Base de dados : MEDLINE
Pesquisa : B03.440.520 [Categoria DeCS]
Referências encontradas : 241 [refinar]
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[PMID]:28323899
[Au] Autor:Huan YW; Bengtsson RJ; MacIntyre N; Guthrie J; Finlayson H; Smith SH; Archibald AL; Ait-Ali T
[Ad] Endereço:The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian, United Kingdom.
[Ti] Título:Lawsonia intracellularis exploits ß-catenin/Wnt and Notch signalling pathways during infection of intestinal crypt to alter cell homeostasis and promote cell proliferation.
[So] Source:PLoS One;12(3):e0173782, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lawsonia intracellularis is an obligate intracellular bacterial pathogen that causes proliferative enteropathy (PE) in pigs. L. intracellularis infection causes extensive intestinal crypt cell proliferation and inhibits secretory and absorptive cell differentiation. However, the affected host upstream cellular pathways leading to PE are still unknown. ß-catenin/Wnt signalling is essential in maintaining intestinal stem cell (ISC) proliferation and self-renewal capacity, while Notch signalling governs differentiation of secretory and absorptive lineage specification. Therefore, in this report we used immunofluorescence (IF) and quantitative reverse transcriptase PCR (RTqPCR) to examine ß-catenin/Wnt and Notch-1 signalling levels in uninfected and L. intracellularis infected pig ileums at 3, 7, 14, 21 and 28 days post challenge (dpc). We found that while the significant increase in Ki67+ nuclei in crypts at the peak of L. intracellularis infection suggested enhanced cell proliferation, the expression of c-MYC and ASCL2, promoters of cell growth and ISC proliferation respectively, was down-regulated. Peak infection also coincided with enhanced cytosolic and membrane-associated ß-catenin staining and induction of AXIN2 and SOX9 transcripts, both encoding negative regulators of ß-catenin/Wnt signalling and suggesting a potential alteration to ß-catenin/Wnt signalling levels, with differential regulation of the expression of its target genes. We found that induction of HES1 and OLFM4 and the down-regulation of ATOH1 transcript levels was consistent with the increased Notch-1 signalling in crypts at the peak of infection. Interestingly, the significant down-regulation of ATOH1 transcript levels coincided with the depletion of MUC2 expression at 14 dpc, consistent with the role of ATOH1 in promoting goblet cell maturation. The lack of significant change to LGR5 transcript levels at the peak of infection suggested that the crypt hyperplasia was not due to the expansion of ISC population. Overall, simultaneous induction of Notch-1 signalling and the attenuation of ß-catenin/Wnt pathway appear to be associated with the inhibition of goblet cell maturation and enhanced crypt cell proliferation at the peak of L. intracellularis infection. Moreover, the apparent differential regulation of apoptosis between crypt and lumen cells together with the strong induction of Notch-1 signalling and the enhanced SOX9 expression along crypts 14 dpc suggest an expansion of actively dividing transit amplifying and/or absorptive progenitor cells and provide a potential basis for understanding the development and maintenance of PE.
[Mh] Termos MeSH primário: Infecções por Desulfovibrionaceae/metabolismo
Lawsonia (Bactéria)
Receptores Notch/metabolismo
Via de Sinalização Wnt/fisiologia
beta Catenina/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Caspase 3/metabolismo
Proliferação Celular/fisiologia
Infecções por Desulfovibrionaceae/patologia
Progressão da Doença
Feminino
Homeostase/fisiologia
Íleo/metabolismo
Íleo/microbiologia
Íleo/patologia
Masculino
Mucina-2/metabolismo
Distribuição Aleatória
Fatores de Transcrição SOX9/metabolismo
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mucin-2); 0 (Receptors, Notch); 0 (SOX9 Transcription Factor); 0 (beta Catenin); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173782


  2 / 241 MEDLINE  
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[PMID]:28115029
[Au] Autor:Le Bourgot C; Le Normand L; Formal M; Respondek F; Blat S; Apper E; Ferret-Bernard S; Le Huërou-Luron I
[Ad] Endereço:1French National Institute for Agricultural Research (INRA),UR1341 Food and Digestive, Nervous and Behavioural Adaptations (ADNC),Saint-Gilles,F-35590,France.
[Ti] Título:Maternal short-chain fructo-oligosaccharide supplementation increases intestinal cytokine secretion, goblet cell number, butyrate concentration and Lawsonia intracellularis humoral vaccine response in weaned pigs.
[So] Source:Br J Nutr;117(1):83-92, 2017 Jan.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Prebiotic supplementation modulates immune system development and function. However, less is known about the effects of maternal prebiotic consumption on offspring intestinal defences and immune system responsiveness. We investigated the effects of maternal short-chain fructo-oligosaccharide (scFOS) supplementation on mucin-secreting cells, ileal secretory IgA and cytokine secretion of weaned offspring and their humoral response to an oral vaccine against obligate intracellular Lawsonia intracellularis. Sows were fed a control diet (CTRL) or scFOS-supplemented diet during the last third of gestation and throughout lactation. At weaning, each litter was divided into two groups receiving a post-weaning CTRL or scFOS diet for a month. Pigs from the four groups were either non-vaccinated (n 16) or vaccinated (n 117) at day 33. Biomarkers related to intestinal defences and immune parameters were analysed 3 weeks later. SCFA production was assessed over time in suckling and weaned pigs. Maternal scFOS supplementation improved ileal cytokine secretions (interferon (IFN)-γ, P<0·05; IL-4, P=0·07) and tended to increase caecal goblet cell number (P=0·06). It increased IgA vaccine response in the serum (P<0·01) and ileal mucosa (P=0·08). Higher bacterial fermentative activity was observed during lactation (total faecal SCFA, P<0·001) and after weaning (colonic butyrate, P=0·10) in pigs from scFOS-supplemented mothers. No synergistic effect between maternal and post-weaning scFOS supplementation was observed. Therefore, maternal scFOS supplementation has long-lasting consequences by strengthening gut defences and immune response to a vaccine against an intestinal obligate intracellular pathogen. Prebiotic consumption by gestating and lactating mothers is decisive in modulating offspring intestinal immunity.
[Mh] Termos MeSH primário: Vacinas Bacterianas/imunologia
Butiratos/sangue
Citocinas/metabolismo
Células Caliciformes/fisiologia
Lawsonia (Bactéria)
Oligossacarídeos/administração & dosagem
[Mh] Termos MeSH secundário: Ração Animal/análise
Fenômenos Fisiológicos da Nutrição Animal
Animais
Citocinas/genética
Infecções por Desulfovibrionaceae/microbiologia
Infecções por Desulfovibrionaceae/veterinária
Dieta/veterinária
Suplementos Nutricionais
Feminino
Fenômenos Fisiológicos da Nutrição Materna
Oligossacarídeos/química
Prebióticos
Suínos
Doenças dos Suínos/prevenção & controle
[Pt] Tipo de publicação:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Vaccines); 0 (Butyrates); 0 (Cytokines); 0 (Oligosaccharides); 0 (Prebiotics); 0 (fructooligosaccharide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114516004268


  3 / 241 MEDLINE  
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[PMID]:28034455
[Au] Autor:Ohta T; Kimura K; Katsuda K; Kobayashi H; Mikami O; Haritani M; Onodera T
[Ad] Endereço:Hyogo Prefectural Meat Inspection Centre, Kakogawa, Hyogo, Japan.
[Ti] Título:Proliferative Enteropathy caused by Lawsonia intracellularis in Chickens.
[So] Source:J Comp Pathol;156(2-3):158-161, 2017 Feb - Apr.
[Is] ISSN:1532-3129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Proliferative enteropathy (PE) is an infectious disease caused by Lawsonia intracellularis (Li), an obligate intracellular bacterium. PE is endemic in swine herds and has been reported in a variety of mammals including horses, hamsters, rabbits, rats, guinea pigs, ferrets, foxes, dogs, sheep, deer and non-human primates. Avian cases are reported only in ratite birds, including emus and ostriches. Some studies show an absence of Lawsonia spp. infection in chickens. In this study, we performed morphological and bacteriological examinations on the intestines of two broiler chickens that had been condemned at a poultry slaughter plant in Japan due to intestinal haemorrhage, which was a result of focal coccidial enteritis. Histopathology revealed proliferation of the villous epithelium in the small and/or large intestines, especially the caeca, regardless of coccidial lesions. Warthin-Starry silver staining and immunohistochemistry using anti-Li monoclonal antibody revealed numerous bacteria and/or antigens in the villous epithelium. Scanning electron microscopy and transmission electron microscopy revealed the presence of curved rods, morphologically compatible with Li, in the apical cytoplasm of the epithelium. Polymerase chain reaction products specific for Li were amplified from DNA samples extracted from formalin-fixed and paraffin wax-embedded tissue. These results suggest that Li can cause PE, characterized by proliferation of the villous epithelium, in chickens.
[Mh] Termos MeSH primário: Galinhas/microbiologia
Infecções por Desulfovibrionaceae/veterinária
Enterite/veterinária
Doenças das Aves Domésticas/patologia
[Mh] Termos MeSH secundário: Animais
Imuno-Histoquímica
Lawsonia (Bactéria)
Microscopia Eletrônica
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE


  4 / 241 MEDLINE  
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[PMID]:27916787
[Au] Autor:Li H; Zhang M; Zheng E
[Ad] Endereço:Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, College of Life Science, Longyan University, Fujian, 364012, China.
[Ti] Título:Comprehensive miRNA expression profiles in the ilea of Lawsonia intracellularis-infected pigs.
[So] Source:J Vet Med Sci;79(2):282-289, 2017 Feb 14.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To analyze the miRNA expression profiles in Lawsonia intracellularis-infected porcine intestines, infected pigs were first identified using PCR (Polymerase Chain Reaction). Then, RNA from infected intestines and control tissues were isolated and subjected to microarray analysis and RT-PCR. Results showed that a total of 83 miRNAs were differentially expressed between the infected samples and controls, out of which 53 were upregulated and 30 were downregulated. Validation using RT-PCR showed a high degree of confidence for the obtained data. Using the current miRBase release 21.0, nine groups of miRNAs were located in the same cluster, and three groups of miRNAs were found to belong to the same family. Interestingly, except for ssc-miR-10a-5p, all clustered miRNAs and the family members exhibited the same expression patterns. Pathway analysis of the putative gene targets of the differentially expressed miRNAs showed that they were involved in the immune response, amino acid metabolism and cell communication/growth/motility. Thus, the results indicate that altered miRNA expression profiles can affect immunity, metabolism and cellular processes.
[Mh] Termos MeSH primário: Infecções por Desulfovibrionaceae/veterinária
Íleo/metabolismo
Lawsonia (Bactéria)
MicroRNAs/metabolismo
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Infecções por Desulfovibrionaceae/metabolismo
Infecções por Desulfovibrionaceae/microbiologia
Íleo/microbiologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Suínos/metabolismo
Suínos/microbiologia
Doenças dos Suínos/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0423


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[PMID]:27487502
[Au] Autor:Järveots T; Saar T; Põdersoo D; Rüütel-Boudinot S; Sütt S; Tummeleht L; Suuroja T; Lindjärv R
[Ti] Título:Lawsonia intracellularis and Porcine Circovirus type-2 infection in Estonia.
[So] Source:Pol J Vet Sci;19(2):291-301, 2016.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The present study describes the reasons of post-weaning distress in Estonian pig herds. Here we examined the natural cases of Lawsonia intracellularis and porcine circovirus 2 (PCV2) infection and co-infections. The presence of L. intracellularis in swine herds were tested by PCR and by histopathological methods, whereas PCV2 was detected by real-time-PCR and immunohistochemical stainings. Seven of the 11 investigated herds with signs of post-weaning wasting were infected with L. intracellularis and all 11 herds with PCV2. From the analysed samples 22.2% were infected with L. intracellularis and 25% with PCV2. The results of microbiological studies suggested that the piglets suffered from enteritis and pneumonia. Escherichia coli and Pasteurella multocida often aggravated the process of illness. The frequency of L. intracellularis was high in pigs 7-12 weeks old (18.5-42.7%) and PCV2 infection was too high in pigs 7-12 weeks old (24.8-32.7%). E. coli was often a co-factor with L. intracellularis and PCV2. The primary reasons of post weaning wasting were PCV2 and E. coli, later aggravated by L. intracellularis and other pathogens. Our results indicated that different pathogens have an important role in developing post-weaning wasting. Proliferative intestinal inflammation caused by L. intracellularis is mainly characterised by its localization and morphological findings. The main gross lesions were the enlargement of mesenteric lymph nodes and thickening of the wall of ileum. In post-weaning multi-systemic wasting syndrome there are characteristic histological lesions in lymphoid tissues. They consist of a variable degree of lymphocyte depletion, together with histiocytic and/or multinucleate giant cell infiltration. This basic lymphoid lesions is observable in almost all tissues of a single severely affected animal, including lymph nodes, Peyer's patches and spleen. Sporadically, multifocal coagulative necrosis may be observed.
[Mh] Termos MeSH primário: Circovirus/isolamento & purificação
Infecções por Desulfovibrionaceae/veterinária
Lawsonia (Bactéria)/isolamento & purificação
Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Circovirus/classificação
Infecções por Desulfovibrionaceae/epidemiologia
Infecções por Desulfovibrionaceae/microbiologia
Estônia/epidemiologia
Íleo/patologia
Síndrome Definhante Multissistêmico de Suínos Desmamados/epidemiologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170107
[Lr] Data última revisão:
170107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160804
[St] Status:MEDLINE


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[PMID]:27154728
[Au] Autor:Obradovic M; Pasternak JA; Ng SH; Wilson HL
[Ad] Endereço:Vaccine & Infectious Disease Organization, International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada.
[Ti] Título:Use of flow cytometry and PCR analysis to detect 5-carboxyfluoroscein-stained obligate intracellular bacteria Lawsonia intracellularis invasion of McCoy cells.
[So] Source:J Microbiol Methods;126:60-6, 2016 07.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we describe a method to quantify invasion of obligate intracellular bacteria, Lawsonia intracellularis, inside McCoy cells. In immunological research, the cell-permeable fluorescent dye 5'-carboxyfluoroscein succidyl ester (CFSE) is commonly used to quantify eukaryotic cellular proliferation. Instead of using it in this traditional way, we stained L. intracellularis with CFSE dye prior to infection of McCoy cells. Flow cytometry was performed to quantify the percentage of eukaryotic cells which had taken up or were associated with fluorescent bacteria. As obligate intracellular bacteria, they cannot replicate outside of eukaryotic cells and thus qPCR analysis was used to quantify bacterial growth. Indirectly, PCR analysis confirmed invasion rather than adherence to the McCoy cell surface. Fluorescent activated cell sorting (FACS) was used to sort the CFSE(+) (i.e. infected) McCoy cells from the CFSE(-) (i.e. non-infected) McCoy cells and confocal microscopy was used to confirm bacterial invasion and cytosolic localization of CFSE-L. intracellularis. To show that this approach could be used in conjunction with functional assays, we investigated the effect that serum antibodies had on CFSE-bacterial invasion and growth. Instead of blocking invasion, rabbit hyperimmune serum augmented invasion of the bacteria inside McCoy cells and qPCR analysis confirmed bacterial growth over the course of 5days. We conclude that CFSE-labeling of bacteria and qPCR can be used to track and quantify bacterial invasion and may be a valuable tool for studying the invasive properties of bacteria, especially if commercial antibodies are not available. This approach may be adapted for use in other obligate intracellular bacteria and intracellular pathogens.
[Mh] Termos MeSH primário: Citoplasma/microbiologia
Citometria de Fluxo/métodos
Lawsonia (Bactéria)/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
Anticorpos/farmacologia
Aderência Bacteriana
Fluoresceínas
Lawsonia (Bactéria)/efeitos dos fármacos
Lawsonia (Bactéria)/crescimento & desenvolvimento
Lawsonia (Bactéria)/metabolismo
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies); 0 (Fluoresceins); 76823-03-5 (4-carboxyfluorescein)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE


  7 / 241 MEDLINE  
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[PMID]:27031415
[Au] Autor:Komine M; Cunha TO; Mullaney TP; Smedley RC; Langohr IM
[Ti] Título:Pathology in Practice. Proliferative and necrotizing enterocolitis in a pig resulting from coinfection with L intracellularis and S enterica.
[So] Source:J Am Vet Med Assoc;248(8):897-9, 2016 Apr 15.
[Is] ISSN:1943-569X
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções por Desulfovibrionaceae/veterinária
Enterocolite Necrosante/veterinária
Lawsonia (Bactéria)/isolamento & purificação
Salmonelose Animal/patologia
Salmonella enterica/isolamento & purificação
Doenças dos Suínos/patologia
[Mh] Termos MeSH secundário: Animais
Infecções por Circoviridae/complicações
Infecções por Circoviridae/veterinária
Circovirus/isolamento & purificação
Coinfecção
Infecções por Desulfovibrionaceae/complicações
Infecções por Desulfovibrionaceae/patologia
Enterocolite Necrosante/microbiologia
Enterocolite Necrosante/patologia
Salmonelose Animal/complicações
Suínos
Doenças dos Suínos/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160401
[St] Status:MEDLINE
[do] DOI:10.2460/javma.248.8.897


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[PMID]:26774445
[Au] Autor:Larsen I; Nielsen SS; Olsen JE; Nielsen JP
[Ad] Endereço:University of Copenhagen, Faculty of Health and Medical Sciences, Department of Large Animal Sciences, Grønnegårdsvej 2, 1870 Frederiksberg C, Denmark.
[Ti] Título:The efficacy of oxytetracycline treatment at batch, pen and individual level on Lawsonia intracellularis infection in nursery pigs in a randomised clinical trial.
[So] Source:Prev Vet Med;124:25-33, 2016 Feb 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Antimicrobial consumption in animal husbandry is of great scientific and political concern due to the risk of selection of resistant bacteria. Whilst a reduction in the use of antimicrobials is therefore preferable, the efficacy of treatment must be maintained in order to ensure animal welfare and profitability of pig production. The objective of this study was to evaluate the efficacy of three treatment strategies under field conditions against Lawsonia intracellularis (LI)-related diarrhoea. A randomised clinical trial was carried out in four Danish pig herds, including a total of 520 pigs from 36 nursery batches. A high prevalence of LI was demonstrated in all herds prior to the initiation of the study. Treatment efficacy was assessed by faecal shedding of LI, the occurrence of diarrhoea and average daily weight gain (ADG) after treatment. All strategies were implemented at batch level at presence of LI-related diarrhoea and included daily treatment with 10mg oxytetracycline (OTC) per kilogram of bodyweight for 5 days, though the OTC was administered differently: either by oral treatment of all pigs in a batch, by oral treatment of pigs in diarrhoeic pens only, or by intramuscular treatment of individual diarrhoeic pigs only. The treatment strategies were randomly allocated to batches and were initiated at the presence of diarrhoea. From the included batches, 100% of the trial pigs were medicated in the batch treatment strategy, 87% in the pen treatment strategy and 55% in the individual treatment strategy. All strategies reduced the occurrence of diarrhoea and faecal shedding of LI after treatment. However, batch treatment was found to be most efficient in reducing both high-level LI shedding and diarrhoea when compared to the treatment of diarrhoeic pens or individual diarrhoeic pigs. There was no significant difference identified in ADG between the treatment strategies. In conclusion, batch treatment of all pigs in a section resulted in the highest efficacy for reducing diarrhoea and faecal shedding of LI.
[Mh] Termos MeSH primário: Infecções por Desulfovibrionaceae/veterinária
Diarreia/veterinária
Lawsonia (Bactéria)/efeitos dos fármacos
Oxitetraciclina/uso terapêutico
Doenças dos Suínos/tratamento farmacológico
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anti-Infecciosos/farmacologia
Anti-Infecciosos/uso terapêutico
Derrame de Bactérias
Infecções por Desulfovibrionaceae/tratamento farmacológico
Diarreia/tratamento farmacológico
Fezes/microbiologia
Feminino
Injeções Intramusculares/veterinária
Masculino
Oxitetraciclina/farmacologia
Suínos
Resultado do Tratamento
Ganho de Peso/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); X20I9EN955 (Oxytetracycline)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160118
[St] Status:MEDLINE


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[PMID]:26718056
[Au] Autor:Larsen I; Hjulsager CK; Holm A; Olsen JE; Nielsen SS; Nielsen JP
[Ad] Endereço:University of Copenhagen, Faculty of Health and Medical Sciences, Department of Large Animal Sciences, Grønnegårdsvej 2, 1870 Frederiksberg C, Denmark. Electronic address: soenderhedelund@gmail.com.
[Ti] Título:A randomised clinical trial on the efficacy of oxytetracycline dose through water medication of nursery pigs on diarrhoea, faecal shedding of Lawsonia intracellularis and average daily weight gain.
[So] Source:Prev Vet Med;123:52-59, 2016 Jan 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Oral treatment with antimicrobials is widely used in pig production for the control of gastrointestinal infections. Lawsonia intracellularis (LI) causes enteritis in pigs older than six weeks of age and is commonly treated with antimicrobials. The objective of this study was to evaluate the efficacy of three oral dosage regimens (5, 10 and 20mg/kg body weight) of oxytetracycline (OTC) in drinking water over a five-day period on diarrhoea, faecal shedding of LI and average daily weight gain (ADG). A randomised clinical trial was carried out in four Danish pig herds. In total, 539 animals from 37 batches of nursery pigs were included in the study. The dosage regimens were randomly allocated to each batch and initiated at presence of assumed LI-related diarrhoea. In general, all OTC doses used for the treatment of LI infection resulted in reduced diarrhoea and LI shedding after treatment. Treatment with a low dose of 5mg/kg OTC per kg body weight, however, tended to cause more watery faeces and resulted in higher odds of pigs shedding LI above detection level when compared to medium and high doses (with odds ratios of 5.5 and 8.4, respectively). No association was found between the dose of OTC and the ADG. In conclusion, a dose of 5mg OTC per kg body weight was adequate for reducing the high-level LI shedding associated with enteropathy, but a dose of 10mg OTC per kg body weight was necessary to obtain a maximum reduction in LI shedding.
[Mh] Termos MeSH primário: Infecções por Desulfovibrionaceae/veterinária
Diarreia/veterinária
Lawsonia (Bactéria)/efeitos dos fármacos
Oxitetraciclina/farmacologia
Oxitetraciclina/uso terapêutico
Doenças dos Suínos/tratamento farmacológico
[Mh] Termos MeSH secundário: Administração Oral
Animais
Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Derrame de Bactérias/efeitos dos fármacos
Infecções por Desulfovibrionaceae/tratamento farmacológico
Infecções por Desulfovibrionaceae/microbiologia
Diarreia/tratamento farmacológico
Diarreia/microbiologia
Relação Dose-Resposta a Droga
Fezes/microbiologia
Feminino
Lawsonia (Bactéria)/fisiologia
Masculino
Suínos
Ganho de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); X20I9EN955 (Oxytetracycline)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160101
[St] Status:MEDLINE


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[PMID]:26812826
[Au] Autor:Dors A; Pomorska-Mól M; Czyzewska E; Wasyl D; Pejsak Z
[Ti] Título:Prevalence and risk factors for Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in finishing pigs in Polish farrow-to-finish swine herds.
[So] Source:Pol J Vet Sci;18(4):825-31, 2015.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to estimate the herd-level, within-herd prevalence, the frequency of mixed infections and risk factors for L. intracellularis, B. hyodysenteriae and Salmonella spp. in selected farrow-to-finish Polish pig herds. A total of 254 pooled fecal samples were collected from 9 to 24 week-old pigs in 70 herds. Real time PCR for detection of L. intracellularis and B. hyodysenteriae was performed. For Salmonella spp. bacteriological examination was performed. The herd-level prevalences of L. intracellularis, B. hyodysenteriae and Salmonella spp. among examined herds were 65.7%, 1.4% and 8.6%, respectively. The within-herd prevalences (in positive herds) for L. intracellularis, B. hyodysenteriae and Salmonella spp. were 51.5%, 75.0% and 30.4%, respectively. All herds with diarrhea observed during sampling were infected with L. intracellularis and 60% of herds with no diarrhea at the moment of sampling were infected with L. intracellularis (p=0.035). In herds with more than 200 sows the prevalence of Salmonella spp. was significantly higher compared to herds with less than 200 sows (p=0.027). In herds where all-in/all-out (AIAO) was respected, prevalence of L. intracellularis was significantly lower than in herds where this rule was not kept (p=0.024). Obtained results confirm that L. intracellularis is the major cause of bacterial diarrhea in finishing pigs. The present study identified AIAO and herd size as a risk factor, at the herd level, for L. intracellularis and Salmonella spp., respectively.
[Mh] Termos MeSH primário: Brachyspira hyodysenteriae
Infecções por Bactérias Gram-Negativas/veterinária
Lawsonia (Bactéria)
Salmonella/isolamento & purificação
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Criação de Animais Domésticos
Animais
Infecções por Bactérias Gram-Negativas/epidemiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Polônia/epidemiologia
Prevalência
Fatores de Risco
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160127
[Lr] Data última revisão:
160127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE



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