Base de dados : MEDLINE
Pesquisa : B03.440.860.300.750.625 [Categoria DeCS]
Referências encontradas : 544 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 55 ir para página                         

  1 / 544 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28889064
[Au] Autor:Hou L; Gu W; Zhu H; Yao W; Wang W; Meng Q
[Ad] Endereço:Jiangsu Key Laboratory for Microbes & Functional Genomics and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
[Ti] Título:Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.
[So] Source:Mol Immunol;91:75-85, 2017 11.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spiroplasma eriocheiris is a novel pathogen similar to the Spiroplasma mirum and also had an ability to infect the newborn mice and caused cataract. Our study was designed to study how S. eriocheiris infects mouse 3T6-Swiss albino cells and to elucidate the cellular molecular pathogenesis of Spiroplasma. FCM analysis and MTT analysis clearly shown that S. eriocheiris could induce 3T6 cell apoptosis and cause cell viability decreased seriously. Immunofluorescence experiments and TEM analysis shown that S. eriocheiris can invade 3T6 cells and form typical inclusion bodies and exhibit vacuolization in vitro. S. eriocheiris-oxytetracycline protection assay show that the infective bacteria already were detected at 1h post infection, and sharply increased at 12h after the bacteria infection. To further study the infection mechanism of S. eriocheiris, global mRNA and microRNA (miRNA) expression profiling were analyzed after the cells infected with the bacteria. A total of 619 non-redundant annotated transcripts (183 up-regulated and 436 down-regulated) and 22 miRNAs (8 up-regulated and 14 down-regulated) were differential expression after 6h S. eriocheiris infection compared to control group. Integrated analysis shown that homologous genes from differential expression miRNA targets and the differential expression genes of the mRNA microarray were major focused on two important pathways focal adhesion and MAPK signaling pathway. To validate the results of microarray, eight focal adhesion (ß-Catenin, Parvin, Grb2 and ERK) and MAPK signaling pathway (FGFR, Grb2, ERK, MKK3, p38 and JNK) genes and the housekeeping gene GAPDH were assayed by qPCR and Western blot to confirm the results. Eight miRNAs (miR-143-3p, miR-214-5p, miR-322-3p, miR-328-5p, miR-351-5p, miR-466h-5p, miR-503-5p and miR-30c-1-3p) and the housekeeping gene U6 miRNA were assayed by qPCR to confirm the results of microarray. All the results help us better understand the infection mechanism of S. eriocheiris.
[Mh] Termos MeSH primário: Apoptose/imunologia
Regulação da Expressão Gênica/imunologia
Infecções por Bactérias Gram-Negativas/imunologia
Spiroplasma/imunologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Infecções por Bactérias Gram-Negativas/genética
Infecções por Bactérias Gram-Negativas/patologia
Camundongos
MicroRNAs/genética
MicroRNAs/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170911
[St] Status:MEDLINE


  2 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28792419
[Au] Autor:Ning MX; Xiu YJ; Bi JX; Liu YH; Hou LB; Ding ZF; Gu W; Wang W; Meng QG
[Ad] Endereço:Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China.
[Ti] Título:Interaction of heat shock protein 60 (HSP60) with microRNA in Chinese mitten crab during Spiroplasma eriocheiris infection.
[So] Source:Dis Aquat Organ;125(3):207-215, 2017 08 09.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Heat shock protein 60 from the Chinese mitten crab Eriocheir sinensis (EsHSP60) was previously identified in relation to Spiroplasma eriocheiris infection by isobaric tags for relative and absolute quantitation labelling followed by liquid chromatography-tandem mass spectrometry. In the present study, to validate the immune function of this protein, the cDNA of the EsHSP60 gene was cloned. Various crab tissues were assessed using real-time PCR, which showed that EsHSP60 transcription occurred in all tissues examined. The expression profiles of EsHSP60 in haemolymph at transcription and protein levels when infected with S. eriocheiris were investigated by real-time PCR and Western blot analysis, respectively. A significant increase of EsHSP60 transcription and protein expression appeared post-injection in response to S. eriocheiris infection when compared to the control group. The double-luciferase reporter gene assay showed that the microRNA PC-533-3p interacted with the 3'-untranslated region of EsHSP60 and inhibited the translation of EsHSP60. The expression profiles of PC-533-3p during S. eriocheiris infection were also investigated by real-time PCR. However, the change tendency of PC-533-3p was opposite to that of the EsHSP60 after S. eriocheiris challenge. These data indicate that the EsHSP60 proteins may play an important role in mediating the immune responses of E. sinensis to an S. eriocheiris challenge.
[Mh] Termos MeSH primário: Braquiúros/microbiologia
Chaperonina 60/metabolismo
Regulação da Expressão Gênica/fisiologia
MicroRNAs/metabolismo
Spiroplasma/fisiologia
[Mh] Termos MeSH secundário: Animais
Braquiúros/genética
Braquiúros/metabolismo
Chaperonina 60/genética
Brânquias/metabolismo
Hemócitos/metabolismo
Hemolinfa
Hepatopâncreas/metabolismo
Interações Hospedeiro-Patógeno
Intestinos/metabolismo
MicroRNAs/genética
Músculos/metabolismo
Miocárdio/metabolismo
Neurônios/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chaperonin 60); 0 (MicroRNAs); 0 (RNA, Messenger)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.3354/dao03144


