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  1 / 43 MEDLINE  
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[PMID]:29311457
[Au] Autor:Kenzaka T; Kataoka K; Fujimitsu T; Tani K
[Ad] Endereço:Environmental Science and Microbiology, Faculty of Pharmacy, Osaka Ohtani University.
[Ti] Título:[Intestinal Microbiota in Migrating Barn Swallows around Osaka].
[So] Source:Yakugaku Zasshi;138(1):117-122, 2018.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Migratory birds are considered as vectors of infectious diseases, owing to their potential for transmitting pathogens over large distances. The populations of barn swallow (Hirundo rustica) migrate from Southeast Asia to the Japanese mainland during spring and migrate back to Southeast Asia during autumn. This migratory population is estimated to comprise approximately hundreds to thousands of individuals per year. However, to date, not much is known about the gastrointestinal microbiota of the barn swallow. In this study, we characterized the fecal bacterial community in barn swallow. Using 16S rRNA gene metagenomic sequencing analysis, we examined the presence and composition of potentially pathogenic bacteria in the fecal samples, which were collected during spring season from Osaka. The number (±S.D.) of total bacteria was approximately 2.1(±3.4)×10 per gram of feces. In most samples, the bacterial community composition was dominated by families, such as Enterobacteriaceae, Pseudomonadaceae, Mycoplasmataceae, Enterococcaceae, Streptococcaceae, and Alcaligenaceae. However, no relationship was found between the bacterial community composition and geographical area in the fecal samples. Potentially pathogenic bacteria were detected at the rate of >0.1%, which included Pseudomonas spp., Escherichia/Shigella spp., Enterobacter spp., Yersinia spp., Mycoplasma spp., Enterococcus spp., Achromobacter spp., and Serratia spp. Our results suggested that barn swallow is instrumental in the transmission of these genera over large distances.
[Mh] Termos MeSH primário: Migração Animal/fisiologia
Vetores de Doenças
Intestinos/microbiologia
Microbiota
Andorinhas/microbiologia
[Mh] Termos MeSH secundário: Alcaligenaceae/isolamento & purificação
Alcaligenaceae/patogenicidade
Animais
Ásia Sudeste
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/patogenicidade
Enterococcaceae/isolamento & purificação
Enterococcaceae/patogenicidade
Fezes/microbiologia
Japão
Mycoplasmataceae/isolamento & purificação
Mycoplasmataceae/patogenicidade
Pseudomonadaceae/isolamento & purificação
Pseudomonadaceae/patogenicidade
Streptococcaceae/isolamento & purificação
Streptococcaceae/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.17-00148


  2 / 43 MEDLINE  
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[PMID]:28381682
[Au] Autor:Fu Y; Shi T; Xu L; Wei W; Lu F; Zhang X; Yuan X; Li J; Lv J; Fang W
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.
[Ti] Título:Identification of a novel Hemoplasma species from pigs in Zhejiang province, China.
[So] Source:J Vet Med Sci;79(5):864-870, 2017 May 18.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Hemoplasmas belong to Mycoplasmataceae (Mollicutes: Mycoplasmatales) and are able to infect a broad range of mammalian species. We investigated prevalence of hemotropic mycoplasma species in pig farms in the region of Zhejiang by a PCR scheme using universal primers targeting 16S rRNA and RNase P RNA gene (rnpB). Representative positive samples from different farms were selected for sequencing of 16S rRNA and the 219bp rnpB gene fragments for phylogenetic analysis. Sequencing analysis of PCR products from first samples identified a novel hemoplasma species present in several pig farms in the region with highest nucleotide identity of 92% to Candidatus Mycoplasma turicensis. A duplex PCR assay was then designed for differential detection of the novel hemoplasma from Mycoplasma parvum/M. suis in field samples. Of 324 blood samples from clinically healthy pigs, 26.5% was positive for this novel hemoplasma species and 50% positive for M. suis/M. parvum, indicating that the novel hemotropic mycoplasma species were of considerably high prevalence in Zhejiang province, China.
