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[PMID]:29303698
[Au] Autor:Adhikary S; Bisgaard M; Nicklas W; Christensen H
[Ad] Endereço:1​Department of Veterinary Animal Sciences, University of Copenhagen, 4 Stigbøjlen, DK-1870 Frederiksberg C, Denmark.
[Ti] Título:Reclassification of Bisgaard taxon 5 as Caviibacterium pharyngocola gen. nov., sp. nov. and Bisgaard taxon 7 as Conservatibacter flavescens gen. nov., sp. nov.
[So] Source:Int J Syst Evol Microbiol;68(2):643-650, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A total of 29 strains mainly from guinea pigs were investigated by a polyphasic approach that included previously published data. The strains were classified as Bisgaard taxa 5 and 7 by comparison of phenotypic characteristics and the strains showed typical cultural characteristics for members of family Pasteurellaceae and the strains formed two monophyletic groups based on 16S rRNA gene sequence comparison. Partial rpoB sequence analysis as well as published data on DNA-DNA hybridization showed high genotypic relationships within both groups. A new genus with one species, Caviibacterium pharyngocola gen. nov., sp. nov., is proposed to accommodate members of taxon 5 of Bisgaard, whereas members of taxon 7 are proposed as Conservatibacter flavescens gen. nov., sp. nov. The two genera are clearly separated by phenotype from each other and from existing genera of the family Pasteurellaceae. The type strain of Caviibacterium pharyngocola is 7.3 (=CCUG 16493 =DSM 105478 ) and the type strain of Conservatibacter flavescens is 7.4 (=CCUG 24852 =DSM 105479 =HIM 794-7 ), both were isolated from the pharynx of guinea pigs.
[Mh] Termos MeSH primário: Cobaias/microbiologia
Pasteurellaceae/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Genes Bacterianos
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002558


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[PMID]:28827826
[Au] Autor:Dassanayake RP; Falkenberg SM; Briggs RE; Tatum FM; Sacco RE
[Ad] Endereço:Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America.
[Ti] Título:Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni.
[So] Source:PLoS One;12(8):e0183610, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine NK-lysins, which are functionally and structurally similar to human granulysin and porcine NK-lysin, are predominantly found in the granules of cytotoxic T-lymphocytes and NK-cells. Although antimicrobial activity of bovine NK-lysin has been assessed for several bacterial pathogens, not all the important bacterial pathogens that are involved in the bovine respiratory disease complex have been studied. Therefore the objective of the present study was to evaluate the antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni. Four, 30-mer peptides corresponding to the functional region of NK-lysin helices 2 and 3 were synthesized and assessed for antibacterial activity on four bovine pneumonic H. somni isolates. Although there were some differences in the efficiency of bactericidal activity among the NK-lysin peptides at lower concentrations (2-5 µM), all four peptides effectively killed most H. somni isolates at higher concentrations (10-30 µM) as determined by a bacterial killing assay. Confocal microscopic and flow cytometric analysis of Live/Dead Baclight stained H. somni (which were preincubated with NK-lysin peptides) were consistent with the killing assay findings and suggest NK-lysin peptides are bactericidal for H. somni. Among the four peptides, NK2A-derived peptide consistently showed the highest antimicrobial activity against all four H. somni isolates. Electron microscopic examination of H. somni following incubation with NK-lysin revealed extensive cell membrane damage, protrusions of outer membranes, and cytoplasmic content leakage. Taken together, the findings from this study clearly demonstrate the antimicrobial activity of all four bovine NK-lysin-derived peptides against bovine H. somni isolates.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Complexo Respiratório Bovino/prevenção & controle
Pasteurellaceae/efeitos dos fármacos
Peptídeos/farmacologia
Proteolipídeos/farmacologia
Sistema Respiratório/microbiologia
[Mh] Termos MeSH secundário: Animais
Complexo Respiratório Bovino/microbiologia
Bovinos
Microscopia Eletrônica de Transmissão
Proteolipídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (NK-lysin); 0 (Peptides); 0 (Proteolipids)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183610


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[PMID]:28708832
[Au] Autor:Butler CJ; Edwards WH; Jennings-Gaines JE; Killion HJ; Wood ME; McWhirter DE; Paterson JT; Proffitt KM; Almberg ES; White PJ; Rotella JJ; Garrott RA
[Ad] Endereço:Fish and Wildlife Ecology and Management Program, Department of Ecology, Montana State University, Bozeman, Montana, United States of America.
