Base de dados : MEDLINE
Pesquisa : B03.440.450.600.450 [Categoria DeCS]
Referências encontradas : 2226 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 223 ir para página                         

  1 / 2226 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28319121
[Au] Autor:Liu A; Archer AM; Biggs MB; Papin JA
[Ad] Endereço:Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, United States of America.
[Ti] Título:Growth-altering microbial interactions are responsive to chemical context.
[So] Source:PLoS One;12(3):e0164919, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microbial interactions are ubiquitous in nature, and are equally as relevant to human wellbeing as the identities of the interacting microbes. However, microbial interactions are difficult to measure and characterize. Furthermore, there is growing evidence that they are not fixed, but dependent on environmental context. We present a novel workflow for inferring microbial interactions that integrates semi-automated image analysis with a colony stamping mechanism, with the overall effect of improving throughput and reproducibility of colony interaction assays. We apply our approach to infer interactions among bacterial species associated with the normal lung microbiome, and how those interactions are altered by the presence of benzo[a]pyrene, a carcinogenic compound found in cigarettes. We found that the presence of this single compound changed the interaction network, demonstrating that microbial interactions are indeed dynamic and responsive to local chemical context.
[Mh] Termos MeSH primário: Interações Microbianas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Processamento Automatizado de Dados
Benzo(a)pireno/toxicidade
Benzopirenos/toxicidade
Carcinógenos
Técnicas de Cultura de Células
Haemophilus/citologia
Haemophilus/efeitos dos fármacos
Haemophilus/fisiologia
Seres Humanos
Processamento de Imagem Assistida por Computador
Pulmão/efeitos dos fármacos
Pulmão/microbiologia
Interações Microbianas/fisiologia
Microbiota/efeitos dos fármacos
Microbiota/fisiologia
Microscopia
Pseudomonas aeruginosa/citologia
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/fisiologia
Staphylococcus aureus/citologia
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (Carcinogens); 3417WMA06D (Benzo(a)pyrene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0164919


  2 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28242259
[Au] Autor:Latham RD; Gell DA; Fairbairn RL; Lyons AB; Shukla SD; Cho KY; Jones DA; Harkness NM; Tristram SG
[Ad] Endereço:University of Tasmania, School of Medicine, Medical Sciences Precinct, Hobart, Tasmania, Australia. Electronic address: roger.latham@utas.edu.au.
[Ti] Título:An isolate of Haemophilus haemolyticus produces a bacteriocin-like substance that inhibits the growth of nontypeable Haemophilus influenzae.
[So] Source:Int J Antimicrob Agents;49(4):503-506, 2017 Apr.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nontypeable Haemophilus influenzae (NTHi) frequently colonises the upper respiratory tract and is an important cause of respiratory infections. Resistance to antibiotics is an emerging trend in NTHi and alternative prevention or treatment strategies are required. Haemophilus haemolyticus is a common commensal occupying the same niche as NTHi and, if able to produce substances that inhibit NTHi growth, may have a role as a probiotic. In this study, ammonium sulphate extracts from broth culture of 100 H. haemolyticus isolates were tested for the presence of substances inhibitory to NTHi using a well diffusion assay. One isolate produced a substance that consistently inhibited the growth of NTHi. The substance was inactivated by protease enzymes and had a molecular size of ca. 30 kDa as determined by size exclusion chromatography. When the substance was tested against bacteria from eight Gram-negative and three Gram-positive genera, only Haemophilus spp. were inhibited. Quantitative PCR testing showed the substance to be different to 'haemocin', the previously described bacteriocin of H. influenzae type b. These molecular characteristics, together with narrow-spectrum activity, suggest the substance may be a novel bacteriocin, and there is potential for this H. haemolyticus isolate to function as a probiotic for reduction of colonisation and subsequent infection with NTHi.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Antibiose
Bacteriocinas/metabolismo
Haemophilus/fisiologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/isolamento & purificação
Proteínas de Bactérias/química
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Bacteriocinas/química
Bacteriocinas/isolamento & purificação
Haemophilus/crescimento & desenvolvimento
Haemophilus/metabolismo
Peso Molecular
Proteólise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Bacteriocins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE


  3 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27367145
[Au] Autor:Nordvang RT; Nyffenegger C; Holck J; Jers C; Zeuner B; Sundekilde UK; Meyer AS; Mikkelsen JD
[Ad] Endereço:Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
[Ti] Título:It All Starts with a Sandwich: Identification of Sialidases with Trans-Glycosylation Activity.
[So] Source:PLoS One;11(7):e0158434, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sialidases (3.2.1.18) may exhibit trans-sialidase activity to catalyze sialylation of lactose if the active site topology is congruent with that of the Trypanosoma cruzi trans-sialidase (EC 2.4.1.-). The present work was undertaken to test the hypothesis that a particular aromatic sandwich structure of two amino acids proximal to the active site of the T. cruzi trans-sialidase infers trans-sialidase activity. On this basis, four enzymes with putative trans-sialidase activity were identified through an iterative alignment from 2909 native sialidases available in GenBank, which were cloned and expressed in Escherichia coli. Of these, one enzyme, SialH, derived from Haemophilus parasuis had an aromatic sandwich structure on the protein surface facing the end of the catalytic site (Phe168; Trp366), and was indeed found to exhibit trans-sialidase activity. SialH catalyzed production of the human milk oligosaccharide 3'-sialyllactose as well as the novel trans-sialylation product 3-sialyllactose using casein glycomacropeptide as sialyl donor and lactose as acceptor. The findings corroborated that Tyr119 and Trp312 in the T. cruzi trans-sialidase are part of an aromatic sandwich structure that confers trans-sialylation activity for lactose sialylation. The in silico identification of trans-glycosidase activity by rational active site topology alignment thus proved to be a quick tool for selecting putative trans-sialidases amongst a large group of glycosyl hydrolases. The approach moreover provided data that help understand structure-function relations of trans-sialidases.
[Mh] Termos MeSH primário: Biologia Computacional
Neuraminidase/metabolismo
[Mh] Termos MeSH secundário: Domínio Catalítico
Glicosilação
Haemophilus/enzimologia
Modelos Moleculares
Neuraminidase/química
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0158434


  4 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27242968
[Au] Autor:Pickering JL; Prosser A; Corscadden KJ; de Gier C; Richmond PC; Zhang G; Thornton RB; Kirkham LA
[Ad] Endereço:Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, The University of Western AustraliaPerth, WA, Australia; School of Paediatrics and Child Health, The University of Western AustraliaPerth, WA, Australia.
[Ti] Título:Haemophilus haemolyticus Interaction with Host Cells Is Different to Nontypeable Haemophilus influenzae and Prevents NTHi Association with Epithelial Cells.
[So] Source:Front Cell Infect Microbiol;6:50, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen that resides in the upper respiratory tract and contributes to a significant burden of respiratory related diseases in children and adults. Haemophilus haemolyticus is a respiratory tract commensal that can be misidentified as NTHi due to high levels of genetic relatedness. There are reports of invasive disease from H. haemolyticus, which further blurs the species boundary with NTHi. To investigate differences in pathogenicity between these species, we optimized an in vitro epithelial cell model to compare the interaction of 10 H. haemolyticus strains with 4 NTHi and 4 H. influenzae-like haemophili. There was inter- and intra-species variability but overall, H. haemolyticus had reduced capacity to attach to and invade nasopharyngeal and bronchoalveolar epithelial cell lines (D562 and A549) within 3 h when compared with NTHi. H. haemolyticus was cytotoxic to both cell lines at 24 h, whereas NTHi was not. Nasopharyngeal epithelium challenged with some H. haemolyticus strains released high levels of inflammatory mediators IL-6 and IL-8, whereas NTHi did not elicit an inflammatory response despite higher levels of cell association and invasion. Furthermore, peripheral blood mononuclear cells stimulated with H. haemolyticus or NTHi released similar and high levels of IL-6, IL-8, IL-10, IL-1ß, and TNFα when compared with unstimulated cells but only NTHi elicited an IFNγ response. Due to the relatedness of H. haemolyticus and NTHi, we hypothesized that H. haemolyticus may compete with NTHi for colonization of the respiratory tract. We observed that in vitro pre-treatment of epithelial cells with H. haemolyticus significantly reduced NTHi attachment, suggesting interference or competition between the two species is possible and warrants further investigation. In conclusion, H. haemolyticus interacts differently with host cells compared to NTHi, with different immunostimulatory and cytotoxic properties. This study provides an in vitro model for further investigation into the pathogenesis of Haemophilus species and the foundation for exploring whether H. haemolyticus can be used to prevent NTHi disease.
[Mh] Termos MeSH primário: Antibiose
Células Epiteliais/microbiologia
Haemophilus/fisiologia
Interações Hospedeiro-Patógeno
[Mh] Termos MeSH secundário: Aderência Bacteriana
Linhagem Celular
Citocinas/secreção
Endocitose
Células Epiteliais/imunologia
Seres Humanos
Leucócitos Mononucleares/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160601
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2016.00050


