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  1 / 7213 MEDLINE  
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[PMID]:29335469
[Au] Autor:Aunkham A; Zahn M; Kesireddy A; Pothula KR; Schulte A; Baslé A; Kleinekathöfer U; Suginta W; van den Berg B
[Ad] Endereço:Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
[Ti] Título:Structural basis for chitin acquisition by marine Vibrio species.
[So] Source:Nat Commun;9(1):220, 2018 01 15.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chitin, an insoluble polymer of N-acetylglucosamine, is one of the most abundant biopolymers on Earth. By degrading chitin, chitinolytic bacteria such as Vibrio harveyi are critical for chitin recycling and maintenance of carbon and nitrogen cycles in the world's oceans. A decisive step in chitin degradation is the uptake of chito-oligosaccharides by an outer membrane protein channel named chitoporin (ChiP). Here, we report X-ray crystal structures of ChiP from V. harveyi in the presence and absence of chito-oligosaccharides. Structures without bound sugar reveal a trimeric assembly with an unprecedented closing of the transport pore by the N-terminus of a neighboring subunit. Substrate binding ejects the pore plug to open the transport channel. Together with molecular dynamics simulations, electrophysiology and in vitro transport assays our data provide an explanation for the exceptional affinity of ChiP for chito-oligosaccharides and point to an important role of the N-terminal gate in substrate transport.
[Mh] Termos MeSH primário: Carbono/metabolismo
Quitina/metabolismo
Nitrogênio/metabolismo
Vibrio/metabolismo
[Mh] Termos MeSH secundário: Acetilglucosamina/metabolismo
Proteínas da Membrana Bacteriana Externa/química
Proteínas da Membrana Bacteriana Externa/genética
Proteínas da Membrana Bacteriana Externa/metabolismo
Ciclo do Carbono
Cristalografia por Raios X
Modelos Moleculares
Ciclo do Nitrogênio
Oceanos e Mares
Oligossacarídeos/metabolismo
Porinas/química
Porinas/genética
Porinas/metabolismo
Conformação Proteica
Água do Mar/química
Água do Mar/microbiologia
Vibrio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Oligosaccharides); 0 (Porins); 1398-61-4 (Chitin); 7440-44-0 (Carbon); N762921K75 (Nitrogen); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02523-y


  2 / 7213 MEDLINE  
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[PMID]:29284014
[Au] Autor:Katharios P; Kalatzis PG; Kokkari C; Sarropoulou E; Middelboe M
[Ad] Endereço:Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Crete, Greece.
[Ti] Título:Isolation and characterization of a N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves.
[So] Source:PLoS One;12(12):e0190083, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from a sea-cage aquaculture farm in Greece, and characterized using microbiological methods and genomic analysis. Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non-contractile tail. The phage had a narrow host range infecting only the bacterial host, a latent period of 30 min and a burst size of 24 virions per infected bacterium. Its genome size was 78,145 bp and genomic analysis identified 107 densely-packed genes, 40 of which could be annotated. In addition to the very large virion encapsulated DNA-dependent RNA polymerase which is the signature of the N4-like genus, an interesting feature of the novel phage is the presence of a self-splicing group I intron in the thymidylate synthase gene. A tRNAStop interrupted by a ~2.5kb open reading frame-containing area was also identified. The absence of genes related to lysogeny along with the high efficacy observed during in vitro cell lysis trials, indicate that the vB_VspP_pVa5 is a potential candidate component in a bacteriophage cocktail suitable for the biological control of V. splendidus in aquaculture.
[Mh] Termos MeSH primário: Bacteriófagos/isolamento & purificação
Bivalves/microbiologia
Peixes/microbiologia
Vibrio/virologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Microscopia Eletrônica de Transmissão
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190083


  3 / 7213 MEDLINE  
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[PMID]:29231719
[Au] Autor:Naik SP; Scholin J; Ching S; Chi F; Herpfer M
[Ad] Endereço:Innovation Center, Oil-Dri Corporation of America , 777 Forest Edge Road, Vernon Hills, Illinois 60061-3197, United States.
[Ti] Título:Quorum Sensing Disruption in Vibrio harveyi Bacteria by Clay Materials.
[So] Source:J Agric Food Chem;66(1):40-44, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This work describes the use of clay minerals as catalysts for the degradation of quorum sensing molecule N-(3-oxooctanoyl)-dl-homoserine lactone. Certain clay minerals as a result of their surface properties and porosity can catalytically degrade the quorum sensing molecule into smaller fragments. The disruption of quorum sensing by clay in a growing Gram-negative Vibrio harveyi bacteria culture was also studied by monitoring luminescence and population density of the bacteria, wherein quenching of bacterial quorum sensing activity was observed by means of luminescence reduction. The results of this study show that food-grade clays can be used as biocatalysts in disrupting bacterial activity in various media.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Silicatos de Alumínio/química
Silicatos de Alumínio/farmacologia
Vibrio/efeitos dos fármacos
[Mh] Termos MeSH secundário: 4-Butirolactona/química
4-Butirolactona/metabolismo
Antibacterianos/química
Antibacterianos/farmacologia
Medições Luminescentes
Percepção de Quorum/efeitos dos fármacos
Percepção de Quorum/fisiologia
Vibrio/metabolismo
Vibrio/fisiologia
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Anti-Bacterial Agents); 0 (N-(3-oxooctanoyl)homoserine lactone); 1302-87-0 (clay); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03918


  4 / 7213 MEDLINE  
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[PMID]:29101486
[Au] Autor:Sunil Kumar D; Janakiram P; Murali Krishna Kumar M; Krishna Geetha G
[Ad] Endereço:Department of Marine Living Resources, Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India.
[Ti] Título:Inhibitory activity of bio-active compounds isolated from Anadara granosa in shrimp health management.
[So] Source:World J Microbiol Biotechnol;33(11):207, 2017 Nov 03.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The crude extract isolated from the visceral mass of Anadara granosa, an intertidal bivalve mollusc was tested for inhibitory activity against pathogenic bacteria of the shrimp and fish viz. Vibrio harveyi and Staphylococcus aureus respectively by agar well diffusion and contact bioautography methods. Maximum inhibitory activity was shown against V. harveyi by methanol and chloroform (9:1) extract. Twelve fractions (1-12) could be separated from the crude extract through column chromatography. Five out of twelve fractions (7, 8, 9, 10, and 11) showed antibacterial activity and they were further run on column chromatography for purity. The fraction no. 9 showed highest antibacterial activity among the five and was subjected to NMR for the proton, C and H -H correlation, IR and mass spectral analysis for structural elucidation. Structure of the compound isolated from fraction no: 9 was determined as 1-(((2Z, 4Z)-dodeca-2,4-dienoyl)oxy)-3-hydroxypropan-2-yl tetradecanoate.
[Mh] Termos MeSH primário: Antibacterianos/química
Antibacterianos/farmacologia
Arcidae/química
Artemia/microbiologia
Fatores Biológicos/química
Fatores Biológicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Metanol/química
Testes de Sensibilidade Microbiana
Staphylococcus aureus/efeitos dos fármacos
Vibrio/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biological Factors); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171114
[Lr] Data última revisão:
171114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171105
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2340-4


  5 / 7213 MEDLINE  
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[PMID]:28887768
[Au] Autor:Fan G; Bressloff PC
[Ad] Endereço:Department of Mathematics, University of Utah, Salt Lake City, UT, 84112, USA.
[Ti] Título:Population Model of Quorum Sensing with Multiple Parallel Pathways.
[So] Source:Bull Math Biol;79(11):2599-2626, 2017 Nov.
[Is] ISSN:1522-9602
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quorum sensing (QS) is a bacterial communication mechanism that uses signal-receptor binding to regulate gene expression based on cell density, resulting in group behaviors such as biofilm formation, bioluminescence and stress response. In certain bacterial species such as Vibrio harveyi, several parallel QS signaling pathways drive a single phosphorylation-dephosphorylation cycle, which in turn regulates QS target genes. In this paper, we investigate the possible role of parallel signaling pathways by developing a mathematical model of QS in V. harveyi at both the single-cell and population levels. First we explore how signal integration may be achieved at the single-cell level, and how different model parameters influence the process. We then consider two examples of signal integration at the population level: a one-population model responding to two environmental cues (cell density and mass transfer), and a two-population model with distinct cell densities. In each case, we use contraction analysis to reduce the population model to an effective single-cell model.
[Mh] Termos MeSH primário: Modelos Biológicos
Percepção de Quorum/fisiologia
[Mh] Termos MeSH secundário: Simulação por Computador
Conceitos Matemáticos
Transdução de Sinais
Análise de Célula Única
Vibrio/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE
[do] DOI:10.1007/s11538-017-0343-9


  6 / 7213 MEDLINE  
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[PMID]:28857026
[Au] Autor:Lucena T; Arahal DR; Ruvira MA; Navarro-Torre S; Mesa J; Pajuelo E; Rodriguez-Llorente ID; Rodrigo-Torres L; Piñar MJ; Pujalte MJ
[Ad] Endereço:1​Departamento de Microbiología y Ecología & Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain.
[Ti] Título:Vibrio palustris sp. nov. and Vibrio spartinae sp. nov., two novel members of the Gazogenes clade, isolated from salt-marsh plants (Arthrocnemum macrostachyum and Spartina maritima).
[So] Source:Int J Syst Evol Microbiol;67(9):3506-3512, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two bacterial strains, EAod9T and SMJ21T, isolated from salt-marsh plants, were determined to be related to species of the genus Vibriofrom from 16S rRNA sequence comparisons. Their closest phylogenetic relatives are members of the Gazogenes clade, Vibrio mangrovi and Vibrio rhizosphaerae , which show the greatest similarity to the SMJ21TrRNA sequence (97.3 and 97.1 %, respectively), while EAod9T had less than 97.0 % similarity to any other species of the genus Vibrio. Both strains share the basic characteristics of the genus Vibrio, as they are Gram-stain negative, motile, slightly halophilic, facultatively anaerobic bacteria. In addition, they are oxidase-negative and unable to grow on TCBS Agar; they grow between 15 to 26 °C, pH 6 to 8 and in up to 10 % (w/v) total salinity. They produce indol, are positive in the Voges-Proskauer test and are negative for arginine dihydrolase, lysine and ornithine decarboxylases. Strain SMJ21T is aerogenic and red-pigmented, due to prodigiosin production, while strain EAod9T ferments glucose without gas and is not pigmented. The major cellular fatty acids of both novel strains were C16 : 1ω7c/C16 : 1ω6c and C16 : 0. WGSobtained for both strains, along with the other five members of the clade, allowed the determination of ANI indexes and in silico estimations of DDH values, which confirmed that the two strains represent two novel species of the genus Vibrio: Vibriopalustris sp. nov. (with EAod9T=CECT 9027T=LMG 29724T as the proposed type strain) and Vibrio spartinae sp. nov. (with SMJ21T=CECT 9026T=LMG 29723T as the proposed type strain).
[Mh] Termos MeSH primário: Amaranthaceae/microbiologia
Filogenia
Poaceae/microbiologia
Vibrio/classificação
Zonas Úmidas
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Pigmentação
RNA Ribossômico 16S/genética
Plantas Tolerantes a Sal/microbiologia
Análise de Sequência de DNA
Espanha
Vibrio/genética
Vibrio/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002155


  7 / 7213 MEDLINE  
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[PMID]:28829580
[Au] Autor:Hjörleifsson JG; Ásgeirsson B
[Ad] Endereço:Department of Biochemistry, Science Institute, University of Iceland , Dunhagi 3, 107 Reykjavik, Iceland.
[Ti] Título:pH-Dependent Binding of Chloride to a Marine Alkaline Phosphatase Affects the Catalysis, Active Site Stability, and Dimer Equilibrium.
[So] Source:Biochemistry;56(38):5075-5089, 2017 Sep 26.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of ionic strength on enzyme activity and stability varies considerably between enzymes. Ionic strength is known to affect the catalytic activity of some alkaline phosphatases (APs), such as Escherichia coli AP, but how ions affect APs is debated. Here, we studied the effect of various ions on a cold-adapted AP from Vibrio splendidus (VAP). Previously, we have found that the active form of VAP is extremely unstable at low ionic strengths. Here we show that NaCl increased the activity and stability of VAP and that the effect was pH-dependent in the range of pH 7-10. The activity profile as a function of pH formed two maxima, indicating a possible conformational change. Bringing the pH from the neutral to the alkaline range was accompanied by a large increase in both the K for inorganic phosphate (product inhibition) and the K for p-nitrophenyl phosphate. The activity transitions observed as the pH was varied correlated with structural changes as monitored by tryptophan fluorescence. Thermal and urea-induced inactivation was shown to be accompanied by neither dissociation of the active site metal ions nor dimer dissociation. This would suggest that the inactivation involved subtle changes in active site conformation. Furthermore, the VAP dimer equilibrium was studied for the first time and shown to highly favor dimerization, which was dependent on pH and NaCl concentration. Taken together, the data support a model in which anions bind to some specific acceptor in the active site of VAP, resulting in great stabilization and catalytic rate enhancement, presumably through a different mechanism.
[Mh] Termos MeSH primário: Fosfatase Alcalina/química
Fosfatase Alcalina/metabolismo
Cloretos/metabolismo
Vibrio/enzimologia
[Mh] Termos MeSH secundário: Fosfatase Alcalina/genética
Organismos Aquáticos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Catálise
Domínio Catalítico
Ativação Enzimática
Estabilidade Enzimática
Fluorescência
Concentração de Íons de Hidrogênio
Magnésio/metabolismo
Concentração Osmolar
Fosforilação
Desnaturação Proteica
Multimerização Proteica
Triptofano
Ureia/química
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Chlorides); 8DUH1N11BX (Tryptophan); 8W8T17847W (Urea); EC 3.1.3.1 (Alkaline Phosphatase); I38ZP9992A (Magnesium); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00690


  8 / 7213 MEDLINE  
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[PMID]:28792420
[Au] Autor:Ji A; Li X; Fang S; Qin Z; Bai C; Wang C; Zhang Z
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, PR China.
[Ti] Título:Primary culture of Zhikong scallop Chlamys farreri hemocytes as an in vitro model for studying host-pathogen interactions.
[So] Source:Dis Aquat Organ;125(3):217-226, 2017 08 09.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Primary cultured cells can be a useful tool in studies on physiology, virology, and toxicology. Hemocytes play an important role in animal rapid response to pathogen invasion. In this study, an appropriate medium for primary culture of hemocytes of the bivalve Chlamys farreri was developed by adding 5% fetal bovine serum and 1% C. farreri serum to Leibovitz L-15 medium. These primary cultured hemocytes were maintained for more than 40 d in vitro and were classified into 3 types: (1) granulocytes containing numerous granules in the cytoplasm, (2) hyalinocytes with no or few granules, (3) a small percentage of macrophage-like cells. Furthermore, the primary cultured hemocytes were observed to be sensitive to bacterial and viral challenges. These hemocytes could phagocytose the bacterium Vibrio anguillarum, and presented cytopathic effects on the extracellular products (ECPs) of V. anguillarum; the mRNA level of QM, which plays an important role in immune response, also significantly increased 12 h after infection. When these hemocytes were challenged with ostreid herpesvirus 1 (OsHV-1), virus particles and empty capsids in the cells infected for 48 h were observed by transmission electron microscopy, and the QM mRNA level increased significantly at 12 h and 24 h following OsHV-1 challenge. This primary culture system is available for C. farreri hemocytes which can be used in the future to study host-pathogen interactions.
[Mh] Termos MeSH primário: Hemócitos/fisiologia
Herpesviridae/fisiologia
Pectinidae/citologia
Vibrio/fisiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células
Regulação da Expressão Gênica/imunologia
Hemócitos/microbiologia
Interações Hospedeiro-Patógeno
RNA Viral
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.3354/dao03145


  9 / 7213 MEDLINE  
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[PMID]:28792417
[Au] Autor:Gulla S; Rønneseth A; Sørum H; Vågnes Ø; Balboa S; Romalde JL; Colquhoun DJ
[Ad] Endereço:Norwegian Veterinary Institute, Pb 750 Sentrum, 0106 Oslo, Norway.
[Ti] Título:Vibrio tapetis from wrasse used for ectoparasite bio-control in salmon farming: phylogenetic analysis and serotyping.
[So] Source:Dis Aquat Organ;125(3):189-197, 2017 08 09.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:So-called 'cleaner fish', including various wrasse (Labridae) species, have become increasingly popular in Norwegian salmon farming in recent years for biocontrol of the salmon louse Lepeophtheirus salmonis. Cleaner fish mortalities in salmon farms are, however, often high. Various bacterial agents are frequently associated with episodes of increased cleaner fish mortality, and Vibrio tapetis is regularly cultured from diseased wrasse. In the present study, we investigated the genetic relationships among 54 V. tapetis isolates (34 from wrasse species) by multilocus sequence analysis (MLSA; rpoD, ftsZ, pyrH, rpoA and atpA). In the resulting phylogenetic tree, all wrasse isolates belonged to sub-clusters within V. tapetis subsp. tapetis. Slide agglutination testing further confirmed the complete dominance amongst these isolates of 4 O-antigen serotypes, designated here as V. tapetis subsp. tapetis serotypes O1, O3, O4 and O5, respectively. A pilot challenge trial using serotypes O3, O4 and O5 did not indicate high pathogenicity towards ballan wrasse Labrus bergylta, thus questioning the role of V. tapetis as a primary pathogen of this fish species.
[Mh] Termos MeSH primário: Agentes de Controle Biológico
Copépodes/microbiologia
Ectoparasitoses/veterinária
Doenças dos Peixes/parasitologia
Vibrio/genética
Vibrio/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Ectoparasitoses/prevenção & controle
Doenças dos Peixes/prevenção & controle
Peixes
Filogenia
Projetos Piloto
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biological Control Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.3354/dao03140


  10 / 7213 MEDLINE  
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[PMID]:28662104
[Au] Autor:Tanaka M; Endo S; Kotake F; Al-Saari N; Amin AKMR; Feng G; Mino S; Doi H; Ogura Y; Hayashi T; Suda W; Hattori M; Yumoto I; Sawabe T; Sawabe T; Araki T
[Ad] Endereço:Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University, Hakodate, Japan.
[Ti] Título:Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed.
[So] Source:PLoS One;12(6):e0180053, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1% based on the genome sequence. Major traits of the strain were non-motile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).
[Mh] Termos MeSH primário: Alga Marinha/microbiologia
Vibrio/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Genes Bacterianos
Hibridização de Ácido Nucleico
Filogenia
RNA Ribossômico 16S/genética
Vibrio/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180053



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