[Ti] Título:Assessing respiratory pathogen communities in bighorn sheep populations: Sampling realities, challenges, and improvements.
[So] Source:PLoS One;12(7):e0180689, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory disease has been a persistent problem for the recovery of bighorn sheep (Ovis canadensis), but has uncertain etiology. The disease has been attributed to several bacterial pathogens including Mycoplasma ovipneumoniae and Pasteurellaceae pathogens belonging to the Mannheimia, Bibersteinia, and Pasteurella genera. We estimated detection probability for these pathogens using protocols with diagnostic tests offered by a fee-for-service laboratory and not offered by a fee-for-service laboratory. We conducted 2861 diagnostic tests on swab samples collected from 476 bighorn sheep captured across Montana and Wyoming to gain inferences regarding detection probability, pathogen prevalence, and the power of different sampling methodologies to detect pathogens in bighorn sheep populations. Estimated detection probability using fee-for-service protocols was less than 0.50 for all Pasteurellaceae and 0.73 for Mycoplasma ovipneumoniae. Non-fee-for-service Pasteurellaceae protocols had higher detection probabilities, but no single protocol increased detection probability of all Pasteurellaceae pathogens to greater than 0.50. At least one protocol resulted in an estimated detection probability of 0.80 for each pathogen except Mannheimia haemolytica, for which the highest detection probability was 0.45. In general, the power to detect Pasteurellaceae pathogens at low prevalence in populations was low unless many animals were sampled or replicate samples were collected per animal. Imperfect detection also resulted in low precision when estimating prevalence for any pathogen. Low and variable detection probabilities for respiratory pathogens using live-sampling protocols may lead to inaccurate conclusions regarding pathogen community dynamics and causes of bighorn sheep respiratory disease epizootics. We recommend that agencies collect multiples samples per animal for Pasteurellaceae detection, and one sample for Mycoplasma ovipneumoniae detection from at least 30 individuals to reliably detect both Pasteurellaceae and Mycoplasma ovipneumoniae at the population-level. Availability of PCR diagnostic tests to wildlife management agencies would improve the ability to reliably detect Pasteurellaceae in bighorn sheep populations.
[Mh] Termos MeSH primário: Infecções Respiratórias/diagnóstico
Doenças dos Ovinos/diagnóstico
[Mh] Termos MeSH secundário: Animais
DNA Bacteriano/metabolismo
Mycoplasma ovipneumoniae/genética
Mycoplasma ovipneumoniae/isolamento & purificação
Pasteurellaceae/genética
Pasteurellaceae/isolamento & purificação
Densidade Demográfica
Prevalência
Reação em Cadeia da Polimerase em Tempo Real
Infecções Respiratórias/epidemiologia
Infecções Respiratórias/microbiologia
Ovinos
Doenças dos Ovinos/epidemiologia
Doenças dos Ovinos/microbiologia
Carneiro da Montanha
Manejo de Espécimes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180689


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[PMID]:28629498
[Au] Autor:Adhikary S; Nicklas W; Bisgaard M; Boot R; Kuhnert P; Waberschek T; Aalbæk B; Korczak B; Christensen H
[Ad] Endereço:1​Department of Veterinary Disease Biology, University of Copenhagen, 4 Stigbøjlen, DK-1870 Frederiksberg C, Denmark.
[Ti] Título:Rodentibacter gen. nov. including Rodentibacter pneumotropicus comb. nov., Rodentibacter heylii sp. nov., Rodentibacter myodis sp. nov., Rodentibacter ratti sp. nov., Rodentibacter heidelbergensis sp. nov., Rodentibacter trehalosifermentans sp. nov., Rodentibacter rarus sp. nov., Rodentibacter mrazii and two genomospecies.
[So] Source:Int J Syst Evol Microbiol;67(6):1793-1806, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Rodentibacter gen. nov. is proposed based on isolation and phenotypic characterization of strains, predominantly from rodents. The strains showed 86 % or higher rpoB gene sequence similarity and indicated a genus-level relationship within Pasteurellaceae. The strains compared at 16S rRNA gene sequence level showed 93.8 % or higher similarity, and their genus-level relationship within Pasteurellaceae was confirmed by phenotypic analysis. The type species Rodentibacter pneumotropicus comb. nov. is reclassified from [Pasteurella] pneumotropica with type strain NCTC 8141T (=CCUG 12398T). Whole genomic comparison allowed the estimation of DNA-DNA renaturation. Rodentibacter heylii sp. nov. was proposed for a group that included the biovar Heyl of [Pasteurella] pneumotropica with the type strain ATCC 12555T (=CCUG 998T). A group was proposed as Rodentibacter ratti sp. nov., which included the taxon 22 of Bisgaard; the type strain is F75T (=CCUG 69665T=DSM 103977T). Taxon 41 of Bisgaard was proposed as Rodentibacter myodis sp. nov. with type strain Ac151T (=CCUG 69666T=DSM 103994T). Rodentibacter heidelbergensis sp. nov. included the type strain 1996025094T (=Ac69T) (=CCUG 69667T=DSM 103978T). A group strains of was proposed as Rodentibacter trehalosifermentans sp. nov. with type strain H1987082031T (=CCUG 69668T=DSM 104075T). Two strains including the reference strain of taxon 17 of Bisgaard that showed 16S rRNA gene similarity of 97.3 % were proposed as Rodentibacter rarus sp. nov. 2325/79T (=CCUG 17206T=DSM 103980T). Rodentibacter mrazii sp. nov. was proposed with type strain Ppn418T (Bisgaard taxon 21) (=CCUG 69669T=DSM 103979T). The eight species could be separated based on phenotypic characteristics such as NAD requirement, ornithine decarboxylase and indole formation, α-glucosidase, ß-galactosidase and in acid formation from (+)-l-arabinose, (-)-d-ribose, (+)-d-xylose, myo-inositol, (-)-d-mannitol, lactose, melibiose and trehalose. Forty-six strains including taxon 48 of Bisgaard formed a monophyletic group by rpoB and 16S rRNA gene sequence analysis, but could not be separated phenotypically from R. pneumotropicus and R. heylii, and it was left as an unnamed genomospecies 1 of Rodentibacter with reference strain Ppn416. Another taxon that included 13 strains, mainly isolated from Apodemus sylvaticus, could not be separated phenotypically from R. pneumotropicus or R. heylii and was designated as genomospecies 2. Strain Ppn85 with 95 % or less rpoB gene sequence similarity and with 16S rRNA gene sequence similarity of 97 % or less to the other members of Rodentibacter was left as an unnamed singleton.
[Mh] Termos MeSH primário: Pasteurellaceae/classificação
Filogenia
Roedores/microbiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Genes Bacterianos
Hibridização de Ácido Nucleico
Pasteurellaceae/genética
Pasteurellaceae/isolamento & purificação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001866


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[PMID]:28425146
[Au] Autor:Van Driessche L; Valgaeren BR; Gille L; Boyen F; Ducatelle R; Haesebrouck F; Deprez P; Pardon B
[Ad] Endereço:Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
[Ti] Título:A Deep Nasopharyngeal Swab Versus Nonendoscopic Bronchoalveolar Lavage for Isolation of Bacterial Pathogens from Preweaned Calves With Respiratory Disease.
[So] Source:J Vet Intern Med;31(3):946-953, 2017 May.
[Is] ISSN:1939-1676
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nonendoscopic bronchoalveolar lavage (BAL) is a practical alternative for a deep nasopharyngeal swab (DNS) to sample the airways of a large number of calves in a short period of time. The extent of commensal overgrowth and agreement of BAL with DNS culture results in preweaned calves are unknown. OBJECTIVES: To compare commensal overgrowth and bacterial culture results between DNS and BAL samples. ANIMALS: A total of 183 preweaned calves (144 with bovine respiratory disease and 39 healthy animals). METHODS: Cross-sectional study. Deep nasopharyngeal swab and BAL samples were taken from each calf and cultured to detect Pasteurellaceae and Mycoplasma bovis. Agreement and associations between culture results of DNS and BAL samples were determined by kappa statistics and logistic regression. RESULTS: Bronchoalveolar lavage samples were less often polymicrobial, more frequently negative and yielded more pure cultures compared to DNS, leading to a clinically interpretable culture result in 79.2% of the cases compared to only in 31.2% of the DNS samples. Isolation rates were lower in healthy animals, but not different between DNS and BAL samples. Only Histophilus somni was more likely to be isolated from BAL samples. In clinical cases, a polymicrobial DNS culture result did not increase the probability of a polymicrobial BAL result by ≥30%, nor did it influence the probability of a negative culture. A significant herd effect was noted for all observed relationships. CONCLUSIONS AND CLINICAL RELEVANCE: Nonendoscopic BAL samples are far less overgrown by bacteria compared to DNS samples under the conditions of this study, facilitating clinical interpretation and resulting in a higher return on investment in bacteriologic culturing.