  5 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27231166
[Au] Autor:Qi CJ; Zhang Q; Yu M; Xu JP; Zheng J; Wang T; Xiao XH
[Ad] Endereço:Department of Endocrinology, Key Laboratory of Endocrinology, Ministry of Health, Peking Union Medical College Hospital, Diabetes Research Center of Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
[Ti] Título:Imbalance of Fecal Microbiota at Newly Diagnosed Type 1 Diabetes in Chinese Children.
[So] Source:Chin Med J (Engl);129(11):1298-304, 2016 Jun 05.
[Is] ISSN:0366-6999
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recent studies have indicated that an imbalance of gut microbiota is associated with the development of type 1 diabetes mellitus (T1DM) and there is no literature regarding it in Chinese children yet. The aim of this study was to evaluate the alteration of gut microbiota between children with newly diagnosed T1DM and healthy controls and to determine if gut microbiota could partly explain the etiology of this disease. METHODS: A case-control study was carried out with 15 children with T1DM and 15 healthy children. The fecal bacteria composition was investigated by high-throughput sequencing of the V3-V4 region of the 16S rDNA gene and analyzed by the estimators of community richness (Chao) indexes. RESULTS: There was a notable lower richness of fecal bacteria in T1DM group than controls (156.53 ± 36.96 vs. 130.0 ± 32.85, P = 0.047). At the genus level, the composition of Blautia was increased in T1DM group than control group whereas the composition of Haemophilus, Lachnospira, Dialister, and Acidaminococcus was decreased. In addition, we found that the percentage of Blautia was correlated positively with HbA1c (ρ = 0.40, P = 0.031), the numbers of T1DM autoantibodies (ρ = 0.42, P = 0.023), and the titers of tyrosine phosphatase autoantibodies (IA-2) (ρ = 0.82, P = 0.000) in the study. CONCLUSIONS: This study showed that gut microbiota was associated with the development of T1DM by affecting the autoimmunity, and the results suggested a potential therapy for T1DM via modulating the gut microbiota.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 1/microbiologia
Fezes/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Autoanticorpos/imunologia
Estudos de Casos e Controles
Criança
Biologia Computacional
Diabetes Mellitus Tipo 1/imunologia
Feminino
Microbioma Gastrointestinal/genética
Microbioma Gastrointestinal/fisiologia
Haemophilus/genética
Haemophilus/isolamento & purificação
Seres Humanos
Masculino
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantibodies); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160528
[St] Status:MEDLINE
[do] DOI:10.4103/0366-6999.182841


  6 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27124204
[Au] Autor:Sharara SL; Tayyar R; Kanafani ZA; Kanj SS
[Ad] Endereço:a School of Medicine, American University of Beirut , Beirut , Lebanon.
[Ti] Título:HACEK endocarditis: a review.
[So] Source:Expert Rev Anti Infect Ther;14(6):539-45, 2016 Jun.
[Is] ISSN:1744-8336
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The HACEK group, referring to Haemophilus spp., Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae, is a rare cause of infective endocarditis (IE). It causes the majority of Gram-negative endocarditis cases and has an excellent prognosis and simple management if properly identified. However, delay in diagnosis and associated complications can render the infection fatal. AREAS COVERED: Over the past few decades, there have been tremendous advancements in understanding the manifestations and progression of HACEK endocarditis (HE). This review tackles the epidemiology of HE, the microbiological characteristics of each organism in the HACEK group, the methods used to diagnose HE, the clinical manifestations, complications, and mortality of patients with HE, as well as the recommended treatment and preventive methods. Expert Commentary: The lack of robust randomized controlled trials in diagnosis and treatment of HE makes it difficult to determine the optimal management of such infections. Nevertheless, advancements in culturing methods have shown progress in isolating and identifying these fastidious organisms. Positive blood cultures for any of the HACEK organisms in the setting of no definite focus of infection is highly suggestive of HE. In such cases, treatment with ceftriaxone or a fluoroquinolone, even without obtaining antibiotic susceptibilities, should be initiated. Moreover, the decision to proceed with surgical intervention should be individualized. As is the case for other IE, HE requires the collaboration of a multidisciplinary team consisting of the infectious disease specialist, cardiologist, cardiothoracic surgeon, and the microbiologist.
[Mh] Termos MeSH primário: Endocardite Bacteriana/microbiologia
Bactérias Gram-Negativas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aggregatibacter/efeitos dos fármacos
Aggregatibacter/isolamento & purificação
Antibacterianos/administração & dosagem
Antibacterianos/uso terapêutico
Cardiobacterium/efeitos dos fármacos
Cardiobacterium/isolamento & purificação
Ecocardiografia
Eikenella corrodens/efeitos dos fármacos
Eikenella corrodens/isolamento & purificação
Endocardite Bacteriana/diagnóstico
Endocardite Bacteriana/tratamento farmacológico
Endocardite Bacteriana/epidemiologia
Bactérias Gram-Negativas/isolamento & purificação
Haemophilus/efeitos dos fármacos
Haemophilus/isolamento & purificação
Seres Humanos
Kingella/efeitos dos fármacos
Kingella/isolamento & purificação
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160429
[St] Status:MEDLINE
[do] DOI:10.1080/14787210.2016.1184085


  7 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27049299
[Au] Autor:Fang R; Wagner BD; Harris JK; Fillon SA
[Ad] Endereço:Department of Biostatistics and Informatics,Colorado School of Public Health,University of Colorado Denver,Aurora,CO,USA.
[Ti] Título:Zero-inflated negative binomial mixed model: an application to two microbial organisms important in oesophagitis.
[So] Source:Epidemiol Infect;144(11):2447-55, 2016 08.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Altered microbial communities are thought to play an important role in eosinophilic oesophagitis, an allergic inflammatory condition of the oesophagus. Identification of the majority of organisms present in human-associated microbial communities is feasible with the advent of high throughput sequencing technology. However, these data consist of non-negative, highly skewed sequence counts with a large proportion of zeros. In addition, hierarchical study designs are often performed with repeated measurements or multiple samples collected from the same subject, thus requiring approaches to account for within-subject variation, yet only a small number of microbiota studies have applied hierarchical regression models. In this paper, we describe and illustrate the use of a hierarchical regression-based approach to evaluate multiple factors for a small number of organisms individually. More specifically, the zero-inflated negative binomial mixed model with random effects in both the count and zero-inflated parts is applied to evaluate associations with disease state while adjusting for potential confounders for two organisms of interest from a study of human microbiota sequence data in oesophagitis.
[Mh] Termos MeSH primário: Esofagite/epidemiologia
Infecções por Fusobacterium/epidemiologia
Fusobacterium/fisiologia
Infecções por Haemophilus/epidemiologia
Haemophilus/fisiologia
[Mh] Termos MeSH secundário: Esofagite/microbiologia
Infecções por Fusobacterium/microbiologia
Infecções por Haemophilus/microbiologia
Seres Humanos
Modelos Estatísticos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160407
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268816000662


  8 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27030383
[Au] Autor:Lim MY; Yoon HS; Rho M; Sung J; Song YM; Lee K; Ko G
[Ad] Endereço:Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea.
[Ti] Título:Analysis of the association between host genetics, smoking, and sputum microbiota in healthy humans.
[So] Source:Sci Rep;6:23745, 2016 Mar 31.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent studies showing clear differences in the airway microbiota between healthy and diseased individuals shed light on the importance of the airway microbiota in health. Here, we report the associations of host genetics and lifestyles such as smoking, alcohol consumption, and physical activity with the composition of the sputum microbiota using 16S rRNA gene sequence data generated from 257 sputum samples of Korean twin-family cohort. By estimating the heritability of each microbial taxon, we found that several taxa, including Providencia and Bacteroides, were significantly influenced by host genetic factors. Smoking had the strongest effect on the overall microbial community structure among the tested lifestyle factors. The abundances of Veillonella and Megasphaera were higher in current-smokers, and increased with the pack-year value and the Fagerstrom Test of Nicotine Dependence (FTND) score. In contrast, Haemophilus decreased with the pack-year of smoking and the FTND score. Co-occurrence network analysis showed that the taxa were clustered according to the direction of associations with smoking, and that the taxa influenced by host genetics were found together. These results demonstrate that the relationships among sputum microbial taxa are closely associated with not only smoking but also host genetics.
[Mh] Termos MeSH primário: Consumo de Bebidas Alcoólicas/genética
Microbiota/genética
Fumar/genética
Escarro/microbiologia
Tabagismo/genética
[Mh] Termos MeSH secundário: Adulto
Bacteroides/classificação
Bacteroides/genética
Exercício/fisiologia
Feminino
Interação Gene-Ambiente
Haemophilus/classificação
Haemophilus/genética
Seres Humanos
Masculino
Megasphaera/classificação
Megasphaera/genética
Meia-Idade
RNA Ribossômico 16S/genética
Tabagismo/microbiologia
Veillonella/classificação
Veillonella/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160401
[St] Status:MEDLINE
[do] DOI:10.1038/srep23745


  9 / 2226 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26961389
[Au] Autor:Wang K; Lu W; Tu Q; Ge Y; He J; Zhou Y; Gou Y; Van Nostrand JD; Qin Y; Li J; Zhou J; Li Y; Xiao L; Zhou X
[Ad] Endereço:State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
[Ti] Título:Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus.
[So] Source:Sci Rep;6:22943, 2016 Mar 10.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Several studies have explored the origin and development mechanism of oral lichen planus (OLP) with limited attention to the role of bacteria in the progression of this common oral disease. Here we utilized MiSeq sequencing of 16S rRNA gene amplicons to identify complex oral microbiota associated with OLP from saliva samples of two subtypes (reticular and erosive) of OLP patients and healthy controls. Our analyses indicated that the overall structure of the salivary microbiome was not significantly affected by disease status. However, we did observe evident variations in abundance for several taxonomic groups in OLP. Porphyromonas and Solobacterium showed significantly higher relative abundances, whereas Haemophilus, Corynebacterium, Cellulosimicrobium and Campylobacter showed lower abundances in OLP patients, as compared with healthy controls. In addition, we explored specific microbial co-occurrence patterns in OLP, and revealed significantly fewer linkers of Streptococcus comprising species in erosive OLP. Furthermore, the disease severity and immune dysregulation were also genus-associated, including with Porphyromonas that correlated to disease scores and salivary levels of interleukin (IL)-17 and IL-23. Overall, this study provides a general description of oral microbiome in OLP, and it will be useful for further investigation of their potential roles in the initiation and immune modulation of OLP.
[Mh] Termos MeSH primário: Líquen Plano Bucal/genética
Microbiota/genética
RNA Ribossômico 16S/genética
Saliva/microbiologia
[Mh] Termos MeSH secundário: Adulto
Campylobacter/genética
Campylobacter/isolamento & purificação
Campylobacter/patogenicidade
Corynebacterium/genética
Corynebacterium/isolamento & purificação
Corynebacterium/patogenicidade
Feminino
Haemophilus/genética
Haemophilus/isolamento & purificação
Haemophilus/patogenicidade
Seres Humanos
Líquen Plano Bucal/microbiologia
Líquen Plano Bucal/patologia
Masculino
Meia-Idade
Porphyromonas/genética
Porphyromonas/isolamento & purificação
Porphyromonas/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160311
[St] Status:MEDLINE
[do] DOI:10.1038/srep22943


  10 / 2226 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
[PMID]:26912163
[Au] Autor:Kosikowska U; Biernasiuk A; Korona-Glowniak I; Kiciak S; Tomasiewicz K; Malm A
[Ad] Endereço:Chair and Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics, Medical University of Lublin, Lublin, Poland.
[Ti] Título:The Association of Chronic Hepatitis C with Respiratory Microbiota Disturbance on the Basis of Decreased Haemophilus Spp. Colonization.
[So] Source:Med Sci Monit;22:625-32, 2016 Feb 25.
[Is] ISSN:1643-3750
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND Haemophilus species are the most common microbiota in humans. The aim of this paper was to investigate Haemophilus spp., mainly H. parainfluenzae prevalence, in the upper respiratory tract of chronic hepatitis C (CHC-positive) patients with or without therapy using pegylated interferon alfa and ribavirin. MATERIAL AND METHODS We collected 462 samples from 54 healthy people and 100 CHC-positive patients at various stages: before (group A), during (group B), and after (group C) antiviral therapy. Identification of bacterial isolates including biotypes and antimicrobials susceptibility was accomplished by means of standard microbiological methods. RESULTS In 70.4% of healthy people (control group) and in 27.0% of CHC-positive patients, the presence of haemophili, mainly H. parainfluenzae was observed, and those differences were statistically significant (p<0.0001). Statistically significant differences in Haemophilus spp. colonization were also observed among healthy people and CHC-positive patients from group A (p=0.0012) and from B or C groups (p<0.0001). Resistance to ampicillin in beta-lactamase-positive isolates and multidrug resistance (MDR) of H. parainfluenzae was detected mainly in group A. CONCLUSIONS The obtained data suggest that chronic hepatitis C, together with antiviral therapy, may influence the respiratory tract microbiota composition as found using haemophili, mainly H. parainfluenzae.
[Mh] Termos MeSH primário: Haemophilus/fisiologia
Hepatite C Crônica/microbiologia
Microbiota
Sistema Respiratório/microbiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Candida/fisiologia
Contagem de Colônia Microbiana
Resistência Microbiana a Medicamentos
Haemophilus/isolamento & purificação
Seres Humanos
Meia-Idade
Nasofaringe/microbiologia
Sistema Respiratório/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE



página 1 de 223 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde