Base de dados : MEDLINE
Pesquisa : B03.440.540 [Categoria DeCS]
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  1 / 43 MEDLINE  
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[PMID]:29223156
[Au] Autor:But SY; Egorova SV; Khmelenina VN; Trotsenko YA
[Ad] Endereço:Skryabin Institute of Biochemistry and Physiology of Microorganisms, Laboratory of Methylotrophy, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. flash20063@rambler.ru.
[Ti] Título:Biochemical Properties and Phylogeny of Hydroxypyruvate Reductases from Methanotrophic Bacteria with Different C -Assimilation Pathways.
[So] Source:Biochemistry (Mosc);82(11):1295-1303, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the aerobic methanotrophic bacteria Methylomicrobium alcaliphilum 20Z, Methylococcus capsulatus Bath, and Methylosinus trichosporium OB3b, the biochemical properties of hydroxypyruvate reductase (Hpr), an indicator enzyme of the serine pathway for assimilation of reduced C -compounds, were comparatively analyzed. The recombinant Hpr obtained by cloning and heterologous expression of the hpr gene in Escherichia coli catalyzed NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but did not catalyze the reverse reactions of D-glycerate or glycolate oxidation. The absence of the glycerate dehydrogenase activity in the methanotrophic Hpr confirmed a key role of the enzyme in utilization of C -compounds via the serine cycle. The enzyme from Ms. trichosporium OB3b realizing the serine cycle as a sole assimilation pathway had much higher special activity and affinity in comparison to Hpr from Mm. alcaliphilum 20Z and Mc. capsulatus Bath assimilating carbon predominantly via the ribulose monophosphate (RuMP) cycle. The hpr gene was found as part of gene clusters coding the serine cycle enzymes in all sequenced methanotrophic genomes except the representatives of the Verrucomicrobia phylum. Phylogenetic analyses revealed two types of Hpr: (i) Hpr of methanotrophs belonging to the Gammaproteobacteria class, which use the serine cycle along with the RuMP cycle, as well as of non-methylotrophic bacteria belonging to the Alphaproteobacteria class; (ii) Hpr of methylotrophs from Alpha- and Betaproteobacteria classes that use only the serine cycle and of non-methylotrophic representatives of Betaproteobacteria. The putative role and origin of hydroxypyruvate reductase in methanotrophs are discussed.
[Mh] Termos MeSH primário: Hidroxipiruvato Redutase/classificação
Methylococcaceae/enzimologia
Methylosinus/enzimologia
Filogenia
[Mh] Termos MeSH secundário: Alphaproteobacteria
Gammaproteobacteria
Bactérias Aeróbias Gram-Negativas/classificação
Bactérias Aeróbias Gram-Negativas/enzimologia
Hidroxipiruvato Redutase/metabolismo
Methylobacillus
Methylobacteriaceae
Methylophilaceae
Serina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
452VLY9402 (Serine); EC 1.1.1.81 (Hydroxypyruvate Reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110074


  2 / 43 MEDLINE  
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[PMID]:27939869
[Au] Autor:He R; Chen M; Ma RC; Su Y; Zhang X
[Ad] Endereço:Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China. Electronic address: heruo@zju.edu.cn.
[Ti] Título:Ammonium conversion and its feedback effect on methane oxidation of Methylosinus sporium.
[So] Source:J Biosci Bioeng;123(4):466-473, 2017 Apr.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Ammonium (NH ) is not only nitrogen source that can support methanotrophic growth, but also it can inhibit methane (CH ) oxidation by competing with CH for the active site of methane monooxygenase. NH conversion and its feedback effect on the growth and activity of methanotrophs were evaluated with Methylosinus sporium used as a model methanotroph. Nitrogen sources could affect the CH -derived carbon distribution, which varied with incubation time and nitrogen concentrations. More CH -derived carbon was incorporated into biomass in the media with NH -N, compared to nitrate-nitrogen (NO -N), as sole nitrogen source at the nitrogen concentrations of 10-18 mmol L . Although ammonia (NH ) oxidation activity of methanotrophs was considerably lower, only accounting for 0.01-0.06% of CH oxidation activity in the experimental cultures, NH conversion could lead to the pH decrease and toxic intermediates accumulation in the their habits. Compared with NH , nitrite (NO ) accumulation in the NH conversion of methanotroph had stronger inhibition on its activity, especially the joint inhibition of NO accumulation and the pH decrease during the NH -N conversion. These results suggested that more attention should be paid to the feedback effects of NH conversion by methanotrophs to understand effects of NH on CH oxidation in the environments.
[Mh] Termos MeSH primário: Compostos de Amônio/metabolismo
Retroalimentação Fisiológica
Metano/metabolismo
Methylosinus/metabolismo
[Mh] Termos MeSH secundário: Amônia/metabolismo
Ligação Competitiva
Biomassa
Domínio Catalítico
Concentração de Íons de Hidrogênio
Methylosinus/enzimologia
Methylosinus/crescimento & desenvolvimento
Nitratos/metabolismo
Nitritos/metabolismo
Nitrogênio/metabolismo
Oxirredução
Oxigenases/química
Oxigenases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Nitrates); 0 (Nitrites); 7664-41-7 (Ammonia); EC 1.13.- (Oxygenases); EC 1.14.13.25 (methane monooxygenase); N762921K75 (Nitrogen); OP0UW79H66 (Methane)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


  3 / 43 MEDLINE  
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[PMID]:27666984
[Au] Autor:Patel SK; Jeong JH; Mehariya S; Otari SV; Madan B; Haw JR; Lee JK; Zhang L; Kim IW
[Ad] Endereço:Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
[Ti] Título:Production of Methanol from Methane by Encapsulated .
[So] Source:J Microbiol Biotechnol;26(12):2098-2105, 2016 Dec 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Massive reserves of methane (CH4) remain unexplored as a feedstock for the production of liquid fuels and chemicals, mainly because of the lack of economically suitable and sustainable strategies for selective oxidation of CH4 to methanol. The present study demonstrates the bioconversion of CH4 to methanol mediated by Type I methanotrophs, such as and . Furthermore, immobilization of a Type II methanotroph, , was carried out using different encapsulation methods, employing sodium-alginate (Na-alginate) and silica gel. The encapsulated cells demonstrated higher stability for methanol production. The optimal pH, temperature, and agitation rate were determined to be pH 7.0, 30°C, and 175 rpm, respectively, using inoculum (1.5 mg of dry cell mass/ml) and 20% of CH4 as a feed. Under these conditions, maximum methanol production (3.43 and 3.73 mM) by the encapsulated cells was recorded. Even after six cycles of reuse, the Na-alginate and silica gel encapsulated cells retained 61.8% and 51.6% of their initial efficiency for methanol production, respectively, in comparison with the efficiency of 11.5% observed in the case of free cells. These results suggest that encapsulation of methanotrophs is a promising approach to improve the stability of methanol production.
[Mh] Termos MeSH primário: Microbiologia Industrial/métodos
Metano/metabolismo
Metanol/metabolismo
Methylosinus/metabolismo
[Mh] Termos MeSH secundário: Alginatos/química
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Concentração de Íons de Hidrogênio
Methylosinus/química
Temperatura Ambiente
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Hexuronic Acids); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); OP0UW79H66 (Methane); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170322
[Lr] Data última revisão:
170322
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1608.08053


  4 / 43 MEDLINE  
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[PMID]:27371792
[Au] Autor:Patel SK; Mardina P; Kim D; Kim SY; Kalia VC; Kim IW; Lee JK
[Ad] Endereço:Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
[Ti] Título:Improvement in methanol production by regulating the composition of synthetic gas mixture and raw biogas.
[So] Source:Bioresour Technol;218:202-8, 2016 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Raw biogas can be an alternative feedstock to pure methane (CH4) for methanol production. In this investigation, we evaluated the methanol production potential of Methylosinus sporium from raw biogas originated from an anaerobic digester. Furthermore, the roles of different gases in methanol production were investigated using synthetic gas mixtures of CH4, carbon dioxide (CO2), and hydrogen (H2). Maximum methanol production was 5.13, 4.35, 6.28, 7.16, 0.38, and 0.36mM from raw biogas, CH4:CO2, CH4:H2, CH4:CO2:H2, CO2, and CO2:H2, respectively. Supplementation of H2 into raw biogas increased methanol production up to 3.5-fold. Additionally, covalent immobilization of M. sporium on chitosan resulted in higher methanol production from raw biogas. This study provides a suitable approach to improve methanol production using low cost raw biogas as a feed containing high concentrations of H2S (0.13%). To our knowledge, this is the first report on methanol production from raw biogas, using immobilized cells of methanotrophs.
[Mh] Termos MeSH primário: Dióxido de Carbono/química
Hidrogênio/química
Metano/química
Metanol/química
[Mh] Termos MeSH secundário: Anaerobiose
Biocombustíveis
Biotecnologia
Gases
Methylosinus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Gases); 142M471B3J (Carbon Dioxide); 7YNJ3PO35Z (Hydrogen); OP0UW79H66 (Methane); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160703
[St] Status:MEDLINE


  5 / 43 MEDLINE  
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[PMID]:26513269
[Au] Autor:Vavilin VA; Rytov SV; Shim N; Vogt C
[Ad] Endereço:a Russian Academy of Sciences , Ecological Department of Water Problems Institute , Moscow , Russian Federation.
[Ti] Título:Non-linear dynamics of stable carbon and hydrogen isotope signatures based on a biological kinetic model of aerobic enzymatic methane oxidation.
[So] Source:Isotopes Environ Health Stud;52(3):185-202, 2016 Jun.
[Is] ISSN:1477-2639
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The non-linear dynamics of stable carbon and hydrogen isotope signatures during methane oxidation by the methanotrophic bacteria Methylosinus sporium strain 5 (NCIMB 11126) and Methylocaldum gracile strain 14 L (NCIMB 11912) under copper-rich (8.9 µM Cu(2+)), copper-limited (0.3 µM Cu(2+)) or copper-regular (1.1 µM Cu(2+)) conditions has been described mathematically. The model was calibrated by experimental data of methane quantities and carbon and hydrogen isotope signatures of methane measured previously in laboratory microcosms reported by Feisthauer et al. [ 1 ] M. gracile initially oxidizes methane by a particulate methane monooxygenase and assimilates formaldehyde via the ribulose monophosphate pathway, whereas M. sporium expresses a soluble methane monooxygenase under copper-limited conditions and uses the serine pathway for carbon assimilation. The model shows that during methane solubilization dominant carbon and hydrogen isotope fractionation occurs. An increase of biomass due to growth of methanotrophs causes an increase of particulate or soluble monooxygenase that, in turn, decreases soluble methane concentration intensifying methane solubilization. The specific maximum rate of methane oxidation υm was proved to be equal to 4.0 and 1.3 mM mM(-1) h(-1) for M. sporium under copper-rich and copper-limited conditions, respectively, and 0.5 mM mM(-1) h(-1) for M. gracile. The model shows that methane oxidation cannot be described by traditional first-order kinetics. The kinetic isotope fractionation ceases when methane concentrations decrease close to the threshold value. Applicability of the non-linear model was confirmed by dynamics of carbon isotope signature for carbon dioxide that was depleted and later enriched in (13)C. Contrasting to the common Rayleigh linear graph, the dynamic curves allow identifying inappropriate isotope data due to inaccurate substrate concentration analyses. The non-linear model pretty adequately described experimental data presented in the two-dimensional plot of hydrogen versus carbon stable isotope signatures.
[Mh] Termos MeSH primário: Isótopos de Carbono
Deutério
Metano/metabolismo
Methylococcaceae/metabolismo
Methylosinus/metabolismo
Modelos Biológicos
Oxigenases/metabolismo
[Mh] Termos MeSH secundário: Aerobiose
Cobre/metabolismo
Cinética
Methylococcaceae/enzimologia
Methylosinus/enzimologia
Dinâmica não Linear
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 789U1901C5 (Copper); AR09D82C7G (Deuterium); EC 1.13.- (Oxygenases); EC 1.14.13.25 (methane monooxygenase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151030
[St] Status:MEDLINE
[do] DOI:10.1080/10256016.2016.1092965


  6 / 43 MEDLINE  
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[PMID]:25267420
[Au] Autor:Yoo YS; Han JS; Ahn CM; Kim CG
[Ad] Endereço:a Department of Environmental Engineering , Inha University , Incheon , Republic of Korea.
[Ti] Título:Comparative enzyme inhibitive methanol production by Methylosinus sporium from simulated biogas.
[So] Source:Environ Technol;36(5-8):983-91, 2015 Mar-Apr.
[Is] ISSN:0959-3330
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methane in a simulated biogas converting to methanol under aerobic condition was comparatively assessed by inhibiting the activity of methanol dehydrogenase (MDH) of Methylosinus sporium using phosphate, NaCl, NH4Cl or EDTA in their varying concentrations. The highest amount of methane was indistinguishably diverted at the typical conditions regardless of the types of inhibitors: 35°C and pH 7 under a 0.4% (v/v) of biogas, specifically for <40 mM phosphate, 50 mM NaCl, 40 mM NH4Cl or 150 µM EDTA. The highest level of methanol was obtained for the addition of 40 mM phosphate, 100 mM NaCl, 40 mM NH4Cl or 50 µM EDTA. In other words, 0.71, 0.60, 0.66 and 0.66 mmol methanol was correspondingly generated by the oxidation of 1.3, 0.67, 0.74 and 1.3 mmol methane. It gave a methanol conversion rate of 54.7%, 89.9%, 89.6% and 47.8%, respectively. Among them, the maximum rate of methanol production was observed at 6.25 µmol/mg h for 100 mM NaCl. Regardless of types or concentrations of inhibitors differently used, methanol production could be nonetheless identically maximized when the MDH activity was limitedly hampered by up to 35%.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/antagonistas & inibidores
Metanol/metabolismo
Methylosinus/enzimologia
[Mh] Termos MeSH secundário: Cloreto de Amônio/química
Biocombustíveis
Ácido Edético/química
Concentração de Íons de Hidrogênio
Oxirredução
Cloreto de Sódio/química
Temperatura Ambiente
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biofuels); 01Q9PC255D (Ammonium Chloride); 451W47IQ8X (Sodium Chloride); 9G34HU7RV0 (Edetic Acid); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.2.8 (alcohol dehydrogenase (acceptor)); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141001
[St] Status:MEDLINE
[do] DOI:10.1080/09593330.2014.971059


  7 / 43 MEDLINE  
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[PMID]:25436252
[Au] Autor:Kallistova AIu; Montonen L; Jurgens G; Munster U; Kevbrina MV; Nozhevnikova AN
[Ti] Título:[Culturable psychrotolerant methanotrophic bacteria in landfill cover soil].
[So] Source:Mikrobiologiia;83(1):109-18, 2014 Jan-Feb.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Methanotrophs closely related to psychrotolerant members of the genera Methylobacter and Methylocella were identified in cultures enriched at 10@C from landfill cover soil samples collected in the period from April to November. Mesophilic methanotrophs of the genera Methylobacter and Methylosinus were found in cultures enriched at 20 degrees C from the same cover soil samples. A thermotolerant methanotroph related to Methylocaldum gracile was identified in the culture enriched at 40 degrees C from a sample collected in May (the temperature of the cover soil was 11.5-12.5 degrees C). In addition to methanotrophs, methylobacteria of the genera Methylotenera and Methylovorus and members of the genera Verrucomicrobium, Pseudomonas, Pseudoxanthomonas, Dokdonella, Candidatus Protochlamydia, and Thiorhodospira were also identified in the enrichment cultures. A methanotroph closely related to the psychrotolerant species Methylobacter tundripaludum (98% sequence identity of 16S r-RNA genes with the type strain SV96(T)) was isolated in pure culture. The introduction of a mixture of the methanotrophic enrichments, grown at 15 degrees C, into the landfill cover soil resulted in a decrease in methane emission from the landfill surface in autumn (October, November). The inoculum used was demonstrated to contain methanotrophs closely related to Methylobacter tundripaludum SV96.
[Mh] Termos MeSH primário: Microbiologia do Solo
Instalações de Eliminação de Resíduos
[Mh] Termos MeSH secundário: Ectothiorhodospiraceae/genética
Ectothiorhodospiraceae/isolamento & purificação
Metano/metabolismo
Methylococcaceae/isolamento & purificação
Methylophilaceae/genética
Methylophilaceae/isolamento & purificação
Methylosinus/genética
Methylosinus/isolamento & purificação
Filogenia
Pseudomonas/genética
Pseudomonas/isolamento & purificação
RNA Ribossômico 16S
Estações do Ano
Temperatura Ambiente
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:141126
[Lr] Data última revisão:
141126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141202
[St] Status:MEDLINE


  8 / 43 MEDLINE  
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[PMID]:25261627
[Au] Autor:Ordaz A; López JC; Figueroa-González I; Muñoz R; Quijano G
[Ad] Endereço:Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain; Universidad Politécnica de Quintana Roo, Av. Tulum, Manzana 1 Lote 40, SM2, Cancún, Quintana Roo, CP 77500, Mexico.
[Ti] Título:Assessment of methane biodegradation kinetics in two-phase partitioning bioreactors by pulse respirometry.
[So] Source:Water Res;67:46-54, 2014 Dec 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biological methane biodegradation is a promising treatment alternative when the methane produced in waste management facilities cannot be used for energy generation. Two-phase partitioning bioreactors (TPPBs), provided with a non-aqueous phase (NAP) with high affinity for the target pollutant, are particularly suitable for the treatment of poorly water-soluble compounds such as methane. Nevertheless, little is known about the influence of the presence of the NAP on the resulting biodegradation kinetics in TPPBs. In this study, an experimental framework based on the in situ pulse respirometry technique was developed to assess the impact of NAP addition on the methane biodegradation kinetics using Methylosinus sporium as a model methane-degrading microorganism. A comprehensive mass transfer characterization was performed in order to avoid mass transfer limiting scenarios and ensure a correct kinetic parameter characterization. The presence of the NAP mediated significant changes in the apparent kinetic parameters of M. sporium during methane biodegradation, with variations of 60, 120, and 150% in the maximum oxygen uptake rate, half-saturation constant and maximum specific growth rate, respectively, compared with the intrinsic kinetic parameters retrieved from a control without NAP. These significant changes in the kinetic parameters mediated by the NAP must be considered for the design, operation and modeling of TPPBs devoted to air pollution control.
[Mh] Termos MeSH primário: Poluição do Ar/prevenção & controle
Reatores Biológicos
Metano/metabolismo
Methylosinus/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental/efeitos dos fármacos
Cinética
Consumo de Oxigênio/fisiologia
Óleos de Silicone/farmacologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Silicone Oils); OP0UW79H66 (Methane)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141203
[Lr] Data última revisão:
141203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140928
[St] Status:MEDLINE


  9 / 43 MEDLINE  
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[PMID]:24743729
[Au] Autor:Limbri H; Gunawan C; Thomas T; Smith A; Scott J; Rosche B
[Ad] Endereço:School of Chemical Engineering, The University of New South Wales, Sydney, Australia.
[Ti] Título:Coal-packed methane biofilter for mitigation of green house gas emissions from coal mine ventilation air.
[So] Source:PLoS One;9(4):e94641, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methane emitted by coal mine ventilation air (MVA) is a significant greenhouse gas. A mitigation strategy is the oxidation of methane to carbon dioxide, which is approximately twenty-one times less effective at global warming than methane on a mass-basis. The low non-combustible methane concentrations at high MVA flow rates call for a catalytic strategy of oxidation. A laboratory-scale coal-packed biofilter was designed and partially removed methane from humidified air at flow rates between 0.2 and 2.4 L min-1 at 30°C with nutrient solution added every three days. Methane oxidation was catalysed by a complex community of naturally-occurring microorganisms, with the most abundant member being identified by 16S rRNA gene sequence as belonging to the methanotrophic genus Methylocystis. Additional inoculation with a laboratory-grown culture of Methylosinus sporium, as investigated in a parallel run, only enhanced methane consumption during the initial 12 weeks. The greatest level of methane removal of 27.2±0.66 g methane m-3 empty bed h-1 was attained for the non-inoculated system, which was equivalent to removing 19.7±2.9% methane from an inlet concentration of 1% v/v at an inlet gas flow rate of 1.6 L min-1 (2.4 min empty bed residence time). These results show that low-cost coal packing holds promising potential as a suitable growth surface and contains methanotrophic microorganisms for the catalytic oxidative removal of methane.
[Mh] Termos MeSH primário: Carvão Mineral
Filtração/métodos
Efeito Estufa
Metano/isolamento & purificação
Metano/metabolismo
Methylosinus/metabolismo
Ventilação
[Mh] Termos MeSH secundário: Poluentes Atmosféricos/isolamento & purificação
Poluentes Atmosféricos/metabolismo
Biodegradação Ambiental
Dióxido de Carbono/metabolismo
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Coal); 142M471B3J (Carbon Dioxide); OP0UW79H66 (Methane)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0094641


  10 / 43 MEDLINE  
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[PMID]:24410836
[Au] Autor:Lee HJ; Kim SY; Kim PJ; Madsen EL; Jeon CO
[Ad] Endereço:Department of Life Science, Chung-Ang University, Seoul, Korea.
[Ti] Título:Methane emission and dynamics of methanotrophic and methanogenic communities in a flooded rice field ecosystem.
[So] Source:FEMS Microbiol Ecol;88(1):195-212, 2014 Apr.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methane emissions, along with methanotrophs and methanogens and soil chemical properties, were investigated in a flooded rice ecosystem. Methane emission increased after rice transplantation (from 7.2 to 552 mg day(-1) m(-2) ) and was positively and significantly correlated with transcripts of pmoA and mcrA genes, transcript/gene ratios of mcrA, temperature and total organic carbon. Methane flux was negatively correlated with sulfate concentration. Methanotrophs represented only a small proportion (0.79-1.75%) of the total bacterial 16S rRNA gene reads: Methylocystis (type II methanotroph) decreased rapidly after rice transplantation, while Methylosinus and unclassified Methylocystaceae (type II) were relatively constant throughout rice cultivation. Methylocaldum, Methylobacter, Methylomonas and Methylosarcina (type I) were sparse during the early period, but they increased after 60 days, and their maximum abundances were observed at 90-120 days. Of 33 218 archaeal reads, 68.3-86.6% were classified as methanogens. Methanosaeta, Methanocella, Methanosarcina and Methanobacterium were dominant methanogens, and their maximum abundances were observed at days 60-90. Only four reads were characteristic of anaerobic methanotrophs, suggesting that anaerobic methane metabolism is negligible in this rice paddy system. After completing a multivariate canonical correspondence analysis of our integrated data set, we found normalized mcrA/pmoA transcript ratios to be a promising parameter for predicting net methane fluxes emitted from rice paddy soils.
[Mh] Termos MeSH primário: Euryarchaeota/classificação
Metano/metabolismo
Methylococcaceae/metabolismo
Methylocystaceae/metabolismo
Methylosinus/metabolismo
Oryza
Microbiologia do Solo
[Mh] Termos MeSH secundário: DNA Arqueal/genética
DNA Bacteriano/genética
Euryarchaeota/metabolismo
Methylococcaceae/genética
Methylocystaceae/genética
Methylosinus/genética
Oxigenases/genética
Oxigenases/metabolismo
Polimorfismo de Fragmento de Restrição
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); EC 1.13.- (Oxygenases); OP0UW79H66 (Methane)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140114
[St] Status:MEDLINE
[do] DOI:10.1111/1574-6941.12282



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