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Pesquisa : B03.440.860.074 [Categoria DeCS]
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[PMID]:26223451
[Au] Autor:Quaglino F; Kube M; Jawhari M; Abou-Jawdah Y; Siewert C; Choueiri E; Sobh H; Casati P; Tedeschi R; Lova MM; Alma A; Bianco PA
[Ad] Endereço:Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, via Celoria 2, 20133, Milan, Italy. fabio.quaglino@unimi.it.
[Ti] Título:'Candidatus Phytoplasma phoenicium' associated with almond witches'-broom disease: from draft genome to genetic diversity among strain populations.
[So] Source:BMC Microbiol;15:148, 2015 Jul 30.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Almond witches'-broom (AlmWB), a devastating disease of almond, peach and nectarine in Lebanon, is associated with 'Candidatus Phytoplasma phoenicium'. In the present study, we generated a draft genome sequence of 'Ca. P. phoenicium' strain SA213, representative of phytoplasma strain populations from different host plants, and determined the genetic diversity among phytoplasma strain populations by phylogenetic analyses of 16S rRNA, groEL, tufB and inmp gene sequences. RESULTS: Sequence-based typing and phylogenetic analysis of the gene inmp, coding an integral membrane protein, distinguished AlmWB-associated phytoplasma strains originating from diverse host plants, whereas their 16S rRNA, tufB and groEL genes shared 100 % sequence identity. Moreover, dN/dS analysis indicated positive selection acting on inmp gene. Additionally, the analysis of 'Ca. P. phoenicium' draft genome revealed the presence of integral membrane proteins and effector-like proteins and potential candidates for interaction with hosts. One of the integral membrane proteins was predicted as BI-1, an inhibitor of apoptosis-promoting Bax factor. Bioinformatics analyses revealed the presence of putative BI-1 in draft and complete genomes of other 'Ca. Phytoplasma' species. CONCLUSION: The genetic diversity within 'Ca. P. phoenicium' strain populations in Lebanon suggested that AlmWB disease could be associated with phytoplasma strains derived from the adaptation of an original strain to diverse hosts. Moreover, the identification of a putative inhibitor of apoptosis-promoting Bax factor (BI-1) in 'Ca. P. phoenicium' draft genome and within genomes of other 'Ca. Phytoplasma' species suggested its potential role as a phytoplasma fitness-increasing factor by modification of the host-defense response.
[Mh] Termos MeSH primário: Variação Genética
Genoma Bacteriano
Phytoplasma/classificação
Phytoplasma/isolamento & purificação
Doenças das Plantas/microbiologia
Prunus dulcis/microbiologia
[Mh] Termos MeSH secundário: Acholeplasmataceae
Proteínas de Bactérias/genética
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Genótipo
Líbano
Dados de Sequência Molecular
Tipagem Molecular
Filogenia
Prunus persica/microbiologia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150802
[Lr] Data última revisão:
150802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150731
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-015-0487-4


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[PMID]:24158107
[Au] Autor:Kube M; Siewert C; Migdoll AM; Duduk B; Holz S; Rabus R; Seemüller E; Mitrovic J; Müller I; Büttner C; Reinhardt R
[Ad] Endereço:Division Phytomedicine, Department of Crop and Animal Sciences, Humboldt-Universität zu Berlin, Berlin, Germany.
[Ti] Título:Analysis of the complete genomes of Acholeplasma brassicae, A. palmae and A. laidlawii and their comparison to the obligate parasites from 'Candidatus Phytoplasma'.
[So] Source:J Mol Microbiol Biotechnol;24(1):19-36, 2014.
[Is] ISSN:1660-2412
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Analysis of the completely determined genomes of the plant-derived Acholeplasma brassicae strain O502 and A. palmae strain J233 revealed that the circular chromosomes are 1,877,792 and 1,554,229 bp in size, have a G + C content of 36 and 29%, and encode 1,690 and 1,439 proteins, respectively. Comparative analysis of these sequences and previously published genomes of A. laidlawii strain PG-8, 'Candidatus Phytoplasma asteris' strains, 'Ca. P. australiense' and 'Ca. P. mali' show a limited shared basic genetic repertoire. The acholeplasma genomes are characterized by a low number of rearrangements, duplication and integration events. Exceptions are the unusual duplication of rRNA operons in A. brassicae and an independently introduced second gene for a single-stranded binding protein in both genera. In contrast to phytoplasmas, the acholeplasma genomes differ by encoding the cell division protein FtsZ, a wide variety of ABC transporters, the F0F1 ATP synthase, the Rnf-complex, SecG of the Sec-dependent secretion system, a richly equipped repertoire for carbohydrate metabolism, fatty acid, isoprenoid and partial amino acid metabolism. Conserved metabolic proteins encoded in phytoplasma genomes such as the malate dehydrogenase SfcA, several transporters and proteins involved in host-interaction, and virulence-associated effectors were not predicted for the acholeplasmas.
[Mh] Termos MeSH primário: Acholeplasmataceae/genética
DNA Bacteriano/química
DNA Bacteriano/genética
Genoma Bacteriano
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Acholeplasmataceae/isolamento & purificação
Proteínas de Bactérias/genética
Composição de Bases
Ordem dos Genes
Dados de Sequência Molecular
Phytoplasma/genética
Sintenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140131
[Lr] Data última revisão:
140131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131026
[St] Status:MEDLINE
[do] DOI:10.1159/000354322