  3 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28609446
[Au] Autor:Murray RL; Herridge EJ; Ness RW; Bussière LF
[Ad] Endereço:Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada.
[Ti] Título:Are sex ratio distorting endosymbionts responsible for mating system variation among dance flies (Diptera: Empidinae)?
[So] Source:PLoS One;12(6):e0178364, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maternally inherited bacterial endosymbionts are common in many arthropod species. Some endosymbionts cause female-biased sex ratio distortion in their hosts that can result in profound changes to a host's mating behaviour and reproductive biology. Dance flies (Diptera: Empidinae) are well known for their unusual reproductive biology, including species with female-specific ornamentation and female-biased lek-like swarming behaviour. The cause of the repeated evolution of female ornaments in these flies remains unknown, but is probably associated with female-biased sex ratios in individual species. In this study we assessed whether dance flies harbour sex ratio distorting endosymbionts that might have driven these mating system evolutionary changes. We measured the incidence and prevalence of infection by three endosymbionts that are known to cause female-biased sex ratios in other insect hosts (Wolbachia, Rickettsia and Spiroplasma) across 20 species of dance flies. We found evidence of widespread infection by all three symbionts and variation in sex-specific prevalence across the taxa sampled. However, there was no relationship between infection prevalence and adult sex ratio measures and no evidence that female ornaments are associated with high prevalences of sex-biased symbiont infections. We conclude that the current distribution of endosymbiont infections is unlikely to explain the diversity in mating systems among dance fly species.
[Mh] Termos MeSH primário: Dípteros/microbiologia
Rickettsia/fisiologia
Spiroplasma/fisiologia
Simbiose
Wolbachia/fisiologia
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Dípteros/classificação
Feminino
Interações Hospedeiro-Patógeno
Modelos Lineares
Masculino
Reprodução
Razão de Masculinidade
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178364


  4 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28363870
[Au] Autor:Terahara N; Tulum I; Miyata M
[Ad] Endereço:Department of Biology, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. Electronic address: natsuholy@gmail.com.
[Ti] Título:Transformation of crustacean pathogenic bacterium Spiroplasma eriocheiris and expression of yellow fluorescent protein.
[So] Source:Biochem Biophys Res Commun;487(3):488-493, 2017 Jun 03.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Spiroplasma eriocheiris, the cause of crab trembling disease, is a wall-less bacterium, related to Mycoplasmas, measuring 2.0-10.0 µm long. It features a helical cell shape and a unique swimming mechanism that does not use flagella; instead, it moves by switching the cell helicity at a kink traveling from the front to the tail. S. eriocheiris seems to use a novel chemotactic system that is based on the frequency of reversal swimming behaviors rather than the conventional two-component system, which is generally essential for bacterial chemotaxis. To identify the genes involved in these novel mechanisms, we developed a transformation system by using oriC plasmid harboring the tetracycline resistant gene, tetM, which is under the control of a strong promoter for an abundant protein, elongation factor-Tu. The transformation efficiency achieved was 1.6 × 10 colony forming unit (CFU) for 1 µg DNA, enabling the expression of the enhanced yellow fluorescent protein (EYFP).
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Regulação Bacteriana da Expressão Gênica
Proteínas Luminescentes/genética
Spiroplasma/genética
Transformação Bacteriana
[Mh] Termos MeSH secundário: Microscopia de Fluorescência
Plasmídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Luminescent Proteins); 0 (yellow fluorescent protein, Bacteria)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170402
[St] Status:MEDLINE