[Mh] Termos MeSH primário: Complexo Respiratório Bovino/microbiologia
Líquido da Lavagem Broncoalveolar/microbiologia
Mycoplasma bovis/isolamento & purificação
Nasofaringe/microbiologia
Pasteurellaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Complexo Respiratório Bovino/diagnóstico
Lavagem Broncoalveolar/veterinária
Bovinos
Estudos Transversais
Infecções por Mycoplasma/diagnóstico
Infecções por Mycoplasma/veterinária
Infecções por Pasteurellaceae/diagnóstico
Infecções por Pasteurellaceae/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1111/jvim.14668


  6 / 398 MEDLINE  
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[PMID]:28301245
[Au] Autor:Bisgaard M; Xin D; Bertelsen MF; Bojesen AM; Christensen H
[Ad] Endereço:A Bisgaard Consulting, Viby, Denmark.
[Ti] Título:Prevalence of Taxa of Pasteurellaceae Among Populations of Healthy Captive Psittacine Birds.
[So] Source:Avian Dis;61(1):102-106, 2017 Mar.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sixty-two strains of Pasteurellaceae-like bacteria were isolated from the tracheas of 87 clinically healthy psittacine birds in two Danish zoos. The isolates were identified by a combination of rpoB and 16S rRNA gene sequencing and by matrix-assisted laser desorption-ionization time of flight. Twenty-eight strains belonged to the genus Volucribacter or were related to this genus and to the unnamed taxon 34 of Bisgaard, and 28 strains were related to the unnamed taxon 44 of Bisgaard. Four strains were identified as Pasteurella multocida , two isolates were classified with the related taxon 45 of Bisgaard, and a single isolate was classified as Pasteurella sp. The investigation documented an unrecognized reservoir of rarely reported and unclassified or unnamed species of Pasteurellaceae-like bacteria in psittacine birds. The results were in accordance with a recent report on isolation of Pasteurellaceae from diseased psittacine birds, and the investigation documented that the same taxa of Pasteurellaceae-like bacteria can be isolated from apparently healthy birds as well as from diseased birds.
[Mh] Termos MeSH primário: Doenças das Aves/virologia
Infecções por Pasteurellaceae/veterinária
Pasteurellaceae/classificação
Pasteurellaceae/isolamento & purificação
Psittaciformes/virologia
[Mh] Termos MeSH secundário: Animais
DNA Bacteriano/genética
Pasteurellaceae/genética
Infecções por Pasteurellaceae/virologia
Filogenia
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1637/11522-103016-Reg


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[PMID]:28301236
[Au] Autor:Paudel S; Hess M; Hess C
[Ad] Endereço:Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, 1210, Vienna, Austria.
[Ti] Título:Coinfection of Avibacterium paragallinarum and Gallibacterium anatis in Specific-Pathogen-Free Chickens Complicates Clinical Signs of Infectious Coryza, Which Can Be Prevented by Vaccination.
[So] Source:Avian Dis;61(1):55-63, 2017 Mar.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Avibacterium paragallinarum and Gallibacterium anatis are recognized bacterial pathogens both infecting the respiratory tract of chickens. The present study investigated outcomes of their coinfection by elucidating clinical signs, pathologic lesions, and bacteriologic findings. Additionally, the efficacy of a commercially available vaccine to prevent diseases caused by A. paragallinarum and G. anatis was evaluated. Birds inoculated with G. anatis alone did not present any clinical signs and gross pathologic lesions in the respiratory tract. However, clinical signs of infectious coryza were reproduced in nonvaccinated birds that were challenged with A. paragallinarum alone or together with G. anatis . Such clinical signs were more severe in the coinfected group, including the death of four birds. Some of the birds that were vaccinated and challenged showed mild clinical signs at 7 days postinfection (dpi). Inflammation of sinus infraorbitalis was the most prominent gross pathologic lesion found in the respiratory tract of nonvaccinated birds inoculated either with A. paragallinarum and G. anatis or A. paragallinarum alone. In the reproductive tract, hemorrhagic follicles were observed in nonvaccinated birds that were infected either with G. anatis alone or together with A. paragallinarum . In vaccinated birds, no gross pathologic lesions were found except in one bird that was coinfected with both the pathogens characterized by mucoid tracheitis. Bacteriologic investigations revealed that multiplication of G. anatis at 7 dpi was supported by the coinfection with A. paragallinarum . Altogether, it can be concluded that simultaneous infection of A. paragallinarum and G. anatis can increase the severities of disease conditions in chickens. In such a scenario, vaccination appears to be an effective tool for prevention of the disease, as protection was conferred based on clinical, pathologic, bacteriologic, and serologic data.
[Mh] Termos MeSH primário: Vacinas Bacterianas/administração & dosagem
Coinfecção/microbiologia
Infecções por Haemophilus/prevenção & controle
Haemophilus paragallinarum/imunologia
Infecções por Pasteurellaceae/prevenção & controle
Pasteurellaceae/imunologia
Doenças das Aves Domésticas/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Vacinas Bacterianas/imunologia
Galinhas
Coinfecção/patologia
Coinfecção/prevenção & controle
Infecções por Haemophilus/microbiologia
Infecções por Haemophilus/patologia
Haemophilus paragallinarum/genética
Haemophilus paragallinarum/fisiologia
Pasteurellaceae/genética
Pasteurellaceae/fisiologia
Infecções por Pasteurellaceae/microbiologia
Infecções por Pasteurellaceae/patologia
Doenças das Aves Domésticas/microbiologia
Doenças das Aves Domésticas/patologia
Organismos Livres de Patógenos Específicos
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Vaccines)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1637/11481-081016-Reg


  8 / 398 MEDLINE  
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[PMID]:28282407
[Au] Autor:Drew ML; Weiser GC
[Ad] Endereço:Wildlife Health Laboratory, Idaho Department of Fish and Game, Eagle, Idaho, United States of America.
[Ti] Título:Potential disease agents in domestic goats and relevance to bighorn sheep (Ovis canadensis) management.
[So] Source:PLoS One;12(3):e0173396, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Domestic goats are raised for meat, milk and hair production, in herds for rangeland weed control, and as pack animals. Domestic sheep, goats and wild bighorn sheep are all susceptible to a multifactorial pneumonia. We sampled 43 herd goats from 7 herds and 48 pack goats from 11 herds for viral and bacterial serology, parasitology, and Pasteurellaceae microbiology. The goats in this study were in generally good health, although most goats did harbor various pathogens and parasites including several bacteria, specifically Pasteurellaceae, which have been associated with pneumonia in free-ranging bighorn sheep. It is not known if domestic goats can transmit the Pasteurellaceae or other pathogens found in this study readily to wild bighorn sheep. However, due the possibility of transmission, domestic goats in areas in or near bighorn sheep habitat should be managed to minimize the risk of spreading disease agents to bighorn sheep.
[Mh] Termos MeSH primário: Cabras/microbiologia
Ovinos/microbiologia
[Mh] Termos MeSH secundário: Animais
Animais Domésticos
Anticorpos Antivirais/sangue
Ecossistema
Fezes/parasitologia
Doenças das Cabras/microbiologia
Doenças das Cabras/parasitologia
Doenças das Cabras/virologia
Cabras/parasitologia
Cabras/virologia
Pasteurellaceae/isolamento & purificação
Ovinos/parasitologia
Ovinos/virologia
Doenças dos Ovinos/microbiologia
Doenças dos Ovinos/parasitologia
Doenças dos Ovinos/virologia
Carneiro da Montanha/microbiologia
Carneiro da Montanha/parasitologia
Carneiro da Montanha/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173396


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[PMID]:28240958
[Au] Autor:Capik SF; White BJ; Lubbers BV; Apley MD; DeDonder KD; Larson RL; Harhay GP; Chitko-McKown CG; Harhay DM; Kalbfleisch TS; Schuller G; Clawson ML
[Ti] Título:Comparison of the diagnostic performance of bacterial culture of nasopharyngeal swab and bronchoalveolar lavage fluid samples obtained from calves with bovine respiratory disease.
[So] Source:Am J Vet Res;78(3):350-358, 2017 Mar.