[Mh] Termos MeSH primário: Mycoplasmataceae/isolamento & purificação
Infecções por Mycoplasmatales/veterinária
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
China
Mycoplasmataceae/classificação
Infecções por Mycoplasmatales/microbiologia
Filogenia
Reação em Cadeia da Polimerase/veterinária
RNA Ribossômico 16S
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0545


  3 / 43 MEDLINE  
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[PMID]:26747447
[Au] Autor:Tatarinova TV; Lysnyansky I; Nikolsky YV; Bolshoy A
[Ad] Endereço:Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, 90027, CA, USA. tatiana.tatarinova@usc.edu.
[Ti] Título:The mysterious orphans of Mycoplasmataceae.
[So] Source:Biol Direct;11(1):2, 2016 Jan 08.
[Is] ISSN:1745-6150
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The length of a protein sequence is largely determined by its function. In certain species, it may be also affected by additional factors, such as growth temperature or acidity. In 2002, it was shown that in the bacterium Escherichia coli and in the archaeon Archaeoglobus fulgidus, protein sequences with no homologs were, on average, shorter than those with homologs (BMC Evol Biol 2:20, 2002). It is now generally accepted that in bacterial and archaeal genomes the distributions of protein length are different between sequences with and without homologs. In this study, we examine this postulate by conducting a comprehensive analysis of all annotated prokaryotic genomes and by focusing on certain exceptions. RESULTS: We compared the distribution of lengths of "having homologs proteins" (HHPs) and "non-having homologs proteins" (orphans or ORFans) in all currently completely sequenced and COG-annotated prokaryotic genomes. As expected, the HHPs and ORFans have strikingly different length distributions in almost all genomes. As previously established, the HHPs, indeed are, on average, longer than the ORFans, and the length distributions for the ORFans have a relatively narrow peak, in contrast to the HHPs, whose lengths spread over a wider range of values. However, about thirty genomes do not obey these rules. Practically all genomes of Mycoplasma and Ureaplasma have atypical ORFans distributions, with the mean lengths of ORFan larger than the mean lengths of HHPs. These genera constitute over 80 % of atypical genomes. CONCLUSIONS: We confirmed on a ubiquitous set of genomes that the previous observation of HHPs and ORFans have different gene length distributions. We also showed that Mycoplasmataceae genomes have very distinctive distributions of ORFans lengths. We offer several possible biological explanations of this phenomenon, such as an adaptation to Mycoplasmataceae's ecological niche, specifically its "quiet" co-existence with host organisms, resulting in long ABC transporters.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Mycoplasmataceae/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Genoma Bacteriano/genética
Mycoplasmataceae/genética
Fases de Leitura Aberta/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160110
[St] Status:MEDLINE
[do] DOI:10.1186/s13062-015-0104-3


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[PMID]:25892209
[Au] Autor:Takanashi M; Ito S; Kaneto H; Tanahashi Y; Kitanohara M; Yanagihara A; Nakazima H; Yasuda M
[Ad] Endereço:Infectious Disease Testing Department, LSI Medience Corporation, Tokyo, Japan. Electronic address: takanashi.masaki@ms.medience.co.jp.
[Ti] Título:Development and clinical application of an InvaderPlus® assay for the detection of genital mycoplasmas.
[So] Source:J Infect Chemother;21(7):516-9, 2015 Jul.
[Is] ISSN:1437-7780
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We developed a PCR-based assay involving Invader® technology for detection of the genital mycoplasmas of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. We compared its performance with that of a PCR-microtiter plate hybridization assay, which we developed previously, in detecting genital mycoplasmas in first-voided urine (FVU) specimens from men with non-gonococcal urethritis. The tests targeting each of the genital mycoplasmas were specific for the respective species and could detect as few as 10 copies of the plasmids containing the target genes of each of the genital mycoplasmas per reaction. The assay using the InvaderPlus® method (InvaderPlus® assay) showed very similar performance to that of the PCR-microtiter plate hybridization assay for detecting the genital mycoplasmas in the FVU specimens. In addition, the PCR and endonuclease reaction in the InvaderPlus® assay were carried out simultaneously in one procedure, thus simplifying the assay, leading to time- and labor-savings and a decrease in the risk of specimen contamination. The InvaderPlus® assay could be useful in diagnosing genitourinary tract infections caused by the genital mycoplasmas.
