Base de dados : MEDLINE
Pesquisa : B03.440.860 [Categoria DeCS]
Referências encontradas : 172 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 18 ir para página                         

  1 / 172 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28591831
[Au] Autor:Blasco G; Moreno-Navarrete JM; Rivero M; Pérez-Brocal V; Garre-Olmo J; Puig J; Daunis-I-Estadella P; Biarnés C; Gich J; Fernández-Aranda F; Alberich-Bayarri Á; Moya A; Pedraza S; Ricart W; López M; Portero-Otin M; Fernandez-Real JM
[Ad] Endereço:Research Unit, Institute of Diagnostic Imaging (IDI), Parc Sanitari Pere Virgili, Barcelona 08023, Spain.
[Ti] Título:The Gut Metagenome Changes in Parallel to Waist Circumference, Brain Iron Deposition, and Cognitive Function.
[So] Source:J Clin Endocrinol Metab;102(8):2962-2973, 2017 Aug 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: Microbiota perturbations seem to exert modulatory effects on emotional behavior, stress-, and pain-modulation systems in adult animals; however, limited information is available in humans. Objective: To study potential relationships among the gut metagenome, brain microstructure, and cognitive performance in middle-aged, apparently healthy, obese and nonobese subjects after weight changes. Design: This is a longitudinal study over a 2-year period. Setting: A tertiary public hospital. Patients or Other Participants: Thirty-five (18 obese) apparently healthy subjects. Intervention(s): Diet counseling was provided to all subjects. Obese subjects were followed every 6 months. Main Outcome Measure(s): Brain relaxometry (using magnetic resonance R2*), cognitive performance (by means of cognitive tests), and gut microbiome composition (shotgun). Results: R2* increased in both obese and nonobese subjects, independent of weight variations. Changes in waist circumference, but not in body mass index, were associated with brain iron deposition (R2*) in the striatum, amygdala, and hippocampus in parallel to visual-spatial constructional ability and circulating beta amyloid Aß42 levels. These changes were linked to shifts in gut microbiome in which the relative abundance of bacteria belonging to Caldiserica and Thermodesulfobacteria phyla were reciprocally associated with raised R2* in different brain nuclei. Of note, the increase in bacteria belonging to Tenericutes phylum was parallel to decreased R2* gain in the striatum, serum Aß42 levels, and spared visual-spatial constructional ability. Interestingly, metagenome functions associated with circulating and brain iron stores are involved in bacterial generation of siderophores. Conclusions: Changes in the gut metagenome are associated longitudinally with cognitive function and brain iron deposition.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Cognição
Microbioma Gastrointestinal/genética
Ferro/metabolismo
Metagenoma/genética
Obesidade/microbiologia
Circunferência da Cintura
[Mh] Termos MeSH secundário: Adulto
Bacteroidetes
Encéfalo/diagnóstico por imagem
Estudos de Casos e Controles
Estudos Transversais
Feminino
Firmicutes
Seres Humanos
Processamento de Imagem Assistida por Computador
Estudos Longitudinais
Imagem por Ressonância Magnética
Masculino
Meia-Idade
Testes Neuropsicológicos
Obesidade/metabolismo
Obesidade/psicologia
Tenericutes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
E1UOL152H7 (Iron)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00133


  2 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28100298
[Au] Autor:Cox LM; Sohn J; Tyrrell KL; Citron DM; Lawson PA; Patel NB; Iizumi T; Perez-Perez GI; Goldstein EJC; Blaser MJ
[Ad] Endereço:2​Department of Microbiology, NYU Langone Medical Center, New York, NY 10016, USA 1​Department of Medicine, NYU Langone Medical Center, New York, NY 10016, USA 3​Department of Neurology, Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA
[Ti] Título:Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. nov., sp. nov. and Dubosiella newyorkensis, gen. nov., sp. nov., from the murine intestine, and emendation to the description of Faecalibaculum rodentium.