  3 / 32 MEDLINE  
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[PMID]:20960221
[Au] Autor:Zhang X; Zou Z; Gong P; Zhang J; Ziaf K; Li H; Xiao F; Ye Z
[Ad] Endereço:National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
[Ti] Título:Over-expression of microRNA169 confers enhanced drought tolerance to tomato.
[So] Source:Biotechnol Lett;33(2):403-9, 2011 Feb.
[Is] ISSN:1573-6776
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Plant miRNA regulates multiple developmental and physiological processes, including drought responses. We found that the accumulation of Sly-miR169 in tomato (Solanum lycopersicum) was induced by drought stress. Consequently, Sly-miR169 targets, namely, three nuclear factor Y subunit genes (SlNF-YA1/2/3) and one multidrug resistance-associated protein gene (SlMRP1), were significantly down-regulated by drought stress. Constitutive over-expression of a miR169 family member, Sly-miR169c, in tomato plant can efficiently down-regulate the transcripts of the target genes. Compared with non-transgenic plants, transgenic plants over-expressing Sly-miR169c displayed reduced stomatal opening, decreased transpiration rate, lowered leaf water loss, and enhanced drought tolerance. Our study is the first to provide evidence that the Sly-miR169c negatively regulates stomatal movement in tomato drought responses.
[Mh] Termos MeSH primário: Secas
Expressão Gênica
Lycopersicon esculentum/fisiologia
MicroRNAs/biossíntese
Estresse Fisiológico
[Mh] Termos MeSH secundário: Acholeplasmataceae
Lycopersicon esculentum/genética
Estômatos de Plantas/fisiologia
Plantas Geneticamente Modificadas/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1104
[Cu] Atualização por classe:110114
[Lr] Data última revisão:
110114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101021
[St] Status:MEDLINE
[do] DOI:10.1007/s10529-010-0436-0


  4 / 32 MEDLINE  
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[PMID]:19031034
[Au] Autor:Mayer CJ; Vilcinskas A; Gross J
[Ad] Endereço:Institute for Phytopathology and Applied Zoology, Justus-Liebig University, Giessen, 35392, Germany.
[Ti] Título:Pathogen-induced release of plant allomone manipulates vector insect behavior.
[So] Source:J Chem Ecol;34(12):1518-22, 2008 Dec.
[Is] ISSN:0098-0331
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infochemicals mediate communication within and between different trophic levels. In this study, we identified a new type of plant allomone induced by a plant pathogen and perceived by its vector insect Cacopsylla picta. This phloem-feeding psyllid is the main vector of Candidatus Phytoplasma mali, a cell wall-lacking bacterium that causes the so-called apple proliferation disease. In a previous study, we showed that newly emerged females of C. picta were attracted by the odor of phytoplasma-infected apple plants (Malus domestica), which release ss-caryophyllene in contrast to uninfected plants. Here, the attractiveness of this sesquiterpene for C. picta was confirmed in both olfactometer bioassays and field studies. Synthetic ss-caryophyllene was highly attractive to newly emerged adults of C. picta both when offered simultaneously with healthy apple odor and without. The psyllid's response was independent of its odor experience and infection status. These results confirm our previously established hypothesis that this phytoplasma manipulates the behavior of its vector insect by changing the odor blend of its host plant. Deployed in apple orchards, sticky traps baited with ss-caryophyllene dispensers caught both males and females of C. picta. Consequently, this new type of infochemical, i.e., a phytopathogen-induced plant allomone, represents a promising compound to develop innovative techniques for monitoring or maybe even mass trapping of C. picta.
[Mh] Termos MeSH primário: Acholeplasmataceae/fisiologia
Comportamento Animal/efeitos dos fármacos
Hemípteros/fisiologia
Insetos Vetores/fisiologia
Malus/metabolismo
Malus/microbiologia
Feromônios/metabolismo
[Mh] Termos MeSH secundário: Animais
Bioensaio
Feminino
Seguimentos
Hemípteros/crescimento & desenvolvimento
Interações Hospedeiro-Patógeno
Insetos Vetores/crescimento & desenvolvimento
Odorantes
Feromônios/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:0903
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:081126
[St] Status:MEDLINE
[do] DOI:10.1007/s10886-008-9564-6


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[PMID]:18523193
[Au] Autor:Cai H; Wei W; Davis RE; Chen H; Zhao Y
[Ad] Endereço:The Key Laboratory for Plant Pathology, Yunnan Agricultural University, Kunming 650201, PR China.
[Ti] Título:Genetic diversity among phytoplasmas infecting Opuntia species: virtual RFLP analysis identifies new subgroups in the peanut witches'-broom phytoplasma group.
[So] Source:Int J Syst Evol Microbiol;58(Pt 6):1448-57, 2008 Jun.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytoplasmas were detected in cactus (Opuntia species) plants exhibiting witches'-broom disease symptoms in Yunnan Province, south-western China. Comparative and phylogenetic analyses of 16S rRNA gene sequences indicated that an overwhelming majority of the cactus-infecting phytoplasmas under study belonged to the peanut witches'-broom phytoplasma group (16SrII). Genotyping through use of computer-simulated restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes revealed a remarkable genetic diversity among these cactus-infecting phytoplasma strains. Based on calculated coefficients of RFLP pattern similarities, seven new 16SrII subgroups were recognized, bringing the total of described group 16SrII subgroups to 12 worldwide. Geographical areas differed from one another in the extent of genetic diversity among cactus-infecting phytoplasma strains. The findings have implications for relationships between ecosystem distribution and the emergence of group 16SrII subgroup diversity.
[Mh] Termos MeSH primário: Acholeplasmataceae/classificação
Acholeplasmataceae/genética
Variação Genética
Opuntia/classificação
Opuntia/microbiologia
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: China
DNA Bacteriano/análise
DNA Ribossômico/análise
Ecossistema
Genótipo
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0808
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080605
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.65615-0


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[PMID]:18242720
[Au] Autor:Melito PL; Qiu X; Fernando LM; deVarennes SL; Beniac DR; Booth TF; Jones SM
[Ad] Endereço:Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg Manitoba, R3E 3R2 Canada. pasquale_melito@phac-aspc.gc.ca
[Ti] Título:The creation of stable cell lines expressing Ebola virus glycoproteins and the matrix protein VP40 and generating Ebola virus-like particles utilizing an ecdysone inducible mammalian expression system.
[So] Source:J Virol Methods;148(1-2):237-43, 2008 Mar.
[Is] ISSN:0166-0934
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ebola virus is a filovirus that causes hemorrhagic fever in humans and is associated with case fatality rates of up to 90%. The lack of therapeutic interventions in combination with the threat of weaponizing this organism has enhanced research investigations. The expression of key viral proteins and the production of virus-like particles in mammalian systems are often pursued for characterization and functional studies. Common practice is to express these proteins through transient transfection of mammalian cells. Unfortunately the transfection reagents are expensive and the process is time consuming and labour intensive. This work describes utilizing an ecdysone inducible mammalian expression system to create stable cell lines that express the Ebola virus transmembrane glycoprotein (GP), the soluble glycoprotein (sGP) and the matrix protein (VP40) individually as well as GP and VP40 simultaneously (for the production of virus like particles). These products were the same as those expressed by the transient system, by Western blot analysis and electron microscopy. The inducible system proved to be an improvement of the current technology by enhancing the cost effectiveness and simplifying the process.
[Mh] Termos MeSH primário: Ecdisona/farmacologia
Proteínas Estruturais Virais/biossíntese
Proteínas Estruturais Virais/genética
Virossomos/biossíntese
[Mh] Termos MeSH secundário: Acholeplasmataceae
Western Blotting
Linhagem Celular
Seres Humanos
Microscopia Eletrônica de Transmissão
Virossomos/química
Virossomos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Structural Proteins); 0 (Virosomes); 3604-87-3 (Ecdysone)
[Em] Mês de entrada:0805
[Cu] Atualização por classe:080222
[Lr] Data última revisão:
080222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080205
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2007.12.004


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[PMID]:18218949
[Au] Autor:Al-Saady NA; Khan AJ; Calari A; Al-Subhi AM; Bertaccini A
[Ad] Endereço:Department of Crop Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos University, PO Box 34, Al-Khod 123, Oman.
[Ti] Título:'Candidatus Phytoplasma omanense', associated with witches'-broom of Cassia italica (Mill.) Spreng. in Oman.
[So] Source:Int J Syst Evol Microbiol;58(Pt 2):461-6, 2008 Feb.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Samples from plants of Cassia italica exhibiting typical witches'-broom symptoms (Cassia witches'-broom; CWB) were examined for the presence of plant pathogenic phytoplasmas by PCR amplification using universal phytoplasma primers. All affected plants yielded positive results. RFLP analyses of rRNA gene products indicated that the phytoplasmas detected were different from those described previously. Phylogenetic analysis of 16S rRNA gene sequences confirmed that CWB represents a distinct lineage and shares a common ancestor with 'Candidatus Phytoplasma phoenicium'. Molecular comparison revealed that the 16S rRNA gene sequences of the four CWB strains (IM-1, IM-2, IM-3 and IM-4) identified in symptomatic C. italica samples were nearly identical (99.6-100 % similarity). The closest relatives were members of the pigeon pea witches'-broom phytoplasma ribosomal group (16SrIX; 95-97 % sequence similarity). On the basis of unique 16S rRNA gene sequences and biological properties, the phytoplasma associated with witches'-broom of C. italica in Oman represents a coherent but discrete novel phytoplasma, 'Candidatus Phytoplasma omanense', with GenBank/DDBJ/EMBL accession number EF666051 representing the reference strain.
[Mh] Termos MeSH primário: Acholeplasmataceae/classificação
Acholeplasmataceae/isolamento & purificação
Cassia/microbiologia
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Acholeplasmataceae/genética
Primers do DNA
DNA Bacteriano/análise
DNA Espaçador Ribossômico/análise
Dados de Sequência Molecular
Filogenia
Polimorfismo de Fragmento de Restrição
RNA Ribossômico 16S/genética
RNA Ribossômico 23S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S)
[Em] Mês de entrada:0804
[Cu] Atualização por classe:080125
[Lr] Data última revisão:
080125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080126
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.65425-0


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[PMID]:15280272
[Au] Autor:Marcone C; Schneider B; Seemüller E
[Ad] Endereço:Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Campus Macchia Romana, I-85100 Potenza, Italy. marcone@unibas.it
[Ti] Título:'Candidatus Phytoplasma cynodontis', the phytoplasma associated with Bermuda grass white leaf disease.
[So] Source:Int J Syst Evol Microbiol;54(Pt 4):1077-82, 2004 Jul.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bermuda grass white leaf (BGWL) is a destructive, phytoplasmal disease of Bermuda grass (Cynodon dactylon). The causal pathogen, the BGWL agent, differs from other phytoplasmas that cluster in the same major branch of the phytoplasma phylogenetic clade in <2.5% of 16S rDNA nucleotide positions, the threshold for assigning species rank to phytoplasmas under the provisional status 'Candidatus'. Thus, the objective of this work was to examine homogeneity of BGWL isolates and to determine whether there are, in addition to 16S rDNA, other markers that support delineation of the BGWL agent at the putative species level. Phylogenetic analyses revealed that the 16S rDNA sequences of BGWL strains were identical or nearly identical. Clear differences that support separation of the BGWL agent from related phytoplasmas were observed within the 16S-23S rDNA spacer sequence, by serological comparisons, in vector transmission and in host-range specificity. From these results, it can be concluded that the BGWL phytoplasma is a discrete taxon at the putative species level, for which the name 'Candidatus Phytoplasma cynodontis' is proposed. Strain BGWL-C1 was selected as the reference strain. Phytoplasmas that are associated with brachiaria white leaf, carpet grass white leaf and diseases of date palms showed 16S rDNA and/or 16S-23S rDNA spacer sequences that were identical or nearly identical to those of the BGWL phytoplasmas. However, the data available do not seem to be sufficient for a proper taxonomic assignment of these phytoplasmas.
[Mh] Termos MeSH primário: Cynodon/microbiologia
Phytoplasma/classificação
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Acholeplasmataceae
Impressões Digitais de DNA
DNA Bacteriano/química
DNA Ribossômico/química
DNA Espaçador Ribossômico/genética
Genes de RNAr
Dados de Sequência Molecular
Filogenia
Phytoplasma/genética
Phytoplasma/imunologia
Phytoplasma/isolamento & purificação
Polimorfismo de Fragmento de Restrição
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Homologia de Sequência
Sorotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0502
[Cu] Atualização por classe:040728
[Lr] Data última revisão:
040728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040729
[St] Status:MEDLINE


  9 / 32 MEDLINE  
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[PMID]:15023941
[Au] Autor:Lee IM; Martini M; Marcone C; Zhu SF
[Ad] Endereço:USDA-ARS Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA. leeim@ba.ars.usda.gov
[Ti] Título:Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of 'Candidatus Phytoplasma ulmi' for the phytoplasma associated with elm yellows.
[So] Source:Int J Syst Evol Microbiol;54(Pt 2):337-47, 2004 Mar.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Elm yellows group (16SrV) phytoplasmas, which are associated with devastating diseases in elm, grapevine, blackberry, cherry, peach and several other plant species in America, Europe and Asia, represent one of the most diverse phytoplasma clusters. On the basis of phylogenetic analysis of 16S rDNA sequences, elm yellows group phytoplasmas form a discrete subclade within the phytoplasma clade. Three phylogenetic parameters, namely 16S rRNA, ribosomal protein and secY genes, have been evaluated for their usefulness in differentiating elm yellows group phytoplasmas. RFLP analysis of 16S rRNA sequences differentiated the elm yellows group phytoplasmas into five subgroups. Twelve RFLP subgroups were differentiated on the basis of ribosomal protein and 13 were differentiated using secY gene sequences. Phylogenetic analysis of the ribosomal protein genes and secY gene alone or in combination indicated that the subgroups constitute 12 genetically distinct lineages, each of which appears to have evolved under different ecological constraints such as specific vector or plant hosts. On the basis of unique DNA and biological properties, it is proposed that the elm yellows phytoplasma EY1(T) represents a novel taxon, 'Candidatus Phytoplasma ulmi'.
[Mh] Termos MeSH primário: Acholeplasmataceae/classificação
Ulmus/microbiologia
[Mh] Termos MeSH secundário: Acholeplasmataceae/isolamento & purificação
Acholeplasmataceae/patogenicidade
Sequência de Bases
Primers do DNA
DNA Bacteriano/genética
DNA Ribossômico/genética
Dados de Sequência Molecular
Filogenia
Doenças das Plantas/microbiologia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0407
[Cu] Atualização por classe:040316
[Lr] Data última revisão:
040316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040317
[St] Status:MEDLINE


  10 / 32 MEDLINE  
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[PMID]:14521232
[Au] Autor:Davis RE; Jomantiene R; Kalvelyte A; Dally EL
[Ad] Endereço:Molecular Plant Pathology Laboratory, Plant Sciences Institute, Agricultural Research Service-USDA, Beltsville, MD 20705, USA. davisr@ba.ars.usda.gov
[Ti] Título:Differential amplification of sequence heterogeneous ribosomal RNA genes and classification of the 'Fragaria multicipita' phytoplasma.
[So] Source:Microbiol Res;158(3):229-36, 2003.
[Is] ISSN:0944-5013
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Ribosomal (r) RNA interoperon sequence heterogeneity in the 'Fragaria multicipita' phytoplasma, a member of group 16SrVI, was initially observed in RFLP patterns of rDNA amplified in the polymerase chain reaction (PCR), and was confirmed through sequence analysis of cloned rDNA. Sequences from operons rrnA and rrnB were amplified in PCR primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a phytoplasma into two different subgroups within a 16S rRNA group, if the phytoplasma's 16S rRNA gene sequences are independently characterized. It is suggested that the rRNA operon containing species-specific signature sequence(s) should be specified, and where possible sequences from both 16S rRNA genes should be included, in descriptions of new 'Candidatus Phytoplasma species'.
[Mh] Termos MeSH primário: Acholeplasmataceae/classificação
Acholeplasmataceae/genética
DNA Ribossômico
Polimorfismo de Fragmento de Restrição
Óperon de RNAr
[Mh] Termos MeSH secundário: Acholeplasmataceae/patogenicidade
DNA Bacteriano/genética
DNA Ribossômico/química
Fragaria/microbiologia
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Mapeamento por Restrição
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0311
[Cu] Atualização por classe:031002
[Lr] Data última revisão:
031002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:031003
[St] Status:MEDLINE



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