isolamento & purificação
Animais
Microbiologia de Alimentos
Armazenamento de Alimentos
Proteólise
Pseudomonas/crescimento & desenvolvimento
Pseudomonas fluorescens/crescimento & desenvolvimento
Pseudomonas fluorescens/isolamento & purificação
Pseudomonas fluorescens/metabolismo
Pseudomonas fragi/crescimento & desenvolvimento
Pseudomonas fragi/isolamento & purificação
Pseudomonas fragi/metabolismo
Serratia liquefaciens/crescimento & desenvolvimento
Serratia liquefaciens/isolamento & purificação
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:27580323
[Au] Autor:Ikumapayi UN; Kanteh A; Manneh J; Lamin M; Mackenzie GA
[Ad] Endereço:Medical Research Council Unit, Fajara, Gambia. uikumapayi@mrc.gm.
[Ti] Título:An outbreak of Serratia liquefaciens at a rural health center in The Gambia.
[So] Source:J Infect Dev Ctries;10(8):791-8, 2016 Aug 31.
[Is] ISSN:1972-2680
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Healthcare-associated infections (HAIs) are better documented in developed than in developing countries. There are emerging reports regarding the high frequency of HAIs in developing countries. We aimed to report an outbreak of an HAI caused by Serratia liquefaciens at a rural health center in The Gambia. METHODOLOGY: Following an abrupt increase in the isolation of S. liquefaciens in clinical samples, laboratory and clinical consumables, as well as staff, were screened for contamination with S. liquefaciens. Conventional microbiological techniques and biochemical identification tests were used. A phenotypic typing was achieved using the Kirby-Bauer antibiotic susceptibility method. Strategies to control the outbreak were implemented. RESULTS: A total of 794 samples were processed during the outbreak; 44 (6%) grew S. liquefaciens. Five (25%) of the 20 suspected contaminated materials (hospital consumables and equipment) screened yielded growth of the organism. The primary source of the outbreak was hospital consumables. Three (7%) of the 44 infected children died with no other known cause than S. liquefaciens infection. Ninety-nine percent similarity of the antibiogram phenotypic typing suggests the isolates were from the same clonal origin. The outbreak was successfully controlled after the removal and sterilization of the respective contaminated fluids and equipment. CONCLUSIONS: This HAI was caused by poor practice in the preparation of medications for nebulization and intravenous infusion, hygiene practices, and a lack of awareness among staff about infection control. We recommend further studies to delineate the role played by HAIs in the developing world.
[Mh] Termos MeSH primário: Infecção Hospitalar/epidemiologia
Surtos de Doenças
Microbiologia Ambiental
Infecções por Serratia/epidemiologia
Serratia liquefaciens/isolamento & purificação
[Mh] Termos MeSH secundário: Atitude do Pessoal de Saúde
Técnicas Bacteriológicas
Pré-Escolar
Infecção Hospitalar/microbiologia
Contaminação de Medicamentos
Contaminação de Equipamentos
Feminino
Gâmbia/epidemiologia
Seres Humanos
Lactente
Recém-Nascido
Masculino
Competência Profissional
Serviços de Saúde Rural
Infecções por Serratia/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.3855/jidc.7184


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[PMID]:27138047
[Au] Autor:Zelaya-Molina LX; Hernández-Soto LM; Guerra-Camacho JE; Monterrubio-López R; Patiño-Siciliano A; Villa-Tanaca L; Hernández-Rodríguez C
[Ad] Endereço:Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala s/n. Col. Sto. Tomás, 11340, Mexico, D.F., Mexico.
[Ti] Título:Ammonia-Oligotrophic and Diazotrophic Heavy Metal-Resistant Serratia liquefaciens Strains from Pioneer Plants and Mine Tailings.
[So] Source:Microb Ecol;72(2):324-46, 2016 Aug.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mine tailings are man-made environments characterized by low levels of organic carbon and assimilable nitrogen, as well as moderate concentrations of heavy metals. For the introduction of nitrogen into these environments, a key role is played by ammonia-oligotrophic/diazotrophic heavy metal-resistant guilds. In mine tailings from Zacatecas, Mexico, Serratia liquefaciens was the dominant heterotrophic culturable species isolated in N-free media from bulk mine tailings as well as the rhizosphere, roots, and aerial parts of pioneer plants. S. liquefaciens strains proved to be a meta-population with high intraspecific genetic diversity and a potential to respond to these extreme conditions. The phenotypic and genotypic features of these strains reveal the potential adaptation of S. liquefaciens to oligotrophic and nitrogen-limited mine tailings with high concentrations of heavy metals. These features include ammonia-oligotrophic growth, nitrogen fixation, siderophore and indoleacetic acid production, phosphate solubilization, biofilm formation, moderate tolerance to heavy metals under conditions of diverse nitrogen availability, and the presence of zntA, amtB, and nifH genes. The acetylene reduction assay suggests low nitrogen-fixing activity. The nifH gene was harbored in a plasmid of ∼60 kb and probably was acquired by a horizontal gene transfer event from Klebsiella variicola.
[Mh] Termos MeSH primário: Amônia/análise
Metais Pesados/análise
Mineração
Filogenia
Raízes de Plantas/microbiologia
Serratia liquefaciens/classificação
[Mh] Termos MeSH secundário: Biofilmes
DNA Bacteriano/genética
Genes Bacterianos
Variação Genética
Concentração de Íons de Hidrogênio
Ácidos Indolacéticos/análise
Metagenômica
México
Testes de Sensibilidade Microbiana
Fixação de Nitrogênio
RNA Ribossômico 16S/genética
Rizosfera
Serratia liquefaciens/genética
Serratia liquefaciens/isolamento & purificação
Microbiologia do Solo
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Indoleacetic Acids); 0 (Metals, Heavy); 0 (RNA, Ribosomal, 16S); 6U1S09C61L (indoleacetic acid); 7664-41-7 (Ammonia)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-016-0771-3


  9 / 52 MEDLINE  
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[PMID]:27125750
[Au] Autor:Tian JH; Pourcher AM; Peu P
[Ad] Endereço:Irstea, UR OPAALE, 17 Avenue de Cucillé-CS 64427, F-35044, Rennes, France.
[Ti] Título:Isolation of bacterial strains able to metabolize lignin and lignin-related compounds.
[So] Source:Lett Appl Microbiol;63(1):30-7, 2016 Jul.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: In this study, we identified five strains isolated from soil and sediments able to degrade kraft lignin, aromatic dyes and lignin derivatives. Using 16S rRNA gene sequencing, the isolates were identified as Serratia sp. JHT01, Serratia liquefacien PT01, Pseudomonas chlororaphis PT02, Stenotrophomonas maltophilia PT03 and Mesorhizobium sp. PT04. All the isolates showed significant growth on lignin with no water-extractable compounds. Synthetic aromatic dyes were used to assess the presence of oxidative enzymes. All the isolates were able to use the thiazine dye Methylene blue and the anthraquinone dye Remazol Brilliant Blue R as the sole carbon source. Guaiacol, veratryl alcohol and biphenyl were also mineralized by all the strains isolated. These results suggest they could be used for the treatment of aromatic pollutants and for the degradation of the lignocellulosic biomass. SIGNIFICANCE AND IMPACT OF THE STUDY: The valorization of waste lignin and lignocellulosic biomass by biocatalysis opens up new possibilities for the production of value-added substituted aromatics, biofuel and for the treatment of aromatic pollutants. Bacteria with ligninolytic potential could be a source of novel enzymes for controlled lignin depolymerization. In this work, five soil bacteria were isolated and studied. Every isolate showed significant growth on lignin and was able to degrade several lignin monomers and ligninolytic indicator dyes. They could thus be a source of novel ligninolytic enzymes as well as candidates for a bacterial consortium for the delignification of lignocellulosic biomass.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Corantes/metabolismo
Lignina/metabolismo
Mesorhizobium/metabolismo
Pseudomonas chlororaphis/metabolismo
Serratia liquefaciens/metabolismo
Stenotrophomonas maltophilia/metabolismo
[Mh] Termos MeSH secundário: Antraquinonas/metabolismo
Álcoois Benzílicos/metabolismo
Biocombustíveis
Biomassa
Compostos de Bifenilo/metabolismo
Guaiacol/metabolismo
Mesorhizobium/genética
Mesorhizobium/isolamento & purificação
Azul de Metileno/metabolismo
Pseudomonas chlororaphis/genética
Pseudomonas chlororaphis/isolamento & purificação
RNA Ribossômico 16S/genética
Serratia liquefaciens/genética
Serratia liquefaciens/isolamento & purificação
Microbiologia do Solo
Stenotrophomonas maltophilia/genética
Stenotrophomonas maltophilia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Benzyl Alcohols); 0 (Biofuels); 0 (Biphenyl Compounds); 0 (Coloring Agents); 0 (RNA, Ribosomal, 16S); 11132-73-3 (lignocellulose); 2L9GJK6MGN (diphenyl); 6JKA7MAH9C (Guaiacol); 8068-05-1 (Kraft lignin); 9005-53-2 (Lignin); L51IMM9UP9 (Remazol Brilliant Blue R); MB4T4A711H (veratryl alcohol); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160430
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12581


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[PMID]:27059494
[Au] Autor:Iiyama K; Lee JM; Tatsuke T; Mon H; Kusakabe T
[Ad] Endereço:Laboratory of Insect Pathology and Microbial Control, Faculty of Agriculture, Graduate School, Institute of Biological Control, Kyushu University, Fukuoka, Japan. iiyama@grt.kyushu-u.ac.jp.
[Ti] Título:Expression and Characterization of Recombinant Serratia liquefaciens Nucleases Produced with Baculovirus-mediated Silkworm Expression System.
[So] Source:Mol Biotechnol;58(6):393-403, 2016 Jun.
[Is] ISSN:1559-0305
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Baculovirus-Bombyx mori protein expression system has mainly been used for translation of eukaryotic proteins. In contrast, information pertaining to bacterial protein expression using this system is not sufficient. Therefore, recombinant nucleases from Serratia liquefaciens (rSlNucAs) were expressed in a Baculovirus-B. mori protein expression system. rSlNucAs containing the native signal peptide (rSlNucA-NSP) or silkworm 30-K signal peptide (rSlNucA-30K) at the NH2-terminus were constructed to enable secretion into the extracellular fraction. Both rSlNucA-30K and rSlNucA-NSP were successfully secreted into hemolymph of B. mori larvae. Affinity-purified rSlNucAs showed high nuclease activity. Optimum pH was 7.5 and half of maximum activity was maintained between pH 7.0 and 9.5. Optimum temperature was 35 °C. rSlNucAs showed sufficient activity in twofold-diluted radioimmunoprecipitation assay buffer and undiluted, mild lysis buffer. Genomic DNA of Escherichia coli was efficiently digested by rSlNucAs in the bacterial lysate. The results in this study suggest that rSlNucAs expressed by the Baculovirus-B. mori protein expression system will be a useful tool in molecular biology. Functional recombinant protein of bacteria was produced by Baculovirus-B. mori protein expression system. This system may be highly suitable for bacterial extracellular protein secreted via Sec pathway.
[Mh] Termos MeSH primário: Baculoviridae/genética
Bombyx/virologia
Desoxirribonucleases/genética
Desoxirribonucleases/metabolismo
Serratia liquefaciens/enzimologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Baculoviridae/enzimologia
Clonagem Molecular
Desoxirribonucleases/química
Expressão Gênica
Concentração de Íons de Hidrogênio
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Serratia liquefaciens/genética
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160410
[St] Status:MEDLINE
[do] DOI:10.1007/s12033-016-9937-y



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