  5 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28303252
[Au] Autor:Lan X; Zhang J; Zong Z; Ma Q; Wang Y
[Ad] Endereço:State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Evaluation of the Biocontrol Potential of QLP12 against in Eggplant.
[So] Source:Biomed Res Int;2017:4101357, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A fungus with broad spectrum antifungal activity was isolated from the soil in Qinling Mountain, Shaanxi Province, in China. The fungus was identified as based on ITS rDNA gene analysis. The strain, coded as QLP12, showed high inhibition activity on fungal mycelium growth , especially to , , and , and its potential for biocontrol efficacy of eggplant. wilt disease caused by among 10 fungal species tested was explored. In greenhouse experiments, QLP12 showed an excellent growth-promoting effect on eggplant seed germination (76.7%), bud growth (79.4%), chlorophyll content (47.83%), root activity (182.02%), and so on. QLP12 can colonize the eggplant interior and also develop in rhizosphere soil. In greenhouse, the incidence of wilt decreased by 83.82% with pretreated QLP12 fermentation broth in the soil. In the field, QLP12 showed prominent biocontrol effects on wilt by reducing the disease index over the whole growth period, a decline of 40.1%. This study showed that the strain QLP12 is not only an effective biocontrol agent for controlling wilt of eggplant, but also a plant growth-promoting fungus that deserves to be further developed.
[Mh] Termos MeSH primário: Controle Biológico de Vetores
Solanum melongena/microbiologia
Spiroplasma/crescimento & desenvolvimento
Verticillium/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: China
Germinação/fisiologia
Doenças das Plantas/microbiologia
Raízes de Plantas/microbiologia
Microbiologia do Solo
Solanum melongena/crescimento & desenvolvimento
Spiroplasma/patogenicidade
Verticillium/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1155/2017/4101357


  6 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28184355
[Au] Autor:Hou L; Liu Y; Gao Q; Xu X; Ning M; Bi J; Liu H; Liu M; Gu W; Wang W; Meng Q
[Ad] Endereço:Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University Nanjing, China.
[Ti] Título: Adhesin-Like Protein (ALP) Interacts with Epidermal Growth Factor (EGF) Domain Proteins to Facilitate Infection.
[So] Source:Front Cell Infect Microbiol;7:13, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is a novel pathogen found in recent years, causing the tremor disease (TD) of Chinese mitten crab . Like infects the newborn mouse (adult mice are not infected) and can cause cataract. Adhesion-related protein is an important protein involved in the interaction between pathogen and host. In this study, the Adhesin-like Protein (ALP) of was detected on its outer membrane by using immune electron microscopy, and was found to be involved in the bacterium's infection of mouse embryo fibroblasts (3T6-Swiss albino). Yeast two-hybrid analysis demonstrated that ALP interacts with a diverse group of mouse proteins. The interactions between recombinant partial fibulin7 (FBLN7; including two epidermal growth factor [EGF] domains) and ALP were confirmed by Far-western blotting and colocalization. We synthetized the domains of FBLN7 [EGF domain: amino acids 136-172 and complement control protein (CCP) domain: 81-134 amino acids], and demonstrated that only EGF domain of FBLN7 can interact with ALP. Because the EGF domain has high degree of similarity to EGF, it can activate the downstream EGFR signaling pathway, in key site amino acids. The EGFR pathway in 3T6 cells was restrained after rALP stimulation resulting from competitive binding of ALP to EGF. The unborn mouse, newborn mouse, and the adult mouse with cataract have a small amount of expressed FBLN7; however, none was detected in the brain and very little expression was seen in the eye of normal adult mice. In short, ALP as a surface protein, is critical for infection and further supports the role of ALP in infection by competitive effection of the EGF/EGFR axis of the target cells.
[Mh] Termos MeSH primário: Adesinas Bacterianas/metabolismo
Proteínas da Membrana Bacteriana Externa/metabolismo
Proteínas de Ligação ao Cálcio/metabolismo
Endocitose
Interações Hospedeiro-Patógeno
Spiroplasma/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Fator de Crescimento Epidérmico/metabolismo
Fibroblastos/microbiologia
Camundongos
Microscopia Imunoeletrônica
Ligação Proteica
Mapeamento de Interação de Proteínas
Receptor do Fator de Crescimento Epidérmico/metabolismo
Transdução de Sinais
Spiroplasma/química
Técnicas do Sistema de Duplo-Híbrido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adhesins, Bacterial); 0 (Bacterial Outer Membrane Proteins); 0 (Calcium-Binding Proteins); 0 (fibulin); 62229-50-9 (Epidermal Growth Factor); EC 2.7.10.1 (EGFR protein, mouse); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00013


  7 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27926815
[Au] Autor:Zhu H; Liu P; Du J; Wang J; Jing Y; Zhang J; Gu W; Wang W; Meng Q
[Ad] Endereço:2​Laboratory of Animal Improvement and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China 1​Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences,
[Ti] Título:Identification of lysophospholipase protein from Spiroplasma eriocheiris and verification of its function.
[So] Source:Microbiology;163(2):175-184, 2017 Feb.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spiroplasma eriocheiris is known to cause tremor disease in the Chinese mitten crab Eriocheir sinensis; however, the molecular characterization of this pathogen is still unclear. S. eriocheiris has the ability to invade and survive within mouse 3T6 cells. The invasion process may require causing damage to the host cell membrane by chemical, physical or enzymatic means. In this study, we systematically characterized a novel lysophospholipase (lysoPL) of S. eriocheiris TDA-040725-5T. The gene that encodes lysoPL in S. eriocheiris (SE-LysoPL) was cloned, sequenced and expressed in Escherichia coli BL21 (DE3). Enzymatic assays revealed that the purified recombinant SE-LysoPL hydrolysed long-chain acyl esterases at pH 7 and 30 °C. SE-LysoPL was detected in the membrane and cytoplasmic protein fractions using the SE-LysoPL antibody in Western blot. The virulence ability of S. eriocheiris was effectively reduced at the early stage of infection (m.o.i.=100) by the SE-LysoPL antibody neutralization test. To the best of our knowledge, this is the first study to identify and characterize a gene from S. eriocheiris encoding a protein exhibiting lysoPL and esterase activities. Our findings indicate that SE-LysoPL plays important roles in the pathogenicity of S. eriocheiris.
[Mh] Termos MeSH primário: Anticorpos Neutralizantes/imunologia
Braquiúros/microbiologia
Lisofosfolipase/genética
Lisofosfolipase/imunologia
Spiroplasma/patogenicidade
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Linhagem Celular
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Camundongos
Alinhamento de Sequência
Spiroplasma/enzimologia
Spiroplasma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); EC 3.1.1.5 (Lysophospholipase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161208
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.000407


  8 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26773849
[Au] Autor:Sepúlveda DA; Zepeda-Paulo F; Ramírez CC; Lavandero B; Figueroa CC
[Ad] Endereço:Facultad de Ciencias Agrarias, Universidad de Talca, 2 Norte 685, Talca, Chile.
[Ti] Título:Diversity, frequency, and geographic distribution of facultative bacterial endosymbionts in introduced aphid pests.
[So] Source:Insect Sci;24(3):511-521, 2017 Jun.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Facultative bacterial endosymbionts in insects have been under intense study during the last years. Endosymbionts can modify the insect's phenotype, conferring adaptive advantages under environmental stress. This seems particularly relevant for a group of worldwide agricultural aphid pests, because endosymbionts modify key fitness-related traits, including host plant use, protection against natural enemies and heat tolerance. Aimed to understand the role of facultative endosymbionts on the success of introduced aphid pests, the distribution and abundance of 5 facultative endosymbionts (Hamiltonella defensa, Regiella insecticola, Serratia symbiotica, Rickettsia and Spiroplasma) were studied and compared in 4 cereal aphids (Sitobion avenae, Diuraphis noxia, Metopolophium dirhodum and Schizaphis graminium) and in the pea aphid Acyrthosiphon pisum complex from 2 agroclimatic zones in Chile. Overall, infections with facultative endosymbionts exhibited a highly variable and characteristic pattern depending on the aphid species/host race and geographic zone, which could explain the success of aphid pest populations after their introduction. While S. symbiotica and H. defensa were the most frequent endosymbionts carried by the A. pisum pea-race and A. pisum alfalfa-race aphids, respectively, the most frequent facultative endosymbiont carried by all cereal aphids was R. insecticola. Interestingly, a highly variable composition of endosymbionts carried by S. avenae was also observed between agroclimatic zones, suggesting that endosymbionts are responding differentially to abiotic variables (temperature and precipitations). In addition, our findings constitute the first report of bacterial endosymbionts in cereal aphid species not screened before, and also the first report of aphid endosymbionts in Chile.
[Mh] Termos MeSH primário: Afídeos/microbiologia
Enterobacteriaceae/isolamento & purificação
Simbiose
[Mh] Termos MeSH secundário: Animais
Rickettsia/isolamento & purificação
Spiroplasma/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160117
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12313