[Mh] Termos MeSH primário: Doenças Urogenitais Masculinas/microbiologia
Tipagem Molecular/métodos
Mycoplasmataceae/genética
Infecções por Mycoplasmatales/microbiologia
[Mh] Termos MeSH secundário: DNA Bacteriano/análise
DNA Bacteriano/genética
Seres Humanos
Masculino
Doenças Urogenitais Masculinas/diagnóstico
Infecções por Mycoplasmatales/diagnóstico
Reação em Cadeia da Polimerase/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150615
[Lr] Data última revisão:
150615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE


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[PMID]:24482531
[Au] Autor:Leclercq S; Dittmer J; Bouchon D; Cordaux R
[Ad] Endereço:Université de Poitiers, UMR CNRS 7267 Ecologie et Biologie des Interactions, Equipe Ecologie Evolution Symbiose, Poitiers, France.
[Ti] Título:Phylogenomics of "Candidatus Hepatoplasma crinochetorum," a lineage of mollicutes associated with noninsect arthropods.
[So] Source:Genome Biol Evol;6(2):407-15, 2014 Feb.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacterial gut communities of arthropods are highly diverse and tightly related to host feeding habits. However, our understanding of the origin and role of the symbionts is often hindered by the lack of genetic information. "Candidatus Hepatoplasma crinochetorum" is a Mollicutes symbiont found in the midgut glands of terrestrial isopods. The only available nucleotide sequence for this symbiont is a partial 16S rRNA gene sequence. Here, we present the 657,101 bp assembled genome of Candidatus Hepatoplasma crinochetorum isolated from the terrestrial isopod Armadillidium vulgare. While previous 16S rRNA gene-based analyses have provided inconclusive results regarding the phylogenetic position of Candidatus Hepatoplasma crinochetorum within Mollicutes, we performed a phylogenomic analysis of 127 Mollicutes orthologous genes which confidently branches the species as a sister group to the Hominis group of Mycoplasma. Several genome properties of Candidatus Hepatoplasma crinochetorum are also highlighted compared with other Mollicutes genomes, including adjacent tryptophan tRNA genes, which further our understanding of the evolutionary dynamics of these genes in Mollicutes, and the presence of a probably inactivated CRISPR/Cas system, which constitutes a testimony of past interactions between Candidatus Hepatoplasma crinochetorum and mobile genetic elements, despite their current lack in this streamlined genome. Overall, the availability of the complete genome sequence of Candidatus Hepatoplasma crinochetorum paves the way for further investigation of its ecology and evolution.
[Mh] Termos MeSH primário: Genoma Bacteriano
Isópodes/microbiologia
Mycoplasmataceae/classificação
Mycoplasmataceae/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Evolução Molecular
Isópodes/fisiologia
Dados de Sequência Molecular
Mycoplasmataceae/isolamento & purificação
Mycoplasmataceae/fisiologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140201
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evu020


  6 / 43 MEDLINE  
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[PMID]:22930310
[Au] Autor:Wang B; Wu JR; Guo HJ; Yang HT; Ai J; Hui M; Chan CY
[Ad] Endereço:School of Public Health, Southeast University, Nanjing, Jiangsu 210009, People's Republic of China. wangbeilxb@seu.edu.cn
[Ti] Título:The prevalence of six species of Mycoplasmataceae in an HIV/AIDS population in Jiangsu Province, China.
[So] Source:Int J STD AIDS;23(8):e7-10, 2012 Aug.
[Is] ISSN:1758-1052
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study employed culture and polymerase chain reaction (PCR) to examine the prevalence of Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Mycoplasma fermentans, Mycoplasma penetrans and Mycoplasma pirum in 210 HIV/AIDS patients, 455 sexually transmitted infection (STI) clinic attendees and 245 healthy volunteers from first-void urine specimens for men and endocervical swabs for women. U. urealyticum and M. hominis were detected in 107 (51.0%) and 69 (32.9%) patients in the HIV/AIDS group. At least one of the other four organisms was detected in 34 (16.2%) HIV/AIDS patients, 29 (6.4%) STI clinic attendees and six (2.5%) healthy volunteers. This study showed that U. urealyticum, M. hominis and M. fermentans were significantly more prevalent in HIV/AIDS patients, as were other mycoplasmas. Our results suggest a possible role for co-infection.
[Mh] Termos MeSH primário: Infecções Oportunistas Relacionadas com a AIDS/epidemiologia
Mycoplasmataceae
Infecções por Mycoplasmatales/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Criança
Pré-Escolar
China/epidemiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Mycoplasma
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/microbiologia
Mycoplasma fermentans
Mycoplasma genitalium
Mycoplasma hominis
Mycoplasma penetrans
Infecções por Mycoplasmatales/microbiologia
Reação em Cadeia da Polimerase
Prevalência
Infecções por Ureaplasma/epidemiologia
Infecções por Ureaplasma/microbiologia
Ureaplasma urealyticum
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1211
[Cu] Atualização por classe:120829
[Lr] Data última revisão:
120829
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:120830
[St] Status:MEDLINE
[do] DOI:10.1258/ijsa.2009.009396


  7 / 43 MEDLINE  
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[PMID]:22816103
[Au] Autor:Lushnikova EL; Nepomnyashchikh LM; Abdullaev NA; Klepikova II; Molodykh OP; Neimark AI
[Ad] Endereço:Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. pathol@soramn.ru
[Ti] Título:Role of sexually-transmitted infections in the structural and functional reorganization of the prostate.
[So] Source:Bull Exp Biol Med;153(2):283-8, 2012 Jun.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng; rus
[Ab] Resumo:Intracellular reorganization of secretory epitheliocytes in the main, intermediate, and periurethral prostatic glands was studied in chronic prostatitis under conditions of sexually-transmitted infections. The destructive and autophagic processes in the secretory epitheliocytes were stimulated by persistence of microorganisms, Mycoplasmataceae (mainly mycoplasmas and ureaplasmas) and Chlamydia trachomatis, in the prostatic terminal compartments, epithelial layer, and epitheliocytes. Significant intracellular reorganization of smooth-muscle cells was found: focal destruction of ultrastructures (mainly in the perinuclear zone) and lythic changes in the myofilaments (focal and diffuse).
[Mh] Termos MeSH primário: Próstata/patologia
Prostatite/patologia
Doenças Sexualmente Transmissíveis/microbiologia
Doenças Sexualmente Transmissíveis/patologia
[Mh] Termos MeSH secundário: Adulto
Infecções por Chlamydia/microbiologia
Infecções por Chlamydia/patologia
Chlamydia trachomatis/isolamento & purificação
Seres Humanos
Masculino
Infecções por Mycoplasma/microbiologia
Infecções por Mycoplasma/patologia
Mycoplasmataceae/isolamento & purificação
Miócitos de Músculo Liso/microbiologia
Miócitos de Músculo Liso/ultraestrutura
Próstata/microbiologia
Prostatite/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1301
[Cu] Atualização por classe:120720
[Lr] Data última revisão:
120720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120721
[St] Status:MEDLINE


  8 / 43 MEDLINE  
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[PMID]:22729196
[Au] Autor:Costoya A; Morales F; Borda P; Vargas R; Fuhrer J; Salgado N; Cárdenas H; Velasquez L
[Ad] Endereço:Departament of Obstetrics and Gynecology, Medical School, Universidad de Santiago de Chile, Santiago, Chile.
[Ti] Título:Mycoplasmateceae species are not found in Fallopian tubes of women with tubo-peritoneal infertility.
[So] Source:Braz J Infect Dis;16(3):273-8, 2012 May-Jun.
[Is] ISSN:1678-4391
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The role of mycoplasmas on the development and sequelae of pelvic inflammatory disease remains controversial. The objective of the present study is to correlate directly the presence of Mycoplasmateceae through polimerase chain reaction (PCR) determinations in cervix and Fallopian tubes of infertile patients with tubo-peritoneal factor diagnosed through laparoscopy. METHODS: Thirty patients with tubo-peritoneal infertility and 30 normal fertile patients were included in the study; cervical samples and tubal flushings were obtained during laparoscopy. PCR determinations for the detection of genetic material of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealiticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis in cervix and tubal flushings were performed. RESULTS: No Mycoplasmataceae species as "only" microorganisms were found in tubal flushings of tubo-peritoneal infertility patients, whereas three (10%) fertile patients with normal tubes were positive for mycoplasma presence. This difference was not significant (p = 0.237). Among the 30 patients suffering from tubal infertility diagnosed through laparoscopy, Mycoplasmatecae species were not detected in the Fallopian tubes by PCR determinations, while in normal tubes from fertile patients these and other microorganisms could be found without distorting tubal anatomy. CONCLUSION: Mycoplasmateceae species were not detected in Fallopian tubes of women with tubo-peritoneal infertility.
[Mh] Termos MeSH primário: Doenças das Tubas Uterinas/microbiologia
Infertilidade Feminina/microbiologia
Infecções por Mycoplasma/microbiologia
Mycoplasmataceae/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Reação em Cadeia da Polimerase Multiplex
Infecções por Mycoplasma/diagnóstico
Mycoplasma genitalium/isolamento & purificação
Mycoplasma hominis/isolamento & purificação
Mycoplasmataceae/classificação
Estudos Prospectivos
Ureaplasma/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1211
[Cu] Atualização por classe:170225
[Lr] Data última revisão:
170225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120626
[St] Status:MEDLINE


  9 / 43 MEDLINE  
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[PMID]:22252268
[Au] Autor:Lee SJ; Park DC; Lee DS; Choe HS; Cho YH
[Ad] Endereço:Department of Urology, St. Vincent's Hospital, The Catholic University of Korea College of Medicine, Suwon, Korea.
[Ti] Título:Evaluation of Seeplex® STD6 ACE Detection kit for the diagnosis of six bacterial sexually transmitted infections.
[So] Source:J Infect Chemother;18(4):494-500, 2012 Aug.
[Is] ISSN:1437-7780
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Traditionally, the diagnosis of bacterial sexually transmitted infection (STI) has been dependent on the isolation of the causative pathogens by culturing endocervical or urethral swab specimens on selective media. While such procedures typically provide excellent diagnostic accuracy, they are often time-consuming and expensive. A multiplex polymerase chain reaction (PCR) assay, based on a semi-automated detection system, was evaluated for the detection of six STI causative organisms. The Seeplex(®) STD6 ACE (auto-capillary electrophoresis) Detection assay employed six pairs of dual priming oligonucleotide (DPO™) primers specifically targeted to unique genes of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis. A total of 739 specimens (304 cervical swabs and 435 urine samples) collected for 4 months were tested, and results were compared to those obtained with a combined monoplex PCR. The concordance between the multiplex PCR and monoplex PCR assay was 100% for both sensitivity and specificity. We also tested for the presence of two pathogenic bacteria (C. trachomatis and N. gonorrhoeae) and compared the results obtained with the multiplex PCR and BD ProbeTec duplex strand displacement amplification (SDA). The results of the multiplex PCR and duplex SDA were 99.7% concordant for C. trachomatis and 100% concordant for N. gonorrhoeae. The multiplex PCR assay using the Seeplex(®) STD6 ACE Detection kit proved to be a novel cost-effective and fast diagnostic tool with high sensitivity and specificity for the simultaneous detection of six STI pathogens.
[Mh] Termos MeSH primário: Reação em Cadeia da Polimerase/métodos
Kit de Reagentes para Diagnóstico
Doenças Sexualmente Transmissíveis/diagnóstico
Doenças Sexualmente Transmissíveis/microbiologia
[Mh] Termos MeSH secundário: Adulto
Colo do Útero/microbiologia
Colo do Útero/parasitologia
Chlamydia trachomatis/genética
Chlamydia trachomatis/isolamento & purificação
Primers do DNA/genética
Feminino
Seres Humanos
Masculino
Meia-Idade
Mycoplasmataceae/genética
Mycoplasmataceae/isolamento & purificação
Infecções por Mycoplasmatales/diagnóstico
Infecções por Mycoplasmatales/microbiologia
Neisseria gonorrhoeae/genética
Neisseria gonorrhoeae/isolamento & purificação
Sensibilidade e Especificidade
Doenças Sexualmente Transmissíveis/parasitologia
Doenças Sexualmente Transmissíveis/urina
Vaginite por Trichomonas/diagnóstico
Vaginite por Trichomonas/parasitologia
Trichomonas vaginalis/genética
Trichomonas vaginalis/isolamento & purificação
Urina/microbiologia
Urina/parasitologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1303
[Cu] Atualização por classe:120813
[Lr] Data última revisão:
120813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120119
[St] Status:MEDLINE
[do] DOI:10.1007/s10156-011-0362-7


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[PMID]:22115576
[Au] Autor:Volokhov DV; Simonyan V; Davidson MK; Chizhikov VE
[Ad] Endereço:Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM-470, Rockville, MD 20852, USA. dmitriy.volokhov@fda.hhs.gov
[Ti] Título:RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae.
[So] Source:Mol Phylogenet Evol;62(1):515-28, 2012 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Conventional classification of the species in the family Mycoplasmataceae is mainly based on phenotypic criteria, which are complicated, can be difficult to measure, and have the potential to be hampered by phenotypic deviations among the isolates. The number of biochemical reactions suitable for phenotypic characterization of the Mycoplasmataceae is also very limited and therefore the strategy for the final identification of the Mycoplasmataceae species is based on comparative serological results. However, serological testing of the Mycoplasmataceae species requires a performance panel of hyperimmune sera which contains anti-serum to each known species of the family, a high level of technical expertise, and can only be properly performed by mycoplasma-reference laboratories. In addition, the existence of uncultivated and fastidious Mycoplasmataceae species/isolates in clinical materials significantly complicates, or even makes impossible, the application of conventional bacteriological tests. The analysis of available genetic markers is an additional approach for the primary identification and phylogenetic classification of cultivable species and uncultivable or fastidious organisms in standard microbiological laboratories. The partial nucleotide sequences of the RNA polymerase ß-subunit gene (rpoB) and the 16S-23S rRNA intergenic transcribed spacer (ITS) were determined for all known type strains and the available non-type strains of the Mycoplasmataceae species. In addition to the available 16S rRNA gene data, the ITS and rpoB sequences were used to infer phylogenetic relationships among these species and to enable identification of the Mycoplasmataceae isolates to the species level. The comparison of the ITS and rpoB phylogenetic trees with the 16S rRNA reference phylogenetic tree revealed a similar clustering patterns for the Mycoplasmataceae species, with minor discrepancies for a few species that demonstrated higher divergence of their ITS and rpoB in comparison to their neighbor species. Overall, our results demonstrated that the ITS and rpoB gene could be useful complementary phylogenetic markers to infer phylogenetic relationships among the Mycoplasmataceae species and provide useful background information for the choice of appropriate metabolic and serological tests for the final classification of isolates. In summary, three-target sequence analysis, which includes the ITS, rpoB, and 16S rRNA genes, was demonstrated to be a reliable and useful taxonomic tool for the species differentiation within the family Mycoplasmataceae based on their phylogenetic relatedness and pairwise sequence similarities. We believe that this approach might also become a valuable tool for routine analysis and primary identification of new isolates in medical and veterinary microbiological laboratories.
[Mh] Termos MeSH primário: DNA Espaçador Ribossômico/genética
RNA Polimerases Dirigidas por DNA/genética
Mycoplasmataceae/genética
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Sequência de Bases
Teorema de Bayes
Evolução Molecular
Genes Bacterianos
Marcadores Genéticos
Funções Verossimilhança
Dados de Sequência Molecular
Mycoplasmataceae/classificação
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Ribosomal Spacer); 0 (Genetic Markers); 0 (RNA, Ribosomal, 16S); EC 2.7.7.6 (DNA-Directed RNA Polymerases); EC 2.7.7.6 (RNA polymerase beta subunit)
[Em] Mês de entrada:1204
[Cu] Atualização por classe:111219
[Lr] Data última revisão:
111219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111126
[St] Status:MEDLINE
[do] DOI:10.1016/j.ympev.2011.11.002



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