[Is] ISSN:1943-5681
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To compare predictive values, extent of agreement, and gamithromycin susceptibility between bacterial culture results of nasopharyngeal swab (NPS) and bronchoalveolar lavage fluid (BALF) samples obtained from calves with bovine respiratory disease (BRD). ANIMALS 28 beef calves with clinical BRD. PROCEDURES Pooled bilateral NPS samples and BALF samples were obtained for bacterial culture from calves immediately before and at various times during the 5 days after gamithromycin (6 mg/kg, SC, once) administration. For each culture-positive sample, up to 12 Mannheimia haemolytica, 6 Pasteurella multocida, and 6 Histophilus somni colonies underwent gamithromycin susceptibility testing. Whole-genome sequencing was performed on all M haemolytica isolates. For paired NPS and BALF samples collected 5 days after gamithromycin administration, the positive and negative predictive values for culture results of NPS samples relative to those of BALF samples and the extent of agreement between the sampling methods were determined. RESULTS Positive and negative predictive values of NPS samples were 67% and 100% for M haemolytica, 75% and 100% for P multocida, and 100% and 96% for H somni. Extent of agreement between results for NPS and BALF samples was substantial for M haemolytica (κ, 0.71) and H somni (κ, 0.78) and almost perfect for P multocida (κ, 0.81). Gamithromycin susceptibility varied within the same sample and between paired NPS and BALF samples. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated culture results of NPS and BALF samples from calves with BRD should be interpreted cautiously considering disease prevalence within the population, sample collection relative to antimicrobial administration, and limitations of diagnostic testing methods.
[Mh] Termos MeSH primário: Líquido da Lavagem Broncoalveolar/microbiologia
Doenças dos Bovinos/diagnóstico
Nasofaringe/microbiologia
Doenças Respiratórias/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/microbiologia
Genoma Bacteriano/genética
Macrolídeos/farmacologia
Masculino
Mannheimia haemolytica/efeitos dos fármacos
Mannheimia haemolytica/genética
Mannheimia haemolytica/isolamento & purificação
Pasteurella multocida/efeitos dos fármacos
Pasteurella multocida/genética
Pasteurella multocida/isolamento & purificação
Pasteurellaceae/efeitos dos fármacos
Pasteurellaceae/genética
Pasteurellaceae/isolamento & purificação
Valor Preditivo dos Testes
Doenças Respiratórias/diagnóstico
Doenças Respiratórias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Macrolides); ZE856183S0 (gamithromycin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.2460/ajvr.78.3.350


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[PMID]:27724044
[Au] Autor:Hess M
[Ad] Endereço:a Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health , University of Veterinary Medicine , Vienna , Austria.
[Ti] Título:Commensal or pathogen - a challenge to fulfil Koch's Postulates.
[So] Source:Br Poult Sci;58(1):1-12, 2017 Feb.
[Is] ISSN:1466-1799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:1. Infectious diseases have a large impact on poultry health and economics. Elucidating the pathogenesis of a certain disease is crucial to implement control strategies. 2. Multiplication of a pathogen and its characterisation in vitro are basic requirements to perform experimental studies. However, passaging of the pathogen in vitro can influence the pathogenicity, a process targeted for live vaccine development, but limits the reproduction of clinical signs. 3. Numerous factors can influence the outcome of experimental infections with some importance on the pathogen, application route and host as exemplarily outlined for Histomonas meleagridis, Gallibacterium anatis and fowl aviadenoviruses (FAdVs). 4. In future, more comprehensive and detailed settings are needed to obtain as much information as possible from animal experiments. Processing of samples with modern diagnostic tools provides the option to closely monitor the host-pathogen interaction.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno
Doenças das Aves Domésticas/microbiologia
Simbiose
[Mh] Termos MeSH secundário: Infecções por Adenoviridae/história
Infecções por Adenoviridae/veterinária
Animais
Aviadenovirus/classificação
Galinhas
Inglaterra
Adenovirus A das Aves
História do Século XIX
História do Século XX
História do Século XXI
Pasteurellaceae/classificação
Infecções por Pasteurellaceae/história
Infecções por Pasteurellaceae/microbiologia
Infecções por Pasteurellaceae/veterinária
Doenças das Aves Domésticas/história
Doenças das Aves Domésticas/parasitologia
Infecções Protozoárias em Animais/história
Infecções Protozoárias em Animais/parasitologia
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; LECTURES
[Ps] Nome de pessoa como assunto:Koch R
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE
[do] DOI:10.1080/00071668.2016.1245849



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