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Referências encontradas : 259 [refinar]
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[PMID]:26666198
[Au] Autor:Chen Y; Xianyu Y; Sun J; Niu Y; Wang Y; Jiang X
[Ad] Endereço:Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China. xingyujiang@nanoctr.cn.
[Ti] Título:One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads.
[So] Source:Nanoscale;8(2):1100-7, 2016 Jan 14.
[Is] ISSN:2040-3372
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Ensaio de Imunoadsorção Enzimática/métodos
Separação Imunomagnética/métodos
Neoplasias/diagnóstico
[Mh] Termos MeSH secundário: Escherichia coli/isolamento & purificação
Seres Humanos
Magnetismo
Nanotecnologia/métodos
Neoplasias/genética
Óptica e Fotônica
Sistemas Automatizados de Assistência Junto ao Leito
Salmonella/isolamento & purificação
Shigella/isolamento & purificação
Spirillum/isolamento & purificação
Staphylococcus aureus/isolamento & purificação
Estresse Mecânico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers, Tumor)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151216
[St] Status:MEDLINE
[do] DOI:10.1039/c5nr07044a


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[PMID]:20178843
[Au] Autor:Zhu K; Pan H; Li J; Yu-Zhang K; Zhang SD; Zhang WY; Zhou K; Yue H; Pan Y; Xiao T; Wu LF
[Ad] Endereço:Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
[Ti] Título:Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea.
[So] Source:Res Microbiol;161(4):276-83, 2010 May.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats. Because of their fastidious requirements for growth conditions, only very few axenic MTB cultures have been obtained worldwide. In this study, we report a novel marine magnetotactic spirillum axenic culture, designated as QH-2, isolated from the China Sea. It was able to grow in semi-solid or liquid chemically defined medium. The cells were amphitrichously flagellated and contained one single magnetosome chain with an average number of 16 magnetosomes per cell. Phosphate and lipid granules were also observed in the cells. Both rock magnetism and energy-dispersive X-ray spectroscopy characterizations indicated that the magnetosomes in QH-2 were single-domain magnetites (Fe(3)O(4)). QH-2 cells swam mostly in a straight line at a velocity of 20-50 microm/s and occasionally changed to a helical motion. Unlike other magnetotactic spirilla, QH-2 cells responded to light illumination. As a consequence of illumination, the cells changed the direction in which they swam from parallel to the magnetic field to antiparallel. This response appears to be similar to the effect of an increase in [O(2)]. Analysis of the QH-2 16S rRNA sequence showed that it had greater than 11% sequence divergence from freshwater magnetotactic spirilla. Thus, the marine QH-2 strain seems to be both phylogenetically and magnetotactically distinct from the freshwater Magnetospirillum spp. studied previously.
[Mh] Termos MeSH primário: Magnetismo
Água do Mar/microbiologia
Spirillum/classificação
Spirillum/isolamento & purificação
[Mh] Termos MeSH secundário: China
Magnetossomos/química
Magnetossomos/genética
Magnetossomos/metabolismo
Dados de Sequência Molecular
Filogenia
Spirillum/química
Spirillum/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1008
[Cu] Atualização por classe:100601
[Lr] Data última revisão:
100601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100225
[St] Status:MEDLINE
[do] DOI:10.1016/j.resmic.2010.02.003


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[PMID]:19820156
[Au] Autor:Weinrich LA; Giraldo E; Lechevallier MW
[Ad] Endereço:American Water, Voorhees, NJ 08043, USA.
[Ti] Título:Development and application of a bioluminescence-based test for assimilable organic carbon in reclaimed waters.
[So] Source:Appl Environ Microbiol;75(23):7385-90, 2009 Dec.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Assimilable organic carbon (AOC) is an important parameter governing the growth of heterotrophic bacteria in drinking water. Despite the recognition that variations in treatment practices (e.g., disinfection, coagulation, selection of filter media, and watershed protection) can have dramatic impacts on AOC levels in drinking water, few water utilities routinely measure AOC levels because of the difficulty of the method. To simplify the method, the Pseudomonas fluorescens P-17 and Spirillum sp. strain NOX test bacteria were mutagenized by using luxCDABE operon fusion and inducible transposons to produce bioluminescent strains. The growth of these strains can easily be monitored with a programmable luminometer to determine the maximum cell yield via luminescence readings, and these values can be fitted to the classical Monod growth curve to determine bacterial growth kinetics and the maximum growth rate. Standard curves using acetate carbon (at concentrations ranging from 0 to 1,000 microg/liter) resulted in coefficients of determination (r(2)) between luminescence units and acetate carbon levels of 0.95 for P-17 and 0.89 for NOX. The bioluminescence test was used to monitor reclaimed water, in which average AOC levels range between 150 and 1,400 microg/liter acetate carbon equivalents. Comparison of the conventional AOC assay and the bioluminescent assay produced an r(2) of 0.92.
[Mh] Termos MeSH primário: Carbono/metabolismo
Compostos Orgânicos/análise
Pseudomonas fluorescens/metabolismo
Spirillum/metabolismo
Purificação da Água/métodos
Água/química
[Mh] Termos MeSH secundário: Genes Reporter
Luciferases/genética
Luciferases/metabolismo
Luminescência
Pseudomonas fluorescens/genética
Spirillum/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Organic Chemicals); 059QF0KO0R (Water); 7440-44-0 (Carbon); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1003
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091013
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01728-09


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[PMID]:19625415
[Au] Autor:Podkopaeva D; Grabovich M; Kuever J; Lysenko AM; Tourova TP; Kolganova TV; Dubinina G
[Ad] Endereço:Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl. 1, Voronezh 394006, Russia.
[Ti] Título:Proposal of Spirillum winogradskyi sp. nov., a novel microaerophilic species, an emended description of the genus Spirillum and Request for an Opinion regarding the status of the species Spirillum volutans Ehrenberg 1832.
[So] Source:Int J Syst Evol Microbiol;59(Pt 11):2916-20, 2009 Nov.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel obligately organotrophic, facultatively microaerophilic spirillum, designated strain D-427(T), was isolated from sulfidic sludge of a municipal wastewater-treatment plant. Cells were Gram-negative, large and highly motile due to bipolar tufts of flagella covered with mucous sheaths. Coccoid cells were sometimes formed. Strain D-427(T) grew optimally at pH 7.5-7.8 and 28 degrees C in the presence of 2 % O(2) in the gas phase. The organism showed oxidase and very low catalase activity. The isolate grew chemo-organotrophically with a limited number of organic acids as substrates. The DNA G+C content was 38.0 mol% (T(m)). Phylogenetic analysis of the 16S rRNA gene sequence placed strain D-427(T) in the genus Spirillum within the class Betaproteobacteria. The 16S rRNA gene sequence similarity between strain D-427(T) and Spirillum volutans ATCC 19554(T), the type strain of the single species of the genus, was 98.6 %. The low level of DNA-DNA hybridization and different phenotypic properties indicate that strain D-427(T) is clearly distinguishable from Spirillum volutans. No strain of S. volutans is available from any established culture collection or from the authors who described this species. Therefore, on the basis of phenotypic and genotypic data and the fact that the type and single species of the genus Spirillum cannot be included in any scientific study, since the type strain has been lost, we propose to assign strain D-427(T) as a novel species of the genus Spirillum, Spirillum winogradskyi sp. nov. (type strain D-427(T) =DSM 12756(T) =VKM B-2518(T)), and we request that the Judicial Commission place the name Spirillum volutans on the list of rejected names if a suitable type strain is not found or a neotype is not proposed within 2 years following the publication of this paper. An emended description of the genus Spirillum is also provided.
[Mh] Termos MeSH primário: Esgotos/microbiologia
Spirillum/classificação
Spirillum/isolamento & purificação
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
DNA Ribossômico/genética
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
Spirillum/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (Sewage)
[Em] Mês de entrada:0912
[Cu] Atualização por classe:091103
[Lr] Data última revisão:
091103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090724
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.009605-0


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[PMID]:19008054
[Au] Autor:Gaastra W; Boot R; Ho HT; Lipman LJ
[Ad] Endereço:Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
[Ti] Título:Rat bite fever.
[So] Source:Vet Microbiol;133(3):211-28, 2009 Jan 13.
[Is] ISSN:0378-1135
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rat bite fever (RBF) is a bacterial zoonosis for which two causal bacterial species have been identified: Streptobacillis moniliformis and Spirillum minus. Haverhill fever (HF) is a form of S. moniliformis infection believed to develop after ingestion of contaminated food or water. Here the infectious agents, their host species, pathogenicity (virulence factors and host susceptibility), diagnostic methods, therapy, epidemiology, transmission and prevention are described. Special emphasis is given on information from the field of laboratory animal microbiology and suggestions for future research.
[Mh] Termos MeSH primário: Febre por Mordedura de Rato/microbiologia
Spirillum
Streptobacillus
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:0904
[Cu] Atualização por classe:081216
[Lr] Data última revisão:
081216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:081115
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetmic.2008.09.079


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[PMID]:18788187
[Au] Autor:Abma R; Boer H; Douma G; van Hoeck B; Lipman L; Gaastra W
[Ad] Endereço:Universiteit Utrecht.
[Ti] Título:[What the practicing veterinarian should know about rat bite fever].
[Ti] Título:Wat de praktiserende dierenarts moet weten over rattenbeetziekte..
[So] Source:Tijdschr Diergeneeskd;133(16):668-70, 2008 Aug 15.
[Is] ISSN:0040-7453
[Cp] País de publicação:Netherlands
[La] Idioma:dut
[Mh] Termos MeSH primário: Mordeduras e Picadas/microbiologia
Febre por Mordedura de Rato/transmissão
Febre por Mordedura de Rato/veterinária
Doenças dos Roedores/transmissão
Zoonoses
[Mh] Termos MeSH secundário: Animais
Mordeduras e Picadas/veterinária
Seres Humanos
Doenças Profissionais
Febre por Mordedura de Rato/patologia
Febre por Mordedura de Rato/prevenção & controle
Ratos
Fatores de Risco
Spirillum/patogenicidade
Streptobacillus/patogenicidade
Médicos Veterinários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0810
[Cu] Atualização por classe:091111
[Lr] Data última revisão:
091111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080916
[St] Status:MEDLINE


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[PMID]:16758869
[Au] Autor:Podkopaeva DA; Grabovich MIu; Dubinina GA; Lysenko AM; Turova TP; Kolganova TV
[Ti] Título:[Two new species of microaerophilic sulfur spirilla, Spirillum winogradskii sp. nov. and Spirillum kriegii sp. nov].
[So] Source:Mikrobiologiia;75(2):212-20, 2006 Mar-Apr.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:New microaerophilic sulfur-oxidizing spirilla were isolated from hydrogen sulfide sludge of wastewater treatment plants. Strains D-427 and D-430 have spiral cells that are highly motile due to bipolar flagellum bundles covered with mucous sheaths. Under a phase-contrast microscope, these bundles are visible as single polar flagella. Spheroplasts are formed in the stationary growth phase. Both strains are obligate organotrophs able to oxidize a number of reduced sulfur compounds. The oxidation of sulfide and polysulfide leads to the formation of intracellular globules of elemental sulfur; thiosulfate oxidation results in tetrathionate accumulation in the medium. The cells are unable to utilize reduced sulfur compounds in the energy metabolism; their oxidation is caused by a chemical interaction with H2O2 and O2, synthesized in the electron transport chain. Both strains are obligate microaerophiles with an optimal oxygen concentration in the gas phase of 2 and 0.8% for strains D-427 and D-430, respectively. The strains utilize a limited number of organic acids as growth substrates, mainly tricarboxylic-acid-cycle intermediates. The DNA G+C content is 38.0 mol % (T(m)) for strain D-427 and 38.9 mol % for strain D-430. Phylogenetic analysis, based on the comparison of 16S rRNA gene sequences, revealed that the new isolates of sulfur spirilla are the most closely related to Spirillum volutans, the type species of the genus (97.4% similarity). They were assigned to the genus Spirillum within the class Beta-proteobacteria as two new species, S. winogradskii sp. nov. (D-427T = DSM 12756T) and S. kriegii sp. nov. (B-430T = BKM B-2372T). The emended description of the genus Spirillum is provided.
[Mh] Termos MeSH primário: Esgotos/microbiologia
Spirillum/metabolismo
Spirillum/ultraestrutura
Enxofre/metabolismo
Microbiologia da Água
[Mh] Termos MeSH secundário: Genótipo
Oxigênio/farmacologia
Filogenia
Spirillum/isolamento & purificação
Sulfetos/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sewage); 0 (Sulfides); 70FD1KFU70 (Sulfur); 9080-49-3 (polysulfide); S88TT14065 (Oxygen)
[Em] Mês de entrada:0607
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060609
[St] Status:MEDLINE


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[PMID]:16583822
[Au] Autor:Park SK; Lee SH; Choi SC; Kim YK
[Ad] Endereço:Department of Environmental Engineering, Kangwon National University, Hyoja 2-dong, Chunchon, Kangwondo 200-701, Korea.
[Ti] Título:Characteristics of biofilm community formed in the chlorinated biodegradable organic matter-limited tap water.
[So] Source:Environ Technol;27(4):377-86, 2006 Apr.
[Is] ISSN:0959-3330
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to characterize the influence of free chlorine residual on biofilm formation in a chlorinated system in which the biodegradable organic matter (BOM) was limited. The biofilm community was characterized through a community-level physiological profile (CLPP) that was generated using the Biolog GN microplate-based community-level assay. The chlorinated system was run at chlorine residual concentrations of 0.3, 0.5, and 1.0 mg l(-1) with the provision of BOM-limited tap water (0.01 mg l(-1) as assimilable organic carbon and 0.06 mg l(-1) as biodegradable dissolved organic carbon). For comparison, an unchlorinated system was operated in parallel under the same condition. The number of viable heterotrophic bacteria in the biofilm that formed in the chlorinated system over the 3 months of operation averaged 7.2 x 10(3), 4.8 x 10, and 1.6 x 10 CFU cm(-2) for the chlorine residual concentrations of 0.3, 0.5, and 1.0 mg l(-1), respectively. In the unchlorinated system, the average bacterial content was 1.1 x 10(6) CFU cm(-2). Using measures of substrate utilization rate, substrate utilization diversity, and metabolic potential index (MPI), the CLPP patterns demonstrated that the metabolic potentials of the biofilm communities decreased markedly as the chlorine residual levels increased. In particular, the community level of the biofilm that formed in the system with chlorine residual concentration of 1.0 mg l(-1) was the lowest of any biofilm under the tested conditions. The results implied that chlorine residual had a positive biocidal effect on the metabolic potential and/or functional potential of the biofilm community, especially when the BOM level was low. In addition, BOM limitation by itself was not sufficient to control biofilm formation.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Cloro/metabolismo
Compostos Orgânicos/metabolismo
Purificação da Água/métodos
Abastecimento de Água
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Contagem de Colônia Microbiana
Monitoramento Ambiental
Pseudomonas fluorescens
Spirillum
Temperatura Ambiente
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Organic Chemicals); 4R7X1O2820 (Chlorine)
[Em] Mês de entrada:0608
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060406
[St] Status:MEDLINE


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[PMID]:15835774
[Au] Autor:Podkopaeva DA; Grabovich MIu; Dubinina GA
[Ti] Título:[The functional role of reduced inorganic sulfur compounds in the metabolism of the microaerophilic bacterium Spirillum winogradskii].
[So] Source:Mikrobiologiia;74(1):17-25, 2005 Jan-Feb.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Oxidation of reduced sulfur compounds by microaerophilic sulfur bacterium Spirillum winogradskii was found to occur only concomitantly with consumption of an organic substrate and was not linked to their utilization as electron donors in energy metabolism. No enzymes of dissimilatory sulfur metabolism were found in the cells of the sulfur bacterium oxidizing thiosulfate to tetrathionate; oxidation of thiosulfate and sulfide was caused by their reaction with reactive oxygen species (ROS), mostly H2O2 produced in the course of aerobic growth. Decreased lytic effect of ROS in the presence of thiosulfate resulted in a twofold increase in the cell yield under aerobic conditions and more efficient substrate utilization. The latter effect was caused by decreased expense of energy for the biosynthesis of oxygen-protecting polysaccharides. The stimulatory effect of thiosulfate on the growth processes was due to the activation of a number of TCA cycle enzymes producing the intermediates for constructive metabolism, especially of the NADP-dependent malic enzyme. As a result of thiosulfate-induced synthesis of SH-containing cell components, the integral antioxidative activity increased 1.5-fold.
[Mh] Termos MeSH primário: Oxigênio/metabolismo
Spirillum/metabolismo
Compostos de Enxofre/metabolismo
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/biossíntese
Peróxido de Hidrogênio/metabolismo
Malato Desidrogenase/metabolismo
Oxirredução
Espécies Reativas de Oxigênio/metabolismo
Spirillum/crescimento & desenvolvimento
Tiossulfatos/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 0 (Sulfur Compounds); 0 (Thiosulfates); 8L70Q75FXE (Adenosine Triphosphate); BBX060AN9V (Hydrogen Peroxide); EC 1.1.1.37 (Malate Dehydrogenase); S88TT14065 (Oxygen)
[Em] Mês de entrada:0508
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050420
[St] Status:MEDLINE


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[PMID]:15819854
[Au] Autor:Franklin MP; McDonald IR; Bourne DG; Owens NJ; Upstill-Goddard RC; Murrell JC
[Ad] Endereço:Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
[Ti] Título:Bacterial diversity in the bacterioneuston (sea surface microlayer): the bacterioneuston through the looking glass.
[So] Source:Environ Microbiol;7(5):723-36, 2005 May.
[Is] ISSN:1462-2912
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The bacterioneuston is defined as the community of bacteria present within the neuston or sea surface microlayer. Bacteria within this layer were sampled using a membrane filter technique and bacterial diversity was compared with that in the underlying pelagic coastal seawater using molecular ecological techniques. 16S rRNA gene libraries of approximately 500 clones were constructed from both bacterioneuston and the pelagic water samples and representative clones from each library were sequenced for comparison of bacterial diversity. The bacterioneuston was found to have a significantly lower bacterial diversity than the pelagic seawater, with only nine clone types (ecotaxa) as opposed to 46 ecotaxa in the pelagic seawater library. Surprisingly, the bacterioneuston clone library was dominated by 16S rRNA gene sequences affiliated to two groups of organisms, Vibrio spp. which accounted for over 68% of clones and Pseudoalteromonas spp. accounting for 21% of the library. The dominance of these two 16S rRNA gene sequence types within the bacterioneuston clone library was confirmed in a subsequent gene probing experiment. 16S rRNA gene probes specific for these groups of bacteria were designed and used to probe new libraries of 1000 clones from both the bacterioneuston and pelagic seawater DNA samples. This revealed that 57% of clones from the bacterioneuston library hybridized to a Vibrio sp.-specific 16S rRNA gene probe and 32% hybridized to a Pseudoalteromonas sp.-specific 16S rRNA gene probe. In contrast, the pelagic seawater library resulted in only 13% and 8% of 16S rRNA gene clones hybridizing to the Vibrio sp. and Pseudoalteromonas sp. probes respectively. Results from this study suggest that the bacterioneuston contains a distinct population of bacteria and warrants further detailed study at the molecular level.
[Mh] Termos MeSH primário: Bactérias Gram-Negativas/isolamento & purificação
Água do Mar/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Aeromonas/genética
Aeromonas/isolamento & purificação
Sequência de Bases
DNA Bacteriano/química
DNA Bacteriano/genética
Biblioteca Gênica
Variação Genética
Bactérias Gram-Negativas/genética
Dados de Sequência Molecular
Mar do Norte
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Pseudoalteromonas/genética
Pseudoalteromonas/isolamento & purificação
Pseudomonas/genética
Pseudomonas/isolamento & purificação
RNA Ribossômico 16S/química
RNA Ribossômico 16S/genética
Alinhamento de Sequência
Spirillum/genética
Spirillum/isolamento & purificação
Vibrio/genética
Vibrio/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0506
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050412
[St] Status:MEDLINE



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