[So] Source:Int J Syst Evol Microbiol;67(5):1247-1254, 2017 05.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoid-shaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene sequence similarity values of 87.7 %. A second group, represented by NYU-BL-A4T, was most closely related to Faecalibaculum rodentium, with 86.6 % 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9 %) compared with the recently described Faecalibaculum rodentium, also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (>10 %) cellular fatty acids of strain NYU-BL-A3T were C16 : 0 and C18 : 0, and those of strain NYU-BL-A4T were C10 : 0, C16 : 0, C18 : 0 and C18 : 1ω9c. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4T being considerably more active. Based on phylogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, Ileibacterium valens gen. nov., sp. nov. with NYU-BL-A3T (=ATCC TSD-63T=DSM 103668T) as the type strain and Dubosiella newyorkensis gen. nov., sp. nov. with NYU-BL-A4T (=ATCC TSD-64T=DSM 103457T) as the type strain.
[Mh] Termos MeSH primário: Faecalibacterium/classificação
Intestinos/microbiologia
Camundongos/microbiologia
Filogenia
Tenericutes/classificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tenericutes/genética
Tenericutes/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001793


  3 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28073398
[Au] Autor:Naito M; Desirò A; González JB; Tao G; Morton JB; Bonfante P; Pawlowska TE
[Ad] Endereço:1​Graduate Field of Microbiology, Cornell University, Ithaca, NY, USA.
[Ti] Título:'Candidatus Moeniiplasma glomeromycotorum', an endobacterium of arbuscular mycorrhizal fungi.
[So] Source:Int J Syst Evol Microbiol;67(5):1177-1184, 2017 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arbuscular mycorrhizal fungi (AMF, subphylum Glomeromycotina) are symbionts of most terrestrial plants. They commonly harbour endobacteria of a largely unknown biology, referred to as MRE (Mollicutes/mycoplasma-related endobacteria). Here, we propose to accommodate MRE in the novel genus 'Candidatus Moeniiplasma.' Phylogeny reconstructions based on the 16S rRNA gene sequences cluster 'Ca.Moeniiplasma' with representatives of the class Mollicutes, whereas phylogenies derived from amino acid sequences of 19 genes indicate that it is a discrete lineage sharing ancestry with the members of the family Mycoplasmataceae. Cells of 'Ca.Moeniiplasma' reside directly in the host cytoplasm and have not yet been cultivated. They are coccoid, ~500 nm in diameter, with an electron-dense layer outside the plasma membrane. However, the draft genomes of 'Ca.Moeniiplasma' suggest that this structure is not a Gram-positive cell wall. The evolution of 'Ca.Moeniiplasma' appears to be driven by an ultrarapid rate of mutation accumulation related to the loss of DNA repair mechanisms. Moreover, molecular evolution patterns suggest that, in addition to vertical transmission, 'Ca.Moeniiplasma' is able to transmit horizontally among distinct Glomeromycotina host lineages and exchange genes. On the basis of these unique lifestyle features, the new species 'Candidatus Moeniiplasma glomeromycotorum' is proposed.
[Mh] Termos MeSH primário: Micorrizas
Filogenia
Simbiose
Tenericutes/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Evolução Molecular
Plantas/microbiologia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tenericutes/genética
Tenericutes/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001785


  4 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28045919
[Au] Autor:Lindheim L; Bashir M; Münzker J; Trummer C; Zachhuber V; Leber B; Horvath A; Pieber TR; Gorkiewicz G; Stadlbauer V; Obermayer-Pietsch B
[Ad] Endereço:Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University Graz, Graz, Austria.
[Ti] Título:Alterations in Gut Microbiome Composition and Barrier Function Are Associated with Reproductive and Metabolic Defects in Women with Polycystic Ovary Syndrome (PCOS): A Pilot Study.
[So] Source:PLoS One;12(1):e0168390, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Polycystic ovary syndrome (PCOS) is a common female endocrinopathy of unclear origin characterized by hyperandrogenism, oligo-/anovulation, and ovarian cysts. Women with PCOS frequently display overweight, insulin resistance, and systemic low-grade inflammation. We hypothesized that endotoxemia resulting from a leaky gut is associated with inflammation, insulin resistance, fat accumulation, and hyperandrogenemia in PCOS. In this pilot study, we compared the stool microbiome, gut permeability, and inflammatory status of women with PCOS and healthy controls. METHODS: 16S rRNA gene amplicon sequencing was performed on stool samples from 24 PCOS patients and 19 healthy controls. Data processing and microbiome analysis were conducted in mothur and QIIME using different relative abundance cut-offs. Gut barrier integrity, endotoxemia, and inflammatory status were evaluated using serum and stool markers and associations with reproductive, metabolic, and anthropometric parameters were investigated. RESULTS: The stool microbiome of PCOS patients showed a lower diversity and an altered phylogenetic composition compared to controls. We did not observe significant differences in any taxa with a relative abundance>1%. When looking at rare taxa, the relative abundance of bacteria from the phylum Tenericutes, the order ML615J-28 (phylum Tenericutes) and the family S24-7 (phylum Bacteroidetes) was significantly lower and associated with reproductive parameters in PCOS patients. Patients showed alterations in some, but not all markers of gut barrier function and endotoxemia. CONCLUSION: Patients with PCOS have a lower diversity and an altered phylogenetic profile in their stool microbiome, which is associated with clinical parameters. Gut barrier dysfunction and endotoxemia were not driving factors in this patient cohort, but may contribute to the clinical phenotype in certain PCOS patients.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal
Síndrome do Ovário Policístico/microbiologia
[Mh] Termos MeSH secundário: Adulto
Antropometria
Bacteroidetes/classificação
Bacteroidetes/isolamento & purificação
Estudos de Casos e Controles
Fezes/microbiologia
Feminino
Seres Humanos
Inflamação
Projetos Piloto
Síndrome do Ovário Policístico/metabolismo
Análise de Componente Principal
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase em Tempo Real
Inquéritos e Questionários
Tenericutes/classificação
Tenericutes/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0168390


  5 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27865739
[Au] Autor:Totten AH; Xiao L; Crabb DM; Ratliff AE; Dybvig K; Waites KB; Atkinson TP
[Ad] Endereço:Dept of Pediatrics, 1720 2nd Ave South, Shelby 472, University of Alabama at Birmingham (UAB), Birmingham, AL 35294, USA. Electronic address: ahtotten@uab.edu.
[Ti] Título:Shaken or stirred?: Comparison of methods for dispersion of Mycoplasma pneumoniae aggregates for persistence in vivo.
[So] Source:J Microbiol Methods;132:56-62, 2017 Jan.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mycoplasma pneumoniae (Mpn), one of the smallest self-replicating prokaryotes, is known to readily adhere to host cells and to form aggregates in suspension. Having only one cell membrane and no cell wall, mycoplasmas present questions as to optimal aggregate disruption method while minimizing cell death in vitro. We compared conventional vortex mixing with other methods for disruption of bacterial aggregates and for its effect on cell viability. METHODS: Strain UAB PO1, a clinical Mpn isolate, was dispersed using a conventional vortex mixer with or without nonionic detergent (0.1% and 0.01% Tween-20), a probe-type ultrasonicator, or repeated passage through a 27-gauge needle. The resulting suspensions were assayed for recoverable colony-forming units (CFU). Flow cytometric assays were carried out to examine particle size and membrane integrity with the transmembrane potential dye DiBAC . Wet Scanning Transmission Electron Microscopy (Wet-STEM) was performed for high resolution imaging of the resultant cell suspensions. Additional Mpn strains and other human mollicute species were assayed in a similar manner. Mice were infected with either vortexed or sonicated UAB PO1 and bacterial persistence was examined via Mpn-specific 16S qPCR. RESULTS: Comparison between dispersion methods showed a 10-fold enrichment of recoverable Mpn CFU with sonication compared to other methods. Time-course analysis showed significantly lower bacterial CFU with vortexing compared to sonication at all time points. Flow cytometric analysis showed increased cellular membrane damage via DiBAC staining in sonicated suspensions, but a decreased particle size. Wet-STEM imaging showed markedly improved dispersion with sonication compared to conventional vortex treatment, and surprisingly vortexing for 30s produced up to a 100-fold drop in CFU. Results similar to UAB PO1 were obtained with three additional Mpn strains and other Mollicutes species, although they exhibited differential susceptibilities to disaggregation by sonication. Finally, increased persistence of the organism in a mouse model of infection was observed using sonicated suspensions for initial infection. CONCLUSIONS: Sonication is superior to vortexing with or without nonionic detergent or repeated 27-gauge needle passage for dispersion of Mpn aggregates while preserving cell viability. Preparation of Mpn suspensions for in vivo experiments is best accomplished using brief sonication due to the dramatic increase in CFU produced by sonication. Dispersion methods may affect the final experimental results and should be an important consideration for future research involving mycoplasma species.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas
Mycoplasma pneumoniae/isolamento & purificação
RNA Bacteriano/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Aderência Bacteriana
Contagem de Colônia Microbiana
Modelos Animais de Doenças
Feminino
Interações Hospedeiro-Patógeno
Pulmão/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Viabilidade Microbiana
Microscopia Eletrônica de Transmissão e Varredura
Mycoplasma pneumoniae/classificação
Tamanho da Partícula
Pneumonia por Mycoplasma/diagnóstico
Pneumonia por Mycoplasma/microbiologia
RNA Bacteriano/genética
Sonicação
Tenericutes/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE


  6 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27620272
[Au] Autor:Killiny N
[Ad] Endereço:Department of Plant Pathology, Citrus Research and Education Center, University of Florida, 700 Experiment Station Rd., Lake Alfred, FL 33850, USA. Electronic address: nabilkilliny@ufl.edu.
[Ti] Título:Generous hosts: What makes Madagascar periwinkle (Catharanthus roseus) the perfect experimental host plant for fastidious bacteria?
[So] Source:Plant Physiol Biochem;109:28-35, 2016 Dec.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Although much attention has been paid to the metabolism and biosynthesis of monoterpene alkaloids in Catharanthus roseus, its value as an experimental host for a variety of agriculturally and economically important phytopathogenic bacteria warrants further study. In the present study, we evaluated the chemical composition of the phloem and xylem saps of C. roseus to infer the nutritional requirements of phloem- and xylem-limited phytopathogens. Periwinkle phloem sap consisted of a rich mixture of sugars, organic acids, amino acids, amines, fatty acids, sugar acids and sugar alcohols while xylem contained similar compounds in lesser concentrations. Plant sap analysis may lead to a better understanding of the biology of fastidious Mollicutes and their complex nutritional requirements, and to successful culture of phytoplasmas and other uncultured phloem-restricted bacteria such as Candidatus Liberibacter asiaticus, the causal agent of huanglongbing in citrus.
[Mh] Termos MeSH primário: Bactérias/crescimento & desenvolvimento
Catharanthus/microbiologia
Floema/microbiologia
Xilema/microbiologia
[Mh] Termos MeSH secundário: Aminas/análise
Aminoácidos/análise
Bactérias/classificação
Produtos Biológicos/química
Produtos Biológicos/metabolismo
Carboidratos/análise
Catharanthus/metabolismo
Ácidos Graxos/análise
Cromatografia Gasosa-Espectrometria de Massas
Interações Hospedeiro-Patógeno
Concentração de Íons de Hidrogênio
Floema/metabolismo
Phytoplasma/fisiologia
Doenças das Plantas/microbiologia
Álcoois Açúcares/análise
Tenericutes/fisiologia
Xilema/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Amino Acids); 0 (Biological Products); 0 (Carbohydrates); 0 (Fatty Acids); 0 (Sugar Alcohols)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


  7 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27392436
[Au] Autor:Zhang W; Guo R; Yang Y; Ding J; Zhang Y
[Ad] Endereço:Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, PR China.
[Ti] Título:Long-term effect of heavy-metal pollution on diversity of gastrointestinal microbial community of Bufo raddei.
[So] Source:Toxicol Lett;258:192-197, 2016 Sep 06.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gastrointestinal (GI) microbiota plays a very important role in maintaining its host's health. However, the effects of environmental contamination on the GI microbiota homeostasis of amphibians have not yet been reported. The present study reveals the long-term effect of natural heavy-metal pollution on the GI microbial community diversity and structural changes of Bufo raddei (B. raddei). Basing on the 16S rRNA sequencing method, the GI microbiota of B. raddei from a heavily heavy-metal-polluted area (Baiyin, (BY)) and a relatively unpolluted area (Liujiaxia, (LJX)) were profiled. The results showed that heavy-metal pollution had caused significant shifts in the composition of the GI microbiota both at the phylum and genus levels. Specifically, Bacteroidetes dominated in the GI tract of B. raddei from BY, while Tenericutes was much more common in those from LJX. The ratio of Firmicutes/Bacteroidetes and the proportion of probiotics in the GI microbiota of B. raddei from BY were reduced compared to those from LJX, as well. Heavy-metal pollution also induced in a reduction of species diversity and decreased proportion of unique operational taxonomic units in the GI tract. In short, our results demonstrate that long-term heavy-metal exposure re-shaped the composition and decreased the species diversity of GI microbiota of B. raddei; our results also represent a novel approach to uncover the toxic effects of pollution on amphibians.
[Mh] Termos MeSH primário: Bufonidae/microbiologia
Disbiose/veterinária
Doença Ambiental/veterinária
Poluentes Ambientais/toxicidade
Microbioma Gastrointestinal/efeitos dos fármacos
Metais Pesados/toxicidade
[Mh] Termos MeSH secundário: Animais
Bacteroidetes/classificação
Bacteroidetes/efeitos dos fármacos
Bacteroidetes/crescimento & desenvolvimento
Cádmio/análise
Cádmio/toxicidade
China
Cobre/análise
Cobre/toxicidade
Disbiose/induzido quimicamente
Disbiose/microbiologia
Doença Ambiental/induzido quimicamente
Doença Ambiental/microbiologia
Poluentes Ambientais/análise
Trato Gastrointestinal/química
Trato Gastrointestinal/microbiologia
Chumbo/análise
Chumbo/toxicidade
Masculino
Metais Pesados/análise
Tipagem Molecular
Tanques/análise
Solo/química
Espectrofotometria Atômica
Tenericutes/classificação
Tenericutes/efeitos dos fármacos
Tenericutes/crescimento & desenvolvimento
Zonas Úmidas
Zinco/análise
Zinco/toxicidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Metals, Heavy); 0 (Soil); 00BH33GNGH (Cadmium); 2P299V784P (Lead); 789U1901C5 (Copper); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160710
[St] Status:MEDLINE


  8 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27258373
[Au] Autor:Niu Y; Meng Q; Li S; Ren L; Zhou B; Schonewille T; Zhou Z
[Ad] Endereço:State Key Laboratory of Animal Nutrition, Beijing, 100193, P. R. China.
[Ti] Título:Effects of Diets Supplemented with Ensiled Mulberry Leaves and Sun-Dried Mulberry Fruit Pomace on the Ruminal Bacterial and Archaeal Community Composition of Finishing Steers.
[So] Source:PLoS One;11(6):e0156836, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the effects of ensiled mulberry leaves (EML) and sun-dried mulberry fruit pomace (SMFP) on the ruminal bacterial and archaeal community composition of finishing steers. Corn grain- and cotton meal-based concentrate was partially replaced with EML or SMFP. The diets had similar crude protein (CP), neutral detergent fiber (NDF), and metabolizable energy. Following the feeding trial, the steers were slaughtered and ruminal liquid samples were collected to study the ruminal microbiome. Extraction of DNA, amplification of the V4 region of the 16S rRNA gene, and Illumina MiSeq pyrosequencing were performed for each sample. Following sequence de-noising, chimera checking, and quality trimming, an average of 209,610 sequences were generated per sample. Quantitative real-time PCR was performed to examine the selected bacterial species in the rumen. Our results showed that the predominant phyla were Bacteroidetes (43.90%), Firmicutes (39.06%), Proteobacteria (4.31%), and Tenericutes (2.04%), and the predominant genera included Prevotella (13.82%), Ruminococcus (2.51%), Butyrivibrio (2.38%), and Succiniclasticum (2.26%). Compared to the control group, EML and SMFP groups had a higher abundance of total bacteria (p < 0.001); however, the bacterial community composition was similar among the three groups. At the phylum level, there were no significant differences in Firmicutes (p = 0.7932), Bacteroidetes (p = 0.2330), Tenericutes (p = 0.2811), or Proteobacteria (p = 0.0680) levels among the three groups; however, Fibrobacteres decreased in EML (p = 0.0431). At the genus level, there were no differences in Prevotella (p = 0.4280), Ruminococcus (p = 0.2639), Butyrivibrio (p = 0.4433), or Succiniclasticum (p = 0.0431) levels among the groups. Additionally, the dietary treatments had no significant effects on the archaeal community composition in the rumen. Therefore, EML and SMFP supplementation had no significant effects on the ruminal bacterial or archaeal community composition of finishing steers.
[Mh] Termos MeSH primário: Archaea/efeitos dos fármacos
Bactérias/efeitos dos fármacos
Dieta/veterinária
Microbioma Gastrointestinal/efeitos dos fármacos
Morus/química
Extratos Vegetais/farmacologia
Rúmen/microbiologia
[Mh] Termos MeSH secundário: Animais
Archaea/isolamento & purificação
Bactérias/isolamento & purificação
Bacteroidetes/efeitos dos fármacos
Bacteroidetes/isolamento & purificação
Butyrivibrio/efeitos dos fármacos
Butyrivibrio/isolamento & purificação
Bovinos
Firmicutes/efeitos dos fármacos
Firmicutes/isolamento & purificação
Frutas/química
Folhas de Planta/química
Prevotella/efeitos dos fármacos
Prevotella/isolamento & purificação
Proteobactérias/efeitos dos fármacos
Proteobactérias/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Ruminococcus/efeitos dos fármacos
Ruminococcus/isolamento & purificação
Tenericutes/efeitos dos fármacos
Tenericutes/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0156836


  9 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27067447
[Au] Autor:Salling HK; Bang-Christensen SR
[Ad] Endereço:Novo Nordisk A/S, Biopharm Purification Development & Virology, Hagedornsvej 1, 2820 Gentofte, Denmark. Electronic address: HNSI@novonordisk.com.
[Ti] Título:Multi-primer qPCR assay capable of highly efficient and specific detection of the vast majority of all known Mycoplasma.
[So] Source:Biologicals;44(3):129-38, 2016 May.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mycoplasma bacteria are able to pass through sterilizing grade filters due to their small size and lack of a cell wall, making them a common contaminant of biopharmaceutical productions. The classical method for detecting Mycoplasma is described in the European Pharmacopeia (Ph.Eur) 2.6.7. The method takes 28 days to perform, due to the slow growing nature of some Mycoplasma species. The Ph.Eur has described Nucleic Acid Testing (NAT) as a rapid alternative to the classical method. Here we present the development of a quantitative polymerase chain reaction (qPCR) assay capable of unambiguous detection of Mycoplasma with high sensitivity and specificity. The broadness of detection and the specificity towards Mycoplasma has been investigated by in silico analysis of the primer sequences followed by testing on purified Mycoplasma DNA as well as DNA from closely related genera. The assay will in all probability detect at least 356 species and strains of Mycoplasma, Spiroplasma and Acholeplasma with high sensitivity. To our knowledge this assay has the most uniform amplification efficiency over the broadest range of species and it is extremely specific towards Mycoplasma. With appropriate validation, the assay can be applied as a powerful tool for rapid Mycoplasma detection in the biopharmaceutical industry.
[Mh] Termos MeSH primário: Acholeplasma/genética
DNA Bacteriano/genética
Mycoplasma/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
Spiroplasma/genética
[Mh] Termos MeSH secundário: Primers do DNA/genética
Contaminação de Medicamentos/prevenção & controle
Seres Humanos
Mycoplasma/classificação
RNA Ribossômico 16S/genética
Reprodutibilidade dos Testes
Tecnologia Farmacêutica/métodos
Tenericutes/classificação
Tenericutes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160413
[St] Status:MEDLINE


  10 / 172 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27058507
[Au] Autor:Skennerton CT; Haroon MF; Briegel A; Shi J; Jensen GJ; Tyson GW; Orphan VJ
[Ad] Endereço:Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, USA.
[Ti] Título:Phylogenomic analysis of Candidatus 'Izimaplasma' species: free-living representatives from a Tenericutes clade found in methane seeps.
[So] Source:ISME J;10(11):2679-2692, 2016 11.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tenericutes are a unique class of bacteria that lack a cell wall and are typically parasites or commensals of eukaryotic hosts. Environmental 16S rDNA surveys have identified a number of tenericute clades in diverse environments, introducing the possibility that these Tenericutes may represent non-host-associated, free-living microorganisms. Metagenomic sequencing of deep-sea methane seep sediments resulted in the assembly of two genomes from a Tenericutes-affiliated clade currently known as 'NB1-n' (SILVA taxonomy) or 'RF3' (Greengenes taxonomy). Metabolic reconstruction revealed that, like cultured members of the Mollicutes, these 'NB1-n' representatives lack a tricarboxylic acid cycle and instead use anaerobic fermentation of simple sugars for substrate level phosphorylation. Notably, the genomes also contained a number of unique metabolic features including hydrogenases and a simplified electron transport chain containing an RNF complex, cytochrome bd oxidase and complex I. On the basis of the metabolic potential predicted from the annotated genomes, we devised an anaerobic enrichment media that stimulated the growth of these Tenericutes at 10 °C, resulting in a mixed culture where these organisms represented ~60% of the total cells by targeted fluorescence in situ hybridization (FISH). Visual identification by FISH confirmed these organisms were not directly associated with Eukaryotes and electron cryomicroscopy of cells in the enrichment culture confirmed an ultrastructure consistent with the defining phenotypic property of Tenericutes, with a single membrane and no cell wall. On the basis of their unique gene content, phylogenetic placement and ultrastructure, we propose these organisms represent a novel class within the Tenericutes, and suggest the names Candidatus 'Izimaplasma sp. HR1' and Candidatus 'Izimaplasma sp. HR2' for the two genome representatives.
[Mh] Termos MeSH primário: Metano/metabolismo
Filogenia
Água do Mar/microbiologia
Tenericutes/genética
[Mh] Termos MeSH secundário: DNA Bacteriano/genética
Genoma Bacteriano
Genômica
Hibridização in Situ Fluorescente
Metano/análise
RNA Ribossômico 16S/genética
Água do Mar/análise
Tenericutes/classificação
Tenericutes/isolamento & purificação
Tenericutes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171127
[Lr] Data última revisão:
171127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160409
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.55



página 1 de 18 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde