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Pesquisa : B03.440.450.900.500 [Categoria DeCS]
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[PMID]:27697559
[Au] Autor:Siva VS; Wang L; Qiu L; Zhou Z; Liu C; Yang J; Yang C; Song L
[Ad] Endereço:Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Centre for Climate Change St
[Ti] Título:Polymorphism in a serine protease inhibitor gene and its association with the resistance of bay scallop (Argopecten irradians) to Listonella anguillarum challenge.
[So] Source:Fish Shellfish Immunol;59:1-8, 2016 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Serine protease inhibitors (SPIs) play a crucial role in regulation of both host and bacterial serine protease. They are classified into several protein families, where Kazal-type inhibitors are one of families with multi-domain. In the present study, the polymorphism of AiSPI from Bay scallop Argopecten irradians was found to be associated with disease resistance of bay scallop against Listonella anguillarum. Nine single nucleotide polymorphisms (SNPs) were identified in the exon region of AiSPI, where five SNPs were non-synonymous mutation. Three of these mutations were located in "kazal-like 3"domain, two SNP loci positioned at +536, +1312 were selected for further association studies. For the locus +536, the genotype frequency of A/G in the resistant stock (12.8%) was significantly lower (p < 0.05) than that in the susceptible stock (35.1%), while, the genotype A/A in the resistant stock (87.2%) was significantly higher in comparison with susceptible stock (64.9%) (p < 0.05). The G allele frequencies were 6.4% and 17.6% in resistant stock and susceptible stock, respectively, and χ -test revealed a significant difference in the frequency distribution between the two stocks (p < 0.05). But there was no significant association between the mutation C-T at locus +1312 with either resistant or susceptible group (p > 0.05). The genotype frequencies of T/T, T/C, C/C at locus +1312 were 94.6%, 2.7% and 2.7% respectively in the susceptible stock, while 100%, 0% and 0% respectively in the resistant stock. The amino acid change for the mutation at locus +536 A-G was from asparagine to serine, and the predicted homology model of this amino acid variation could affect its function as well as the structural integrity of the domain. In vitro elastase inhibition assay of the protein variants at locus +536 was conducted to explicate the effect of SNP. The increasing concentration of protein (0 mmol/L- 2.93 mmol/L) was incubated with 80 nmol/L elastase where the residual enzyme activity values for rAiSPI (N) with A variant and rAiSPI (S) with G variant were started to reduce from 0.40 to 0.215 and 0.435 to 0.356, respectively. The elastase inhibition ability of rAiSPI (N) variant was significantly higher than that of rAiSPI (S) (p < 0.01). The results suggested that the mutation at locus +536A/A significantly associated with disease resistance of bay scallop would shed light for selective breeding program.
[Mh] Termos MeSH primário: Imunidade Inata/genética
Listonella/fisiologia
Pectinidae/genética
Pectinidae/imunologia
Polimorfismo de Nucleotídeo Único
Inibidores de Serino Proteinase/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Listonella/imunologia
Mutação
Pectinidae/microbiologia
Inibidores de Serino Proteinase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Serine Proteinase Inhibitors)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:26382599
[Au] Autor:Altinok I; Capkin E; Karsi A
[Ad] Endereço:Department of Fisheries Technology Engineering, Faculty of Marine Science, Karadeniz Technical University, Trabzon 61530, Turkey. Electronic address: ialtinok@ktu.edu.tr.
[Ti] Título:Succinate dehydrogenase mutant of Listonella anguillarum protects rainbow trout against vibriosis.
[So] Source:Vaccine;33(42):5572-5577, 2015 Oct 13.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Listonella anguillarum is a Gram-negative facultative anaerobic rod causing hemorrhagic septicemia in marine and rarely in freshwater fish. Succinate dehydrogenase (SDH) plays an important role in the tricarboxylic acid (TCA) cycle by oxidizing succinate to fumarate while reducing ubiquinone to ubiquinol. Recent studies indicate that central metabolic pathways, including the TCA cycle, contribute to bacterial virulence. However, the role of SDH in L. anguillarum virulence has not been studied. Here, we report in-frame deletion of the succinate dehydrogenase iron-sulfur protein (SDHB) and its role in L. anguillarum virulence in rainbow trout. To accomplish this goal, upstream and downstream regions of the L. anguillarum sdhB gene were amplified in-frame and cloned into a suicide plasmid. The chromosomal sdhB gene of L. anguillarum was deleted by homologous recombination. Virulence and immunogenicity of the L. anguillarum ΔsdhB mutant (LaΔsdhB) were determined in rainbow trout. Results show that LaΔsdhB was highly attenuated in rainbow trout, and fish immunized with LaΔsdhB displayed high relative survival rate after exposure to wild type L. anguillarum. These findings indicate SDH is important in L. anguillarum virulence in rainbow trout, and LaΔsdhB could be used as an immersion, oral, or injection vaccine to protect rainbow trout against vibriosis.
[Mh] Termos MeSH primário: Vacinas Bacterianas/imunologia
Doenças dos Peixes/prevenção & controle
Listonella/enzimologia
Oncorhynchus mykiss/imunologia
Succinato Desidrogenase/genética
Vibrioses/veterinária
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas com Ferro-Enxofre/genética
Listonella/genética
Listonella/patogenicidade
Oncorhynchus mykiss/microbiologia
Deleção de Sequência
Vacinas Atenuadas/imunologia
Vibrioses/prevenção & controle
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Vaccines); 0 (Iron-Sulfur Proteins); 0 (Vaccines, Attenuated); EC 1.3.99.1 (Succinate Dehydrogenase)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171021
[Lr] Data última revisão:
171021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150919
[St] Status:MEDLINE


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[PMID]:25970236
[Au] Autor:Arkoosh MR; Dietrich JP
[Ad] Endereço:a National Oceanic and Atmospheric Administration, National Marine Fisheries Service, Northwest Fisheries Science Center , Environmental and Fisheries Sciences Division , 2032 Southeast OSU Drive, Newport , Oregon 97365 , USA.
[Ti] Título:Pathogenicity of members of the vibrionaceae family to cultured juvenile sablefish.
[So] Source:J Aquat Anim Health;27(2):96-103, 2015 Jun.
[Is] ISSN:0899-7659
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sablefish Anoplopoma fimbria are a prized seafood species due to their high oil content and white flaky flesh. Raising these species in culture can help to provide an important source of protein for humans and relief to declining wild fish populations. Understanding the environmental factors that influence the production of Sablefish is important for successful culturing. The significance of host-pathogen interactions in Sablefish culture and the resulting environmental implications are unknown. Pathogens could potentially cause losses of cultured Sablefish stocks due to disease, while Sablefish cultured in net pens may also serve as reservoirs for pathogens and potentially transmit disease to wild fish species. In this initial study, the susceptibility of juvenile Sablefish to three bacterial pathogens from the family Vibrionaceae was examined. Listonella anguillarum, Vibrio ordalii, and V. splendidus can pose serious economic threats to cultured fish and shellfish. Groups of juvenile Sablefish were exposed to five concentrations of each of the pathogens. Sablefish were susceptible to L. anguillarum, but were resistant to V. ordalii and V. splendidus at exposure concentrations of ≤ 1.32 × 107 CFU/mL and ≤ 3.57 × 106 CFU/mL, respectively. The greatest L. anguillarum concentration examined (8.7 × 106 CFU/mL) resulted in 24% mortality in juvenile Sablefish. A 24% loss of Sablefish stock could significantly influence an aquaculture program. As determined by multiple logistic regression, the survival of Sablefish to L. anguillarum exposure was significantly affected by their body mass, and larger fish had a greater probability of survival. Aquaculture operations could employ various strategies to minimize the loss of juvenile Sablefish by accounting for their size and known susceptibilities to pathogens.
[Mh] Termos MeSH primário: Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Listonella/patogenicidade
Perciformes
Vibrio/patogenicidade
[Mh] Termos MeSH secundário: Animais
Aquicultura
Doenças dos Peixes/patologia
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/patologia
Análise de Sobrevida
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150514
[Lr] Data última revisão:
150514
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150514
[St] Status:MEDLINE
[do] DOI:10.1080/08997659.2015.1019159


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[PMID]:25938634
[Au] Autor:Arkoosh MR; Van Gaest AL; Strickland SA; Hutchinson GP; Krupkin AB; Dietrich JP
[Ti] Título:Dietary Exposure to Individual Polybrominated Diphenyl Ether Congeners BDE-47 and BDE-99 Alters Innate Immunity and Disease Susceptibility in Juvenile Chinook Salmon.
[So] Source:Environ Sci Technol;49(11):6974-81, 2015 Jun 02.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polybrominated diphenyl ethers (PBDEs), used as commercial flame-retardants, are bioaccumulating in threatened Pacific salmon. However, little is known of PBDE effects on critical physiological functions required for optimal health and survival. BDE-47 and BDE-99 are the predominant PBDE congeners found in Chinook salmon collected from the Pacific Northwest. In the present study, both innate immunity (phagocytosis and production of superoxide anion) and pathogen challenge were used to evaluate health and survival in groups of juvenile Chinook salmon exposed orally to either BDE-47 or BDE-99 at environmentally relevant concentrations. Head kidney macrophages from Chinook salmon exposed to BDE-99, but not those exposed to BDE-47, were found to have a reduced ability in vitro to engulf foreign particles. However, both congeners increased the in vitro production of superoxide anion in head kidney macrophages. Salmon exposed to either congener had reduced survival during challenge with the pathogenic marine bacteria Listonella anguillarum. The concentration response curves generated for these end points were nonmonotonic and demonstrated a requirement for using multiple environmentally relevant PBDE concentrations for effect studies. Consequently, predicting risk from toxicity reference values traditionally generated with monotonic concentration responses may underestimate PBDE effect on critical physiological functions required for optimal health and survival in salmon.
[Mh] Termos MeSH primário: Dieta
Suscetibilidade a Doenças/imunologia
Exposição Ambiental/análise
Éteres Difenil Halogenados/toxicidade
Imunidade Inata/efeitos dos fármacos
Salmão/imunologia
[Mh] Termos MeSH secundário: Animais
Listonella/efeitos dos fármacos
Listonella/fisiologia
Probabilidade
Salmão/microbiologia
Análise de Sobrevida
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2,2',4,4',5-brominated diphenyl ether); 0 (Halogenated Diphenyl Ethers); 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150602
[Lr] Data última revisão:
150602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150505
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.5b01076


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[PMID]:25458310
[Au] Autor:Lu XJ; Chen Q; Yang GJ; Chen J
[Ad] Endereço:Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China
[Ti] Título:The TNFα converting enzyme (TACE) from ayu (Plecoglossus altivelis) exhibits TNFα shedding activity.
[So] Source:Mol Immunol;63(2):497-504, 2015 Feb.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:TNFα converting enzyme (TACE) is responsible for converting membrane-anchored TNFα to its soluble form in mammalian. However, the function and characteristics of TACE in teleosts is unclear. In this study, we report the cloning of a cDNA sequence of the PaTACE from ayu, Plecoglossus altivelis. PaTACE encodes an 865-aa polypeptide, which is closest to the TACE gene found in pufferfish (Takifugu rubripes). PaTACE mRNA was detected in all the tissues tested, although it was considerably higher in liver, spleen, and brain tissues following infection with Listonella anguillarum. The recombinant region including the PaTACE catalytic domain was used to produce anti-PaTACE IgG. Western blot results revealed two bands for PaTACE from monocytes/macrophages. PNGase F digestion confirmed that the high molecular mass of PaTACE was caused by glycosylation. TACE activity in cell homogenates from ayu monocytes/macrophages increased following L. anguillarum infection. Moreover, PaTACE neutralization led to downregulation of TNFα expression in the supernatant of ayu monocyte/macrophages. Anti-PaTACE IgG also decreased respiratory burst in monocytes/macrophages. In conclusion, we report for the first time the TNFα-converting activity of TACE from a teleost. More investigation is needed to illustrate PaTACE-shedding activity in other immune regulators.
[Mh] Termos MeSH primário: Proteínas ADAM/metabolismo
Osmeriformes/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Proteínas ADAM/química
Proteínas ADAM/genética
Proteínas ADAM/imunologia
Proteína ADAM17
Animais
Anticorpos/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Listonella/efeitos dos fármacos
Listonella/fisiologia
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Dados de Sequência Molecular
Monócitos/efeitos dos fármacos
Monócitos/metabolismo
Osmeriformes/imunologia
Osmeriformes/microbiologia
Filogenia
Estrutura Terciária de Proteína
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Explosão Respiratória/efeitos dos fármacos
Alinhamento de Sequência
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies); 0 (RNA, Messenger); 0 (Tumor Necrosis Factor-alpha); EC 3.4.24.- (ADAM Proteins); EC 3.4.24.86 (ADAM17 Protein)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25449372
[Au] Autor:Wei X; Xu J; Yang J; Liu X; Zhang R; Wang W; Yang J
[Ad] Endereço:Shandong Provincial Key Laboratory of Marine Ecology Restoration, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China.
[Ti] Título:Involvement of a Serpin serine protease inhibitor (OoSerpin) from mollusc Octopus ocellatus in antibacterial response.
[So] Source:Fish Shellfish Immunol;42(1):79-87, 2015 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Serpin is an important member of serine protease inhibitors (SPIs), which is capable of regulating proteolytic events and involving in a variety of physiological processes. In present study, a Serpin homolog was identified from Octopus ocellatus (designated as OoSerpin). Full-length cDNA of OoSerpin was of 1735 bp, containing a 5' untranslated region of 214 bp, a 3' UTR of 282 bp, and an open reading frame of 1239 bp. The open reading frame encoded a polypeptide of 412 amino acids which has a predicted molecular weight of 46.5 kDa and an isoelectric point of 8.52. The OoSerpin protein shares 37% sequence identity with other Serpins from Mus musculus (NP_941373) and Ixodes scapularis (XP_002407493). The existence of a conserved SERPIN domain strongly suggested that OoSerpin was a member of the Serpin subfamily. Expression patterns of OoSerpin, both in tissues and towards bacterial stimulation, were then characterized. The mRNA of OoSerpin was constitutively expressed at different levels in all tested tissues of untreated O. ocellatus, including mantle (lowest), muscle, renal sac, gill, hemocyte, gonad, systemic heart, and hepatopancreas (highest). The transcriptional level of OoSerpin was significantly up-regulated (P<0.01) in O. ocellatus upon bacterial challenges with Vibrio anguillarum and Micrococcus luteus, indicating its involvement in the antibacterial immune response. Furthermore, rOoSerpin, the recombinant protein of OoSerpin, exhibited strong abilities to inhibit proteinase activities of trypsin and chymotrypsin as well as the growth of Escherichia coli. Our results demonstrate that OoSerpin is a potential antibacterial factor involved in the immune response of O. ocellatus against bacterial infection.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica/imunologia
Listonella/imunologia
Micrococcus luteus/imunologia
Octopodiformes/genética
Octopodiformes/imunologia
Serpinas/genética
Serpinas/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Biologia Computacional
Primers do DNA/genética
DNA Complementar/genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/crescimento & desenvolvimento
Componentes do Gene
Perfilação da Expressão Gênica
Dados de Sequência Molecular
Octopodiformes/microbiologia
Fases de Leitura Aberta/genética
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
Serpinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Complementary); 0 (Serpins)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141208
[Lr] Data última revisão:
141208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25454083
[Au] Autor:Mosca F; Ciulli S; Volpatti D; Romano N; Volpe E; Bulfon C; Massimini M; Caccia E; Galeotti M; Tiscar PG
[Ad] Endereço:Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
[Ti] Título:Defensive response of European sea bass (Dicentrarchus labrax) against Listonella anguillarum or Photobacterium damselae subsp. piscicida experimental infection.
[So] Source:Vet Immunol Immunopathol;162(3-4):83-95, 2014 Dec 15.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sea bass were experimentally infected with Listonella anguillarum or Photobacterium damselae subsp. piscicida (Phdp). At 24 and 72h post-infection, the expression analysis of immune-relevant genes (IL-1ß, IL-6, IL-8, Hepcidin), the transcriptional level and detection of HSP70, and the quantification of serum iron were investigated in association with the histological analysis and the bacterial recognition in tissues by immunohistochemistry. At 15 days post-infection, the specific antibody response was detected in surviving fish, as well as the transcriptional levels of TcR and BcR sequences. Both experimental infections were characterized by a similar acute response, whereas different histological and immunohistochemistry evidences were observed. In particular, the early reaction appeared suitable for the clearance of L. anguillarum, thus limiting the histological lesions, the bacterial dissemination and the further development of acquired immunity in surviving fish. On the contrary, the innate response appeared not enough to resolve the Phdp infection, which was characterized by tissue damage, bacterial widespread and substantial detection of specific humoral immunity in surviving fish, also associated to lymphocytes clonal expansion. Besides the opportunistic conditions involved in fish vibriosis and pasteurellosis, the comparison between these experimental infection models seems to suggest that the rate of development of the acquired immunity is strictly linked to the activation of the host innate response combined to the degree of bacterial virulence.
[Mh] Termos MeSH primário: Bass
Doenças dos Peixes/microbiologia
Regulação Bacteriana da Expressão Gênica/imunologia
Infecções por Bactérias Gram-Negativas/veterinária
Listonella/imunologia
Photobacterium/imunologia
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/imunologia
Infecções por Bactérias Gram-Negativas/imunologia
Infecções por Bactérias Gram-Negativas/microbiologia
Proteínas de Choque Térmico HSP70/genética
Proteínas de Choque Térmico HSP70/imunologia
Rim Cefálico/imunologia
Hepcidinas/genética
Hepcidinas/imunologia
Imuno-Histoquímica/veterinária
Interleucinas/genética
Interleucinas/imunologia
RNA/química
RNA/genética
Distribuição Aleatória
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Baço/imunologia
Timo/imunologia
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (HSP70 Heat-Shock Proteins); 0 (Hepcidins); 0 (Interleukins); 0 (Tumor Necrosis Factor-alpha); 63231-63-0 (RNA)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141205
[Lr] Data última revisão:
141205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25010728
[Au] Autor:Chu CQ; Lu XJ; Li CH; Chen J
[Ad] Endereço:Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
[Ti] Título:Molecular characterization of a CXCL8-like protein from ayu and its effect on chemotaxis of neutrophils and monocytes/macrophages.
[So] Source:Gene;548(1):48-55, 2014 Sep 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:CXCL8, a CXC-type chemokine, plays a crucial role in acute inflammation by recruiting and mediating neutrophils and other cells. In this study, the cDNA and genomic DNA sequence of a CXCL8-like protein (PaCXCL8l) from ayu (Plecoglossus altivelis) was determined. Sequence analysis showed that PaCXCL8l represented the typical structure of animal CXCL8s. Phylogenetic tree analysis indicated that PaCXCL8l was closest to CXCL8 of Atlantic cod (Gadus morhua). Constitutive expression of PaCXCL8l was detected in all tested tissues and monocytes/macrophages, and its expression dramatically increased upon Listonella anguillarum infection. In vitro, recombinant PaCXCL8l exhibited a significant chemotactic effect on neutrophils at 0.1 µg/ml and on monocytes/macrophages at 1.0 µg/ml. In vivo, the numbers of peritoneal neutrophils and monocytes/macrophages were both up-regulated following intraperitoneal administration of recombinant PaCXCL8l. These results suggest that PaCXCL8l is crucially involved in the immune response of ayu by mediating chemotaxis of neutrophils and monocytes/macrophages.
[Mh] Termos MeSH primário: Quimiotaxia
Proteínas de Peixes/genética
Interleucina-8/genética
Neutrófilos/fisiologia
Osmeriformes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Células Cultivadas
Clonagem Molecular
Doenças dos Peixes/patologia
Proteínas de Peixes/metabolismo
Regulação da Expressão Gênica
Infecções por Bactérias Gram-Negativas/patologia
Infecções por Bactérias Gram-Negativas/veterinária
Interações Hospedeiro-Patógeno/genética
Interleucina-8/metabolismo
Listonella/patogenicidade
Macrófagos/metabolismo
Macrófagos/microbiologia
Dados de Sequência Molecular
Monócitos/fisiologia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Interleukin-8)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140726
[Lr] Data última revisão:
140726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140711
[St] Status:MEDLINE


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[PMID]:24594008
[Au] Autor:Lu XJ; Chu CQ; Chen Q; Chen J
[Ad] Endereço:Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
[Ti] Título:A novel lipopolysaccharide-binding protein (LBP) gene from sweetfish Plecoglossus altivelis: molecular characterization and its role in the immune response of monocytes/macrophages.
[So] Source:Fish Shellfish Immunol;38(1):111-8, 2014 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lipopolysaccharide-binding protein (LBP) belongs to the lipid transfer/LBP (LT-LBP) family, and plays a crucial role in the recognition of bacterial components that modulate cellular signals in phagocytic cells. Although several LBPs have been identified in teleosts, the effects of LBP homologs on teleost phagocytic cells are still obscure. Here, we report the cloning a novel full-length cDNA sequence of LBP-like protein (paLBP) gene from sweetfish, Plecoglossus altivelis. The paLBP cDNA encoded a 464 aa polypeptide, which was closest to that of rainbow smelt (Osmerus mordax). paLBP mRNA was detected mainly in the spleen, liver, and head kidney and levels dramatically increased in various tissues after Listonella anguillarum infection. In contrast to mammalian studies, paLBP mRNA could also be detected in sweetfish monocytes/macrophages. Recombinant paLBP showed LPS-binding activity and Western blot results revealed a significant increase of paLBP in the supernatant of sweetfish monocytes/macrophages challenged with L. anguillarum. Moreover, paLBP neutralization led to up-regulation of IL-1ß and TNF-α mRNA as well as respiratory burst activity in sweetfish monocytes/macrophages in response to L. anguillarum or LPS challenge. Therefore, these results suggest that paLBP is an inducible acute-phase protein mediating the immune response of sweetfish monocytes/macrophages upon bacterial challenge.
[Mh] Termos MeSH primário: Proteínas da Fase Aguda/metabolismo
Proteínas de Transporte/metabolismo
Imunidade Celular/fisiologia
Macrófagos/fisiologia
Glicoproteínas de Membrana/metabolismo
Monócitos/fisiologia
Osmeriformes/metabolismo
[Mh] Termos MeSH secundário: Proteínas da Fase Aguda/genética
Sequência de Aminoácidos
Animais
Proteínas de Transporte/genética
Regulação da Expressão Gênica/imunologia
Infecções por Bactérias Gram-Negativas/imunologia
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Listonella
Glicoproteínas de Membrana/genética
Dados de Sequência Molecular
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acute-Phase Proteins); 0 (Carrier Proteins); 0 (Membrane Glycoproteins); 0 (RNA, Messenger); 0 (lipopolysaccharide-binding protein)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140428
[Lr] Data última revisão:
140428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140306
[St] Status:MEDLINE


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[PMID]:24213925
[Au] Autor:Mu C; Chen L; Zhao J; Wang C
[Ad] Endereço:School of Marine Science of Ningbo University, Ningbo, 315211, People's Republic of China.
[Ti] Título:Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum.
[So] Source:Mol Biol Rep;41(1):139-44, 2014 Jan.
[Is] ISSN:1573-4978
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97- and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.
[Mh] Termos MeSH primário: Bivalves/genética
Lectinas Tipo C/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Bivalves/imunologia
Bivalves/metabolismo
Bivalves/microbiologia
Clonagem Molecular
Expressão Gênica
Regulação da Expressão Gênica/imunologia
Hemócitos/imunologia
Hemócitos/metabolismo
Hemócitos/microbiologia
Interações Hospedeiro-Patógeno
Lectinas Tipo C/metabolismo
Listonella/fisiologia
Dados de Sequência Molecular
Especificidade de Órgãos
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lectins, C-Type)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131112
[St] Status:MEDLINE
[do] DOI:10.1007/s11033-013-2846-2



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