  9 / 544 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27875577
[Au] Autor:Heyworth ER; Ferrari J
[Ad] Endereço:Department of Biology, University of York, York, United Kingdom.
[Ti] Título:Heat Stress Affects Facultative Symbiont-Mediated Protection from a Parasitoid Wasp.
[So] Source:PLoS One;11(11):e0167180, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many insects carry facultative bacterial symbionts, which provide benefits including resistance to natural enemies and abiotic stresses. Little is known about how these beneficial phenotypes are affected when biotic or abiotic threats occur simultaneously. The pea aphid (Acyrthosiphon pisum) can host several well-characterized symbiont species. The symbiont known as X-type can protect against both parasitoid wasps and heat stress. Here, we used three pea aphid genotypes that were naturally infected with X-type and the symbiont Spiroplasma sp. We compared aphids coinfected with these two symbionts with those cured from X-type and infected with only Spiroplasma to investigate the ability of X-type to confer benefits to the host when two threats are experienced simultaneously. Our aim is to explore how robust symbiont protection may be outside a benign laboratory environment. Aphids were subjected to heat shock either before or after attack by parasitoid wasps. Under a benign temperature regime, the aphids carrying X-type tended to be better protected from the parasitoid than those cured. When the aphids experienced a heat shock before being parasitized aphids carrying X-type were more susceptible than those cured. Regardless of infection with the symbiont, the aphids benefitted from being heat shocked after parasitization. The results demonstrate how resistance to parasitoid wasps can be strongly environment-dependent and that a beneficial phenotype conferred by a symbiont under controlled conditions in the laboratory does not necessarily equate to a consistently useful effect in natural populations.
[Mh] Termos MeSH primário: Afídeos/microbiologia
Resposta ao Choque Térmico/fisiologia
Spiroplasma/fisiologia
Simbiose/fisiologia
Vespas/microbiologia
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167180


  10 / 544 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27760404
[Au] Autor:Desfougères Y; Poitou JM; Wróblewski H; Béven L
[Ad] Endereço:UMR Université-CNRS 6026, Université de Rennes 1, Campus de Beaulieu, Bâtiment 13, 35042 Rennes Cedex, France. Electronic address: y.desfougeres@ucl.ac.uk.
[Ti] Título:An improved non-denaturing method for the purification of spiralin, the main membrane lipoprotein of the pathogenic bacteria Spiroplasma melliferum.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1036-1037:149-156, 2016 Nov 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spiralin is the most abundant protein of several species of spiroplasmas, helical, motile bacteria pathogenic for arthropods and plants. This amphiphilic protein is anchored to the outer face of the plasma membrane by a lipoylated N-terminal cysteine. Although spiroplasma pathogenicity in mammals is controversial, it was shown that spiralin is highly immunogenic and endowed with immunomodulatory activity. In this paper, we describe a high performance method for the purification of Spiroplasma melliferum spiralin under non-denaturing conditions. The protein was selectively extracted with 3-[(3-cholamidopropyl) dimethylammonio]-1-propyl sulfonate (CHAPS) from the membrane pre-treated with sodium dodecyl-N-sarcosinate (Sarkosyl), and purified to homogeneity by cation-exchange HPLC with an overall yield of ∼60%. Detergent-depleted, water-soluble micelles of spiralin displaying a mean diameter of 170Å, as evidenced by transmission electron microscopy, were obtained by dialysis detergent removal. Circular dichroism spectroscopy and cross immunoprecipitation assay of the purified spiralin strongly suggested that this purification method could retain the structural characteristics of the native spiralin. The strategy developed to purify spiralin (two successive selective extractions of membrane proteins with mild detergents followed by ion-exchange chromatography) should prove useful for the purification of membrane lipoproteins of other bacteria of the class Mollicutes including different pathogens for humans, animals and plants.
[Mh] Termos MeSH primário: Proteínas da Membrana Bacteriana Externa/isolamento & purificação
Cromatografia por Troca Iônica/métodos
Spiroplasma/química
[Mh] Termos MeSH secundário: Proteínas da Membrana Bacteriana Externa/química
Ácidos Cólicos/química
Cromatografia em Gel/métodos
Cromatografia Líquida de Alta Pressão/métodos
Dicroísmo Circular
Detergentes/química
Conformação Proteica
Desnaturação Proteica
Sarcosina/análogos & derivados
Sarcosina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Cholic Acids); 0 (Detergents); 0 (spiralin); 632GS99618 (sarkosyl); QBP25342AG (3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate); Z711V88R5F (Sarcosine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161027
[St] Status:MEDLINE



página 1 de 55 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde