Base de dados : MEDLINE
Pesquisa : B03.440.680 [Categoria DeCS]
Referências encontradas : 212 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 22 ir para página                         

  1 / 212 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28671525
[Au] Autor:Jung YT; Park S; Lee JS; Yoon JH
[Ad] Endereço:1​Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea 2​University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
[Ti] Título:Roseobacter ponti sp. nov., isolated from seawater.
[So] Source:Int J Syst Evol Microbiol;67(7):2189-2194, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, coccoid to oval-shaped and non-motile bacterial strain, designated MM-7T, was isolated from seawater of the Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain MM-7T grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2-3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MM-7T joins the branch comprising the species of the genus Roseobacter, clustering with the type strains of Roseobacter litoralis and Roseobacter denitrificans, with which it exhibited 97.9 and 96.8 % sequence similarity values, respectively. The DNA G+C content of strain MM-7T was determined to be 60.8 mol%, and its mean DNA-DNA relatedness values with Rsb. litoralis JCM 21268T was 10.3±0.4 %. Strain MM-7T contained Q-10 as the predominant ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acid. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain MM-7T is distinguishable from other species of the genus Roseobacter. On the basis of the data presented, strain MM-7T is considered to represent a novel species of the genus Roseobacter, for which the name Roseobacter ponti sp. nov. is proposed. The type strain is MM-7T (=KCTC 52469T=NBRC 112431T).
[Mh] Termos MeSH primário: Filogenia
Roseobacter/classificação
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Roseobacter/genética
Roseobacter/isolamento & purificação
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001922


  2 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28486660
[Au] Autor:Trautwein K; Feenders C; Hulsch R; Ruppersberg HS; Strijkstra A; Kant M; Vagts J; Wünsch D; Michalke B; Maczka M; Schulz S; Hillebrand H; Blasius B; Rabus R
[Ad] Endereço:General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), University Oldenburg, Oldenburg 26111, Germany.
[Ti] Título:Non-Redfield, nutrient synergy and flexible internal elemental stoichiometry in a marine bacterium.
[So] Source:FEMS Microbiol Ecol;93(5), 2017 May 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The stoichiometric constraints of algal growth are well understood, whereas there is less knowledge for heterotrophic bacterioplankton. Growth of the marine bacterium Phaeobacter inhibens DSM 17395, belonging to the globally distributed Roseobacter group, was studied across a wide concentration range of NH4+ and PO43-. The unique dataset covers 415 different concentration pairs, corresponding to 207 different molar N:P ratios (from 10-2 to 105). Maximal growth (by growth rate and biomass yield) was observed within a restricted concentration range at N:P ratios (∼50-120) markedly above Redfield. Experimentally determined growth parameters deviated to a large part from model predictions based on Liebig's law of the minimum, thus implicating synergistic co-limitation due to biochemical dependence of resources. Internal elemental ratios of P. inhibens varied with external nutrient supply within physiological constraints, thus adding to the growing evidence that aquatic bacteria can be flexible in their internal elemental composition. Taken together, the findings reported here revealed that P. inhibens is well adapted to fluctuating availability of inorganic N and P, expected to occur in its natural habitat (e.g. colonized algae, coastal areas). Moreover, this study suggests that elemental variability in bacterioplankton needs to be considered in the ecological stoichiometry of the oceans.
[Mh] Termos MeSH primário: Compostos de Amônio/farmacologia
Fosfatos/farmacologia
Roseobacter/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Biomassa
Ecossistema
Processos Heterotróficos
Oceanos e Mares
Roseobacter/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Phosphates)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix059


  3 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28371138
[Au] Autor:Muthusamy S; Lundin D; Mamede Branca RM; Baltar F; González JM; Lehtiö J; Pinhassi J
[Ad] Endereço:Centre for Ecology and Evolution in Microbial Model Systems - EEMiS, Linnaeus University, Kalmar, SE-39182, Sweden.
[Ti] Título:Comparative proteomics reveals signature metabolisms of exponentially growing and stationary phase marine bacteria.
[So] Source:Environ Microbiol;19(6):2301-2319, 2017 Jun.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Much of the phenotype of a microorganism consists of its repertoire of metabolisms and how and when its proteins are deployed under different growth conditions. Hence, analyses of protein expression could provide important understanding of how bacteria adapt to different environmental settings. To characterize the flexibility of proteomes of marine bacteria, we investigated protein profiles of three important marine bacterial lineages - Oceanospirillaceae (Neptuniibacter caesariensis strain MED92), Roseobacter (Phaeobacter sp. MED193) and Flavobacteria (Dokdonia sp. MED134) - during transition from exponential to stationary phase. As much as 59-80% of each species' total proteome was expressed. Moreover, all three bacteria profoundly altered their expressed proteomes during growth phase transition, from a dominance of proteins involved in translation to more diverse proteomes, with a striking appearance of enzymes involved in different nutrient-scavenging metabolisms. Whereas the three bacteria shared several overarching metabolic strategies, they differed in important details, including distinct expression patterns of membrane transporters and proteins in carbon and phosphorous metabolism and storage compounds. These differences can be seen as signature metabolisms - metabolisms specific for lineages. These findings suggest that quantitative proteomics can inform about the divergent ecological strategies of marine bacteria in adapting to changes in environmental conditions.
[Mh] Termos MeSH primário: Metabolismo dos Carboidratos/genética
Flavobacteriaceae/metabolismo
Oceanospirillaceae/metabolismo
Transporte Proteico/genética
Roseobacter/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Metabolismo dos Carboidratos/fisiologia
Carbono/metabolismo
Flavobacteriaceae/genética
Oceanospirillaceae/genética
Oceanospirillaceae/crescimento & desenvolvimento
Transporte Proteico/fisiologia
Proteoma/metabolismo
Proteômica
Roseobacter/genética
Roseobacter/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Proteome); 7440-44-0 (Carbon)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13725


  4 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28370995
[Au] Autor:Teira E; Hernando-Morales V; Guerrero-Feijóo E; Varela MM
[Ad] Endereço:Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, 36310, Vigo, Spain.
[Ti] Título:Leucine, starch and bicarbonate utilization by specific bacterial groups in surface shelf waters off Galicia (NW Spain).
[So] Source:Environ Microbiol;19(6):2379-2390, 2017 Jun.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The capability of different bacterial populations to degrade abundant polymers, such as algal-derived polysaccharides, or to utilize preferentially polymers over monomers, remains largely unknown. In this study, microautoradiography was combined with fluorescence in situ hybridization (MAR-FISH) to evaluate the ability of Bacteroidetes, SAR11, Roseobacter spp., Gammaproteobacteria and SAR86 cells to use bicarbonate, leucine and starch under natural light conditions at two locations in shelf surface waters off NW Spain. The percentage of cells incorporating bicarbonate was relatively high (mean 32% ± 4%) and was positively correlated with the intensity of solar radiation. The proportion of cells using starch (mean 56% ± 4%) or leucine (mean 47% ± 4%) was significantly higher than that using bicarbonate. On average, SAR11, Roseobacter spp. and Gammaproteobacteria showed a similarly high percentage of cells using leucine (47%-65% of hybridized cells) than using starch (51%-64% of hybridized cells), while Bacteroidetes and SAR86 cells preferentially used starch (53% of hybridized cells) over leucine (34%-40% of hybridized cells). We suggest that the great percentage of bacteria using starch is related to a high ambient availability of polymers associated to algal cell lysis, which, in turn, weakens the short-term coupling between phytoplankton release and bacterial production.
[Mh] Termos MeSH primário: Bacteroidetes/metabolismo
Bicarbonatos/metabolismo
Gammaproteobacteria/metabolismo
Leucina/metabolismo
Roseobacter/metabolismo
Amido/metabolismo
[Mh] Termos MeSH secundário: Autorradiografia
Bacteroidetes/genética
Gammaproteobacteria/genética
Hibridização in Situ Fluorescente
Fitoplâncton/metabolismo
Roseobacter/genética
Água do Mar/microbiologia
Espanha
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bicarbonates); 9005-25-8 (Starch); GMW67QNF9C (Leucine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13748


  5 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28171869
[Au] Autor:Ruppersberg HS; Goebel MR; Kleinert SI; Wünsch D; Trautwein K; Rabus R
[Ad] Endereço:General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
[Ti] Título:Photometric Determination of Ammonium and Phosphate in Seawater Medium Using a Microplate Reader.
[So] Source:J Mol Microbiol Biotechnol;27(2):73-80, 2017.
[Is] ISSN:1660-2412
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:To more efficiently process the large sample numbers for quantitative determination of ammonium (NH4+) and phosphate (orthophosphate, PO43-) generated during comprehensive growth experiments with the marine Roseobacter group member Phaeobacter inhibens DSM 17395, specific colorimetric assays employing a microplate reader (MPR) were established. The NH4+ assay is based on the reaction of NH4+ with hypochlorite and salicylate, yielding a limit of detection of 14 µM, a limit of quantitation of 36 µM, and a linear range for quantitative determination up to 200 µM. The PO43-assay is based on the complex formation of PO43- with ammonium molybdate in the presence of ascorbate and zinc acetate, yielding a limit of detection of 13 µM, a limit of quantitation of 50 µM, and a linear range for quantitative determination up to 1 mM. Both MPR-based assays allowed for fast (significantly lower than 1 h) analysis of 21 samples plus standards for calibration (all measured in triplicates) and showed only low variation across a large collection of biological samples.
[Mh] Termos MeSH primário: Compostos de Amônio/análise
Meios de Cultura/química
Fosfatos/análise
Fotometria/métodos
Água do Mar/química
[Mh] Termos MeSH secundário: Compostos de Amônio/química
Ácido Ascórbico/química
Ácido Hipocloroso/química
Molibdênio/química
Fosfatos/química
Fotometria/instrumentação
Roseobacter/crescimento & desenvolvimento
Roseobacter/metabolismo
Salicilatos/química
Sensibilidade e Especificidade
Estatística como Assunto
Acetato de Zinco/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Culture Media); 0 (Phosphates); 0 (Salicylates); 712K4CDC10 (Hypochlorous Acid); 81AH48963U (Molybdenum); FM5526K07A (Zinc Acetate); OGI154X474 (ammonium molybdate); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1159/000454814


  6 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28142225
[Au] Autor:Sun Y; Zhang Y; Hollibaugh JT; Luo H
[Ad] Endereço:Simon F. S. Li Marine Science Laboratory, School of Life Sciences and Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
[Ti] Título:Ecotype diversification of an abundant Roseobacter lineage.
[So] Source:Environ Microbiol;19(4):1625-1638, 2017 Apr.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Roseobacter DC5-80-3 cluster (also known as the RCA clade) is among the most abundant bacterial lineages in temperate and polar oceans. Previous studies revealed two phylotypes within this cluster that are distinctly distributed in the Antarctic and other ocean provinces. Here, we report a nearly complete genome co-assembly of three closely related single cells co-occurring in the Antarctic, and compare it to the available genomes of the other phylotype from ocean regions where iron is more accessible but phosphorus and nitrogen are less. The Antarctic phylotype exclusively contains an operon structure consisting of a dicitrate transporter fecBCDE and an upstream regulator likely for iron uptake, whereas the other phylotype consistently carry a high-affinity phosphate pst transporter and the phoB-phoR regulatory system, a high-affinity ammonium amtB transporter, urea and taurine utilization systems. Moreover, the Antarctic phylotype uses proteorhodopsin to acquire light, whereas the other uses bacteriochlorophyll-a and the sulfur-oxidizing sox cluster for energy acquisition. This is potentially an iron-saving strategy for the Antarctic phylotype because only the latter two pathways have iron-requiring cytochromes. Therefore, the two DC5-80-3 phylotypes, while diverging by only 1.1% in their 16S rRNA genes, have evolved systematic differences in metabolism to support their distinct ecologies.
[Mh] Termos MeSH primário: Ecótipo
Roseobacter/genética
[Mh] Termos MeSH secundário: Regiões Antárticas
Genoma Bacteriano
Oceanos e Mares
Filogenia
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13683


  7 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28075408
[Au] Autor:Alsufyani T; Weiss A; Wichard T
[Ad] Endereço:Institute for Inorganic and Analytical Chemistry, Jena School for Microbial Communication, Friedrich Schiller University Jena, Jena 07743, Germany. taghreed.alsufyani@gmail.com.
[Ti] Título:Time Course Exo-Metabolomic Profiling in the Green Marine Macroalga Ulva (Chlorophyta) for Identification of Growth Phase-Dependent Biomarkers.
[So] Source:Mar Drugs;15(1), 2017 Jan 10.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The marine green macroalga (Chlorophyta) lives in a mutualistic symbiosis with bacteria that influence growth, development, and morphogenesis. We surveyed changes in 's chemosphere, which was defined as a space where organisms interact with each other via compounds, such as infochemicals, nutrients, morphogens, and defense compounds. Thereby, cooperates with bacteria, in particular, sp. strain MS2 and sp. strain MS6 (formerly identified as sp. strain MS2 and sp. strain MS6). Without this accompanying microbial flora, forms only callus-like colonies. However, upon addition of the two bacteria species, in effect forming a tripartite community, morphogenesis can be completely restored. Under this strictly standardized condition, bioactive and eco-physiologically-relevant marine natural products can be discovered. Solid phase extracted waterborne metabolites were analyzed using a metabolomics platform, facilitating gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analysis, combined with the necessary acquisition of biological metadata. Multivariate statistics of the GC-MS and LC-MS data revealed strong differences between 's growth phases, as well as between the axenic cultures and the tripartite community. Waterborne biomarkers, including glycerol, were identified as potential indicators for algal carbon source and bacterial-algal interactions. Furthermore, it was demonstrated that releases glycerol that can be utilized for growth by sp. MS2.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Clorófitas/metabolismo
Ulva/metabolismo
[Mh] Termos MeSH secundário: Clorófitas/microbiologia
Cromatografia Gasosa-Espectrometria de Massas/métodos
Metabolômica/métodos
Morfogênese/fisiologia
Roseobacter/metabolismo
Alga Marinha/metabolismo
Alga Marinha/microbiologia
Ulva/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE


  8 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28040253
[Au] Autor:Díaz-García A; Borrero-Santiago AR; Ángel DelValls T; Riba I
[Ad] Endereço:UNESCO/UNITWIN Wicop, Departamento de Química-Física, Facultad de Ciencias Del Mar y Ambientales, Universidad de Cádiz, Polígono Río San Pedro s/n, Puerto Real, 11510 Cádiz, Spain. Electronic address: alejandradiazgt@gmail.com.
[Ti] Título:Simulating CO leakage from sub-seabed storage to determine metal toxicity on marine bacteria.
[So] Source:Mar Pollut Bull;116(1-2):80-86, 2017 Mar 15.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CO storage in sub-seabed marine geological formations has been proposed as an adequate strategy to mitigate high CO concentration from the atmosphere. The lack of knowledge about the potential risks of this technology on marine bacteria population in presence of metals has lead us to perform laboratory-scale experiments in order to evaluate its consequences. Thus, the effects of Zn and Cd were studied under acid conditions on Roseobacter sp. and Pseudomonas litoralis. Bacterial abundance (cellsmL ), growth rates (µ, h ), relative inhibitory effects of CO (RI ), and production of Extracellular Polysaccharides Substances (EPS) (µgGlucosecells ) were evaluated. A decreasing exopolysaccharides (EPS) production was found under low pH. Bacterial abundance as well as growth rates showed negative effects. Data obtained in this work are useful to determine the potential effects associated with enrichment of CO and metals on the marine ecosystem.
[Mh] Termos MeSH primário: Dióxido de Carbono/química
Sedimentos Geológicos/química
Metais Pesados/toxicidade
Pseudomonas/efeitos dos fármacos
Roseobacter/efeitos dos fármacos
Água do Mar/química
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Microbiologia da Água
Poluentes Químicos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Water Pollutants, Chemical); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  9 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27957709
[Au] Autor:Layer G; Krausze J; Moser J
[Ad] Endereço:Institut für Biochemie, Universität Leipzig, Brüderstraße 34, D-04103, Leipzig, Germany.
[Ti] Título:Reduction of Chemically Stable Multibonds: Nitrogenase-Like Biosynthesis of Tetrapyrroles.
[So] Source:Adv Exp Med Biol;925:147-161, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sophisticated biochemistry of nitrogenase plays a fundamental role for the biosynthesis of tetrapyrrole molecules, acting as key components of photosynthesis and methanogenesis. Three nitrogenase-like metalloenzymes have been characterized to date. Synthesis of chlorophylls and bacteriochlorophylls involves the reduction of the C17-C18 double bond of the conjugated ring system of protochlorophyllide which is catalyzed by the multi-subunit enzyme dark operative protochlorophyllide oxidoreductase (DPOR). Subsequently, biosynthesis of all bacteriochlorophylls requires the reduction of the C7-C8 double bond by a second nitrogenase-like enzyme termed chlorophyllide oxidoreductase (COR). Mechanistically, DPOR and COR make use of a reductase component which links ATP hydrolysis to conformational changes. This dynamic switch protein is triggering the transient association between the reductase and the core catalytic protein complex, thereby facilitating the transduction of electrons via two [4Fe4S] clusters. X-ray crystallographic structural investigations in combination with biochemical experiments revealed the molecular basis of the underlying energy transduction mechanism. The unique nickel-containing tetrapyrrole cofactor F is located in the active site of methyl-coenzyme M reductase, which is catalyzing the final step of methane formation in methanogenic archaea. The nitrogenase-like protein NflH/NflD has been proposed to catalyze one or more ring reduction steps during the biosynthesis of F . The present working hypothesis mirrors a DPOR and COR related enzyme mechanism of NflH/NflD. Furthermore, nfl-encoded proteins were suggested as "simplified" ancestors lying basal in the phylogenetic tree between nitrogenase and DPOR/COR.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Bacterioclorofilas/química
Nitrogenase/química
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/química
Oxirredutases/química
Tetrapirróis/química
[Mh] Termos MeSH secundário: Azotobacter vinelandii/genética
Azotobacter vinelandii/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Bacterioclorofilas/biossíntese
Biocatálise
Expressão Gênica
Nitrogenase/genética
Nitrogenase/metabolismo
Oxirredução
Oxirredutases/genética
Oxirredutases/metabolismo
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo
Fotossíntese/genética
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Estrutura Terciária de Proteína
Rhodobacter capsulatus/genética
Rhodobacter capsulatus/metabolismo
Roseobacter/genética
Roseobacter/metabolismo
Tetrapirróis/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacteriochlorophylls); 0 (Tetrapyrroles); EC 1.- (Oxidoreductases); EC 1.18.6.1 (Nitrogenase); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.- (chlorophyllide a reductase, Rhodobacter capsulatus); EC 1.3.1.33 (protochlorophyllide reductase); EC 2.8.4.1 (methyl coenzyme M reductase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1007/5584_2016_175


  10 / 212 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27552638
[Au] Autor:Sonnenschein EC; Nielsen KF; D'Alvise P; Porsby CH; Melchiorsen J; Heilmann J; Kalatzis PG; López-Pérez M; Bunk B; Spröer C; Middelboe M; Gram L
[Ad] Endereço:Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark.
[Ti] Título:Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis.
[So] Source:ISME J;11(2):569-583, 2017 Feb.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tropodithietic acid (TDA)-producing Ruegeria mobilis strains of the Roseobacter clade have primarily been isolated from marine aquaculture and have probiotic potential due to inhibition of fish pathogens. We hypothesized that TDA producers with additional novel features are present in the oceanic environment. We isolated 42 TDA-producing R. mobilis strains during a global marine research cruise. While highly similar on the 16S ribosomal RNA gene level (99-100% identity), the strains separated into four sub-clusters in a multilocus sequence analysis. They were further differentiated to the strain level by average nucleotide identity using pairwise genome comparison. The four sub-clusters could not be associated with a specific environmental niche, however, correlated with the pattern of sub-typing using co-isolated phages, the number of prophages in the genomes and the distribution in ocean provinces. Major genomic differences within the sub-clusters include prophages and toxin-antitoxin systems. In general, the genome of R. mobilis revealed adaptation to a particle-associated life style and querying TARA ocean data confirmed that R. mobilis is more abundant in the particle-associated fraction than in the free-living fraction occurring in 40% and 6% of the samples, respectively. Our data and the TARA data, although lacking sufficient data from the polar regions, demonstrate that R. mobilis is a globally distributed marine bacterial species found primarily in the upper open oceans. It has preserved key phenotypic behaviors such as the production of TDA, but contains diverse sub-clusters, which could provide new capabilities for utilization in aquaculture.
[Mh] Termos MeSH primário: Variação Genética
Genoma Bacteriano/genética
Rhodobacteraceae/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Geografia
Tipagem de Sequências Multilocus
Oceanos e Mares
Filogenia
Rhodobacteraceae/isolamento & purificação
Rhodobacteraceae/metabolismo
Roseobacter/genética
Roseobacter/isolamento & purificação
Roseobacter/metabolismo
Análise de Sequência de DNA
Tropolona/análogos & derivados
Tropolona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (tropodithietic acid); 7L6DL16P1T (Tropolone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160824
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.111



página 1 de 22 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde